CN109157572A - The application of Rosa Damascana prevention and treatment anxiety disorder - Google Patents
The application of Rosa Damascana prevention and treatment anxiety disorder Download PDFInfo
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- CN109157572A CN109157572A CN201811045759.6A CN201811045759A CN109157572A CN 109157572 A CN109157572 A CN 109157572A CN 201811045759 A CN201811045759 A CN 201811045759A CN 109157572 A CN109157572 A CN 109157572A
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- rosa damascana
- essential oil
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- 235000011449 Rosa Nutrition 0.000 title claims abstract description 71
- 208000019901 Anxiety disease Diseases 0.000 title claims abstract description 46
- 230000002265 prevention Effects 0.000 title claims abstract description 7
- GLZPCOQZEFWAFX-UHFFFAOYSA-N Geraniol Chemical compound CC(C)=CCCC(C)=CCO GLZPCOQZEFWAFX-UHFFFAOYSA-N 0.000 claims abstract description 24
- 230000036506 anxiety Effects 0.000 claims abstract description 23
- 230000000694 effects Effects 0.000 claims abstract description 20
- 239000000203 mixture Substances 0.000 claims abstract description 13
- QMVPMAAFGQKVCJ-UHFFFAOYSA-N citronellol Chemical compound OCCC(C)CCC=C(C)C QMVPMAAFGQKVCJ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 241000220317 Rosa Species 0.000 claims abstract description 11
- QMVPMAAFGQKVCJ-SNVBAGLBSA-N (R)-(+)-citronellol Natural products OCC[C@H](C)CCC=C(C)C QMVPMAAFGQKVCJ-SNVBAGLBSA-N 0.000 claims abstract description 6
- GLZPCOQZEFWAFX-YFHOEESVSA-N Geraniol Natural products CC(C)=CCC\C(C)=C/CO GLZPCOQZEFWAFX-YFHOEESVSA-N 0.000 claims abstract description 6
- 239000005792 Geraniol Substances 0.000 claims abstract description 6
- GLZPCOQZEFWAFX-JXMROGBWSA-N Nerol Natural products CC(C)=CCC\C(C)=C\CO GLZPCOQZEFWAFX-JXMROGBWSA-N 0.000 claims abstract description 6
- JGQFVRIQXUFPAH-UHFFFAOYSA-N beta-citronellol Natural products OCCC(C)CCCC(C)=C JGQFVRIQXUFPAH-UHFFFAOYSA-N 0.000 claims abstract description 6
- 235000000484 citronellol Nutrition 0.000 claims abstract description 6
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- 150000001875 compounds Chemical class 0.000 claims description 2
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- 238000012360 testing method Methods 0.000 abstract description 29
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- 239000000463 material Substances 0.000 description 7
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- 238000012545 processing Methods 0.000 description 6
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- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 4
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 230000000049 anti-anxiety effect Effects 0.000 description 2
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- 229910052742 iron Inorganic materials 0.000 description 2
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 description 2
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- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 description 1
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 description 1
- SVUOLADPCWQTTE-UHFFFAOYSA-N 1h-1,2-benzodiazepine Chemical compound N1N=CC=CC2=CC=CC=C12 SVUOLADPCWQTTE-UHFFFAOYSA-N 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 206010002869 Anxiety symptoms Diseases 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 240000007436 Cananga odorata Species 0.000 description 1
- 206010013654 Drug abuse Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 244000178870 Lavandula angustifolia Species 0.000 description 1
- 235000010663 Lavandula angustifolia Nutrition 0.000 description 1
- 235000010654 Melissa officinalis Nutrition 0.000 description 1
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- 102000004108 Neurotransmitter Receptors Human genes 0.000 description 1
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- 240000006066 Rosa rugosa Species 0.000 description 1
- 235000000659 Rosa rugosa Nutrition 0.000 description 1
- 241001529742 Rosmarinus Species 0.000 description 1
- 244000143590 Salvia chinensis Species 0.000 description 1
- 235000007154 Salvia chinensis Nutrition 0.000 description 1
- 235000005794 Salvia japonica Nutrition 0.000 description 1
- 206010039897 Sedation Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- VIROVYVQCGLCII-UHFFFAOYSA-N amobarbital Chemical class CC(C)CCC1(CC)C(=O)NC(=O)NC1=O VIROVYVQCGLCII-UHFFFAOYSA-N 0.000 description 1
- 239000002249 anxiolytic agent Substances 0.000 description 1
- 230000000949 anxiolytic effect Effects 0.000 description 1
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- 239000002003 electrode paste Substances 0.000 description 1
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- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000001102 lavandula vera Substances 0.000 description 1
- 235000018219 lavender Nutrition 0.000 description 1
- 229930007744 linalool Natural products 0.000 description 1
- 239000000865 liniment Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/22—Anxiolytics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
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- Public Health (AREA)
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- Polymers & Plastics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides a kind of applications of Rosa Damascana prevention and treatment anxiety disorder, and provide application of the Rosa Damascana in the composition that preparation has effects that alleviation anxiety.Rosa Damascana of the present invention is to use extraction by steam distillation from rose, include each component of following mass content in the Rosa Damascana: citronellol relative amount is 30%-65%, geraniol relative amount is 5%-20%, and nerol relative amount is between 2%-10%.Zoopery and human volunteer's test show that Rosa Damascana of the invention has angst resistance effect, can alleviate the anxiety-like behavior of mouse and the anxiety of volunteer.
Description
Technical field
The present invention relates to essential oil technical fields, and in particular to a kind of application of Rosa Damascana prevention and treatment anxiety disorder.
Background technique
Anxiety is universal to be present in normal person, is a kind of nervous unhappy mood of heart, in some trouble
Have in the crowd of disease, crowd's higher level with anxiety.And anxiety disorder is the general name of a major class phrenoblabia, is
One of the main reason for leading to depression and drug abuse.People are mainly using the medicine with sedative-hypnotic effect before 20th century
Object treats anxiety disorder, such as bromide, chloraldurate etc., and this kind of drug has the shortcomings that sedation is strong, side effect is obvious.
The drugs such as the amobarbital class drug, benzodiazepine, the serotonin reuptake inhibitors class that then develop are in the later period
The problems such as strong, dependence that still there are adverse reactions in clinical practice is by force, action is slow.
Up to the present, people are not apparent the understanding of anxiety disorder mechanism still.Hereditary variation and ring
Border factor is considered as causing two big factors of anxiety disorder.It removes outside neurotransmitter receptor target spot, also constantly there is one in recent years
A little new albumen are proved to have close ties with anxiety disorder, this provides new possible approaches for treatment anxiety disorder.
But generally speaking last decade be to the investment of the new drug development of anxiety treating dysfunction it is huge, it is as a result but and unsatisfactory.
The problem of new drug development is made slow progress is removed, people how to be allowed to be ready that the treatment for receiving to be directed to anxiety disorder is also one
Hang-up.In the investigation of a 2001-2005, China is diagnosed in the crowd of certain mental disease, only 8%
Crowd once sought help to professional, and only 5% crowd once went to a doctor in psychiatric department.For being reluctant to receive drug therapy,
Or for the patient groups in the area that treatment mechanism lacks, alleviating anxiety symptom using complementary medicine is feasible way
Diameter.
Use aromatotherapy to treat anxiety disorder as complementary therapy, alleviates the very long history of anxiety.It is many
Essential oil is in the long-term practice that virtue is treated by as " good medicine " for calming down mood, pressure of releiving.Last century the mid-80 is started from,
There are many scientific researches to be demonstrated on human body from lavender, ylang-ylang, rosemary, Salvia japonica, balm and permitted
Mostly citrous natural essential oil has the function of alleviating anxiety.It mainly includes that the Chinese is close that these, which study used evaluation index,
You are manifest anxiety scale (Hamilton Anxiety Scale, HAMA), SF-36 scale, Zung Analysisof Self-rating Anxiety Scale (Self-
Rating Anxiety Scale, SAS) etc. Anxiety Evaluations scale and saliva CGA, heart rate variability, blood pressure, brain electricity
Etc. physical signs.
Using the effect of animal model evaluation plants essential oil and proving at the beginning of the mechanism of action mainly concentrates and start from 21 century.It is small
Mouse (Mus musculus) is to use frequent animal in anxiolytic drugs preclinical study at present.Researcher is small at present
Antianxiety effect of many natural plant essential oils is demonstrated using a variety of models on mouse.These models include based on approach avoidance conflict
Anxiety Model and Anxiety Model based on conflict.The former includes Elevated plus-maze test, spacious field test, the test of light and shade case, hole
Plate test etc., principle is can be for example bright for inherently active dodge in the heuristic process to novel environment based on mouse
The potentially dangerous region of bright, spacious and certain altitude from the ground possibility；The latter it is relatively conventional be Geller-
Seifter conflict test and Vogel drinking-water conflict test.
Rosa Damascana is spent using extraction by steam distillation from rose (Rosa rugosa).Hundred are had more than China more
The plantation history in year, is widely used in food, skin care item, field of health care products at present.The reasons such as kind, the place of production, extracting method
Will cause Chemical Components of Rose Essential obtained, there are larger differences.These chemical components mainly include citronellol, geraniol,
Nerol, linalool and a series of floral wax ingredients.And the difference of chemical component can bring the significant difference of essential oil effect, at present
Not yet there are antianxiety effect and its main composition of document report Rosa Damascana.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of answering for Rosa Damascana prevention and treatment anxiety disorder
With.
The purpose of the present invention is what is be achieved through the following technical solutions:
Have effects that alleviate the application in the composition of anxiety in preparation the present invention provides a kind of Rosa Damascana.
The present invention also provides a kind of application of Rosa Damascana in the composition of preparation prevention and treatment anxiety disorder.
Preferably, the composition includes any one of essential oil, health care product, pharmaceutical composition.
Preferably, the Rosa Damascana uses extraction by steam distillation from rose rose.
Preferably, include each component of following mass content in the Rosa Damascana: citronellol relative amount is 30%-
65%, geraniol relative amount is 5%-20%, and nerol relative amount is 2%-10%.
Preferably, in the composition, the concentration of Rosa Damascana is 10-250 μ l/ml.
Preferably, the essential oil is folk prescription essential oil or compound essential oil；The application form of the essential oil be spraying, capsule,
Any one of granule, aqua, creams, gel.
Preferably, the application form of the health care product is capsule, tablet, granule, powder, oral solution, pill, cream
Any one of frost, gel, spraying.
Preferably, the application form of described pharmaceutical composition be capsule, tablet, granule, powder, oral solution, pill,
Any one of creams, gel, spraying.
Compared with prior art, the present invention have it is following the utility model has the advantages that
The present invention is tested using zoopery and human volunteer shows that a kind of Rosa Damascana is taken the photograph by smelling suction or massage
It is fashionable that there is apparent angst resistance effect, the anxiety of rat, the anxiety-like behavior of mouse and volunteer can be alleviated, this
The Chemical Characteristics of kind Rosa Damascana are: citronellol relative amount 30%-65%, geraniol relative amount 5%-20%,
Nerol relative amount 2%-10%.A kind of new effective ways are provided to anxiety disorder is prevented and treated.
Detailed description of the invention
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention,
Objects and advantages will become more apparent upon:
Fig. 1: mouse is given after chmice acute smells suction (30min) 3 concentration Rosa Damascana A and Rosa Damascana B overhead ten
In the percentage of time of open arms exploration, (open arms are explored percentage of time=open arms explore the time/and (are opened in the test of word labyrinth
Put arm and explore time+closure arm exploration time))；Wherein: * indicates that significant difference p < 0.05 is presented compared with the control group；
Fig. 2: mouse is given after chmice acute smells suction (30min) 3 concentration Rosa Damascana A and Rosa Damascana B overhead ten
In the percentage of time of open arms exploration, (open arms are explored percentage of time=open arms explore number/and (are opened in the test of word labyrinth
Put arm and explore number+closure arm exploration number))；Wherein: * indicates that significant difference p < 0.05 is presented compared with the control group；
Fig. 3: it gives mouse after mouse smells suction (30min/d continues 14d) Rosa Damascana A for a long time and is surveyed in Elevated plus-maze
Percentage of time (the open arms exploration percentage of time=open arms exploration time/(open arms exploration explored in examination in open arms
Time+closure arm explores the time))；Wherein: * indicates that significant difference p < 0.05 is presented compared with the control group；
Fig. 4: it gives mouse after mouse smells suction (30min/d continues 14d) Rosa Damascana A for a long time and is surveyed in Elevated plus-maze
Percentage of time (the open arms exploration percentage of time=open arms exploration number/(open arms exploration explored in examination in open arms
Number+closure arm explores number))；Wherein: * indicates that significant difference p < 0.05 is presented compared with the control group；
Fig. 5: give mouse smell after suction (30min/d, continue 14d) Rosa Damascana A for a long time mouse in the test of light and shade case
Percentage of time that camera-lucida is explored is (when camera-lucida explores percentage of time=camera-lucida and explores total exploration of time/in light and shade case
Between)；Wherein: * indicates that significant difference p < 0.05 is presented compared with the control group；
Fig. 6: give rat smell inhale Rosa Damascana C and Rosa Damascana D after, rat Elevated plus-maze test in opening
The percentage of time that arm is explored (explore percentage of time=open arms and explore time/(open arms exploration time+closure by open arms
Arm explores the time))；Wherein: * indicates that significant difference p < 0.05 is presented compared with the control group；
Fig. 7: give chmice acute smell inhale Rosa Damascana E, F, G, H after, mouse Elevated plus-maze test in opening
The percentage of time that arm is explored (explore percentage of time=open arms and explore time/(open arms exploration time+closure by open arms
Arm explores the time))；Wherein: * indicates that significant difference p < 0.05 is presented compared with the control group；
Fig. 8: MAT stress after, give volunteer's Rosa Damascana A (3%, v/v) and 100% Sweet Almond Oil massage front and back aspiration
The variation of person's heart rate low frequency power LF, high frequency power HF and low frequency high frequency ratio LF/HF；Wherein: at * expression and Sweet Almond Oil
Reason group is compared to presentation significant difference p < 0.05.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, several changes and improvements can also be made.These belong to the present invention
Protection scope.
Embodiment 1
It present embodiments provides using the acute and long-term two kinds of Rosa Damascanas of suction of smelling of Elevated plus-maze test evaluation to small
The influence of mouse anxiety behavior.
1.1 materials and methods
Rosa Damascana A is to extract to obtain from fresh rose flower by steam distillation, and Rosa Damascana B is to pass through vapor
The way of distillation is extracted from fresh rose flower and is obtained.Diazepam injection (10mg/2mL) is given birth to by Shanghai Xudong Hipu Medicine Co., Ltd
It produces.
The GC-MS (S6890/5973N, USA) that Essential Oil is produced using Agilent company.Specific test
Condition is as follows: chromatographic condition: Supelco18275-06A PTE-5Capillary column (0.25 μm of the μ m of 30.0m × 250)；Journey
Sequence heating: 70 DEG C → (2 DEG C/min) → 180 DEG C → (15 DEG C/min) → 290 DEG C (keeping 15min)；Column flow: 0.8ml；Sample introduction
Amount: 0.2 μ l；Carrier gas: nitrogen；Split ratio: 50:1.Mass Spectrometry Conditions: the Agilent Db-5MS chromatographic column (μ m of 50m × 250 0.25
μm)；Column flow: 1ml/min；Mass range: 29-420AMU；Ionization voltage: 70eV；Electron multiplier voltage (EMV): 1435V；
Ion source temperature: 230 DEG C；Level four bars temperature: 150 DEG C.
Experimental animal is purchased from this Leco Corp. of Shanghai using healthy male ICR mouse, 18-22g.In order to allow experimental animal
It shakes down, is tested again after carrying out 7d adaptive feeding after animal purchase.Animal freely ingests drinking-water during raising, just
Normal 12h day-night cycle, relative humidity 40%-50%, 24 ± 2 DEG C of room temperature.
Animal champignon room is self-control, and having a size of 50 × 50 × 25cm, material is stainless steel and iron, matches transparent glass cover, internal
It is divided into 4 individual compartments, for each compartment having a size of 20 × 20 × 25cm, compartment walls there are small ventilating holes, and Fragrance Lamp is placed in champignon
Cross aisle among room.Using Fragrance Lamp heating diffusion essential oil during champignon, make mouse smelling fragrance.
The angst resistance effect of Rosa Damascana is tested using mouse elevated plus-maze (Elevated plus-maze, EPM).
EPM is the model for investigating animal anxiety with probably high psychological contradiction to the exploration desire of novel environment using animal, small
The time and number that mouse is explored in open arms are more, show that mouse anxiety mood is lower.Elevated plus-maze component is by two phases
It is formed by connecting to open arms (30 × 6cm) and two relatively closed arms (30 × 6 × 15cm) and central area (6 × 6cm), it is entire to be confused
Palace is placed on the bracket away from ground 55cm.It allows mouse freely to explore EPM 5min in experiment, records mouse in open arms and closure
The time and number that arm is explored.
1.2 experiment flow
Mouse is grouped at random, every group 8.Essential oil group: after intraperitoneal injection 0.3ml physiological saline, mouse is placed on animal
Champignon room smells suction corresponding concentration essential oil and (takes 20 μ l of essential oil, 100 μ l, 500 μ l to be dissolved in 2ml Tween 80-aqueous solution respectively, be made
The essential oil solution of 10 μ l/ml, 50 μ l/ml, 250 μ l/ml).Control treatment group is used to be replaced with the Tween 80 of essential oil same volume
Essential oil, remaining processing are identical.Positive control drug uses diazepam (1mg/kg), and mouse is then placed on animal champignon room, smells suction
Blank solution 30min.Experiment is divided into acute smell and inhales and smell for a long time suction experiment.It is acute to smell mouse in suction experiment and carry out once smelling suction
After (time 30min), Behaviors survey is carried out immediately；Mouse daily set time (14:00) progress in suction experiment is smelt for a long time
Incense chamber smells suction (time 30min), 14d is smelt suck complete 2h after carry out Elevated plus-maze test.
1.3 experimental result
After chmice acute smells the Rosa Damascana for inhaling various concentration, compared with control treatment, low concentration Rosa Damascana A can be shown
It writes and improves percentage of time and percentage of time that mouse is explored in open arms, and improve degree and positive control drug diazepam phase
As (see attached drawing 1 and Fig. 2).Smell inhale Rosa Damascana B will not the anxiety behavior on mouse make a significant impact.Show acute to smell suction rose
Rare essential oil A can reduce mouse anxiety behavior.
It is persistently smelt to mouse and inhales low concentration Rosa Damascana A after 14 days, compared with control treatment group, low concentration Rosa Damascana A
Percentage of time and percentage of time (p < 0.05) that mouse is explored in open arms can be significantly improved (see attached drawing 4 and Fig. 5).Table
The bright suction Rosa Damascana A that smells for a long time can reduce mouse anxiety behavior.
Several main chemical compositions of Rosa Damascana A and B are as follows:
Embodiment 2
The present embodiment smells the effect for inhaling Rosa Damascana A reduction mouse anxiety behavior using the test evaluation of light and shade case for a long time.
2.1 materials and methods
Rosa Damascana A is extracted from rose by steam distillation and is obtained.Diazepam injection (10mg/2mL) by
Shanghai Xudong Hipu Medicine Co., Ltd's production.
Experimental animal uses ICR hero mouse, and 18-22g is purchased from this Leco Corp. of Shanghai.In order to allow experimental animal to adapt to new ring
Border is tested again after carrying out 7d adaptive feeding after animal purchase.Animal freely ingests drinking-water during raising, normal 12h daytime
Night circulation, relative humidity 40%-50%, 24 ± 2 DEG C of room temperature.
Animal champignon room is self-control, and having a size of 50 × 50 × 25cm, material is stainless steel and iron, matches transparent glass cover, internal
It is divided into 4 individual compartments, for each compartment having a size of 20 × 20 × 25cm, compartment walls there are small ventilating holes, and Fragrance Lamp is placed in champignon
Cross aisle among room.Using Fragrance Lamp heating diffusion essential oil during champignon, make mouse smelling fragrance.
The angst resistance effect of Rosa Damascana is tested using light and shade case (Light-Dark Box, LDB), and light and shade case is having a size of 55
× 27 × 30cm, wherein camera-lucida is having a size of 27 × 27 × 30cm, and camera bellows is having a size of 18 × 27 × 30cm；Camera-lucida bottom middle is shone
Degree is 500LuX.In experimentation, mouse is put into camera-lucida, the plate among light and shade case is extracted out, is allowed to freely probe into 5min,
Record the behavioral indexes of mouse in 5min.It records mouse and explores percentage of time (Light box time, LT%) in camera-lucida
And mouse explores the percentage (Light box distance, LD%) of distance in camera-lucida.
Chemical Composition of The Essential Oil detection method is the same as embodiment 1.
2.2 experiment flow
Mouse is grouped at random, every group 8.Essential oil group: after intraperitoneal injection 0.3ml physiological saline, mouse is placed on animal
Champignon room smells suction Rosa Damascana A and (takes 20 μ l Rosa Damascana A to be dissolved in 2ml Tween 80-aqueous solution, the essential oil of 10 μ l/ml is made
Solution).Control group processing replaces essential oil using with the Tween 80 of essential oil same volume, remaining processing is identical.Positive control drug is adopted
With diazepam (1mg/kg) (diazepam group), mouse is then placed on animal champignon room, smells and inhales blank solution 30min.Mouse is every
Its set time (14:00) carry out an incense chamber smell suctions (time 30min), 14d is smelt suck complete 2h after progress light and shade case
Test.
2.3 experimental result
Rosa Damascana A chemical component is as described in example 1 above.
Smelling suction Rosa Damascana A for a long time can be significantly reduced the anxiety behavior of mouse.LDB test in, Rosa Damascana group and
Diazepam group can significantly improve the percentage (p < 0.05) (see Fig. 3) that mouse explores the time in camera-lucida.
Embodiment 3
It present embodiments provides and Rosa Damascana C and Rosa Damascana D drop is inhaled using acute smell of Elevated plus-maze test evaluation
The effect of low rat anxiety behavior.
1.1 materials and methods
Rosa Damascana C and Rosa Damascana D is market purchase, and chemical component is as follows:
Experimental animal uses Healthy female and male SD rat, 200-220g.7d adaptive feeding is carried out after animal purchase
It is tested again afterwards.Animal freely ingests drinking-water during raising, normal 12h day-night cycle, relative humidity 40%-50%, room temperature
24±2℃。
Animal champignon room is self-control, and champignon room: having a size of 100cm × 100cm × 100cm, surrounding plate is transparent.With center
Hollow out partition divides two sides, and fragrance diffuser is placed in side, and experimental animal is placed in side.Expanded during champignon using Fragrance Lamp heating
Essential oil is dissipated, animal smelling fragrance is made.
The angst resistance effect of Rosa Damascana is tested using Elevated plus-maze.Rat is allowed freely to explore EPM in experiment
5min, time and number of the record rat in open arms and closure arm exploration.
1.2 experiment flow
Rat is grouped at random, every group 8, male and female each 4.Rat is placed on animal champignon room by essential oil group, is smelt suction and is corresponded to
Concentration essential oil (takes 100 μ l of essential oil, 600 μ l to be dissolved in 2ml Tween 80-aqueous solution respectively, the essence of 50 μ l/ml, 300 μ l/ml is made
Oil solution).Control treatment group, which is used, replaces essential oil with the Tween 80 of essential oil same volume, remaining processing is identical.Then by rat
It is placed on animal champignon room, it is acute to smell after rat in suction experiment smells and inhale essential oil 15min, Elevated plus-maze test is carried out immediately.
1.3 experimental result
The Rosa Damascana C for smelling suction low concentration can significantly improve the percentage of time that mouse is explored in open arms, smell suction rose
Rare essential oil D will not the anxiety behavior on rat make a significant impact (see attached drawing 6).The experimental results showed that smelling the rose for inhaling low concentration
Essential oil C has angst resistance effect.
Embodiment 4
1.1 materials and methods
Rosa Damascana E, F, G, H are market purchase, and chemical component is as follows:
Experimental animal, champignon room and Elevated plus-maze test are the same as embodiment 1.
1.2 experiment flow
Mouse is grouped at random, every group 8.Essential oil group: being placed on animal champignon room for mouse, smells suction Rosa Damascana and (takes 20 μ
L Rosa Damascana A is dissolved in 2ml Tween 80-aqueous solution, and the essential oil solution of 10 μ l/ml is made).Control group processing uses and essential oil
The Tween 80 of same volume replaces essential oil, remaining processing is identical.Mouse carries out overhead ten after completing to smell suction essential oil 30min at once
The test of word labyrinth.
1.3 experimental result
The percentage of time that mouse is explored in open arms can be significantly improved by smelling suction Rosa Damascana F, G, smell suction Rosa Damascana
E, H will not the anxiety behavior on rat make a significant impact (see attached drawing 7).Have the experimental results showed that smelling and inhaling Rosa Damascana F, G
Angst resistance effect.
Embodiment 5
Present embodiments provide the treatment anxiety disorder for using a kind of Rosa Damascana by the way of massage on human body
Effect.
3.1 materials and methods
Volunteer recruiting inclusion criteria: (1) Analysisof Self-rating Anxiety Scale (Self-rating anxiety scale, SAS) score
Higher than the volunteer of 50 points of persons；(2) volunteer's age is in 25-45 one full year of life；(3) volunteer of experiment is participated in without serious systemic disease
Disease, and sign informed consent form.
Quick mental arithmetic stress: it is required that subject is fast and is accurately finished the mental arithmetic topic of 50 four figures plus-minus, subject
Quickly think deeply after hearing topic and quotes answer.If there is mistake in subject, it will informed at once, and be required to re-start
It calculates, until correct.
Heart rate variability (heart rate variability, HRV) measurement: it will test electrode paste and carried on the back in bilateral wrist
Portion keeps quite until test is completed.Measured keeps sitting position in test process.The HRV index of measurement includes that heart rate is low
Frequency power (Low frequency power, LF), high frequency power (High frequency power, HF) and low frequency high frequency
Ratio (LF/HF).The activity of LF (0.04~0.15Hz) expression stomodaeal nervous system；HF (0.15~0.4Hz) indicates parasympathetic
The activity of nervous system；LF/HF indicates sympathetic and parasympathetic activity balance.It is pretherapy and post-treatment to receive essential oil with subject
The difference of HRV value is as evaluation index.
Essential oil massage: allow subject's prostrate on massage bed, face is downward, with two arms hanging on both sides.Essential oil is uniformly smeared
In the back of subject, neck and shoulder.Every subject's applying amount is 5 milliliters, and control treatment group uses 100% dessert almond
Oil, experimental group then use the Rosa Damascana A in above-described embodiment of 3% (v/v), and dilution medium is Sweet Almond Oil.
3.2 experiment flow
Volunteer (n=40) is grouped at random before test, and avoids alcohol, Ni Gu on the day of requiring volunteer to test
The intake of fourth, coffee, drug and any essential oil；Test the previous day sleep at least 7 hours.
When test, subject sits quietly 5min first, measures items HRV index.Next subject MAT stimulation, MAT are given
Items HRV index is further measured after stimulation.Then giving subject's massage essential oil and control group base oil, massage terminates
Subject sits quietly 20min afterwards, measures items HRV index again.With MAT stress after HRV value and receive essential oil/base oil and massage
The difference of HRV value afterwards is as evaluation index.
3.3 experimental result
From figure 8, it is seen that subject receive 3% (v/v) Rosa Damascana A and Sweet Almond Oil massage after, LF and LF/
Downward trend is presented in HF value, and ascendant trend is presented in HF.Wherein compared with Sweet Almond Oil massage group, the LF/ of Rosa Damascana A massage group
HF rate of descent dramatically increases (p < 0.05).
The reflection of this index of LF/HF is sympathetic nerve and parasympathetic balance, is evaluation automatic nervous system
The nervous or most important index of degree of releiving.Usually, anxiety crowd is obviously with cardiac vegetative nerve function of peripheral disorder phenomenon,
Their LF, LF/HF is all significantly higher than normal person under quiescent condition.This experimental results showed that Rosa Damascana A can reduce by
The LF/HF value of examination person has the function of reducing subject's anxiety level.
The present embodiment equally demonstrates the effect of Rosa Damascana C, F, G, and result is suitable with Rosa Damascana A.Illustrate in rose
In rare essential oil, significant anti-coke can be played when chemical component citronellol, geraniol and nerol are in certain content range
The effect of worry.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned
Particular implementation, those skilled in the art can make a variety of changes or modify within the scope of the claims, this not shadow
Ring substantive content of the invention.In the absence of conflict, the feature in embodiments herein and embodiment can any phase
Mutually combination.
Claims (9)
1. a kind of Rosa Damascana has effects that alleviate the application in the composition of anxiety in preparation.
2. a kind of application of Rosa Damascana in the composition of preparation prevention and treatment anxiety disorder.
3. application according to claim 1 or 2, which is characterized in that the composition includes essential oil, health care product, drug
Any one of composition.
4. application according to claim 1 or 2, which is characterized in that the Rosa Damascana is mentioned using steam distillation
It is derived from rose rose.
5. application according to claim 1 or 2, which is characterized in that include following mass content in the Rosa Damascana
Each component: citronellol relative amount is 30%-65%, and geraniol relative amount is 5%-20%, and nerol relative amount is
2%-10%.
6. application according to claim 1 or 2, which is characterized in that in the composition, the concentration of Rosa Damascana is 1-
250μl/ml。
7. application according to claim 3, which is characterized in that the essential oil is folk prescription essential oil or compound essential oil；The essence
The application form of oil is any one of spraying, capsule, granule, aqua, creams, gel.
8. application according to claim 3, which is characterized in that the application form of the health care product be capsule, tablet,
Any one of granula, powder, oral solution, pill, creams, gel, spraying.
9. application according to claim 3, which is characterized in that the application form of described pharmaceutical composition is capsule, piece
Any one of agent, granule, powder, oral solution, pill, creams, gel, spraying.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113908210A (en) * | 2021-10-26 | 2022-01-11 | 湖北优巴克生物科技有限公司 | Traditional Chinese medicine anti-anxiety bag and preparation method thereof |
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| CN106581364A (en) * | 2016-12-22 | 2017-04-26 | 无限极（中国）有限公司 | Essential oil composition and applications thereof in preparing preparations capable of alleviating anxiety |
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| CN106581364A (en) * | 2016-12-22 | 2017-04-26 | 无限极（中国）有限公司 | Essential oil composition and applications thereof in preparing preparations capable of alleviating anxiety |
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Application publication date: 20190108 |