CN102114099B - Traditional Chinese medicine preparation for inhibiting liver cancer and preparation method thereof - Google Patents

Abstract

The invention relates to a traditional Chinese medicine preparation for inhibiting liver cancer and the preparation method thereof. The traditional Chinese medicine preparation is prepared from ginseng, milkvetch root, largehead atractylodes rhizome, tortoise plastron, oldenlandia, turtle carapace, white peony root, prepared root of common monkshood, eucommia bark and processed licorice root. The preparation method comprises the following steps: separately grinding ginseng and processed licorice root into powder, extracting with water or ethanol, concentrating, mixing, adding bulking agent, and making into solid preparation or liquid preparation products. The traditional Chinese medicine preparation can be used for treating both principal and secondary aspect of disease, and has the effects of relieving pain, dispersing accumulation of pathogen, nourishing yin, nourishing blood, invigorating qi, regulating human immune system functions, resisting cancer, keeping toxin expelling passages smooth, and inhibiting growth of cancer cells.

Description

Suppress Chinese medicine preparation of hepatocarcinoma and preparation method thereof

Technical field

The present invention relates to a kind of Chinese medicine preparation that suppresses tumor, particularly suppress Chinese medicine preparation of hepatocarcinoma and preparation method thereof.

Background technology

Primary hepatocarcinoma is the 6th modal cancer and the 3rd the modal cancer cause of the death in the world.The primary hepatocarcinoma M & M of developing country is higher, and primary hepatocarcinoma that China takes place has just accounted for the over half of the total case of hepatocarcinoma in the world.Think that at present the drinking water of hepatitis B virus, food pollution AFB1, pollution and heavy drinking etc. are closely related with the generation of primary hepatocarcinoma.In today of industrial civilization prosperity; People are constantly in the face of various ambient pressures, environmental pollution; Factor affecting such as water, air and chemical fertilizer, pesticide, vehicle exhaust, food additive, unhygienic diet and often edible are pickled food, the smoking of fire-cureing, drunk, noise jamming and atmosphere radioactive substance.These various heterotoxins get into people's body, health risk through different approaches; And the disorder of body metabolic function; Pile up the various refuses that produce in the metabolism extremely easily and be stuck in the metabolism pipeline; Stop up normal drain passage, constitute the endogenous toxin, bring out human body and produce various condition of illness.Finally can cause the various dysfunctions of body, like the endocrine disturbance, degradation under lipid metabolism imbalance, the function of immune system causes the generation of tumor.

Because hepatocarcinoma is one type of disease of serious threat human health, almost seldom can cure.Therefore, hepatocarcinoma is the emphasis of medical research always, seeks effective antitumour medicine and method, and thoroughly capturing hepatocarcinoma is the important subject of world medical circle.Research shows that many Chinese medicines have antihepatocarcinoma effect, and especially compound recipe has multiple action component and produces antitumor action through multiple action pathway.Some medical facility and medical personnel have delivered the more open source literature report that utilizes Chinese herbal treatment hepatopathy or hepatocarcinoma in recent years, for example:

1, Chinese patent; Title: a kind of anticancer dispersing tumor toxin expelling soup application (patent) number: the CN200410038090.X applying date: 2004.05.19; Open (bulletin) number: CN1698684 open (bulletin) day: 2005.11.23 applies for (patent right) people: the big address of clock: (design) people invents in No. 89 Guangzhous, Linhe West Road, Guangzhou City, Guangdong Province out-patient department of Friendship Hospital: the big summary of clock: a kind of anticancer dispersing tumor toxin expelling soup; Employing has the Chinese medicine of heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, anticancer dispersing tumor effect; Be principal agent wherein, be aided with the Radix Astragali, Radix Ginseng, Ganoderma, Radix Notoginseng, the Rhizoma Atractylodis Macrocephalae, triangular, Rhizoma Curcumae, Semen Persicae, Flos Carthami, Herba Hedyotidis Diffusae etc. Chinese herbal medicine is fried and form with animal drugs such as Bufo siccus, ground beetle, Hirudo, Mylabris, Gekko Swinhonis.The present invention both can directly kill tumor cell; The destruction focus combines, but antiviral, antiinflammatory, destruction tumor vessel prevent cell transfer again, and anti-cell suddenlys change, improves the cell means of information transmission; The drug resistance of reversing tumor cell; While human body immunity improving function, thus various innocent and malignant tumours effectively treated, especially treat hepatocarcinoma, pulmonary carcinoma, breast carcinoma best results.Principal claim: 1. anticancer dispersing tumor toxin expelling soup; Employing has the Chinese medicine of heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, anticancer dispersing tumor effect and fries in shallow oil; It is characterized in that: it is principal agent with the animal drugs; Be aided with other Chinese herbal medicine and fry in shallow oil, the concrete consumption of each medicine is Bufo siccus 5g, ground beetle 10g, Hirudo 2g, Mylabris 0.04g, Gekko Swinhonis 6g, Radix Astragali 30g, Radix Ginseng 10g, Ganoderma 15g, Radix Notoginseng 10g, Rhizoma Atractylodis Macrocephalae 20g, triangular 8g, Rhizoma Curcumae 8g, Semen Persicae 10g, Flos Carthami 6g, Herba Hedyotidis Diffusae 30g.

2, Chinese patent; Title: a kind of Chinese medicine medicine for preventing powder and preparation method thereof application (patent) number: the CN02116298.0 applying date: 2002.04.01 open (bulletin) number: CN1448164 open (bulletin) day: 2003.10.15 apply for (patent right) people: Yang Wen show address: Qiqihaer City, Heilongjiang Province Tiefeng District railway north office invents (design) people: Yang Wenxiu for 3-12 number and makes a summary the 18-2 building, road of weaving in residence north: a kind of Chinese medicine medicine for preventing powder and preparation method thereof; The prescription I that its Chinese powder medicine is used by taking medicine before meal and the prescription II of one after each meal form, and the component of its prescription I has the pure Chinese medicine of 21 flavors such as Olibanum, Myrrha, Radix Angelicae Sinensis, Pericarpium Citri Reticulatae, Radix Et Rhizoma Rhei, Natrii Sulfas, Radix Paeoniae Rubra, Fructus Gleditsia, eupolyphage sece steleophaga, Squama Manis.The component of its prescription II has the pure Chinese medicine of 13 flavors such as Radix Ginseng, the Rhizoma Atractylodis Macrocephalae, Poria, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Angelicae Pubescentis, Rhizoma Et Radix Notopterygii, the Radix Astragali, Radix Aconiti Lateralis Preparata.Its preparation method is: the required pure Chinese medicine of prescription I is carried out selected (not containing Natrii Sulfas) respectively with after the clear water rinsing by prescription; Under 100 ℃ condition, dry, through ultraviolet disinfection 30 minutes, the cooling back was broken into 120 order fine powders by the formula proportion mixed powder; Again the Natrii Sulfas component is developed into 120 order fine powders; After the fine powder mixing and stirring that crushes with above-mentioned prescription I medicine, incapsulate (0.5g/ grain), reinstall in the bottle and seal.Prescription II does not have Natrii Sulfas, and its preparation method and prescription I are similar.This medicine has human body immunity improving power, promotes metabolism, suppresses or the dissolved cancer cell function, and multiple cancers such as hepatocarcinoma are had better curative effect, and taking convenience has no side effect.Principal claim: 1. Chinese medicine medicine for preventing powder is characterized in that: the prescription I that it is used by taking medicine before meal and the prescription II of one after each meal form, its set of dispense than (weight portion) as follows: prescription I (taking medicine before meal with): Olibanum 2.5-3%, Myrrha 2.5-3%; Radix Angelicae Sinensis 1.8-2% Pericarpium Citri Reticulatae 2.5-3%, Radix Et Rhizoma Rhei 6.4-6.8%, Natrii Sulfas 4.5-5% Radix Paeoniae Rubra 4.5-5%, Fructus Gleditsia 4.5-5%; Eupolyphage sece steleophaga 5.4-5.8% Squama Manis 8-10%, Radix Angelicae Dahuricae 0.8-1%, pollen 2.5-3% Stigma Croci 4.5-5%; Flos Lonicerae 8-10%, the windproof 1.8-2% of Radix Aucklandiae 8-10%, Pseudobulbus Bletillae (Rhizoma Bletillae) 8-10%; The Radix Bupleuri 1.3-1.5% core 4.5-5% that withers, the skin 4.5-5% that withers, Radix Notoginseng 4.5-5%; Prescription II (one after each meal): Radix Ginseng 8-9%, Rhizoma Atractylodis Macrocephalae 11-12%, Poria 8-9% Radix Glycyrrhizae 3-4%, Radix Angelicae Sinensis 3-4%; Radix Angelicae Pubescentis 4-4.5%, Rhizoma Et Radix Notopterygii 3-4%, Radix Astragali 17-18%, Radix Paeoniae Alba 11-12%; Radix Aconiti Lateralis Preparata 2.5-3%, Ramulus Cinnamomi 11-12%, crust halberd 5-6%, Semen Coicis 8-9%.

3, Chinese patent; Title: a kind of Chinese medicine composition of treating the liver cirrhosis disease and preparation method thereof; Application (patent) number: the CN200910309349.2 applying date: 2009.11.05 open (bulletin) number: CN101869686A; Open (bulletin) day: 2010.10.27 applies for (patent right) people: Thailand one and Pu (Beijing) Chinese medicine academy company limited; The address: invention (design) people: Wang Feng in 2027b, Building 9, No. 16, Fengguan Road, Fengtai District, Beijing City makes a summary: a kind of Chinese medicine composition of treating the liver cirrhosis disease and preparation method thereof, Chinese medicine composition mainly are made up of following crude drug: Sanguis Draxonis, Squama Manis, Rhizoma Chuanxiong, Radix Paeoniae Rubra, the Radix Paeoniae Alba, Poria, the Rhizoma Atractylodis Macrocephalae, Semen Persicae, Flos Carthami, Ganoderma, Sanguis Draxonis, Rhizoma Corydalis, Radix Angelicae Sinensis, triangular, Rhizoma Curcumae, Pericarpium Citri Reticulatae, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Ginseng, Radix Astragali, Radix Rehmanniae Preparata, Radix Puerariae, Colla Plastri Testudinis, Cornu Cervi Degelatinatum, Colla carapax Trionycis, Radix Rehmanniae, Radix Scrophulariae, Fructus Corni, the Radix Linderae, Fructus Aurantii, Rhizoma Cyperi, Pericarpium Citri Reticulatae, the Radix Aucklandiae, Lignum Aquilariae Resinatum, Cortex Magnoliae Officinalis, Fructus Evodiae, Bulbus Allii Macrostemonis, Aspongopus.Chinese medicine composition of the present invention can be prepared into any oral formulations commonly used according to the method for Chinese medicinal of routine.It is main that the present invention can significantly improve lassitude and weak, loss of appetite and dyspepsia, can have nauseating, to detest oil, abdominal part flatulence, epigastric discomfort, dull pain and diarrhoea, ordinary circumstance and nutriture relatively poor, it is weak to become thin; Lassitude; Serious symptom person is weak and be unable to leave the bed, and follows xerosis cutis coarse, and complexion is gloomy pitch-dark; Symptoms such as anemia, glossitis, angular cheilitis, nyctalopia, polyneuritis and edema and water and electrolyte disturbance, portal hypertension; Clinical efficacy is definite, and is evident in efficacy, takes effect rapidly.Because Chinese medicine composition of the present invention has adopted the integration of edible and medicinal herbs medicine of NF regulation to make up basically, so have cost, advantage such as have no side effect basically.

Summary of the invention

For reaching this purpose, the inventor studies Traditional Chinese medical theory, on the basis of secret prescription handed down in the family from generation to generation, replenishes and puts in order, excavates and develop a kind of adjusting viscera function imbalance, suppresses Chinese medicine preparation of hepatocarcinoma and preparation method thereof.

The present invention suppresses the effective ingredient of the Chinese medicine preparation of hepatocarcinoma and is processed by following parts by weight of traditional Chinese medicines raw material:

Radix Ginseng 8-15 Radix Astragali 3-10 Rhizoma Atractylodis Macrocephalae 3-10 Carapax et Plastrum Testudinis 10-20 Herba Hedyotidis Diffusae 15-30

Carapax Trionycis 3-10 Radix Paeoniae Alba 8-15 Radix Aconiti Lateralis Preparata 3-10 Cortex Eucommiae 3-10 Radix Glycyrrhizae Preparata 3-10.

According to Chinese medicine document record, more than the side of said each composition of Chinese medicine separate and be:

Radix Ginseng, Latin plant name: Panax ginseng C.A.Mey, sweet, the little hardship of property, tepor, strongly invigorating primordial QI, invigorating the spleen to benefit the lung; According to bibliographical information, the multiple Saponin, ginseng polysaccharide and the ginseng essential oil that contain in the Radix Ginseng have antitumor action.The ginsenoside can make cancerous cell break up, induce and reverse and be non-cancerous cell.Stem and leaf of Radix Ginseng total saponins can suppress the speed of growth and the splitting ability of In vitro culture gastric carcinoma cells, increases the former content of endocellular sugar, reduces mucopolysaccharide and activity of acid phosphatase in the cell, plays the certain obstruction stomach cancer cell growth and the effect of propagation.Flos Ginseng, leaf glycol group Saponin also have certain antitumor action external.Radix Ginseng aerial parts volatile oil composition can make the gastric cancer of In vitro culture and HCC number reduce, growth is suppressed, dead cancerous cell number increases.

The Radix Astragali, latin name: Leguminosae invigorating QI to consolidate the body surface resistance, diuresis poison holding, QI invigorating invigorating middle warmer.The Radix Astragali is a leguminous plant, and it is a Chinese medicine commonly used simply.Its main pharmacological is " benefiting QI for strengthening the superficies ", can " diuretic ", and also can " promoting pus discharge and tissue regeneration strengthening ".

The Radix Astragali and Radix Ginseng all belong to QI invigorating good medicine, but Radix Ginseng biases toward strongly invigorating primordial QI, and recuperating depleted YANG and rescuing the patient from collapse is usually used in emergency cases such as collapse, shock, and effect is better.The Radix Astragali is main with tonify deficiency then, be usually used in asthenia with the passing of time, low, the thready pulse without strength person of speech.Radix Astragali tool and mending and oiliness characteristics, and if Radix Ginseng, tonic compatibilities such as Radix Codonopsis are better effects if then.But Radix Astragali single uses, and also can use with the other medicines compatibility.

The Rhizoma Atractylodis Macrocephalae, latin name: Rhizoma Atractylodis Macrocephalae, function cures mainly: invigorating the spleen and benefiting QI, the dampness diuretic, hidroschesis, antiabortive.Be used for insufficiency of the spleen lack of appetite, abdominal distention is had loose bowels, phlegm retention vertigo and palpitation, edema, spontaneous perspiration, frequent fetal movement.It is reported that mouse stomach Rhizoma Atractylodis Macrocephalae decocting liquid can be prevented and treated the hepatic injury due to the carbon tetrachloride, alleviate hepatic glycogen minimizing and hepatocellular degeneration necrosis, promote hepatocyte to increase, the ALT of rising is descended.

Carapax et Plastrum Testudinis is sternite and the carapace of Testudinidae animal chinemys reevesii.Function is the nourishing kidney YANG hyperactivity suppressing, replenishing kidney and strengthening bone, the reinforcing the heart of nourishing blood.Cure mainly: 1. hyperactivity of YANG due to deficiency of YIN, interior-heat caused by deficiency of YIN, stirring-up of pathogenic wind in the interior resulting from deficiency.2. the atrophic debility of bones of suffering from a deficiency of the kidney, infantile metopism.3. deficiency of YIN-blood is palpitated with fear, and insomnia is forgetful.Effect: to the effect of immunologic function, the experiment proof: Testudinis deer oral liquor for spleen and kidney and pill give mice oral, can improve the phagocytic function of its reticuloendothelial system, significantly increase phagocytic index and engulf coefficient; The spleen that makes mice under normal and the immunosuppressive condition, the effect of thymus weightening finish are also arranged; The tip leukopenia that cyclophosphamide is caused has the certain protection effect. bibliographical information; Carapax et Plastrum Testudinis has antitumor action can improve body antineoplastic immunocompetence, and its extract has inhibitory action to sarcoma S180, ehrlich ascites tumor and ascitic type liver cancer.

Herba Hedyotidis Diffusae (formal name used at school: Hedyotis diffusa) be the plant of Rubiaceae cerastium.Its Chinese patent medicine bitter in the mouth, light, cold in nature.Main effect is heat-clearing and toxic substances removing, medicinal powder for relieving pain knot, promoting urination to remove dampness.Outstanding kind treatment all kinds inflammation has than strong inhibitory action the tumor cell of acute lymphoblastic type, granulocyte type, monocyte type and chronic granulocyte type at external (quite crude drug 6g/ml).

Carapax Trionycis Carapax Amydae is the carapace of Trionychidae animal Trionyx sinensis Wiegmann Trionyx sinensis Wiegmann.Meridian distribution of property and flavor: salty, be slightly cold.Return liver, kidney channel.Function cures mainly: nourishing YIN for suppressing the hyperactive YANG, hard masses softening and resolving is brought down a fever to remove and is steamed.Be used for fever due to yin deficiency, consumptive fever hectic fever due to YIN-deficiency, stirring-up of pathogenic wind in the interior resulting from deficiency, amenorrhea ， WEIJIA, chronic malaria malaria with splenomegaly.Have report to point out, former powder of Trionyx sinensis Wiegmann and Turtle Shell have inhibitory action to transplanting solid carcinoma, and the tumor diameter is significantly reduced, and tumor weight significantly alleviates; But ehrlich ascites carcinoma then there is not remarkable effect. also there is report to point out; In order to Carapax Trionycis is the aqueous solution supernatant of master's the diffusing pill of herbal mixture Carapax Trionycis; After quantitatively inoculating for cancerous cell; Observe its effect situation to growth of cancer cells, the result shows: the diffusing supernatant of Carapax Trionycis all has inhibitory action to mouse lymphocyte leukemia (L1210), HL-60 and the growth of gastric cancer 803 cells.

The Radix Paeoniae Alba; (formal name used at school: Paeonia sterniana Fletcher in Journ.) also claiming Paeonia sterniana Fletcher in Journ., is Ranunculaceae Paeonia plant, pharmacological action: 1, to the effect of cardiovascular system: the Radix Paeoniae Alba has coronary artery dilator to Radix Paeoniae Alba; The effect of bringing high blood pressure down (d-cachou extract and progallin A have antithrombotic and antiplatelet aggregative activity); 2, liver protection effect: the Radix Paeoniae Alba has obvious protective effect to hepatic injury due to carbon tetrachloride, AFB1, the D-galactosamine, and 3, spasmolysis: the Radix Paeoniae Alba has inhibitory action to intestinal tube and stomach on the throne motion, can significantly resist the uterine contraction that oxytocin causes; 4, analgesic activity: the Radix Paeoniae Alba can suppress mouse writhing, shouts, the hot plate reaction; Morphine is suppressed writhing response has synergism, and can resist and faint from fear due to the pentylenetetrazole, and 5, antibacterial action.The Radix Paeoniae Alba has protective effect to liver; Radix Paeoniae Alba extract raises to hepatic injury due to the D-galactosamine and SGPT has tangible antagonism; SGPT is reduced; And make hepatocellular pathological changes recover normal with downright bad. the lactic acid dehydrogenase that the rat acute hepatic injury that the ethanol extraction of the Radix Paeoniae Alba can make aflatoxin B1 cause is showed and the gross activity of isozyme raise and are minimized. and white peony root's total glycoside can suppress carbon tetrachloride induced mice Plasma glutamate-pyruvate transaminase and lactic acid dehydrogenase raises; And to liver organization bite acid denaturation, necrosis has certain antagonism. the someone thinks, the hepatic injury due to the carbon tetrachloride is to destroy because of membrane structure generation peroxidation, makes that glutamate pyruvate transaminase and lactic acid dehydrogenase raise in the blood; White peony root's total glycoside possibly have protective effect to hepatocyte injury, therefore the transaminase is reduced.

Radix Aconiti Lateralis Preparata, Radix Aconiti Lateralis Preparata are the processed goods of the daughter root of ranunculaceae plant Aconitum carmichjaelii Debx. aconitum carmichaeli debx..Meridian distribution of property and flavor: hot, sweet, big heat; Poisonous.GUIXIN, kidney, spleen channel.Function cures mainly: recuperating depleted YANG and rescuing the patient from collapse, it is supporing yang to mend fire, dispersing cold for relieving pain." for the recuperating depleted YANG and rescuing the patient from collapse first article medicine ", Radix Aconiti Lateralis Preparata has recuperating depleted YANG and rescuing the patient from collapse, and it is supporing yang to mend fire, by the effect of wind-cold damp pathogen.Cure mainly the YANG-depletion collapse, the cold extremities faint pulse, sexual impotence, cold womb, trusted subordinate's cold type of pain, deficiency and coldness is vomited and diarrhoea, YIN-cold edema, arthralgia due to cold-dampnes etc." book on Chinese herbal medicine converges and says " cloud: " YIN-cold looses for Radix Aconiti Lateralis Preparata, recuperating depleted YANG gas.The time of all genus yang deficiency negative electrodes, the heat syndrome person of lung kidney unit, clothes dead distinguished service arranged ".Radix Aconiti Lateralis Preparata can strengthen myocardial contraction, accelerates heart rate, increases cardiac output, increases myocardial oxygen consumption.Adopt Chinese medicine serum pharmacological research method; After observing oral Radix Aconiti Lateralis Preparata crude preparation by using, animal serum has obvious enhancing myocardial contraction and the effect of accelerating myocardial contraction speed, administration after 2 hours the effect of pastille serum reach the peak; Show and really close the heart tonifying composition in the Radix Aconiti Lateralis Preparata decocting liquid, and this composition is oral effectively.The Radix Aconiti Lateralis Preparata over administration can cause arrhythmia.

The Cortex Eucommiae is the dry bark of the Eucommiaceae plant Cortex Eucommiae (Eucommia ulmoides Oliver), [nature and flavor] Gan Weixin, temperature.1. " herbal classic ": " acrid in the mouth is flat." 2. " not Lu ": " be sweet, and temperature is nontoxic." 3. " property of medicine opinion ": " bitter in the mouth." 4. hot, flat, nontoxic, regulate the flow of vital energy and enrich blood.5. Pharmacopoeia of the People's Republic of China version in 2010 is one one: " sweet, temperature.Return liver, kidney channel." extract and the decoct of eucommia bark have persistent hypotensive effect to animal.The modern pharmacology experiment proof Cortex Eucommiae has anticancer and the effect that presses down cancer, and its effective ingredient is relevant with its contained lignanoid, phenylpropyl alcohol element and iridoid.According to report, the contained Geniposidic acid methyl ester of the Cortex Eucommiae has antineoplastic action.The contained syringaresinol disaccharidase glycosides of the Cortex Eucommiae has active preferably in Lymphocytic leukemia P388 (Ps) system, concentration 12.5mg/kg may command T/C value) >=126.Okada etc. are general isolatedly flavone from the Folium Eucommiae chloroform extract, is a kind of immunosuppressive substance of the T of interference lymphocyte function, and human nasopharyngeal carcinoma (KB) and mouse lymphotactin leukemia (P388) are all had GIA.

Radix Glycyrrhizae Preparata (RADIX GLYCYRRHIZAE PREPARATA) is a leguminous plant, and Radix Glycyrrhizae Preparata is the Radix Glycyrrhizae of smoking with honey.Function with cure mainly: spleen reinforcing stomach function regulating, Yiqi and vein recovery.Be used for weakness of the spleen and stomach, fatigue and weakness, severe palpitation, irregularly intermittent and regularly intermittent pulse can be separated the Radix Aconiti Lateralis Preparata poison.Main effect: nourishing YIN, enrich blood, negative and positive and transferring.

More than all medicines share reinforcement and elimination in combination, medicinal powder for relieving pain knot; Giving consideration to both the incidental and fundamental; Play nourishing YIN and benefiting blood, Yiqi and vein recovery, promoting blood circulation and stopping pain and adjusting digestive system and human immune system's function altogether, to resisting tumor, keeping the toxin expelling pipeline unimpeded, the growth of anticancer all has effect.

Again from the another one angle; Chinese medicine is that China's tradition is used for the important weapon of disease preventing and treating; In recent years, people found and developed some medicines that the treatment cancer is arranged from Chinese medicine, like camptothecine, maytansine, paclitaxel etc.; And Chinese medicine ingredients and the mechanism of action thereof with anti-tumor activity carried out more deep research, existing Chinese medicine antitumor action mechanism summary just of the present invention as follows:

The Chinese herbal treatment malignant tumor; The determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs of foot wholistic therapy phase, just whole and part combines, has both seen the defensive ability/resistance ability of body to pathogenic factor, sees the influence of local patholoic change to body again; On differential diagnosis of diseases and dialectical basis; Through the composition of prescription of some specific drugs with strong points, get into body rightly, regulate the balance of QI and blood, negative and positive, body fluid.Drug molecule gets into whole body through physical reactions and chemical reaction, and killing cancer cell is enclosed in the surface that is adsorbed on cell that has, corrosion, and this can be called " battle of encirclement "; The medicament composing prescription that has through different effects, is cut apart cancerous cell, defeats in detail again, and this can be called " fighting war "; The effect of passing through medicine itself that has attaches with synergistic medicine, can tumoricidal blood circulation, and remove and change and love the partial blood fortune of body, the propagation of control cancer cell, this can be called " instigating rebellion within enemy camp war "; The combination body condition that has, through medicine transfer, the function of enhancing, human body immunity improving cell removes to engulf cancerous cell, this can be called " immunity war "; The nature and flavor that pass through medicine that have add and use local application, go to destroy the structure and the generation of collapsing cell with temperature, and this can be called " jamming war "; The medicine that has can be regulated QI and blood, can make QI-blood circulation normal, isolates cancerous cell, makes it to excrete, and this can be called " expulsion war "; What have can influence the cancerous cell endoenzyme: making it becomes " end fat ", makes cancerous cell chromosome, gene necrosis, and this can be called " sabotage operations "; Can help immunocyte chromosome, the gene that have restore, and this can be called " drawing war over to one's side "; What have can make QI and blood energy mean allocation. hungry cancerous cell, make it to revert to normal cell, and this can be called " balance war ".

So Chinese medicine preparation of the present invention has been followed whole existing the thought and the determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs principle of motherland's medical science, from the rule of molecular level research and inquirement malignant tumor incidence and development. and adopt blood circulation promoting and blood stasis dispelling, hard masses softening and resolving, heat-clearing and toxic substances removing, the body resistance strengthening and constitution consolidating etc. to have " operation " means of Chinese medicine characteristic; Accomplish " medication is as using military forces ", ability " To know one's own strength and the enemy's is the sure way to victory "; In the assault of fortified positions of going on an expedition cancer; Handy, set up outstanding service, make Chinese medicine demonstrate immeasurable huge effect.

The Chinese medicine preparation that the present invention suppresses hepatocarcinoma can add filler or the solution that meets medical standard; Process medically solid preparation or liquid preparation; For example tablet, capsule, granule, pill, oral liquid or syrup etc., below introduce the method for preparing of several kinds of dosage forms respectively:

Described solid preparation method for preparing is following:

Get Radix Ginseng and be crushed into powder, subsequent use; Carapax et Plastrum Testudinis, Carapax Trionycis are placed the copper pot, add water logging and do not have face, with the slow fire heating, keep little and boiled 8-24 hour, inclining decocting liquid; Successively fry in shallow oil 3-5 time altogether, be degree, filter clarification then, merge glue that each time fried in shallow oil, remove by filter precipitated impurities until abundant fried glue juice; Concentrate to receive glue, the glue juice after filtering is put in the copper pot concentrated, constantly stir, in case coking with the slow fire heating; Wait to be concentrated into " extension flag " back and receive glue, in glue impouring gel dish, make it the nature condensation, solidify for accelerating glue juice; Also can add the 5%-10% Colla Corii Asini, take out and solidify good blob of viscose, cut into pieces with cutting the glue cutter, subsequent use.The Radix Astragali, Herba Hedyotidis Diffusae, the Radix Paeoniae Alba, Radix Aconiti Lateralis Preparata, the Cortex Eucommiae and Radix Glycyrrhizae Preparata use the ethanol extraction twice of volumetric concentration as 50-90%, and merge extractive liquid, is subsequent use; Filtration concentrates behind the recovery ethanol, adds Carapax et Plastrum Testudinis, Colla carapax Trionycis juice then, also concentrates, and gets dry extract, and with above-mentioned Radix Ginseng powder merging, mix homogeneously adds filler, processes capsule or granule, tablet, obtains product again; Perhaps add sucrose or Mel, mix homogeneously is made pill.

The manufacture method of above-described oral liquid is to get Radix Ginseng, Radix Glycyrrhizae Preparata, adds decocting in water 2-4 hour respectively, and is subsequent use; Carapax et Plastrum Testudinis, Carapax Trionycis are placed the copper pot, add water logging and do not have face, with the slow fire heating, keep little and boiled 8-24 hour, inclining decocting liquid; Successively fry in shallow oil 3-5 time altogether, be degree, filter clarification then, merge glue that each time fried in shallow oil, remove by filter precipitated impurities until abundant fried glue juice; Reconcentration is received glue, the glue juice after filtering is put in the copper pot concentrate with the slow fire heating, constantly stirs, in case coking; Wait to be concentrated into " extension flag " back and receive glue, in glue impouring gel dish, make it the nature condensation, solidify for accelerating glue juice; Also can add people 5%-10% Colla Corii Asini, take out and solidify good blob of viscose, cut into pieces with cutting the glue cutter, subsequent use.The Radix Astragali, Herba Hedyotidis Diffusae, the Radix Paeoniae Alba, Radix Aconiti Lateralis Preparata and the Cortex Eucommiae are used the ethanol extraction twice of volumetric concentration as 50-90%, and merge extractive liquid, is subsequent use; Ethanol is reclaimed in the filtration back, concentrates, and then, adds Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, also concentrates; Get dry extract, merge with above-mentioned Radix Ginseng, Radix Glycyrrhizae Preparata solution again, add the proper amount of edible antiseptic; With the pure water that adds the total drug weight 3-5 of dry extract times, process oral liquid, obtain product.

The specific embodiment

Following embodiments of the invention:

Embodiment one:

Get 8 kilograms of Radix Ginsengs, be crushed into powder, subsequent use; 10 kilograms of Carapax et Plastrum Testudiniss, 10 kilograms of Carapax Trionycis are placed the copper pot, add water logging and do not have face, with the slow fire heating, keep little and boiled 8 hours, inclining decocting liquid; Successively fry in shallow oil 3-5 time altogether, be degree, filter clarification then, merge glue that each time fried in shallow oil, remove by filter precipitated impurities until abundant fried glue juice; Concentrate to receive glue, the glue juice after filtering is put in the copper pot concentrated, constantly stir, in case coking with the slow fire heating; Wait to be concentrated into " extension flag " back and receive glue, in glue impouring gel dish, make it the nature condensation, solidify for accelerating glue juice; Also can add people 5%-10% Colla Corii Asini, take out and solidify good blob of viscose, cut into pieces with cutting the glue cutter, subsequent use.3 kilograms of the Radixs Astragali, 3 kilograms of the Rhizoma Atractylodis Macrocephalaes, 15 kilograms of Herba Hedyotidis Diffusaes, 8 kilograms of the Radix Paeoniae Albas, 3 kilograms of Radix Aconiti Lateralis Preparatas, 3 kilograms of the Cortexs Eucommiae, 3 kilograms of Radix Glycyrrhizae Preparatas use the ethanol extraction twice of volumetric concentration as 50-90%, and merge extractive liquid, is subsequent use; Reclaim ethanol after filtering, concentrate, add Carapax et Plastrum Testudinis, Colla carapax Trionycis juice then, also concentrate, get dry extract, merge with above-mentioned Radix Ginseng powder, mix homogeneously adds filler, processes capsule.

Embodiment two:

Get 10 kilograms of Radix Ginsengs, be crushed into powder, subsequent use; With 15 kilograms of Carapax et Plastrum Testudiniss, 8 kilograms of Carapax Trionycis place the copper pot, add water logging and do not have face, heat with slow fire; Keep little and boiled 8-24 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, is degree until abundant fried glue juice, filters clarification then; Merge glue that each time fried in shallow oil, remove by filter precipitated impurities, concentrate receipts glue, the glue juice after filtering is put in the copper pot concentrated constantly stirring with the slow fire heating; In case coking is waited to be concentrated into " extension flag " back and is received glue, in glue impouring gel dish, make it the nature condensation, solidify for accelerating glue juice; Also can add people 5%-10% Colla Corii Asini, take out and solidify good blob of viscose, cut into pieces with cutting the glue cutter, subsequent use.5 kilograms of the Radixs Astragali, 20 kilograms of Herba Hedyotidis Diffusaes, 10 kilograms of the Radix Paeoniae Albas, 5 kilograms of Radix Aconiti Lateralis Preparatas, 5 kilograms of 5 kilograms of the Cortexs Eucommiae and Radix Glycyrrhizae Preparatas use the ethanol extraction twice of volumetric concentration as 50-90%, and merge extractive liquid, is subsequent use; Reclaim ethanol after filtering, concentrate, add Carapax et Plastrum Testudinis, Colla carapax Trionycis juice then, also concentrate, get dry extract, merge with above-mentioned Radix Ginseng powder, mix homogeneously adds filler, processes tablet.

Embodiment three:

Get 8 kilograms of Radix Ginsengs, be crushed into powder, subsequent use; 10 kilograms of Carapax et Plastrum Testudiniss, 10 kilograms of Carapax Trionycis are placed the copper pot, add water logging and do not have face, with the slow fire heating, keep little and boiled 8 hours, inclining decocting liquid; Successively fry in shallow oil 3-5 time altogether, be degree, filter clarification then, merge glue that each time fried in shallow oil, remove by filter precipitated impurities until abundant fried glue juice; Concentrate to receive glue, the glue juice after filtering is put in the copper pot concentrated, constantly stir, in case coking with the slow fire heating; Wait to be concentrated into " extension flag " back and receive glue, in glue impouring gel dish, make it the nature condensation, solidify for accelerating glue juice; Also can add people 5%-10% Colla Corii Asini, take out and solidify good blob of viscose, cut into pieces with cutting the glue cutter, subsequent use.3 kilograms of the Radixs Astragali, 3 kilograms of the Rhizoma Atractylodis Macrocephalaes, 15 kilograms of Herba Hedyotidis Diffusaes, 8 kilograms of the Radix Paeoniae Albas, 3 kilograms of Radix Aconiti Lateralis Preparatas, 3 kilograms of the Cortexs Eucommiae, 3 kilograms of Radix Glycyrrhizae Preparatas use the ethanol extraction twice of volumetric concentration as 50-90%, and merge extractive liquid, is subsequent use; Reclaim ethanol after filtering, concentrate, add Carapax et Plastrum Testudinis, Colla carapax Trionycis juice then, also concentrate, get dry extract, merge with above-mentioned Radix Ginseng powder, mix homogeneously adds filler, processes granule.

Embodiment four:

Get 10 kilograms of Radix Ginsengs, be crushed into powder, subsequent use; With 15 kilograms of Carapax et Plastrum Testudiniss, 8 kilograms of Carapax Trionycis place the copper pot, add water logging and do not have face, heat with slow fire; Keep little and boiled 8-24 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, is degree until abundant fried glue juice, filters clarification then; Merge glue that each time fried in shallow oil, remove by filter precipitated impurities, concentrate receipts glue, the glue juice after filtering is put in the copper pot concentrated constantly stirring with the slow fire heating; In case coking is waited to be concentrated into " extension flag " back and is received glue, in glue impouring gel dish, make it the nature condensation, solidify for accelerating glue juice; Also can add people 5%-10% Colla Corii Asini, take out and solidify good blob of viscose, cut into pieces with cutting the glue cutter, subsequent use.5 kilograms of the Radixs Astragali, 20 kilograms of Herba Hedyotidis Diffusaes, 10 kilograms of the Radix Paeoniae Albas, 5 kilograms of Radix Aconiti Lateralis Preparatas, 5 kilograms of 5 kilograms of the Cortexs Eucommiae and Radix Glycyrrhizae Preparatas use the ethanol extraction twice of volumetric concentration as 50-90%, and merge extractive liquid, is subsequent use; Reclaim ethanol after filtering, concentrate, add Carapax et Plastrum Testudinis, Colla carapax Trionycis juice then, also concentrate, get dry extract, merge with above-mentioned Radix Ginseng powder, mix homogeneously adds filler, processes pill.

Embodiment five:

Get 18 kilograms of Carapax et Plastrum Testudiniss, 10 kilograms of Carapax Trionycis place the copper pot, add water logging and do not have face, heat with slow fire; Keep little and boiled 8-24 hour, inclining decocting liquid, successively fries in shallow oil 3-5 time altogether, is degree until abundant fried glue juice, filters clarification then; Merge glue that each time fried in shallow oil, remove by filter precipitated impurities, concentrate receipts glue, the glue juice after filtering is put in the copper pot concentrated constantly stirring with the slow fire heating; In case coking is waited to be concentrated into " extension flag " back and is received glue, in glue impouring gel dish, make it the nature condensation, solidify for accelerating glue juice; Also can add people 5%-10% Colla Corii Asini, take out and solidify good blob of viscose, cut into pieces with cutting the glue cutter, subsequent use.10 kilograms of Radix Ginsengs, 10 kilograms of 10 Radixs Astragali, 28 kilograms of Herba Hedyotidis Diffusaes, 15 kilograms of the Radix Paeoniae Albas, 9 kilograms of Radix Aconiti Lateralis Preparatas; 8 kilograms of 10 kilograms of the Cortexs Eucommiae and Radix Glycyrrhizae Preparatas use the ethanol extraction twice of volumetric concentration as 50-90%, merge extractive liquid; Reclaim ethanol then, concentrate, add Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, and concentrate dry extract, mix homogeneously; Add the pure water of 5 times of proper amount of edible antiseptic and the total drug weight of adding dry extract, process oral liquid, obtain product.

The anti-hepatocarcinoma transplanted tumor test of Chinese medicine preparation of the present invention

Below be the application example of Chinese medicine preparation of the present invention:

One, inquires into the inhibitory action of Chinese medicine preparation of the present invention to hepatocarcinoma transplanted tumor.

The inventor tests discovery, and the preparation of processing with Radix Ginseng, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Carapax et Plastrum Testudinis, Herba Hedyotidis Diffusae, Carapax Trionycis, the Radix Paeoniae Alba, Radix Aconiti Lateralis Preparata, the Cortex Eucommiae, Radix Glycyrrhizae Preparata can have stronger inhibitory action to mice live body ascites H22 HCC

1 materials and methods

1.1 Kunming mouse, in 6 ages in week, 21, male BALB/C mice is divided into 3 groups at random, and 7 every group, body weight 18～22g purchases the Experimental Animal Center in Guangxi Medical University.

1.2 cell culture

Ascitic type H22 hepatoma cell strain is provided by Colleges Of Traditional Chinese Medicine Of Guangxi, is incubated in RPMI1640 (GIBCO company) culture fluid that contains 10% hyclone, places CO ₂Concentration is 5%, relative humidity is 95%, temperature is to cultivate in 37 ℃ the cell culture incubator.Cultivate 48 behind the passage, the attached cell of 96h is the exponential phase cell, gets the back 48h cell that goes down to posterity.

1.3 medicine, the medicine of (A group) the foregoing description 5.With Radix Astragali Radix Ginseng aqueous extract, each 100 gram of Radix Astragali Radix Ginseng add water 1000 grams and boiled 2 hours (B group); (C group) 1% ethanol,

1.4 test method, every mice body weight before the record inoculation.Inoculation exponential phase H22 cell is subcutaneous in the right side of mice groin, about 6.0 * 106/ of inoculum concentration.

From inoculation the 6th day, (C group) matched group 5% G/NS solution 0.2～0.5mL intratumor injection D6,8,10,12.The medicine of embodiment 5 (A group) 1g/mL 0.2～0.5mL intratumor injection D6,8,10,12; , (B group) is with Radix Astragali Radix Ginseng aqueous extract 1g/mL 0.2～0.5mL intratumor injection D6,8,10,12; From inoculation every range estimation amount right side of mice groin subcutaneous transplantation tumor size on the 4th.Tumor body volume V/4 * a * b ²(a is a major diameter, and b is a minor axis).Inoculate the 12nd, take off the neck marrow after mice is weighed and put to death tumor-bearing mice, peel off that to take by weighing tumor behind the tumor body heavy.Tumour inhibiting rate=(matched group tumor weigh an administration group tumor heavy)/matched group tumor heavy * 100%: coefficient of concordance=matched group tumor weight * drug combination group tumor is heavy/(single medicine group 1. tumor weight * single medicine group 2. tumor weight); When tumour inhibiting rate＞40%, P＜0.05 is judged has antitumor action; When coefficient of concordance＜1 for concertedness is arranged.

1.5 statistical method

Expression that all data are all used

; Adopt Microsoft excel software that data are done statistical procedures, adopt variance analysis.

2 results (seeing table 1)

Table 1 Chinese medicine preparation of the present invention is to the influence of H22 transplanted tumor

Medicine of the present invention and Radix Astragali ginseng aqueous extract are to the influence

of H22 transplanted tumor

Annotate: P＜0.05

Two, discuss

The inventor tests discovery,

1, the preparation of processing with Radix Ginseng of the present invention, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Carapax et Plastrum Testudinis, Herba Hedyotidis Diffusae, Carapax Trionycis, the Radix Paeoniae Alba, Radix Aconiti Lateralis Preparata, the Cortex Eucommiae, Radix Glycyrrhizae Preparata also can make mice live body ascites H22 HCC necrosis or apoptosis phenomenons such as gross distortion, after birth are imperfect, foaming, karyopyknosis occur.

2, test is also pointed out and is had the effect of blood circulation promoting and blood stasis dispelling, hard masses softening and resolving, heat-clearing and toxic substances removing, the body resistance strengthening and constitution consolidating with Radix Ginseng of the present invention, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Carapax et Plastrum Testudinis, Herba Hedyotidis Diffusae, Carapax Trionycis, the Radix Paeoniae Alba, Radix Aconiti Lateralis Preparata, the Cortex Eucommiae, Radix Glycyrrhizae Preparata extract; Antitumor action possibly be that the function through the enhancing body cell immune system realizes; Not only help to kill tumor cell, also help the side effect in the ameliorate tumor chemotherapy.

The treatment of cancer instance.

Deep * *, the man, nineteen forty-two gives birth to, and Nanning people from city finds abdominal pain; Belly distending pain all day, the soreness of waist, the inspection liver has lump, and biopsy is a hepatocarcinoma, does hepatectomy in certain tumour hospital at the beginning of 2009; Cut 1/4 liver, after 5 months, find abdominal pain once more, hospital required patient to do chemotherapy at that time, and patient disagrees with; After change the granule of taking medicine embodiment three of the present invention, every day twice, each 10 the gram, take belly distending pain after 20 days; Soreness of waist symptom takes a turn for the better, take one month after, belly distending pain improving situation was survived 1 year.

Lee * *, the woman, nineteen fifty-nine gives birth to, the teacher of Liuzhou technical school, in December, 2009 is suffered from hepatocarcinoma; Workers' hospital's hospitalization in Liuzhou, inspection is shifted, and the abdominal part distending pain is not operated on and is performed an operation, and adopts conservative treatment; Family members require to take the Chinese medicinal tablet of the embodiment of the invention two, and every day 3-4 time, totally 20 grams are taken the aftersensation in January and got that sb.'s illness took a favorable turn, the improvement of abdominal part distending pain symptom; Can arrive outdoor activity, continue to take 2 months, but take a walk to the outside every day, work can do housework.

Flood * *, life in 1967, people from Wuzhou, Guangxi, on June 10th, 2009 was suffered from pulmonary carcinoma at examination in hospital; Right lung pain, dyspnea is not in hospital owing to lack of capital, and takes the capsule of the embodiment of the invention one; Every day 3-4 time, totally 15 grams are taken the aftersensation in January and are got that sb.'s illness took a favorable turn; Breathe smooth and easy, present right lung pain, dyspneic symptom takes a turn for the better.

The present invention is except having the better therapeutic effect hepatocarcinoma, a tree name inventor preliminary test, and this Chinese medicine preparation can play the effect that prevents and treats intestinal cancer.Clinical trial is still among test.

Claims (4)

1. Chinese medicine preparation that suppresses hepatocarcinoma, it is characterized in that: its effective ingredient is processed by following parts by weight of traditional Chinese medicines raw material:

Radix Ginseng 8-15 Radix Astragali 3-10 Rhizoma Atractylodis Macrocephalae 3-10 Carapax et Plastrum Testudinis 10-20 Herba Hedyotidis Diffusae 15-30

Carapax Trionycis 3-10 Radix Paeoniae Alba 8-15 Radix Aconiti Lateralis Preparata 3-10 Cortex Eucommiae 3-10 Radix Glycyrrhizae Preparata 3-10.

2. Chinese medicine preparation that suppresses hepatocarcinoma, it is characterized in that: its effective ingredient is further processed by following parts by weight of traditional Chinese medicines raw material:

Radix Ginseng 15 Radixs Astragali 10 Rhizoma Atractylodis Macrocephalaes 10 Carapax et Plastrum Testudiniss 20 Herba Hedyotidis Diffusaes 30

Carapax Trionycis 10 Radix Paeoniae Albas 15 Radix Aconiti Lateralis Preparatas 10 Cortexs Eucommiae 10 Radix Glycyrrhizae Preparatas 10.

3. the method for preparing of the Chinese medicine preparation of inhibition hepatocarcinoma as claimed in claim 1 is characterized in that: gets Radix Ginseng and is crushed into powder, and subsequent use; Carapax et Plastrum Testudinis, Carapax Trionycis are placed the copper pot, add water logging and do not have face, with the slow fire heating, keep little and boiled 8-24 hour, inclining decocting liquid; Successively fry in shallow oil 3-5 time altogether, be degree, filter clarification then, merge glue that each time fried in shallow oil, remove by filter precipitated impurities until abundant fried glue juice; Concentrate to receive glue, the glue juice after filtering is put in the copper pot concentrated, constantly stir, in case coking with the slow fire heating; Wait to be concentrated into " extension flag " back and receive glue, in glue impouring gel dish, make it the nature condensation, solidify for accelerating glue juice; Also can add the 5%-10% Colla Corii Asini, take out and solidify good blob of viscose, cut into pieces with cutting the glue cutter, subsequent use; The Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Herba Hedyotidis Diffusae, the Radix Paeoniae Alba, Radix Aconiti Lateralis Preparata, the Cortex Eucommiae and Radix Glycyrrhizae Preparata use the ethanol extraction twice of volumetric concentration as 50-90%, and merge extractive liquid, is subsequent use; Concentrate after filtering, reclaim ethanol then, add Carapax et Plastrum Testudinis, Colla carapax Trionycis juice, also concentrate, get dry extract, merge with above-mentioned Radix Ginseng powder, mix homogeneously adds filler, processes capsule or granule, tablet, obtains product; Perhaps add sucrose or Mel, mix homogeneously is made pill.

4. the application of the Chinese medicine preparation of the described inhibition hepatocarcinoma of claim 1 aspect the medicine of preparation treatment hepatocarcinoma.