CN106718870A - 一种赤胫散叶片扦插繁殖方法 - Google Patents

一种赤胫散叶片扦插繁殖方法 Download PDF

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CN106718870A
CN106718870A CN201610960371.3A CN201610960371A CN106718870A CN 106718870 A CN106718870 A CN 106718870A CN 201610960371 A CN201610960371 A CN 201610960371A CN 106718870 A CN106718870 A CN 106718870A
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安树青
张霞
张红星
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Nanda (changshu) Research Institute Co Ltd
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Abstract

本发明一种赤胫散叶片扦插繁殖方法,包括以下具体步骤:步骤1. 配置初始诱导培养液和生根剂;步骤2. 初始诱导培养;步骤3. 愈伤组织诱导;步骤4. 增值培养;步骤5. 炼苗和移栽。通过上述,本发明的赤胫散叶片扦插繁殖方法,可以节约大量的扦插材料,不影响原植株的生长,摘取叶片省时省力。

Description

一种赤胫散叶片扦插繁殖方法
技术领域
本发明涉及植物无性繁殖方法技术领域,尤其涉及一种赤胫散叶片扦插的繁殖方法。
背景技术
赤胫散(拉丁名为Polygonum runcinatum )为蓼科蓼属草本植物,主产于四川、贵州、云南、湖南、湖北、陕西、甘肃及河南西部等地。适宜布置花境、路边或栽植于疏林下。
赤胫散对重金属镉、锌、铅和铜具有显著的耐性和富集特性,与此同时,赤胫散对污染土壤还有绿化覆盖,减少水土流失和重金属污染扩散的功能,为重金属污染土壤的植物修复开发了新的植物资源品种。
赤胫散常分株和种子繁殖,以分株繁殖为主,传统的方法是在出苗前,挖起根茎,分成单株,栽时,翻耕土地,开1.3m宽的高畦,按行、株距各约33cm开穴,每穴栽2株,填土区紧,施人畜粪水及草木灰,最后盖细土与畦面齐平。此方法一是需要大量的繁殖材料;二是对被取材的母树影响较大。
发明内容
本发明主要解决的技术问题是提供一种赤胫散叶片扦插繁殖方法,可以节约大量的扦插材料,不影响原植株的生长,摘取叶片省时省力。
为解决上述技术问题,本发明采用的一个技术方案是:提供了一种赤胫散叶片扦插繁殖方法,包括以下具体步骤:
步骤1. 配置初始诱导培养液和生根剂:使用蔗糖浓度为8%,琼脂成分为0.7%,pH值为5.6-5.8的溶液作为初始诱导培养液;使用NAA 400-800mg、维生素B1 2-5mg、蔗糖20-50g和硼砂3-6g加水1kg配成混合溶液作为生根剂,备用;
步骤2. 初始诱导培养:在夏、秋季上午或阴天时,选择当年成熟叶片,贴茎一刀快速剪下,在步骤1配制好的生根剂中速蘸后,插入配置的初始诱导培养液中至生根;
步骤3. 愈伤组织诱导:将步骤2培养出的生根赤胫散叶片插入愈伤组织诱导培养基中,并继续培养至新芽长出;
步骤4. 增值培养:将步骤3培养出的新芽剪下并转接到新芽增长和增值培养基中,培养至新芽长出并伸长到1cm以上;新芽增长和增值培养基组成为:MS+6-BA1.2-2.2 mg/L+NAA0.1-0.3 mg/L+IBA0.8-1.2 mg/L+KT0.8-1.2 mg/L;
步骤5. 炼苗和移栽:将珍珠岩、蛭石和黄沙以3:1:1的体积比例混合配置成苗床,将步骤4培养出的赤胫散幼苗转移到苗床上,保持温度20-25℃,湿度70%-80%,适当遮阴即可;7天生根,15天叶基部长出新芽,25-30天长出新叶,35天可移栽。
在本发明一个较佳实施例中,所述的步骤3中的愈伤组织诱导培养基的组成为:MS+6-BA1.0 mg/L+NAA0.2 mg/L。
在本发明一个较佳实施例中,所述的步骤3和步骤4中MS的培养基配方为:硝酸钾KNO3 1900mg/L,硝酸铵NH4NO3 1650mg/L,磷酸二氢钾KH2PO3 170mg/L,硫酸镁MgSO4·7H2O370mg/L;钙盐:氯化钙CaCl2·2H2O 440mg/L;微量元素:碘化钾KI 0.83mg/L,硼酸H3BO3 6.2mg/L,硫酸锰MnSO4·4H2O 22.3mg/L,硫酸锌ZnSO4·7H2O 8.6mg/L,钼酸钠Na2MoO4·2H2O 0.25mg/L,硫酸铜CuSO4·5H2O 0.025mg/L,氯化钴CoCl2·6H2O 0.025mg/L;铁盐:乙二胺四乙酸二钠Na2-EDTA 37.25mg/L,硫酸亚铁FeSO4·7H2O 27.85mg/L;有机酸:肌醇100mg/L,甘氨酸2 mg/L,盐酸硫胺素0.5 mg/L,盐酸吡哆醇0.5 mg/L,烟酸0.5 mg/L;蔗糖30g/L,琼脂粉7 g/L,pH为5.8。
在本发明一个较佳实施例中,所述的6-BA为6-苄氨基嘌呤。
在本发明一个较佳实施例中,所述的NAA为α-萘乙酸。
在本发明一个较佳实施例中,所述的IBA为吲哚-3-丁酸。
在本发明一个较佳实施例中,所述的KT为激动素。
本发明的有益效果是:本发明的一种赤胫散叶片扦插繁殖方法,可以节约大量的扦插材料,不影响原植株的生长,摘取叶片省时省力。
具体实施方式
下面将对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅是本发明的一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其它实施例,都属于本发明保护的范围。
本发明实施例包括:
一种赤胫散叶片扦插繁殖方法,包括以下具体步骤:
步骤1. 配置初始诱导培养液和生根剂:使用蔗糖浓度为8%,琼脂成分为0.7%,pH值为5.6-5.8的溶液作为初始诱导培养液;使用NAA(萘乙酸)400-800mg、维生素B1 2-5mg、蔗糖20-50g和硼砂3-6g加水1kg配成混合溶液作为生根剂,(NAA使用时先用少量95%的乙醇溶解后再配置成溶液),备用;
步骤2. 初始诱导培养:在夏、秋季上午或阴天时,选择当年成熟叶片,贴茎一刀快速剪下,在步骤1配制好的生根剂中速蘸后,插入配置的初始诱导培养液中至生根;
步骤3. 愈伤组织诱导:将步骤2培养出的生根赤胫散叶片插入愈伤组织诱导培养基中,并继续培养至新芽长出;
步骤4. 增值培养:将步骤3培养出的新芽剪下并转接到新芽增长和增值培养基中,培养至新芽长出并伸长到1cm以上;新芽增长和增值培养基组成为:MS+6-BA1.2-2.2 mg/L+NAA0.1-0.3 mg/L+IBA0.8-1.2 mg/L+KT0.8-1.2 mg/L;
步骤5. 炼苗和移栽:将珍珠岩、蛭石和黄沙以3:1:1的体积比例混合配置成苗床,将步骤4培养出的赤胫散幼苗转移到苗床上,保持温度20-25℃,湿度70%-80%,适当遮阴即可;7天生根,15天叶基部长出新芽,25-30天长出新叶,35天可移栽。
上述中,所述的步骤3中的愈伤组织诱导培养基的组成为:MS+6-BA1.0 mg/L+NAA0.2 mg/L。
其中,所述的步骤3和步骤4中MS的培养基配方为:硝酸钾KNO3 1900mg/L,硝酸铵NH4NO3 1650mg/L,磷酸二氢钾KH2PO3 170mg/L,硫酸镁MgSO4·7H2O 370mg/L;钙盐:氯化钙CaCl2·2H2O 440mg/L;微量元素:碘化钾KI 0.83mg/L,硼酸H3BO3 6.2mg/L,硫酸锰MnSO4·4H2O 22.3mg/L,硫酸锌ZnSO4·7H2O 8.6mg/L,钼酸钠Na2MoO4·2H2O 0.25mg/L,硫酸铜CuSO4·5H2O 0.025mg/L,氯化钴CoCl2·6H2O 0.025mg/L;铁盐:乙二胺四乙酸二钠Na2-EDTA37.25mg/L,硫酸亚铁FeSO4·7H2O 27.85mg/L;有机酸:肌醇100 mg/L,甘氨酸2 mg/L,盐酸硫胺素0.5 mg/L,盐酸吡哆醇0.5 mg/L,烟酸0.5 mg/L;蔗糖30 g/L,琼脂粉7 g/L,pH为5.8。
进一步的,所述的6-BA为6-苄氨基嘌呤;所述的NAA为α-萘乙酸;所述的IBA为吲哚-3-丁酸;所述的KT为激动素。
本发明的一种赤胫散叶片扦插繁殖方法相比于现有技术具有如下优点:
1、用叶片扦插繁殖可以节约大量的扦插材料;
2、用叶片扦插繁殖不影响原植株的生长;
3、摘取叶片省时省力。
综上所述,本发明的一种赤胫散叶片扦插繁殖方法,可以节约大量的扦插材料,不影响原植株的生长,摘取叶片省时省力。
以上所述仅为本发明的实施例,并非因此限制本发明的专利范围,凡是利用本发明说明书内容所作的等效结构或等效流程变换,或直接或间接运用在其它相关的技术领域,均同理包括在本发明的专利保护范围内。

Claims (7)

1.一种赤胫散叶片扦插繁殖方法,其特征在于,包括以下具体步骤:
步骤1. 配置初始诱导培养液和生根剂:使用蔗糖浓度为8%,琼脂成分为0.7%,pH值为5.6-5.8的溶液作为初始诱导培养液;使用NAA 400-800mg、维生素B1 2-5mg、蔗糖20-50g和硼砂3-6g加水1kg配成混合溶液作为生根剂,备用;
步骤2. 初始诱导培养:在夏、秋季上午或阴天时,选择当年成熟叶片,贴茎一刀快速剪下,在步骤1配制好的生根剂中速蘸后,插入配置的初始诱导培养液中至生根;
步骤3. 愈伤组织诱导:将步骤2培养出的生根赤胫散叶片插入愈伤组织诱导培养基中,并继续培养至新芽长出;
步骤4. 增值培养:将步骤3培养出的新芽剪下并转接到新芽增长和增值培养基中,培养至新芽长出并伸长到1cm以上;新芽增长和增值培养基组成为:MS+6-BA1.2-2.2mg/L+NAA0.1-0.3mg/L+IBA0.8-1.2mg/L+KT0.8-1.2mg/L;
步骤5. 炼苗和移栽:将珍珠岩、蛭石和黄沙以3:1:1的体积比例混合配置成苗床,将步骤4培养出的赤胫散幼苗转移到苗床上,保持温度20-25℃,湿度70%-80%,适当遮阴即可;7天生根,15天叶基部长出新芽,25-30天长出新叶,35天可移栽。
2.根据权利要求1所述的赤胫散叶片扦插繁殖方法,其特征在于,所述的步骤3中的愈伤组织诱导培养基的组成为:MS+6-BA1.0mg/L+NAA0.2mg/L。
3.根据权利要求1或2所述的赤胫散叶片扦插繁殖方法,其特征在于,所述的步骤3和步骤4中MS的培养基配方为:硝酸钾KNO3 1900mg/L,硝酸铵NH4NO3 1650mg/L,磷酸二氢钾KH2PO3 170mg/L,硫酸镁MgSO4·7H2O 370mg/L;钙盐:氯化钙CaCl2·2H2O 440mg/L;微量元素:碘化钾KI 0.83mg/L,硼酸H3BO3 6.2mg/L,硫酸锰MnSO4·4H2O 22.3mg/L,硫酸锌ZnSO4·7H2O 8.6mg/L,钼酸钠Na2MoO4·2H2O 0.25mg/L,硫酸铜CuSO4·5H2O 0.025mg/L,氯化钴CoCl2·6H2O 0.025mg/L;铁盐:乙二胺四乙酸二钠Na2-EDTA 37.25mg/L,硫酸亚铁FeSO4·7H2O 27.85mg/L;有机酸:肌醇100 mg/L,甘氨酸2 mg/L,盐酸硫胺素0.5 mg/L,盐酸吡哆醇0.5 mg/L,烟酸0.5 mg/L;蔗糖30 g/L,琼脂粉7 g/L,pH为5.8。
4.根据权利要求1或2所述的赤胫散叶片扦插繁殖方法,其特征在于,所述的6-BA为6-苄氨基嘌呤。
5.根据权利要求1或2所述的赤胫散叶片扦插繁殖方法,其特征在于,所述的NAA为α-萘乙酸。
6.根据权利要求1或2所述的赤胫散叶片扦插繁殖方法,其特征在于,所述的IBA为吲哚-3-丁酸。
7.根据权利要求1所述的赤胫散叶片扦插繁殖方法,其特征在于,所述的KT为激动素。
CN201610960371.3A 2016-11-04 2016-11-04 一种赤胫散叶片扦插繁殖方法 Pending CN106718870A (zh)

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