CN106661616A - 使用核酸卡尺的高通量结构测定 - Google Patents

使用核酸卡尺的高通量结构测定 Download PDF

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Publication number
CN106661616A
CN106661616A CN201580027026.8A CN201580027026A CN106661616A CN 106661616 A CN106661616 A CN 106661616A CN 201580027026 A CN201580027026 A CN 201580027026A CN 106661616 A CN106661616 A CN 106661616A
Authority
CN
China
Prior art keywords
target
nucleic acid
calliper rule
slide calliper
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201580027026.8A
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English (en)
Chinese (zh)
Inventor
W·M·施
W·P·翁
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston Childrens Hospital
Dana Farber Cancer Institute Inc
Original Assignee
Boston Childrens Hospital
Dana Farber Cancer Institute Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston Childrens Hospital, Dana Farber Cancer Institute Inc filed Critical Boston Childrens Hospital
Publication of CN106661616A publication Critical patent/CN106661616A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201580027026.8A 2014-04-23 2015-04-23 使用核酸卡尺的高通量结构测定 Pending CN106661616A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461982914P 2014-04-23 2014-04-23
US61/982,914 2014-04-23
PCT/US2015/027290 WO2015164602A2 (en) 2014-04-23 2015-04-23 High-throughput structure determination using nucleic acid calipers

Publications (1)

Publication Number Publication Date
CN106661616A true CN106661616A (zh) 2017-05-10

Family

ID=54333422

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580027026.8A Pending CN106661616A (zh) 2014-04-23 2015-04-23 使用核酸卡尺的高通量结构测定

Country Status (5)

Country Link
US (2) US9897597B2 (enExample)
EP (2) EP3134550B1 (enExample)
JP (1) JP2017514467A (enExample)
CN (1) CN106661616A (enExample)
WO (1) WO2015164602A2 (enExample)

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US8491454B2 (en) 2008-12-02 2013-07-23 President And Fellows Of Harvard College Spinning force apparatus
WO2015164602A2 (en) 2014-04-23 2015-10-29 Children's Medical Center Corporation High-throughput structure determination using nucleic acid calipers
WO2016089588A1 (en) 2014-12-06 2016-06-09 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
KR20160100175A (ko) * 2015-02-13 2016-08-23 주식회사 삼홍사 높낮이 조절이 가능한 책상
US11396650B2 (en) 2015-06-02 2022-07-26 Children's Medical Center Corporation Nucleic acid complexes for screening barcoded compounds
US12077807B2 (en) 2015-06-27 2024-09-03 The Research Foundation For The State University Of New York Compositions and methods for analyte detection using nanoswitches
US11713483B2 (en) 2016-02-09 2023-08-01 Children's Medical Center Corporation Method for detection of analytes via polymer complexes
JP2019512722A (ja) 2016-02-25 2019-05-16 ザ チルドレンズ メディカル センター コーポレーション 分子に関連した特徴の測定のためのスピン装置
WO2017151062A1 (en) * 2016-03-03 2017-09-08 National University Of Singapore A method of determining a presence of interaction between a substance molecule and a substrate molecule
WO2017165647A1 (en) 2016-03-23 2017-09-28 Children's Medical Center Corporation Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods
WO2017216270A1 (de) 2016-06-15 2017-12-21 Grabmayr Heinrich Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
TWM549567U (zh) * 2016-12-30 2017-10-01 Kelly Int Corp 薄型升降台
US11591636B2 (en) 2017-11-20 2023-02-28 Children's Medical Center Corporation Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids
CN109961144A (zh) * 2019-03-08 2019-07-02 华北电力大学 一种基于dna核酶的dna分子逻辑门
WO2021174071A1 (en) 2020-02-29 2021-09-02 President And Fellows Of Harvard College Nanoswitch caliper trains for high-throughput, high-resolution structural analysis of biomolecules
CN112998395A (zh) * 2021-04-22 2021-06-22 宁波翘楚智能设备科技有限公司 可升降桌上桌

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2012012037A1 (en) * 2010-07-19 2012-01-26 New England Biolabs, Inc. Oligonucleotide adaptors: compositions and methods of use

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US5023243A (en) 1981-10-23 1991-06-11 Molecular Biosystems, Inc. Oligonucleotide therapeutic agent and method of making same
ATE75483T1 (de) 1981-10-23 1992-05-15 Molecular Biosystems Inc Oligonukleotides heilmittel und dessen herstellungsverfahren.
WO1997023647A1 (en) * 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Homogeneous amplification and detection of nucleic acids
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
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US8491454B2 (en) 2008-12-02 2013-07-23 President And Fellows Of Harvard College Spinning force apparatus
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WO2012012037A1 (en) * 2010-07-19 2012-01-26 New England Biolabs, Inc. Oligonucleotide adaptors: compositions and methods of use

Non-Patent Citations (5)

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EMANUEL PFITZNER，ET AL: "Rigid DNA Beams for High-Resolution Single-Molecule Mechanics", 《ANGEWANDTE COMMUNICATIONS》 *
EMMANUEL BASLE´,ET AL: "Protein Chemical Modification on Endogenous Amino Acids", 《CHEMISTRY & BIOLOGY》 *
KIPOM KIM，ET AL: "A high-resolution magnetic tweezer for single-molecule measurements", 《NUCLEIC ACIDS RESEARCH》 *
MOUNIR A. KOUSSA，ET AL: "Protocol for sortase-mediated construction of DNA-protein hybrids and functional nanostructures", 《METHODS》 *

Also Published As

Publication number Publication date
US10914733B2 (en) 2021-02-09
JP2017514467A (ja) 2017-06-08
WO2015164602A3 (en) 2015-12-23
WO2015164602A2 (en) 2015-10-29
US20170045506A1 (en) 2017-02-16
EP3134550B1 (en) 2019-09-04
EP3134550A2 (en) 2017-03-01
US9897597B2 (en) 2018-02-20
EP3134550A4 (en) 2017-12-13
EP3594363A1 (en) 2020-01-15
US20180306783A1 (en) 2018-10-25

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