CN106615615A - Mixed microbial fermented feed and production method thereof - Google Patents
Mixed microbial fermented feed and production method thereof Download PDFInfo
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Abstract
The invention relates to the technical filed of microbial fermentation, and discloses a mixed microbial fermented feed and a method for producing the mixed microbial fermented feed by adopting aflatoxin in mixed microbial degraded moldy feeds. By adopting the aflatoxin in the mixed microbial degraded moldy feeds to prepare the mixed microbial fermented feed, the mildewed raw materials or products are recycled by using micro-ecological preparations so as to prevent discarding or wasting thereof; and the product qualities are also improved. The production method of the mixed microbial fermented feed disclosed by the invention ensures 100% degradation of the aflatoxin in the moldy feeds; moreover, the mixed flora added during the production processes can be used as probiotics even if the mixed flora are not completely consumed for degrading the aflatoxin, so that probiotics are provided for the intestines of the livestock and the poultry so as to promote growth of the animals and improve disease resistance of the livestock and the poultry. The nutritional values of the feed are greatly improved, and the nutritional characteristics and safety of the feed are ensured.
Description
Technical field
The present invention relates to technical field of microbial fermentation, it is a kind of mixed microorganism fermented feed and adopts mixed microorganism
Degraded is gone mouldy the method that aflatoxin produces mixed microorganism fermented feed in feed.
Background technology
Go mouldy and reduce the nutritive value of feed, have impact on palatability, more seriously cause feed product mould poison
It is plain exceeded, suppress the production performance of animal, reducing the immunity of animal, it might even be possible to which health is threatened by food chain.It is mould
To human hazard, livestock and poultry have eaten the feed for going mouldy to verticillium toxin major part, can have residual in meat, eggs and milk, by food chain to people
Body health works the mischief, and causes deformity, canceration, immunosupress etc..Aflatoxin is typical main mould in mycotoxin
Bacterium, it is the main metabolites in mammal body, is present in the edible parts such as milk, liver, the eggs of animal, is common in breast
In juice, it has very strong toxicity and carcinogenicity, because breast and dairy products are the staple foods of infant, and the function of detoxification device of infant
Official develops imperfection, and to the health of infant directly threat can be produced.Estimate that the whole world is every year about according to world food tissue
There are the agricultural product such as 5%～7% grain, feed to be encroached on by mould, cause hundreds billion of dollars of economic loss.
Processing the method for the feed that goes mouldy at present mainly includes physical treatment method, chemical treatment method and microbiological treatment side
Method.Physical treatment method mainly removes the aflatoxin gone mouldy in feed by the method for high temperature, but goes mouldy in removal
While aflatoxin in feed, high temperature also easily causes the denaturation of the feed protein that goes mouldy, so as to reduce process
The nutritive value of the feed that goes mouldy afterwards, meanwhile, also cause to put into substantial amounts of energy consumption in production process using physical treatment method, increase
Add production cost, it is difficult to control, be unsuitable for actual production.Chemical treatment method is mainly by additionization in the feed that goes mouldy
Learn inorganic agent, mould inhibitor, de- mould dose etc., remove aflatoxin for example with formaldehyde, but stablizing due to aflatoxin
Property it is strong, substantial amounts of chemical treatments, one side high cost, the chemical treatments on the other hand adding need to be added to remain in process
Going mouldy in feed afterwards, it is difficult to extract, if feed of the animal using the chemical treatments containing residual, will certainly be to animal
Growth is adversely affected.Microbiological treatment means are a kind of higher processing means of current cost performance, and it adopts microorganism to drop
Solution is gone mouldy the aflatoxin in feed, on the premise of feed protein nutrient content is ensured, both will not put into it is too high into
This, do not result in the residual of chemical treatments yet, at present frequently with microbiological treatment means, for example with lactic acid bacteria adhesion,
Its aflatoxin degradation rate is up to 70%, and it simply adheres to toxin, it is impossible to control fungus growth；Using Candida
Bacterium processes the material that goes mouldy, and its aflatoxin degradation rate is up to 80%；The material that goes mouldy is processed using red bar bacterium, its aspergillus flavus poison
Plain degradation rate is up to 99%, but it need to be fermented in a liquid, it is difficult to industrial implementation, is unsuitable for actual production；Using true
Bacterium E-2 process is gone mouldy material, and its aflatoxin degradation rate is up to 99%, but its working condition is harsh, it is difficult to control, uncomfortable
In actual production.Therefore, current microbiological treatment means frequently be single bacterial classification processing means, single culture place
Time-consuming, aflatoxin degradation efficiency is low, working condition is harsh, being unsuitable for actual production need to manage presence fermentation when going mouldy feed
The problem wanted.
The content of the invention
The invention provides a kind of mixed microorganism fermented feed, overcomes the deficiency of above-mentioned prior art, according to this
Bright residual of the mixed microorganism fermented feed without chemical reagent for obtaining, nutrient content are good, present invention also offers a kind of adopt
Mixed microorganism degraded is gone mouldy the method that aflatoxin produces mixed microorganism fermented feed in feed, is overcome above-mentioned existing
The deficiency of technology, its current single culture of energy effectively solving has fermentation when feed is gone mouldy in process, and time-consuming, aflatoxin
Degradation efficiency is low, working condition is harsh, be unsuitable for the problem of needs of production.
One of technical scheme is realized by following measures：A kind of mixed microorganism fermented feed, presses
Following methods are obtained：The first step, chooses two kinds in lactic acid bacteria, distiller's yeast saccharomycete, bacillus, mycobacterium and short shape bacillus
More than, the bacterium colony of the bacterial classification chosen is respectively put in the nutrient solution that spreads cultivation and is spread cultivation respectively；
Second step, after the single bacterium colony after two or more spreading cultivation is mixed mixed bacterial is obtained, in mixed bacterial plus
Enter bacterial classification growth accelerator and obtain leavening, the mass ratio between mixed bacterial and bacterial classification growth accelerator is 1:10 to 20；
3rd step, leavening is put into be stirred in temperature is 30 DEG C to 35 DEG C 10% to 15% brown sugar water is well mixed,
Then placement is entered line activating for 20 minutes and obtains activating bacterium solution, and the mass ratio between leavening and 10% to 15% brown sugar water is 1:
100 to 150；
4th step, the pending material that goes mouldy is crushed, and then adds activation bacterium solution in the material that goes mouldy after crushing, activates bacterium
The addition of liquid is to add 1 kilogram to 2 kilograms of activation bacterium solution in the material that goes mouldy crushed per 1000 kilograms, temperature be 25 DEG C extremely
It is uniformly mixed under the conditions of 45 DEG C and obtains treating fermentation material, then will treats that fermentation material loads feed fermentation bag；
5th step, be loaded into feed fermentation bag after fermentation material after temperature is 10 DEG C to 40 DEG C condition bottom fermentations 3 days to 30 days
Obtain mixed microorganism fermented feed.
The further optimization and/or improvements to one of foregoing invention technical scheme are presented herein below：
In the above-mentioned first step, 4 grams of sucrose, 4 grams of glucose, 4 grams of bean cake powders, 4g corn flour, 0.3g potassium dihydrogen phosphates are dissolved in
In 100 grams of physiological saline, the nutrient solution that spreads cultivation is obtained, the temperature for spreading cultivation is 34 DEG C to 36 DEG C.
In above-mentioned second step, the bacterium amount of mixed bacterial reaches 1 × 1015Individual/more than g.
In above-mentioned second step, bacterial classification growth accelerator is 30% to 40% FOS, 5% to 15% auxin and surplus
The mixture of ammonium hydrogen phosphate.
In above-mentioned 4th step, when after pending material process of going mouldy as chicken feed, pending feed powder of going mouldy is broken to
Granularity is less than less than 1.5 millimeters；When after pending material process of going mouldy as sheep feed or ox feed, by the pending material that goes mouldy
Granularity is crushed to for 2.0 millimeters to 2.5 millimeters；When after pending material process of going mouldy as pig feed, pending is gone mouldy
Feed powder is broken to granularity for 1 millimeter to 1.3 millimeters.
The two of technical scheme are realized by following measures：One kind is gone mouldy using mixed microorganism degraded
The method that aflatoxin produces mixed microorganism fermented feed in feed, is carried out in the steps below：The first step, chooses lactic acid
It is two or more in bacterium, distiller's yeast saccharomycete, bacillus, mycobacterium and short shape bacillus, by the bacterium colony of the bacterial classification chosen point
It is not put in the nutrient solution that spreads cultivation and is spread cultivation respectively；
Second step, after the single bacterium colony after two or more spreading cultivation is mixed mixed bacterial is obtained, in mixed bacterial plus
Enter bacterial classification growth accelerator and obtain leavening, the mass ratio between mixed bacterial and bacterial classification growth accelerator is 1:10 to 20；
3rd step, leavening is put into be stirred in temperature is 30 DEG C to 35 DEG C 10% to 15% brown sugar water is well mixed,
Then placement is entered line activating for 20 minutes and obtains activating bacterium solution, and the mass ratio between leavening and 10% to 15% brown sugar water is 1:
100 to 150；
4th step, the pending material that goes mouldy is crushed, and then adds activation bacterium solution in the material that goes mouldy after crushing, activates bacterium
The addition of liquid is to add 1 kilogram to 2 kilograms of activation bacterium solution in the material that goes mouldy crushed per 1000 kilograms, temperature be 25 DEG C extremely
It is uniformly mixed under the conditions of 45 DEG C and obtains treating fermentation material, then will treats that fermentation material loads feed fermentation bag；
5th step, be loaded into feed fermentation bag after fermentation material after temperature is 10 DEG C to 40 DEG C condition bottom fermentations 3 days to 30 days
Obtain mixed microorganism fermented feed.
To foregoing invention technical scheme two further optimization and/or improvements are presented herein below：
In the above-mentioned first step, 4 grams of sucrose, 4 grams of glucose, 4 grams of bean cake powders, 4g corn flour, 0.3g potassium dihydrogen phosphates are dissolved in
In 100 grams of physiological saline, the nutrient solution that spreads cultivation is obtained, the temperature for spreading cultivation is 34 DEG C to 36 DEG C.
In above-mentioned second step, the bacterium amount of mixed bacterial reaches 1 × 1015Individual/more than g.
In above-mentioned second step, bacterial classification growth accelerator is 30% to 40% FOS, 5% to 15% auxin and surplus
The mixture of ammonium hydrogen phosphate.
In above-mentioned 4th step, when after pending material process of going mouldy as chicken feed, pending feed powder of going mouldy is broken to
Granularity is less than less than 1.5 millimeters；When after pending material process of going mouldy as sheep feed or ox feed, by the pending material that goes mouldy
Granularity is crushed to for 2.0 millimeters to 2.5 millimeters；When after pending material process of going mouldy as pig feed, pending is gone mouldy
Feed powder is broken to granularity for 1 millimeter to 1.3 millimeters.
Aflatoxin obtains mixed microorganism fermentation feeding in the feed that gone mouldy by being degraded using mixed microorganism of the invention
Material, its one side probiotics can make mouldy raw material or product be utilized again and be not dropped or become waste product, and one
Aspect can also improve product quality, the present invention using mixed microorganism degraded go mouldy that aflatoxin production in feed obtains it is mixed
In closing fermentative feedstuff of microbe, the aflatoxin of the material that goes mouldy can 100% degraded, also, the mixing added in production process
Flora is while aflatoxin degradation, even if not consuming completely, also can provide intestinal beneficial for livestock and poultry as beneficial bacterium
Bacterium, promote growth of animal and improve livestock and poultry diseases prevention ability, greatly improve the nutritive value of feed, it is ensured that its nutritive peculiarity and
Security.
Specific embodiment
The present invention is not limited by following embodiments, can technology according to the present invention scheme and actual conditions determining specifically
Embodiment.Various chemical reagent and chemical article are previously mentioned in the present invention if no special instructions, are in prior art public
Know public chemical reagent and chemical article；Percentage in the present invention is as without specified otherwise, being mass percent；This
If the solution in bright is the aqueous solution that solvent is water without specified otherwise, for example, hydrochloric acid solution is aqueous hydrochloric acid solution.
With reference to embodiment, the invention will be further described：
Embodiment 1, the mixed microorganism fermented feed is gone mouldy the production of aflatoxin in feed using mixed microorganism degraded
Method is obtained, and is carried out in the steps below：The first step, chooses lactic acid bacteria, distiller's yeast saccharomycete, bacillus, mycobacterium and short shape
It is two or more in bacillus, the bacterium colony of the bacterial classification chosen is respectively put in the nutrient solution that spreads cultivation and is spread cultivation respectively；
Second step, after the single bacterium colony after two or more spreading cultivation is mixed mixed bacterial is obtained, in mixed bacterial plus
Enter bacterial classification growth accelerator and obtain leavening, the mass ratio between mixed bacterial and bacterial classification growth accelerator is 1:10 to 20；
3rd step, leavening is put into be stirred in temperature is 30 DEG C to 35 DEG C 10% to 15% brown sugar water is well mixed,
Then placement is entered line activating for 20 minutes and obtains activating bacterium solution, and the mass ratio between leavening and 10% to 15% brown sugar water is 1:
100 to 150；
4th step, the pending material that goes mouldy is crushed, and then adds activation bacterium solution in the material that goes mouldy after crushing, activates bacterium
The addition of liquid is to add 1 kilogram to 2 kilograms of activation bacterium solution in the material that goes mouldy crushed per 1000 kilograms, temperature be 25 DEG C extremely
It is uniformly mixed under the conditions of 45 DEG C and obtains treating fermentation material, then will treats that fermentation material loads feed fermentation bag；Feed fermentation bag can
With from presently commercially available feed fermentation bag or respiratory membrane fermentation bag；It is pending to go mouldy material for the agricultural crop straw that do not become and mould
Grain of change etc., can be the agricultural crop straw for going mouldy or the wheat bran for going mouldy or the corn for going mouldy or cotton dregs for going mouldy etc..
5th step, be loaded into feed fermentation bag treats that fermentation material is 10 DEG C to 40 DEG C condition bottom fermentations 3 days to 30 in temperature
Mixed microorganism fermented feed is obtained after it.Under the cooperation of suitable fermentation temperature and fermentation time, it is ensured that treat fermentation material energy
The aflatoxin in fermentation material is treated in enough fully fermentations, effectively degraded.
The present invention is gone mouldy the mixed microorganism fermentation that aflatoxin production is obtained in feed using mixed microorganism degraded
In feed, aflatoxin can be degraded to qualifying object value, and lower by 60% than national sector standard required standard value, and
And, the mixed bacterial added in production process is while aflatoxin degradation, even if not consuming completely, also can conduct
Beneficial bacterium, for livestock and poultry intestinal beneficial bacterium is provided, and is promoted growth of animal and is improved the diseases prevention ability of livestock and poultry, greatly improves feed
Nutritive value.
Embodiment 2, the mixed microorganism fermented feed is gone mouldy aflatoxin in feed using mixed microorganism degraded
Production method is obtained, and is carried out in the steps below：The first step, choose lactic acid bacteria, distiller's yeast saccharomycete, bacillus, mycobacterium and
It is two or more in short shape bacillus, the bacterium colony of the bacterial classification chosen is respectively put in the nutrient solution that spreads cultivation and is spread cultivation respectively；
Second step, after the single bacterium colony after two or more spreading cultivation is mixed mixed bacterial is obtained, in mixed bacterial plus
Enter bacterial classification growth accelerator and obtain leavening, the mass ratio between mixed bacterial and bacterial classification growth accelerator is 1:10 or 20；
3rd step, it is to be stirred to be well mixed in 30 DEG C or the 10% of 35 DEG C or 15% brown sugar water that leavening is put into into temperature,
Then placement is entered line activating for 20 minutes and obtains activating bacterium solution, and the mass ratio between leavening and 10% or 15% brown sugar water is 1:
100 or 150；
4th step, the pending material that goes mouldy is crushed, and then adds activation bacterium solution in the material that goes mouldy after crushing, activates bacterium
The addition of liquid is to add 1 kilogram or 2 kilograms of activation bacterium solution in the material that goes mouldy crushed per 1000 kilograms, temperature be 25 DEG C or
It is uniformly mixed under the conditions of 45 DEG C and obtains treating fermentation material, then will treats that fermentation material loads feed fermentation bag；
5th step, be loaded into feed fermentation bag after fermentation material after temperature is 10 DEG C or 40 DEG C of condition bottom fermentations 3 days or 30 days
Obtain mixed microorganism fermented feed.
Embodiment 3, as the optimization of above-described embodiment, in the first step, by 4 grams of sucrose, 4 grams of glucose, 4 grams of bean cake powders,
4g corn flour, 0.3g potassium dihydrogen phosphates are dissolved in 100 grams of physiological saline, obtain the nutrient solution that spreads cultivation, and the temperature for spreading cultivation is 34 DEG C
To 36 DEG C；Expanding can also prepare the nutrient solution with identical Spawn incubation effect with nutrient solution by this area professional technique；Select
The suitable temperature that spreads cultivation, can be the preferable growing environment of offer that spreads cultivation of bacterial classification.
Embodiment 4, used as the optimization of above-described embodiment, in second step, the bacterium amount of mixed bacterial reaches 1 × 1015Individual/g with
On；Mixed bacterial is obtained after single bacterium colony after two or more spreading cultivation is mixed, the bacterium amount for making mixed bacterial reaches 1 ×
1015Individual/more than g, can effectively meet and ensure the normal fermentation of feed of subsequently going mouldy, and the degraded for aflatoxin provides guarantor
Barrier.
Embodiment 5, used as the optimization of above-described embodiment, in second step, bacterial classification growth accelerator is 30% to 40% oligomeric fruit
The mixture of the ammonium hydrogen phosphate of sugar, 5% to 15% auxin and surplus；Auxin is that a class contains a unsaturated aromatic hydrocarbon
With the endogenous hormones of an acetic acid side chain, literary abbreviation IAA is international, is heteroauxin（IAA）.Chloro- IAA, 5- hydroxyls of 4--
IAA, methyl α-naphthyl acetate（NAA）, indolebutyric acid etc. be class auxin, play the role of to promote cell growth.
Embodiment 6, as the optimization of above-described embodiment, in the 4th step, as chicken feed after pending material process of going mouldy
When, pending feed powder of going mouldy is broken to into granularity less than less than 1.5 millimeters；The pending material that goes mouldy process after as sheep feed or
During ox feed, pending feed powder of going mouldy is broken to into granularity for 2.0 millimeters to 2.5 millimeters；The pending material that goes mouldy is made after processing
For pig feed when, pending feed powder of going mouldy is broken to into granularity for 1 millimeter to 1.3 millimeters.According to the forage feed of different animals
Require, pending feed powder of going mouldy is broken to the medicine feed feed granularity requirements for being suitable for different animals, ensureing to be provided for animal
On the premise of suitable nutrient fodder, the digestive system of animal is not interfered with.
Selecting a collection of 500 chick baby chicks to be divided into two groups carries out feeding experiment to having a competition, and one group of nursing is according to above-mentioned reality of the invention
Apply the mixed microorganism fermented feed that example is obtained, another group of nursing normal conventional feed, after one month, the survival rate of two groups of chickens is equal
More than 95%, two groups of body weight of growth is without significant difference；Illustrate that the mixed microorganism obtained according to the above embodiment of the present invention ferments
Feed nutrient is good, and product quality is stable.
The mixed microorganism fermented feed obtained according to the above embodiment of the present invention is sent to into respectively detection, as a result such as table 1
It is shown, wherein, the ox material that goes mouldy is the ox feed that goes mouldy, and the chicken material that goes mouldy is chicken feed of going mouldy.From data in table 1, aflatoxins
The clearance of poison reaches 100%, therefore, the aflatoxin degradation rate of the present invention is high, removal effect more preferably, and the present invention
The nutriments such as the original protein of feed will not be destroyed, feed nutritive value is high, safe and efficient pollution-free, meanwhile, according to this
The mixed microorganism fermented feed that inventive embodiments are obtained need not add any composition in feeding process, open and wrap instant, compared to
Conventinal breeding method, it is possible to decrease feeding cost 8% to 15%, feed spontaneous fermentation under anaerobic state in bag is that is, ecological and environmentally friendly.
Aflatoxin obtains mixed microorganism fermentation feeding in the feed that gone mouldy by being degraded using mixed microorganism of the invention
Material, its one side probiotics can make mouldy raw material or product be utilized again and be not dropped or become waste product, and one
Aspect can also improve product quality, the present invention using mixed microorganism degraded go mouldy that aflatoxin production in feed obtains it is mixed
In closing fermentative feedstuff of microbe, the aflatoxin of the material that goes mouldy can 100% degraded, also, the mixing added in production process
Flora is while aflatoxin degradation, even if not consuming completely, also can provide intestinal beneficial for livestock and poultry as beneficial bacterium
Bacterium, promote growth of animal and improve livestock and poultry diseases prevention ability, greatly improve the nutritive value of feed, it is ensured that its nutritive peculiarity and
Security.
Above technical characteristic constitutes embodiments of the invention, and it has stronger adaptability and implementation result, can basis
It is actually needed the non-essential technical characteristic of increase and decrease to meet the demand of different situations.
Claims (10)
1. a kind of mixed microorganism fermented feed, it is characterised in that obtain as follows：The first step, chooses lactic acid bacteria, distiller's yeast
It is two or more in saccharomycete, bacillus, mycobacterium and short shape bacillus, the bacterium colony of the bacterial classification chosen is respectively put into into expansion
Spread cultivation respectively in cultivation nutrient fluid；
Second step, after the single bacterium colony after two or more spreading cultivation is mixed mixed bacterial is obtained, in mixed bacterial plus
Enter bacterial classification growth accelerator and obtain leavening, the mass ratio between mixed bacterial and bacterial classification growth accelerator is 1:10 to 20；
3rd step, leavening is put into be stirred in temperature is 30 DEG C to 35 DEG C 10% to 15% brown sugar water is well mixed,
Then placement is entered line activating for 20 minutes and obtains activating bacterium solution, and the mass ratio between leavening and 10% to 15% brown sugar water is 1:
100 to 150；
4th step, the pending material that goes mouldy is crushed, and then adds activation bacterium solution in the material that goes mouldy after crushing, activates bacterium
The addition of liquid is to add 1 kilogram to 2 kilograms of activation bacterium solution in the material that goes mouldy crushed per 1000 kilograms, temperature be 25 DEG C extremely
It is uniformly mixed under the conditions of 45 DEG C and obtains treating fermentation material, then will treats that fermentation material loads feed fermentation bag；
5th step, be loaded into feed fermentation bag after fermentation material after temperature is 10 DEG C to 40 DEG C condition bottom fermentations 3 days to 30 days
Obtain mixed microorganism fermented feed.
2. mixed microorganism fermented feed according to claim 1, it is characterised in that in the first step, by 4 grams of sucrose, 4 grams
Glucose, 4 grams of bean cake powders, 4g corn flour, 0.3g potassium dihydrogen phosphates are dissolved in 100 grams of physiological saline, obtain the nutrient solution that spreads cultivation,
The temperature for spreading cultivation is 34 DEG C to 36 DEG C.
3. mixed microorganism fermented feed according to claim 1 and 2, it is characterised in that in second step, mixed bacterial
Bacterium amount reaches 1 × 1015Individual/more than g.
4. the mixed microorganism fermented feed according to claim 1 or 2 or 3, it is characterised in that in second step, bacterial classification growth-promoting
Long agent is the mixture of the ammonium hydrogen phosphate of 30% to 40% FOS, 5% to 15% auxin and surplus.
5. the mixed microorganism fermented feed according to claim 1 or 2 or 3 or 4, it is characterised in that in the 4th step, wait to locate
When after the material process of going mouldy of reason as chicken feed, pending feed powder of going mouldy is broken to into granularity less than less than 1.5 millimeters；It is pending
Go mouldy material process after as sheep feed or ox feed when, by pending feed powder of going mouldy be broken to granularity for 2.0 millimeters to 2.5 milli
Rice；When after pending material process of going mouldy as pig feed, pending feed powder of going mouldy is broken to into granularity for 1 millimeter to 1.3 millis
Rice.
6. a kind of employing mixed microorganism degraded is gone mouldy the method that aflatoxin in feed produces mixed microorganism fermented feed,
It is characterized in that carrying out in the steps below：The first step, chooses lactic acid bacteria, distiller's yeast saccharomycete, bacillus, mycobacterium and short shape
It is two or more in bacillus, the bacterium colony of the bacterial classification chosen is respectively put in the nutrient solution that spreads cultivation and is spread cultivation respectively；
Second step, after the single bacterium colony after two or more spreading cultivation is mixed mixed bacterial is obtained, in mixed bacterial plus
Enter bacterial classification growth accelerator and obtain leavening, the mass ratio between mixed bacterial and bacterial classification growth accelerator is 1:10 to 20；
3rd step, leavening is put into be stirred in temperature is 30 DEG C to 35 DEG C 10% to 15% brown sugar water is well mixed,
Then placement is entered line activating for 20 minutes and obtains activating bacterium solution, and the mass ratio between leavening and 10% to 15% brown sugar water is 1:
100 to 150；
4th step, the pending material that goes mouldy is crushed, and then adds activation bacterium solution in the material that goes mouldy after crushing, activates bacterium
The addition of liquid is to add 1 kilogram to 2 kilograms of activation bacterium solution in the material that goes mouldy crushed per 1000 kilograms, temperature be 25 DEG C extremely
It is uniformly mixed under the conditions of 45 DEG C and obtains treating fermentation material, then will treats that fermentation material loads feed fermentation bag；
5th step, be loaded into feed fermentation bag after fermentation material after temperature is 10 DEG C to 40 DEG C condition bottom fermentations 3 days to 30 days
Obtain mixed microorganism fermented feed.
7. employing mixed microorganism according to claim 6 degraded is gone mouldy aflatoxin production mixed microorganism in feed
The method of fermented feed, it is characterised in that in the first step, by 4 grams of sucrose, 4 grams of glucose, 4 grams of bean cake powders, 4g corn flour, 0.3g
Potassium dihydrogen phosphate is dissolved in 100 grams of physiological saline, obtains the nutrient solution that spreads cultivation, and the temperature for spreading cultivation is 34 DEG C to 36 DEG C.
8. the aflatoxin production mixing in feed of going mouldy of the employing mixed microorganism degraded according to claim 6 or 7 is micro-
The method of biological fermentation feed, it is characterised in that in second step, the bacterium amount of mixed bacterial reaches 1 × 1015Individual/more than g.
9. the employing mixed microorganism degraded according to claim 6 or 7 or 8 is gone mouldy aflatoxin production mixing in feed
The method of fermentative feedstuff of microbe, it is characterised in that in second step, bacterial classification growth accelerator be 30% to 40% FOS, 5% to
The mixture of 15% auxin and the ammonium hydrogen phosphate of surplus.
10. the employing mixed microorganism degraded according to claim 6 or 7 or 8 or 9 is gone mouldy aflatoxin production in feed
The method of mixed microorganism fermented feed, it is characterised in that in the 4th step, when after pending material process of going mouldy as chicken feed,
Pending feed powder of going mouldy is broken to into granularity less than less than 1.5 millimeters；As sheep feed or ox after pending material process of going mouldy
During feed, pending feed powder of going mouldy is broken to into granularity for 2.0 millimeters to 2.5 millimeters；Conduct after pending material process of going mouldy
During pig feed, pending feed powder of going mouldy is broken to into granularity for 1 millimeter to 1.3 millimeters.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107333967A (en) * | 2017-08-08 | 2017-11-10 | 李想 | It is a kind of to utilize the ageing raw grain and the fowl protein feed that just prepared by stalk and preparation method thereof of going mouldy |
| CN109452539A (en) * | 2018-11-14 | 2019-03-12 | 陈雨 | The biodegrading process of mycotoxin and less toxic fermentative feedstuff of microbe in corn and wheat and its converted products |
| CN109452451A (en) * | 2018-11-14 | 2019-03-12 | 陈雨 | The biodegrading process of mycotoxin and less toxic puffed soybean raw material in meal expeller and mealsolvent raw material |
| CN109452540A (en) * | 2018-11-14 | 2019-03-12 | 陈雨 | The biodegrading process of mycotoxin and less toxic fermentative feedstuff of microbe in oil plant seed and its converted products |
| CN112980657A (en) * | 2021-04-20 | 2021-06-18 | 湖南普菲克生物科技有限公司 | Method for preparing fermented feed by fermenting mildewed feed raw materials by combining bacteria, enzyme and prebiotics |
| CN113462589A (en) * | 2021-04-17 | 2021-10-01 | 苏州优利莱生物科技有限公司 | Leaven for degrading aflatoxin in feed and preparation method thereof |
| CN114287518A (en) * | 2021-12-27 | 2022-04-08 | 郑州云益生物科技有限公司 | Probiotic-containing licking brick for cattle and sheep and preparation method thereof |
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| CN109452539A (en) * | 2018-11-14 | 2019-03-12 | 陈雨 | The biodegrading process of mycotoxin and less toxic fermentative feedstuff of microbe in corn and wheat and its converted products |
| CN109452451A (en) * | 2018-11-14 | 2019-03-12 | 陈雨 | The biodegrading process of mycotoxin and less toxic puffed soybean raw material in meal expeller and mealsolvent raw material |
| CN109452540A (en) * | 2018-11-14 | 2019-03-12 | 陈雨 | The biodegrading process of mycotoxin and less toxic fermentative feedstuff of microbe in oil plant seed and its converted products |
| CN113462589A (en) * | 2021-04-17 | 2021-10-01 | 苏州优利莱生物科技有限公司 | Leaven for degrading aflatoxin in feed and preparation method thereof |
| CN112980657A (en) * | 2021-04-20 | 2021-06-18 | 湖南普菲克生物科技有限公司 | Method for preparing fermented feed by fermenting mildewed feed raw materials by combining bacteria, enzyme and prebiotics |
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Application publication date: 20170510 |