CN106613939B - 用于高丛蓝莓体细胞胚间接再生的培养基及培养方法 - Google Patents
用于高丛蓝莓体细胞胚间接再生的培养基及培养方法 Download PDFInfo
- Publication number
- CN106613939B CN106613939B CN201610867326.3A CN201610867326A CN106613939B CN 106613939 B CN106613939 B CN 106613939B CN 201610867326 A CN201610867326 A CN 201610867326A CN 106613939 B CN106613939 B CN 106613939B
- Authority
- CN
- China
- Prior art keywords
- culture
- callus
- blueberry
- high clump
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 94
- 235000017537 Vaccinium myrtillus Nutrition 0.000 title claims abstract description 93
- 235000021014 blueberries Nutrition 0.000 title claims abstract description 93
- 240000000851 Vaccinium corymbosum Species 0.000 title claims abstract description 92
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 63
- 239000001963 growth medium Substances 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 30
- 230000000392 somatic effect Effects 0.000 title claims abstract description 24
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 96
- 230000004069 differentiation Effects 0.000 claims abstract description 35
- 230000035755 proliferation Effects 0.000 claims abstract description 32
- 239000002609 medium Substances 0.000 claims abstract description 31
- 230000006698 induction Effects 0.000 claims abstract description 20
- 241000196324 Embryophyta Species 0.000 claims abstract description 11
- 238000009630 liquid culture Methods 0.000 claims abstract description 11
- 239000007787 solid Substances 0.000 claims abstract description 8
- 238000005520 cutting process Methods 0.000 claims abstract description 4
- 238000000338 in vitro Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 18
- 230000006872 improvement Effects 0.000 claims description 16
- 229920001817 Agar Polymers 0.000 claims description 15
- 239000008272 agar Substances 0.000 claims description 15
- 239000005416 organic matter Substances 0.000 claims description 15
- 229930006000 Sucrose Natural products 0.000 claims description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 14
- 229960004793 sucrose Drugs 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 13
- 238000005286 illumination Methods 0.000 claims description 12
- 238000007711 solidification Methods 0.000 claims description 12
- 230000008023 solidification Effects 0.000 claims description 12
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 11
- 239000005720 sucrose Substances 0.000 claims description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 230000001965 increasing effect Effects 0.000 claims description 10
- 235000003642 hunger Nutrition 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 230000037351 starvation Effects 0.000 claims description 9
- 229920000742 Cotton Polymers 0.000 claims description 8
- GPXLRLUVLMHHIK-UHFFFAOYSA-N forchlorfenuron Chemical compound C1=NC(Cl)=CC(NC(=O)NC=2C=CC=CC=2)=C1 GPXLRLUVLMHHIK-UHFFFAOYSA-N 0.000 claims description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 6
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 239000007836 KH2PO4 Substances 0.000 claims description 6
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 229910052927 chalcanthite Inorganic materials 0.000 claims description 6
- 229910052564 epsomite Inorganic materials 0.000 claims description 6
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 239000003147 molecular marker Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000001968 nicotinic acid Nutrition 0.000 claims description 6
- 229960003512 nicotinic acid Drugs 0.000 claims description 6
- 239000011664 nicotinic acid Substances 0.000 claims description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 6
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 6
- 229960000344 thiamine hydrochloride Drugs 0.000 claims description 6
- 235000019190 thiamine hydrochloride Nutrition 0.000 claims description 6
- 239000011747 thiamine hydrochloride Substances 0.000 claims description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 6
- 239000011686 zinc sulphate Substances 0.000 claims description 6
- 239000004471 Glycine Substances 0.000 claims description 5
- 230000034303 cell budding Effects 0.000 claims description 5
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 4
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 4
- 229960000367 inositol Drugs 0.000 claims description 4
- 229910052603 melanterite Inorganic materials 0.000 claims description 4
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 4
- 244000060011 Cocos nucifera Species 0.000 claims description 3
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 235000013681 dietary sucrose Nutrition 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 244000077233 Vaccinium uliginosum Species 0.000 claims 2
- 230000000408 embryogenic effect Effects 0.000 abstract description 6
- 230000001939 inductive effect Effects 0.000 abstract description 5
- 230000032823 cell division Effects 0.000 abstract description 4
- 230000007774 longterm Effects 0.000 abstract description 4
- 239000011159 matrix material Substances 0.000 abstract description 2
- 230000008929 regeneration Effects 0.000 description 11
- 238000011069 regeneration method Methods 0.000 description 11
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 238000004113 cell culture Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- 240000000111 Saccharum officinarum Species 0.000 description 3
- 235000007201 Saccharum officinarum Nutrition 0.000 description 3
- 235000021028 berry Nutrition 0.000 description 3
- 210000002242 embryoid body Anatomy 0.000 description 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 2
- 244000283207 Indigofera tinctoria Species 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000032459 dedifferentiation Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 229940064880 inositol 100 mg Drugs 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- 239000010977 jade Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 2
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 2
- 229940023877 zeatin Drugs 0.000 description 2
- 235000019890 Amylum Nutrition 0.000 description 1
- DXIOVXYOEZZJPI-UHFFFAOYSA-N C1(=CC=CC=C1)NC(=O)N.N1C=CC=C1.[Cl] Chemical compound C1(=CC=CC=C1)NC(=O)N.N1C=CC=C1.[Cl] DXIOVXYOEZZJPI-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- HFCYZXMHUIHAQI-UHFFFAOYSA-N Thidiazuron Chemical compound C=1C=CC=CC=1NC(=O)NC1=CN=NS1 HFCYZXMHUIHAQI-UHFFFAOYSA-N 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610867326.3A CN106613939B (zh) | 2016-09-30 | 2016-09-30 | 用于高丛蓝莓体细胞胚间接再生的培养基及培养方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610867326.3A CN106613939B (zh) | 2016-09-30 | 2016-09-30 | 用于高丛蓝莓体细胞胚间接再生的培养基及培养方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106613939A CN106613939A (zh) | 2017-05-10 |
CN106613939B true CN106613939B (zh) | 2018-11-27 |
Family
ID=58853890
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610867326.3A Active CN106613939B (zh) | 2016-09-30 | 2016-09-30 | 用于高丛蓝莓体细胞胚间接再生的培养基及培养方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106613939B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108935088A (zh) * | 2018-09-17 | 2018-12-07 | 温州科技职业学院 | 一种提高蓝莓外植体辐射诱变率的方法 |
CN110771508B (zh) * | 2019-11-26 | 2022-07-05 | 大连大学 | 一种蓝莓人工种子制备方法 |
CN112931223B (zh) * | 2021-04-16 | 2022-05-31 | 山东大丰园农业有限公司 | 一种用于蓝莓组织培养的培养基及培养方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101838630A (zh) * | 2009-04-21 | 2010-09-22 | 山东高端蓝莓生物技术股份有限公司 | 一种高灌蓝莓组织培养复壮培养基制备方法 |
CN103181323A (zh) * | 2011-12-29 | 2013-07-03 | 洛阳理工学院 | 一种蓝莓胚状体培养方法 |
CN102577967A (zh) * | 2012-03-02 | 2012-07-18 | 湖州师范学院 | 一种高效的兔眼蓝莓组培快繁方法 |
CN103598093B (zh) * | 2013-10-31 | 2015-10-21 | 青岛文创科技有限公司 | 一种蓝莓胚状体的诱导方法 |
-
2016
- 2016-09-30 CN CN201610867326.3A patent/CN106613939B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN106613939A (zh) | 2017-05-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Ahmadian et al. | Micropropagation of carnation (Dianthus caryophyllus L.) in liquid medium by temporary immersion bioreactor in comparison with solid culture | |
CN105475130B (zh) | 一种红锥离体培养植株再生方法 | |
Lü et al. | Plant regeneration via somatic embryogenesis and shoot organogenesis from immature cotyledons of Camellia nitidissima Chi | |
CN101695283B (zh) | 文心兰花梗芽组培用的培养基和组培苗繁殖方法 | |
CN106613939B (zh) | 用于高丛蓝莓体细胞胚间接再生的培养基及培养方法 | |
CN102228003B (zh) | 一种羽衣甘蓝小孢子再生植株的培养方法 | |
CN104429952A (zh) | 一种培养结球甘蓝游离小孢子高效获得再生植株的方法 | |
CN102239803B (zh) | 一种青花菜小孢子再生植株的培养方法 | |
CN102134561B (zh) | 一种青天葵组培根状茎快速增殖的培养基 | |
Brackpool et al. | Regeneration in palms | |
CN113331059B (zh) | 一种以鸟王茶树胚轴为外植体建立高效再生体系的方法 | |
CN104737912A (zh) | 一种非洲菊组培快繁培养基及其培育方法 | |
CN107372122B (zh) | 橡胶树胚性愈伤组织诱导培养基及橡胶树胚性愈伤组织快速增殖方法 | |
CN103828716A (zh) | 少女石竹的组织培养方法 | |
CN109601387B (zh) | 以幼孢子囊群诱导的ggb途径的华南紫萁组培繁殖方法 | |
JPH038171B2 (zh) | ||
Ziv et al. | Morphogenic pattern of Nephrolepis exaltata cv. Bostoniensis in agar or liquid cultures: implications for micropropagation | |
CN107006367B (zh) | 一种‘阳光’樱组培快繁方法 | |
Hou et al. | Plant regeneration from protoplasts isolated from embryogenic calli of the forage legume Astragalus melilotoides Pall. | |
KR101636726B1 (ko) | 생물반응기 액체배양을 통한 배발생 캘러스 급속증식 및 고체 배지에서의 재분화를 통한 튤립 자구 대량생산 방법 | |
CN100382678C (zh) | 一种通过成熟胚愈伤诱导进行日本结缕草植株再生的方法及培养基 | |
CN103250641A (zh) | 甘蓝型油菜玻璃化再生苗的去玻璃化方法 | |
CN107022519A (zh) | 茶叶悬浮单细胞的分离培养方法 | |
CN108782244B (zh) | 一种龙珠果组织培养方法 | |
CN102845303A (zh) | 一种扩大普陀鹅耳枥种群数量的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20181012 Address after: 325026 Wenzhou City National University Science Park incubator, No. 38 Dongfang South Road, Ouhai District, Wenzhou, Zhejiang Applicant after: Wenzhou Vocational College of Science and Technology Applicant after: Wenzhou Institute of Agricultural Sciences Address before: 325006 No. 1000 Hongqiao Road, Ouhai District, Wenzhou, Zhejiang, 1000 Applicant before: Wenzhou Vocational College of Science and Technology |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20190102 Address after: 325026 Wenzhou City National University Science Park incubator, No. 38 Dongfang South Road, Ouhai District, Wenzhou, Zhejiang Patentee after: Wenzhou Vocational College of Science and Technology Address before: 325026 Wenzhou City National University Science Park incubator, No. 38 Dongfang South Road, Ouhai District, Wenzhou, Zhejiang Co-patentee before: Wenzhou Institute of Agricultural Sciences Patentee before: Wenzhou Vocational College of Science and Technology |
|
TR01 | Transfer of patent right |