CN106578717B - Preparation and feeding method of coral artificial culture slow-release granular bait - Google Patents
Preparation and feeding method of coral artificial culture slow-release granular bait Download PDFInfo
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- 235000014653 Carica parviflora Nutrition 0.000 title claims abstract description 58
- 239000004512 granular bait Substances 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 12
- 241000243321 Cnidaria Species 0.000 title description 40
- 239000000843 powder Substances 0.000 claims abstract description 47
- 241000190984 Rhodospirillum rubrum Species 0.000 claims abstract description 18
- 239000011782 vitamin Substances 0.000 claims abstract description 13
- 229940088594 vitamin Drugs 0.000 claims abstract description 13
- 235000013343 vitamin Nutrition 0.000 claims abstract description 13
- 229930003231 vitamin Natural products 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 11
- 238000009395 breeding Methods 0.000 claims abstract description 10
- 230000001488 breeding effect Effects 0.000 claims abstract description 10
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- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims abstract description 9
- 241000237536 Mytilus edulis Species 0.000 claims abstract description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000000292 calcium oxide Substances 0.000 claims abstract description 9
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 9
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims abstract description 9
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims abstract description 9
- 235000021323 fish oil Nutrition 0.000 claims abstract description 9
- 235000020638 mussel Nutrition 0.000 claims abstract description 9
- 210000002149 gonad Anatomy 0.000 claims abstract description 8
- 241000196251 Ulva arasakii Species 0.000 claims abstract description 7
- 238000005303 weighing Methods 0.000 claims abstract description 5
- 238000001238 wet grinding Methods 0.000 claims abstract description 5
- 241000195493 Cryptophyta Species 0.000 claims abstract description 4
- 244000132059 Carica parviflora Species 0.000 claims abstract 15
- 241001275783 Mysida Species 0.000 claims abstract 2
- 238000001035 drying Methods 0.000 claims description 7
- 239000008187 granular material Substances 0.000 claims description 7
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 7
- 241000159660 Nannochloropsis oculata Species 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 4
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 4
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 4
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000002002 slurry Substances 0.000 claims description 4
- 230000003068 static effect Effects 0.000 claims description 4
- 241000224474 Nannochloropsis Species 0.000 claims description 3
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 2
- 241000233866 Fungi Species 0.000 claims description 2
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 2
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims description 2
- 235000019270 ammonium chloride Nutrition 0.000 claims description 2
- 229960002685 biotin Drugs 0.000 claims description 2
- 235000020958 biotin Nutrition 0.000 claims description 2
- 239000011616 biotin Substances 0.000 claims description 2
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 claims description 2
- 229960002079 calcium pantothenate Drugs 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 229960000304 folic acid Drugs 0.000 claims description 2
- 235000019152 folic acid Nutrition 0.000 claims description 2
- 239000011724 folic acid Substances 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 229960003512 nicotinic acid Drugs 0.000 claims description 2
- 235000001968 nicotinic acid Nutrition 0.000 claims description 2
- 239000011664 nicotinic acid Substances 0.000 claims description 2
- 238000000643 oven drying Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 229960002477 riboflavin Drugs 0.000 claims description 2
- 235000019192 riboflavin Nutrition 0.000 claims description 2
- 239000002151 riboflavin Substances 0.000 claims description 2
- 238000011218 seed culture Methods 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- 239000001632 sodium acetate Substances 0.000 claims description 2
- 235000017281 sodium acetate Nutrition 0.000 claims description 2
- 235000019157 thiamine Nutrition 0.000 claims description 2
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims description 2
- 229960003495 thiamine Drugs 0.000 claims description 2
- 239000011721 thiamine Substances 0.000 claims description 2
- 239000011715 vitamin B12 Substances 0.000 claims description 2
- 239000011726 vitamin B6 Substances 0.000 claims description 2
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000002244 precipitate Substances 0.000 claims 1
- 238000012851 eutrophication Methods 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 4
- 235000013312 flour Nutrition 0.000 abstract description 2
- 241000195649 Chlorella <Chlorellales> Species 0.000 abstract 1
- 235000002723 Dioscorea alata Nutrition 0.000 description 6
- 235000005362 Dioscorea floribunda Nutrition 0.000 description 6
- 235000004868 Dioscorea macrostachya Nutrition 0.000 description 6
- 235000005361 Dioscorea nummularia Nutrition 0.000 description 6
- 235000005360 Dioscorea spiculiflora Nutrition 0.000 description 6
- 235000004879 dioscorea Nutrition 0.000 description 6
- 241001328003 Mysidacea Species 0.000 description 4
- 241000242757 Anthozoa Species 0.000 description 3
- 241000180279 Chlorococcum Species 0.000 description 3
- 235000007056 Dioscorea composita Nutrition 0.000 description 3
- 235000009723 Dioscorea convolvulacea Nutrition 0.000 description 3
- 235000006350 Ipomoea batatas var. batatas Nutrition 0.000 description 3
- 241000196252 Ulva Species 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 206010001557 Albinism Diseases 0.000 description 1
- 241000143060 Americamysis bahia Species 0.000 description 1
- 235000002722 Dioscorea batatas Nutrition 0.000 description 1
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 1
- 240000001811 Dioscorea oppositifolia Species 0.000 description 1
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 1
- 241000190967 Rhodospirillum Species 0.000 description 1
- -1 compound vitamin Chemical class 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to a preparation and feeding method of slow-release bait for artificial coral culture. The method is characterized by comprising the following steps: 1) weighing Mysidacea, mussel gonad, Ulva lactuca and herba Zosterae Marinae, adding appropriate amount of seawater, wet grinding into thick paste, adding calcium oxide, fish oil, Chlorella viridis algae powder, yeast powder, Rhodospirillum rubrum powder, vitamins, and MgS04The raw materials of the carboxymethyl cellulose and the yam flour are added with a proper amount of seawater and mixed evenly to form a wet-mass mixture, and the wet-mass mixture is extruded and granulated by a granulator and dried in the air or hot air to form the slow-release granular bait for artificial coral breeding; 2) and during feeding, the slow-release granular bait is filled in the net bag and is immersed in water to be fixed at the position 150-200 mm above the coral spikelets. The slow-release bait is approximately the same as the predation rate of the coral by adjusting the slow-release rate, so that the utilization rate of the bait is improved, the cost of the bait is reduced, and the water quality eutrophication of the coral culture water tank environment is reduced.
Description
Technical Field
The invention belongs to the technical field of artificial culture of marine organism coral. In particular to a preparation and feeding method of slow-release bait for artificial coral culture.
Background
With the development of the culture of living corals in the commercial aquarium market, the artificial breeding of the corals is one of the main ways to solve the contradiction between the endangered wild coral resources and the continuously growing demand of the corals in the commercial aquarium market. The current data literature reports: few domestic coral breeding researches meeting the requirements of the aquarium market are reported, and the growth influence of physicochemical factors such as illumination, temperature, salinity, pH value and the like on the asexual propagation segmentation amplification mode can be controlled under the condition of an artificial simulated marine ecosystem for coral breeding in the aquarium market. Artificial feed for coral and the feeding method of the feed have great relationship on the success of the coral vegetative propagation in a division and amplification mode. Some studies show that most commercial artificial baits and wrong feeding methods cannot improve the growth rate of the coral, but can cause water eutrophication and coral albinism. Due to the fixed position mode of the coral, the bait feeding process is impossible to swim and prey, so that a large amount of residual bait is generated, the water eutrophication is caused, and the cultivation cost is increased.
Disclosure of Invention
The invention develops the slow-release granular bait for artificial coral culture, which is placed in a coral predation range area, and can slowly dissolve and release the bait according to the coral predation rate, thereby achieving the purposes of improving the bait utilization efficiency, saving the culture cost and reducing the water eutrophication.
The technical scheme adopted for realizing the purpose of the invention is as follows:
1. formula of slow-release granular bait for artificial coral culture
300-500 g of mysid
Mussel gonad 400-500 g
5-8 g of fish oil
200-250 g ulva lactuca
200-250 g of kelp
200-250 g of nannochloropsis algae powder
40-60 g of yeast powder
50-100 g of rhodospirillum rubrum powder
5-7 g of vitamin
MgS04 1～2g
1.5-2.5 g of calcium oxide
25-30 g of carboxymethyl cellulose
25-30 g of yam powder
2. The preparation and feeding method of the coral artificial breeding slow-release bait comprises the following steps:
weighing Mysidacea, mussel gonad, Ulva lactuca and herba Zosterae Marinae according to the above formula proportion, adding appropriate amount of seawater, wet grinding into thick slurry, adding calcium oxide, fish oil, Chlorococcus microalga powder, yeast powder, Rhodospirillum rubrum powder, and vitamin MgS04The coral artificial culture slow-release granular bait is prepared by the steps of uniformly mixing carboxymethyl cellulose and yam powder raw materials with a proper amount of seawater to form a wet-mass mixture, extruding and granulating the wet-mass mixture material by using a granulator, wherein the diameter of the granules is 30-50 mm, and naturally airing or drying the granules by hot air at 50 ℃ to form the coral artificial culture slow-release granular bait.
When feeding, 8: turning off the artificial light source at 00 hours, 8: turning off the wave raising equipment at 30 hours; meanwhile, the slow-release granular bait prepared by the invention is filled in a mesh bag at a ratio of 80 g/bag, and then the mesh bag is immersed in water and fixed at a position 150-200 mm above the small coral spikes. With the gradual dissolution of the slow-release granular baits in the mesh bag, the baits can be observed to be slowly released and preyed by coral tentacles, after the slow-release duration time is 30-40 minutes, the mesh bag is removed, and after 30 minutes, the artificial light source and the wave raising equipment are restarted. At a rate of 0.2 m22 mesh bags are fixed in the area of the coral, and the residual bait with the bottom sinking is removed on the 2 nd day.
Compared with the existing coral artificial bait and feeding technology, the coral artificial breeding slow-release bait has the same predation rate as coral by adjusting the slow-release rate. Improve the utilization rate of the bait, reduce the cost of the bait and reduce the water quality eutrophication of the water tank environment for coral culture. In addition, the preparation method is suitable for industrial production.
The Rhodospirillum rubrum (A), (B) and (C)Rhodospirillum rubrum) The preparation method of the fungus powder comprises the following steps: the 3L of seed culture solution comprises the following components in percentage by weight: 0.2% of ammonium chloride, 0.8% of sodium bicarbonate, 0.1% of dipotassium hydrogen phosphate, 0.40% of sodium acetate, 0.02% of magnesium sulfate and the balance of water; adjusting the pH value of the culture solution to 7.8, inoculating rhodospirillum rubrum into the culture solution, and performing static culture under natural illumination. The static culture conditions were: the culture temperature is 30-32 ℃, and the culture time is 96-120 hours. When the culture is finished, the rhodospirillum rubrum seed liquid contains more than or equal to 20 hundred million rhodospirillum rubrum bacteria per milliliter. Centrifuging to precipitate thallus, and oven drying at 45-55 deg.C to obtain Rhodospirillum rubrum powder.
The strain Rhodospirillum rubrumRhodospirillum rubrumNumbering: 1.3369 purchased from China general microbiological culture Collection center.
The nannochloropsis oculata (A) and (B)Nannnochloropsis gaditana) The preparation method of the algae powder comprises the following steps: chlorococcum pseudonanum (A. pseudonannochloris) ((A. pseudonannochloris))Nannnochloropsis gaditana) After the seeds are cultured, a centrifuge takes the precipitated alga and dries the alga into the nannochloropsis oculata alga powder for standby by hot air at the temperature of 45-55 ℃. Nannochloropsis sp powder can also be purchased from the market.
The compound vitamin comprises the following components in percentage by weight: thiamine 26mg, riboflavin 105 mg, nicotinic acid 510 mg, calcium pantothenate 105 mg, vitamin B6105 mg of vitamin B120.26 mg, biotin 1.2 mg and folic acid 12 mg.
The preparation of the yam powder comprises the following steps: slicing rhizoma Dioscoreae, sun drying, pulverizing, and sieving with 200 mesh sieve to obtain rhizoma Dioscoreae powder. The other raw auxiliary materials can be purchased in the market.
Example 1
3. Formula of slow-release granular bait for artificial coral culture
Shrimp 300g
Mussel sexual gland 500g
Fish oil 8g
Ulva 250g
Kelp (200 g)
Chlorococcum minitans powder 200g
Yeast powder 60g
Rhodospirillum rubrum powder 80g
Vitamin 7g
MgS04 1g
Calcium oxide 2.5g
Carboxymethyl cellulose 25g
30g of yam powder
4. The preparation and feeding method of the coral artificial breeding slow-release bait comprises the following steps:
weighing Mysidacea, mussel gonad, Ulva lactuca and herba Zosterae Marinae according to the above formula proportion, adding appropriate amount of seawater, wet grinding into thick slurry, adding calcium oxide, fish oil, Chlorococcus microalga powder, yeast powder, Rhodospirillum rubrum powder, and vitamin MgS04The coral artificial culture slow-release granular bait is prepared by uniformly mixing carboxymethyl cellulose, yam flour raw materials and a proper amount of seawater into a wet-mass mixture, extruding and granulating the wet-mass mixture material by using a granulator, wherein the diameter of the granules is 5mm, and drying the granules by hot air at 50 ℃ to obtain the coral artificial culture slow-release granular bait.
When feeding, 8: turning off the artificial light source at 00 hours, 8: turning off the wave raising equipment at 30 hours; meanwhile, the slow-release granular bait prepared by the invention is filled in a mesh bag at a ratio of 80 g/bag, and then the mesh bag is immersed in water and fixed at the position of 150-200 mm above the small coral spikes. The slow-release granular baits in the net bag are gradually dissolved, the baits can be observed to be slowly released and are preyed by coral tentacles, the net bag is removed after the slow-release duration lasts for 40 minutes, and the artificial light source and the wave raising equipment are started again after 30 minutes. At a rate of 0.2 m22 mesh bags are fixed in the area of the coral, and the residual bait with the bottom sinking is removed on the 2 nd day.
Example 2
1. Formula of slow-release granular bait for artificial coral culture
Mysidacea 500g
Mussel gonad 400g
Fish oil 5g
Ulva 200g
Kelp (250 g)
Chlorococcum minitans powder 250g
Yeast powder 40g
Rhodospirillum rubrum powder 70g
Vitamin 5g
MgS04 2g
Calcium oxide 2.0g
Carboxymethyl cellulose 30g
Chinese yam powder 25g
2. The preparation and feeding method of the coral artificial breeding slow-release bait comprises the following steps:
weighing Mysidacea, mussel gonad, Ulva lactuca and herba Zosterae Marinae according to the above formula proportion, adding appropriate amount of seawater, wet grinding into thick slurry, adding calcium oxide, fish oil, Chlorococcus microalga powder, yeast powder, Rhodospirillum rubrum powder, and vitamin MgS04The coral artificial culture slow-release granular bait is prepared by the steps of uniformly mixing carboxymethyl cellulose, yam powder raw materials and a proper amount of seawater into a wet-mass mixture, extruding and granulating the wet-mass mixture material by using a granulator, wherein the diameter of the granules is 30mm, and naturally airing or drying by hot air at 50 ℃ to obtain the coral artificial culture slow-release granular bait.
When feeding, 8: turning off the artificial light source at 00 hours, 8: turning off the wave raising equipment at 30 hours; meanwhile, the slow-release granular bait prepared by the invention is filled in a mesh bag at a ratio of 80 g/bag, and then the mesh bag is immersed in water and fixed at the position of 150-200 mm above the small coral spikes. The slow release granular bait in the mesh bag is gradually dissolved, the bait can be observed to be slowly released and is preyed by coral tentacles, when the slow release duration is 30 minutes, the mesh bag is removed, after 30 minutes,and re-starting the artificial light source and the wave raising equipment. At a rate of 0.2 m22 mesh bags are fixed in the area of the coral, and the residual bait with the bottom sinking is removed on the 2 nd day.
Claims (6)
1. A preparation method of slow-release granular bait for artificial coral culture is characterized by comprising the following steps:
1) formula of slow-release granular bait for artificial coral culture
300-500 g of mysid
Mussel gonad 400-500 g
5-8 g of fish oil
200-250 g ulva lactuca
200-250 g of kelp
200-250 g of nannochloropsis algae powder
40-60 g of yeast powder
50-100 g of rhodospirillum rubrum powder
5-7 g of vitamin
MgS04 1～2g
1.5-2.5 g of calcium oxide
25-30 g of carboxymethyl cellulose
25-30 g of yam powder;
2) the preparation method of the coral artificial breeding slow-release granular bait comprises the following steps:
weighing Mysidacea, mussel gonad, Ulva lactuca and herba Zosterae Marinae according to the above formula proportion, adding appropriate amount of seawater, wet grinding into thick slurry, adding calcium oxide, fish oil, Chlorococcus microalga powder, yeast powder, Rhodospirillum rubrum powder, vitamins, and MgS04The coral artificial culture slow-release granular bait is prepared by the steps of uniformly mixing carboxymethyl cellulose, yam powder and a proper amount of seawater into a wet-mass mixture, extruding and granulating the wet-mass mixture material by using a granulator, wherein the diameter of the granules is 30-50 mm, and naturally airing or drying by hot air at 50 ℃ to obtain the coral artificial culture slow-release granular bait.
2. The method for preparing the slow-release granular bait for artificial coral culture as claimed in claim 1, wherein the Rhodospirillum rubrum (A), (B) and (C) isRhodospirillum rubrum) The preparation method of the fungus powder comprises the following steps:
the 3L of seed culture solution comprises the following components in percentage by weight: 0.2% of ammonium chloride, 0.8% of sodium bicarbonate, 0.1% of dipotassium hydrogen phosphate, 0.40% of sodium acetate, 0.02% of magnesium sulfate and the balance of water; adjusting pH of the culture solution to 7.8, inoculating Rhodospirillum rubrum, performing static culture under natural light, centrifuging to precipitate thallus, and oven drying with hot air at 45-55 deg.C to obtain Rhodospirillum rubrum powder.
3. The preparation method of the coral artificial culture slow-release granular bait as claimed in claim 2, which is characterized in that: the static culture conditions were: the culture temperature is 30-32 ℃, and the culture time is 96-120 hours.
4. The preparation method of the slow-release granular bait for coral artificial breeding according to claim 1, wherein the preparation method of the nannochloropsis oculata powder comprises the following steps:
culturing the nannochloropsis oculata seeds, centrifuging to obtain precipitate, and drying with 45-55 deg.C hot air to obtain nannochloropsis oculata powder.
5. The preparation method of the coral artificial culture slow-release granular bait as claimed in claim 1, wherein the vitamins are compound vitamins, and the ratio of the vitamins is as follows: thiamine 26mg, riboflavin 105 mg, nicotinic acid 510 mg, calcium pantothenate 105 mg, vitamin B6105 mg of vitamin B120.26 mg, biotin 1.2 mg and folic acid 12 mg.
6. The preparation method of the coral artificial culture slow-release granule bait as claimed in claim 1, wherein the preparation method of the yam powder comprises the following steps: slicing rhizoma Dioscoreae, sun drying, pulverizing, and sieving with 200 mesh sieve to obtain rhizoma Dioscoreae powder.
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| CN107410757B (en) * | 2016-12-13 | 2021-06-25 | 福建师范大学 | Preparation method of composite liquid bait for artificial coral culture |
| CN112586630A (en) * | 2020-11-13 | 2021-04-02 | 海底世界(湖南)有限公司 | Coral compound bait |
| CN113243319B (en) * | 2021-05-21 | 2024-01-26 | 江苏绿岩生态技术股份有限公司 | Ecological restoration method and growth auxiliary system for coral reef |
| CN116439165B (en) * | 2023-05-04 | 2024-03-19 | 海南珊海海洋科技有限公司 | Breeding method for improving survival rate of coral larvae |
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| US20160192677A1 (en) * | 2014-12-04 | 2016-07-07 | Peter Saris | Method of treating coral, a coral food composition and a method of manufacture |
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