CN106581364A - Essential oil composition and applications thereof in preparing preparations capable of alleviating anxiety - Google Patents
Essential oil composition and applications thereof in preparing preparations capable of alleviating anxiety Download PDFInfo
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- CN106581364A CN106581364A CN201611199924.4A CN201611199924A CN106581364A CN 106581364 A CN106581364 A CN 106581364A CN 201611199924 A CN201611199924 A CN 201611199924A CN 106581364 A CN106581364 A CN 106581364A
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- 239000000341 volatile oil Substances 0.000 title claims abstract description 73
- 208000019901 Anxiety disease Diseases 0.000 title claims abstract description 38
- 230000036506 anxiety Effects 0.000 title claims abstract description 37
- 239000000203 mixture Substances 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 240000000560 Citrus x paradisi Species 0.000 claims abstract description 30
- 239000003921 oil Substances 0.000 claims description 123
- 241000220317 Rosa Species 0.000 claims description 22
- 241000628997 Flos Species 0.000 claims 7
- 230000000694 effects Effects 0.000 abstract description 22
- 238000002474 experimental method Methods 0.000 abstract description 9
- 239000003814 drug Substances 0.000 abstract description 6
- 240000006066 Rosa rugosa Species 0.000 abstract description 2
- 235000000659 Rosa rugosa Nutrition 0.000 abstract description 2
- 230000036541 health Effects 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 description 37
- 244000251953 Agaricus brunnescens Species 0.000 description 29
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 29
- 241001465754 Metazoa Species 0.000 description 25
- 238000002347 injection Methods 0.000 description 24
- 239000007924 injection Substances 0.000 description 24
- 238000012360 testing method Methods 0.000 description 21
- 241000699666 Mus <mouse, genus> Species 0.000 description 20
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 239000003205 fragrance Substances 0.000 description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 230000035943 smell Effects 0.000 description 12
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 11
- 229920000053 polysorbate 80 Polymers 0.000 description 11
- 230000002159 abnormal effect Effects 0.000 description 9
- 238000013329 compounding Methods 0.000 description 9
- 239000013641 positive control Substances 0.000 description 9
- 150000001875 compounds Chemical class 0.000 description 8
- AAOVKJBEBIDNHE-UHFFFAOYSA-N diazepam Chemical compound N=1CC(=O)N(C)C2=CC=C(Cl)C=C2C=1C1=CC=CC=C1 AAOVKJBEBIDNHE-UHFFFAOYSA-N 0.000 description 8
- 229960003529 diazepam Drugs 0.000 description 8
- 230000000384 rearing effect Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 230000037396 body weight Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 239000002304 perfume Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000001953 sensory effect Effects 0.000 description 3
- 230000000391 smoking effect Effects 0.000 description 3
- 235000013599 spices Nutrition 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000008451 emotion Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 241001290610 Abildgaardia Species 0.000 description 1
- 241000209495 Acorus Species 0.000 description 1
- 241000544682 Acorus tatarinowii Species 0.000 description 1
- 206010002869 Anxiety symptoms Diseases 0.000 description 1
- 241000209524 Araceae Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 244000075634 Cyperus rotundus Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 101150118484 Macf1 gene Proteins 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000010779 crude oil Substances 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000004247 hand Anatomy 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
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- 239000003960 organic solvent Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/89—Cyperaceae (Sedge family)
- A61K36/8905—Cyperus (flatsedge)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
Abstract
The invention relates to the technical field of traditional Chinese medicine health care, and in particular relates to an essential oil composition and applications of the essential oil composition in preparing preparations capable of alleviating anxiety. The essential oil composition provided by the invention contains rhizoma cyperi essential oil, rhizoma acori graminei essential oil, grapefruit essential oil and rosa rugosa Kushui essential oil. The experiment proves that the four kinds of essential oil have the certain efficacy of alleviating anxiety after being smelled and sucked, after the four kinds of essential oil are compounded, the synergistic effect can be generated, and the effect of alleviating anxiety can be obviously improved (p is smaller than 0.05). The preparation capable of alleviating anxiety prepared from the essential oil composition has the advantage of being safe and convenient to use, and the smell can be easily accepted.
Description
Technical field
The present invention relates to health protection with Chinese medicine technical field, more particularly to a kind of essential oil composition and its alleviate anxiety preparing
Preparation in application.
Background technology
Anxiety is a kind of nervous unhappy emotion of heart common in life and work, is prevalent in normal person
In, and in the crowd that some bodies are felt under the weather, such as in the crowd with disease, the ratio with anxiety is more
It is high.The anxiety or even anxiety neurosis of these crowds may improve its incidence probability.Simultaneously anxiety can bring other problems, such as
Insomnia etc., these emotion class diseases interact and belong to the disease of easy recurrence, let go unchecked and easily aggravate one's illness, in some instances it may even be possible to send out
Open up as depression.
At present treatment anxiety is mainly by taking based on Western medicine, although the problem for existing be several generations medicine progressively improving,
But still have side effect strong, the problems such as there is drug resistance after treatment several weeks, therefore a kind of new therapy is found replacing or auxiliary
Original pharmacotherapy is helped to become the target that people try to explore.
Possess the conventional fragrant therapy up to the usage history of thousand and become and be endowed greatly desired auxiliary treatment handss
Section.Developed country just had begun to attempt using aromatotherapy between the treatment of these diseases, last century Mo, land before 30 years
Continue the scientific research for having researcher to begin to focus on this respect, these researchs are from animal experiment and small-scale human trial angle
Aromatotherapy provides certain theoretical foundation, and either compound recipe be used to alleviating or treat anxiety and ask also some quintessence oil folk prescriptions
Topic, but its formula is typically more complicated, and raw material is difficult to obtain.Using theory of Chinese medical science, the quintessence oil to extracting in Chinese crude drug is carried out
Compounding, to realize that the research for alleviating anxiety symptom should be taken seriously.
The content of the invention
In view of this, the technical problem to be solved in the present invention is to provide a kind of essential oil composition and its alleviate Jiao preparing
Application in the preparation of worry.The essential oil composition that the present invention is provided can play a part of to alleviate anxiety well, and its effect is excellent
In a kind of quintessence oil of exclusive use.
The essential oil composition that the present invention is provided, including Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances essence
Oil.
Rhizoma Cyperi quintessence oil of the present invention is the dry root stem to sedge dried tuber (Cyperus rotundus L.)
Divide and extract the quintessence oil for obtaining.Rhizoma Acori Graminei quintessence oil is to Acorus of Araceae plant Rhizoma Acori Graminei (Acorus tatarinowii
Schott the root stock that is dried) extracts the quintessence oil for obtaining.Grapefruit essential oil is to rutaceae grapefruit (Citrus
Paradise Macf.) fresh peel extract obtain quintessence oil.Rose grievances quintessence oil is to rosaceous plant Rose grievances
The flower of (Rosa rugosa ' kushui ') extracts the quintessence oil for obtaining.
In order to improve the safety for using, reduce the probability that side effect occurs, extraction of essential oil mistake used in the present invention
Journey does not use organic solvent.Preferably, the extracting method of Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil and Rose grievances quintessence oil is vapor
The way of distillation；Extraction to grapefruit essential oil adopts cold-press.
Experiment shows that Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil all have by smelling to inhale
Four compoundings can be produced potentiation by effect of certain alleviation anxiety, significantly improve the effect (p for alleviating anxiety<
0.05).And after the present invention is by various quintessence oils compounding, while ensure that the effect for alleviating anxiety, sensory effects are improve, make
Most people can receive the abnormal smells from the patient for compounding quintessence oil.
In the essential oil composition that the present invention is provided, Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil
Volume ratio be (30～50)：(20～40)：(10～30)：(1～20).
In the preferable compositionss of effect, the volume of Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil
Than for (35～45)：(25～35)：(15～25)：(5～15).
In some embodiments, the volume ratio of Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil is
40：30：20：10.
In some embodiments, the volume ratio of Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil is
35：35：15：15.
In some embodiments, the volume ratio of Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil is
42：33：15：10.
In some embodiments, the volume ratio of Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil is
50：40：30：20.
In some embodiments, the volume ratio of Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil is
30：20：10：1.
In some embodiments, the volume ratio of Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil is
45：25：25：5.
Experiment shows that Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil are enough carried by smelling energy-absorbing
High mice accounts for the ratio into open arms and closure arm total degree into the open arms number of times of Elevated plus-maze, and raising is being opened
The ratio that the arm time of staying accounts for total time is put, therefore is illustrated, the essential oil composition that the present invention is provided there can be certain alleviation
Four compoundings can be produced potentiation by effect of anxiety, significantly improve the effect (p for alleviating anxiety<0.05).And investigation knot
Fruit shows that the essential oil composition that the present invention is provided can have good organoleptic attribute, and its abnormal smells from the patient can be liked by people.
The present invention provide essential oil composition preparation method be by Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and
Rose grievances quintessence oil mixes.
Application of the essential oil composition that the present invention is provided in the preparation for alleviating anxiety is prepared.
Present invention also offers a kind of preparation for alleviating anxiety, including the essential oil composition that the present invention is provided.
The preparation that the present invention is provided directly affects big by quintessence oil small molecular active volatile composition by olfactory system
Cerebral nervous system, so as to have functions that to alleviate anxiety, it is to avoid the side effect that oral western medicine is brought, using safety, uses
Method is also more convenient.
The preparation for alleviating anxiety of the present invention can only include the essential oil composition that the present invention is provided, and can also carry the present invention
For compositionss add appropriate adjuvant and make dosage form easy to use, for example：Capsule, drop etc..
In alleviating the preparation of anxiety, the volume fraction of the essential oil composition that the present invention is provided is 1%～25%.
In alleviating the preparation of anxiety, also including substrate；Substrate is the mixture of water and tween 80.
In substrate, water is 3 with the volume ratio of tween 80:1.
Present invention also offers a kind of method for alleviating anxiety, the method inhales the preparation that provides of the present invention to smell.
The present invention provide alleviations anxiety method smell suction time be 2min～10min.
Preferably, the time for smelling suction is 5min.
Preferably, the temperature that essential oil composition is heated when smelling suction is 80 DEG C ± 10 DEG C.
In the essential oil composition that the present invention is provided, including Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances
Quintessence oil, experiment shows that these four quintessence oils all have functions that certain alleviation anxiety by smelling suction, and four compoundings can be produced
Potentiation, significantly improves the effect (p for alleviating anxiety<0.05).Additionally, essential oil composition of the present invention its abnormal smells from the patient receives
Degree is high, is made into the preparation of alleviation anxiety and can have the advantages that using conveniently, safely.Also, the present invention is by various quintessence oils
After compounding, while ensure that the effect for alleviating anxiety, sensory effects are improve, make most people to receive compounding essence
The abnormal smells from the patient of oil.
Specific embodiment
The invention provides a kind of essential oil composition and its application in the preparation for alleviating anxiety is prepared, art technology
Personnel can use for reference present disclosure, be suitably modified technological parameter realization.Specifically, all similar replacements and change
Dynamic apparent to those skilled in the art, they are considered as being included in the present invention.The method of the present invention and should
With being described by preferred embodiment, related personnel substantially can be in without departing from present invention, spirit and scope
Methods herein and application are modified or the technology of the present invention is realized and applied to suitably change with combining.
With reference to embodiment, the present invention is expanded on further：
Embodiment 1～6
Essential oil composition is prepared by the prescription of table 1：
Table 1：Embodiment 1～6
| Rhizoma Cyperi quintessence oil | Rhizoma Acori Graminei quintessence oil | Grapefruit essential oil | Rose grievances quintessence oil | |
| Embodiment 1 | 400μL | 300μL | 200μL | 100μL |
| Embodiment 2 | 350μL | 350μL | 150μL | 150μL |
| Embodiment 3 | 420μL | 330μL | 150μL | 100μL |
| Embodiment 4 | 500μL | 400μL | 300μL | 200μL |
| Embodiment 5 | 300μL | 200μL | 100μL | 10μL |
| Embodiment 6 | 450μL | 250μL | 250μL | 50μL |
Healthy adult male mouse of kunming with 5 week old as experimental subject, 25 ± 5g of body weight, sub-cage rearing, per 5, cage,
20 ± 1 DEG C of rearing conditions, humidity 55% ± 5%, light and shade cycle 12h (07:00 turns on light)；By Mouse feeder more than 5 days before experiment,
Mice is set to adapt to environment.
160 mices are randomly divided into into 20 groups, 8 per group, respectively blank group, positive control group and quintessence oil test group 1,2,
3。
Blank group:Water:Tween 80 (v/v) is 3:1 mixes, and takes the champignon that 2ml is added to the animal champignon room for covering lid
In lamp, Fragrance Lamp is opened, preheat 5min.Mouse peritoneal injection normal saline (10ml/kg), is placed on mice after the completion of injection
In animal champignon room, close the lid, taking out mice after 5min carries out Elevated plus-maze test.
Positive control group:Water:Tween 80 (v/v) is 3:1 mixes, and takes the perfume (or spice) that 2ml is added to the animal champignon room for covering lid
In smoking lamp, Fragrance Lamp is opened, preheat 5min.Normal saline (normal saline consumption 10ml/ of the mouse peritoneal injection containing diazepam
Kg, the consumption of diazepam is 2.5mg/kg), mice is placed in animal champignon room after the completion of injection, close the lid, after 5min
Mice is taken out carries out Elevated plus-maze test.
Quintessence oil test group：Water:Tween 80 (v/v) is 3:1 mixes, and takes 2ml and is added to the animal champignon room for covering lid
In Fragrance Lamp, 20 μ L, 100 μ L, the compound essential Oil of 500 μ L (being designated as quintessence oil test group 1～3 successively) are added, open Fragrance Lamp, in advance
Hot 5min.Mouse peritoneal injection normal saline (10ml/kg), is placed on mice in animal champignon room after the completion of injection, covers
Lid, taking out mice after 5min carries out Elevated plus-maze test.
Statistics mice accounts for the ratio into open arms and closure arm total degree into the open arms number of times of Elevated plus-maze
(being designated as number of times ratio) and the ratio (being designated as time ratio) of total time is accounted in the open arms time of staying, its is weighed as index burnt
Worry degree.As a result such as table 2：
The quintessence oil effect detection of table 2
Embodiment 7
The quintessence oil of the formula of Example 1～6 is examination material.Healthy adult male mouse of kunming with 5 week old as experimental subject,
25 ± 5g of body weight, sub-cage rearing, per 5, cage, 20 ± 1 DEG C of rearing conditions, humidity 55% ± 5%, light and shade cycle 12h (07:00 opens
Lamp)；By Mouse feeder more than 5 days before experiment, mice is set to adapt to environment.
112 mices are randomly divided into into 14 groups, 8 per group, respectively blank group, positive control group and quintessence oil test group a, b.
Blank group:Water:Tween 80 (v/v) is 3:1 mixes, and takes the champignon that 2ml is added to the animal champignon room for covering lid
In lamp, Fragrance Lamp is opened, preheat 5min.Mouse peritoneal injection normal saline (10ml/kg), is placed on mice after the completion of injection
In animal champignon room, close the lid, taking out mice after 5min carries out Elevated plus-maze test.
Positive control group:Water:Tween 80 (v/v) is 3:1 mixes, and takes the perfume (or spice) that 2ml is added to the animal champignon room for covering lid
In smoking lamp, Fragrance Lamp is opened, preheat 5min.Normal saline (normal saline consumption 10ml/ of the mouse peritoneal injection containing diazepam
Kg, the consumption of diazepam is 2.5mg/kg), mice is placed in animal champignon room after the completion of injection, close the lid, after 5min
Mice is taken out carries out Elevated plus-maze test.
Quintessence oil test group：Water:Tween 80 (v/v) is 3:1 mixes, and takes 2ml and is added to the animal champignon room for covering lid
In Fragrance Lamp, the compound essential Oil of 100 μ L is added, open Fragrance Lamp, preheat 5min.Mouse peritoneal injection normal saline (10ml/
Kg), mice is placed in animal champignon room after the completion of injection, is closed the lid, will after 2min, 10min (quintessence oil test group a, b)
Mice is taken out and carries out Elevated plus-maze test.
Statistics mice accounts for the ratio into open arms and closure arm total degree into the open arms number of times of Elevated plus-maze
(being designated as number of times ratio) and the ratio (being designated as time ratio) of total time is accounted in the open arms time of staying, its is weighed as index burnt
Worry degree.As a result such as table 3：
Table:3 quintessence oil effect detection
Embodiment 8
Healthy adult male mouse of kunming with 5 week old as experimental subject, 25 ± 5g of body weight, sub-cage rearing, per 5, cage,
20 ± 1 DEG C of rearing conditions, humidity 55% ± 5%, light and shade cycle 12h (07:00 turns on light)；By Mouse feeder more than 5 days before experiment,
Mice is set to adapt to environment.
64 mices are randomly divided into into 8 groups, 8 per group, respectively blank group, positive control group and quintessence oil test group 1,2,3.
Blank group:Take 2ml pure water to be added in the Fragrance Lamp of the animal champignon room for covering lid, open Fragrance Lamp, in advance
Hot 5min.Mouse peritoneal injection normal saline (10ml/kg), is placed on mice in animal champignon room after the completion of injection, covers
Lid, taking out mice after 5min carries out Elevated plus-maze test.
Positive control group:Take 2ml pure water to be added in the Fragrance Lamp of the animal champignon room for covering lid, open Fragrance Lamp,
Preheating 5min.(normal saline consumption 10ml/kg, the consumption of diazepam is normal saline of the mouse peritoneal injection containing diazepam
2.5mg/kg), mice is placed in animal champignon room after the completion of injection, is closed the lid, taking out mice after 5min carries out height
Frame plus maze is tested.
Quintessence oil test group：Take 2ml pure water to be added in the Fragrance Lamp of the animal champignon room for covering lid, open champignon
Lamp, preheats 5min, is separately added into the compound essential Oil of 100 μ L embodiments 1～6.Mouse peritoneal injection normal saline (10ml/kg),
Mice is placed in animal champignon room after the completion of injection, is closed the lid, taking out mice after 5min carries out Elevated plus-maze
Test.
Statistics mice accounts for the ratio into open arms and closure arm total degree into the open arms number of times of Elevated plus-maze
(being designated as number of times ratio) and the ratio (being designated as time ratio) of total time is accounted in the open arms time of staying, its is weighed as index burnt
Worry degree.As a result as in table 4：
The quintessence oil effect detection of table 4：
Comparative example 1
Respectively with Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances quintessence oil quintessence oil to try material.
Healthy adult male mouse of kunming with 5 week old as experimental subject, 25 ± 5g of body weight, sub-cage rearing, per 5, cage,
20 ± 1 DEG C of rearing conditions, humidity 55% ± 5%, light and shade cycle 12h (07:00 turns on light)；By Mouse feeder more than 5 days before experiment,
Mice is set to adapt to environment.
48 mices are randomly divided into into 6 groups, 8 per group, respectively blank group, positive control group and quintessence oil test group.
Blank group：Water:Tween 80 (v/v) is 3:1 mixes, and takes the champignon that 2ml is added to the animal champignon room for covering lid
In lamp, Fragrance Lamp is opened, preheat 5min.Mouse peritoneal injection normal saline (10ml/kg), is placed on mice after the completion of injection
In animal champignon room, close the lid, taking out mice after 5min carries out Elevated plus-maze test.
Positive control group：Water:Tween 80 (v/v) is 3:1 mixes, and takes the perfume (or spice) that 2ml is added to the animal champignon room for covering lid
In smoking lamp, Fragrance Lamp is opened, preheat 5min.Normal saline (normal saline consumption 10ml/ of the mouse peritoneal injection containing diazepam
Kg, the consumption of diazepam is 2.5mg/kg), mice is placed in animal champignon room after the completion of injection, close the lid, after 5min
Mice is taken out carries out Elevated plus-maze test.
Quintessence oil test group：Water:Tween 80 (v/v) is 3:1 mixes, and takes 2ml and is added to the animal champignon room for covering lid
In Fragrance Lamp, Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and the Rose grievances quintessence oil of 100 μ L are separately added into, open champignon
Lamp, preheats 5min.Mouse peritoneal injection normal saline (10ml/kg), is placed on mice in animal champignon room after the completion of injection,
Close the lid to take out mice after 5min and carry out Elevated plus-maze test.
Statistics mice accounts for the ratio into open arms and closure arm total degree into the open arms number of times of Elevated plus-maze
(being designated as number of times ratio) and the ratio (being designated as time ratio) of total time is accounted in the open arms time of staying, its is weighed as index burnt
Worry degree.As a result such as table 5：
The quintessence oil effect detection of table 5
| It is blank | Positive control group | Rhizoma Cyperi quintessence oil | Rhizoma Acori Graminei quintessence oil | Grapefruit essential oil | Rose grievances quintessence oil | |
| Number of times ratio (%) | 1.2 | 12.8 | 5.2 | 4.7 | 2.9 | 6.0 |
| Time ratio (%) | 0.91 | 12.1 | 5.7 | 4.9 | 2.7 | 5.5 |
The effect of the compound essential Oil that the effect that each quintessence oil is used alone is prepared with embodiment 1～6 does statistical analysis, ties
Fruit shows：Effect is significant of the compound essential Oil that embodiment 1～6 is provided in terms of anxiety is alleviated is used alone (p better than each quintessence oil<
0.05)。
The abnormal smells from the patient acceptance of embodiment 9 is tested
Method of testing：
A small amount of different types of quintessence oil is picked with the fragrant paper of examination, allows volunteer to smell suction, then evaluation is made to the abnormal smells from the patient of quintessence oil,
Respectively like, it is general and do not like.
Volunteer 100 is randomly selected, as stated above respectively to the compound recipe crude oil and Rhizoma Cyperi quintessence oil, stone of embodiment 1～6
Rhizoma Acori Graminei quintessence oil, grapefruit essential oil, Rose grievances quintessence oil are made an appraisal, and statistical result, table is listed in Table 6 below：
The abnormal smells from the patient acceptance evaluation of table 6 is counted
As a result show, most of volunteer thinks that Rhizoma Acori Graminei quintessence oil or Rhizoma Cyperi quintessence oil abnormal smells from the patient be not good in crowd.But Rhizoma Acori Graminei
Quintessence oil and Rhizoma Cyperi quintessence oil are splendid to the remission effect of anxiety.After embodiment 1～6 is by various quintessence oils compounding, alleviation is being ensure that
While the effect of anxiety, sensory effects are improve, make most people to receive to compound the abnormal smells from the patient of quintessence oil.
The above is only the preferred embodiment of the present invention, it is noted that for those skilled in the art come
Say, under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (10)
1. a kind of essential oil composition, it is characterised in that including Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and Rose grievances
Quintessence oil.
2. essential oil composition according to claim 1, the Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and misery
The volume ratio of Flos Rosae Rugosae quintessence oil is (30～50)：(20～40)：(10～30)：(1～20).
3. essential oil composition according to claim 1, the Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and misery
The volume ratio of Flos Rosae Rugosae quintessence oil is 40：30：20：10.
4. essential oil composition according to claim 1, the Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and misery
The volume ratio of Flos Rosae Rugosae quintessence oil is 35：35：15：15.
5. essential oil composition according to claim 1, the Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and misery
The volume ratio of Flos Rosae Rugosae quintessence oil is 42：33：15：10.
6. essential oil composition according to claim 1, the Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and misery
The volume ratio of Flos Rosae Rugosae quintessence oil is 50：40：30：20.
7. essential oil composition according to claim 1, the Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and misery
The volume ratio of Flos Rosae Rugosae quintessence oil is 30：20：10：1.
8. essential oil composition according to claim 1, the Rhizoma Cyperi quintessence oil, Rhizoma Acori Graminei quintessence oil, grapefruit essential oil and misery
The volume ratio of Flos Rosae Rugosae quintessence oil is 45：25：25：5.
9. application of the essential oil composition of any one of claim 1～8 in the preparation for alleviating anxiety is prepared.
10. it is a kind of alleviate anxiety preparation, it is characterised in that including the essential oil composition described in any one of claim 1～8.
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| CN109157572A (en) * | 2018-09-07 | 2019-01-08 | 上海交通大学 | The application of Rosa Damascana prevention and treatment anxiety disorder |
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