CN106614167A - Method for efficiently and quickly purifying escherichia coli in farmed oyster bodies - Google Patents
Method for efficiently and quickly purifying escherichia coli in farmed oyster bodies Download PDFInfo
- Publication number
- CN106614167A CN106614167A CN201710048947.3A CN201710048947A CN106614167A CN 106614167 A CN106614167 A CN 106614167A CN 201710048947 A CN201710048947 A CN 201710048947A CN 106614167 A CN106614167 A CN 106614167A
- Authority
- CN
- China
- Prior art keywords
- oyster
- seawater
- purification
- farmed
- escherichia coli
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000237502 Ostreidae Species 0.000 title claims abstract description 44
- 235000020636 oyster Nutrition 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 20
- 241000588724 Escherichia coli Species 0.000 title abstract description 11
- 239000013535 sea water Substances 0.000 claims abstract description 22
- JDLKFOPOAOFWQN-UHFFFAOYSA-N allicin Chemical compound C=CCSS(=O)CC=C JDLKFOPOAOFWQN-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000000746 purification Methods 0.000 claims abstract description 19
- JDLKFOPOAOFWQN-VIFPVBQESA-N Allicin Natural products C=CCS[S@](=O)CC=C JDLKFOPOAOFWQN-VIFPVBQESA-N 0.000 claims abstract description 18
- 235000010081 allicin Nutrition 0.000 claims abstract description 18
- 239000000645 desinfectant Substances 0.000 claims abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 15
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 235000015170 shellfish Nutrition 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 6
- 235000004611 garlic Nutrition 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 244000245420 ail Species 0.000 claims 1
- 230000000249 desinfective effect Effects 0.000 claims 1
- 230000000384 rearing effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 7
- 240000002234 Allium sativum Species 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 210000004872 soft tissue Anatomy 0.000 description 4
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000548230 Crassostrea angulata Species 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 210000002429 large intestine Anatomy 0.000 description 2
- 238000009364 mariculture Methods 0.000 description 2
- 238000006385 ozonation reaction Methods 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000000823 artificial membrane Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013332 fish product Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000010647 garlic oil Substances 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000002898 organic sulfur compounds Chemical class 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013613 poultry product Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to the field of marine cultivation and relates to a method for efficiently and quickly purifying escherichia coli in farmed oyster bodies. According to the method for efficiently and quickly purifying the escherichia coli in the farmed oyster bodies, allicin is utilized as a disinfectant, seawater is disinfected and the farmed oysters are put into the disinfected seawater for temporary rearing and purification. Compared with the prior art, the method has the advantages of being simple, low in economic cost and high in purification efficiency, and the method is natural, environmentally friendly, free of pollution and very good in practicability, and can be widely popularized and applied.
Description
Technical field
The invention belongs to field of marine aquaculture, is related to colibacillary innovative technology in a kind of purifying aquaculture oyster body.
Background technology
Allicin (allicin) is from the bulb (garlic) of Liliaceae allium garlic (Allium sativum)
A kind of organosulfur compound for extracting, scientific name is GIUCOSinoate (diallyl thiosulfinate), also known as
Garlic oil.Allicin is described as natural broad-spectrum antibiotic, 19th century Pasteur find that garlic has antibacterial activity first, hereafter
Research has shown that allicin has unique pharmacologically active, readily penetrates through immobilized artificial membrane and enters intracellular, with some retention compound phases
Interaction, realizes its pharmacological action.In addition, it also has antitumor, reduction cholesterol, platelet aggregation-against, protect liver, the prevention heart
The pharmacotoxicological effect such as vascular diseases, hypotensive, anti-oxidant.In recent years, both at home and abroad to the pharmacological research of allicin more and more extensively,
Deeply, the various preparations of allicin are in succession in clinical practice.
At present, allicin is widely used in the fields such as food and medicine.Allicin is also widely used for agricultural production
In, there is remarkable effect to improving livestock and poultry and fish products yield and quality.But allicin goes out to whether marine shellfish has
Bacterium ability has no always report.Shellfish purification concept comes from the Europe in 19th century, and shellfish purification technology is started from 1997 in China,
Start late.At present, various purification techniques are still in the exploratory stage, and the shellfish purification factory of commercial size is actually rare, many
Using purification methods such as ozonation system or ultraviolets.These methods have the disadvantages that：1. the time is long, generally require 48 hours with
On；2. high cost, no matter ozonation system or UV treatment are required for certain equipment；3. effect is undesirable, is extremely difficult to
National standard；4. quality shellfish is affected.
Oyster have the title of ocean " milk ", is cultured output highest shellfish in the world, and its meat flavour is delicious, nutrition is rich
Richness, economic worth is high.China is the big country of oyster culture first, but due to lacking the breeding environment of cleaning, it is interior on a large scale absolutely to support
It is also undisputable fact to grow E. CoIi content severe overweight in oyster body, so that the oyster culture of China is difficult outlet, shape
Into the situation that yield is high, the output value is low.
The content of the invention
The invention provides a kind of colibacillary method in efficient fast purification oyster culture body, can quickly solve existing
There is the exceeded problem of oyster culture Escherichia coli.
In order to reach the purpose of foregoing invention, employ the following technical solutions：Using allicin as disinfectant, seawater is entered
Row sterilization, is placed in oyster culture in the seawater of sterilization and temporarily support purification.By oyster cleaning, seawater sterilization, oyster temporarily support purification,
The processes such as E. coli detection, are purified oyster, reach country's even export standard.Comprise the following steps that：
S1 oysters are cleaned：Healthy oyster is selected, body surface attachment and dirt is removed, and is cleaned repeatedly with seawater；
S2 seawater is sterilized：Using allicin as disinfectant, disinfection concentration is 0.01-1.0g/L, and seawater is carried out disinfection, and is sterilized
Time is 2-6 hours；
S3 oysters temporarily support purification：The oyster for cleaning up is put into the seawater after sterilization temporarily foster 12-24 hours, oyster and water body
Weight ratio be 1：4-6.
Using National Standard Method-Escherichia coli MPN counting methods（GB4789.3—2010）, the oyster that will be purified through said method
E. coli detection is carried out, middle Escherichia coli quantity is controlled in 30-50 Escherichia coli/100g in the oyster body after detection purification
Soft tissue, is markedly inferior to national standard and EU criteria（Not more than 300/100g soft tissues）.
Compared with prior art, the present invention has advantages below：（1）Method is simple, easy to operate, practical.Only need to
Allicin is added in breeding seawater, without technical requirements, can large-scale application, easily popularization.（2）Economical and practical, low cost.
Allicin market is common, low price, only needs to 0.03-0.05 yuan per 100L seawater, low cost.（3）Used time is short, purify effect
Rate is high.Only need the even export standard that can be up to state standards for 12 hours, efficiency high.（4）The nuisanceless, feature of environmental protection is strong.Garlic
Element belongs to biologic product, environmentally safe, harmless, and the feature of environmental protection is strong.
Description of the drawings
Fig. 1 is the flow chart of present invention purification oyster.
Specific embodiment
Embodiment 1
Step is as follows：
（1）Oyster selects and cleans：The oyster of health is randomly selected from mariculture area（Portuguese oyster）120kg, cleans oyster
Surface sludge, and cleaned repeatedly with seawater, it is standby；
（2）Seawater is sterilized：The sterilization of 4 hours is carried out to 500L breeding seawaters with the allicin concentration of 0.05g/L；
（3）Oyster is temporarily supported：The oyster for cleaning up is put into the seawater after 500L sterilizations and is temporarily supported 12 hours；
（4）E. coli detection：30 temporarily foster oysters are selected at random, and solution takes its full tissue, grinding, mixes, is then divided into
10 equal portions, National Standard Method-Escherichia coli MPN counting methods（GB4789.3—2010）Large intestine bar in oyster body after detection purification
Bacterium number amount is 36/100g soft tissues, is markedly inferior to national standard and EU criteria（Not more than 300/100g software groups
Knit）.
Embodiment 2
Step is as follows：
（1）Oyster selects and cleans：The oyster of health is randomly selected from mariculture area（Portuguese oyster）100kg, cleans oyster
Surface sludge, and cleaned repeatedly with seawater, it is standby；
（2）Seawater is sterilized：The sterilization of 6 hours is carried out to 500L breeding seawaters with the allicin concentration of 0.03g/L；
（3）Oyster is temporarily supported：The oyster for cleaning up is put into the seawater after 500L sterilizations and is temporarily supported 12 hours；
（4）E. coli detection：30 temporarily foster oysters are selected at random, and solution takes its full tissue, grinding, mixes, and then takes 10
Equal portions, using National Standard Method-Escherichia coli MPN counting methods（GB4789.3—2010）Large intestine in oyster body after detection purification
Bacillus quantity is 40/100g soft tissues, is markedly inferior to national standard and EU criteria（Not more than 300/100g softwares
Tissue）.
Embodiment 1 and embodiment 2 illustrate, using the method for the present invention can efficiently, rapidly in purifying aquaculture oyster body
E. CoIi content.In addition, all, Escherichia coli are purified in shellfish body by allicin is also intention thought of the present invention
In one's power.
Claims (3)
1. a kind of colibacillary method in efficient fast purification oyster culture body, it is characterised in that methods described uses garlic
Element carries out disinfection as disinfectant to seawater, oyster culture is placed in the seawater of sterilization and temporarily support purification.
2. method according to claim 1, it is characterised in that comprise the following steps：
S1 selects the oyster of health, removes body surface attachment and dirt, and is cleaned repeatedly with seawater；
Using allicin as disinfectant, disinfection concentration is 0.01g/L-1.0g/L to S2, and seawater is carried out disinfection, and disinfecting time is 2-
6 hours；
The weight ratio that the oyster for cleaning up is put into the seawater after sterilization temporarily foster 12-24 hours, oyster and water body by S3 is 1：
4-6。
3. claim 1 methods described is used for the purification of marine shellfish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710048947.3A CN106614167B (en) | 2017-01-23 | 2017-01-23 | Method for rapidly purifying escherichia coli in cultured oysters |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710048947.3A CN106614167B (en) | 2017-01-23 | 2017-01-23 | Method for rapidly purifying escherichia coli in cultured oysters |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106614167A true CN106614167A (en) | 2017-05-10 |
| CN106614167B CN106614167B (en) | 2020-05-15 |
Family
ID=58840967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710048947.3A Active CN106614167B (en) | 2017-01-23 | 2017-01-23 | Method for rapidly purifying escherichia coli in cultured oysters |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106614167B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114731975A (en) * | 2022-04-08 | 2022-07-12 | 江苏海洋大学 | Method for improving survival rate of purified oysters at low cost |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023038522A1 (en) * | 2021-09-10 | 2023-03-16 | AHV International B.V. | Compositions and methods for treating biofilm disorders and infection |
-
2017
- 2017-01-23 CN CN201710048947.3A patent/CN106614167B/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114731975A (en) * | 2022-04-08 | 2022-07-12 | 江苏海洋大学 | Method for improving survival rate of purified oysters at low cost |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106614167B (en) | 2020-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hostins et al. | Managing input C/N ratio to reduce the risk of acute hepatopancreatic necrosis disease (AHPND) outbreaks in biofloc systems–a laboratory study | |
| CN100494093C (en) | Nourishing water conditioning agent for aquiculture | |
| CN103858819B (en) | The raising method of a kind of medicinal black-bone chicken | |
| CN105324481B (en) | Bacteriophage including its constituent and its application | |
| CN1298260C (en) | Sterilizing freshness preserving agent and its use | |
| CN107338198A (en) | A kind of Lactobacillus plantarum and its application | |
| CN102311929B (en) | Method for largely and rapidly reproducing lactic acid bacteria | |
| CN102389041B (en) | Chinese herbal feed additive for red swamp crayfish | |
| CN106614167A (en) | Method for efficiently and quickly purifying escherichia coli in farmed oyster bodies | |
| CN103766283A (en) | Microorganism promoted live pig breeding method | |
| CN101082038A (en) | Preparation of holothurian-benefit bacterium and method for restoring sea cucumber cultivation pool environment | |
| CN102090514A (en) | Compound immunopotentiator for left-eye and right-eye flounders and preparation method thereof | |
| CN102178296B (en) | Purple sweet potato drink and preparation process thereof | |
| CN106418003A (en) | Micro-ecological preparation combining water purification function for aquatic animals | |
| CN101891307A (en) | Marine culture biological cleanser and preparation method thereof | |
| KR20170143304A (en) | System for raising the shrimp having an excellent antiviral effect | |
| CN103966122A (en) | Providencia vermicola and application of providencia vermicola in preparing antibacterial drugs | |
| CN103387949A (en) | Scophthalmas maximus fin rot disease antagonistic bacterium and application thereof | |
| CN106852288B (en) | Poecilobdella manillensis culture method | |
| CN103241864B (en) | Novel rural sewage flocculent precipitate sterilizing agent and application thereof | |
| CN106337028A (en) | Cleaning agent, and preparation method and application thereof | |
| CN102038714B (en) | Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus | |
| CN105210975B (en) | The processing method in prawn Vibrio luminous disease pond | |
| KR100454228B1 (en) | A feed additive for cultured fish | |
| US5198114A (en) | Dolomitic activated carbon filter |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20170510 Assignee: Shantou Dafa Aquaculture Co.,Ltd. Assignor: SHANTOU University Contract record no.: X2023980047469 Denomination of invention: A rapid method for purifying Escherichia coli in cultured oysters Granted publication date: 20200515 License type: Common License Record date: 20231117 |
|
| EE01 | Entry into force of recordation of patent licensing contract |