CN106577258A - Method for breeding special industrial high-resistance rape variety - Google Patents
Method for breeding special industrial high-resistance rape variety Download PDFInfo
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- CN106577258A CN106577258A CN201611141510.6A CN201611141510A CN106577258A CN 106577258 A CN106577258 A CN 106577258A CN 201611141510 A CN201611141510 A CN 201611141510A CN 106577258 A CN106577258 A CN 106577258A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention belongs to the technical field of special rape disease resistance breeding and discloses a method for breeding a special industrial high-resistance rape variety. The method includes that a high-erucic-acid rape variety, a glyphosate herbicide resistant rape variety, a sclerotinia sclerotiorum resistant variety, a rape virus disease resistant variety and a rape white rust resistant variety are taken as parents and subjected to hybridization, multi-cross polymerization and multi-generation resistance identification, erucic acid content measurement, agricultural and yield trait screening to obtain a special industrial high-resistance rape variety which is highly resistant to three diseases, resistant to glyphosate herbicide and high in erucic acid content. The special industrial high-resistance rape variety is resistant to diseases, planting the variety can reduce weeding labor cost and farm chemical consumption and increase yield, rape oil is high in erucic acid content, and erucic acid produced by taking rape oil as a raw material is quite extensive in industrial application.
Description
Technical field
Present invention relates particularly to a kind of selection of the special high anti-rape variety of industry, belongs to extraordinary Brassica campestris L breeding for disease resistance
Technical field.
Background technology
Organic Chemicals with oil, natural gas as representative, in chemical industry highly important effect has been played.But
As the mankind continually develop to earth mineral resources, the non-renewable resources such as petroleum and natural gas will be reduced constantly, eventually can
It is exhausted.After nineteen nineties, the developed country such as America and Europe carries out in the exploitation of " Green Lubricants and fuel oil "
Extensive research, the erucic acid with high erucic acid vegetable oil as raw material production, industrial use is quite varied, can be used for casting, machinery,
The numerous areas such as chemical industry, Aero-Space, its derived product erucic acid acyl ammonia is referred to as 21st century " kings of industrial chemicals ", and its is complete
Ball demand is just increased with annual 15% speed.The domestic and international market prospect of erucic acid and its derived product is very good.China
Staple vegetable oil content of erucic acid, well below the economic raw material standard of content 45%, causes erucic acid to produce mostly below 20%
Cost is greatly increased, and fine oiling enterprise is faced with serious raw material crisis.Breeding high-yield amount high erucic acid content it is industrial special
High erucic acid rape kind is very urgent.Brassica campestris L is susceptible to crop smothering, and underproduction 15%-20% is caused throughout the year, therefore, preventing and treating crop smothering is to oil
Dish production is of great significance.Main weeds have kind more than 20 in Rice-rape fields, cultivate and promote the rape variety of antiweed
Chemical weed control can be at any time carried out during Brassica campestris L is entirely given birth to, intertillage weeding recruitment is reduced, Weed infestation is reduced, Brassica campestris L is improved and is produced
Amount, increases benefit.Sclerotinia rot of colza, virosiss, white blister are that Brassica campestris L obtains three big Major Diseases, and China occurs most with the Yangtze river basin
Seriously, hypopathia year underproduction 10%-20%, the underproduction of severe disease year is up to 50% or so.More than one since century, both at home and abroad in Brassica campestris L disease
The aspect such as generation and preventing and treating has done substantial amounts of work, but fundamentally changes the situation of Brassica campestris L disease serious harm yet；Therefore,
Selection-breeding and increasingly it is subject to people's attention using disease-resistant variety.With developing rapidly for Chinese national economy, people's lives water
Flat continuous improvement, the consumption figure of edible oil grows with each passing day, and is rendered as the increasing situation of supply demand gap.In sum, build
The industrial Brassica campestris L high-efficient breeding technique system of high disease-resistant antiweed high erucic acid is found, is to promote agricultural sector structure adjustment, optimization
Pattern of farming, improves peasant planting benefit, alleviates the effective way of China's erucic acid and its derived product contradiction in short supply.
The content of the invention
It is an object of the invention to provide a kind of selection of the special high anti-rape variety of industry.
The present invention is with high erucic acid rape kind, resistance glyphosate herbicide rape variety, anti-sclerotinia rot of colza kind, oil resistant dish
Virosiss kind, anti-white rust of colza kind are parent, and by the polymerization that hybridizes, reestablishes diplomatic relations, and the Resistance Identification in Jing many generations, erucic acid contain
Fixed, agronomy and yield traits screening are measured, is bred as that simultaneously high 3 kinds of diseases, resistance glyphosate herbicide, works of high erucic acid content can be resisted
Industry tailored version rape variety, the Variety Disease-resistance, plant the kind can reduce weeding recruitment cost, reduce Pesticide use, improve produce
Amount, the vegetable oil content of erucic acid is high, makees the erucic acid of raw material production, and industrial use is quite varied.
To reach object above, the present invention is employed the following technical solutions：
(1) high erucic acid rape kind Mianyou No. 13 is selected to be parent P1（Yuan Daibin, etc. industrial special high erucic acid rape new varieties are continuous
The selection-breeding of oil 13. Chinese oil crop journal 2003,25 (3)：85-97）, resistance glyphosate rape variety Q3 is parent P2（Pu
Hui Ming, etc. the heredity and its application of Brassica campestris L herbicide resistance. Jiangsu's agriculture journal, 2003,19 (2):81-86）, oil resistant dish
Double No. 9 is parent P3 in sclerotiniose kind（Wang Hanzhong, etc. double No. 9 selection-breeding in anti-sclerotiniose double-low rapeseed new varieties. China
Oil crop journal, 2002,24 (4)：71-73）, oil resistant dish virosiss kind Lingot is parent P4（Liu Zhongsong. Brassica campestris L resists
Sick Advances of Studies In Heredity And Breeding. Chinese oil plant, 1989 (2):77-81）, anti-white rust of colza kind Regent or eastern spoke one
For parent P5（Liu Zhongsong. Brassica campestris L resistant heredity Research Advances on Breeding. Chinese oil plant, 1989 (2):77-81）Hybridized, with
P1 is female parent, makees hybridization P1/P2, P1/P3, P1/P4, P1/P5, obtains 4 hybridization F1, respectively with F1-P12、F1-P13、F1-P14、
F1- P15 is represented；
(2) F is planted1-P12、F1-P13、F1-P14、F1- P15 is each 10 plants, and the florescence makees hybridization P12/P13, P14/P15, must reestablish diplomatic relations
F1For seed F1-P123、F1-P145；
(3) F is planted1-P123、F1- P145, florescence bagging selfing, period of maturation individual plant sowing obtains F2For seed F2-P123、F2-
P145；
(4) F is planted respectively2For seed F2-P123、F2- P145, each combination plantation 350-400 strain, combines F2- P145 carries out disease
Viral disease, white blister Resistance Identification, filter out high anti-2 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique compared with
The big 30-40 bagging selfing of individual plant of many, seed, individual plant sowing；Combination F2- P123 seedling stages carry out antiweed glyphosate sieve
Choosing, removes not resistance glyphosate plant, and retaining resistance glyphosate plant simultaneously carries out sclerotinia rot of colza Resistance Identification, filter out it is high it is disease-resistant,
Tall and big compact, the effective branch of plant type is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, individual plant receive
Kind；By the F of upper choosing2-P123、F2- P145 individual plant sowing seeds carry out content of erucic acid measure, select list of the content of erucic acid more than 55%
Strain, obtains the high erucic acid F of choosing3For seed F3-P123、F3-P145；
(5) plantation step（4）Gained F3-P123、F3- P145, each combination plantation 320-350 strain, combines F3- P145 carries out disease
Viral disease, white blister Resistance Identification, filter out high anti-2 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique compared with
The big 30-35 bagging selfing of individual plant of many, seed, individual plant sowing；Combination F3- P123 seedling stages carry out antiweed glyphosate sieve
Choosing, removes not resistance glyphosate plant, and retaining resistance glyphosate plant simultaneously carries out sclerotinia rot of colza Resistance Identification, filter out it is high it is disease-resistant,
Tall and big compact, the effective branch of plant type is more, main inflorescence length, 30-35 bagging selfing of individual plant that silique is more, seed is big, individual plant receive
Kind；By the F of upper choosing3-P123、F3- P145 individual plant sowing seeds carry out content of erucic acid measure, select list of the content of erucic acid more than 55%
Strain, obtains the high erucic acid F of choosing4For seed F4-P123、F4-P145；
(6) plantation step（5）F obtained by screening4-P123、F4- P145, florescence respectively selects the excellent individual plant 3-5 of economical character
Strain, makees hybridization F4-P123/F4- P145, must reestablish diplomatic relations F1, with F1- P12345 is represented；
(7) F that reestablishes diplomatic relations is planted1- P12345 seeds, florescence bagging selfing, individual plant sowing obtains F2For seed F2-P12345；
(8) F is planted2- P12345 350-380 strains, seedling stage first carries out antiweed glyphosate screening, removes not resistance glyphosate
Individual plant, then Disease Resistance Identification is carried out, 3 resistant parent institute disease resistance species are identified, filter out simultaneously high anti-3 kinds of diseases, plant types
Tall and big compact, effective branch is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, period of maturation individual plant
Numbering sowing, the seed of results carries out content of erucic acid measure, selects individual plant of the content of erucic acid more than 55%, obtains the F of choosing3For seed
F3-P12345；
(9) plantation step（8）Screening gained F3- P12345 350-380 strains, seedling stage first carries out antiweed glyphosate screening, goes
Except the individual plant of not resistance glyphosate, then sclerotiniose, virosiss, white blister Resistance Identification are carried out, filter out and high resist 3 kinds of diseases, plant types
Tall and big compact, effective branch is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, period of maturation individual plant
Numbering sowing, the seed of results carries out content of erucic acid measure, selects the content of erucic acid individual plant 5-8 more than 55%, obtains the F of choosing4Generation
Seed F4-P12345；
(10) strain plantation step（9）Screening gained F4- P12345,180 plants of each strain, it is sweet that seedling stage first carries out antiweed grass
Phosphine is screened, and removes the strain of not resistance glyphosate, then carries out Disease Resistance Identification, filters out simultaneously high anti-3 kinds of diseases, plant type tall and big tight
Gather, effective branch is more, main inflorescence length, 20 bagging selfings of individual plant that silique is more, seed is big, period of maturation individual plant numbering sowing,
The seed of results carries out content of erucic acid measure, selects individual plant 5 of the content of erucic acid more than 55%, obtains the F of choosing5For seed F5-
P12345；
(11) strain plantation step（10）F obtained by screening5- P12345,100 plants of each strain, seedling stage first carries out antiweed
Glyphosate is screened, and removes the strain of not resistance glyphosate, then carries out Disease Resistance Identification, filters out simultaneously high anti-3 kinds of diseases, plant type high
Big compact, effective branch is more, main inflorescence length, 20 bagging selfings of individual plant that silique is more, seed is big, period of maturation individual plant numbering receipts
Kind, the seed of results carries out content of erucic acid measure, selects individual plant 5 of the content of erucic acid more than 55%, obtains the F of choosing6For seed F6-
P12345；
(12) strain plantation step（11）5 F obtained by screening6- P12345,80 plants of each strain, the period of maturation is to period of duration, strain
Height, plant type, effective branch, main inflorescence length, silique number, seed size are screened, and select 1 mixing of strain that Comprehensive Traits are excellent
Sowing, that is, be bred as high yield, high erucic acid, anti-sclerotinia rot of colza, Rape virus disease, the industrial special high oil resistant vegetable of white rust of colza
Kind.
Beneficial effects of the present invention：
Effect of the invention is that respectively resistance glyphosate herbicide, high erucic acid, anti-sclerotinia rot of colza, Rape virus disease, Brassica campestris L
White rust ospc gene by hybridization, breeding method of reestablishing diplomatic relations polymerization import high yield rape variety in, be bred as agronomy and yield traits it is excellent,
High yield, disease-resistant, antiweed high erucic acid rape kind.The rape variety better resistance, can reduce Weed infestation and weeding
Work, can improve yield of rape, increase peasant economy benefit, and per mu increases yield 15-20% than ordinary cole kind, reduces recruitment
Cost 80-100 is first, reduces dispenser cost 100-120 unit, and per mu can increase economic benefit 380-440 unit, while reducing pesticide
Pollution to environment.
Specific embodiment
Embodiment 1：
(1) high erucic acid rape kind Mianyou No. 13 is selected（P1）, resistance glyphosate herbicide rape variety Q3（P2）, anti-sclerotinia rot of colza
It is double No. 9 in kind（P3）, oil resistant dish virosiss kind Lingot（P4）, anti-white rust of colza kind Regent（P5）For hybridization
Parent, with P1 as female parent, makees hybridization P1/P2, P1/P3, P1/P4, P1/P5, obtains 4 hybridization F1, respectively with F1-P12、F1-P13、
F1-P14、F1- P15 is represented；
(2) F is planted1-P12、F1-P13、F1-P14、F1- P15 is each 10 plants, and the florescence makees hybridization P12/P13, P14/P15, must reestablish diplomatic relations
F1For seed F1-P123、F1-P145；
(3) F is planted1-P123、F1- P145, florescence bagging selfing, period of maturation individual plant sowing obtains F2For seed F2-P123、F2-
P145；
(4) F is planted respectively2For seed F2-P123、F2- P145, each combination plantation 350-400 strain, combines F2- P145 carries out disease
Viral disease, white blister Resistance Identification, filter out high anti-2 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique compared with
The big 30-40 bagging selfing of individual plant of many, seed, individual plant sowing；Combination F2- P123 seedling stages carry out antiweed glyphosate sieve
Choosing, removes not resistance glyphosate plant, and retaining resistance glyphosate plant simultaneously carries out sclerotinia rot of colza Resistance Identification, filter out it is high it is disease-resistant,
Tall and big compact, the effective branch of plant type is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, individual plant receive
Kind；By the F of upper choosing2-P123、F2- P145 individual plant sowing seeds carry out content of erucic acid measure, select list of the content of erucic acid more than 55%
Strain, obtains the high erucic acid F of choosing3For seed F3-P123、F3-P145；
(5) plantation step（4）Gained F3-P123、F3- P145, each combination plantation 320-350 strain, combines F3- P145 carries out disease
Viral disease, white blister Resistance Identification, filter out high anti-2 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique compared with
The big 30-35 bagging selfing of individual plant of many, seed, individual plant sowing；Combination F3- P123 seedling stages carry out antiweed glyphosate sieve
Choosing, removes not resistance glyphosate plant, and retaining resistance glyphosate plant simultaneously carries out sclerotinia rot of colza Resistance Identification, filter out it is high it is disease-resistant,
Tall and big compact, the effective branch of plant type is more, main inflorescence length, 30-35 bagging selfing of individual plant that silique is more, seed is big, individual plant receive
Kind；By the F of upper choosing3-P123、F3- P145 individual plant sowing seeds carry out content of erucic acid measure, select list of the content of erucic acid more than 55%
Strain, obtains the high erucic acid F of choosing4For seed F4-P123、F4-P145；
(6) plantation step（5）F obtained by screening4-P123、F4- P145, florescence respectively selects the excellent individual plant 3-5 of economical character
Strain, makees hybridization F4-P123/F4- P145, must reestablish diplomatic relations F1, with F1- P12345 is represented；
(7) F that reestablishes diplomatic relations is planted1- P12345 seeds, florescence bagging selfing, individual plant sowing obtains F2For seed F2-P12345；
(8) F is planted2- P12345 350-380 strains, seedling stage first carries out antiweed glyphosate screening, removes not resistance glyphosate
Individual plant, then Disease Resistance Identification is carried out, 3 resistant parent institute disease resistance species are identified, filter out simultaneously high anti-3 kinds of diseases, plant types
Tall and big compact, effective branch is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, period of maturation individual plant
Numbering sowing, the seed of results carries out content of erucic acid measure, selects individual plant of the content of erucic acid more than 55%, obtains the F of choosing3For seed
F3-P12345；
(9) plantation step（8）Screening gained F3- P12345 350-380 strains, seedling stage first carries out antiweed glyphosate screening, goes
Except the individual plant of not resistance glyphosate, then sclerotiniose, virosiss, white blister Resistance Identification are carried out, filter out and high resist 3 kinds of diseases, plant types
Tall and big compact, effective branch is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, period of maturation individual plant
Numbering sowing, the seed of results carries out content of erucic acid measure, selects the content of erucic acid individual plant 5-8 more than 55%, obtains the F of choosing4Generation
Seed F4-P12345；
(10) strain plantation step（9）Screening gained F4- P12345,180 plants of each strain, it is sweet that seedling stage first carries out antiweed grass
Phosphine is screened, and removes the strain of not resistance glyphosate, then carries out Disease Resistance Identification, filters out simultaneously high anti-3 kinds of diseases, plant type tall and big tight
Gather, effective branch is more, main inflorescence length, 20 bagging selfings of individual plant that silique is more, seed is big, period of maturation individual plant numbering sowing,
The seed of results carries out content of erucic acid measure, selects individual plant 5 of the content of erucic acid more than 55%, obtains the F of choosing5For seed F5-
P12345；
(11) strain plantation step（10）F obtained by screening5- P12345,100 plants of each strain, seedling stage first carries out antiweed
Glyphosate is screened, and removes the strain of not resistance glyphosate, then carries out Disease Resistance Identification, filters out simultaneously high anti-3 kinds of diseases, plant type high
Big compact, effective branch is more, main inflorescence length, 20 bagging selfings of individual plant that silique is more, seed is big, period of maturation individual plant numbering receipts
Kind, the seed of results carries out content of erucic acid measure, selects individual plant 5 of the content of erucic acid more than 55%, obtains the F of choosing6For seed F6-
P12345；
(12) strain plantation step（11）5 F obtained by screening6- P12345,80 plants of each strain, the period of maturation is to period of duration, strain
Height, plant type, effective branch, main inflorescence length, silique number, seed size are screened, and select 1 mixing of strain that Comprehensive Traits are excellent
Sowing, that is, be bred as high yield, high erucic acid, anti-sclerotinia rot of colza, Rape virus disease, the industrial special high oil resistant vegetable of white rust of colza
Plant " work oil is anti-No. 1 ".
" work oil is anti-No. 1 " yield is high, the kghm of average yield per mu oil mass 2817.1-2, than compareing Mianyou No. 13
（2430.3kg·hm-2）Increase by 15.9%, reduce 80 yuan of recruitment cost, reduce 100 yuan of dispenser cost, per mu can increase economy
380 yuan or so of benefit, while reducing pollution of the pesticide to environment.
Embodiment 2：
(1) high erucic acid rape kind Mianyou No. 13 is selected（P1）, resistance glyphosate herbicide rape variety Q3（P2）, anti-sclerotinia rot of colza
It is double No. 9 in kind（P3）, oil resistant dish virosiss kind Lingot（P4）, anti-white rust of colza kind east spoke one（P5）For miscellaneous
Parent is handed over, with P1 as female parent, makees hybridization P1/P2, P1/P3, P1/P4, P1/P5, obtain 4 hybridization F1, respectively with F1-P12、F1-
P13、F1-P14、F1- P15 is represented；
(2) F is planted1-P12、F1-P13、F1-P14、F1- P15 is each 10 plants, and the florescence makees hybridization P12/P13, P14/P15, must reestablish diplomatic relations
F1For seed F1-P123、F1-P145；
(3) F is planted1-P123、F1- P145, florescence bagging selfing, period of maturation individual plant sowing obtains F2For seed F2-P123、F2-
P145；
(4) F is planted respectively2For seed F2-P123、F2- P145, each combination plantation 350-400 strain, combines F2- P145 carries out disease
Viral disease, white blister Resistance Identification, filter out high anti-2 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique compared with
The big 30-40 bagging selfing of individual plant of many, seed, individual plant sowing；Combination F2- P123 seedling stages carry out antiweed glyphosate sieve
Choosing, removes not resistance glyphosate plant, and retaining resistance glyphosate plant simultaneously carries out sclerotinia rot of colza Resistance Identification, filter out it is high it is disease-resistant,
Tall and big compact, the effective branch of plant type is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, individual plant receive
Kind；By the F of upper choosing2-P123、F2- P145 individual plant sowing seeds carry out content of erucic acid measure, select list of the content of erucic acid more than 55%
Strain, obtains the high erucic acid F of choosing3For seed F3-P123、F3-P145；
(5) plantation step（4）Gained F3-P123、F3- P145, each combination plantation 320-350 strain, combines F3- P145 carries out disease
Viral disease, white blister Resistance Identification, filter out high anti-2 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique compared with
The big 30-35 bagging selfing of individual plant of many, seed, individual plant sowing；Combination F3- P123 seedling stages carry out antiweed glyphosate sieve
Choosing, removes not resistance glyphosate plant, and retaining resistance glyphosate plant simultaneously carries out sclerotinia rot of colza Resistance Identification, filter out it is high it is disease-resistant,
Tall and big compact, the effective branch of plant type is more, main inflorescence length, 30-35 bagging selfing of individual plant that silique is more, seed is big, individual plant receive
Kind；By the F of upper choosing3-P123、F3- P145 individual plant sowing seeds carry out content of erucic acid measure, select list of the content of erucic acid more than 55%
Strain, obtains the high erucic acid F of choosing4For seed F4-P123、F4-P145；
(6) plantation step（5）F obtained by screening4-P123、F4- P145, florescence respectively selects the excellent individual plant 3-5 of economical character
Strain, makees hybridization F4-P123/F4- P145, must reestablish diplomatic relations F1, with F1- P12345 is represented；
(7) F that reestablishes diplomatic relations is planted1- P12345 seeds, florescence bagging selfing, individual plant sowing obtains F2For seed F2-P12345；
(8) F is planted2- P12345 350-380 strains, seedling stage first carries out antiweed glyphosate screening, removes not resistance glyphosate
Individual plant, then Disease Resistance Identification is carried out, 3 resistant parent institute disease resistance species are identified, filter out simultaneously high anti-3 kinds of diseases, plant types
Tall and big compact, effective branch is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, period of maturation individual plant
Numbering sowing, the seed of results carries out content of erucic acid measure, selects individual plant of the content of erucic acid more than 55%, obtains the F of choosing3For seed
F3-P12345；
(9) plantation step（8）Screening gained F3- P12345 350-380 strains, seedling stage first carries out antiweed glyphosate screening, goes
Except the individual plant of not resistance glyphosate, then sclerotiniose, virosiss, white blister Resistance Identification are carried out, filter out and high resist 3 kinds of diseases, plant types
Tall and big compact, effective branch is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, period of maturation individual plant
Numbering sowing, the seed of results carries out content of erucic acid measure, selects the content of erucic acid individual plant 5-8 more than 55%, obtains the F of choosing4Generation
Seed F4-P12345；
(10) strain plantation step（9）Screening gained F4- P12345,180 plants of each strain, it is sweet that seedling stage first carries out antiweed grass
Phosphine is screened, and removes the strain of not resistance glyphosate, then carries out Disease Resistance Identification, filters out simultaneously high anti-3 kinds of diseases, plant type tall and big tight
Gather, effective branch is more, main inflorescence length, 20 bagging selfings of individual plant that silique is more, seed is big, period of maturation individual plant numbering sowing,
The seed of results carries out content of erucic acid measure, selects individual plant 5 of the content of erucic acid more than 55%, obtains the F of choosing5For seed F5-
P12345；
(11) strain plantation step（10）F obtained by screening5- P12345,100 plants of each strain, seedling stage first carries out antiweed
Glyphosate is screened, and removes the strain of not resistance glyphosate, then carries out Disease Resistance Identification, filters out simultaneously high anti-3 kinds of diseases, plant type high
Big compact, effective branch is more, main inflorescence length, 20 bagging selfings of individual plant that silique is more, seed is big, period of maturation individual plant numbering receipts
Kind, the seed of results carries out content of erucic acid measure, selects individual plant 5 of the content of erucic acid more than 55%, obtains the F of choosing6For seed F6-
P12345；
(12) strain plantation step（11）5 F obtained by screening6- P12345,80 plants of each strain, the period of maturation is to period of duration, strain
Height, plant type, effective branch, main inflorescence length, silique number, seed size are screened, and select 1 mixing of strain that Comprehensive Traits are excellent
Sowing, that is, be bred as high yield, high erucic acid, anti-sclerotinia rot of colza, Rape virus disease, the industrial special high oil resistant vegetable of white rust of colza
Plant " work oil is anti-No. 2 ".
" work oil is anti-No. 2 " yield is high, the kghm of average yield per mu oil mass 3036.1-2, than compareing Mianyou No. 13
（2430.3kg·hm-2）Increase by 24.9%, reduce 80 yuan of recruitment cost, reduce 100 yuan of dispenser cost, per mu can increase economy
440 yuan of benefit, while reducing pollution of the pesticide to environment.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (1)
1. the selection of the special high anti-rape variety of a kind of industry, it is characterised in that the selection include following methods with
Step：
（1）High erucic acid rape kind Mianyou No. 13 is selected for parent P1, resistance glyphosate herbicide rape variety Q3 is parent P2, oil resistant
Double No. 9 is parent P3 in dish sclerotiniose kind, and oil resistant dish virosiss kind Lingot is parent P4, anti-white rust of colza kind
Regent or eastern spokes one are hybridized for parent P5, with P1 as female parent, make hybridization P1/P2, P1/P3, P1/P4, P1/P5, obtain 4
Individual hybridization F1, respectively with F1-P12、F1-P13、F1-P14、F1- P15 is represented；
（2）Plantation F1-P12、F1-P13、F1-P14、F1- P15 is each 10 plants, and the florescence makees hybridization P12/P13, P14/P15, and must reestablish diplomatic relations F1
For seed F1-P123、F1-P145；
（3）Plantation F1-P123、F1- P145, florescence bagging selfing, period of maturation individual plant sowing obtains F2For seed F2-P123、F2-
P145；
（4）F is planted respectively2For seed F2-P123、F2- P145, each combination plantation 350-400 strain, combines F2- P145 carries out disease
Viral disease, white blister Resistance Identification, filter out high anti-2 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique compared with
The big 30-40 bagging selfing of individual plant of many, seed, individual plant sowing；Combination F2- P123 seedling stages carry out antiweed glyphosate sieve
Choosing, removes not resistance glyphosate plant, and retaining resistance glyphosate plant simultaneously carries out sclerotinia rot of colza Resistance Identification, filter out it is high it is disease-resistant,
Tall and big compact, the effective branch of plant type is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, individual plant receive
Kind；By the F of upper choosing2-P123、F2- P145 individual plant sowing seeds carry out content of erucic acid measure, select list of the content of erucic acid more than 55%
Strain, obtains the high erucic acid F of choosing3For seed F3-P123、F3-P145；
（5）Plantation step（4）Gained F3-P123、F3- P145, each combination plantation 320-350 strain, combines F3- P145 carries out disease
Viral disease, white blister Resistance Identification, filter out high anti-2 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique compared with
The big 30-35 bagging selfing of individual plant of many, seed, individual plant sowing；Combination F3- P123 seedling stages carry out antiweed glyphosate sieve
Choosing, removes not resistance glyphosate plant, and retaining resistance glyphosate plant simultaneously carries out sclerotinia rot of colza Resistance Identification, filter out it is high it is disease-resistant,
Tall and big compact, the effective branch of plant type is more, main inflorescence length, 30-35 bagging selfing of individual plant that silique is more, seed is big, individual plant receive
Kind；By the F of upper choosing3-P123、F3- P145 individual plant sowing seeds carry out content of erucic acid measure, select list of the content of erucic acid more than 55%
Strain, obtains the high erucic acid F of choosing4For seed F4-P123、F4-P145；
（6）Plantation step（5）F obtained by screening4-P123、F4- P145, florescence respectively selects the excellent individual plant 3-5 strains of economical character,
Make hybridization F4-P123/F4- P145, must reestablish diplomatic relations F1, with F1- P12345 is represented；
（7）Plant the F that reestablishes diplomatic relations1- P12345 seeds, florescence bagging selfing, individual plant sowing obtains F2For seed F2-P12345；
（8）Plantation F2- P12345 350-380 strains, seedling stage first carries out antiweed glyphosate screening, removes the list of not resistance glyphosate
Strain, then Disease Resistance Identification is carried out, 3 resistant parent institute disease resistance species are identified, filter out simultaneously high anti-3 kinds of diseases, plant type high
Big compact, effective branch is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, period of maturation individual plant volume
Number sowing, the seed of results carries out content of erucic acid measure, selects individual plant of the content of erucic acid more than 55%, obtains the F of choosing3For seed F3-
P12345；
（9）Plantation step（8）Screening gained F3- P12345 350-380 strains, seedling stage first carries out antiweed glyphosate screening, goes
Except the individual plant of not resistance glyphosate, then sclerotiniose, virosiss, white blister Resistance Identification are carried out, filter out and high resist 3 kinds of diseases, plant types
Tall and big compact, effective branch is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, period of maturation individual plant
Numbering sowing, the seed of results carries out content of erucic acid measure, selects the content of erucic acid individual plant 5-8 more than 55%, obtains the F of choosing4Generation
Seed F4-P12345；
（10）Strain plantation step（9）Screening gained F4- P12345,180 plants of each strain, it is sweet that seedling stage first carries out antiweed grass
Phosphine is screened, and removes the strain of not resistance glyphosate, then carries out Disease Resistance Identification, filters out simultaneously high anti-3 kinds of diseases, plant type tall and big tight
Gather, effective branch is more, main inflorescence length, 20 bagging selfings of individual plant that silique is more, seed is big, period of maturation individual plant numbering sowing,
The seed of results carries out content of erucic acid measure, selects individual plant 5 of the content of erucic acid more than 55%, obtains the F of choosing5For seed F5-
P12345；
（11）Strain plantation step（10）F obtained by screening5- P12345,100 plants of each strain, seedling stage first carries out antiweed grass
Sweet phosphine screening, removes the strain of not resistance glyphosate, then carries out Disease Resistance Identification, filters out simultaneously high anti-3 kinds of diseases, plant types tall and big
Compact, effective branch is more, main inflorescence length, 20 bagging selfings of individual plant that silique is more, seed is big, period of maturation individual plant numbering receive
Kind, the seed of results carries out content of erucic acid measure, selects individual plant 5 of the content of erucic acid more than 55%, obtains the F of choosing6For seed F6-
P12345；
（12）Strain plantation step（11）5 F obtained by screening6- P12345,80 plants of each strain, the period of maturation is to period of duration, strain
Height, plant type, effective branch, main inflorescence length, silique number, seed size are screened, and select 1 mixing of strain that Comprehensive Traits are excellent
Sowing, that is, be bred as high yield, high erucic acid, anti-sclerotinia rot of colza, Rape virus disease, the industrial special high oil resistant vegetable of white rust of colza
Kind.
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| CN109197428A (en) * | 2018-07-23 | 2019-01-15 | 华中农业大学 | A method of susceptible variety disease incidence is reduced with anti-clubroot rape variety mixed seeding |
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