CN106635952B - 一种细胞复苏试剂 - Google Patents
一种细胞复苏试剂 Download PDFInfo
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- CN106635952B CN106635952B CN201610851439.4A CN201610851439A CN106635952B CN 106635952 B CN106635952 B CN 106635952B CN 201610851439 A CN201610851439 A CN 201610851439A CN 106635952 B CN106635952 B CN 106635952B
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N2500/00—Specific components of cell culture medium
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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Abstract
Description
克隆形成率 | |
第1组 | 5% |
第2组 | 10% |
第3组 | 50% |
第4组 | 75% |
第5组 | 90% |
第6组 | 60% |
第7组 | 65% |
第8组 | 50% |
第9组 | 45% |
细胞复苏率 | |
第1组 | 88% |
第2组 | 90% |
第3组 | 89% |
第4组 | 91% |
第5组 | 98% |
第6组 | 95% |
第7组 | 93% |
第8组 | 92% |
第9组 | 94% |
对照组 | 90% |
细胞名称 | 细胞特性 | 细胞复苏率 |
PBMC细胞 | 细胞形态正常 | 88% |
MSC细胞 | 细胞形态正常 | 97% |
CIK细胞 | 细胞形态正常 | 95% |
NK细胞 | 细胞形态正常 | 93% |
293细胞 | 细胞形态正常 | 99% |
HUVEC细胞 | 细胞形态正常 | 89% |
成纤维细胞 | 细胞形态正常 | 100% |
人ES细胞 | 细胞形态正常 | 86% |
人iPS细胞 | 细胞形态正常 | 88% |
Claims (1)
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CN201610851439.4A CN106635952B (zh) | 2016-09-26 | 2016-09-26 | 一种细胞复苏试剂 |
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CN201610851439.4A CN106635952B (zh) | 2016-09-26 | 2016-09-26 | 一种细胞复苏试剂 |
Publications (2)
Publication Number | Publication Date |
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CN106635952A CN106635952A (zh) | 2017-05-10 |
CN106635952B true CN106635952B (zh) | 2019-04-19 |
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CN201610851439.4A Active CN106635952B (zh) | 2016-09-26 | 2016-09-26 | 一种细胞复苏试剂 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107034195B (zh) * | 2017-05-18 | 2021-02-09 | 焕生汇生物基因技术(北京)有限公司 | 一种人类诱导性多能干细胞专用的复苏试剂及复苏方法 |
CN107164307A (zh) * | 2017-06-18 | 2017-09-15 | 广东博溪生物科技有限公司 | 一种细胞复苏方法 |
CN108330098A (zh) * | 2018-02-08 | 2018-07-27 | 安徽古生物科技有限公司 | 一种冻存细胞复苏的方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1412301A (zh) * | 2002-12-05 | 2003-04-23 | 中国科学院武汉病毒研究所 | 一种提高冻存细胞复苏率的细胞冻存液 |
CN1844371A (zh) * | 2005-04-08 | 2006-10-11 | 上海组织工程研究与开发中心 | 冻存胚胎干细胞的复苏方法 |
CN102228016A (zh) * | 2011-04-18 | 2011-11-02 | 上海安集协康生物技术有限公司 | 一种神经干细胞冻存复苏的方法 |
CN103404509A (zh) * | 2013-08-07 | 2013-11-27 | 彭乐 | 一种细胞保存液、其制备方法和应用 |
CN103798224A (zh) * | 2012-11-05 | 2014-05-21 | 中国人民解放军总医院第一附属医院 | 汗腺样细胞冻存复苏液及保持细胞活性的冻存复苏方法 |
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2016
- 2016-09-26 CN CN201610851439.4A patent/CN106635952B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1412301A (zh) * | 2002-12-05 | 2003-04-23 | 中国科学院武汉病毒研究所 | 一种提高冻存细胞复苏率的细胞冻存液 |
CN1844371A (zh) * | 2005-04-08 | 2006-10-11 | 上海组织工程研究与开发中心 | 冻存胚胎干细胞的复苏方法 |
CN102228016A (zh) * | 2011-04-18 | 2011-11-02 | 上海安集协康生物技术有限公司 | 一种神经干细胞冻存复苏的方法 |
CN103798224A (zh) * | 2012-11-05 | 2014-05-21 | 中国人民解放军总医院第一附属医院 | 汗腺样细胞冻存复苏液及保持细胞活性的冻存复苏方法 |
CN103404509A (zh) * | 2013-08-07 | 2013-11-27 | 彭乐 | 一种细胞保存液、其制备方法和应用 |
Non-Patent Citations (2)
Title |
---|
A549细胞冻存与复苏方法的改进;季晓宇 等;《中国科技信息》;20160701(第13期);第78-79页 |
The proliferation of human umbilical vein endothelial cells in serum-free medium;Philip G.de Groot 等;《Thrombosis Research》;19830815;第31卷(第4期);第623-634页 |
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Address after: Room 1006, 10th floor, No. 3 Courtyard, 30 Shixing Street, Shijingshan District, Beijing, 100041 Patentee after: Beijing huanshenghui Biotechnology Research Institute Co.,Ltd. Address before: Room 1002, 10th floor, 3rd building, 30 Shixing Street, Shijingshan District, Beijing Patentee before: BEIJING HUANSHENGHUI BIOLOGICAL TECHNOLOGY Research Institute |
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Effective date of registration: 20220307 Address after: Room 1006, 10th floor, No. 3 Courtyard, 30 Shixing Street, Shijingshan District, Beijing, 100041 Patentee after: Huanshenghui Biogenetic Technology (Beijing) Co.,Ltd. Address before: Room 1006, 10th floor, No. 3 Courtyard, 30 Shixing Street, Shijingshan District, Beijing, 100041 Patentee before: Beijing huanshenghui Biotechnology Research Institute Co.,Ltd. |