CN106662584B - 分离靶细胞的方法 - Google Patents

分离靶细胞的方法 Download PDF

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Publication number
CN106662584B
CN106662584B CN201580035819.4A CN201580035819A CN106662584B CN 106662584 B CN106662584 B CN 106662584B CN 201580035819 A CN201580035819 A CN 201580035819A CN 106662584 B CN106662584 B CN 106662584B
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binding
ligand
streptavidin
reagent
receptor molecule
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CN106662584A (zh
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U·D·卡尔
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IBA Lifesciences GmbH
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IBA GmbH
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580035819.4A 2014-04-30 2015-04-30 分离靶细胞的方法 Active CN106662584B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14166718.8 2014-04-30
EP14166718 2014-04-30
PCT/EP2015/059510 WO2015166049A1 (en) 2014-04-30 2015-04-30 Method of isolating a target cell

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CN106662584A CN106662584A (zh) 2017-05-10
CN106662584B true CN106662584B (zh) 2020-05-05

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US (1) US11150239B2 (enExample)
EP (1) EP3137905B1 (enExample)
JP (1) JP6678593B2 (enExample)
CN (1) CN106662584B (enExample)
AU (1) AU2015254588B2 (enExample)
CA (1) CA2945414C (enExample)
WO (1) WO2015166049A1 (enExample)

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SG11201404991YA (en) 2012-02-23 2014-09-26 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials
SG11201608557UA (en) * 2014-04-16 2016-11-29 Juno Therapeutics Gmbh Methods, kits and apparatus for expanding a population of cells
KR20180108567A (ko) 2015-10-22 2018-10-04 주노 테라퓨틱스 게엠베하 형질도입을 위한 방법, 키트, 제제 및 장치
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
MA45488A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés, kits et appareil de culture de cellules
IL270142B2 (en) 2017-04-27 2025-05-01 Juno Therapeutics Gmbh Oligomeric particle reagents and methods of use thereof
KR102088277B1 (ko) * 2018-04-27 2020-03-13 주식회사 유니언스진 면역 화학 진단법을 이용한 표적 항원 검출용 종이기반 3차원 구조의 미세칩과 이를 이용한 표적항원 검출 방법
CN112912103B (zh) * 2018-05-21 2025-08-29 广州邦定生物技术有限公司 多价蛋白质复合物
KR20210031927A (ko) * 2018-07-17 2021-03-23 유니버시티 오브 워싱톤 압타머 기반 가역적 세포 선택과 관련된 조성물 및 방법
CN112639434A (zh) * 2018-08-31 2021-04-09 热动力医疗公司 从血液中分离靶细胞的方法
AU2021213439B2 (en) * 2020-01-31 2023-01-05 Cell.Copedia GmbH Methods of isolating a biological entity
WO2023017115A1 (en) 2021-08-11 2023-02-16 Iba Lifesciences Gmbh Fractionation of cells based on a marker protein
WO2023209175A1 (en) 2022-04-28 2023-11-02 Bio-Recell Ltd. Method for separating target cells
WO2025238197A1 (en) * 2024-05-16 2025-11-20 Iba Lifesciences Gmbh Functionalized three-dimensional (3d) polymer scaffold for affinity chromatography

Citations (4)

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CN101622340A (zh) * 2006-11-15 2010-01-06 茵维特罗根戴纳股份公司 生物素化合物与支持物可逆结合的方法
WO2012044999A2 (en) * 2010-10-01 2012-04-05 Ludwig Institute For Cancer Research Ltd. Reversible protein multimers, methods for their production and use
WO2013011011A2 (en) * 2011-07-18 2013-01-24 Iba Gmbh Method of reversibly staining a target cell
WO2013124474A2 (en) * 2012-02-23 2013-08-29 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials

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WO1986002077A1 (en) 1984-10-02 1986-04-10 Meade Harry M Production of streptavidin-like polypeptides
US4851341A (en) 1986-12-19 1989-07-25 Immunex Corporation Immunoaffinity purification system
DE4237113B4 (de) 1992-11-03 2006-10-12 "Iba Gmbh" Peptide und deren Fusionsproteine, Expressionsvektor und Verfahren zur Herstellung eines Fusionsproteins
WO1996023879A1 (en) 1995-01-30 1996-08-08 Terrapin Technologies, Inc. Glubodies - multiplicities of proteins capable of binding a variety of small molecules
CA2211951A1 (en) 1995-02-09 1996-08-15 University Of Washington Modified-affinity streptavidin
AU5917796A (en) 1995-04-11 1997-04-09 Trustees Of Boston University Streptavidin mutants
DE19641876B4 (de) 1996-10-10 2011-09-29 Iba Gmbh Streptavidinmuteine
CA2283716A1 (en) 1997-03-14 1998-09-17 Gabriel O. Reznik Multiflavor streptavidin
US5985658A (en) 1997-11-14 1999-11-16 Health Research Incorporated Calmodulin-based cell separation technique
DE19932688B4 (de) 1999-07-13 2009-10-08 Scil Proteins Gmbh Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen
EP1227321A1 (en) 2000-12-28 2002-07-31 Institut für Bioanalytik GmbH Reversible MHC multimer staining for functional purification of antigen-specific T cells
DE10113776B4 (de) 2001-03-21 2012-08-09 "Iba Gmbh" Isoliertes streptavidinbindendes, kompetitiv eluierbares Peptid, dieses umfassendes Fusionspeptid, dafür codierende Nukleinsäure, Expressionsvektor, Verfahren zur Herstellung eines rekombinanten Fusionsproteins und Verfahren zum Nachweis und/oder zur Gewinnung des Fusionsproteins
WO2003029462A1 (en) 2001-09-27 2003-04-10 Pieris Proteolab Ag Muteins of human neutrophil gelatinase-associated lipocalin and related proteins
CN100554885C (zh) 2005-04-19 2009-10-28 松下电器产业株式会社 位置传感器、光头装置、头部移动机构及位置控制系统
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Patent Citations (4)

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CN101622340A (zh) * 2006-11-15 2010-01-06 茵维特罗根戴纳股份公司 生物素化合物与支持物可逆结合的方法
WO2012044999A2 (en) * 2010-10-01 2012-04-05 Ludwig Institute For Cancer Research Ltd. Reversible protein multimers, methods for their production and use
WO2013011011A2 (en) * 2011-07-18 2013-01-24 Iba Gmbh Method of reversibly staining a target cell
WO2013124474A2 (en) * 2012-02-23 2013-08-29 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials

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AU2015254588B2 (en) 2020-05-21
US11150239B2 (en) 2021-10-19
EP3137905A1 (en) 2017-03-08
US20170052176A1 (en) 2017-02-23
JP2017514481A (ja) 2017-06-08
JP6678593B2 (ja) 2020-04-08
WO2015166049A1 (en) 2015-11-05
CA2945414C (en) 2023-05-09
CA2945414A1 (en) 2015-11-05
AU2015254588A1 (en) 2016-11-10
CN106662584A (zh) 2017-05-10
EP3137905B1 (en) 2021-01-13

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