CN106631577A - Microbial bacterium and application thereof - Google Patents

Microbial bacterium and application thereof Download PDF

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Publication number
CN106631577A
CN106631577A CN201611118123.0A CN201611118123A CN106631577A CN 106631577 A CN106631577 A CN 106631577A CN 201611118123 A CN201611118123 A CN 201611118123A CN 106631577 A CN106631577 A CN 106631577A
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parts
bacillus
fermentation
fermentate
bacterial agent
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CN201611118123.0A
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罗增桂
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Nanning Guangxi Mao Mao Enterprise Information Consulting Co Ltd
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Nanning Guangxi Mao Mao Enterprise Information Consulting Co Ltd
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Priority to CN201611118123.0A priority Critical patent/CN106631577A/en
Publication of CN106631577A publication Critical patent/CN106631577A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • C05D9/02Other inorganic fertilisers containing trace elements
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/08Organic fertilisers containing added bacterial cultures, mycelia or the like
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Inorganic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a microbial bacterium, which is prepared from the following raw materials in parts by weight: mixed biological bacteria including 0.01 to 0.02 part of aspergillus foetidus, 0.02 to 0.04 part of aspergillus awamori, 0.01 to 0.02 part of bacillus mucilaginosus, 0.01 to 0.02 part of streptomyces roseoflavus, 0.01 to 0.02 part of bacillus benzoevorans, 0.03 to 0.05 part of bacillus megatherium and 0.03 to 0.05 part of bacillus polymyxa; other materials including 60 to 70 parts of sweet buckwheat, 10 to 12 parts of mangosteen peel powder, 3 to 5 parts of copper sulfate, 2 to 4 parts of ferric chloride, 5 to 7 parts of zinc chloride, 1 to 2 parts of yeast extracts, 0.3 to 0.6 part of phellodendron extracts and 1 to 2 parts of catechin. The microbial bacterium has the advantages that the matching is reasonable; the livestock and poultry manure can be effectively treated and decomposed.

Description

Microbial bacteria and its application
Technical field
The invention belongs to microbial bacterial agent field.It is more particularly related to a kind of microbial bacteria and its application.
Background technology
With the needs of society, large-scale cultivation is into one of new industry.But one weighs very much in large-scale cultivation The problem wanted is the harmless treatment of feces of livestock and poultry.Feces of livestock and poultry is the main cause of aquaculture environmental pollution, therefore, with Nuisanceless, green and organic agriculture development, feces of livestock and poultry is treated as problem in the urgent need to address.
Bio-feritlizer, is, by microbial life activity, to make crops obtain the product of specific fertilizer effect, is also claimed For Inoculant or bacterial manure, itself does not contain nutrient, it is impossible to replace fertilizer, it is both containing the nutrition unit needed for crop Element, and the product containing microorganism, be biological, organic and inorganic combination it can replace fertilizer, there is provided crop growth send out Educate required all kinds of nutrients.The extensive application of fertilizer and agricultural chemicals pros and cons and is deposited for human, to promote what is beneficial and abolish what is harmful, science Family proposes " ecological agriculture ", and few use is done step-by-step in farmland or fertilizer and chemical insecticide is not used, and uses organic Bio-feritlizer and using microbial process prevention and elimination of disease and pests.The all of plants such as existing tame crops, vegetables are giving birth to Fertilizer is all be unable to do without in long process, and the existing fertilizer major part for using all is fertilizer.It is well known that fertilizer can be made to environment Into pollution, and Long-Time Service fertilizer can also cause hardened to soil.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantage that at least will be described later.
It is a still further object of the present invention to provide a kind of microbial bacterial agent, compatibility rationally, can effective process feces of livestock and poultry Decompose, the crude fibre, protein and other nutrition in short period decomposition excrement.
It is a still further object of the present invention to provide a kind of microbial bacterial agent, can solve the problem that the problem of soil compaction, and drop The content of low heavy metal in soil.
It is in order to realize these purposes of the invention and further advantage, there is provided a kind of microbial bacterial agent including following The raw material of weight portion:
Mixed biologic bacterium:Smelly aspergillus 0.01-0.02 part, aspergillus awamori 0.02-0.04 parts, colloid bacillus cereus 0.01- 0.02 part, rose yellow streptomycete 0.01-0.02 parts, bacillus benzoevorans 0.01-0.02 parts, bacillus megaterium 0.03-0.05 Part and more glutinous series bacillus 0.03-0.05 parts;
Other raw materials:Sweet cherry roots 60-70 parts, mangosteen peel powder 10-12 parts, copper sulphate 3-5 parts, iron chloride 2-4 parts, chlorination Zinc 5-7 parts, yeast extract 1-2 parts, phellodendron extract 0.3-0.6 parts and catechin 1-2 part.
Preferably, its preparation method is comprised the following steps:
1) sweet cherry roots 60-70 parts and mangosteen peel powder 10-12 parts are crossed into 150 mesh sieves, in a kettle. 6Mpa, protect at 80 DEG C 20min is held, is taken out;
2) by copper sulphate 3-5 parts, iron chloride 2-4 parts, zinc chloride 5-7 parts, yeast extract 1-2 parts, phellodendron extract 0.3-0.6 parts and catechin 1-2 part and step 1) in raw material mix, sterilizing;
3) by mixed biologic bacterium and step 2) in raw material mix, it is 65% control water content, little in 40 DEG C of bottom fermentation 24-30 When, period is passed through 45 DEG C of heated sterile air every 2h, and stirs fermentate, and the intake of heated sterile air is per kilogram of fermentation 1.25m is passed through in thing3Heated sterile air, after the completion of fermentation, it is 15% to dry at 35 DEG C to water content, is obtained final product;
Wherein, the mixed biologic bacterium bag is included:Smelly aspergillus 0.01-0.02 part, aspergillus awamori 0.02-0.04 parts, colloid bud Spore bacillus 0.01-0.02 parts, rose yellow streptomycete 0.01-0.02 parts, bacillus benzoevorans 0.01-0.02 parts, huge gemma bar Bacterium 0.03-0.05 parts and more glutinous series bacillus 0.03-0.05 parts.
The object of the invention is also resided in and provides a kind of method for making fertilizer using described microbial bacterial agent, will be described micro- Bacteria agent and feces of livestock and poultry, rice bran in mass ratio 3:500:100 proportionings, after mixing turning fermentation bed is placed in, and is issued at 35 DEG C Ferment 3 days, obtains the first fermentate;Filter unnecessary zymotic fluid, make the first fermentate water content be 70%, backward first fermentate Middle addition filter mud, Adding Way is:One layer of first fermentate is spread in fermentation vat bottom, a metafiltration mud is re-layed, is staggeredly spread successively If the first fermentation nitride layer is 5 with the thickness ratio of filter mud layer:2, laying continues to ferment 25 days after finishing, and secondary fermentation temperature is 45 DEG C, it is 30% by fermentate drying to water content after the completion of fermentation.
Preferably, the filter mud is pre-processed, and the water content for controlling the filter mud is 70%, and is carried with pomelo peel Take thing, photocatalyst in mass ratio 10:1:1 mixing.
The present invention also aims to using described microbial bacterial agent rehabilitating soil content of beary metal application.
The present invention also aims to using described microbial bacterial agent in the hardened application of rehabilitating soil.
The present invention at least includes following beneficial effect:
1) using the present invention microbial bacterial agent can effective rehabilitating soil hardened problem, reduce the soil weight, increase native Earth aeration porosity.
2) there can be the content of beary metal for improving soil using the microbial bacterial agent of the present invention, especially to metal Cd, Cr, Pb energy Effectively degraded, while apply microbial bacterial agent using the method for the present invention, its degraded heavy metal it is better substantially.
3) sweet cherry roots and mangosteen peel part are carried out in a kettle. in the preparation process of microbial bacterial agent of the invention Pretreatment, has certain impact on its physical arrangement, accelerates its reproduction speed in the sweat of later stage bacteria agent, Speed improves 3%.
The further advantage of the present invention, target and feature embody part by description below, and part will also be by this The research of invention and practice and be understood by the person skilled in the art.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification Word can be implemented according to this.
It should be noted that experimental technique described in following embodiments, if no special instructions, is conventional method, institute Reagent and material are stated, if no special instructions, is commercially obtained.
Bacterial classification used of the invention can be obtained in market, wherein, smelly aspergillus, aspergillus awamori, bacillus benzoevorans, more glutinous class Bacillus (ATCC8524), bacillus megaterium (ATCC14581) are provided by Shanghai Fu Xiang bio tech ltd, colloid Bacillus is provided by auspicious paddy biotechnology, and rose yellow streptomycete is provided by Beijing North Na Chuanlian Bioteknologisk Institut.
Embodiment 1
A kind of microbial bacterial agent, is made up of the raw material of following weight portion:Mixed biologic bacterium:0.01 part of smelly aspergillus, bubble contain bent Mould 0.02 part, 0.01 part of colloid bacillus cereus, 0.01 part of rose yellow streptomycete, 0.01 part of bacillus benzoevorans, huge gemma bar 0.03 part of bacterium and more glutinous 0.03 part of series bacillus.Other raw materials:60 parts of sweet cherry roots, 10 parts of mangosteen peel powder, 3 parts of copper sulphate, 1 part of 2 parts of iron chloride, 5 parts of zinc chloride, 1 part of yeast extract, 0.3 part of phellodendron extract and catechin.Preparation method is:Will Mixed biologic bacterium and other raw materials are obtained final product by the mixing of above-mentioned weight portion.
Embodiment 2
A kind of microbial bacterial agent, preparation method is comprised the following steps:
1) by 70 parts of sweet cherry roots and 12 parts of mesh sieves of mistake 150 of mangosteen peel powder, in a kettle. 6Mpa, keep at 80 DEG C 20min, takes out.
2) by 5 parts of copper sulphate, 4 parts of iron chloride, 7 parts of zinc chloride, 2 parts of yeast extract, 0.6 part of phellodendron extract and youngster 2 parts of theine and step 1) in raw material mix, sterilizing.
3) by mixed biologic bacterium and step 2) in raw material mix, it is 65% control water content, in 40 DEG C of bottom fermentations 26 hours, Period is passed through 45 DEG C of heated sterile air every 2h, and stirs fermentate, and the intake of heated sterile air is every kilogram of fermentate In be passed through 1.25m3Heated sterile air, after the completion of fermentation, it is 15% to dry at 35 DEG C to water content, is obtained final product.
Wherein, the mixed biologic bacterium bag is included:0.02 part of smelly aspergillus, 0.04 part of aspergillus awamori, colloid bacillus cereus 0.02 Part, 0.02 part of rose yellow streptomycete, 0.02 part of bacillus benzoevorans, 0.05 part of bacillus megaterium and more glutinous series bacillus 0.05 part.
Embodiment 3
The method that a kind of microbial bacterial agent of utilization embodiment 2 makes fertilizer, by the microbial bacterial agent and animal dung Just, rice bran in mass ratio 3:500:100 proportionings, after mixing turning fermentation bed is placed in, and in 35 DEG C of bottom fermentations 3 days, obtains the first fermentation Thing;Filter unnecessary zymotic fluid, make the first fermentate water content be 70%, backward first fermentate in add filter mud, add Method is:One layer of first fermentate is spread in fermentation vat bottom, a metafiltration mud is re-layed, is staggeredly laid successively, the first fermentation nitride layer It is 5 with the thickness ratio of filter mud layer:2, laying continues to ferment 25 days after finishing, and secondary fermentation temperature is 45 DEG C, after the completion of fermentation, will Fermentate drying to water content is 30%.
Embodiment 4
The method that a kind of microbial bacterial agent described in utilization embodiment 1 makes fertilizer, by the microbial bacterial agent and poultry Poultry manure, rice bran in mass ratio 3:500:100 proportionings, after mixing turning fermentation bed is placed in, and in 35 DEG C of bottom fermentations 3 days, obtains first Fermentate;Filter unnecessary zymotic fluid, make the first fermentate water content be 70%, backward first fermentate in add filter mud, Adding Way is:One layer of first fermentate is spread in fermentation vat bottom, a metafiltration mud is re-layed, is staggeredly laid successively, the first fermentation Nitride layer is 5 with the thickness ratio of filter mud layer:2, laying continues to ferment 25 days after finishing, and secondary fermentation temperature is 45 DEG C, and fermentation is completed Afterwards, it is 30% by fermentate drying to water content.The filter mud is pre-processed, and the water content for controlling the filter mud is 70%, and with shaddock bark extract, photocatalyst in mass ratio 10:1:1 mixing.
The fertilizer that embodiment 4 is produced is stacked in colony house, is highly 60 centimeters, and temperature maintains 38 DEG C, places 10 Hour, the air conditions of colony house are detected afterwards, as a result show, in colony house, hydrogen sulfide average content is 0.13mg/m3, ammonia put down Content is 0.08mg/m3, the stink of fertilizer can't smell substantially.
Test 1 hardened soil water experiment
The field of three pieces of soil compactions is selected, the soil weight in selected field is 1.8 or so, and porosity is 24% or so, Aeration porosity is 8% or so, and in October, 2014 to 5 monthly kinds of wheats in 2015, in June, 2015 was to the 10 monthly spare time in 2015 Put, holding soil moisture of periodically watering is 62%.One of field is before wheat planting and in June, 2015 applies respectively a deuterzooid The microbial bacterial agent of embodiment 1, another piece of field is before wheat planting and in June, 2015 applies respectively the micro- of the present embodiment 2 Bacteria agent, usage amount is per square metre and applies 6kg microbial bacterial agents, is left one piece of field and do not apply any microbial bacteria Agent, calculates effect of increasing production and soil parameters after harvesting wheat.As a result it is to apply the field of the bacteria agent of embodiment 1 compared to not having The field of administration, wheat increase yield 7.1%, the field soil weight for applying bacteria agent is 1.2, and total porosity is 52%, ventilation Porosity is 18%.The field of the bacteria agent of embodiment 2 is applied compared to without the field applied, wheat increase yield 8.3% is applied The field soil weight of bacteria agent is 1.1, and total porosity is 55%, and aeration porosity is 20%.
Test 2 Nanning heavy-metal contaminated soil reparative experiments
Comparative example 1, in March, 2014 by unpolluted soil and No. 1 heavy metal pollution soil according to 1:30 quality Than mixing, and rotary tillage, pseudo-ginseng seedling is sowed after one month.
Embodiment 5, in March, 2014 by the bacteria agent of embodiment 2 and No. 2 heavy metal polluted soil surface layer 30cm soil According to 1:30 mass ratio mixing, and rotary tillage, sow pseudo-ginseng seedling after one month.
In October, 2014 checks pseudo-ginseng seedling growing state, wherein, the survival rate of pseudo-ginseng is 47.3% in comparative example 1, is implemented The survival rate of pseudo-ginseng is 70.1% in example 5.October 23 took at random comparative example 1 and the pseudo-ginseng seedling of embodiment 5 is each 8 plants, determined three The content of beary metal of soil, the results are shown in Table 1 in content of beary metal in seven seedlings, and correspondence example.
Content of beary metal in the soil of table 1 and pseudo-ginseng seedling
Test impact of 3 fertilizers to rice yield
The field experiment done in Nanning by 2014, the fertilizer made with the biological bacteria bought on the market is as control Group 2, compares with the fertilizer of the production of embodiment 3,4, and other field management methods are identical, and rice yield is shown in Table 2.
Table 2
Experimental group Ten thousand/mu of effective fringe Total grain panicle number Setting percentage % Actual production kg/ mu
Comparative example 2 20.33 93.12 83.56 560.47
Embodiment 3 28.97 97.89 89.42 678.41
Embodiment 4 29.33 97.66 88.76 668.59
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in specification and embodiment With, it can be applied to completely various suitable the field of the invention, for those skilled in the art, can be easily Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited In specific details and shown here as the embodiment with description.

Claims (6)

1. a kind of microbial bacterial agent, it is characterised in that including the raw material of following weight portion:
Mixed biologic bacterium:Smelly aspergillus 0.01-0.02 part, aspergillus awamori 0.02-0.04 parts, colloid bacillus cereus 0.01-0.02 parts, Rose yellow streptomycete 0.01-0.02 parts, bacillus benzoevorans 0.01-0.02 parts, bacillus megaterium 0.03-0.05 parts and stick more Series bacillus 0.03-0.05 parts;
Other raw materials:Sweet cherry roots 60-70 parts, mangosteen peel powder 10-12 parts, copper sulphate 3-5 parts, iron chloride 2-4 parts, zinc chloride 5- 7 parts, yeast extract 1-2 parts, phellodendron extract 0.3-0.6 parts and catechin 1-2 part.
2. microbial bacterial agent as claimed in claim 1, it is characterised in that its preparation method is comprised the following steps:
1) sweet cherry roots 60-70 parts and mangosteen peel powder 10-12 parts are crossed into 150 mesh sieves, in a kettle. 6Mpa, keep at 80 DEG C 20min, takes out;
2) by copper sulphate 3-5 parts, iron chloride 2-4 parts, zinc chloride 5-7 parts, yeast extract 1-2 parts, phellodendron extract 0.3-0.6 Part and catechin 1-2 part and step 1) in raw material mix, sterilizing;
3) by mixed biologic bacterium and step 2) in raw material mix, it is 65% control water content, in 40 DEG C of bottom fermentation 24-30 hours, Period is passed through 45 DEG C of heated sterile air every 2h, and stirs fermentate, and the intake of heated sterile air is every kilogram of fermentate In be passed through 1.25m3Heated sterile air, after the completion of fermentation, it is 15% to dry at 35 DEG C to water content, is obtained final product;
Wherein, the mixed biologic bacterium bag is included:Smelly aspergillus 0.01-0.02 part, aspergillus awamori 0.02-0.04 parts, colloid gemma bar Bacterium 0.01-0.02 parts, rose yellow streptomycete 0.01-0.02 parts, bacillus benzoevorans 0.01-0.02 parts, bacillus megaterium 0.03-0.05 parts and more glutinous series bacillus 0.03-0.05 parts.
3. the method that a kind of arbitrary described microbial bacterial agent of utilization claim 1-2 makes fertilizer, it is characterised in that by institute State microbial bacterial agent and feces of livestock and poultry, rice bran in mass ratio 3:500:100 proportionings, are placed in fermentation bed, at 35 DEG C after mixing turning Bottom fermentation 3 days, obtains the first fermentate;Filter unnecessary zymotic fluid, make the first fermentate water content be 70%, backward first The filter mud, Adding Way is added to be in ferment thing:One layer of first fermentate is spread in fermentation vat bottom, a metafiltration mud is re-layed, is handed over successively Mistake laying, the first fermentation nitride layer is 5 with the thickness ratio of filter mud layer:2, laying continues to ferment 25 days after finishing, secondary fermentation temperature It is 30% by fermentate drying to water content after the completion of fermentation for 45 DEG C.
4. the method that microbial bacterial agent as claimed in claim 3 makes fertilizer, it is characterised in that the filter mud has carried out pre- Process, control the filter mud water content be 70%, and with shaddock bark extract, photocatalyst in mass ratio 10:1:1 mixing be Can.
5. application of the arbitrary described microbial bacterial agent of claim 1-2 in rehabilitating soil content of beary metal.
6. the arbitrary described microbial bacterial agent of claim 1-2 is in the hardened application of rehabilitating soil.
CN201611118123.0A 2016-12-07 2016-12-07 Microbial bacterium and application thereof Pending CN106631577A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111154661A (en) * 2020-01-16 2020-05-15 中化农业(临沂)研发中心有限公司 Complex microbial inoculant and application thereof
CN113510144A (en) * 2021-04-22 2021-10-19 贵州星硕铭越环保科技有限公司 Method for treating heavy metal pollution of agricultural land in zinc smelting pollution area by soil method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101629156A (en) * 2009-08-26 2010-01-20 宋彦耕 Microbial agent and soil modifying agent produced by fermentation thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101629156A (en) * 2009-08-26 2010-01-20 宋彦耕 Microbial agent and soil modifying agent produced by fermentation thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111154661A (en) * 2020-01-16 2020-05-15 中化农业(临沂)研发中心有限公司 Complex microbial inoculant and application thereof
CN111154661B (en) * 2020-01-16 2021-09-21 中化农业(临沂)研发中心有限公司 Complex microbial inoculant and application thereof
CN113510144A (en) * 2021-04-22 2021-10-19 贵州星硕铭越环保科技有限公司 Method for treating heavy metal pollution of agricultural land in zinc smelting pollution area by soil method

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