CN1066165A - A kind of breeding method of hybrid rice - Google Patents

A kind of breeding method of hybrid rice Download PDF

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Publication number
CN1066165A
CN1066165A CN 92103511 CN92103511A CN1066165A CN 1066165 A CN1066165 A CN 1066165A CN 92103511 CN92103511 CN 92103511 CN 92103511 A CN92103511 A CN 92103511A CN 1066165 A CN1066165 A CN 1066165A
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high resistance
source
hybridize
excellent anti
hybrid rice
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CN 92103511
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CN1028472C (en
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周毓珍
陆作楣
丁伦友
赵霭林
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Nanjing Agricultural University
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Nanjing Agricultural University
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Abstract

The present invention relates to the selection of high resistance to white leaf-blast of hybrid rice " Shan excellent anti-65 ".Utilize DV 85Derive anti-source and bright extensive 63 hybridization are bred and are recovered system " Nan Nong anti-65 ", and hybridize with Zhenshan 97a " Nan Nong anti-65 ", and crossbreed is used for producing in preparation " Shan excellent anti-65 ".Show through evaluation, " Nan Nong anti-65 " coordinate force height, disease-resistant performance is at F 1Effective expression.It is close with " Shanyou 63 " with the fertility characteristic that " Shan excellent anti-65 " plants dwarf's form, at lesion performance high resistance to hoja blanca, increases production 20-50%.The present invention is suitable for hybrid rice district, China south and applies, and saves agricultural cost, man-hour, reduces pesticide pollution.

Description

A kind of breeding method of hybrid rice
The present invention relates to the selection of the new combination of hybrid rice of high resistance to hoja blanca.
More than 100,000,000 mu of Yangtze river basin medium rice area, hybridization long-grained nonglutinous rice proportion is bigger, and applied combination is single.Account for more than 90% as Jiangsu, Anhui two provinces " Shanyou 63 "." Shanyou 63 " also has as late rice or early rice in southern most of area, is the combination that present China hybrid rice is used the area maximum.This makes up high yield, adaptability is strong, has made outstanding contributions over past ten years on Rice Production.But because the white rot of its easy infection, output is high and unstable, especially retransmits the district at province bacterial leaf-blight Chang Faqu such as Soviet Union, Anhui, Hubei Province, Henan, has been difficult to use on producing.Along with agricultural production develops to efficient, low consumption direction, the new combination of the hybrid rice of seed selection high resistance to hoja blanca has become key subjects in the breeding.Domestic and international many breeding work persons have made big quantity research for this reason, seed selection in recent years the recovery system of many bacterial blight-resistings as IR system (comprising bright extensive 63), special green grass or young crops etc.Yet how because of still not disease-resistant or middle bacterial blight-resistings such as the hybrid combination Shanyou 63 that utilizes this incomplete dominant gene of Xa-4 to prepare, Shanyougui 33 (see " rice in China kind and pedigree thereof " that Shanghai science tech publishing house publishes in March, 1991 P.197-209).
The objective of the invention is to solve in the present production the not a great problem of bacterial blight-resisting of hybrid rice improved seeds " Shanyou 63 ", a kind of breeding method of high resistance to hoja blanca is provided, breeding high resistance to white leaf-blast of hybrid rice newly makes up, make it both to keep the merit of " Shanyou 63 ", again can high resistance to hoja blanca, go up large-area applications for producing.
The breeding method of high resistance to white leaf-blast of hybrid rice new varieties comprises and selects disease-resistant material, the transformation of anti-source is fastened to the good recovery of comprehensive proterties, then in the preparing hybrid kind.
With coming (TN No. one in the platform 1) be maternal, the DV of bacterial blight-resisting 85Hybridize anti-source " TN in the middle of breeding for male parent 1/ DV 85".
With the anti-source " TN in centre 1/ DV 85" be maternal, bright extensive 63 hybridize for male parent, breed high resistance to hoja blanca and recover system's " Nan Nong resists 65 ".
With male sterile line Zhenshan 97a or imperial Mortopl A or Xieqingzao A is maternal, recovers system " Nan Nong anti-65 " and hybridizes for male parent, preparing hybrid kind " Shan excellent anti-65 " or " special excellent anti-65 " or " assist excellent resist 65 ".
Middle anti-source " TN 1/ DV 85" hybridize with bright extensive 63 after, and carry out backcrossing for 3-4 time, breed high resistance to hoja blanca and recover system's " Nan Nong resists 65 ".
Be that example is narrated advantage of the present invention and good effect with " Shan excellent anti-65 " below:
1, high resistance to hoja blanca.The hybrid rice that the present invention breeds newly makes up " Shan excellent anti-65 " and not only has " Shanyou 63 " good integral economic trait, and the performance high resistance to hoja blanca.In 1 day sick level of strong fungus strain " Zhejiang 73 " inoculation offspring is 0.7 grade, is 3.9 grades and contrast " Shanyou 63 " sick level.
2, high yield.Because " Shan excellent anti-65 " newly makes up bacterial blight-resisting, the surface adapts to wide with regard to property, and yielding ability is good.At bacterial leaf-blight Chang Faqu, " Shan excellent anti-65 " because high resistance to hoja blanca, anaphase blade is delicate and pretty, and production ratio " Shanyou 63 " (CK) improves 20-50%." Shan excellent anti-65 " because high resistance to hoja blanca has overcome " Shanyou 63 " unique defective on producing, its integral economic trait also is better than other hybrid rice combination.
Accompanying drawing is: high resistance to hoja blanca hybrid rice breeding line spectrogram.
Be described in detail the embodiment of the invention below:
The used bacterial blight-resisting material of the present invention DV 85For introducing the anti-source long-grained nonglutinous rice (seeing " rice in China kind and pedigree thereof " .P.276) in the rice varieties.This kind contains Xa- 5.Xa- 7The disease-resistant gene of two different in kinds, performance high resistance to hoja blanca, and disease-resistant stable, high stalk, drape over one's shoulders leaf, integral economic trait is poor, directly utilizes relatively more difficult in breeding for disease resistance.With DV 85With coming (TN No. one in the susceptible variety platform 1) hybridization, transformation becomes semi-short-stalked, the anti-source TN in the centre of high resistance to hoja blanca 1/ DV 85With the anti-source TN in centre 1/ DV 85When being booting, affiliated strain carries out the leaf-cutting inoculation with bacterial strain 300,000,000/ml concentration such as bacterial leaf-blight KS-6-6, Zhejiang 173, the leaf-cutting position is for falling a leaf, minority is for falling two leaves, every strain inoculation 1-3 leaf, inoculating the number of blade in 2 years respectively is 618,129, inoculate back 20 days and pluck the inoculation blade, scab linear measure longimetry and the analysis expanded, the scab average length is respectively 4.65 ± 0.192cm, 3.03 ± 0.206cm.Middle anti-source TN 1/ DV 85Not only showing it has stronger resistance, and has good transformation effect as anti-source donor.
With DV 85Anti-source " TN derives 1/ DV 85" be maternal, hybridize with male parent bright extensive 63, carry out again backcrossing for 3-4 time, breeding recovery is kind " Nan Nong anti-65 "." Nan Nong anti-65 " not only has bright extensive 63 as recovering the integral economic trait advantage that system is had, and resistance, and disease-resistant performance is expressed under male sterile line genetic background effectively, and coordinate force is good, and pollen amount is big, and the florescence is long, the favourable production of hybrid seeds.
Table one:
Kind Plant height cm Single-strain tassel number Spike length (cm) Total grain Ripening rate % Thousand kernel weight Bacterial leaf-blight gradually 173
Nan Nong anti-65 103.6 13.43 22.3 137.3 78.77 27.9 0.6
Bright extensive 63 101.3 14.73 21.4 101.9 78.57 28.6 2.1
With male sterile line Zhenshan 97a or imperial Mortopl A or Xieqingzao A is female parent, and recovers system " Nan Nong anti-65 " male parent and hybridizes combo, thereby breed novel combination high resistance to white leaf-blast of hybrid rice kind." Shan excellent anti-65 " plant forms and fertility characteristic are close with " Shanyou 63 ", and be 130-140 days the time of infertility, about plant height 110cm, plant type is concentrated, and blade is bigger, and sword-like leave is soft slightly, tillering ability is strong, property in great numbers is good, and anaphase blade is delicate and pretty, and the big grain of fringe is big, thousand kernel weight 28-31g, rice matter is more excellent, wide adaptability, and yielding ability is good.
Implementing best way of the present invention is with anti-source material DV 85Be male parent, to come (TN No. one in the platform 1) hybridize filial generation " TN for female parent 1/ DV 85" be middle anti-source; Again with TN 1/ DV 85Be female parent, with bright extensive 63 paternal hybrids, backcross and breed for 3-4 time then " Nan Nong anti-65 ", be female parent with male sterile line Zhenshan 97a or imperial Mortopl A or Xieqingzao A at last, to recover system " Nan Nong anti-65 " is that male parent is carried out hybrid seeding, directly applies to large tracts of land production thereby obtain the high resistance to white leaf-blast of hybrid rice new varieties.
Be example with Shan excellent anti-65 below:
Jiangpu, Jiangsu Province county belongs to bacterial leaf-blight Chang Faqu, plants experimentally " Shan excellent anti-65 " in 1991, and test identifies and show that " Shan excellent anti-65 " compares the significantly volume increase (table two, table three) according to (CK):
Table two:
Kind Plant height cm Effective fringe ten thousand/mu Every fringe grain husk is spent number The solid number of every fringe
Shan excellent anti-65 112.3 16.38 157.5 148.7
Shanyou 63 (CK1) 111.4 16.92 145.4 119.9
Shanyougui 33 (CK2) 103.0 16.74 142.7 138.2
Table three:
Kind Ripening rate % Thousand kernel weight g Bacterial leaf-blight level Zhejiang 173 Output Kg/ mu
Shan excellent anti-65 93.9 28.6 0.7 697.5
Shanyou 63 (CK1) 83.1 28.1 3.9 576.1
Shanyougui 33 (CK2) 89.0 27.3 2.7 631.6
The Jianhu County, Jiangsu Province also belongs to bacterial leaf-blight Chang Faqu, and " Shan excellent anti-65 " planted experimentally in production in 1991, and the result shows that (CK) same significantly volume increase (table four, table five) is shone in " Shan excellent anti-65 " performance comparison:
Table four:
Kind Plant height cm Effective fringe ten thousand/mu Every grain husk is spent number The solid number of every fringe
Shan excellent anti-65 111.5 16.89 158.4 149.5
Shanyou 63 (CK 1) 110.0 16.74 144.4 118.7
Shanyougui 33 (CK 2) 102.0 16.74 141.7 137.2
Table five:
Kind Ripening rate % Thousand kernel weight (g) Bacterial leaf-blight level Zhejiang 173 Output Kg/ mu
Shan excellent anti-65 95.1 29.2 0.7 705.6
Shanyou 63 (CK 1) 84.2 27.1 3.9 580.1
Shanyougui 33 (CK 2) 89.0 27.3 2.7 632.7
Excellent anti-65 seeding techniques of Shan are identical with Shanyou 63 with culture technique.

Claims (2)

1, a kind of breeding method of high resistance to white leaf-blast of hybrid rice comprises and selects disease-resistant material, and the transformation of anti-source is fastened to the good recovery of comprehensive proterties, and preparing hybrid kind then is characterized in that:
(a), with coming (TN No. one in the platform 1) be maternal, the DV of bacterial blight-resisting 85Be male parent, hybridize, anti-source " TN in the middle of breeding 1/ D 85";
(b), with middle anti-source " TN 1/ DV 85" be maternal, bright extensive 63 hybridize for male parent, breed high resistance to hoja blanca and recover system's " Nan Nong resists 65 ";
(c), be maternal with male sterile line Zhenshan 97a or imperial Mortopl A or Xieqingzao A, recover system " Nan Nong anti-65 " and hybridize, preparing hybrid kind " Shan excellent anti-65 " or " special excellent anti-65 " or " assist excellent resist 65 " for male parent.
2, breeding method according to claim 1, anti-source " TN in the middle of it is characterized in that 1/ DV 85" hybridize with bright extensive 63 after, carry out again backcrossing for 3-4 time, breed high resistance to hoja blanca and recover system's " Nan Nong resists 65 ".
CN 92103511 1992-05-16 1992-05-16 Breeding method for hybrid rice Expired - Fee Related CN1028472C (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1039769C (en) * 1993-11-10 1998-09-16 湖南杂交小麦研究中心 Method for producing two-line hybrid rice seeds
CN1075345C (en) * 1996-09-18 2001-11-28 南京农业大学 Breeding method for high resistance to white leaf-blast of hybrid rice
CN101796918A (en) * 2009-03-12 2010-08-11 江苏丘陵地区镇江农业科学研究所 Breeding and seed production methods of quality multi-resistant high-yield hybrid rice
CN116602205A (en) * 2023-05-25 2023-08-18 福建省农业科学院水稻研究所 Breeding method of new disease-resistant rice material

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100589695C (en) * 2007-06-15 2010-02-17 广东省农业科学院作物研究所 Slective seeding method for super-high yield peanut

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1039769C (en) * 1993-11-10 1998-09-16 湖南杂交小麦研究中心 Method for producing two-line hybrid rice seeds
CN1075345C (en) * 1996-09-18 2001-11-28 南京农业大学 Breeding method for high resistance to white leaf-blast of hybrid rice
CN101796918A (en) * 2009-03-12 2010-08-11 江苏丘陵地区镇江农业科学研究所 Breeding and seed production methods of quality multi-resistant high-yield hybrid rice
CN116602205A (en) * 2023-05-25 2023-08-18 福建省农业科学院水稻研究所 Breeding method of new disease-resistant rice material

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