CN102247479A - Antitumor strong medicine and preparation method thereof - Google Patents
Antitumor strong medicine and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an antitumor strong medicine, which is prepared from barbed skullcap herb, oldenlandia diffusa, berchemia lineate and the like and can be added with filler or solution which is in accordance with medicinal standards to form solid preparation or liquid preparation in pharmacy. The antitumor strong medicine applies tonification and purgation in combination, addresses both root causes and symptoms, removes swelling and stasis, conducts the functions of tonifying yin and blood, restores pulse by tonifying qi and regulates immune system of the human body, and has effects of resisting tumor, keeping smoothness of detoxification channels and suppressing the growth of cancer cells.
Description
Technical field
The present invention relates to a kind of strong medicine preparation that suppresses tumor and preparation method thereof.
Background technology
Malignant tumor is the principal disease of harm humans health and lives.Estimated that the whole world had the people about 5,000,000 to die from malignant tumor every year approximately in 1976 according to World Health Organization (WHO).In recent decades, because transmissions of disease such as the continuous deterioration of ecological environment, acquired immune deficiency syndrome (AIDS), the sickness rate of malignant tumor is in rising trend, and becomes the second largest deadly cause of disease after cardiovascular disease.At present, the tumor treatment means are had marked improvement, but treatment the most serious, that account for the solid tumor of malignant tumor more than 90% still fails to reach gratifying effect to the harm humans life and health.
Tumor is the body tissue cell, under different carcinogenic factor long terms, take place a series of over-drastic, inharmonic hypertrophy and unusual differentiation and the pathological changes that forms after even carcinogenic factor fails, still continues undue growth.Its profile often shows as lump, is generally optimum and pernicious two kinds.Because the cause of disease of tumor is various, the sick kind differed, and the clinical manifestation multiterminal so pathological change also is very complicated, are not isolated and single.Modern TCM is thought, the nearly imbalance of YIN and YANG of pathogenesis of malignant tumor (cancer), stagnation of QI blood are crazy about, the hemorrhoid knot is wet poly-, cause accumulate in the pyretic toxicity, deficiency of qi and blood etc., particularly in today of industrial civilization prosperity, people are constantly in the face of various ambient pressures, environmental pollution, water, air and chemical fertilizer, pesticide, vehicle exhaust, food additive, unhygienic diet and often ediblely pickle food, the smoking of fire-cureing, drink, factor affecting such as noise jamming and atmosphere radioactive substance.These various heterotoxins enter people's body, health risk by different approaches, and the disorder of body metabolic function, extremely easily the various refuses that produce in the metabolism are piled up and be stuck in the metabolism pipeline, stop up normal drain passage, constitute the endogenous toxin, bring out human body and produce various condition of illness.Finally can cause the various dysfunctions of body, as the endocrine disturbance, degradation under lipid metabolism imbalance, the function of immune system causes the generation of tumor.Therefore, the oncology is the emphasis of medical research always, seeks effective antitumour medicine and method, and thoroughly capturing tumor is the important subject of world medical circle.Studies show that many Chinese medicines have antitumor action, especially compound recipe has multiple action component and produces antitumor action by multiple action pathway.Some medical facility and medical personnel have delivered the more open source literature report that utilizes Chinese herbal treatment to suppress tumor in recent years, for example:
1, Chinese patent, application number: 02131252.4 applying date: 2002.09.19 title: a kind of Chinese medicine preparation for the treatment of malignant tumor and preparation method thereof, open (bulletin) number: CN1483423, open (bulletin) day: 2004.03.24, application (patent right) people: Yu Tiande, address: No. 57 Liaoning, total stop, peace zone, ShenYang, Liaoning Province city ten thousand morals medicine company limited, invention (design) people: Yu Tiande, summary: a kind of Chinese medicine preparation for the treatment of malignant tumor and preparation method thereof, this Chinese medicine preparation is with Herba Hedyotidis Diffusae, Herba Sarcandrae, Herba Scutellariae Barbatae, Rhizoma Corydalis, rhizoma sparganic, Rhizoma Curcumae, Radix Salviae Miltiorrhizae, Radix Ginseng, the Radix Astragali, Ganoderma, Caulis Spatholobi, the Radix Rehmanniae, Fructus Lycii, Radix Trichosanthis, Herba Taraxaci, Radix Sophorae Tonkinensis, Radix Sophorae Flavescentis, Radix Glycyrrhizae is a primary raw material, be prepared from by following steps: raw material is prepared, the Chinese medicine preparation preparation, finished product processing, packing, check.The result who treats cancer drug according to clinical use the present invention shows: this medicine all has better curative effect to various tumors.The a few days sees that promptly tumor patient tumor body obviously dwindles after taking medicine.To advanced carcinoma is painful the persistency analgesic effect arranged.All react invariably and toxic and side effects after various cancer patients take medicine, all can reach the effect of relief of symptoms life-saving.
2, Chinese patent, application number: 200910019337.6 applyings date: 2009.10.15 title: a kind of antineoplastic Chinese medicine composition, open (bulletin) number: CN101664536, open (bulletin) day: 2010.03.10, application (patent right) people: Zhao Jinzhong, address: invention (design) people: the Zhao Jinzhong of Shandong Province Liaocheng City Linqing City institute of traditional Chinese medicine; Zhao Mingyang, summary: a kind of antineoplastic Chinese medicine composition.It is mainly made by crude drug such as Radix Bupleuri, Radix Curcumae, Rhizoma Cyperi, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae, Cortex Moutan, Radix Paeoniae Rubra, Radix Ginseng, the Rhizoma Atractylodis Macrocephalae, the Radix Astragali, Radix Rehmanniae, Herba Scutellariae Barbatae, Herba Hedyotidis Diffusae, Herba Taraxacis.It can use the treatment tumor separately, also can use simultaneously with operation, radiotherapy, chemotherapy, be characterized in instant effect, curative effect is reliable, not finding has apparent side effect, both can control growth of tumor in a short time, eliminates tumor, can also significantly strengthen human body constitution simultaneously, improve himself disease-resistant repair ability.It is a kind of desirable antitumor drug that more produces effect and be free from side effects than operation, chemotherapy, radiotherapy.
3, Chinese patent, application number: 200410013488.8 applyings date: 2004.07.19, title: a kind of Chinese medicine preparation for the treatment of cancer and preparation method thereof, open (bulletin) number: CN1596936, open (bulletin) day: 2005.03.23 applies for (patent right) people: Xing Ledao, address: the People's Hospital, Tuanfeng County, Hubei Province medicine section building, invention (design) people: Xing Ledao; Huang Shuoyan, summary: a kind of Chinese medicine preparation for the treatment of cancer and preparation method thereof, relate to a kind of treatment cancer, comprise the brain cancer, Chinese medicine preparation of cancer of pancreas etc. and preparation method thereof.Chinese medicine preparation by sirikaya total lactones, Rhizoma Paridis extract, Herba Salviae Chinensis extract, Herba Hedyotidis Diffusae extract, Herba Scutellariae Barbatae extract, Radix phytolaccae extract, Rabdosia rubescens extract, Rhizoma Polygoni Cuspidati extract, Herba Agrimoniae extract, Semen Ricini, Semen Persicae extract, Rhizoma Curcumae Longae extract etc. through pulverizing, make the solvent low-temperature extraction with carbon dioxide, reclaim, concentrate, isolate effective ingredient, mix, through the carbon dioxide pelletize of dusting, embedding gets product.The present invention combines the natural drug sirikaya total lactones by modern science technology with Chinese medicine, Chinese medicine extract, used the nanometer embedding techniques, has improved curative effect.It can replace chemotherapeutics, malignant tumor tumor body is dwindled or disappears, and prevents the recurrence and the transfer of tumor, can ease pain, toxic and side effects is little, can prolong cancer patient's life cycle.
The Chinese medicine of above-mentioned open source literature report can be treated from the angle of the traditional Chinese medical science, is suppressing aspect the tumor certain effect is arranged.But the prescription that has is complicated, have only in experimental stage, await clinical verification.
Summary of the invention
For reaching this purpose, the inventor studies the strong medical theory of tradition, on the basis of Zhuang nationality in Guangxi folk medicine, is replenished and puts in order, excavates and develop a kind of adjusting viscera function imbalance, suppresses strong medicine preparation of tumor and preparation method thereof.
Guangxi is the area that Zhuang is lived in concentrated communities, and Zhuang ancients have accumulated the experience of the local medical herbs resource treatment of abundant utilization tumor in secular life production practices and the process with the disease struggle.In recent years, under the joint efforts of various circles of society, strong medical research has been obtained the significance progress.The research of strong medical treatment tumor also makes encouraging progress.Unique prescription that multiple strong medicament extract and strong medicine are formed, compound recipe patent medicine are obtained good effect in the clinical and experimentation of anti-curing oncoma.The inventor being through understanding in depth and studying for many years, also sums up a kind ofly can suppress Chinese medicine preparation of tumor and preparation method thereof.
Antineoplastic of the present invention is strengthened the effective ingredient of medicine (Chinese medicine preparation) and is made by following Chinese herb raw materials by weight:
Herba Scutellariae Barbatae 18-30 part; Herba Hedyotidis Diffusae 18-30 part; Radix berchemiae lineatae 10-20 part; Radix Astragali 8-18 part;
Radix Scrophulariae 8-18 part; Radix Bupleuri 5-12 part; Radix Glycyrrhizae 1-6 part.
According to Chinese medicine document record, more than the source of said each composition of Chinese medicine be:
Herba Scutellariae Barbatae: the dry herb for labiate Herba Scutellariae Barbatae Scutellaria barbata D.Don meets " pertinent regulations under a 77-78 page or leaf of Chinese pharmacopoeia version in 2005 the Herba Scutellariae Barbatae item.Clean oven dry, cutting before feeding intake.
Herba Hedyotidis Diffusae: the herb for Rubiaceae cerastium plant Herba Hedyotidis Diffusae Oldenlandia diffusa (Willd.) Roxb. meets " pertinent regulations under 22 pages of Herba Hedyotidis Diffusae items of Chinese pharmacopoeia appendix of version in 2005.Clean oven dry, cutting before feeding intake.
Radix berchemiae lineatae: the dry root for Rhamnaceae plant Ramulus et Folium Berchemiae Lineatae Berchemia Lineata (L.) DC meets pertinent regulations under " Guangxi Chinese crude drug standard (1990) " 179 pages of Radix berchemiae lineatae items.Clean before feeding intake, cut into slices, dry.
The Radix Astragali: the dry root for leguminous plant Radix Astagali Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao or Radix Astragali Astragalus membranaceus (Fisch.) Bge. meets " pertinent regulations under a 212-213 page or leaf of Chinese pharmacopoeia version in 2005 Radix Astragali item.Clean before feeding intake, cut into slices, dry.
Radix Scrophulariae: the dry root for goatweed Radix Scrophulariae Scrophularia ningpoensis Hemsl. meets " pertinent regulations under a 76-77 page or leaf of Chinese pharmacopoeia version in 2005 the Radix Scrophulariae item.Clean before feeding intake.
Radix Bupleuri: the dry root for umbelliferae bupleurum Bupleurum chinense DC. or Radix Bupeuri Scorzonerfolii. Bupleurum scorzonerifolium Willd. meets the " pertinent regulations under 198 pages of Radix Bupleuri items of Chinese pharmacopoeia version in 2005.Clean oven dry, cutting before feeding intake.
Radix Glycyrrhizae: the dry root and rhizome for glycyrrhizic legume Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. Glycyrrhiza inflate Bat. or Glycyrrhiza glabra L. Glycyrrhiza glabra L. meets " pertinent regulations under a 59-60 page or leaf of Chinese pharmacopoeia version in 2005 the Radix Glycyrrhizae item.Clean oven dry, cutting before feeding intake.
The side of the strong medicine of the present invention separates:
The proved recipe of the strong medicine preparation of the present invention mainly is made up of medicines such as Radix berchemiae lineatae, Herba Hedyotidis Diffusae, Herba Scutellariae Barbataes, has the effect that dissipating blood stasis is swollen, heat clearing away is malicious, remove noxious dampness, lead to accent three road two-way.Be mainly used in the blood stasis poison and contain, gas the moon is the auxiliary treatment of the malignant tumor such as hepatocarcinoma of void.Radix berchemiae lineatae is lightly seasoned, puckery in the side, and property is flat; Transfer fiery road, dissipating blood stasis is swollen, the heat clearing away poison, and the poison that dispels the wind removes noxious dampness.Be used for tumor, farming sting (sore swollen toxin), digestive tract hemorrhage, trunk Yin (stomachache), energy freshwater mussel (jaundice), woods must sting phase diseases such as (traumatic injury).The Herba Hedyotidis Diffusae bitter in the mouth is sweet, and is cold in nature, and promoting blood circulation and stopping pain, is removed noxious dampness, waterway at the heat clearing away poison, be used for farming sting (sore swollen toxin), tumor, can freshwater mussel (jaundice), etc., modern pharmacological research proves that it has good antitumor, anti-inflammation, hepatic cholagogic effect.The Herba Scutellariae Barbatae flavor is peppery, bitter, cold in nature, transfers fiery road, and the heat clearing away poison removes noxious dampness; Be used for tumor, sting (furuncle), can freshwater mussel (jaundice), woods must sting phase (traumatic injury) etc.Three medicines share, the dissipating blood stasis detoxifcation, and logical accent road is principal agent, female medicine altogether.Radix Astragali bitter in the mouth is sweet, and is hot in nature; Benefiting QI for strengthening the superficies, inducing diuresis to remove edema, expelling pus and toxin by strengthening QI, expelling pus and promoting granulation; It is weak to be used for the deficiency of vital energy, anorexia and loose stool, and the exterior deficiency spontaneous perspiration, deficiency of vital energy edema, carbuncle is difficult bursts, and bursts for a long time and does not hold back, and blood deficiency dull yellowish colored skin etc. are public medicine in the side.The Radix Scrophulariae flavor is sweet, bitter, salty, is slightly cold; The removing heat from blood YIN nourishing, eliminating fire and detoxication; Be used for consumption of YIN caused by febrile disease, the crimson tongue excessive thirst, maculae caused by violent heat pathogen, constipation is hindered in Tianjin, carbuncle sore tumefacting virus; The Radix Bupleuri bitter cold; The heat clearing away poison removes noxious dampness, the poison that dispels the wind, and the pain in chest and hypochondrium of relieving stagnation of QI and the depress liver helps controlling of qi depression to blood stasis; Three medicines share, can strengthen principal agent, female medicine the heat clearing away poison, remove noxious dampness, swollen, the logical effect of transferring three road two-way of dissipating blood stasis, and take into account the void of gas the moon, be the group's medicine in the side altogether.Radix Glycyrrhizae is sweet flat, and coordinating the actions of various ingredients in a prescription and separate hundred poison is the band medicine.All medicines match, and bring out the best in each other, and play altogether that dissipating blood stasis is swollen, heat clearing away is malicious, remove the merit of noxious dampness, reach regulating QI, detoxifcation, tonify deficiency and logical effect of transferring three road two-way.Contain for the blood stasis poison, gas the moon is the auxiliary treatment of the malignant tumor such as hepatocarcinoma of void, has clinical effectiveness preferably.
More than all medicines share, reinforcement and elimination in combination, medicinal powder for relieving pain knot, giving consideration to both the incidental and fundamental is played nourishing YIN and benefiting blood, Yiqi and vein recovery, promoting blood circulation and stopping pain and adjusting human body function of immune system altogether, to the opposing tumor, keep the toxin expelling pipeline unimpeded, the growth of anticancer all has effect.
The strong medicine preparation of antineoplastic of the present invention can add filler or the solution that meets medical standard, make medically solid preparation or liquid preparation, for example tablet, capsule, granule, pill, oral liquid or syrup etc., below introduce the preparation method of several dosage forms respectively:
Described capsule preparation method thereof is as follows:
The ethanol extraction three times that above-described Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h filters merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into relative density is that 1.12-1.15 (60 ℃) and above-mentioned alcohol extraction concentrated solution merge, continue to be concentrated into the thick paste that relative density is 1.25-1.30 (60 ℃), drying under reduced pressure is ground into fine powder, adds fluidizer, filler, lubricants etc. are an amount of, mixing, dry-pressing is granulated, and is encapsulated, promptly.
The preparation method of described tablet is as follows:
The ethanol extraction three times that above-described Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h, filter, merging filtrate, placement is spent the night, and reclaims ethanol and is concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), regulates to contain the alcohol amount to 70%, and cold preservation is spent the night, reclaiming ethanol and being concentrated into relative density is 1.12-1.15 (60 ℃), merge with above-mentioned alcohol extraction concentrated solution, continue to be concentrated into the thick paste that relative density is 1.25-1.30 (60 ℃), drying under reduced pressure, be ground into fine powder, add disintegrating agent, fluidizer, filler, lubricant is an amount of, and mixing is granulated, tabletting, promptly get plain sheet, perhaps add sugar-coat, make coated tablet.
The preparation method of described granule is as follows:
The ethanol extraction three times that above-described Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h, filter, merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct each 0.5 hour three times, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), regulates to contain the alcohol amount to 70%, cold preservation is spent the night, reclaiming ethanol and being concentrated into relative density is that 1.12-1.15 (60 ℃) and above-mentioned alcohol extraction concentrated solution merge, and continues to be concentrated into the thick paste that relative density is 1.25-1.30 (60 ℃), drying under reduced pressure, be ground into fine powder, add an amount of sucrose or fluidizer again, mixing, granulate, promptly.
The preparation method of described pill is as follows:
The ethanol extraction three times that above-described Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h filters merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into relative density is that 1.12-1.15 (60 ℃) and above-mentioned alcohol extraction concentrated solution merge, continue to be concentrated into the thick paste that relative density is 1.25-1.30 (60 ℃), drying under reduced pressure is ground into fine powder, adds an amount of wetting agent again, binding agent, absorbent or diluent, mixing, the system gob, the pill bar is cut apart and round, make honeyed pill, paste pill or micropill, drying, packing, promptly.
The preparation method of described oral liquid is as follows:
The ethanol extraction three times that above-described Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h filters merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into relative density is that 1.12-1.15 (60 ℃) and above-mentioned alcohol extraction concentrated solution merge, add an amount of correctives and water again, packing, high temperature sterilize, promptly.
The preparation method of described syrup is as follows:
The ethanol extraction three times that above-described Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h filters merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into relative density is that 1.12-1.15 (60 ℃) and above-mentioned alcohol extraction concentrated solution merge, add an amount of sucrose and water again, packing, high temperature sterilize, promptly.
Above-described disintegrating agent, fluidizer, filler, lubricant can be selected starch, lactose, carboxymethyl cellulose, Polyethylene Glycol, calcium stearate, calcium carbonate, Pulvis Talci and dextrin etc. pharmaceutically commonly used for use.
Above-described correctives can comprise: saccharin sodium, Sorbitol, xylitol and aspartame etc.
Outstanding substantive distinguishing features of the present invention and obvious improvement:
The present invention adds that by Radix berchemiae lineatae, Herba Hedyotidis Diffusae and the Herba Scutellariae Barbatae of main product in Guangxi the Radix Astragali, Radix Scrophulariae, Radix Bupleuri, Radix Glycyrrhizae etc. form, and prescription derives from Zhuang clinical experience side among the people.According to the characteristics of its medication, by the further investigation of aspects such as galenic pharmacy, pharmacodynamics, toxicology and quality control, various preparation safeties, effective, steady quality, controlled.
Various liquid preparations that the present invention makes or solid preparation can be used for the auxiliary treatment of malignant tumor such as hepatocarcinoma, pulmonary carcinoma.Now just Chinese medicine antitumor action mechanism of the present invention is summarized as follows:
The Chinese herbal treatment malignant tumor, be that wholistic therapy combines with determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs, just whole and part combines, has both seen the defensive ability/resistance ability of body to pathogenic factor, see the influence of local patholoic change again to body, on differential diagnosis of diseases and dialectical basis, by the composition of prescription of some specific drugs with strong points, enter body rightly, regulate the balance of QI and blood, negative and positive, body fluid.Drug molecule enters whole body through physical reactions and chemical reaction, and killing cancer cell is enclosed in the surface that is adsorbed on cell that has, corrosion, and this can be called " battle of encirclement "; The medicament composing prescription that has by different effects, is cut apart cancerous cell, defeats in detail again, and this can be called " fighting war "; The effect of passing through medicine itself that has, attached with synergistic medicine, can tumoricidal blood circulation, remove and change the partial blood fortune of body, the propagation of control cancer cell, this can be called " instigating rebellion within enemy camp war "; Have in conjunction with body condition, by medicine transfer, the function of enhancing, human body immunity improving cell removes to engulf cancerous cell, this can be called " immunity war "; The nature and flavor that pass through medicine that have add and use local application, go tumoricidal structure and generation with temperature, and this can be called " jamming war "; The medicine that has can be regulated QI and blood, can make QI-blood circulation normal, isolates cancerous cell, makes it to excrete, and this can be called " expulsion war "; What have can influence the cancerous cell endoenzyme, makes it become " end fat ", makes cancerous cell chromosome, gene necrosis, and this can be called " sabotage operations "; Can help immunocyte chromosome, the gene that have restore, and this can be called " drawing war over to one's side "; What have can make QI and blood energy mean allocation. hungry cancerous cell, make it to revert to normal cell, and this can be called " balance war ".
The inventor thinks: traditional immune notion is meant the ability of body opposing pathogenic microorganism, i.e. infection immunity.Along with science and technology development, the notion of immunity has substantially exceeded the scope of infection immunity.Therefore, the notion of modern immunity is: immunity is a kind of physiological function of body, rely on this function identification " oneself " and " non-own " composition, thereby destruction and repulsion enter the intravital antigenic substance of machine (as pathogen, allosome tissue etc.), or body itself old dead and damaged cell, the tumor cell etc. that are produced, with stablizing of environment in keeping.
So, strong medicine preparation of the present invention has been followed motherland's medical science organic conception and determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs principle, the blood circulation promoting and blood stasis dispelling of employing Radix berchemiae lineatae, Herba Hedyotidis Diffusae and Herba Scutellariae Barbatae, hard masses softening and resolving, heat-clearing and toxic substances removing, the body resistance strengthening and constitution consolidating etc. have " operation " means of traditional Zhuang characteristic medicine, accomplish " medication is as using military forces ", ability " To know one's own strength and the enemy's is the sure way to victory ", in the assault of fortified positions of going on an expedition cancer, handy, set up outstanding service, make traditional medicine demonstrate immeasurable huge effect.
The present invention carries out labor to each medicine:
1. Herba Scutellariae Barbatae: be one of research focus of antitumor Chinese medicine both at home and abroad in recent years, modern study and clinical proof, Herba Scutellariae Barbatae has good antineoplastic activity, cure mainly gynecological tumors such as digestive tract tumor, pulmonary carcinoma and cervical cancer such as primary hepatocarcinoma, and unite the compound treatment kinds of tumors with other Chinese medicines.Herba Scutellariae Barbatae mainly contains flavone compound, diterpene and diterpene ginkgolide, alkaloid, polysaccharide composition, micro constitutent etc.Zhang Chunling etc. studies show that: the water solubility extract of Herba Scutellariae Barbatae can resist NNK, and (NNK is the very strong nitrosamines material of a kind of carcinogenecity, for bringing out one of principal element of human tumor) mutagenicity, test strain back mutation number is reduced, and raise along with respectively trying drug concentrations, its antagonism strengthens, and shows and contains the mutation thing in the Herba Scutellariae Barbatae.The Herba Scutellariae Barbatae water extract has very strong antimutagenic effect, can significantly suppress the sudden change bacterium colony element of mutation such as flavacin B2, and the complex that generates with DNA is reduced; Also can obviously suppress the TA98 that benzopyrene brings out and the effect of TA100 back mutation.Non-program DNA synthetic test (UDS test) by human peripheral lymphocyte such as Han Fabin finds that concentration is the mutagenic action that the Herba Scutellariae Barbatae extract of 125g/L has certain anti-cigarette coke tar, can protect lymphocyte.The Herba Scutellariae Barbatae polysaccharide promotes inductive mouse boosting cell lymphocyte transformation external, obviously improve the percentage rate of esterase positive cell in the mice peripheral blood lymphocyte, promote inductive delayed allergy, illustrate that this medicine has facilitation to the cellular immunization of body.According to above-mentioned experiment report, we intend adopting the technology of boiling with the common decocting of other medical materials, and extracts active ingredients such as the flavone in the Herba Scutellariae Barbatae, polysaccharide are come out.
2. Herba Hedyotidis Diffusae: domestic chemical constitution study to Herba Hedyotidis Diffusae is since the sixties, confirming that its main component has: 6-0-is right-the coumaric acyl scandoside methyl ester, 6-0-is to methoxyl group cinnamyl scandoside methyl ester, 6-0-Resina Ferulae acyl scandoside methyl ester, iridoid constituents such as Asperula orientalis glucosides, triterpenes components: oleanolic acid, maloic acid, the sub-ketone of Chinese lime, isoarborinol etc., and sterols composition: B-sitosterol, B-sitosterol-D-glucoside, anthraquinone component: 2-methyl-3-hydroxyanthraquinone, 2-methyl-3-methoxyl group anthraquinone, and alkaloid, cardiac glycoside etc.In recent years studies show that Herba Hedyotidis Diffusae has enhancing non-specific immunity effect and antioxidation, be used for the treatment of various cancers and inflammation clinically, but the antineoplastic active component fails to determine always.Zhao Haoru has studied the influence of the different extraction processes of Herba Hedyotidis Diffusae to anti-tumor activity, the direct water extract of experimental result prompting Herba Hedyotidis Diffusae in vivo to the S-180 tumor strain do not have tangible anti-tumor activity, and water miscible polysaccharide and lipophilic extract have significant inhibitory effect to the S-180 tumor cell, fat-soluble position and the polysaccharide of prompting Herba Hedyotidis Diffusae have good antitumor application prospect, extract more effective than simple water extraction respectively.Tan Ninghua etc. in the state-run institute of oncology of the U.S. to the ethanol extraction of Herba Hedyotidis Diffusae with different solvent extraction, extract has been carried out the cytotoxic activity screening of tumor cell line, find that there is certain cytotoxic activity at CH3OH:H20 (9:1) position, particularly has the moderate strength activity to breast carcinoma cell strain.With reference to pertinent literature report, we determine that Herba Hedyotidis Diffusae and Radix berchemiae lineatae use ethanol extraction jointly.
3. Radix berchemiae lineatae: be Guangxi medical material among the people, record in nineteen ninety version " Guangxi Chinese crude drug standard ", bitter in the mouth, little puckery, property is put down, return the liver lung meridian, have subduing swelling and detoxicating, hemostatic analgesia, expelling wind and removing dampness effect, the diseases such as pulmonary tuberculosis, haematemesis, icterohepatitis, stomachache, headache, traumatic injury, furuncle swelling toxin that are used for the treatment of among the people.Relevant Radix berchemiae lineatae medical material The Chemical Constituents report is less, " Guangxi Chinese crude drug standard " rutin (Rutin) is rutin again, be the 3-0-rutinoside of Quercetin (quercetin), rutin and derivant tool thereof be pharmacologically active widely, but rutin and its aglycon quercetin all are insoluble in water, we by trial test, determine that Radix berchemiae lineatae extracts with ethanol in conjunction with the character of other composition in this product prescription.
4. the Radix Astragali: main component is saponin, flavone, polysaccharide, aminoacid and trace element etc.Effect with antiinflammatory, blood pressure lowering, analgesia, defying age, antiviral, antitumor, enhancing immunity.Astragaloside (AsI) is the main component of more than ten kind of Saponin in the Radix Astragali, is the index components of formulation of astragalus root quality arbitration, and structure is a triterpenoid saponin.Its pharmacological evaluation shows: ASI has antiinflammatory, blood pressure lowering, analgesia, sedation.The content of CAMP and Liver Regeneration DNA in the blood plasma can be increased, mouse thymus weight can be increased, promptly influential to immune organ.ASI can obviously improve impaired leukocyte deformability and the improvement of leukocyte deformability may be one of mechanism of action of control cardiovascular and cerebrovascular disease.ASI has effect to pancreas, finds that ASI can promote secretion of insulin.ASI can remove free radical, and cerebral tissue is had the certain protection effect.(Astra-galus polysaccharids is a most important natural constituent in the Radix Astragali APS) to astragalus polysaccharides, has the immunologic function of promotion, improves macrophage activity, suppresses effects such as EAS, two-ways regulation blood glucose.The biological activity of astragalus polysaccharides is relevant with molecular weight, dissolubility, viscosity, primary structure and higher structure.According to Milkvetch Root composition and pharmacological action, and in conjunction with the characteristics of capsule, the extracting method that we have adopted decocting to boil to the Radix Astragali.
5. Radix Scrophulariae: the beginning is stated from Shennong's Herbal, cold nature, and sweet in the mouth, hardship, salty have YIN nourishing, and pathogenic fire reducing promotes the production of body fluid, removing heat from blood, effects such as detoxifcation.Its chemical constituent is the plain class (angole glycoside C etc.) of iridoids (Harpagide, harpagoside etc.), phenylpropyl alcohol, still contains plant sterol in addition, organic acid, flavonoid, triterpene saponin, volatile oil, saccharide, the monoterpene of alkaloid and trace and two terpene components etc.Wherein the plain class of iridoids and phenylpropyl alcohol is the main component of Radix Scrophulariae, and the pharmacologically active of these compositions is consistent with clinical effect, can be used as the material base of the pharmacological action of Radix Scrophulariae.Discovery Radix Scrophulariaes such as Gong Weigui alcohol extractum aqueous solution can significantly increase the extrasomatic rabbit heart coronary flow, simultaneously heart rate, heart contractility is had slight inhibition; Radix Scrophulariae can obviously increase mouse cardiac muscle nutrient flow amount, and the arteria coronaria contraction due to the mice pituitrin is had obvious antagonism.Simultaneously, pharmacological research shows that Radix Scrophulariae infusion, alcohol-water immersion and decoct have significant hypotensive effect to multiple animals such as anesthesia Canis familiaris L., cat, rabbits.Healthy dog and the oral decoct 2g/kg of kidney type hypertension Canis familiaris L., 2 times/d, all show hypotensive effect, and render a service more remarkable than healthy dog to the blood pressure lowering of kidney type hypertension Canis familiaris L., delaying appears in the hypotensive effect of smaller dose Radix Scrophulariae, and Lee cures bright grade and discovers the plain glycosides XS-8 of phenylpropyl alcohol, XS-10 and iridoid glycoside XS-6, X-7 has antiplatelet aggregative activity under 0.5mmol/L, but the plain glycosides effect of phenylpropyl alcohol is stronger.Huang Caiguo etc. discover that further the plain glycosides XS-8 of phenylpropyl alcohol all has the reduction effect to TXB2 in the blood plasma and 6-keto-PGF10, but more obvious to the reduction effect of TXB2, may all be the mechanism of its antiplatelet aggregation.Xie Lihua etc. discover the immunologic function recovery that the harpagoside subcutaneous injection can make deficiency of YIN mice suppress; Harpagide and harpagoside all can promote the external spleen lymphocyte proliferation of deficiency of YIN mice.
6. Radix Bupleuri: cold nature, bitter in the mouth, little suffering, merit can be gently clear, rise loose, catharsis, can expelling pathogenic factors from the exterior bring down a fever, dispersing the stagnated live-QI to relieve the stagnation of QI, but elevate a turnable ladder yang-energy again can be controlled menoxenia.Commonly used clinically its evacuated the heresy of few positive half exterior and half interior.The disease of treatment alternate attack of chill and fever, collapse of QI.Main component is a saikoside in the Radix Bupleuri root, and next contains plant sterol, adonite, and a small amount of volatile oil, polysaccharide; Aerial parts mainly contains flavonoid, small amounts of soap glycoside, lignanoids, Coumarins composition.Discover that saikoside has effects such as antiinflammatory, antiviral, antitumor, endocrine regulation and immune system, the Mus foot edema that on Carrageenan, dextran, 5-hydroxy tryptamine, Oleum Tiglii or acetic acid cause has the obvious suppression effect.Saikoside Sb1, Sb2, Sb3, Sb4 that saikoside a, d and secondary generate have very strong inhibitory action to the Na+2K+2ATP enzyme, can cause the variation of energy and water-electrolyte metabolism, thereby play antiviral effect.The saikoside d antiinflammatory action is the strongest, and the mechanism of its effect is directly to stimulate adrenal cortex, and glucocorticoid secretion is increased.In addition, the Radix Bupleuri polysaccharide can promote that hematopoietic stem cell breaks up to lymphocyte, strengthens the macrophage phagocytic ability, inducement interferon, activate immunity function.Because the polarity of saikoside is bigger, water extraction method or the Different concentrations of alcohol solution of adopting extract more in the bibliographical information, Huo Wuzheng etc. study Radix Bupleuri extracts active ingredients condition, use respectively: water extraction 2h, 60% ethanol extraction 2h and three kinds of same methods of cloth of ethanol extraction 1h of first water extraction 1h reuse 60%, the result is that water extraction content is 0.29mg/ml, ethanol extraction method is 0.12mg/ml, and the water containing ethanol extraction method is 0.17mg/ml.As seen, saikoside a content is apparently higher than all the other two methods in the water extraction method, and water extraction method is beneficial to and reduces production costs, therefore, and the extracting method that adopts decocting to boil to Radix Bupleuri.
7. Radix Glycyrrhizae: main effect is and middle emergency, lung moistening, detoxifcation, coordinating the actions of various ingredients in a prescription etc., application is all arranged in most prescriptions, verified, Radix Glycyrrhizae still has antiinflammatory, antiallergic action, antiulcer, prevents and treats viral hepatitis, effects such as anticancer, enhance immunity sexual function and anti-AIDS except that having effect such as convulsion, analgesia, antitussive.Its chemical constituent is glycyrrhizin (mainly be potassium, the calcium salt of glycyrrhizic acid, be the sweet ingredient of Radix Glycyrrhizae), multiple flavone compound, multiple triterpenoid compound and coumarin, aminoacid, alkaloid, estrogen and organic acid etc.Wherein glycyrrhizin is its main component; experimentation shows; glycyrrhizin (GL) all has the obvious suppression effect to the generation that aflatoxin B1 (AFB1) and diethylnitrosamine (DEN) cause the rats'liver precancerous lesion; and reparation has significant protective effect .GL to make the DNA repair function near normal level to GL to DNA damage in the pathological changes generating process before suppressing DEN to cause hepatocarcinoma; thereby reduced the carcinogenecity of DEN, the positive liver cell proliferation kitchen range of pathological changes γ glutamate-transpeptidase reduced before GL also can make hepatocarcinoma simultaneously.In addition, the enoxolone list glucuronic acid glycoside (MGGR) that obtains through enzymatic hydrolysis of glycyrrhizin mouse skin cancer, pulmonary carcinoma that multiple reason is brought out has inhibitory action.Take the drinking water that adds glycyrrhizin for a long time and can suppress 7, the mice canceroderm that 12-dimethyl-benzanthracene (DMBA) causes.Be present in the Radix Glycyrrhizae because glycyrrhizic acid generally is the form with potassium salt or calcium salt, its salt is soluble in water, so the extracting method that Radix Glycyrrhizae employing decocting is boiled.
The specific embodiment
Following embodiment is the prescription and the preparation method of the strong medicine preparation of antineoplastic of the present invention, but the invention is not restricted to the content of embodiment:
Embodiment one
Get following parts by weight of traditional Chinese medicines raw material and adjuvant:
Radix berchemiae lineatae 200g, Herba Hedyotidis Diffusae 500g, Herba Scutellariae Barbatae 500g, Radix Astragali 200g, Radix Scrophulariae 200g, Radix Bupleuri 150g, Radix Glycyrrhizae 50g, starch 100g, Polyethylene Glycol 100g, carboxymethyl cellulose 150 grams, Pulvis Talci 150g.
Earlier Radix berchemiae lineatae and Herba Hedyotidis Diffusae are added 8 times of amount 80% ethanol extractions three times, each 2h filters, merging filtrate, and placement is spent the night, and reclaiming ethanol and being concentrated into relative density is 1.12-1.15 (60 ℃).All the other five tastes such as Herba Scutellariae Barbatae add 10 times of water gagings, decoct three times, each 0.5 hour, filter collecting decoction, being concentrated into relative density is 1.05 (60 ℃), adjusting contains the alcohol amount to 70%, and cold preservation is spent the night, and reclaiming ethanol and being concentrated into relative density is 1.12-1.15 (60 ℃), merge with above-mentioned alcohol extraction concentrated solution, continue to be concentrated into the thick paste that relative density is 1.25-1.30 (60 ℃), drying under reduced pressure is ground into fine powder, add starch, Polyethylene Glycol, carboxymethyl cellulose, mixing, granulate, tabletting is made 1000 tablets, promptly.
Embodiment two
Get following parts by weight of traditional Chinese medicines raw material and adjuvant:
Radix berchemiae lineatae 333g, Herba Hedyotidis Diffusae 500g, Herba Scutellariae Barbatae 500g, Radix Astragali 278g, Radix Scrophulariae 278g, Radix Bupleuri 167g, Radix Glycyrrhizae 83g, lactose 300g.
Earlier Radix berchemiae lineatae and Herba Hedyotidis Diffusae are added 8 times of amount 80% ethanol extractions three times, each 2h filters, merging filtrate, and placement is spent the night, and reclaiming ethanol and being concentrated into relative density is 1.12-1.15 (60 ℃).All the other five tastes such as Herba Scutellariae Barbatae add 10 times of water gagings, decoct each 0.5 hour three times, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), regulates to contain the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into relative density is that 1.12-1.15 (60 ℃) and above-mentioned alcohol extraction concentrated solution merge, continue to be concentrated into the thick paste that relative density is 1.25-1.30 (60 ℃), drying under reduced pressure is ground into fine powder, add lactose, mixing, dry-pressing is granulated, encapsulated, make 1000, promptly.
Embodiment three
Get following parts by weight of traditional Chinese medicines raw material and adjuvant:
Radix berchemiae lineatae 120g, Herba Hedyotidis Diffusae 400g, Herba Scutellariae Barbatae 350g, Radix Astragali 150g, Radix Scrophulariae 180g, Radix Bupleuri 120g, Radix Glycyrrhizae 40g, lactose 100g, xylitol 200 grams.
The ethanol extraction three times that above-described Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h, filter, merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct each 0.5 hour three times, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), regulates to contain the alcohol amount to 70%, cold preservation is spent the night, reclaiming ethanol and being concentrated into relative density is that 1.12-1.15 (60 ℃) and above-mentioned alcohol extraction concentrated solution merge, and continues to be concentrated into the thick paste that relative density is 1.25-1.30 (60 ℃), drying under reduced pressure, be ground into fine powder, add lactose and xylitol again, mixing, make granule, promptly.
Embodiment four
Get following parts by weight of traditional Chinese medicines raw material and adjuvant:
Radix berchemiae lineatae 250g, Herba Hedyotidis Diffusae 300g, Herba Scutellariae Barbatae 400g, Radix Astragali 300g, Radix Scrophulariae 250g, Radix Bupleuri 80g, Radix Glycyrrhizae 30g, starch 100g, sucrose 250 grams, Mel 150g.
Radix berchemiae lineatae and Herba Hedyotidis Diffusae are added the ethanol extraction three times of 8 times of amount volumetric concentration 70-90%, and each 2h filters, merging filtrate, placement is spent the night, and reclaims ethanol and is concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct each 0.5 hour three times, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), adjusting contains the alcohol amount to 70%, and cold preservation is spent the night, and reclaiming ethanol and being concentrated into relative density is 1.12-1.15 (60 ℃), merge with above-mentioned alcohol extraction concentrated solution, continue to be concentrated into the thick paste that relative density is 1.25-1.30 (60 ℃), drying under reduced pressure, be ground into fine powder, add starch again, sucrose, Mel, mixing, the system gob, the pill bar is cut apart and round, make 100 of honeyed pill, drying, packing, promptly.
Embodiment five
Get following parts by weight of traditional Chinese medicines raw material and adjuvant:
Radix berchemiae lineatae 300g, Herba Hedyotidis Diffusae 520g, Herba Scutellariae Barbatae 500g, Radix Astragali 200g, Radix Scrophulariae 200g, Radix Bupleuri 150g, Radix Glycyrrhizae 60g, Sorbitol 300g.
The ethanol extraction three times that Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h filters merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into relative density is that 1.12-1.15 (60 ℃) and above-mentioned alcohol extraction concentrated solution merge, add Sorbitol and 1000ml water again, packing, high temperature sterilize promptly gets oral liquid.
Embodiment six
Get following parts by weight of traditional Chinese medicines raw material and adjuvant:
Radix berchemiae lineatae 150g, Herba Hedyotidis Diffusae 320g, Herba Scutellariae Barbatae 300g, Radix Astragali 200g, Radix Scrophulariae 150g, Radix Bupleuri 180g, Radix Glycyrrhizae 60g, sucrose 500 grams, aspartame 100g.
The ethanol extraction three times that Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h, filter, merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into the dope that relative density is 1.12-1.15 (60 ℃), again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into relative density is 1.05 (60 ℃), adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into relative density is that 1.12-1.15 (60 ℃) and above-mentioned alcohol extraction concentrated solution merge, again with sucrose and aspartame and 500ml water, packing, high temperature sterilize promptly gets syrup.
The Pharmacodynamic test of active extract of medicine of the present invention (replacement of following usefulness " ZT " code name)
The medicine ZT of invention is made up of Radix berchemiae lineatae, Oldenlandia diffusa Roxb, Herba Scutellariae Barbatae, Radix Scrophulariae, the Radix Astragali, Radix Glycyrrhizae, Radix Bupleuri etc.The merit that has dispersing swelling and dissipating binds, gets rid of evils and set upright, its clinical effectiveness is remarkable.Radix berchemiae lineatae mildly bitter flavor in the side, property is flat, and the merit of detoxifcation, dissipating blood stasis for subsidence of swelling, expelling wind and removing dampness is arranged, and is used for diseases such as sore swollen toxin, digestive tract hemorrhage, traumatic injury; Radix Astragali sweet in the mouth, temperature has the merit of poison holding, evacuation of pus, expelling pus and promoting granulation, with external antitumor action is arranged all in vivo; Herba Scutellariae Barbatae acrid in the mouth, hardship, cold in nature, return lung, liver, kidney channel, effect with heat-clearing and toxic substances removing, dissipating blood stasis hemostasis, inducing diuresis to remove edema, be usually used in scabies toxic swelling, laryngopharynx swelling and pain, venom, fall diseases such as pouncing on the pain of injury, edema, jaundice, all medicines share, and playing altogether gets rid of evils sets upright, the merit of dispersing swelling and dissipating binds.Cure mainly and clinical indication according to its composition, function, this medicine has been carried out antitumor action, the pharmacodynamic study of aspects such as centralizing function, main result is as follows:
One, the eliminating evil effect of ZT
1, adopt mtt assay to measure the inhibitory action of ZT pastille serum, with crude drug 10g/kg dosage (be clinical plan consumption 15.6 times) gastric infusion, every day twice, continuous 5 days to hepatocarcinoma H22 cell proliferation.The result shows that 20%ZT pastille serum can obviously suppress the propagation of hepatocarcinoma H22 cell, compares p＜0.05 with the blank group.
2, adopting hepatocarcinoma H22 transplanted solid tumor mice is animal model, heavily is the antitumor action that evaluation index is observed ZT with tumor.With crude drug 20g, 10g, 5g/kg dosage (be respectively clinical plan consumption 31.3,15.6,7.8 times) gastric infusion, once a day, continuous 28 days.Experiment is carried out three batches altogether, and first result shows that ZT20g/kg, 10g/kg dosage group (be respectively clinical plan consumption 31.3,15.6 times) can obviously suppress tumor growth, with model group p＜0.05～0.01 relatively.Second and third batch result shows that ZT20g, 10g, 5g/kg dosage group all can obviously suppress tumor growth, compares p＜0.05～0.01 with model group.
Two, the centralizing function of ZT
1, the hepatocarcinoma H22 tumor-bearing mice that adopts intraperitoneal injection of cyclophosphamide to cause immunocompromised is a model, observes the influence of ZT to the mice serum hemolysin.With mice hemolysin content (HCIgM) is that index evaluation ZT is to Immune Effects.With crude drug 20g, 10g, 5g/kg dosage (be respectively clinical plan consumption 31.3,15.6,7.8 times) gastric infusion, once a day, continuous 28 days.The result shows ZT20g/kg dosage group (for 31.3 times of clinical plan consumption), obviously improves hemolysin content, with model group p＜0.05 relatively.
2, the hepatocarcinoma H22 tumor-bearing mice that adopts intraperitoneal injection of cyclophosphamide to cause immunocompromised is a model, observes the influence that ZT cleans up mice carbon.To clean up index K value and correction coefficient a value is that evaluation index is observed ZT to Immune Effects.With crude drug 20g, 10g, 5g/kg dosage (be respectively clinical plan consumption 31.3,15.6,7.8 times) gastric infusion, once a day, continuous 28 days.The result shows that 20g/kg, 10g/kg dosage group (be respectively clinical plan consumption 31.3,15.6 times) can obviously improve the mononuclear cell phagocytic function that cyclophosphamide causes the hepatocarcinoma H22 tumor-bearing mice of immunocompromised, with model group p＜0.01 relatively.
3, the hepatocarcinoma H22 tumor-bearing mice that adopts intraperitoneal injection of cyclophosphamide to cause immunocompromised is a model, observes ZT to the active influence of NK cells in mice.With NK cell killing rate is that evaluation index is observed ZT to Immune Effects.With crude drug 20g, 10g, 5g/kg dosage (be respectively clinical plan consumption 31.3,15.6,7.8 times) gastric infusion, once a day, continuous 28 days.The result shows that 20g/kg, 10g/kg, 5g/kg dosage group (be respectively clinical plan consumption 31.3,15.6,7.8 times doubly) all can obviously improve the NK cytoactive that cyclophosphamide causes the hepatocarcinoma H22 tumor-bearing mice of immunocompromised, with model group p＜0.01 relatively.
3, the hepatocarcinoma H22 tumor-bearing mice that adopts the lumbar injection dexamethasone to cause immunocompromised is a model, observes the centralizing function of ZT with the content evaluation index of mouse cell factor IL-2, TNF-β.With crude drug 20g, 10g, 5g/kg dosage (be respectively clinical plan consumption 31.3,15.6,7.8 times) gastric infusion, once a day, continuous 28 days.The result shows that ZT does not have obvious effect to cytokine IL-2, the TNF-β that dexamethasone causes the hepatocarcinoma H22 tumor-bearing mice of immunocompromised, compares p＞0.05 with model group.
Three, conclusion
This experimental result shows that ZT has certain antitumor action, adopt mtt assay to measure the inhibitory action of ZT pastille serum to hepatocarcinoma H22 cell proliferation, employing to the subcutaneous H22 solid tumor mouse model that causes of mice, is studied ZT antitumaous effect in vivo with tumor cell transplantation.The result shows that ZT pastille serum has obvious inhibitory action to the propagation of hepatocarcinoma H22 cell; Compare with the blank group, strong medicine ZT has obvious inhibitory action to the growth of mice H22 solid tumor, for its clinical practice provides certain theoretical foundation.
This experimental result shows that ZT has centralizing function, ZT can improve the carbon of immunosuppressant tumor-bearing mice and clean up index, can promote the non-specific immunity of MPS, ZT can start the antigen presentation of MPS to the stimulation of MPS, antigen is discerned by lymphocyte easily and utilized induce immune response.ZT has tangible rising effect to the hepatocarcinoma H22 tumor-bearing mice serum hemolysin content of immunocompromised due to the intraperitoneal injection of cyclophosphamide; Can obviously improve the antibody IgM level, the IgM that the antagonism cyclophosphamide causes reduces, and ZT mainly realizes by regulating antibody horizontal immune effect.ZT can obviously improve the mononuclear phagocyte function of the hepatocarcinoma H22 tumor-bearing mice of immunocompromised due to the intraperitoneal injection of cyclophosphamide, can obviously improve the hepatocarcinoma H22 tumor-bearing mice NK cytoactive of immunocompromised due to the intraperitoneal injection of cyclophosphamide; ZT does not have obvious effect to cytokine IL-2, the TNF-β that dexamethasone causes the hepatocarcinoma H22 tumor-bearing mice of immunocompromised.
Above result shows that ZT has obvious antitumor action and can improve immunologic function to immunocompromised hepatocarcinoma H22 tumor-bearing mice hepatocarcinoma H22.
The treatment of cancer example:
Liu * *, the man, nineteen forty-two gives birth to, Guilin people from city finds abdominal pain, belly distending pain all day, the soreness of waist checks that liver has lump, and biopsy is a hepatocarcinoma, do hepatectomy in certain tumour hospital at the beginning of 2009, cut 1/4 liver, after 5 months, find abdominal pain once more, hospital required patient to do chemotherapy at that time, patient disagrees with, after change the granule of taking medicine embodiment three of the present invention, twice of every day, each 10 grams, take belly distending pain after 20 days, soreness of waist symptom takes a turn for the better, take one month after, belly distending pain improving situation was survived 1 year.
Wei * *, life in 1967, people from Chongzuo City, Guangxi, on June 10th, 2009 was suffered from pulmonary carcinoma, right lung pain, dyspnea at examination in hospital, be not in hospital owing to lack of capital, take the capsule of the embodiment of the invention two, every day 3-4 time, totally 15 grams, take the aftersensation in January and get that sb.'s illness took a favorable turn, breathe smooth and easy, present right lung pain, dyspneic symptom takes a turn for the better.
The Qin * *, woman, life in 1962, the teacher of Nanning technical school, in December, 2010 is suffered from hepatocarcinoma, at Nanning hospital hospitalization, checks and shifts, the abdominal part distending pain is not operated on and is performed an operation, and adopts conservative treatment, family members require to take the Chinese medicinal tablet of the embodiment of the invention one, every day 3-4 time, totally 20 grams, take the aftersensation in January and get that sb.'s illness took a favorable turn, abdominal part distending pain symptom takes a turn for the better, and can arrive outdoor activity, continued to take 2 months, but take a walk to the outside every day, work can do housework.
Claims (8)
1. an antineoplastic is strengthened medicine, and it is characterized in that: its effective ingredient is made by following Chinese herb raw materials by weight:
Radix berchemiae lineatae 10-20 part; Herba Scutellariae Barbatae 18-30 part; Herba Hedyotidis Diffusae 18-30 part; Radix Astragali 8-18 part;
Radix Scrophulariae 8-18 part; Radix Bupleuri 5-12 part; Radix Glycyrrhizae 1-6 part.
2. antineoplastic according to claim 1 is strengthened medicine, it is characterized in that: add the filler or the solution that meet medical standard, make medically solid preparation or liquid preparation; Described solid preparation is capsule, tablet, pill or granule; Described liquid preparation is oral liquid or syrup.
3. according to claim 1 and the strong medicine of 2 described antineoplastic, it is characterized in that: described capsule preparation method thereof is as follows:
Radix berchemiae lineatae and Herba Hedyotidis Diffusae are added the ethanol extraction three times of 8 times of amount volumetric concentration 70-90%, and each 2h filters, merging filtrate, and placement is spent the night, and reclaims ethanol and be concentrated into 60 ℃ of relative densities the dope that is 1.12-1.15, again with all the other five tastes; Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into 60 ℃ of relative densities is 1.05, adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into 60 ℃ of relative densities is that 1.12-1.15 and above-mentioned alcohol extraction concentrated solution merge, continue to be concentrated into 60 ℃ of relative densities the thick paste that is 1.25-1.30, drying under reduced pressure is ground into fine powder, and it is an amount of to add fluidizer, filler, lubricant, mixing, dry-pressing is granulated, and is encapsulated, promptly.
4. according to claim 1 and the strong medicine of 2 described antineoplastic, it is characterized in that: the preparation method of described tablet is as follows:
Radix berchemiae lineatae and Herba Hedyotidis Diffusae are added the ethanol extraction three times of 8 times of amount volumetric concentration 70-90%, and each 2h filters, merging filtrate, placement is spent the night, and reclaims ethanol and be concentrated into 60 ℃ of relative densities the dope that is 1.12-1.15, again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into 60 ℃ of relative densities is 1.05, regulates to contain the alcohol amount to 70%, and cold preservation is spent the night, reclaiming ethanol and being concentrated into 60 ℃ of relative densities is 1.12-1.15, merge with above-mentioned alcohol extraction concentrated solution, continue to be concentrated into 60 ℃ of relative densities the thick paste that is 1.25-1.30, drying under reduced pressure, be ground into fine powder, add disintegrating agent, fluidizer, filler, lubricant is an amount of, and mixing is granulated, tabletting, promptly get plain sheet, perhaps add sugar-coat, make coated tablet.
5. according to claim 1 and the strong medicine of 2 described antineoplastic, it is characterized in that: the preparation method of described granule is as follows:
The ethanol extraction three times that Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h, filter, merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into 60 ℃ of relative densities the dope that is 1.12-1.15, again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct each 0.5 hour three times, filter, collecting decoction, being concentrated into 60 ℃ of relative densities is 1.05, regulates to contain the alcohol amount to 70%, cold preservation is spent the night, reclaiming ethanol and being concentrated into 60 ℃ of relative densities is that 1.12-1.15 and above-mentioned alcohol extraction concentrated solution merge, and continues to be concentrated into 60 ℃ of relative densities the thick paste that is 1.25-1.30, drying under reduced pressure, be ground into fine powder, add an amount of sucrose or fluidizer again, mixing, granulate, promptly.
6. according to claim 1 and the strong medicine of 2 described antineoplastic, it is characterized in that: the preparation method of described pill is as follows:
The ethanol extraction three times that Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h filters merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into 60 ℃ of relative densities the dope that is 1.12-1.15, again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into 60 ℃ of relative densities is 1.05, adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into 60 ℃ of relative densities is that 1.12-1.15 and above-mentioned alcohol extraction concentrated solution merge, continue to be concentrated into 60 ℃ of relative densities the thick paste that is 1.25-1.30, drying under reduced pressure is ground into fine powder, adds an amount of wetting agent again, binding agent, absorbent or diluent, mixing, the system gob, the pill bar is cut apart and round, make honeyed pill, paste pill or micropill, drying, packing, promptly.
7. according to claim 1 and the strong medicine of 2 described antineoplastic, it is characterized in that: the preparation method of described oral liquid is as follows:
The ethanol extraction three times that Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h filters merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into 60 ℃ of relative densities the dope that is 1.12-1.15, again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into phase is 1.05 to density for 60 ℃, adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into 60 ℃ of relative densities is that 1.12-1.15 and above-mentioned alcohol extraction concentrated solution merge, add an amount of correctives and water again, packing, high temperature sterilize, promptly.
8. according to claim 1 and the strong medicine of 2 described antineoplastic, it is characterized in that: the preparation method of described syrup is as follows:
The ethanol extraction three times that Radix berchemiae lineatae and Herba Hedyotidis Diffusae is added 8 times of amount volumetric concentration 70-90%, each 2h filters merging filtrate, placement is spent the night, reclaim ethanol and be concentrated into 60 ℃ of relative densities the dope that is 1.12-1.15, again with all the other five tastes: Herba Scutellariae Barbatae, the Radix Astragali, Radix Scrophulariae, Radix Bupleuri and Radix Glycyrrhizae add 10 times of water gagings, decoct three times, each 0.5 hour, filter, collecting decoction, being concentrated into 60 ℃ of relative densities is 1.05, adjusting contains the alcohol amount to 70%, cold preservation is spent the night, and reclaiming ethanol and being concentrated into 60 ℃ of relative densities is that 1.12-1.15 and above-mentioned alcohol extraction concentrated solution merge, add an amount of sucrose and water again, packing, high temperature sterilize, promptly.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102743662A (en) * | 2012-07-18 | 2012-10-24 | 广西中医药大学 | Zhuang medicine preparation for treating gynecological inflammation and preparation method thereof |
| CN105687491A (en) * | 2016-03-23 | 2016-06-22 | 黄群德 | Traditional Chinese medicine preparation for treating cancerous protuberance |
| CN106215118A (en) * | 2016-08-29 | 2016-12-14 | 广西康宝海美三高生态茶有限公司 | Antineoplastic health preparation and preparation method thereof |
| CN107029173A (en) * | 2017-01-16 | 2017-08-11 | 广西壮族自治区肿瘤防治研究所 | A kind of pharmaceutical composition for treating primary carcinoma of liver |
| CN109381516A (en) * | 2017-08-07 | 2019-02-26 | 北京蕴汇医药科技有限公司 | For preventing and treating the Chinese medicine composition and preparation method thereof of hepatic precancerosis and liver cancer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1891290A (en) * | 2006-03-06 | 2007-01-10 | 喻可安 | Medicine for treating cancer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1891290A (en) * | 2006-03-06 | 2007-01-10 | 喻可安 | Medicine for treating cancer |
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| 《中国实验方剂学杂志》 20021031 钟鸣等 复方红豆杉胶囊抗肿瘤药效学实验研究 45-47 1-8 第8卷, 第5期 * |
| 《中国实验方剂学杂志》 20110131 钟鸣等 壮药复方铁草胶囊毒理学研究 173-177 1-8 第17卷, 第1期 * |
| 《中国药师》 20100630 滕红丽 壮药铁包金的药学及临床应用研究进展 876-878 1-8 第13卷, 第6期 * |
| 《广西中医药》 20100430 钟鸣等 壮药"复方铁草胶囊"抗肿瘤作用的实验研究 54-56 1-8 第33卷, 第2期 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102743662A (en) * | 2012-07-18 | 2012-10-24 | 广西中医药大学 | Zhuang medicine preparation for treating gynecological inflammation and preparation method thereof |
| CN102743662B (en) * | 2012-07-18 | 2015-03-11 | 广西中医药大学 | Zhuang medicine preparation for treating gynecological inflammation and preparation method thereof |
| CN105687491A (en) * | 2016-03-23 | 2016-06-22 | 黄群德 | Traditional Chinese medicine preparation for treating cancerous protuberance |
| CN106215118A (en) * | 2016-08-29 | 2016-12-14 | 广西康宝海美三高生态茶有限公司 | Antineoplastic health preparation and preparation method thereof |
| CN107029173A (en) * | 2017-01-16 | 2017-08-11 | 广西壮族自治区肿瘤防治研究所 | A kind of pharmaceutical composition for treating primary carcinoma of liver |
| CN109381516A (en) * | 2017-08-07 | 2019-02-26 | 北京蕴汇医药科技有限公司 | For preventing and treating the Chinese medicine composition and preparation method thereof of hepatic precancerosis and liver cancer |
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