CN106676037A - Bacterial growth accelerant and preparation method thereof - Google Patents

Bacterial growth accelerant and preparation method thereof Download PDF

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Publication number
CN106676037A
CN106676037A CN201611134348.5A CN201611134348A CN106676037A CN 106676037 A CN106676037 A CN 106676037A CN 201611134348 A CN201611134348 A CN 201611134348A CN 106676037 A CN106676037 A CN 106676037A
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China
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parts
bacterial growth
spermidine
xanthan gum
mantoquita
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CN201611134348.5A
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邓勤
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Qinzhou University
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Qinzhou University
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Priority to CN201611134348.5A priority Critical patent/CN106676037A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a bacterial growth accelerant and a preparation method thereof. The bacterial growth accelerant is prepared from the following formula components in parts by weight: 4 to 5 parts of calcium salt, 13 to 15 parts of a metal salt solution, 3 to 5 parts of a humectant, 4 to 6 parts of a leavening agent, 3 to 7 parts of ferrite, 3 to 7 parts of hydroxylamine hydrochloride, 4 to 6 parts of lemon essential oil, 1 to 3 parts of hydroxylamine sulphate, 3 to 5 parts of copper salt, 5 to 7 parts of spermine, 6 to 10 parts of spermidine, 6 to 8 parts of xanthan gum and 10 to 12 parts of saccharose. The bacterial growth accelerant is simple in formula and easy to prepare, can be used in the cultivation process of nitrifying bacteria, and can also be directly dropped into a sewage disposal system, so that the nitrifying bacteria can be grown and propagated quickly in short time, and the cultured nitrifying bacteria are high in activity and high in impact resistance.

Description

A kind of bacterial growth promoter and preparation method thereof
Technical field
The present invention relates to antibacterial culturing technical field, in particular to a kind of bacterial growth promoter and its preparation side Method.
Background technology
In biological denitrification method, either traditional nitrification-denitrification, or new short-cut nitrification-denitrification and short Journey nitration-anaerobic ammoxidation all needs the Nitrification removal of ammonia and nitrogen through nitrobacteria.It is micro- that nitrobacteria belongs to chemotrophy type It is biological.Biological cell can only utilize the energy preserved with forms such as ATP, it is impossible to the freedom for directly being discharged using chemical reaction Energy.In aerobic metabolism, ATP is mainly synthesized by the oxidative phosphorylation of respiratory chain.Ammoxidation phosphorylation efficiency is very low, institute The ATP that can be produced is very limited, and these energy are mainly used in electron transition to higher energy level, and this causes nitrobacteria growth very slow Slowly, phase generation is 8~36h.Peptidoglycan content is low in the cell wall of nitrobacteria, and protein and fat content are high, therefore to ring Border change is more sensitive, and nitrobacteria adaptability and toleration natural in nature is poor, cannot be with the conditions of a lot Heterotroph microorganism gets the mastery in growth competition.In sewage disposal system, when nitrobacteria content is relatively low in activated sludge When, fast-growth cannot breed within a short period of time by the environmental condition such as dissolved oxygen and pH is adjusted, ultimately result in existing operation Sewage disposal system removal of ammonia and nitrogen it is limited in one's ability, industrially generally can adopt in sewage disposal system directly throw in train The high concentration nitrobacteria supported is solving this problem.Either directly in sewage disposal system cultivate nitrobacteria still Nitrobacteria is cultivated outside sewage disposal system, is required for adding the material for promoting growth, the growth of nitrobacteria could be accelerated Speed.
The content of the invention
The invention provides a kind of bacterial growth promoter and preparation method thereof, simply, it is easy to prepare, can for inventive formulation In for the incubation of nitrobacteria, it is also possible to be directly added in sewage disposal system, nitrobacteria is enable in short-term Interior fast-growth breeding, the nitrobacteria activity cultivated is high, tolerance impact capacity is strong.
For achieving the above object, the present invention provides following technical scheme:
A kind of bacterial growth promoter and preparation method thereof, is made up of following mass fraction formula components:Calcium salt 4-5 parts, Metal salt solution 13-15 parts, wetting agent 3-5 parts, leaven 4-6 parts, ferrous salt 3-7 part, sour azanol 3-7 parts, Fructus Citri Limoniae quintessence oil 4-6 Part, oxammonium sulfate. 1-3 parts, mantoquita 3-5 parts, spermine 5-7 parts, spermidine 6-10 parts, xanthan gum 6-8 parts, sucrose 10-12 parts.
Further:It is made up of following mass fraction formula components:4 parts of calcium salt, 13 parts of metal salt solution, wetting agent 3 part, 4 parts of ferment agent, 3 parts of ferrous salt, sour 3 parts of azanol, 4 parts of Fructus Citri Limoniae quintessence oil, 1 part of oxammonium sulfate., 3 parts of mantoquita, 5 parts of spermine, spermidine 6 Part, 6 parts of xanthan gum, 10 parts of sucrose.
Further:It is made up of following mass fraction formula components:4.5 parts of calcium salt, 14 parts of metal salt solution, 4 parts of wetting agent, 5 parts of leaven, 5 parts of ferrous salt, sour 5 parts of azanol, 5 parts of Fructus Citri Limoniae quintessence oil, 2 parts of oxammonium sulfate., 4 parts of mantoquita, 6 parts of spermine, spermidine 8 Part, 7 parts of xanthan gum, 11 parts of sucrose.
Further:It is made up of following mass fraction formula components:5 parts of calcium salt, 15 parts of metal salt solution, wetting agent 5 part, 6 parts of ferment agent, 7 parts of ferrous salt, sour 7 parts of azanol, 6 parts of Fructus Citri Limoniae quintessence oil, 3 parts of oxammonium sulfate., 5 parts of mantoquita, 7 parts of spermine, spermidine 10 Part, 8 parts of xanthan gum, 12 parts of sucrose.
Further:Preparation method of the present invention has following steps:
Step one, formula is distributed according to aforementioned proportion, it is standby;
Step 2, above-mentioned calcium salt, ferrous salt, sour azanol, oxammonium sulfate., mantoquita, spermidine, xanthan gum, sucrose are added Mixed in metallic solution;
Step 3, the solution for mixing is added wetting agent, mix homogeneously after leaven;
Step 4, the product fill for preparing step 3 are packed after standing, you can.
The invention has the beneficial effects as follows:
1st, inventive formulation is simple, and it is easy to prepare, in can be used for the incubation of nitrobacteria;
2nd, can also directly be added in sewage disposal system, enable nitrobacteria fast-growth breeding at short notice;
3rd, active high, the tolerance impact capacity of nitrobacteria cultivated is strong.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, Obviously, described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.Based in the present invention Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all Belong to the scope of protection of the invention.
Embodiment one:
A kind of bacterial growth promoter is made up of following mass fraction formula components:Calcium salt 4-5 parts, metal salt solution 13- 15 parts, wetting agent 3-5 parts, leaven 4-6 parts, ferrous salt 3-7 part, sour azanol 3-7 parts, Fructus Citri Limoniae quintessence oil 4-6 parts, oxammonium sulfate. 1- 3 parts, mantoquita 3-5 parts, spermine 5-7 parts, spermidine 6-10 parts, xanthan gum 6-8 parts, sucrose 10-12 parts.
Embodiment two:
A kind of bacterial growth promoter is made up of following mass fraction formula components:4 parts of calcium salt, 13 parts of metal salt solution, 3 parts of wetting agent, 4 parts of leaven, 3 parts of ferrous salt, sour 3 parts of azanol, 4 parts of Fructus Citri Limoniae quintessence oil, 1 part of oxammonium sulfate., 3 parts of mantoquita, spermine 5 Part, 6 parts of spermidine, 6 parts of xanthan gum, 10 parts of sucrose.
Embodiment three:
A kind of bacterial growth promoter is made up of following mass fraction formula components:4.5 parts of calcium salt, metal salt solution 14 Part, 4 parts of wetting agent, 5 parts of leaven, 5 parts of ferrous salt, sour 5 parts of azanol, 5 parts of Fructus Citri Limoniae quintessence oil, 2 parts of oxammonium sulfate., 4 parts of mantoquita, essence 6 parts of amine, 8 parts of spermidine, 7 parts of xanthan gum, 11 parts of sucrose.
Example IV:
A kind of bacterial growth promoter is made up of following mass fraction formula components:5 parts of calcium salt, 15 parts of metal salt solution, 5 parts of wetting agent, 6 parts of leaven, 7 parts of ferrous salt, sour 7 parts of azanol, 6 parts of Fructus Citri Limoniae quintessence oil, 3 parts of oxammonium sulfate., 5 parts of mantoquita, spermine 7 Part, 10 parts of spermidine, 8 parts of xanthan gum, 12 parts of sucrose.
Embodiment five:
A kind of preparation method preparation method of bacterial growth promoter comprises the steps:
Step one, formula is distributed according to aforementioned proportion, it is standby;
Step 2, above-mentioned calcium salt, ferrous salt, sour azanol, oxammonium sulfate., mantoquita, spermidine, xanthan gum, sucrose are added Mixed in metallic solution;
Step 3, the solution for mixing is added wetting agent, mix homogeneously after leaven;
Step 4, the product fill for preparing step 3 are packed after standing, you can.
Moreover, it will be appreciated that although this specification is been described by according to embodiment, not each embodiment is only wrapped Containing an independent technical scheme, this narrating mode of description is only that for clarity those skilled in the art should Using description as an entirety, the technical scheme in each embodiment can also Jing it is appropriately combined, form those skilled in the art Understandable other embodiment.

Claims (5)

1. a kind of bacterial growth promoter, it is characterised in that:It is made up of following mass fraction formula components:
Calcium salt 4-5 parts, metal salt solution 13-15 parts, wetting agent 3-5 parts, leaven 4-6 parts, ferrous salt 3-7 part, sour azanol 3-7 Part, Fructus Citri Limoniae quintessence oil 4-6 parts, oxammonium sulfate. 1-3 parts, mantoquita 3-5 parts, spermine 5-7 parts, spermidine 6-10 parts, xanthan gum 6-8 parts, Sucrose 10-12 parts.
2. a kind of bacterial growth promoter according to claim 1, it is characterised in that:By following mass fraction formula components Composition:
4 parts of calcium salt, 13 parts of metal salt solution, 3 parts of wetting agent, 4 parts of leaven, 3 parts of ferrous salt, sour 3 parts of azanol, Fructus Citri Limoniae quintessence oil 4 Part, 1 part of oxammonium sulfate., 3 parts of mantoquita, 5 parts of spermine, 6 parts of spermidine, 6 parts of xanthan gum, 10 parts of sucrose.
3. a kind of bacterial growth promoter according to claim 1, it is characterised in that:By following mass fraction formula components Composition:
4.5 parts of calcium salt, 14 parts of metal salt solution, 4 parts of wetting agent, 5 parts of leaven, 5 parts of ferrous salt, sour 5 parts of azanol, Fructus Citri Limoniae quintessence oil 5 parts, 2 parts of oxammonium sulfate., 4 parts of mantoquita, 6 parts of spermine, 8 parts of spermidine, 7 parts of xanthan gum, 11 parts of sucrose.
4. a kind of bacterial growth promoter according to claim 1, it is characterised in that:By following mass fraction formula components Composition:
5 parts of calcium salt, 15 parts of metal salt solution, 5 parts of wetting agent, 6 parts of leaven, 7 parts of ferrous salt, sour 7 parts of azanol, Fructus Citri Limoniae quintessence oil 6 Part, 3 parts of oxammonium sulfate., 5 parts of mantoquita, 7 parts of spermine, 10 parts of spermidine, 8 parts of xanthan gum, 12 parts of sucrose.
5. a kind of a kind of preparation method of bacterial growth promoter according to claim 1, it is characterised in that:Including as follows Step:
Step one, formula is distributed according to aforementioned proportion, it is standby;
Step 2, above-mentioned calcium salt, ferrous salt, sour azanol, oxammonium sulfate., mantoquita, spermidine, xanthan gum, sucrose are added into metal Mixed in solution;
Step 3, the solution for mixing is added wetting agent, mix homogeneously after leaven;
Step 4, the product fill for preparing step 3 are packed after standing, you can.
CN201611134348.5A 2016-12-10 2016-12-10 Bacterial growth accelerant and preparation method thereof Pending CN106676037A (en)

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CN106676037A true CN106676037A (en) 2017-05-17

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105624086A (en) * 2014-10-28 2016-06-01 中国石油化工股份有限公司 Nitrifying bacteria growth promoter and preparation method thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105624086A (en) * 2014-10-28 2016-06-01 中国石油化工股份有限公司 Nitrifying bacteria growth promoter and preparation method thereof

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Application publication date: 20170517