CN102940773A - Chinese medicinal preparation for inhibiting liver cancer metastasis and preparation method thereof - Google Patents

Abstract

The invention discloses a Chinese medicinal preparation for inhibiting liver cancer metastasis and a preparation method thereof. The preparation mainly comprises astragalus root, pilose asiabell root, bighead atractylodes rhizome, oldenlandia, root of red-rooted salvia, Chinese wolfberry, white peony root, dwarf lilyturf root, tortoise shell, turtle shell, dried orange peel, honey-fried licorice root and the like. The preparation method comprises the following steps of: milling the pilose asiabell root into powder, extracting each component by using water or ethanol, concentrating, combining, adding a filling agent, and preparing a solid preparation or a liquid preparation. The Chinese medicinal preparation has the effects of combining tonification and purgation, treating both symptoms and root causes, relieving pain, removing stasis, nourishing yin, nourishing blood, tonifying qi, restoring pulse, regulating the functions of human immune system and inhibiting the growth of cancer cells.

Description

Suppress Chinese medicine preparation of hepatoma Metastasis and preparation method thereof

Technical field

The present invention relates to a kind of Chinese medicine preparation that suppresses hepatoma Metastasis, particularly suppress Chinese medicine preparation of hepatoma Metastasis and preparation method thereof.

Background technology

Hepatocarcinoma (hepatocellular carcinoma, HCC) is the world's the 6th large tumor, and the HCC sickness rate is in rising trend in Europe and the U.S. in recent years, and China is the district occurred frequently.Chinese medicine prevention HCC is characteristic and the advantage of China treatment HCC, and Chinese traditional compound medicine is to the direct inhibition of HCC cell in recent years, on apoptotic impact, and rapid in the Advances in research on molecular mechanism that improves the aspects such as immunity of organisms; The clinical observation on the therapeutic effect research of Chinese traditional compound medicine prevention and treatment HCC also makes some progress, and the progress of existing nearly 5 years compound Chinese medicinal preparation control HCC is done a summary, provides reference for improving Chinese medicine early metaphase hepatocarcinoma.

Primary hepatocarcinoma is in the world the 6th modal cancer and the 3rd the modal cancer cause of the death.The primary hepatocarcinoma M ﹠ M of developing country is higher, and primary hepatocarcinoma that China occurs has just accounted for the over half of the total case of hepatocarcinoma in the world.Think that at present the drinking water of hepatitis B virus, food pollution AFB1, pollution and heavy drinking etc. are closely related with the generation of primary hepatocarcinoma.In today of industrial civilization prosperity, people are constantly in the face of various ambient pressures, environmental pollution, water, air and chemical fertilizer, pesticide, vehicle exhaust, food additive, unhygienic diet and often ediblely pickle food, the smoking of fire-cureing, drink, the factor affecting such as noise jamming and atmosphere radioactive substance.These various heterotoxins enter people's body, health risk by different approaches, and the disorder of body metabolic function, extremely easily the various refuses that produce in the metabolism are piled up and be stuck in the metabolism pipeline, stop up normal drain passage, consist of the endogenous toxin, bring out human body and produce various condition of illness.Finally can cause the various dysfunctions of body, such as the endocrine disturbance, degradation under lipid metabolism imbalance, the function of immune system is led oncogenic generation.

Because hepatocarcinoma is a class disease of serious threat human health, almost seldom can cure.Therefore, hepatocarcinoma is the emphasis of medical research always, seeks effective antitumour medicine and method, and thoroughly capturing hepatocarcinoma is the important subject of world medical circle.Studies show that many Chinese medicines have antihepatocarcinoma effect, especially compound recipe has multiple action component and produces antitumor action by multiple action pathway.Some medical facility and medical personnel have delivered the more open source literature report that utilizes Chinese herbal treatment hepatopathy or hepatocarcinoma in recent years, for example:

1, Chinese patent, title: a kind of anticancer dispersing tumor toxin expelling soup application (patent) number: the CN200410038090.X applying date: 2004.05.19, open (bulletin) number: CN1698684 open (bulletin) day: 2005.11.23 applies for (patent right) people: the big address of clock: (design) people invents in No. 89 Guangzhous, Linhe West Road, Guangzhou City, Guangdong Province out-patient department of Friendship Hospital: the big summary of clock: a kind of anticancer dispersing tumor toxin expelling soup, employing has heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, the Chinese medicine of anticancer dispersing tumor effect, wherein with Bufo siccus, ground beetle, Hirudo, Mylabris, the animal drugs such as Gekko Swinhonis are principal agent, are aided with the Radix Astragali, Radix Ginseng, Ganoderma, Radix Notoginseng, the Rhizoma Atractylodis Macrocephalae, rhizoma sparganic, Rhizoma Curcumae, Semen Persicae, Flos Carthami, the Chinese herbal medicine such as Herba Hedyotidis Diffusae are fried to be formed.The present invention is direct killing off tumor cells both, destroy the focus combination, but antiviral, antiinflammatory, destruction tumor vessel prevent cell transfer again, anti-cell suddenlys change, improves the cell means of information transmission, the drug resistance of reversing tumor cell, improve simultaneously body's immunity, thereby effectively treat various innocent and malignant tumours, especially Hepatoma therapy, pulmonary carcinoma, breast carcinoma best results.Principal claim: 1. anticancer dispersing tumor toxin expelling soup, the Chinese medicine decocting that employing has heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, anticancer dispersing tumor effect becomes, it is characterized in that: it is take animal drugs as principal agent, be aided with other Chinese herbal medicine and fry in shallow oil into, the concrete consumption of each medicine is Bufo siccus 5g, ground beetle 10g, Hirudo 2g, Mylabris 0.04g, Gekko Swinhonis 6g, Radix Astragali 30g, Radix Ginseng 10g, Ganoderma 15g, Radix Notoginseng 10g, Rhizoma Atractylodis Macrocephalae 20g, rhizoma sparganic 8g, Rhizoma Curcumae 8g, Semen Persicae 10g, Flos Carthami 6g, Herba Hedyotidis Diffusae 30g.

2, Chinese patent, title: a kind of anti-cancer Chinese medicinal powder and preparation method thereof application (patent) number: the CN02116298.0 applying date: 2002.04.01, open (bulletin) number: CN1448164, open (bulletin) day: 2003.10.15 applies for (patent right) people: Yang Wenxiu, address: the residence north 3-12 number invention in weaving 18-2 building, road (design) people: the Yang Wenxiu of Qiqihaer City, Heilongjiang Province Tiefeng District railway north office summary: a kind of anti-cancer Chinese medicinal powder and preparation method thereof, its Chinese powder medicine is comprised of the prescription II of prescription I bfore meals and one after each meal, and the component of its prescription I has Olibanum, Myrrha, Radix Angelicae Sinensis, Pericarpium Citri Reticulatae, Radix Et Rhizoma Rhei, Natrii Sulfas, Radix Paeoniae Rubra, Fructus Gleditsia, eupolyphage sece steleophaga, the pure Chinese medicine of 21 flavors such as Squama Manis.The component of its prescription II has the pure Chinese medicine of 13 flavors such as Radix Ginseng, the Rhizoma Atractylodis Macrocephalae, Poria, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Angelicae Pubescentis, Rhizoma Et Radix Notopterygii, the Radix Astragali, Radix Aconiti Lateralis Preparata.Its preparation method is: after the required pure Chinese medicine of prescription I is carried out respectively the rinsing of selected (not containing Natrii Sulfas) usefulness clear water by prescription, under 100 ℃ condition, dry, by ultraviolet disinfection 30 minutes, be broken into 120 order fine powders by the formula proportion mixed powder after the cooling, again the Natrii Sulfas component is developed into 120 order fine powders, after the fine powder mixing and stirring that crushes with above-mentioned prescription I medicine, incapsulate (0.5g/ grain), reinstall in the bottle and seal.Prescription II is without Natrii Sulfas, and its preparation method and prescription I are similar.This medicine has the immunity of organisms of raising, promotes metabolism, suppresses or the dissolved cancer cell function, and the kinds cancers such as hepatocarcinoma are had good therapeutic effect, and taking convenience has no side effect.Principal claim: 1. anti-cancer Chinese medicinal powder, it is characterized in that: it is comprised of the prescription II of prescription I bfore meals and one after each meal, its component proportion (weight portion) is as follows: prescription I (bfore meals): Olibanum 2.5-3%, Myrrha 2.5-3%, Radix Angelicae Sinensis 1.8-2% Pericarpium Citri Reticulatae 2.5-3%, Radix Et Rhizoma Rhei 6.4-6.8%, Natrii Sulfas 4.5-5% Radix Paeoniae Rubra 4.5-5%, Fructus Gleditsia 4.5-5%, eupolyphage sece steleophaga 5.4-5.8% Squama Manis 8-10%, Radix Angelicae Dahuricae 0.8-1%, pollen 2.5-3% Stigma Croci 4.5-5%, Flos Lonicerae 8-10%, Radix Aucklandiae 8-10% Radix Saposhnikoviae 1.8-2%, Pseudobulbus Bletillae (Rhizoma Bletillae) 8-10%, the Radix Bupleuri 1.3-1.5% core 4.5-5% that withers, the skin 4.5-5% that withers, Radix Notoginseng 4.5-5%; Prescription II (one after each meal): Radix Ginseng 8-9%, Rhizoma Atractylodis Macrocephalae 11-12%, Poria 8-9% Radix Glycyrrhizae 3-4%, Radix Angelicae Sinensis 3-4%, Radix Angelicae Pubescentis 4-4.5%, Rhizoma Et Radix Notopterygii 3-4%, Radix Astragali 17-18%, Radix Paeoniae Alba 11-12%, Radix Aconiti Lateralis Preparata 2.5-3%, Ramulus Cinnamomi 11-12%, bar halberd 5-6%, Semen Coicis 8-9%.

3, Chinese patent, title: a kind of Chinese medicine composition for the treatment of the liver cirrhosis disease and preparation method thereof, application (patent) number: the CN200910309349.2 applying date: 2009.11.05 open (bulletin) number: CN101869686A, open (bulletin) day: 2010.10.27 applies for (patent right) people: Thailand one and Pu (Beijing) Chinese medicine academy company limited, the address: 2027b, Building 9, No. 16, Fengguan Road, Fengtai District, Beijing City invention (design) people: Wang Feng summary: a kind of Chinese medicine composition for the treatment of the liver cirrhosis disease and preparation method thereof, Chinese medicine composition mainly are comprised of following crude drug: Sanguis Draxonis, Squama Manis, Rhizoma Chuanxiong, Radix Paeoniae Rubra, the Radix Paeoniae Alba, Poria, the Rhizoma Atractylodis Macrocephalae, Semen Persicae, Flos Carthami, Ganoderma, Sanguis Draxonis, Rhizoma Corydalis, Radix Angelicae Sinensis, rhizoma sparganic, Rhizoma Curcumae, Pericarpium Citri Reticulatae, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Ginseng, Radix Astragali, Radix Rehmanniae Preparata, Radix Puerariae, Colla Plastri Testudinis, Cornu Cervi Degelatinatum, Colla carapax Trionycis, Radix Rehmanniae, Radix Scrophulariae, Fructus Corni, the Radix Linderae, Fructus Aurantii, Rhizoma Cyperi, Pericarpium Citri Reticulatae, the Radix Aucklandiae, Lignum Aquilariae Resinatum, Cortex Magnoliae Officinalis, Fructus Evodiae, Bulbus Allii Macrostemonis, Aspongopus.Chinese medicine composition of the present invention can be prepared into according to the method for Chinese medicinal of routine any oral formulations commonly used.It is main that the present invention can significantly improve lassitude and weak, loss of appetite and dyspepsia, can have nauseating, to detest oil, abdominal part flatulence, epigastric discomfort, dull pain and diarrhoea, ordinary circumstance and nutriture relatively poor, it is weak to become thin, lassitude, serious symptom person is weak and be unable to leave the bed, and follow xerosis cutis coarse, complexion is gloomy pitch-dark, the symptoms such as anemia, glossitis, angular cheilitis, nyctalopia, polyneuritis and edema and water and electrolyte disturbance, portal hypertension, clinical efficacy is definite, evident in efficacy, take effect rapidly.

4, Chinese patent, application number: 201110039130.2 applyings date: 2011.02.17 title: Chinese medicine preparation that suppresses hepatocarcinoma and preparation method thereof is applied for (patent right) people: Deng Xin, address: invention (design) people: the Deng Xin of No. 10 Rui Kang hospitals in East China road, Nanning City, Guangxi Zhuang Autonomous Region; Liang Jian; Wu Fasheng; Zhang Yaping summary: the present invention relates to a kind of Chinese medicine preparation that suppresses hepatocarcinoma and preparation method thereof, its raw material comprises Radix Ginseng, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Carapax et Plastrum Testudinis, Herba Hedyotidis Diffusae, Carapax Trionycis, the Radix Paeoniae Alba, Radix Aconiti Lateralis Preparata, the Cortex Eucommiae and Radix Glycyrrhizae Preparata etc., preparation method is to get Radix Ginseng, process and sweetly be crushed into powder respectively, again with each component water or ethanol extraction, concentrated, merge, add filler, make solid preparation or liquid preparation product, this Chinese medicine preparation reinforcement and elimination in combination, giving consideration to both the incidental and fundamental, the medicinal powder for relieving pain knot, play altogether nourishing YIN and benefiting blood, Yiqi and vein recovery and balance the body function of immune system are to the opposing tumor, keep the toxin expelling pipeline unimpeded, the growth of anticancer all has effect.Because Chinese medicine composition of the present invention has adopted the integration of edible and medicinal herbs medicine of state-promulgated pharmacopoeia regulation to make up substantially, so have cheap, the basic advantage such as have no side effect of expense.

Summary of the invention

For reaching this purpose, the inventor studies Traditional Chinese medical theory, on the basis of secret prescription handed down in the family from generation to generation and former patent 201110039130.2, replenished and put in order, excavate and also to develop a kind of adjusting viscera function imbalance, the inhibition hepatoma Metastasis that expense is cheap, have no side effect substantially Chinese medicine preparation and preparation method thereof.

The present invention suppress hepatoma Metastasis the Chinese medicine preparation effective ingredient be to be made by the raw material of Chinese medicine of following parts by weight:

Radix Astragali 10-30;

Radix Codonopsis 10-20;

Rhizoma Atractylodis Macrocephalae 5-15;

Herba Hedyotidis Diffusae 20-40;

Radix Salviae Miltiorrhizae 5-15;

Fructus Lycii 10-20;

Radix Paeoniae Alba 5-15;

Radix Ophiopogonis 5-15;

Carapax et Plastrum Testudinis 10-25;

Carapax Trionycis 10-25;

Pericarpium Citri Reticulatae 5-15;

Radix Glycyrrhizae Preparata 5-15.

According to Chinese medicine document record, being analyzed as follows of above-described each composition of Chinese medicine:

The Radix Astragali, latin name: Leguminosae invigorating QI to consolidate the body surface resistance, diuresis poison holding, QI invigorating invigorating middle warmer.The Radix Astragali is leguminous plant, and it is the Chinese medicine of commonly using simply.Its main pharmacological is " benefiting QI for strengthening the superficies ", can " diuretic ", and also can " promoting pus discharge and tissue regeneration strengthening ".

Radix Codonopsis, another name: Shangdang Radix Ginseng, Radix Saposhnikoviae Radix Codonopsis, Sphallerocarpus gracilis, preventing lanceolata, upper Radix Codonopsis, SHITOUSEN, middle Ganoderma, yellow party, the Codonopsis plant, campanulaceae, English name: Root of Pilose Asiabell, Pilose Asiabell Root Moderate Asiabell Root Szechwon Tangshen Root, Latin formal name used at school: Root of Pilose Asiabell, meridian distribution of property and flavor: property is flat, sweet in the mouth, little acid.Return spleen, lung meridian.Has invigorating the spleen and replenishing QI, the effect of spleen invigorating lung benefiting.Cure mainly weakness of the spleen and stomach, anorexia and loose stool, fatigue and weakness, the deficiency of the lung is breathed with cough, the lazy speech of breathing hard, spontaneous perspiration, blood deficiency and yellow complexion, thirsty.Be used for deficiency of the spleen and lung, the cardiopalmus of breathing hard, anorexia and loose stool, the dyspnea due to deficiency cough, interior-heat is quenched one's thirst etc." Bencao Congxin " record: " invigorating the spleen and replenishing QI and taste, relieving thirst and restlessness.A little less than the middle feeble QI, mend in order to transfer, very smooth and proper.”

The Radix Astragali and Radix Codonopsis all belong to QI invigorating good medicine, but Radix Codonopsis biases toward strongly invigorating primordial QI, and recuperating depleted YANG and rescuing the patient from collapse is usually used in the emergency cases such as collapse, shock, and effect is better.The Radix Astragali is then take tonify deficiency as main, be usually used in asthenia with the passing of time, low, the thready pulse without strength person of speech.Radix Astragali tool and mending and oiliness characteristics, and if Radix Codonopsis, the tonic compatibilities such as Radix Ginseng are better effects if then.But Radix Astragali single uses, and also can use with the other medicines compatibility.

The Rhizoma Atractylodis Macrocephalae, latin name: Rhizoma Atractylodis Macrocephalae, function cures mainly: invigorating the spleen and benefiting QI, the dampness diuretic, hidroschesis, antiabortive.Be used for insufficiency of the spleen lack of appetite, abdominal distention is had loose bowels, phlegm retention vertigo and palpitation, edema, spontaneous perspiration, frequent fetal movement.It is reported that mouse stomach Rhizoma Atractylodis Macrocephalae decocting liquid can be prevented and treated the hepatic injury due to the carbon tetrachloride, alleviate hepatic glycogen minimizing and hepatocellular degeneration necrosis, promote hepatocyte to increase, the ALT of rising is descended.

Herba Hedyotidis Diffusae (formal name used at school: Hedyotis diffusa) be the plant of Rubiaceae cerastium.Its Chinese patent medicine bitter in the mouth, light, cold in nature.Main effect is heat-clearing and toxic substances removing, medicinal powder for relieving pain knot, promoting urination to remove dampness.Outstanding kind treatment all kinds inflammation has stronger inhibitory action at external (quite crude drug 6g/ml) to the tumor cell of acute lymphoblastic type, granulocyte type, monocyte type and chronic granulocyte type.

Radix Salviae Miltiorrhizae, [another name] blood is taken root, Radix Salviae Miltiorrhizae, Herba Wedeliae Wallichii, Arisaema balansae Engl. [English name] Dan one ShenRoot[Latin] Radix Salviae Miltiorrhizae; Be the dicotyledon Labiatae, dry root and rhizome, function cures mainly: stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, relieving restlessness clears away heart-fire.Be used for that menoxenia, amenorrhea dysmenorrhea, lump in the abdomen, breast ventral spine pain, pyretic arthralgia pain, skin infection are swollen and ache, hepatosplenomegaly, angina pectoris.

Fructus Lycii, [another name] der Bockdorn, the young thorn of red pearl, the right power of cattle, Canis familiaris L. serratd edge, it is the multi-branched shrub plant of Solanaceae Lycium, [English name] Barbary Wolfberry Fruit, [Latin] Lycium chinense has the kidney invigorating and essence nourishing, nourishing the liver to improve visual acuity, blood-enriching tranquillizing, promoting the production of body fluid to quench thirst, nourishing the lung to arrest cough.Control hepatic and renal YIN deficiency, soreness of the waist and knees, dizziness, dizzy, the many tears of blurred vision, cough due to consumptive disease is quenched one's thirst, seminal emission.

The Radix Paeoniae Alba; Radix Paeoniae Alba (formal name used at school: Paeonia sterniana Fletcher in Journ.) also claim Paeonia sterniana Fletcher in Journ.; it is Ranunculaceae Paeonia plant; pharmacological action: 1; effect to cardiovascular system: the Radix Paeoniae Alba has coronary artery dilator; hypotensive action (d-cachou extract and progallin A have antithrombotic and antiplatelet aggregative activity); 2; liver protection effect: the Radix Paeoniae Alba is to carbon tetrachloride; AFB1; hepatic injury has obvious protective effect due to the D-galactosamine; 3; spasmolysis: the Radix Paeoniae Alba has inhibitory action to intestinal tube and stomach in place motion; can significantly resist the uterine contraction that oxytocin causes; 4; analgesic activity: the Radix Paeoniae Alba can suppress mouse writhing; shout; the hot plate reaction; morphine is suppressed writhing response synergism is arranged; and can resist and faint from fear 5 due to the pentylenetetrazole; antibacterial action.The Radix Paeoniae Alba has protective effect to liver; Radix Paeoniae Alba extract raises to hepatic injury due to the D-galactosamine and SGPT obvious antagonism; SGPT is reduced; and making hepatocellular pathological changes and downright bad recovery normally. the lactic acid dehydrogenase that the acute liver injury of rats that the ethanol extraction of the Radix Paeoniae Alba can make aflatoxin B1 cause shows and the gross activity of isozyme raise and are minimized. and white peony root's total glycoside can suppress carbon tetrachloride induced mice Plasma glutamate-pyruvate transaminase and lactic acid dehydrogenase raises; and liver organization bitten acid denaturation; necrosis has certain antagonism. and the someone thinks; hepatic injury due to the carbon tetrachloride is to destroy because of membrane structure generation peroxidation; make glutamate pyruvate transaminase and lactic acid dehydrogenase rising in the blood; white peony root's total glycoside may have protective effect to hepatocyte injury, therefore the transaminase is reduced.

Radix Ophiopogonis, have another name called Radix Ophiopogonis, dwarf lilyturf, book band grass; Liliaceae Ophiopogon perennial evergreen herbaceous plant; The Latin formal name used at school: O.japonicus(L. f.) Ker-Gawl., meridian distribution of property and flavor: sweet, little hardship is slightly cold.GUIXIN, lung, stomach warp.The sick impairment of body fluid of effect 1. replenishing YIN and removing heat treatment yin asthenia generating intrinsic heat or calentura, restlessness and thirst.2. thick, the QI rising in reverse order of cough, expectorant due to the nourishing the lung to arrest cough treatment dryness-heat impairing the lung.Function cures mainly: YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire.Be used for dryness of the lung dry cough.The chronic consumptive disease cough, Tianjin wound is thirsty, vexed insomnia, interior-heat is quenched one's thirst, the dryness of the intestine constipation; Pharyngeal diphtheria.Control dryness of the lung dry cough, spit blood, spitting of blood, consumptive lung disease, lung abscess, the asthenia dysphoria with smothery sensation is quenched one's thirst, and hinder in calentura Tianjin, dry mouth and throat, constipation.

Carapax et Plastrum Testudinis is sternite and the carapace of Testudinidae animal chinemys reevesii.Function is the nourishing kidney YANG hyperactivity suppressing, replenishing kidney and strengthening bone, the reinforcing the heart of nourishing blood.Cure mainly: 1. hyperactivity of YANG due to deficiency of YIN, yin asthenia generating intrinsic heat, stirring-up of pathogenic wind in the interior resulting from deficiency.2. the atrophic debility of bones of suffering from a deficiency of the kidney, infantile metopism.3. deficiency of YIN-blood is palpitated with fear, and insomnia is forgetful.Effect: the effect to immunologic function experimental results show that: Testudinis deer oral liquor for spleen and kidney and pill are oral to mice, can improve the phagocytic function of its reticuloendothelial system, significantly increase phagocytic index and engulf coefficient; The spleen that makes mice under normal and the immunosuppressive condition, the effect of thymus weightening finish are also arranged; the tip leukopenia that cyclophosphamide is caused has certain protective effect. bibliographical information; Carapax et Plastrum Testudinis has antitumor action can improve body antineoplastic immunocompetence, and its extract has inhibitory action to sarcoma S180, ehrlich ascites tumor and ascitic type liver cancer.

Carapax Trionycis Carapax Amydae is the carapace of Trionychidae animal Trionyx sinensis Wiegmann Trionyx sinensis Wiegmann.Meridian distribution of property and flavor: salty, be slightly cold.Return liver, kidney channel.Function cures mainly: nourishing YIN for suppressing the hyperactive YANG, hard masses softening and resolving is brought down a fever except steaming.Be used for fever due to yin deficiency, consumptive fever hectic fever due to YIN-deficiency, stirring-up of pathogenic wind in the interior resulting from deficiency, amenorrhea ， WEIJIA, chronic malaria malaria with splenomegaly.Have report to point out, the former powder of Trionyx sinensis Wiegmann and Turtle Shell have inhibitory action to transplanting solid carcinoma, and the tumor diameter is significantly reduced, and tumor weight significantly alleviates; But to ehrlich ascites carcinoma then without remarkable effect. also have the report point out, be the aqueous solution supernatant of the main loose pill of herbal mixture Carapax Trionycis in order to Carapax Trionycis, after quantitatively inoculating for cancerous cell, observe it to the effect situation of growth of cancer cells, the result shows: the loose supernatant of Carapax Trionycis all has inhibitory action to mouse lymphocyte leukemia (L1210), HL-60 and gastric cancer 803 Growth of Cells.

Pericarpium Citri Reticulatae is rutaceae, [different name] Pericarpium Citri tangerinae Shennong's Herbal, Fructus Citri tangerinae skin " chicken peak Prescriptions for Universal Relief ", Folium Styracis Suberifoliae Materia Medica of Decoction, orange peel " the southern regions of the Yunnan Province book on Chinese herbal medicine ", wide Pericarpium Citri tangerinae " proper book on Chinese herbal medicine ".[phonetic]: Chenpi[is English] Dried Tangerine Peel[Latin] Pericarpium Citri Reticulatae[nature and flavor, return through] hot, bitter, temperature.Return board, lung meridian.[effect of Pericarpium Citri Reticulatae] 1. is used for the diseases such as distension and fullness of the chest and abdomen.The hot loose logical temperature of Pericarpium Citri tangerinae, fragrant odour is longer than and is regulated the flow of vital energy, and can enter the spleen lung, thus can go loose sluggishness of lung-QI, again can promoting the circulation of QI to alleviate the stagnation in middle-JIAO, be used for lung qi and gather around the diseases such as stagnant, chest and diaphragm feeling of fullness and stagnation of QI in spleen and stomach, distension and fullness in the abdomen.Normal and the compatibility application such as the Radix Aucklandiae, Fructus Aurantii.2. be used for retention of dampness in middle-JIAO, gastral cavity abdomen painful abdominal mass is swollen, have loose bowels in the loose stool, and the disease such as abundant expectoration cough.The Pericarpium Citri tangerinae dispelling the damp pathogen with bitter and warm drugs and can spleen invigorating circulation of qi promoting, so be usually used in the diseases such as retention of dampness in middle-JIAO, gastral cavity abdominal distention are vexed, loose stool greasy fur, but compatibility Cang Intraoperative, Cortex Magnoliae Officinalis are with using.Be good at again drying dampness to eliminate phlegm, stop up the key medicine commonly used of lung, abundant expectoration cough for controlling damp-phlegm, every and the Rhizoma Pinelliae, Poria are used together.3. be used for that insufficiency of the spleen diet reduces, dyspepsia, and the disease such as nausea and vomiting.This product dampness and can spleen benefiting and stimulating the appetite is applicable to the diseases such as weakness of the spleen and stomach, diet minimizing, dyspepsia, loose stool and diarrhea, the fit applications such as normal and Radix Ginseng, Bai Intraoperative, Poria.Can spleen invigorating because of it, can regulate the flow of vital energy again, so often make as the assistant of Qi-tonifying drug, can make tonify without causing stagnation, prevent from stopping up the swollen effect of doing of holding back.In addition, Pericarpium Citri tangerinae again can with in, can control stomach-QI being unable to descend normally, nausea and vomiting, if gastrofrigid vomiting, can with Rhizoma Zingiberis Recens with using; Gastropyretic vomiting, but the medicines such as compatibility Caulis Bambusae In Taenia, Rhizoma Coptidis are used together again.The merit of appetizing in [effect of Pericarpium Citri Reticulatae] tool regulating QI and lowering the adverseness, the accent, drying dampness to eliminate phlegm.Cure mainly dirty contrary, the difficulty in urination and defecation of full vexed, the abdominal distention of the wet resistance of stagnation of QI in spleen and stomach, chest and diaphragm, anorexia, vomiting, lung qi retardance, cough with copious phlegm, also control acute mastitis from the beginning of.Through for many years research, existing known Pericarpium Citri Reticulatae has many pharmacological actions.

Radix Glycyrrhizae Preparata (RADIX GLYCYRRHIZAE PREPARATA) is leguminous plant, and Radix Glycyrrhizae Preparata is the Radix Glycyrrhizae of smoking with honey.Function with cure mainly: spleen reinforcing stomach function regulating, Yiqi and vein recovery.Be used for weakness of the spleen and stomach, fatigue and weakness, severe palpitation, irregularly intermittent and regularly intermittent pulse can be separated the Radix Aconiti Lateralis Preparata poison.Main effect: nourishing YIN, enrich blood, negative and positive and transferring.

More than all medicines share reinforcement and elimination in combination, medicinal powder for relieving pain knot, giving consideration to both the incidental and fundamental, play altogether nourishing YIN and benefiting blood, Yiqi and vein recovery, promoting blood circulation and stopping pain and adjusting digestive system and human immune system's function, to resisting tumor, keeping the toxin expelling pipeline unimpeded, the growth of anticancer all has effect.

The Chinese herbal treatment malignant tumor, the determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs of foot wholistic therapy phase, namely entire and part combines, has both seen that body is to the defensive ability/resistance ability of pathogenic factor, see that again local patholoic change is on the impact of body, on differential diagnosis of diseases and dialectical basis, by the composition of prescription of some specific drugs with strong points, enter rightly body, regulate the balance of QI and blood, negative and positive, body fluid.Drug molecule enters whole body through physical reactions and chemical reaction, and killing cancer cell is enclosed in the surface that is adsorbed on cell that has, corrosion, and this can be called " battle of encirclement "; The medicament composing prescription that has by different effects, is cut apart cancerous cell, defeats in detail again, and this can be called " fighting war "; The effect of passing through medicine itself that has is accompanied by synergistic medicine, can tumoricidal blood circulation, and remove and change the blood fortune of loving the body part, the propagation of control cancerous cell, this can be called " instigating rebellion within enemy camp war "; Have in conjunction with body condition, transfer, strengthen, improve the function of immune cell by medicine and remove to engulf cancerous cell, this can be called " immunity war "; The nature and flavor that pass through medicine that have add and use local application, go to destroy structure and the generation of collapsing cell with temperature, and this can be called " jamming war "; The medicine that has can be regulated QI and blood, can make QI-blood circulation normal, and the isolation cancerous cell makes it to excrete, and this can be called " expulsion war "; What have can affect the cancerous cell endoenzyme: make it become " end fat ", make cancerous cell chromosome, gene necrosis, this can be called " sabotage operations "; Can help immunocyte chromosome, the gene that have restore, and this can be called " drawing war over to one's side "; What have can make QI and blood energy mean allocation. hungry cancerous cell, make it to revert to normal cell, and this can be called " balance war ".

So, Chinese medicine preparation of the present invention has been followed whole existing the thought and the determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs principle of motherland's medical science, inquire into the rule of malignant tumor genesis from Molecular level study. and adopt blood circulation promoting and blood stasis dispelling, hard masses softening and resolving, heat-clearing and toxic substances removing, the body resistance strengthening and constitution consolidating etc. to have " operation " means of TCM Features, accomplish " medication is as using military forces ", ability " To know one's own strength and the enemy's is the sure way to victory ", in the assault of fortified positions of going on an expedition cancer, handy, set up outstanding service, make Chinese medicine demonstrate immeasurable great function.

Chinese medicine preparation of the present invention and the applicant's 201110039130.2 applyings date of Chinese patent: 2011.02.17 title: the technology contents that suppresses Chinese medicine preparation and preparation method thereof of hepatocarcinoma is compared, and medicine has increased several: Radix Codonopsis, Fructus Lycii, Radix Ophiopogonis, Radix Salviae Miltiorrhizae and Pericarpium Citri Reticulatae.So that whole Chinese medicine has invigorating the spleen and replenishing QI, the spleen invigorating lung benefiting, YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire, stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, the sharp spleen of the liver benefiting, hot loose logical temperature, fragrant odour, be longer than and regulate the flow of vital energy, can enter the effects such as spleen lung, controlled the hepatocarcinoma continued growth, just can spread to other organ metastasis by anticancer.

The present invention suppress hepatoma Metastasis Chinese medicine preparation can add filler or the solution that meets medical standard, make medically solid preparation or liquid preparation, such as tablet, capsule, granule, pill, oral liquid or syrup etc., below introduce respectively the preparation method of several dosage forms:

Described solid preparation preparation method is as follows:

Get Radix Codonopsis and be crushed into powder, for subsequent use; With Carapax et Plastrum Testudinis, Carapax Trionycis places the copper pot, adds water logging and does not have face, heats with slow fire, keep little and boiled 5-12 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, until abundant fried glue juice is degree, then filter clarification, merge glue that each time fried in shallow oil, remove by filter precipitated impurities, the concentrated glue of receiving, glue juice after filtering is put in the copper pot concentrated with the slow fire heating, constantly stirred, in case coking, to be concentrated to " extension flag " the rear glue of receiving, in glue impouring gel dish, make it natural condensation, solidify for accelerating glue juice, also can add the 5%-10% Colla Corii Asini, good blob of viscose is solidified in taking-up, cuts into pieces with cutting the glue cutter, and is for subsequent use.The Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, Radix Salviae Miltiorrhizae, Fructus Lycii, the Radix Paeoniae Alba, Radix Ophiopogonis, Pericarpium Citri Reticulatae and Radix Glycyrrhizae Preparata volumetric concentration are the ethanol extraction twice of 50-90%, and merge extractive liquid, is for subsequent use; Concentrated after filtering, Recycled ethanol then adds Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, and concentrated, gets dry extract, merges with above-mentioned Radix Codonopsis powder again, and mix homogeneously adds filler, makes capsule or granule, tablet, obtains product; Perhaps add sucrose or Mel, mix homogeneously is made pill.

The preparation method of above-described oral liquid is to get Radix Codonopsis, Radix Glycyrrhizae Preparata, adds respectively decocting in water 2-4 hour, and is for subsequent use; With Carapax et Plastrum Testudinis, Carapax Trionycis places the copper pot, adds water logging and does not have face, heats with slow fire, keep little and boiled 5-12 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, until abundant fried glue juice is degree, then filter clarification, merge glue that each time fried in shallow oil, remove by filter precipitated impurities, reconcentration is received glue, glue juice after filtering is put in the copper pot concentrated with the slow fire heating, constantly stirred, in case coking, to be concentrated to " extension flag " the rear glue of receiving, in glue impouring gel dish, make it natural condensation, solidify for accelerating glue juice, also can add 5 %-10 % Colla Corii Asini, good blob of viscose is solidified in taking-up, cuts into pieces with cutting the glue cutter, and is for subsequent use.The Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, Radix Salviae Miltiorrhizae, Fructus Lycii, the Radix Paeoniae Alba, Radix Ophiopogonis and Pericarpium Citri Reticulatae volumetric concentration are the ethanol extraction twice of 50-90%, and merge extractive liquid, is for subsequent use; Recycled ethanol after filtering, concentrated, then, add Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, also concentrated, dry extract, merge with above-mentioned Radix Codonopsis, Radix Glycyrrhizae Preparata solution again, add the proper amount of edible antiseptic, with the pure water that adds the total drug weight 3-5 of dry extract times, make oral liquid, obtain product.

The invention has the beneficial effects as follows:

The present invention treats the Chinese medicine preparation reinforcement and elimination in combination of early metaphase hepatocarcinoma, giving consideration to both the incidental and fundamental, and the medicinal powder for relieving pain knot is played nourishing YIN and benefiting blood, Yiqi and vein recovery and balance the body function of immune system altogether, and the growth of anticancer all has effect.

 

The specific embodiment

Following embodiments of the invention:

Embodiment one:

Get 10 kilograms of Radix Codonopsis and be crushed into powder, for subsequent use; With 10 kilograms of Carapax et Plastrum Testudiniss, 10 kilograms of Carapax Trionycis place the copper pot, add water logging and do not have face, heat with slow fire, keep little and boiled 5-12 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, until abundant fried glue juice is degree, then filter clarification, merge glue that each time fried in shallow oil, remove by filter precipitated impurities, the concentrated glue of receiving, glue juice after filtering is put in the copper pot concentrated with the slow fire heating, constantly stirred, in case coking, to be concentrated to " extension flag " the rear glue of receiving, in glue impouring gel dish, make it natural condensation, solidify for accelerating glue juice, also can add the 5%-10% Colla Corii Asini, good blob of viscose is solidified in taking-up, cuts into pieces with cutting the glue cutter, and is for subsequent use.5 kilograms of 10 kilograms of the Radixs Astragali, 5 kilograms of the Rhizoma Atractylodis Macrocephalaes, 20 kilograms of Herba Hedyotidis Diffusaes, 5 kilograms of Radix Salviae Miltiorrhizaes, 10 kilograms of Fructus Lyciis, 5 kilograms of the Radix Paeoniae Albas, 5 kilograms of Radix Ophiopogonis, 5 kilograms of Pericarpium Citri Reticulataes and Radix Glycyrrhizae Preparatas are twice of the ethanol extraction of 50-90% with volumetric concentration, merge extractive liquid,, for subsequent use; Concentrated after filtering, Recycled ethanol then adds Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, and concentrated, gets dry extract, merges with above-mentioned Radix Codonopsis powder again, and mix homogeneously adds filler, makes capsule.

Embodiment two:

Get 12 kilograms of Radix Codonopsis and be crushed into powder, for subsequent use; With 13 kilograms of Carapax et Plastrum Testudiniss, 13 kilograms of Carapax Trionycis place the copper pot, add water logging and do not have face, heat with slow fire, keep little and boiled 5-12 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, until abundant fried glue juice is degree, then filter clarification, merge glue that each time fried in shallow oil, remove by filter precipitated impurities, the concentrated glue of receiving, glue juice after filtering is put in the copper pot concentrated with the slow fire heating, constantly stirred, in case coking, to be concentrated to " extension flag " the rear glue of receiving, in glue impouring gel dish, make it natural condensation, solidify for accelerating glue juice, also can add the 5%-10% Colla Corii Asini, good blob of viscose is solidified in taking-up, cuts into pieces with cutting the glue cutter, and is for subsequent use.7 kilograms of 14 kilograms of the Radixs Astragali, 7 kilograms of the Rhizoma Atractylodis Macrocephalaes, 25 kilograms of Herba Hedyotidis Diffusaes, 7 kilograms of Radix Salviae Miltiorrhizaes, 13 kilograms of Fructus Lyciis, 7 kilograms of the Radix Paeoniae Albas, 7 kilograms of Radix Ophiopogonis, 7 kilograms of Pericarpium Citri Reticulataes and Radix Glycyrrhizae Preparatas are twice of the ethanol extraction of 50-90% with volumetric concentration, merge extractive liquid,, for subsequent use; Concentrated after filtering, Recycled ethanol then adds Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, and concentrated, gets dry extract, merges with above-mentioned Radix Codonopsis powder again, and mix homogeneously adds filler, makes tablet.

Embodiment three:

Get 15 kilograms of Radix Codonopsis and be crushed into powder, for subsequent use; With 15 kilograms of Carapax et Plastrum Testudiniss, 15 kilograms of Carapax Trionycis place the copper pot, add water logging and do not have face, heat with slow fire, keep little and boiled 5-12 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, until abundant fried glue juice is degree, then filter clarification, merge glue that each time fried in shallow oil, remove by filter precipitated impurities, the concentrated glue of receiving, glue juice after filtering is put in the copper pot concentrated with the slow fire heating, constantly stirred, in case coking, to be concentrated to " extension flag " the rear glue of receiving, in glue impouring gel dish, make it natural condensation, solidify for accelerating glue juice, also can add the 5%-10% Colla Corii Asini, good blob of viscose is solidified in taking-up, cuts into pieces with cutting the glue cutter, and is for subsequent use.10 kilograms of 18 kilograms of the Radixs Astragali, 10 kilograms of the Rhizoma Atractylodis Macrocephalaes, 28 kilograms of Herba Hedyotidis Diffusaes, 10 kilograms of Radix Salviae Miltiorrhizaes, 15 kilograms of Fructus Lyciis, 10 kilograms of the Radix Paeoniae Albas, 10 kilograms of Radix Ophiopogonis, 10 kilograms of Pericarpium Citri Reticulataes and Radix Glycyrrhizae Preparatas are twice of the ethanol extraction of 50-90% with volumetric concentration, merge extractive liquid,, for subsequent use; Concentrated after filtering, Recycled ethanol then adds Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, and concentrated, gets dry extract, merges with above-mentioned Radix Codonopsis powder again, and mix homogeneously adds filler, the granulation agent.

Embodiment four:

Get 18 kilograms of Radix Codonopsis and be crushed into powder, for subsequent use; With 20 kilograms of Carapax et Plastrum Testudiniss, 20 kilograms of Carapax Trionycis place the copper pot, add water logging and do not have face, heat with slow fire, keep little and boiled 5-12 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, until abundant fried glue juice is degree, then filter clarification, merge glue that each time fried in shallow oil, remove by filter precipitated impurities, the concentrated glue of receiving, glue juice after filtering is put in the copper pot concentrated with the slow fire heating, constantly stirred, in case coking, to be concentrated to " extension flag " the rear glue of receiving, in glue impouring gel dish, make it natural condensation, solidify for accelerating glue juice, also can add the 5%-10% Colla Corii Asini, good blob of viscose is solidified in taking-up, cuts into pieces with cutting the glue cutter, and is for subsequent use.12 kilograms of 25 kilograms of the Radixs Astragali, 12 kilograms of the Rhizoma Atractylodis Macrocephalaes, 30 kilograms of Herba Hedyotidis Diffusaes, 12 kilograms of Radix Salviae Miltiorrhizaes, 18 kilograms of Fructus Lyciis, 12 kilograms of the Radix Paeoniae Albas, 12 kilograms of Radix Ophiopogonis, 12 kilograms of Pericarpium Citri Reticulataes and Radix Glycyrrhizae Preparatas are twice of the ethanol extraction of 50-90% with volumetric concentration, merge extractive liquid,, for subsequent use; Concentrated after filtering, Recycled ethanol then adds Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, and concentrated, gets dry extract, merges with above-mentioned Radix Codonopsis powder again, and mix homogeneously adds filler, makes pill.

Embodiment five:

Get 20 kilograms of Radix Codonopsis and be crushed into powder, for subsequent use; With 25 kilograms of Carapax et Plastrum Testudiniss, 25 kilograms of Carapax Trionycis place the copper pot, add water logging and do not have face, heat with slow fire, keep little and boiled 5-12 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, until abundant fried glue juice is degree, then filter clarification, merge glue that each time fried in shallow oil, remove by filter precipitated impurities, the concentrated glue of receiving, glue juice after filtering is put in the copper pot concentrated with the slow fire heating, constantly stirred, in case coking, to be concentrated to " extension flag " the rear glue of receiving, in glue impouring gel dish, make it natural condensation, solidify for accelerating glue juice, also can add the 5%-10% Colla Corii Asini, good blob of viscose is solidified in taking-up, cuts into pieces with cutting the glue cutter, and is for subsequent use.15 kilograms of 30 kilograms of the Radixs Astragali, 15 kilograms of the Rhizoma Atractylodis Macrocephalaes, 40 kilograms of Herba Hedyotidis Diffusaes, 15 kilograms of Radix Salviae Miltiorrhizaes, 20 kilograms of Fructus Lyciis, 15 kilograms of the Radix Paeoniae Albas, 15 kilograms of Radix Ophiopogonis, 15 kilograms of Pericarpium Citri Reticulataes and Radix Glycyrrhizae Preparatas are twice of the ethanol extraction of 50-90% with volumetric concentration, merge extractive liquid,, for subsequent use; Concentrated after filtering, then Recycled ethanol adds Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, also concentrated, gets dry extract, and mix homogeneously adds the proper amount of edible antiseptic, and adds the pure water of 5 times of the total drug weight of dry extract, makes oral liquid, obtains product.

Chinese medicine preparation of the present invention suppresses the hepatocarcinoma test:

Below be the application example of Chinese medicine preparation of the present invention:

One, the anticancer experiment in vitro that carries out of the medicine of the present invention made of the embodiment of the invention:

The summary purpose:With the inhibition situation of cell in vitro culture method research drug extract of the present invention to human hepatoma cell strain Hele7404 growth. The result:Drug extract of the present invention has inhibitory action to the growth of human hepatoma cell strain Hele7404.Drug level is lower than (0.5 μ g/ml), and is not obvious to the effect of tumor cell.Along with drug level is increased to 5.0 μ g/ml, the growth of tumor cell line is subject to obvious inhibition. Conclusion:Drug extract of the present invention has a function of tumor inhibition external.

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Materials and methods

1 material

1.1 drug extract concentrating part of the present invention, medicinal liquid are for subsequent use with aseptic filtration after the DMSO dissolving before use.

1.2 tumor cell line human hepatoma cell strain Hele7404 is provided by pharmacology teaching and research room of Guangxi Medical University.

1.3 reagent MTT(tetramethyl tetrazolium bromide) be German Sigma company product, lot number: 020609; FBS(embryo Ox blood serum) is U.S. Hyclone company product, lot number: 0405; RPMI1640 and DMEM culture medium are U.S. GIBCO company product, lot number: 1120708 and 0311; The Trypsin(trypsin) provides lot number by Tianjin Hao ocean biological product Co., Ltd: 200503; The DMSO(dimethyl sulfoxide) provides lot number by Tianjin remittance English chemical reagent company limited: 20030526.

1.4 experimental apparatus CO ₂Incubator (ThermO Forma), the U.S. produces.Inverted microscope (Olympus), Japan produces.Microplate reader (Biocell), Australia's land productivity produces.

2 experimental techniques

2.1 mtt assay list of references report, every hole adds 200 μ l(and contains 5000/ml tumor cell in 96 well culture plates (NUNC)) contain the RPMI1640 culture medium of 10%FBS cell suspension (except the NG108-15 cell with containing the DMEM culture medium of 10%FBS), put 37 ℃ of 10%CO ₂After cultivating 24h in the incubator, experimental group adds respectively the culture fluid of the Semen livistonae chinensis extract of ultimate density 0.5 ug/ml, 1.0ug/ ml, 5.0ug/ ml, and matched group then adds the culture fluid of isoconcentration equal-volume solvent, and repeat 4 times in every group of 4 holes.Put 37 ℃, 10%CO ₂Incubator was cultivated after 5 days, abandoning supernatant, the serum-free medium that adds the freshly prepared 0.2mg/ml of the containing MTT in 200 μ l/ holes, 37 ℃ are continued to cultivate 4h. and abandon supernatant, add 200 μ l DMSO, behind the vibration mixing, take wavelength as 550nm, reference wavelength is that 450 nm measure the OD value on microplate reader. result of calculation.

Be calculated as follows medicine to the suppression ratio of growth of tumour cell.

Growth of tumour cell suppression ratio %=(the average OD value of 1-experimental group/contrast average OD value) * 100%

The attached cell of trophophase 2.2 colony forming method is taken the logarithm (Hela, SGC7901, B16) is used 10%FBS

The RPMI1640 culture fluid and the DMEM culture fluid of NG108 – 15 usefulness 10%FBS be made into 500/ml cell suspension, be inoculated in the 35mm culture dish (NUNC), 4 is one group, each 2ml; It is the cell suspension 200ul of the Semen livistonae chinensis extract of 0.5 ug/ml, 1.0ug/ ml, 5.0ug/ ml that experimental group adds final concentration.The cell suspension 200ul. that matched group adds isoconcentration equal-volume solvent puts 5%CO ₂37 ℃ of incubators in leave standstill 7d; Discard culture fluid, contain the above cell colony of 50 cells with counting after Rui Shi-Ji's nurse Sa dyeing.To suspension cell (L1210 and P388D ₁) be made into 1000/ml cell suspension with the RPMI1640 culture fluid of 10%FBS, put 37 ℃ of pre-temperature; Get 35mm culture dish (NUNC), 4 is one group, and experimental group adds respectively the Semen livistonae chinensis extract that concentration is 0.5 ug/ml, 1.0ug/ ml, 5.0ug/ ml, and matched group then adds isopyknic solvent; Get 1 part of 5% agar solution that melted, join in the 9 parts 37 ℃ fresh RPMI1640 culture fluid that contain 15%FBS, shake up and add in the plate, each 1ml puts room temperature it is solidified; The cell suspension of getting pre-temperature adds the ratio mixing of 5% agar 0.6ml in every 9.4ml, is added in the plate of shop fixtures layer agar, and each 1ml puts 37 ℃, 5%CO ₂Cultivate 7d in the incubator; Contain 50 cell colonies more than the cell at the microscopically counting.

Calculate the medicine cell cluster by following formula and form inhibition percentage

Soft agar clonogenic assay inhibition percentage %=(1-experimental group colony number of cell/cellular control unit colony number) * 100%

2.3 the growth curve method is inoculated in the RPMI1640 culture medium that contains 10%FBS with the Hele7404 cell, NG108-15 then with the DMEM culture medium that contains 10%FBS, is made into respectively 10 * 10 ³Then the cell suspension of individual/ml add in 96 well culture plates, every hole 200 μ l, it is the extract of 0.5 ug/ml, 1.0ug/ ml, 5.0ug/ ml that experimental group adds ultimate density, matched group adds isoconcentration equal-volume solvent. and every kind of every concentration of cell is established three holes, repeats 4 times, puts 10%CO ₂Cultivate in 37 ℃ of incubators, then by 0d, 1d, 2d, 3d, 4d, 5d, 6d, the 7d 40 μ l that respectively take a sample, add 0.4% trypan blue liquid, 10 μ l, the living cell counting number.Every hole counting 4 times is got average, adds up 3 hole averages again, plots figure according to logarithm value and the time effect of cell concentration value, is cell growth curve.

2.4 statistical procedures is with the analysis of SPSS 10.0 statistical softwares, the mean between two groups relatively adopts the t check.

3. experimental result

The result shows that extract has inhibitory action to human hepatoma cell strain Hele7404.

Colony forming method is observed variable concentrations Semen livistonae chinensis extract to the inhibiting tumour cells effect.The results are shown in Table 2.

Table 1 variable concentrations extract is on the impact (%) of inhibiting tumour cells

Annotate: compare with matched group ^▲P＜0.05, ^{▲ ▲}P＜0.01, ^{▲ ▲ ▲}P＜0.005, ^{▲ ▲ ▲ ▲}P＜0.001

As seen from the figure, after the Semen livistonae chinensis extract acts on tumor cell line P388D1, find relatively by medicine group and matched group that oneself can find out that medicine has obvious inhibition lethal effect to tumor cell when concentration was 1.0 μ g/ ml.When drug level was 5.0 μ g/ ml, oneself showed cell culture to the 3 days cell is had stronger inhibition lethal effect, cell mortality after the 4th day.

4, discuss

This experiment is by adopting experimental technique, and the research extract is at extracorporeal anti-tumor function, and its suppression ratio is along with drug level increases and corresponding increasing.The result of the shown result of cell growth curve method result and mtt assay and colony forming method matches.The prompting extract has an antitumor cell effect external.

Mtt assay, colony forming method, growth curve method and Hoechst 33258 fluorescent staining methods are the common methods of observing anti tumor activity in vitro, especially mtt assay is fast and convenient, the external Primary Screening Test of Chang Zuowei antineoplastic agent, the present invention adopts the use in conjunction of several experimental techniques to increase the reliability of experimental result.

Malignant tumor be there is no very good medicine at present.In recent years, oneself becomes the main development of new drug research to seek new antitumor drug from natural plants

Two, inquire into Chinese medicine preparation of the present invention to the inhibitory action of hepatocarcinoma.

The inventor tests discovery, and the preparation made from the Radix Astragali, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, Radix Salviae Miltiorrhizae, Fructus Lycii, the Radix Paeoniae Alba, Radix Ophiopogonis, Carapax et Plastrum Testudinis, Carapax Trionycis, Pericarpium Citri Reticulatae and Radix Glycyrrhizae Preparata can have stronger inhibitory action to mice live body ascites H22 hepatoma carcinoma cell.

1 materials and methods

1.1 Kunming mouse, in 6 ages in week, 21, male BALB/C mice is divided into 3 groups at random, and 7 every group, body weight 18～22 g are purchased from Guangxi Medical University's Experimental Animal Center.

1.2 cell culture

Ascitic type H22 hepatoma cell strain is provided by Colleges Of Traditional Chinese Medicine Of Guangxi, is incubated in RPMI 1640 (GIBCO company) culture fluid that contains 10% hyclone, places C0 ₂Concentration is 5%, relative humidity is 95%, temperature is to cultivate in 37 ℃ the cell culture incubator.The attached cell of cultivating 48,96 h behind the passage is the exponential phase cell, gets the rear 48 h cells that go down to posterity.

1.3 medicine, the medicine of (A group) above-described embodiment 5.(B group) adds water 1000 grams and boiled 2 hours with Radix Astragali Radix Codonopsis aqueous extract, each 100 gram of Radix Astragali Radix Codonopsis; (C group) 1% ethanol,

1.4 test method, every Mouse Weight before the record inoculation.Inoculation exponential phase H22 cell is subcutaneous in the right side of mice groin, inoculum concentration about 6.0 * 106/only.

From inoculation the 6th day, (C group) matched group 5% G/NS solution 0.2～0.5 mL intratumor injection D6,8,10,12.The medicine of embodiment 5 (A group) 1 g/mL 0.2～0.5 mL intratumor injection D6,8,10,12; , (B group) uses Radix Astragali Radix Codonopsis aqueous extract 1 g/mL 0.2～0.5 mL intratumor injection D6,8,10,12; Every range estimation amount right side of mice groin subcutaneous transplantation tumor size from inoculating the 4th.Tumor volume V/4 * a * b ²(a is major diameter, and b is minor axis).Inoculate the 12nd, take off the neck marrow after mice is weighed and put to death tumor-bearing mice, peel off that to take by weighing tumor behind the tumor body heavy.Tumour inhibiting rate=(matched group tumor weigh an administration group tumor heavy)/matched group tumor heavy * 100%: coefficient of concordance=matched group tumor weight * drug combination group tumor is heavy/(single medicine group 1. tumor weight * single medicine group 2. tumor weight); Work as tumour inhibiting rate〉40%, P＜0.05 is determined with antitumor action; When coefficient of concordance＜l for concertedness is arranged.

1.5 statistical method

All data are all used Expression adopts Microsoft excel software that data are done statistical procedures, adopts variance analysis.

2 results (seeing Table 1)

Table 1 Chinese medicine preparation of the present invention is on the impact (C group, blank) of H22 transplanted tumor

Three, discuss

Also discovery of inventor's experiment,

1, the preparation made from the Radix Astragali of the present invention, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, Radix Salviae Miltiorrhizae, Fructus Lycii, the Radix Paeoniae Alba, Radix Ophiopogonis, Carapax et Plastrum Testudinis, Carapax Trionycis, Pericarpium Citri Reticulatae and Radix Glycyrrhizae Preparata also can make mice live body ascites H22 hepatoma carcinoma cell necrosis or the apoptosis phenomenons such as gross distortion, after birth are imperfect, foaming, karyopyknosis occur.

2, test is also pointed out and is had the effect of blood circulation promoting and blood stasis dispelling, hard masses softening and resolving, heat-clearing and toxic substances removing, the body resistance strengthening and constitution consolidating with the Radix Astragali of the present invention, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, Radix Salviae Miltiorrhizae, Fructus Lycii, the Radix Paeoniae Alba, Radix Ophiopogonis, Carapax et Plastrum Testudinis, Carapax Trionycis, Pericarpium Citri Reticulatae and Radix Glycyrrhizae Preparata extract, not only treatment early metaphase hepatocarcinoma there is fine effect and is that function by the enhancing body cell immune system realizes to antitumor action, not only help killing off tumor cells, also help the side effect in the ameliorate tumor chemotherapy.

 

The treatment of cancer example:

1, Lu * *, man, life in 1945, the Nanning people finds abdominal pain, all day belly distending pain, the soreness of waist checks that liver has lump, and biopsy is hepatocarcinoma, do hepatectomy in certain tumour hospital at the beginning of 2009, cut 1/4 liver, after 5 months, again find abdominal pain, hospital required patient to do chemotherapy at that time, patient disagrees with, after change the granule of taking medicine embodiment three of the present invention, twice of every day, each 10 grams, take belly distending pain after 30 days, soreness of waist symptom takes a turn for the better, take one month after, belly distending pain improving situation was survived 1 year.

2, open * *, the woman, nineteen fifty-five gives birth to, the Guangxi Qinzhou schoolman, suffer from hepatocarcinoma in November, 2010, at Qiezhou workers' hospital's hospitalization, check and shift, the abdominal part distending pain is not operated on and is performed an operation, and adopts conservative treatment, family members require to take the Chinese medicinal tablet of the embodiment of the invention two, every day 3-4 time, totally 20 grams, take the aftersensation in January and get that sb.'s illness took a favorable turn, abdominal part distending pain symptom takes a turn for the better, and can arrive outdoor activity, continued to take 3 months, but take a walk to the outside every day, work can do housework.

3, poplar * *, nineteen sixty-eight gives birth to, the Liuzhou city, Guangxi province people, on August 10th, 2009 was suffered from pulmonary carcinoma, right lung pain, dyspnea at examination in hospital, be not in hospital owing to lack of capital, take the capsule of the embodiment of the invention one, every day 3-4 time, totally 15 grams, take the aftersensation in January and get that sb.'s illness took a favorable turn, breathe smooth and easy, present right lung pain, dyspneic symptom takes a turn for the better.

Medicine of the present invention has preferably therapeutic effect to hepatoma Metastasis, and a tree name inventor preliminary test is invigorating the spleen and replenishing QI, the spleen invigorating lung benefiting, and YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire, stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, the effect of the sharp spleen of the liver benefiting.

Claims (3)

1. One kind suppress hepatoma Metastasis Chinese medicine preparation, it is characterized in that: its main effective ingredient is to be made by the raw material of Chinese medicine of following parts by weight:

   Radix Astragali 10-30;

   Radix Codonopsis 10-20;

   Rhizoma Atractylodis Macrocephalae 5-15;

   Herba Hedyotidis Diffusae 20-40;

   Radix Salviae Miltiorrhizae 5-15;

   Fructus Lycii 10-20;

   Radix Paeoniae Alba 5-15;

   Radix Ophiopogonis 5-15;

   Carapax et Plastrum Testudinis 10-25;

   Carapax Trionycis 10-25;

   Pericarpium Citri Reticulatae 5-15;

   Radix Glycyrrhizae Preparata 5-15.
2. 2. the preparation method of the Chinese medicine preparation of inhibition hepatoma Metastasis as claimed in claim 1 is characterized in that: gets Radix Codonopsis and is crushed into powder, and for subsequent use; With Carapax et Plastrum Testudinis, Carapax Trionycis places the copper pot, adds water logging and does not have face, heats with slow fire, keep little and boiled 5-12 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, until abundant fried glue juice is degree, then filter clarification, merge glue that each time fried in shallow oil, remove by filter precipitated impurities, the concentrated glue of receiving, glue juice after filtering is put in the copper pot concentrated with the slow fire heating, constantly stirred, in case coking, to be concentrated to " extension flag " the rear glue of receiving, in glue impouring gel dish, make it natural condensation, solidify for accelerating glue juice, also can add the 5%-10% Colla Corii Asini, good blob of viscose is solidified in taking-up, cuts into pieces with cutting the glue cutter, and is for subsequent use; The Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, Radix Salviae Miltiorrhizae, Fructus Lycii, the Radix Paeoniae Alba, Radix Ophiopogonis, Pericarpium Citri Reticulatae and Radix Glycyrrhizae Preparata volumetric concentration are the ethanol extraction twice of 50-90%, and merge extractive liquid, is for subsequent use; Concentrated after filtering, Recycled ethanol then adds Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, and concentrated, gets dry extract, merges with above-mentioned Radix Codonopsis powder again, and mix homogeneously adds filler, makes capsule or granule, tablet, obtains product; Perhaps add sucrose or Mel, mix homogeneously is made pill.
3. 3. the application of the Chinese medicine preparation of inhibition hepatoma Metastasis claimed in claim 1 aspect the medicine of preparation Hepatoma therapy.