CN106577257A - High-resistance double-low yellow-seeded rape variety breeding method - Google Patents
High-resistance double-low yellow-seeded rape variety breeding method Download PDFInfo
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- CN106577257A CN106577257A CN201611139675.XA CN201611139675A CN106577257A CN 106577257 A CN106577257 A CN 106577257A CN 201611139675 A CN201611139675 A CN 201611139675A CN 106577257 A CN106577257 A CN 106577257A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention belongs to the technical field of rape disease resistance breeding and quality breeding and discloses a high-resistance double-low yellow-seeded rape variety breeding method. The method includes that a yellow-seeded brassica napus variety, a high-oleic-acid variety, a low-erucic-acid variety, a low-glucosinolate variety, a sclerotinia sclerotiorum resistant variety, a rape virus disease resistant variety and a rape white rust resistant variety are taken as parents and subjected to hybridization, multi-cross polymerization and multi-generation resistance identification and quality screening to obtain a high-resistance double-low yellow-seeded rape variety which is highly resistant to three diseases, high in oleic acid content and low in erucic acid and glucosinolate content. The high-resistance double-low yellow-seeded rape variety is high in quality and disease resistance, consumption of farm chemicals can be reduced by planting of the variety, environment pollution is abated while yield is increased, and income of peasants can be increased.
Description
Technical field
Present invention relates particularly to a kind of selection of high anti-double low yellow seed rape kinds, belongs to Brassica campestris L breeding for disease resistance and product
Matter breeding technical field.
Background technology
China is rapeseed product big country of the world, and area and total output occupy first place in the world, and Semen Brassicae campestriss are that China is edible plants
The main source of thing oil.The oil content of yellow-seeded brassica napus is generally higher than black seed rape, and yellow seed rape has kind of a thin skin, pigment
Content is few, cellulose and polyphenol content are low, oily as clear as crystal, the series of advantages such as protein content height, can be notable
Improve the economic benefit of grower and processing enterprise.Oleic acid is the important composition of canola fatty acids, there is higher nutritive value, heat
Stability is preferable.The oleic acid content of high oleic acid vegetable oil is high, and linoleic acid, linolenic acid content are low, it is possible to decrease the cardiovascular of Overweight people
Low-density lipoprotein cholesterol content in disease and blood plasma, effective prevention of arterial hardening, is difficult at high temperature oxidation deterioration,
Long shelf-life, does not smolder when higher temperature is heated to, and cooking time is reduced, and loss is also less, and deep fried product also possesses excellent
Fragrance.Therefore, the research of high oleic acid Brassica campestris L is subject to highest attention both domestic and external.Double-low rapeseed refers to low erucic acid, low-sulfur salidroside content
Rape variety, national standard be in oil product content of erucic acid be less than 5%, sulphur resources should be less than 30 micromoles per gram cakes in grouts.
Meet the two standards can be described as quality rape substantially.Erucic acid relation oil quality, the lower oil quality of erucic acid is better.Vegetable oil
Nutritive value depend on wherein various content of fatty acid such as Oleic acid, linoleic acid, erucic acid, linolenic acid.Sulfur glycoside is in Semen Brassicae campestris
Main harmful components, itself is simultaneously nontoxic, but noxious substance can be generated in the presence of enzyme.The high-sulfur glycoside rape cake dregs of rice feed hen,
Not only body constitution declines, egg production is reduced, and pneumorrhagia complication occurs, and causes mortality rate to be up to 62%-88%.Sclerotinia rot of colza, disease
Viral disease, white blister are that Brassica campestris L obtains three big Major Diseases, and China occurs most serious, hypopathia year underproduction 10%-20%, weight with the Yangtze river basin
The sick year underproduction is up to 50% or so.More than one since century, both at home and abroad the aspect such as generation and preventing and treating in Brassica campestris L disease has done substantial amounts of
Work, but fundamentally change the situation of Brassica campestris L disease serious harm yet, therefore, selection-breeding and increasingly received using disease-resistant variety
To the attention of people.With developing rapidly for Chinese national economy, the continuous improvement of living standards of the people, the consumption figure of edible oil
Grow with each passing day, be rendered as the increasing situation of supply demand gap.Therefore, set up high disease-resistant high oleic acid content double-low rapeseed efficiently to educate
Technical system is planted, is relied on scientific and technological advances, constantly release and convert good quality and high output new varieties, be to promote agricultural sector structure adjustment,
Optimization edible oil quality, improves peasant planting benefit, alleviates the effective way of China's edible oil contradiction in short supply.
The content of the invention
It is an object of the invention to provide a kind of selection of high anti-double low yellow seed rape kinds, so as to greatly improve Brassica campestris L product
The disease resistance planted and oil quality.
The present invention is with yellow-seeded brassica napus kind, high oleic acid kind, low erucic acid kind, low sulfatide kind, anti-Sclerotina Sclerotiorum in Winter Rape
Core disease kind, oil resistant dish virosiss kind, anti-white rust of colza kind are parent, by the polymerization that hybridizes, reestablishes diplomatic relations, and Jing many generations
Resistance Identification, quality-screening, are bred as grain characters and resist 3 kinds of diseases, high oleic acid, low erucic acid, low sulfatides to contain for yellow seed, energy are simultaneously high
The anti-double low yellow seed rape kinds of the height of amount, the variety and quality is good, disease resistance is good, and planting the kind can reduce the usage amount of pesticide,
Environmental pollution is reduced while improving yield, is increased farmers' income.
To reach object above, the present invention is employed the following technical solutions：
(1) yellow-seeded brassica napus kind YR5602 is selected（P1）（Zhang Ruimao, etc. Wild cabbage type true yellow seed rape new lines YR5602
Selection-breeding. seed, 2007,26（4）：87-90）, cabbage type rape high oleic acid kind FU003, FU012（P2）（Take reform, etc.
The reciprocal crossess genetic research of cabbage type rape high oleic acid material. Agriculture of Anhui science, 2007,35 (7):1927-1929.Wild cabbage type
Rape high oleic acid material FU003, FU012 oleic acid contents are up to more than 80%）, low erucic acid, low sulfatide kind Zhejiang University 619（P3）（Perhaps
The tinkling of pieces of jade. the selection-breeding and high-yield culture technique research of " double high double low " Brassica campestris L disease-resistant varieties Zhejiang University 619. nuclear agricultural science report, 2013,27 (
12) : 1839 - 1844）, it is double No. 9 in anti-sclerotinia rot of colza kind（P4）（Wang Hanzhong, etc. anti-sclerotiniose double-low rapeseed new product
Double No. 9 selection-breeding in kind. Chinese oil crop journal, 2002,24 (4)：71-73）, oil resistant dish virosiss kind Lingot
（P5）（Liu Zhongsong. Brassica campestris L resistant heredity Research Advances on Breeding. Chinese oil plant, 1989 (2):77-81）, anti-white rust of colza product
Plant Regent, eastern spoke one（P6）（Liu Zhongsong. Brassica campestris L resistant heredity Research Advances on Breeding. Chinese oil plant, 1989 (2):77-
81）For hybrid strain, make maternal with P1, make hybridization P1/P2, P1/P3, make maternal with P4, make hybridization P4/P5, make hybridization P4/
P6, obtains 4 hybridization F1, respectively with F1-P12、F1-P13、F1-P45、F1- P46 is represented；
(2) F is planted1-P12、F1-P13、F1-P45、F1- P46 is each 10 plants, and the florescence makees hybridization P12/P13, P45/P46, must reestablish diplomatic relations
F1For seed F1-P123、F1-P456；
(3) F is planted1-P123、F1- P456, florescence bagging selfing, period of maturation individual plant sowing obtains F2For seed F2-P123、F2-
P456；
(4) F is planted respectively2For seed F2-P123、F2- P456, each combination plantation 150-200 strain, combines F2- P456 carries out bacterium
Core disease, virosiss, white blister Resistance Identification, filter out that high anti-3 kinds of diseases, tall and big compact, the effective branch of plant type be more, main inflorescence
Long, 10 bagging selfings of individual plant that silique is more, seed is big, individual plant sowing obtains F3For seed F3-P456；Combination F2- P123 baggings
Selfing, selects the seed of performance yellow grain characters in the seed of results, obtain the F of choosing3For seed F3-P123；
(5) F is planted3-P123、F3- P456, each combination plantation 150-200 strain, combines F3- P456 carry out sclerotiniose, virosiss,
White blister Resistance Identification, filters out that high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 10 bagging selfings of individual plant of grain, individual plant sowing obtains F4For seed F4-P456；Combination F3- P123 bagging selfings, the kind of results
The unseparated individual plant of performance yellow grain characters is selected in son, Oleic acid, erucic acid, glucosinolate content is carried out respectively and is determined, screened fuel-displaced
Acid content more than 80%, content of erucic acid is less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 5, obtain the F of choosing4In generation, plants
Sub- F4-P123；
(6) plant（5）F obtained by screening4-P123、F4- P456, florescence respectively selects 3 plants excellent of individual plant of economical character, makees miscellaneous
Hand over F4-P123/F4- P456, must reestablish diplomatic relations F1, with F1- P123456 is represented；
(7) F is planted1- P123456 seeds, florescence bagging selfing, individual plant sowing obtains F2For seed F2-P123456；
(8) F is planted2- P123456 320-380 strains, and Resistance Identification is carried out, identify 3 resistant parent institute disease resistance species, sieve
Select simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, individual plant 30- that silique is more, seed is big
40 bagging selfings, period of maturation individual plant numbering sowing selects the seed of performance yellow grain characters in the seed of results, obtain choosing
F3For seed F3-P123456；
(9) F is planted3- P123456 250-300 strains, carry out sclerotiniose, virosiss, white blister Resistance Identification, filter out high by anti-3
Disease, many tall and big compact, effective branches of plant type, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big are planted,
Period of maturation individual plant numbering sowing, selects the performance unseparated individual plant of yellow grain characters in the seed of results, carry out respectively Oleic acid,
Erucic acid, glucosinolate content are determined, and are filtered out oleic acid content more than 80%, content of erucic acid and are less than 20 μ less than 0.5%, glucosinolate content
mol.g-1Individual plant 5, obtain the F of choosing4For seed F4-P123456；
(10) strain plantation F4- P123456,180 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institute disease resistances
Species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed is big
20-25 bagging selfing of individual plant, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, seed
Oleic acid, erucic acid, glucosinolate content are carried out respectively to determine, and are filtered out oleic acid content more than 80%, content of erucic acid and are contained less than 0.5%, sulfur glycoside
Amount is less than 20 μm of ol.g-1Individual plant 5, obtain the F of choosing5For seed F5-P123456；
(11) F selected in strain plantation5- P123456,100 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institutes
Disease resistance species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 20 bagging selfings of individual plant of grain, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, plants
Son carries out respectively Oleic acid, erucic acid, glucosinolate content and determines, and filters out oleic acid content more than 80%, content of erucic acid less than 0.5%, sulfur glycoside
Content is less than 20 μm of ol.g-1Individual plant 5, obtain the F of choosing6For seed F6-P123456；
(12) F selected in strain plantation6- P123456,80 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institutes
Disease resistance species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 10 bagging selfings of individual plant of grain, period of maturation individual plant numbering sowing, seed carries out respectively Oleic acid, erucic acid, glucosinolate content and determines,
Filter out oleic acid content more than 80%, content of erucic acid and be less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 2-3, that is, educate
Into high yield, yellow seed, high oleic acid, low erucic acid, low sulfatide, anti-sclerotinia rot of colza, Rape virus disease, white rust of colza Gao Kangshuan
Low yellow seed rape kind.
Beneficial effects of the present invention：
Effect of the invention is that respectively yellow seed, high oleic acid, low erucic acid, low sulfatide, anti-sclerotinia rot of colza, Rape virus
Disease, white rust of colza gene are imported in high yield rape variety by hybridization, breeding method of reestablishing diplomatic relations polymerization, are bred as agronomy and yield
Shape is excellent, the high-quality rape variety of yellow seed, disease-resistant, high oleic acid, low erucic acid, low sulfatide.The anti-double low yellow seed rape product of the height
Yield height, good quality, better resistance are planted, per mu increases yield 14-22% than ordinary cole kind, reduces dispenser cost 100-120
Unit, Semen Brassicae campestriss purchasing price increases 26-30%, and per mu can increase economic benefit 360-420 unit, while reducing pesticide to environment
Pollution.
Specific embodiment
Embodiment 1：
(1) yellow-seeded brassica napus kind YR5602 is selected（P1）, rape high oleic acid kind FU003（P2）, low erucic acid, low sulfatide product
Plant Zhejiang University 619（P3）, it is double No. 9 in anti-sclerotinia rot of colza kind（P4）, oil resistant dish virosiss kind Lingot（P5）, oil resistant dish
White blister kind Regent（P6）For hybrid strain, make maternal with P1, make hybridization P1/P2, P1/P3, make maternal with P4, make to hybridize
P4/P5, make hybridization P4/P6, obtain 4 hybridization F1, respectively with F1-P12、F1-P13、F1-P45、F1- P46 is represented；
(2) F is planted1-P12、F1-P13、F1-P45、F1- P46 is each 10 plants, and the florescence makees hybridization P12/P13, P45/P46, must reestablish diplomatic relations
F1For seed F1-P123、F1-P456；
(3) F is planted1-P123、F1- P456, florescence bagging selfing, period of maturation individual plant sowing obtains F2For seed F2-P123、F2-
P456；
(4) F is planted respectively2For seed F2-P123、F2- P456, each 150 plants of combination plantation, combines F2- P456 carries out sclerotium
Disease, virosiss, white blister Resistance Identification, filter out high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length,
10 bagging selfings of individual plant that silique is more, seed is big, individual plant sowing obtains F3For seed F3-P456；Combination F2- P123 baggings are certainly
Hand over, the seed of performance yellow grain characters is selected in the seed of results, obtain the F of choosing3For seed F3-P123；
(5) F is planted3-P123、F3- P456, each 150 plants of combination plantation, combines F3- P456 carries out sclerotiniose, virosiss, white rust
Sick Resistance Identification, filters out that high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed is big
10 bagging selfings of individual plant, individual plant sowing obtains F4For seed F4-P456；Combination F3- P123 bagging selfings, in the seed of results
The unseparated individual plant of performance yellow grain characters is selected, Oleic acid, erucic acid, glucosinolate content is carried out respectively and is determined, filtered out Oleic acid and contain
Amount more than 80%, content of erucic acid is less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 5, obtain the F of choosing4For seed F4-
P123；
(6) plant（5）F obtained by screening4-P123、F4- P456, florescence respectively selects 3 plants excellent of individual plant of economical character, makees miscellaneous
Hand over F4-P123/F4- P456, must reestablish diplomatic relations F1, with F1- P123456 is represented；
(7) F is planted1- P123456 seeds, florescence bagging selfing, individual plant sowing obtains F2For seed F2-P123456；
(8) F is planted2320 plants of-P123456, and Resistance Identification is carried out, 3 resistant parent institute disease resistance species are identified, filter out
Simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, 30 sets of individual plant that silique is more, seed is big
Bag selfing, period of maturation individual plant numbering sowing selects the seed of performance yellow grain characters in the seed of results, obtain the F of choosing3Generation
Seed F3-P123456；
(9) F is planted3250 plants of-P123456, carries out sclerotiniose, virosiss, white blister Resistance Identification, filters out high anti-3 kinds of diseases
Evil, many tall and big compact, effective branches of plant type, main inflorescence length, 30 bagging selfings of individual plant that silique is more, seed is big, period of maturation
Individual plant numbering sowing, selects the performance unseparated individual plant of yellow grain characters in the seed of results, carry out respectively Oleic acid, erucic acid,
Glucosinolate content is determined, and is filtered out oleic acid content more than 80%, content of erucic acid and is less than 20 μm of ol.g less than 0.5%, glucosinolate content-1List
Strain 5, obtains the F of choosing4For seed F4-P123456；
(10) strain plantation F4- P123456,180 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institute disease resistances
Species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed is big
20 bagging selfings of individual plant, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, seed difference
Carry out Oleic acid, erucic acid, glucosinolate content to determine, filter out oleic acid content more than 80%, content of erucic acid low less than 0.5%, glucosinolate content
In 20 μm of ol.g-1Individual plant 5, obtain the F of choosing5For seed F5-P123456；
(11) F selected in strain plantation5- P123456,100 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institutes
Disease resistance species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 20 bagging selfings of individual plant of grain, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, plants
Son carries out respectively Oleic acid, erucic acid, glucosinolate content and determines, and filters out oleic acid content more than 80%, content of erucic acid less than 0.5%, sulfur glycoside
Content is less than 20 μm of ol.g-1Individual plant 5, obtain the F of choosing6For seed F6-P123456；
(12) F selected in strain plantation6- P123456,80 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institutes
Disease resistance species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 10 bagging selfings of individual plant of grain, period of maturation individual plant numbering sowing, seed carries out respectively Oleic acid, erucic acid, glucosinolate content and determines,
Filter out oleic acid content more than 80%, content of erucic acid and be less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 2, that is, be bred as
High yield, yellow seed, high oleic acid, low erucic acid, low sulfatide, anti-sclerotinia rot of colza, Rape virus disease, the height of white rust of colza resist double low
Yellow seed rape kind " yellow oil resistant 1 ".
" yellow oil resistant 1 " yield is high, the kghm of average yield per mu oil mass 3069. 1-2, than compareing Zhejiang University 619
（2690.3kg·hm-2）Increase by 14. 08%, better resistance, high anti-sclerotinia rot of colza, virosiss and white blister, good quality, erucic acid
Content 0.42%, 18.90 μm of ol.g of glucosinolate content-1, oil content 48.12%, oleic acid content is up to 81%, per mu can reduce dispenser into
This 100 yuan, Semen Brassicae campestriss purchasing price increases 25-30%, and per mu can increase by 360 yuan or so of economic benefit, while reducing pesticide pair
The pollution of environment.
Embodiment 2：
(1) yellow-seeded brassica napus kind YR5602 is selected（P1）, rape high oleic acid kind FU012（P2）, low erucic acid, low sulfatide product
Plant Zhejiang University 619（P3）, it is double No. 9 in anti-sclerotinia rot of colza kind（P4）, oil resistant dish virosiss kind Lingot（P5）, oil resistant dish
White blister kind east spoke one（P6）For hybrid strain, make maternal with P1, make hybridization P1/P2, P1/P3, make maternal with P4, make miscellaneous
Hand over P4/P5, make hybridization P4/P6, obtain 4 hybridization F1, respectively with F1-P12、F1-P13、F1-P45、F1- P46 is represented；
(2) F is planted1-P12、F1-P13、F1-P45、F1- P46 is each 10 plants, and the florescence makees hybridization P12/P13, P45/P46, must reestablish diplomatic relations
F1For seed F1-P123、F1-P456；
(3) F is planted1-P123、F1- P456, florescence bagging selfing, period of maturation individual plant sowing obtains F2For seed F2-P123、F2-
P456；
(4) F is planted respectively2For seed F2-P123、F2- P456, each 200 plants of combination plantation, combines F2- P456 carries out sclerotium
Disease, virosiss, white blister Resistance Identification, filter out high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length,
10 bagging selfings of individual plant that silique is more, seed is big, individual plant sowing obtains F3For seed F3-P456；Combination F2- P123 baggings are certainly
Hand over, the seed of performance yellow grain characters is selected in the seed of results, obtain the F of choosing3For seed F3-P123；
(5) F is planted3-P123、F3- P456, each 200 plants of combination plantation, combines F3- P456 carries out sclerotiniose, virosiss, white rust
Sick Resistance Identification, filters out that high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed is big
10 bagging selfings of individual plant, individual plant sowing obtains F4For seed F4-P456；Combination F3- P123 bagging selfings, in the seed of results
The unseparated individual plant of performance yellow grain characters is selected, Oleic acid, erucic acid, glucosinolate content is carried out respectively and is determined, filtered out Oleic acid and contain
Amount more than 80%, content of erucic acid is less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 5, obtain the F of choosing4For seed F4-
P123；
(6) plant（5）F obtained by screening4-P123、F4- P456, florescence respectively selects 3 plants excellent of individual plant of economical character, makees miscellaneous
Hand over F4-P123/F4- P456, must reestablish diplomatic relations F1, with F1- P123456 is represented；
(7) F is planted1- P123456 seeds, florescence bagging selfing, individual plant sowing obtains F2For seed F2-P123456；
(8) F is planted2380 plants of-P123456, and Resistance Identification is carried out, 3 resistant parent institute disease resistance species are identified, filter out
Simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique is more, seed is big individual plant 30-40
Bagging selfing, period of maturation individual plant numbering sowing selects the seed of performance yellow grain characters in the seed of results, obtain the F of choosing3
For seed F3-P123456；
(9) F is planted3300 plants of-P123456, carries out sclerotiniose, virosiss, white blister Resistance Identification, filters out high anti-3 kinds of diseases
Evil, many tall and big compact, effective branches of plant type, main inflorescence length, 40 bagging selfings of individual plant that silique is more, seed is big, period of maturation
Individual plant numbering sowing, selects the performance unseparated individual plant of yellow grain characters in the seed of results, carry out respectively Oleic acid, erucic acid,
Glucosinolate content is determined, and is filtered out oleic acid content more than 80%, content of erucic acid and is less than 20 μm of ol.g less than 0.5%, glucosinolate content-1List
Strain 5, obtains the F of choosing4For seed F4-P123456；
(10) strain plantation F4- P123456,180 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institute disease resistances
Species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed is big
25 bagging selfings of individual plant, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, seed difference
Carry out Oleic acid, erucic acid, glucosinolate content to determine, filter out oleic acid content more than 80%, content of erucic acid low less than 0.5%, glucosinolate content
In 20 μm of ol.g-1Individual plant 5, obtain the F of choosing5For seed F5-P123456；
(11) F selected in strain plantation5- P123456,100 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institutes
Disease resistance species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 20 bagging selfings of individual plant of grain, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, plants
Son carries out respectively Oleic acid, erucic acid, glucosinolate content and determines, and filters out oleic acid content more than 80%, content of erucic acid less than 0.5%, sulfur glycoside
Content is less than 20 μm of ol.g-1Individual plant 5, obtain the F of choosing6For seed F6-P123456；
(12) F selected in strain plantation6- P123456,80 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institutes
Disease resistance species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 10 bagging selfings of individual plant of grain, period of maturation individual plant numbering sowing, seed carries out respectively Oleic acid, erucic acid, glucosinolate content and determines,
Filter out oleic acid content more than 80%, content of erucic acid and be less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 3, that is, be bred as
High yield, yellow seed, high oleic acid, low erucic acid, low sulfatide, anti-sclerotinia rot of colza, Rape virus disease, the height of white rust of colza resist double low
Yellow seed rape kind " yellow oil resistant 2 ".
" yellow oil resistant 2 " yield is high, the kghm of average yield per mu oil mass 3290. 8-2, than compareing Zhejiang University 619
（2690.3kg·hm-2）Increase by 22.3%, better resistance, high anti-sclerotinia rot of colza, virosiss and white blister, good quality, erucic acid contains
Amount 0.31%, 16.78 μm of ol.g of glucosinolate content-1, oil content 48.82%, oleic acid content is up to 82%, and per mu can reduce dispenser cost
100 yuan, Semen Brassicae campestriss purchasing price increases 25-30%, and per mu can increase by 420 yuan or so of economic benefit, while reducing pesticide to ring
The pollution in border.
Embodiment 3：
(1) yellow-seeded brassica napus kind YR5602 is selected（P1）, rape high oleic acid kind FU012（P2）, low erucic acid, low sulfatide product
Plant Zhejiang University 619（P3）, it is double No. 9 in anti-sclerotinia rot of colza kind（P4）, oil resistant dish virosiss kind Lingot（P5）, oil resistant dish
White blister kind Regent（P6）For hybrid strain, make maternal with P1, make hybridization P1/P2, P1/P3, make maternal with P4, make to hybridize
P4/P5, make hybridization P4/P6, obtain 4 hybridization F1, respectively with F1-P12、F1-P13、F1-P45、F1- P46 is represented；
(2) F is planted1-P12、F1-P13、F1-P45、F1- P46 is each 10 plants, and the florescence makees hybridization P12/P13, P45/P46, must reestablish diplomatic relations
F1For seed F1-P123、F1-P456；
(3) F is planted1-P123、F1- P456, florescence bagging selfing, period of maturation individual plant sowing obtains F2For seed F2-P123、F2-
P456；
(4) F is planted respectively2For seed F2-P123、F2- P456, each 180 plants of combination plantation, combines F2- P456 carries out sclerotium
Disease, virosiss, white blister Resistance Identification, filter out high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length,
10 bagging selfings of individual plant that silique is more, seed is big, individual plant sowing obtains F3For seed F3-P456；Combination F2- P123 baggings are certainly
Hand over, the seed of performance yellow grain characters is selected in the seed of results, obtain the F of choosing3For seed F3-P123；
(5) F is planted3-P123、F3- P456, each 180 plants of combination plantation, combines F3- P456 carries out sclerotiniose, virosiss, white rust
Sick Resistance Identification, filters out that high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed is big
10 bagging selfings of individual plant, individual plant sowing obtains F4For seed F4-P456；Combination F3- P123 bagging selfings, in the seed of results
The unseparated individual plant of performance yellow grain characters is selected, Oleic acid, erucic acid, glucosinolate content is carried out respectively and is determined, filtered out Oleic acid and contain
Amount more than 80%, content of erucic acid is less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 5, obtain the F of choosing4For seed F4-
P123；
(6) plant（5）F obtained by screening4-P123、F4- P456, florescence respectively selects 3 plants excellent of individual plant of economical character, makees miscellaneous
Hand over F4-P123/F4- P456, must reestablish diplomatic relations F1, with F1- P123456 is represented；
(7) F is planted1- P123456 seeds, florescence bagging selfing, individual plant sowing obtains F2For seed F2-P123456；
(8) F is planted2350 plants of-P123456, and Resistance Identification is carried out, 3 resistant parent institute disease resistance species are identified, filter out
Simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, 35 sets of individual plant that silique is more, seed is big
Bag selfing, period of maturation individual plant numbering sowing selects the seed of performance yellow grain characters in the seed of results, obtain the F of choosing3Generation
Seed F3-P123456；
(9) F is planted3280 plants of-P123456, carries out sclerotiniose, virosiss, white blister Resistance Identification, filters out high anti-3 kinds of diseases
Evil, many tall and big compact, effective branches of plant type, main inflorescence length, 35 bagging selfings of individual plant that silique is more, seed is big, period of maturation
Individual plant numbering sowing, selects the performance unseparated individual plant of yellow grain characters in the seed of results, carry out respectively Oleic acid, erucic acid,
Glucosinolate content is determined, and is filtered out oleic acid content more than 80%, content of erucic acid and is less than 20 μm of ol.g less than 0.5%, glucosinolate content-1List
Strain 5, obtains the F of choosing4For seed F4-P123456；
(10) strain plantation F4- P123456,180 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institute disease resistances
Species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed is big
22 bagging selfings of individual plant, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, seed difference
Carry out Oleic acid, erucic acid, glucosinolate content to determine, filter out oleic acid content more than 80%, content of erucic acid low less than 0.5%, glucosinolate content
In 20 μm of ol.g-1Individual plant 5, obtain the F of choosing5For seed F5-P123456；
(11) F selected in strain plantation5- P123456,100 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institutes
Disease resistance species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 20 bagging selfings of individual plant of grain, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, plants
Son carries out respectively Oleic acid, erucic acid, glucosinolate content and determines, and filters out oleic acid content more than 80%, content of erucic acid less than 0.5%, sulfur glycoside
Content is less than 20 μm of ol.g-1Individual plant 5, obtain the F of choosing6For seed F6-P123456；
(12) F selected in strain plantation6- P123456,80 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institutes
Disease resistance species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 10 bagging selfings of individual plant of grain, period of maturation individual plant numbering sowing, seed carries out respectively Oleic acid, erucic acid, glucosinolate content and determines,
Filter out oleic acid content more than 80%, content of erucic acid and be less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 2, that is, be bred as
High yield, yellow seed, high oleic acid, low erucic acid, low sulfatide, anti-sclerotinia rot of colza, Rape virus disease, the height of white rust of colza resist double low
Yellow seed rape kind " yellow oil resistant 3 ".
" yellow oil resistant 3 " yield is high, the kghm of average yield per mu oil mass 3184. 6-2, than compareing Zhejiang University 619
（2690.3kg·hm-2）Increase by 18. 37%, better resistance, high anti-sclerotinia rot of colza, virosiss and white blister, good quality, erucic acid
Content 0.34%, 18.74 μm of ol.g of glucosinolate content-1, oil content 48.72%, oleic acid content is up to 80%, and Semen Brassicae campestriss purchasing price increases
Plus 25-30%, per mu can increase by 390 yuan or so of economic benefit, while reducing pollution of the pesticide to environment.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (1)
1. a kind of selection of high anti-double low yellow seed rape kinds, it is characterised in that the selection include following methods with
Step：
（1）Juvenile stage：Select yellow-seeded brassica napus kind YR5602 is for P1, rape high oleic acid kind FU003 or FU012
P2, low erucic acid, low sulfatide kind Zhejiang University 619 are P3, and double No. 9 is P4 in anti-sclerotinia rot of colza kind, oil resistant dish virosiss kind
Lingot is P5, and anti-white rust of colza kind Regent or eastern spoke one are P6, make maternal with P1, make hybridization P1/P2, P1/P3,
Make maternal with P4, make hybridization P4/P5, make hybridization P4/P6, obtain 4 hybridization F1, respectively with F1-P12、F1-P13、F1-P45、F1-
P46 is represented；
（2）Plantation F1-P12、F1-P13、F1-P45、F1- P46 is each 10 plants, and the florescence makees hybridization P12/P13, P45/P46, and must reestablish diplomatic relations F1
For seed F1-P123、F1-P456；
（3）Plantation F1-P123、F1- P456, florescence bagging selfing, period of maturation individual plant sowing obtains F2For seed F2-P123、F2-
P456；
（4）F is planted respectively2For seed F2-P123、F2- P456, each combination plantation 150-200 strain, combines F2- P456 carries out bacterium
Core disease, virosiss, white blister Resistance Identification, filter out that high anti-3 kinds of diseases, tall and big compact, the effective branch of plant type be more, main inflorescence
Long, 10 bagging selfings of individual plant that silique is more, seed is big, individual plant sowing obtains F3For seed F3-P456；Combination F2- P123 baggings
Selfing, selects the seed of performance yellow grain characters in the seed of results, obtain the F of choosing3For seed F3-P123；
（5）Plantation F3-P123、F3- P456, each combination plantation 150-200 strain, combines F3- P456 carry out sclerotiniose, virosiss,
White blister Resistance Identification, filters out that high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 10 bagging selfings of individual plant of grain, individual plant sowing obtains F4For seed F4-P456；Combination F3- P123 bagging selfings, the kind of results
The unseparated individual plant of performance yellow grain characters is selected in son, Oleic acid, erucic acid, glucosinolate content is carried out respectively and is determined, screened fuel-displaced
Acid content more than 80%, content of erucic acid is less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 5, obtain the F of choosing4In generation, plants
Sub- F4-P123；
（6）Plantation（5）F obtained by screening4-P123、F4- P456, florescence respectively selects 3 plants excellent of individual plant of economical character, makees to hybridize
F4-P123/F4- P456, must reestablish diplomatic relations F1, with F1- P123456 is represented；
（7）Plantation F1- P123456 seeds, florescence bagging selfing, individual plant sowing obtains F2For seed F2-P123456；
（8）Plantation F2- P123456 320-380 strains, and Resistance Identification is carried out, identify 3 resistant parent institute disease resistance species, sieve
Select simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, individual plant 30- that silique is more, seed is big
40 bagging selfings, period of maturation individual plant numbering sowing selects the seed of performance yellow grain characters in the seed of results, obtain choosing
F3For seed F3-P123456；
（9）Plantation F3- P123456 250-300 strains, carry out sclerotiniose, virosiss, white blister Resistance Identification, filter out high anti-3 kinds
Tall and big compact, the effective branch of disease, plant type is more, main inflorescence length, 30-40 bagging selfing of individual plant that silique is more, seed is big, into
Ripe phase individual plant numbering sowing, selects the unseparated individual plant of performance yellow grain characters in the seed of results, Oleic acid, mustard are carried out respectively
Acid, glucosinolate content are determined, and are filtered out oleic acid content more than 80%, content of erucic acid and are less than 20 μm of ol.g less than 0.5%, glucosinolate content-1
Individual plant 5, obtain the F of choosing4For seed F4-P123456；
（10）Strain plants F4- P123456,180 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institute disease resistances
Species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed is big
20-25 bagging selfing of individual plant, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, seed
Oleic acid, erucic acid, glucosinolate content are carried out respectively to determine, and are filtered out oleic acid content more than 80%, content of erucic acid and are contained less than 0.5%, sulfur glycoside
Amount is less than 20 μm of ol.g-1Individual plant 5, obtain the F of choosing5For seed F5-P123456；
（11）The F selected in strain plantation5- P123456,100 plants of each strain carries out Resistance Identification, identifies 3 resistant parent institutes
Disease resistance species, filters out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
The big 20 bagging selfings of individual plant of grain, period of maturation individual plant numbering sowing selects the unseparated individual plant of performance yellow grain characters, plants
Son carries out respectively Oleic acid, erucic acid, glucosinolate content and determines, and filters out oleic acid content more than 80%, content of erucic acid less than 0.5%, sulfur glycoside
Content is less than 20 μm of ol.g-1Individual plant 5, obtain the F of choosing6For seed F6-P123456；
（12）The F selected in strain plantation6- P123456,80 plants of each strain carries out Resistance Identification, and 3 resistant parents of identification resist
Disease species, filter out that simultaneously high anti-3 kinds of diseases, many tall and big compact, effective branches of plant type, main inflorescence length, silique be more, seed
Big 10 bagging selfings of individual plant, period of maturation individual plant numbering sowing, seed carries out respectively Oleic acid, erucic acid, glucosinolate content and determines, sieve
Select oleic acid content more than 80%, content of erucic acid and be less than 20 μm of ol.g less than 0.5%, glucosinolate content-1Individual plant 2-3, that is, be bred as
High yield, yellow seed, high oleic acid, low erucic acid, low sulfatide, anti-sclerotinia rot of colza, Rape virus disease, the height of white rust of colza resist double low
Yellow seed rape kind.
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| CN111789023A (en) * | 2020-07-27 | 2020-10-20 | 西南大学 | Breeding method of yellow-seed high-oleic-acid cabbage type rape |
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