CN116375222A - Sustained-release composite microbial agent, preparation method and application thereof in treating black and odorous water body - Google Patents
Sustained-release composite microbial agent, preparation method and application thereof in treating black and odorous water body Download PDFInfo
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- CN116375222A CN116375222A CN202310321174.7A CN202310321174A CN116375222A CN 116375222 A CN116375222 A CN 116375222A CN 202310321174 A CN202310321174 A CN 202310321174A CN 116375222 A CN116375222 A CN 116375222A
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Images
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
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- C12R2001/07—Bacillus
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a slow-release compound microbial agent, a preparation method and application thereof in treating black and odorous water, which relate to the technical field of environmental treatment and comprise the following components in parts by weight: 2-20 parts of dry material mixture and 1 part of slow release agent, wherein the dry material mixture consists of the following components: 1 part of mixed bacterial powder and 2 to 5 parts of auxiliary materials; preparing a fungus powder mixture in advance, preparing auxiliary materials, preparing a dry material mixture, preparing a slow release agent solution, and preparing a composite microbial agent. The compound microbial agent directly acts on the middle-lower water layer and the bottom mud, controls the slow release speed to realize rapid coagulation and clarification of the severe black and odorous water body, and slowly releases the electronic receptor and the microbial agent, and finally achieves the purposes of one-time adding of the microbial agent, long-acting treatment of the black and odorous water body and water ecological reconstruction.
Description
Technical Field
The invention relates to the technical field of environmental treatment, in particular to a slow-release compound microbial agent, a preparation method and application thereof in treating black and odorous water bodies.
Background
In recent years, along with the improvement of the living standard of people and the relatively lagging factors of water treatment infrastructure construction, a large amount of pollutants enter urban river channels and rural water bodies, the water pollution crisis of urban river channel black and odor, rural water body black and odor and the like is caused, and the drinking water safety and physical and mental health of people are seriously influenced.
The black and odorous water body is black or black in color, can stimulate olfactory organs of people on sense of smell, causes unpleasant or aversive smell of people, and is a complex process related to physics, chemistry and biology. It is believed that the deficiency of the electron acceptors such as dissolved oxygen in water is the most direct cause of black and odorous water. In particular in the anoxic layer at the lower part (underlaying water and bottom mud) of the water body, the dissolved oxygen exchange of the atmosphere is limited, and a large amount of organic matters are deposited and SO is generated 4 2- As electron acceptors, reduced sulfate bacteriaGradually reduce to S 2- . Meanwhile, metal ions such as iron, manganese and the like are reduced into Fe by other microorganisms 2+ 、Mn 2+ Isoreduced ion, S at bottom 2- With Fe 2+ 、Mn 2+ The metal sulfide generated by combining the plasma metal ions is released and suspended in the overlying water body along with the disturbance of the water body, so that the water body is blackened. Therefore, the black and odorous water body mainly solves the problem of insufficient electron acceptors at the lower layer in the water body.
At present, common black and odorous water body treatment modes comprise aeration oxygenation, bottom mud dredging, independent dosing of a medicament or a bacterial agent and the like. The aeration method and the sediment dredging method have the problems of large engineering quantity, high requirements on equipment and energy consumption, large emission of odor along with the aeration process, secondary treatment of the sediment and the like. The microbial inoculum and the agent are independently added, but the workload is small, but the agent and the microbial inoculum are added to have the problems of little effect on the middle-lower water body, easy secondary pollution of the agent, difficult residence of the agent and the microbial inoculum, short action time, repeated addition and the like.
For the problems in the related art, no effective solution has been proposed at present.
Disclosure of Invention
Aiming at the problems in the related art, the invention provides a slow-release compound microbial agent, a preparation method and application thereof in treating black and odorous water bodies, and the purposes of rapid coagulation and clarification, electron acceptor and microbial agent slow release of severe black and odorous water bodies, one-time adding of the compound microbial agent, long-acting treatment of black and odorous water bodies and water ecological reconstruction are finally achieved by acting the compound microbial agent on a middle-lower water layer and bottom mud and controlling slow release speed.
The technical scheme of the invention is realized as follows:
in one aspect of the invention:
the slow-release composite microbial agent consists of the following components in parts by weight: 2-20 parts of dry material mixture and 1 part of slow release agent, wherein;
the dry material mixture consists of the following components: 1 part of mixed bacterial powder and 2 to 5 parts of auxiliary materials.
Further, the mixed bacterial powder consists of the following components: 4-8 parts of bacillus powder, 1-3 parts of nitrifying bacteria powder and 1-3 parts of denitrifying bacteria powder.
Further, the auxiliary materials comprise the following components: 2 to 6 parts of electron acceptor, 1 to 4 parts of coagulant and 0.3 part of microorganism promoter.
Further, the electron acceptor is any one of calcium nitrate, calcium peroxide or sodium percarbonate.
Further, the coagulant consists of the following components: 2 to 1 part of Polyacrylamide (PAM) and 1 part of polyaluminum chloride (PAC).
Further, the microbial promoter consists of the following components: 1 part of cellulose B, 1 part of cysteine and 1 part of sodium dihydrogen phosphate.
Further, the slow release agent is polylactic acid.
In another aspect of the invention:
the preparation method of the slow-release compound microbial agent comprises the following steps:
step S1, preparing a bacterial powder mixture in advance, and respectively inoculating bacillus, nitrifying bacteria and denitrifying bacteria strains into a culture solution; then adding 5-30 g of microorganism carrier sterilized for 30min at 121 ℃ per 100mL of culture solution, and continuing to culture for 2-5 days to enable microorganisms to adhere to the carrier; obtaining a carrier mixture to which the strain is attached; flooding the obtained carrier mixture with a solution containing 5-15% of skimmed milk powder for freezing and icing; freeze-drying the frozen carrier mixture to obtain bacterial powder of three strains respectively; uniformly mixing the dried bacterial powder of the three strains of bacteria to obtain a bacterial powder mixture;
s2, preparing auxiliary materials, adding an electron acceptor, a coagulant and a microbial promoter into a grinder, and fully grinding and uniformly mixing to obtain the auxiliary materials;
step S3, preparing a dry material mixture, and respectively and fully mixing and uniformly stirring the prepared fungus powder mixture and auxiliary materials to obtain the dry material mixture;
s4, preparing a slow release agent solution, adding the slow release agent into dichloromethane, soaking for 1-3 hours, and carrying out cold water ultrasonic treatment to obtain the slow release agent solution;
and S5, preparing a composite microbial agent, and pouring the prepared dry material mixture into a slow-release agent solution to be fully stirred and uniformly mixed to obtain the composite microbial agent.
Wherein, the preparation of the compound microbial agent further comprises the following steps:
s6, molding the composite microbial agent, and adding the composite microbial agent into a tablet press or a granulator to be pressed into tablets or granulated to obtain a tablet or granular slow-release crude product of the composite microbial agent;
and S7, drying the crude product of the composite microbial agent in a room temperature or oven at 35+/-2 ℃ for 7-10 hours to obtain a sheet or granular slow-release composite microbial agent finished product.
In another aspect of the invention:
the application of the slow-release compound microbial agent in treating black and odorous water bodies comprises the following steps:
and throwing the prepared flaky or granular slow-release composite microbial agent finished product into black and odorous water to be treated, and detecting the water quality periodically.
The invention has the beneficial effects that:
the invention adopts a mode that microorganisms and carriers are firstly compounded and coated to prepare the compound microorganism microbial inoculum, so that the microorganisms are attached to the surfaces and internal gaps of the carriers, the carriers not only adsorb nutrient substances and can keep the activity of the microorganisms for a long time, but also are easier to reside on the surfaces of the water body and the bottom mud after being thrown into the water body, and the degradation and denitrification effects of the microorganisms on organic matters in the lower water body or sediment are exerted; meanwhile, the porous structure of the added carrier can adsorb certain pollutants, so that the removal efficiency of the pollutants is improved, the water body restoration progress is accelerated, and finally, the pollutants are thoroughly decomposed and removed by microorganisms on the surface of the carrier, so that the rapid restoration of the seriously polluted black and odorous water body is realized; then degrading the deposited organic matters by the microorganisms and the electron acceptors released by the composite microbial agent, denitrifying and deodorizing; the composite microbial agent added to the water body with less serious pollution quickly sinks, slowly releases calcium peroxide, recovers dissolved oxygen at the bottom layer of the water body, directly acts on the middle-lower water layer and bottom mud, controls the slow release speed to realize quick coagulation and clarification of the severe black and odorous water body, and slowly releases the electron acceptor and the microbial agent, and finally achieves the purposes of one-time adding of the microbial agent, long-acting treatment of the black and odorous water body and water ecological reconstruction.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a finished product diagram of a slow-release type composite microbial agent according to an embodiment of the present invention;
FIG. 2 is a diagram showing the oxygen release of a peroxide type slow release microbial agent of a slow release type composite microbial agent according to an embodiment of the present invention;
FIG. 3 is a graph showing the comparison of microbial release conditions of a slow-release type composite microbial agent at 5 days and at 10 days according to an embodiment of the present invention;
fig. 4 is a graph showing the effect of a slow-release compound microbial agent on treating black and odorous water with different concentrations in treating black and odorous water according to an embodiment of the invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which are derived by a person skilled in the art based on the embodiments of the invention, fall within the scope of protection of the invention.
According to the embodiment of the invention, a slow-release compound microbial agent, a preparation method and application thereof in treating black and odorous water bodies are provided.
The present invention provides two electron acceptor schemes: calcium nitrate and calcium peroxide or sodium percarbonate, respectively, to obtain two microbial inoculum products: the nitrate type slow-release composite microbial inoculum and the peroxy type slow-release composite microbial inoculum can be added in a targeted manner according to different water pollution characteristics.
According to the technical scheme, for the situation that the pollution of refractory organic matters is serious and black and odorous water is not solved all the year round, the refractory organic matters can be degraded by adopting the strong oxidizing substances released by the peroxide type slow-release composite microbial inoculum; for black and odorous water bodies polluted by organic matters and nitrogen sources, the peroxide type slow-release composite microbial agent can be used for converting macromolecular organic matters into micromolecular organic matters and converting ammonia nitrogen into nitrate, and after 5-10 days, the nitrate type slow-release composite microbial agent is applied, and the nitrate and denitrifying bacteria are used for removing the organic matters and denitrifying denitrification; for water bodies with higher turbidity, the coagulation effect can be accelerated by increasing the content of the disintegrating agent and the coagulant, so that the black and odorous water can be clarified rapidly.
Embodiment one: the preparation of the nitrate type slow-release composite microbial inoculum comprises the following specific steps:
step S1, preparing a mixture of bacteria amplification and bacterial powder: firstly, inoculating three strains of bacterial powder of the purchased bacillus, nitrifying bacteria and denitrifying bacteria into nutrient broth culture solution with proper volume according to the inoculum size of 1% of mass ratio, and performing expansion culture for 24 hours under the conditions of 30-37 ℃ and stirring; then, 50g of diatomaceous earth sterilized at 121℃for 30 minutes was added to 100mL of the culture solution, and the culture was continued for 3 days, whereby microorganisms were allowed to adhere to the carrier. Next, the carrier mixture to which the three strains were attached was harvested by centrifugation at 4500rpm for 5min using a centrifugal dehydrator. Again, the obtained carrier mixture was just submerged in a solution containing 5% nonfat dry milk, and frozen in a refrigerator overnight; then, freeze-drying the frozen bacteria and bentonite mixture for 48 hours to obtain bacterial powder of three strains respectively; and finally, uniformly mixing the dried three bacterial powders according to the mass ratio of 4:1:1 to obtain a bacterial powder mixture.
Step S2, preparing auxiliary materials: adding 6 parts of calcium nitrate, 2 parts of coagulant (prepared from polyacrylamide and polyaluminium chloride according to the mass ratio of 1:1) and 0.3 part of microbial accelerator (prepared from vitamin B, cysteine and sodium dihydrogen phosphate according to the mass ratio of 1:1:1) into a grinder, and fully grinding and uniformly mixing to obtain auxiliary materials.
Step S3, preparing a mixture of bacterial powder and auxiliary materials: mixing the bacterial powder obtained in the step S1 and the auxiliary material obtained in the step S2 according to the mass ratio of 1:2, adding 0.5% of disintegrating agent, improving the release rate, and fully and uniformly stirring to obtain a dry material consisting of a mixture of the bacterial powder and the auxiliary material.
Step S4, preparing a slow release agent: polylactic acid is preferably used as a slow release agent, the polylactic acid is added into methylene dichloride according to the mass volume ratio of 2.0g to 10mL, and after soaking for 1-3 hours, the pasty mixture is obtained by cold water ultrasonic treatment.
Step S5, preparing a composite microbial inoculum: pouring the dry material into a slow release agent solution, mixing the dry material and polylactic acid in a mass ratio of 10:1, and fully stirring and uniformly mixing by adopting a shearing type stirrer to obtain coarse material; and when the pasty coarse material is still in a softened state, adding the pasty coarse material into a tablet press to be pressed into tablets, and obtaining a tablet slow-release composite microbial agent coarse product.
Step S6, forming and storing the microbial inoculum: drying the slow-release composite microbial agent crude product obtained in the step S5 for 7-10 hours at room temperature to obtain a tablet nitrate type slow-release composite microbial agent finished product; the microbial inoculum can be placed in shade or refrigerated. The finished product of the microbial inoculum is shown in figure 1.
Embodiment two: the preparation of the peroxide type slow-release composite microbial inoculum comprises the following specific implementation steps:
step S1, preparing a mixture of bacteria amplification and bacterial powder: the procedure of example 1 is the same as that of the related steps such as the amplification of the bacterial powder; and uniformly mixing the bacillus, nitrifying bacteria and denitrifying bacteria powder obtained by drying according to the mass ratio of 4:2:1 to obtain a bacterial powder mixture.
Step S2, preparing auxiliary materials: adding 6 parts of calcium peroxide, 2 parts of coagulant (prepared from polyacrylamide and polyaluminium chloride according to the mass ratio of 2:1) and 0.3 part of microbial accelerator (prepared from vitamin B, cysteine and sodium dihydrogen phosphate according to the mass ratio of 1:1:1) into a grinder, and fully grinding and uniformly mixing to obtain auxiliary materials.
Step S3, preparing a dry material: and (3) mixing the bacterial powder obtained in the step (S1) and the auxiliary material obtained in the step (S2) according to the mass ratio of 1:2, and fully and uniformly stirring to obtain a dry material consisting of the mixture of the bacterial powder and the auxiliary material.
Step S4, preparing a slow release agent: polylactic acid is preferably used as a slow release agent, the polylactic acid is added into methylene dichloride according to the mass volume ratio of 2.0g to 10mL, and after soaking for 1-3 hours, the pasty mixture is obtained by cold water ultrasonic treatment.
Step S5, preparing a composite microbial inoculum: adding a dry material into a slow release agent solution, mixing the dry material and polylactic acid in a mass ratio of 10:1, and fully stirring and uniformly mixing by a shearing type stirrer to obtain coarse material; and when the coarse material is still in a softened state, adding the coarse material into a tablet press to be pressed into tablets, so as to obtain a tablet-shaped peroxy slow-release compound microbial agent coarse product.
Step S6, forming and storing the microbial inoculum: and (3) carrying out ventilation drying on the slow-release composite microbial agent crude product obtained in the step (S4) for 8 hours at room temperature to obtain the sheet-shaped peroxide slow-release composite microbial agent, and putting the sheet-shaped peroxide slow-release composite microbial agent into clear water to periodically monitor the oxygen release condition, wherein the oxygen release condition is shown in figure 2.
Embodiment III: the nitrate type slow-release composite microbial agent is statically released by nitrate and microorganisms, as shown in figure 3, and comprises the following specific steps:
step S1, preparation of a microbial inoculum: according to the preparation scheme of the embodiment 1, a finished product of the tablet-shaped slow-release compound microbial agent is obtained.
Step S2, releasing clear water: taking 4500 mL conical flasks, adding 500mL deionized water (pure water or ultrapure water) into each conical flask, carrying out grouping numbers 1#, 2#, 3# and 4#, sealing and wrapping with more than 6 layers of gauze, sterilizing at 121 ℃ for 30min, and cooling to room temperature for later use. 5 bacteria agents are respectively added into each bottle (the total amount of tablets is weighed before adding and recorded), 4 bottles are sealed by rubber plugs and placed horizontally to simulate the release conditions of bacteria agent nitrate and bacteria under still water conditions. 10mL of water samples were taken from bottles # 1 and # 2 at 1 day intervals to measure the nitrate concentration in the water. The water in the conical flask was then decanted, leaving the tablet, replaced with 500mL fresh deionized water, and the water was sampled continuously. Multiplying the measured nitrate concentration by the deionized volume in the conical flask to obtain release amount, and calculating the nitrate release condition of the medicament after superposition; and taking 0.1mL of water sample for dilution after uniformly mixing in 3# and 4# bottles every 5 days, coating a flat plate, then placing the flat plate into an incubator for 3 days at 37 ℃ for culturing, and observing the release and growth conditions of bacteria.
The embodiment shows that the prepared microbial inoculum can keep a good and complete form within 25 days under the action of still water, the nitrate release time can reach more than 30 days, and the nitrate release rate can reach 0.6-1.3 mg-N/(g microbial inoculum. D).
Embodiment four: the nitrate type slow-release composite microbial agent nitrate and the running water release of microorganisms comprise the following steps:
step S1, preparation of a microbial inoculum: according to the manufacturing scheme of the first embodiment, a finished product of the tablet-shaped slow-release compound microbial agent is obtained.
Step S2, releasing clear water: taking 4500 mL conical flasks, no. 1, no. 2, no. 3 and No. 4, adding 500mL deionized water (pure water or ultrapure water) into each conical flask, sealing and wrapping with more than 6 layers of gauze, sterilizing at 121deg.C for 30min, and cooling to room temperature for use. To each bottle, 5 pieces of the microbial inoculum (the total amount of the tablets before addition was weighed and recorded, and the final weights were similar) were added separately and sealed with a rubber stopper. Then, the microbial inoculum is released under the condition of flowing water by passing through a stirring paddle from a small hole on the rubber plug, connecting the stirring paddle, controlling the stirring speed to be 100rpm, and simulating the release condition of the microbial inoculum under the condition of flowing water. After stopping the stirrer every day, the condition of the tablets was photographed and recorded, and registration and photo preservation were made. Taking 10mL of water samples from the 1# and 2# bottles every 1 day, measuring the nitrate concentration in the water, pouring out the water in the conical bottle, leaving tablets, replacing 500mL of fresh deionized water, and calculating the nitrate release condition of the medicament; 1mL of water samples from 3# and 4# bottles were diluted every 5 days, plated, and then placed in an incubator at 37℃for cultivation, and bacterial growth was observed daily.
The embodiment shows that the prepared microbial inoculum can keep a good and complete form within 19 days under the action of still water and running water, the nitrate release time can reach more than 25 days, and the nitrate release rate can reach 1.9-3.7 mg-N/(g microbial inoculum. D).
Fifth embodiment: the release of the oxygen and the microorganism of the peroxide type slow-release composite microbial agent comprises the following steps:
step S1, preparation of a microbial inoculum: according to the manufacturing scheme of the second embodiment, a finished product of the tablet-shaped slow-release compound microbial agent is obtained.
Step S2, releasing clear water: taking 7 500mL conical flasks, adding 500mL deionized water (pure water or ultrapure water) into each conical flask, carrying out grouping number 1# to 7# and sterilizing for 30min at 121 ℃ by sealing and wrapping with more than 6 layers of gauze, and cooling to room temperature for later use. 1 bacterial agent is respectively added into six bottles of No. 2-7 (the total amount of tablets is weighed and recorded before adding, the final weight is similar), then high-purity argon is used for blowing oxygen out of the bottles, and after the dissolved oxygen is reduced to 0mg/L, the 7 bottles are sealed by sealing films. Standing the four bottles 1# and 2# and the four bottles 3# and 4# to simulate the release condition under the still water condition; the three bottles # 5, # 6 and # 7 are magnetically stirred and the stirring speed is controlled, so that the release condition of the microbial inoculum under the action of water flow is simulated. After stopping the stirrer every day, the condition of the tablets was photographed and recorded, and registration and photo preservation were made. The dissolved oxygen concentration in the water in the four bottles # 3, # 4, # 6 and # 7 was measured daily using a dissolved oxygen meter, then the water in the conical flask was poured out, the tablets were left, 500mL of fresh deionized water was replaced, and after oxygen was purged with argon until the dissolved oxygen was reduced to 0mg/L, the bottles were resealed. Calculating the oxygen release condition of the medicament; after evenly mixing the water samples 5-10 mL from the bottles 1#, 2# and 5# every 5 days, diluting, coating the flat plate, then placing the flat plate into an incubator for culture at 37 ℃, and observing the bacterial growth every day.
The embodiment shows that the prepared microbial inoculum can keep a good and complete form within 15 days under the action of still water and running water, the release time of dissolved oxygen can reach more than 10 days, and the release rate of oxygen can reach 1.3-5.7 and 5.7mgO 2 /(g microbial inoculum day).
Example six: the application of the nitrate type slow-release composite microbial inoculum in black and odorous water treatment is shown in fig. 4, and specifically comprises the following steps:
step S1, preparation of a microbial inoculum: according to the manufacturing scheme of the first embodiment, the finished product of the flaky nitrate type slow-release composite microbial agent is obtained.
Step S2, purifying black and odorous water body: black and odorous water is retrieved from a rural black and odorous water body in the turnip city, the raw water quality is COD=221 mg/L, turbidity=115 and ammonia nitrogen=14 mg/L, and the raw water is packaged in different reactors with total volumes of 80L to develop simulation experiments. Then adding nitrate type slow-release compound microbial agent into the reactor according to 1kg microbial agent per cubic meter, stirring and culturing at room temperature, continuously culturing, and periodically monitoring the water quality change condition. Finally, after ten days, the COD, turbidity and ammonia nitrogen removal rate of the black and odorous water reach 80%, 76% and 65% respectively; after the water quality reaches 45 days, the water quality can meet the water quality requirement of 4 types of water in the surface water environment quality standard (GB 3838-2002).
Embodiment seven: the application of the peroxide type slow-release composite microbial inoculum in black and odorous water treatment is specifically as follows:
step S1, preparation of a microbial inoculum: according to the manufacturing scheme of the second embodiment, the sheet-shaped peroxide type slow-release composite microbial agent finished product is obtained.
Step S2, purifying black and odorous water body: black and odorous water is retrieved from a rural black and odorous water body in the turnip city, the raw water quality is COD=187 mg/L, turbidity=83 and ammonia nitrogen=37 mg/L, and the raw water is packaged in different reactors with total volumes of 80L to develop simulation experiments. And then adding the peroxide type slow-release compound microbial agent into the reactor according to 1kg of microbial agent per cubic meter, stirring and culturing at room temperature, continuously culturing, and periodically monitoring the water quality change condition. Finally, after ten days, the COD, turbidity and ammonia nitrogen removal rate of the black and odorous water reach 89%, 67% and 84% respectively; after 25 days of operation, the water quality can meet the water quality requirement of 4 types of water in the surface water environment quality standard (GB 3838-2002).
Example eight: the comprehensive application of the nitrate-type and peroxide-type slow-release composite microbial inoculum in the treatment of black and odorous water is as follows:
step S1, according to the manufacturing scheme of the first embodiment and the second embodiment, a finished product of the tablet nitrate type and peroxy type slow-release compound microbial agent is obtained.
S2, preparing and split charging a high-concentration black and odorous water body: retrieving black and odorous water from a rural black and odorous water body in the Ulva city, adding nutritional substances such as starch, urea and ferrous sulfate, culturing in a water bath at 30 ℃ for 10 days to obtain high-concentration black and odorous water, wherein the water quality is COD=298 mg/L, turbidity=251, ammonia nitrogen=56 mg/L, total nitrogen=64 mg/L, and performing simulation experiments in different reactors with total volumes of 80L.
Step S3, using a peroxy type slow-release bacterial agent: adding a peroxide type slow-release compound microbial agent into the reactor according to 0.5kg microbial agent per cubic meter, stirring and culturing at room temperature, continuously culturing, and periodically monitoring the water quality change condition. Finally, after 15 days, the removal rates of COD, turbidity, ammonia nitrogen and total nitrogen of the black and odorous water reach 67%, 82%, 86% and 38% respectively, and the total nitrogen concentration is higher than that of the black and odorous water; and then adding a peroxide type slow-release compound microbial agent into the reactor according to 0.2kg microbial agent per cubic meter, stirring and culturing at room temperature, continuously culturing, and periodically monitoring the water quality change condition. After 10 days, the removal rates of COD, turbidity, ammonia nitrogen and total nitrogen of the black and odorous water reach more than 89%, 93%, 95% and 81% respectively.
In summary, by means of the technical scheme, the preparation method of the composite microbial agent is simple, raw materials are cheap and easy to obtain, the composite microbial agent is environment-friendly and suitable for large-scale production, and the aim of slowly releasing electron acceptors such as oxygen, nitrate and the like can be fulfilled; meanwhile, in the black and odorous water treatment process, the mixing proportion and the adding sequence can be reasonably selected according to the pollution condition of the black and odorous water, so that the requirements on purifying and ecological restoration of different black and odorous water bodies are met, and the black and odorous water treatment method has wide application prospects in the field of water environment treatment.
The foregoing is merely a preferred embodiment of the present invention and is not intended to limit the present invention, and other embodiments of the present disclosure will be readily apparent to those skilled in the art after considering the disclosure herein in the specification and examples. This application is intended to cover any adaptations, uses, or adaptations of the disclosure following, in general, the principles of the disclosure and including such departures from the present disclosure as come within known or customary practice within the art to which the disclosure pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the disclosure being indicated by the following claims.
It is to be understood that the present disclosure is not limited to the precise arrangements and instrumentalities shown in the drawings, and that various modifications and changes may be effected without departing from the scope thereof. The scope of the present disclosure is limited only by the appended claims.
Claims (10)
1. The slow-release type composite microbial agent is characterized by comprising the following components in parts by weight: 2-20 parts of dry material mixture and 1 part of slow release agent, wherein;
the dry material mixture consists of the following components: 1 part of mixed bacterial powder and 2 to 5 parts of auxiliary materials.
2. The slow-release composite microbial agent according to claim 1, wherein the mixed microbial powder consists of the following components: 4-8 parts of bacillus powder, 1-3 parts of nitrifying bacteria powder and 1-3 parts of denitrifying bacteria powder.
3. The slow-release composite microbial agent according to claim 1 or 2, wherein the auxiliary material consists of the following components: 2 to 6 parts of electron acceptor, 1 to 4 parts of coagulant and 0.3 part of microorganism promoter.
4. The slow-release composite microbial agent according to claim 3, wherein the electron acceptor is any one of calcium nitrate, calcium peroxide and sodium percarbonate.
5. The slow-release composite microbial agent according to claim 4, wherein the coagulant consists of the following components: 2-1 parts of polyacrylamide and 1 part of polyaluminum chloride.
6. The slow release type composite microbial agent according to claim 5, wherein the microbial promoter consists of the following components: 1 part of cellulose B, 1 part of cysteine and 1 part of sodium dihydrogen phosphate.
7. The sustained-release composite microbial agent according to claim 1 or 6, wherein the sustained-release agent is polylactic acid.
8. A method for preparing a slow-release type composite microbial agent, which is used for the preparation method of the slow-release type composite microbial agent as claimed in any one of claims 1 to 7, and is characterized by comprising the following steps:
preparing a bacterial powder mixture in advance, and respectively inoculating strains of bacillus, nitrifying bacteria and denitrifying bacteria into a culture solution; then adding 5-30 g of microorganism carrier sterilized for 30min at 121 ℃ per 100mL of culture solution, and continuing to culture for 2-5 days to enable microorganisms to adhere to the carrier; obtaining a carrier mixture to which the strain is attached; flooding the obtained carrier mixture with a solution containing 5-15% of skimmed milk powder for freezing and icing; freeze-drying the frozen carrier mixture to obtain bacterial powder of three strains respectively; uniformly mixing the dried bacterial powder of the three strains of bacteria to obtain a bacterial powder mixture;
preparing auxiliary materials, adding an electron acceptor, a coagulant and a microbial accelerator into a grinder, and fully grinding and uniformly mixing to obtain the auxiliary materials;
preparing a dry material mixture, and respectively mixing the prepared fungus powder mixture and auxiliary materials, and fully and uniformly stirring to obtain the dry material mixture;
preparing a slow release agent solution, adding the slow release agent into dichloromethane, soaking for 1-3 hours, and carrying out cold water ultrasonic treatment to obtain the slow release agent solution;
and (3) preparing the composite microbial agent, and pouring the prepared dry material mixture into a slow-release agent solution to be fully and uniformly stirred and mixed to obtain the composite microbial agent.
9. The method for preparing a slow-release composite microbial agent according to claim 8, wherein the step of preparing the composite microbial agent further comprises the steps of:
molding the composite microbial agent, and adding the composite microbial agent into a tablet press or a granulator to be pressed into tablets or granulated to obtain a tablet or granular slow-release type crude product of the composite microbial agent;
and drying the crude product of the composite microbial agent for 7-10 hours at the room temperature of 35+/-2 ℃ or in an oven to obtain a sheet or granular slow-release composite microbial agent finished product.
10. The application of the slow-release compound microbial agent in treating black and odorous water bodies is characterized by comprising the following steps:
and throwing the prepared flaky or granular slow-release composite microbial agent finished product into black and odorous water to be treated, and detecting the water quality periodically.
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