CN106667868A - Preparation method of skin-care type disinfection and sterilization hand sanitizer - Google Patents

Abstract

The invention relates to a preparation method of a skin-care type disinfection and sterilization hand sanitizer, and belongs to the technical field of hand sanitizers. The preparation method comprises the following steps of firstly, by taking banana peel and lemon peel as fermenting substrates, fermenting by probiotics to obtain enzyme, wherein the banana peel contains banana peel element to inhibit bacteria and fungi breeding, and prevent and treat rhagadia and chilblain, and the enzyme contains rich vitamins, enzyme, secondary metabolites and other nutrition elements to resist aging and bacteria, diminish inflammation, and the like; extracting phosvitin from duck eggs, wherein the phosvitin has excellent moisturizing property and nutrition, and can nourish hand skin; extracting tea saponin from tea oil meal, and mixing, so as to obtain the skin-care type disinfection and sterilization hand sanitizer. The prepared skin-care type disinfection and sterilization hand sanitizer has the advantages that the problems of irritation and toxicity to skin due to use of a sterilizer are solved, and the excellent effect of simultaneously nourishing skin and sterilizing is realized; the toxicity is avoided, the bacteria-inhibiting property is strong, the volatility is good, the stability is good, and the skin-care effect is realized.

Description

A kind of preparation method of skin-care disinfecting and sterilizing hand sanitizer

Technical field

The present invention relates to a kind of preparation method of skin-care disinfecting and sterilizing hand sanitizer, belongs to handwashing liquid technical field.

Background technology

Epidemiology finds that most of infectious disease can be used as route of transmission by handss with lemology research, cures in addition The handss of institute medical worker are the major reasons for causing nosocomial infection, thus using rational hand-wrist bones be control hospital infection and The important method of infectious disease transmission.

People's hand-washing article in the past is mainly soap, fancy soap etc., easily slides when they are used, and meets water logging, high temperature again Can soften and expend naturally, especially easily be infected with dirt, antibacterial on hand, become pollution source.With carrying for living standard The transformation of high and consumption idea, handwashing liquid has been increasingly becoming the succedaneum of traditional hand-washing article, at present oneself be widely used in hospital, The various places such as hotel, food and drink, family.The contact surface typically just bottle pump head of handwashing liquid and handss, it is easy to clean, it is to avoid hand over Fork infection.By squash type taken amount, nozzle need to be only gently pressed, liquid just flows into the palm of the hand, and consumption is grasped by oneself, and storage is used It is quite convenient to.

Nowadays, the maturation with handwashing liquid production technology and the popularization in market, special with various special efficacies is washed one's hands Liquid also constantly comes out.Such as heavy duty detergent handwashing liquid, paint-removing handwashing liquid, anhydrous washing hand liquid, disinfection handwashing liquid.Its In, disinfection handwashing liquid is the maximum class of occupancy volume in the special handwashing liquid in market.

It is reported that, the disinfection handwashing liquid for commonly using currently on the market, the effect in terms of disinfection compares reason Think.But not good enough from the aspect of the nursing to hand skin, the component of disinfection is larger to the zest of skin, life-time service Easily cause hand skin drying, coarse, religion to split and peel.Therefore, for the research of skin-care disinfecting and sterilizing hand sanitizer, it appears It is particularly important.

The content of the invention

The invention mainly solves the technical problem of：It is poor for traditional handwashing liquid sterilization effect, in use Larger to the zest of skin, life-time service easily causes the problem that hand skin is dried, coarse, religion is split and peeled, there is provided a kind of Ferment is prepared as fermenting raw materials with Pericarpium Musae and Fericarpium Citri Limoniae, then phosvitin is extracted from Ovum Anas domestica yolk, with the use of offer Skin care ingredient, recycles plant skin element to provide sterilizing composition to prepare the side of skin-care disinfecting and sterilizing hand sanitizer with tea saponin Method, the present invention first with Fericarpium Citri Limoniae smashs Pericarpium Musae to pieces, then obtains as fermentation substrate, Jing probioticss fermentation with brown sugar, sterilized water To ferment, plant skin element is contained in Pericarpium Musae, antibacterial and funguses can be suppressed to grow, while chap, chilblain can be prevented and treated, contained in ferment The nutrients such as abundant vitamin, enzyme and secondary metabolite, with the effect such as defying age, anti-inflammation, then from Ovum Anas domestica Phosvitin is extracted, phosvitin has excellent moisture retention and nutritional labeling, can moisten hand skin, Ran Houzai Tea saponin is extracted from the Oleum Camelliae dregs of rice, is mixed with and is obtained skin-care disinfecting and sterilizing hand sanitizer, skin-protection type sterilization prepared by the present invention Disinfection hand cleanser overcomes the problem for producing stimulation and toxicity to skin using antibacterial, reaches and improve simultaneously skin protection and sterilization work Excellent effect, has the advantages that nontoxic, bactericide bacteriostasis are strong, volatility is good, good stability and skin protection.

In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is：

（1）The fresh Pericarpium Musae of 400~500g and 20~30g Fresh Lemon skins are taken, after cleaning 2~4 times with water, drug ramming pot is placed in and is smash Broken 35~40min, by the material after smashing to pieces 30~40min of sterilization under ultraviolet germicidal lamp is placed in, then presses matter with brown sugar, sterilized water Amount compares 5:2:3, in being added to fermentation tank, stirring 3~5min of mixing obtains fermentation substrate；

（2）The yeast powder and Lactobacillus species for taking above-mentioned fermentation substrate quality 5~8%, 3~5% respectively is added to sterilizing beaker In, the sterilized water of 100~200mL35~45 DEG C is added, stirring 10~15min of mixing obtains mixed vaccine solution, then will mixing To on fermentation substrate, it is 120~150r/min to control fermentation jar temperature for 35~40 DEG C, speed of agitator to bacterium solution inoculum, is sealed Fermentation 8~9 days, fermentation ends collect the tunning in fermentation tank, in being placed in centrifuge, with 4000~5000r/min's Rotating speed is centrifuged 15~20min, collects upper liquid, i.e. ferment, standby；

（3）Ovum Anas domestica is taken, and is manually separated Ovum Gallus domesticus album with egg yolk, collect egg yolk, weighed 200~300g egg yolk and be added to and fill 400~500mL mass fractions are that in the beaker of 15% sodium chloride solution, stirring is extracted after 2~3h, in being transferred to centrifuge, with The rotating speed of 4000~5000r/min is centrifuged 20~25min, collects upper liquid, and is added in beaker, and it is heated to Boiling, after 20~30min of isothermal holding, pouring in buchner funnel carries out sucking filtration, collects filtrate, standby；

（4）300~400g Oleum Camelliae dregs of rice are weighed, is added in pulverizer and is crushed after 30~40min, cross 80~100 mesh sieves, collected Sieve granule, and be added in the beaker for filling 600~700mL water, it is carried out to be heated to boiling, 1~2h is incubated, stop adding Heat, after temperature naturally cools to 40~50 DEG C, adds 1~3g papains, stirring to digest 2~3h, and enzymolysis terminates, to enzyme Add 200~300mL mass fractions to be 95% ethanol solution in solution liquid, filter after 40~50min of stirring, collect filtrate, be placed in In Rotary Evaporators, the 1/6 of rotary evaporation to original volume, concentrated solution is obtained, 200~300mL ether is added in concentrated solution, stir Mix and filtered after 20~30min of mixing, collect filtering residue, i.e. tea saponin；

（5）Count by weight, weigh 30~40 parts of steps（2）Standby ferment, 20~30 parts of steps（3）Standby filtrate, 15~20 parts of above-mentioned tea saponins, 3~5 parts of shitosans, 3~5 parts of sodium bicarbonate and 20~30 parts of water, in being added to blender, stir Mix 5~10min of mixing, you can obtain skin-care disinfecting and sterilizing hand sanitizer.

The application process of the present invention：First both hands are sufficiently humidified so as to clear water, then take the obtained skin-protection type of 0.8~1.2g present invention Disinfection handwashing liquid is placed at the both hands of the centre of the palm, is rubbed and rinsed well both hands with clear water after 25~35s, subsequently doing with cleaning Towel.After testing, the present invention prepares the moderate performance of handwashing liquid, does not stimulate skin, puts in 2~5 DEG C of refrigerator 24h is put, is taken out and is recovered to during room temperature to observe, not stratified, without precipitation, product is not transparent muddy, and the obtained skin-protection type of the present invention The bacteriostasis rate of disinfection handwashing liquid is 92~96%, and sterilizing rate is more than 99.90%.

The invention has the beneficial effects as follows：

（1）Skin-care disinfecting and sterilizing hand sanitizer prepared by the present invention has that nontoxic, volatility is good, stability is good and skin protection excellent Point；

（2）Skin-care disinfecting and sterilizing hand sanitizer prepared by the present invention overcomes to produce skin using antibacterial to stimulate and toxicity Problem, reaches the excellent effect for improving skin protection and bactericidal action simultaneously.

Specific embodiment

The fresh Pericarpium Musae of 400~500g and 20~30g Fresh Lemon skins are taken, after cleaning 2~4 times with water, drug ramming pot is placed in Smash 35~40min to pieces, the material after smashing to pieces is placed in into 30~40min of sterilization under ultraviolet germicidal lamp, then press with brown sugar, sterilized water Mass ratio 5:2:3, in being added to fermentation tank, stirring 3~5min of mixing obtains fermentation substrate；Above-mentioned fermentation substrate matter is taken respectively The yeast powder and Lactobacillus species of amount 5~8%, 3~5% is added in sterilizing beaker, adds 100~200mL35~45 DEG C Sterilized water, stirring 10~15min of mixing obtains mixed vaccine solution, then by mixed vaccine solution inoculum to fermentation substrate, control Fermentation jar temperature is 35~40 DEG C, speed of agitator is 120~150r/min, and sealing and fermenting 8~9 days, fermentation ends collect fermentation Tunning in tank, in being placed in centrifuge, with the rotating speed of 4000~5000r/min 15~20min is centrifuged, and collects upper strata Liquid, i.e. ferment, take Ovum Anas domestica, and are manually separated Ovum Gallus domesticus album with egg yolk, collect egg yolk, weigh 200~300g egg yolk and are added to In filling the beaker that 400~500mL mass fractions are 15% sodium chloride solution, stirring is extracted after 2~3h, in being transferred to centrifuge, 20~25min is centrifuged with the rotating speed of 4000~5000r/min, upper liquid is collected, and is added in beaker, and it is heated To seething with excitement, after 20~30min of isothermal holding, pouring in buchner funnel carries out sucking filtration, collects filtrate, weighs 300~400g Oleum Camelliae The dregs of rice, are added in pulverizer and crush after 30~40min, cross 80~100 mesh sieves, and collection is sieved granule, and be added to fill 600~ In the beaker of 700mL water, it is carried out to be heated to boiling, be incubated 1~2h, stop heating, treat that temperature naturally cools to 40~50 After DEG C, add 1~3g papains, stirring 2~3h of enzymolysis, enzymolysis to terminate, 200~300mL mass is added in enzymolysis solution Fraction is 95% ethanol solution, is filtered after 40~50min of stirring, collects filtrate, and in being placed in Rotary Evaporators, rotary evaporation is extremely The 1/6 of original volume, obtains concentrated solution, and 200~300mL ether is added in concentrated solution, filters after stirring 20~30min of mixing, Collect filtering residue, i.e. tea saponin；Count by weight, weigh 30~40 parts of ferment, 20~30 parts of filtrates, 15~20 parts of above-mentioned tea soaps Element, 3~5 parts of shitosans, 3~5 parts of sodium bicarbonate and 20~30 parts of water, in being added to blender, stirring 5~10min of mixing, i.e., Skin-care disinfecting and sterilizing hand sanitizer is obtained.

Example 1

The fresh Pericarpium Musae of 400g and 20g Fresh Lemon skins are taken, after cleaning 2 times with water, drug ramming pot is placed in and is smashed 35min to pieces, will be smashed to pieces Material afterwards is placed under ultraviolet germicidal lamp the 30min that sterilizes, then with brown sugar, sterilized water in mass ratio 5:2:3, it is added to fermentation tank In, stirring mixing 3min obtains fermentation substrate；Yeast powder and the lactobacilluss bacterium of above-mentioned fermentation substrate quality 5%, 3% are taken respectively Plant and be added in sterilizing beaker, add 100mL35 DEG C of sterilized water, stirring mixing 10min obtains mixed vaccine solution, then will Mixed vaccine solution inoculum on fermentation substrate, control fermentation jar temperature for 35 DEG C, speed of agitator be 120r/min, sealing and fermenting 8 My god, fermentation ends collect the tunning in fermentation tank, in being placed in centrifuge, 15min are centrifuged with the rotating speed of 4000r/min, Upper liquid, i.e. ferment are collected, Ovum Anas domestica is taken, and is manually separated Ovum Gallus domesticus album with egg yolk, collect egg yolk, weigh the addition of 200g egg yolk To filling in the beaker that 400mL mass fractions are 15% sodium chloride solution, stirring is extracted after 2h, in being transferred to centrifuge, with The rotating speed centrifugation 20min of 4000r/min, collects upper liquid, and is added in beaker, and it is carried out to be heated to boiling, insulation After processing 20min, pouring in buchner funnel carries out sucking filtration, collects filtrate, weighs the 300g Oleum Camelliae dregs of rice, is added in pulverizer and crushes After 30min, 80 mesh sieves are crossed, collect the granule that sieves, and be added in the beaker for filling 600mL water, it is carried out to be heated to boiling, Insulation 1h, stops heating, after temperature naturally cools to 40 DEG C, adds 1g papains, stirring enzymolysis 2h, enzymolysis to terminate, Add 200mL mass fractions to be 95% ethanol solution in enzymolysis solution, filter after stirring 40min, collect filtrate, be placed in rotation In evaporimeter, the 1/6 of rotary evaporation to original volume, concentrated solution is obtained, 200mL ether, stirring mixing are added in concentrated solution Filter after 20min, collect filtering residue, i.e. tea saponin；Count by weight, weigh 30 parts of ferment, 20 parts of filtrates, 15 parts of above-mentioned tea soaps Element, 3 parts of shitosans, 3 parts of sodium bicarbonate and 20 parts of water, in being added to blender, stirring mixing 5min, you can obtain skin-protection type and disappear Malicious disinfection hand cleanser.

First both hands are sufficiently humidified so as to clear water, then skin-care disinfecting and sterilizing hand sanitizer obtained in taking the 0.8g present invention is placed in the centre of the palm At both hands, rub and rinsed well both hands with clear water after 25s, subsequently dried with the dry towel of cleaning.After testing, Jing inspections Survey, the present invention prepares the moderate performance of handwashing liquid, does not stimulate skin, 24h is placed in 2 DEG C of refrigerator, take out and recover to room temperature When observe, not stratified, without precipitation, product is not transparent muddy, and the obtained skin-care disinfecting and sterilizing hand sanitizer of the present invention is antibacterial Rate is 92%, and sterilizing rate is 99.91%.

Example 2

The fresh Pericarpium Musae of 500g and 30g Fresh Lemon skins are taken, after cleaning 4 times with water, drug ramming pot is placed in and is smashed 40min to pieces, will be smashed to pieces Material afterwards is placed under ultraviolet germicidal lamp the 40min that sterilizes, then with brown sugar, sterilized water in mass ratio 5:2:3, it is added to fermentation tank In, stirring mixing 5min obtains fermentation substrate；Yeast powder and the lactobacilluss bacterium of above-mentioned fermentation substrate quality 8%, 5% are taken respectively Plant and be added in sterilizing beaker, add 200mL45 DEG C of sterilized water, stirring mixing 15min obtains mixed vaccine solution, then will Mixed vaccine solution inoculum on fermentation substrate, control fermentation jar temperature for 40 DEG C, speed of agitator be 150r/min, sealing and fermenting 9 My god, fermentation ends collect the tunning in fermentation tank, in being placed in centrifuge, 20min are centrifuged with the rotating speed of 5000r/min, Upper liquid, i.e. ferment are collected, Ovum Anas domestica is taken, and is manually separated Ovum Gallus domesticus album with egg yolk, collect egg yolk, weigh the addition of 300g egg yolk To filling in the beaker that 500mL mass fractions are 15% sodium chloride solution, stirring is extracted after 3h, in being transferred to centrifuge, with The rotating speed centrifugation 25min of 5000r/min, collects upper liquid, and is added in beaker, and it is carried out to be heated to boiling, insulation After processing 30min, pouring in buchner funnel carries out sucking filtration, collects filtrate, weighs the 400g Oleum Camelliae dregs of rice, is added in pulverizer and crushes After 40min, 100 mesh sieves are crossed, collect the granule that sieves, and be added in the beaker for filling 700mL water, it is carried out to be heated to boiling Rise, be incubated 2h, stop heating, after temperature naturally cools to 50 DEG C, add 3g papains, stirring enzymolysis 3h, enzymolysis knot Beam, adds 300mL mass fractions to be 95% ethanol solution in enzymolysis solution, filters after stirring 50min, collects filtrate, is placed in rotation In turning evaporimeter, the 1/6 of rotary evaporation to original volume, concentrated solution is obtained, 300mL ether, stirring mixing are added in concentrated solution Filter after 30min, collect filtering residue, i.e. tea saponin；Count by weight, weigh 40 parts of ferment, 30 parts of filtrates, 20 parts of above-mentioned tea soaps Element, 5 parts of shitosans, 4 parts of sodium bicarbonate and 30 parts of water, in being added to blender, stirring mixing 10min, you can obtain skin-protection type Disinfection handwashing liquid.

First both hands are sufficiently humidified so as to clear water, then skin-care disinfecting and sterilizing hand sanitizer obtained in taking the 1.2g present invention is placed in the centre of the palm At both hands, rub and rinsed well both hands with clear water after 35s, subsequently dried with the dry towel of cleaning.After testing, the present invention The moderate performance of handwashing liquid is prepared, does not stimulate skin, 24h is placed in 5 DEG C of refrigerator, taken out and recover to during room temperature to observe, no Layering, without precipitation, product is not transparent muddy, and the bacteriostasis rate of the obtained skin-care disinfecting and sterilizing hand sanitizer of the present invention is 96%, is killed Bacterium rate is 99.93%.

Example 3

The fresh Pericarpium Musae of 450g and 25g Fresh Lemon skins are taken, after cleaning 3 times with water, drug ramming pot is placed in and is smashed 38min to pieces, will be smashed to pieces Material afterwards is placed under ultraviolet germicidal lamp the 35min that sterilizes, then with brown sugar, sterilized water in mass ratio 5:2:3, it is added to fermentation tank In, stirring mixing 4min obtains fermentation substrate；Yeast powder and the lactobacilluss bacterium of above-mentioned fermentation substrate quality 7%, 4% are taken respectively Plant and be added in sterilizing beaker, add 150mL40 DEG C of sterilized water, stirring mixing 13min obtains mixed vaccine solution, then will Mixed vaccine solution inoculum on fermentation substrate, control fermentation jar temperature for 38 DEG C, speed of agitator be 135r/min, sealing and fermenting 9 My god, fermentation ends collect the tunning in fermentation tank, in being placed in centrifuge, 18min are centrifuged with the rotating speed of 4500r/min, Upper liquid, i.e. ferment are collected, Ovum Anas domestica is taken, and is manually separated Ovum Gallus domesticus album with egg yolk, collect egg yolk, weigh the addition of 250g egg yolk To filling in the beaker that 450mL mass fractions are 15% sodium chloride solution, stirring is extracted after 3h, in being transferred to centrifuge, with The rotating speed centrifugation 23min of 4500r/min, collects upper liquid, and is added in beaker, and it is carried out to be heated to boiling, insulation After processing 25min, pouring in buchner funnel carries out sucking filtration, collects filtrate, weighs the 350g Oleum Camelliae dregs of rice, is added in pulverizer and crushes After 35min, 90 mesh sieves are crossed, collect the granule that sieves, and be added in the beaker for filling 650mL water, it is carried out to be heated to boiling, Insulation 2h, stops heating, after temperature naturally cools to 45 DEG C, adds 2g papains, stirring enzymolysis 3h, enzymolysis to terminate, Add 250mL mass fractions to be 95% ethanol solution in enzymolysis solution, filter after stirring 45min, collect filtrate, be placed in rotation In evaporimeter, the 1/6 of rotary evaporation to original volume, concentrated solution is obtained, 250mL ether, stirring mixing are added in concentrated solution Filter after 25min, collect filtering residue, i.e. tea saponin；Count by weight, weigh 35 parts of ferment, 25 parts of filtrates, 18 parts of above-mentioned tea soaps Element, 4 parts of shitosans, 5 parts of sodium bicarbonate and 25 parts of water, in being added to blender, stirring mixing 8min, you can obtain skin-protection type and disappear Malicious disinfection hand cleanser.

First both hands are sufficiently humidified so as to clear water, then skin-care disinfecting and sterilizing hand sanitizer obtained in taking the 1g present invention is placed in the centre of the palm pair At handss, rub and rinsed well both hands with clear water after 30s, subsequently dried with the dry towel of cleaning.After testing, after testing, The present invention prepares the moderate performance of handwashing liquid, does not stimulate skin, and 24h is placed in 3 DEG C of refrigerator, takes out and recovers to during room temperature to see Examine, not stratified, without precipitation, product is not transparent muddy, and the bacteriostasis rate of the obtained skin-care disinfecting and sterilizing hand sanitizer of the present invention is 94%, sterilizing rate is 99.92%.

Claims (1)

1. a kind of preparation method of skin-care disinfecting and sterilizing hand sanitizer, it is characterised in that concrete preparation process is：

（1）The fresh Pericarpium Musae of 400~500g and 20~30g Fresh Lemon skins are taken, after cleaning 2~4 times with water, drug ramming pot is placed in and is smash Broken 35~40min, by the material after smashing to pieces 30~40min of sterilization under ultraviolet germicidal lamp is placed in, then presses matter with brown sugar, sterilized water Amount compares 5:2:3, in being added to fermentation tank, stirring 3~5min of mixing obtains fermentation substrate；

（2）The yeast powder and Lactobacillus species for taking above-mentioned fermentation substrate quality 5~8%, 3~5% respectively is added to sterilizing beaker In, the sterilized water that 100~200mL temperature is 35~45 DEG C is added, stirring 10~15min of mixing obtains mixed vaccine solution, then Mixed vaccine solution is added in fermentation tank, it is 120~150r/min to control fermentation jar temperature for 35~40 DEG C, speed of agitator, Sealing and fermenting 8~9 days, fermentation ends collect the tunning in fermentation tank, in being placed in centrifuge, with 4000~5000r/ The rotating speed of min is centrifuged 15~20min, collects upper liquid, i.e. ferment, standby；

（3）Ovum Anas domestica is taken, and is manually separated Ovum Gallus domesticus album with egg yolk, collect egg yolk, weighed 200~300g egg yolk and be added to and fill 400~500mL mass fractions are that in the beaker of 15% sodium chloride solution, stirring is extracted after 2~3h, in being transferred to centrifuge, with The rotating speed of 4000~5000r/min is centrifuged 20~25min, collects upper liquid, and is added in beaker, and it is heated to Boiling, after 20~30min of isothermal holding, pouring in buchner funnel carries out sucking filtration, collects filtrate, standby；

（4）300~400g Oleum Camelliae dregs of rice are weighed, is added in pulverizer and is crushed after 30~40min, cross 80~100 mesh sieves, collected Sieve granule, and be added in the beaker for filling 600~700mL water, it is carried out to be heated to boiling, 1~2h is incubated, stop adding Heat, after temperature naturally cools to 40~50 DEG C, adds 1~3g papains, stirring to digest 2~3h, and enzymolysis terminates, to enzyme Add 200~300mL mass fractions to be 95% ethanol solution in solution liquid, filter after 40~50min of stirring, collect filtrate, be placed in In Rotary Evaporators, the 1/6 of rotary evaporation to original volume, concentrated solution is obtained, 200~300mL ether is added in concentrated solution, stir Mix and filtered after 20~30min of mixing, collect filtering residue, i.e. tea saponin；

（5）Count by weight, weigh 30~40 parts of steps（2）Standby ferment, 20~30 parts of steps（3）Standby filtrate, 15~20 parts of above-mentioned tea saponins, 3~5 parts of shitosans, 3~5 parts of sodium bicarbonate and 20~30 parts of water, in being added to blender, stir Mix 5~10min of mixing, you can obtain skin-care disinfecting and sterilizing hand sanitizer.