KR101432278B1 - New plant variety of rape - Google Patents

Abstract

The present invention provides a new variety of rapeseed which is obtained by interspecific hybridization of red cabbage and Chinese cabbage with high fatty acid content and increased seed size and has the following botanical characteristics.

Description

New rapeseed varieties {New plant variety of rape}

The present invention relates to novel rapeseed varieties, and more particularly to novel rapeseed varieties by hybridization of red cabbage and Chinese cabbage.

Of chromosome structure and evolution of crops belonging to baechusok there is the description "Wu triangle (triangle of U) 'model of Woo Jang-choon Dr. According rapeseed (B. napus) is Chinese cabbage (B. rapa) and cabbage (B. oleracea) It is known to be caused by natural hybridization. The origin of the rapeseed (Brassica napus) was unclear, but after natural crosses between the two diploid species, B. oleracea (CC genome) and B. rapa (AA genome), which were growing close together, . After pairing two species with different genomes, doubling the number of chromosomes in the hybrid produced amphidiploids, a special case of polyploidy, and this doubling of chromosomal settings resulted in stable meiosis, To reproduce new multiparticulates. Hybridizations that do not double their chromosomes are likely to have been withdrawn by producing offspring without fertility. Cytogenetic studies on B. napus showed that both the AA genotype and the CC genome had both double genotypes extracted from the monogenomic species B. oleracea (CC genome) and B. rapa (AA genome) (Nagaharu U, Japan, J. Bot., 7: 389-452, 1935). Rapeseed has 19 pairs of chromosomes (2n = 38) and the entire base pair is estimated to be about 1,100 Mbp (Arumuganathan and Earle, Mol. Biol. Rep., 9: 208-218, 1991).

As the embryo develops in the seed, the metabolism in the cell becomes active, and the proplastid is differentiated into chloroplasts, and the synthesis of starch with photosynthesis is achieved. The starch produced from the chloroplast is used for the synthesis of triacylglyceride (TAG) and fatty acid in the cytoplasm. A large part of the lipids thus formed constitute an oil body, which is used for the purpose of nutrient accumulation to be used in future germination. In oilseed rape, the oil content of matured seeds reaches about 40%. As a result, these oils have attracted much attention as raw materials for biodiesel as well as for food.

Rapeseed seeds contain various fatty acids, most of which are present as unsaturated fatty acids. Fatty acids in oil crops including rapeseed are palmitic acid (C16: 0), stearic acid (C18: 0) and oleic acid (C18: 0) depending on the length of the hydrocarbon and the number of double bonds. 1), linoleic acid (C18: 2), linolenic acid (C18: 3), and erucic acid (C22: 1) Because of the different physicochemical properties of these various fatty acids, the range of application varies depending on the fatty acid composition of the oil extracted from the plant.

The oilseed rape cultivated in the past, including wild species, is known to induce lipidosis in humans and livestock, although the content of erucic acid is high. Recently, edible oilseed rape cultivated with very low erucic acid content Mostly. Rape varieties with high oleic acid content are cultivated worldwide for biodiesel fuel, and varieties of lecithin rich in erucic acid are used as raw materials for cosmetics raw materials.

It is an object of the present invention to provide a new variety of rapeseed which has increased erucic acid content. However, these problems are exemplary and do not limit the scope of the present invention.

According to one aspect of the present invention, there is provided a new variety of rapeseed obtained by interspecific crossing between red cabbage ( Brassica oleracea ) and Chinese cabbage ( Brassica rapa ) and having the following botanical characteristics.

(A) the vein and petiole are pink; (b) the sheep is 5.0 g or more; (c) the erucic acid content is 60% or more; And (d) a palmitoleic acid content of 0.1 to 1%.

According to another aspect of the present invention, there is provided a cosmetic composition comprising the above-described new rape variety extract as an active ingredient.

Since the new rape varieties have a higher erucic acid content than conventional rape varieties, erucic acid is a fatty acid used as a raw material for cosmetics and the like, so that a new variety of rapeseed according to one embodiment of the present invention can be used for the production of a cosmetic composition.

According to another aspect of the present invention there is provided a process for the production of rapeseed oil, comprising selecting rapeseed obtained by interspecific crossing of red cabbage ( Brassica oleracea ) and Chinese cabbage ( Brassica rapa ) and having one or more of the following botanical characteristics: A breeding method of a new variety is provided:

(a) the veins and petiole are pink;

(b) 5.0 g or more of ginseng;

(c) an erucic acid content of 50% or more; And

(d) palmitoleic acid content of 0.1 to 1%.

According to another aspect of the present invention, there is provided a rapeseed plant obtained by cross-breeding a new varieties of rapeseed according to an embodiment of the present invention as a pollen, a specimen or a duplicate.

The new varieties of rape are as described above.

According to one embodiment of the present invention as described above, a new variety of oilseed rape having increased seed size and fatty acid content can be realized. Of course, the scope of the present invention is not limited by these effects.

1 is a diagram showing a cross-sectional diagram of a new variety of rapeseed, according to an embodiment of the present invention.
Fig. 2 is a chart showing seed loss of the second plant (a), which has high autofluoscence, and the third plant (b), which has high self-moisture content.
FIG. 3 is a view showing a seedling growing state of a new variety of rape seedlings according to an embodiment of the present invention.
FIG. 4 is a view (B) showing a breeding appearance of a new variety of rape variety according to an embodiment of the present invention and a color of a vein and a petiole of a dainty plant.
FIG. 5 is a diagram comparing the colors of vein and petiole of Dala rapeseed and rapeseed rape.
Fig. 6 is a diagram comparing leaves of rape seedlings and hail rapeseed.
FIG. 7 is an analysis of the karyotype of Korean red cabbage, Chinese cabbage and Chinese cabbage according to an embodiment of the present invention.
8 is a diagram showing the results of molecular biology analysis using SNP (a) and RAPD (b) of rape seedlings and red cabbage and Chinese cabbage according to an embodiment of the present invention;
Red arrow: Unusual bands of savory cabbage;
Green arrow: Singular band of Chinese cabbage;
Red and green dots: specific bands of amplified specimens and duplicates in rape; And
Blue Box: Distinguished Rapeseed Singles Band.
FIG. 9 is a RAPD analysis of various kinds of rapeseed varieties using a primer amplifying a specific rape band.

General method

Hereinafter, the general method used in the present invention will be described in detail.

1. Intergeneric hybridization and immature culture

Using B. oleracea as a template, artificial watering of the cabbage ( B.rapa ) as a duplicate leads to the in vitro culture of the fertilized pod under immature conditions. After 14 to 21 days from the date of crossing, the modified pods were taken and the embryos were removed from them and placed on MS medium. In-vitro culture conditions are controlled at 25 ± 1 ° C, luminous intensity of 40 μmol · m ^-2 · s ^-1 , and light period of 16 hours. Plants from immature embryos were cultured in a larger culture bottle. Afterwards, they are formalized and cultivated through purification process and nursery process.

2. Molecular genetic analysis using RAPD (Random Amplified Polymorphic DNA)

As a kind of PCR reaction, it is distinguished from conventional PCR in that the amplified DNA fragment is random. RAPD is performed in a PCR reaction with several arbitrary short primers (8-12 nucleotides) and as a template of large genomic DNA, and analyzing the resulting pattern of reaction results in obtaining a semi-unique profile from the RAPD reaction. The DNA sequence information is unnecessary because it binds to any of the DNA sequences of the target gene, although it does not know exactly where the primer is. Recently, RAPD has been used to identify and trace the phylogeny of various plant and animal species.

In the present invention, RAPD analysis on genomic DNA was performed by the following method:

1) PCR reaction liquid composition

The PCR reaction composition was prepared by mixing 50 ng of template DNA, 10 μl PCR buffer (containing 1.5 mM MgCl 2), 200 μM of dNTPs, 2 units of taq DNA polymerase and 20 μM of RAPD primer and adjusting the total volume to 20 μl with distilled water (DW) Liquid.

2) Performing the PCR reaction

The PCR reaction solution was put into a PCR tube and denatured at 95 ° C for 3 minutes. The PCR reaction solution was denatured at 95 ° C for 1 minute, 35 ° C for 1 minute, and 72 ° C for 1 minute 30 seconds. 30 seconds at 40 ° C for 20 seconds, and 72 ° C for 1 minute. The reaction is carried out for 30 minutes at 72 ° C for 7 minutes.

3) Electrophoresis

20 μl of the PCR reaction was loaded on a 2.0% (w / v) agarose gel, and electrophoresis was carried out at a voltage of 200 V under 0.5 × TBE buffer for 1 hour and 30 minutes. At this time, 6 ㎕ of 1Kb ladder is used as a marker. After the electrophoresis, the gel is stained with EtBr solution, and the gel is placed on the fluorescence, and an image is obtained using an imager.

Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings. It should be understood, however, that the present invention is not limited to the embodiments shown in the drawings, but may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. It is provided to fully inform the category of invention to a knowledgeable person. Also, for convenience of explanation, the components may be exaggerated or reduced in size.

1 is a diagram showing a cross-sectional diagram of a new variety of rapeseed, according to an embodiment of the present invention. The present inventors removed the pollen from the buds of B. oleracea and used it as a template to breed rapeseeds having a large seed size, such as red cabbage, and performed interspecific cross-breeding of B.rapa . As a result, it was possible to obtain three plants. From the left side of F2 in Fig. 1, the first plant was dead during winter, the second plant was high in self-incompatibility, It was possible to obtain a large amount of seed later because it had a high self - moisture rate.

Fig. 2 is a chart showing seed loss of the second plant (a), which has high autofluoscence, and the third plant (b), which has high self-moisture content. As shown in Fig. 1, seedlings of the obtained plants were observed as a result of interspecific interbreeding between red cabbage and Chinese cabbage.

FIG. 3 is a view showing a seedling growing state of a new variety of rape seedlings according to an embodiment of the present invention. As a result of observing the breeding characteristics of the interspecific hybrid of the present invention, it was observed that the back side of the leaf was pink in the seedling stage.

FIG. 4 is a view (B) showing a breeding appearance of a new variety of rape variety according to an embodiment of the present invention and a color of a vein and a petiole of a dainty plant. As a result of observing the breeding characteristics of the interspecific crosses according to one embodiment of the present invention, it was confirmed that the veins and petioles are pinkish from the beginning of growth to the growth of the flowering period.

Figs. 5 and 6 are diagrams comparing the color vein color and leaf shape of the leaf vein, the petiole, and the leaves of the rapeseed rape and rapeseed rape. The breed characteristics of the interspecific crosses according to one embodiment of the present invention were compared with that of the halae oilseed rape. There was no difference in the leaf width, flower color, and seed color, but the leaf did not have a difference in the ripening phase, but the vein and petiole of the daphnia rapeseed were pinkish, sawtooth shape of the leaf edge was more dense, rapeseed was more flat, .

FIG. 7 is an analysis of the karyotype of Korean red cabbage, Chinese cabbage and Chinese cabbage according to an embodiment of the present invention. In accordance with one embodiment of the present invention, rape seeds were removed from flower buds of red cabbage ( B. oleracea ) and used as a sample , and cabbage ( B.rapa ) was used as a duplicate. The chromosomal numbers of Daea were confirmed to be diploid of cabbage and Chinese cabbage, and 2n = 38 of diploids of red cabbage (2n = 18) and Chinese cabbage (2n = 20)

8 is a diagram showing the results of molecular biology analysis using SNP (a) and RAPD (b) of rape seedlings and red cabbage and Chinese cabbage according to an embodiment of the present invention. As a result of analyzing the sample cabbage, the Chinese cabbage and the shallow rape according to the High Resolution Melting (HRM) method, it was confirmed that the rape seed according to the embodiment of the present invention is a heterozygote of the above-described specimen and copepiece. Also, genomic DNA of rapeseed rape was analyzed using RAPD method. As a result, it was confirmed that Daea amplifies the specific band of cabbage and cabbage. In addition, the results of comparative analysis of Daehae, Youngsan, Tammi, Halla, Kangyoo, Enamel, Resandra, and Jeju Halla varieties using the primer B01 amplified with the rape specific band in Fig. It was confirmed that the rape-specific band was amplified.

FIG. 9 is a RAPD analysis of various kinds of rapeseed varieties using a primer amplifying a specific rape band.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. It should be understood, however, that the invention is not limited to the disclosed embodiments and examples, but may be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, It is provided to fully inform the owner of the scope of the invention.

Example 1: Hybridization of cabbage and Chinese cabbage and production of new rapeseed plants

The present inventors conducted interspecific interbreeding of B. raea cabbage ( B. oleracea ) and Chinese cabbage ( B.rapa ) in order to grow rapeseeds having a large seed size such as red cabbage. After removing the pollen from the bud of red cabbage, it was used as a sample.

The pods formed after 14-21 days from the date of artificial mating were obtained before degeneration, and the immature pod culture was performed in the medium, from which three plants could be obtained (see Fig. 1). 2), it was difficult to obtain seeds because of high self-incompatibility (see Fig. 2 (a)), and the third plant has a high self-water content, , And named it "Daina" (see FIG. 2 (B)).

Example 2: Cultivar characteristics

The interspecific crossbreeding according to one embodiment of the present invention confirmed the uniformity among the plants as a result of the subsequent plant cultivation. The new varieties Daya rapeseed is characterized by a pinkish appearance on the back of the leaf in the seedling stage (Fig. 3), and veins and petioles ranging from the early stage of growth to the growth of the flowering period (Fig. There was no difference in the leaf width, flower color, and seed color of leaves, but there was no difference in the ripening phase of the leaves. The leaf veins and petiole of the seedlings were pinkish and sawtooth shape And the upper part of the leaf was close to a circle (Figs. 5 and 6).

In addition, according to the embodiment of the present invention, since the plant is thick and strong, the posture of the plant is straight and the sagging degree is small. Since the growth period was longer than 80 days until the flowering stage, the seeds produced were vigorous, and the seeds produced were somewhat larger than the general rapeseed.

Comparison of Botanical Characteristics of Daya Aegean and Halla rapeseed Shape Appearance A lot of oilseed rape Halla oilseed rape One 2 3 4 5 6 7 8 9 Expression form Expression form One Width of cotyledon
narrowness
middle
broadness
5 5 2 Leaf border
Sawtooth
none
slightly
has exist
medium
liver
many
2 3 3 Lobe of leaves
none,
circle
none,
Jangran Type Has a little
medium
liver
A lot of
6 6 4 Leaf color Perch rust Enrichment 8 7 5 bloom prematurity Maturity Manju 5 6 Flower Sigh sulfur Redness 5 5 7 Maturity prematurity Maturity Manju 7 4 8 Color of seed coat Brown Red black yellow 4 4 9 Chunpae Castle Very weak weakness middle Strong 2 5

Example 3:

In order to investigate the seed size of rice varieties, we compared the grain size of Korean rice varieties with those of Chinese cabbage (3.0g), cabbage (4.95g) and common rapeseed varieties (5.1g, 4.9g, 4.1g) (Table 2).

Kinds Ginseng (g) source Rapeseed Ferroelectricity 5.14 National seed source Cold weather 2.90 Rural Development Administration Amorous 4.00 Rural Development Administration Halla 2.90 Rural Development Administration Conversation 4.10 National seed source Envy 4.20 National seed source Dear 5.45 F & P Co., Ltd. lampshade Cheonggap 2.17 Gyeonggi Agricultural Research and Extension Services smile 2.20 F & P cabbage Common Chinese cabbage 3.00 - Chinese cabbage 3.65 Gyeonggi Agricultural Research and Extension Services cabbage Red cabbage 4.95 Gyeonggi Agricultural Research and Extension Services

Example 4: Fatty acid content analysis

The fatty acid content of 'Daya', a new variety of rapeseed according to an embodiment of the present invention, was analyzed. For comparison, fatty acids content of rapeseed, mustard, cabbage and cabbage cultivars described in Table 2 were analyzed.

Sodium sulfate was added to the seeds of each plant at a ratio of 8 to 10 times that of the seeds. After finely crushing the seeds in a mortar, the fat was extracted using the Soxhlet extraction method or hexane.

Pretreatment of fatty acids was performed by the same pretreatment method of 'Fatty acid second method and fat analysis method' and gas chromatography analysis was performed. The fatty acid analysis was performed using gas chromatography (Agilent 7890 GC, Agilent Technologies), HP-INNOWAX (30 mx 0.320 mm x 0.25 μm, Agilent Technologies) and a hydrogen flame ionization detector. The inlet temperature was 225 ° C and the detector temperature was 285 ° C. The oven temperature was maintained at 100 ° C for 4 minutes, then increased to 240 ° C at 3 ° C per minute for 15 minutes. The detector temperature was 285 ° C and nitrogen was used as carrier gas.

As a result, it was confirmed that the new varieties of oilseed rape according to one embodiment of the present invention contained 11.1% oleic acid and 63.6% erucic acid, and 0.32% palmitoleic acid, which is small in amount but not found in other plants . Palmitoleic acid is known to have an effect of preventing diabetes as an unsaturated fatty acid, and since erucic acid is a fatty acid used as a raw material for cosmetics and the like, it can be used for manufacturing cosmetics using a new variety of oilseed rape according to an embodiment of the present invention 3).

Fatty acid content analysis result Fatty acid content (%) Cold weather
Youngsan
Halla
S16
S19
S20
S21
smile
(lampshade)
Dear
C16: 0 palmitic acid
palmitic acid 4.1
4.0
6.4
7.7
4.6
6.2
6.4
2.3
6.32
C16: 1 palmitoleic acid
palmitoleic acid, -
-
-
-
-
-
-
-
0.32
C18: 0 stearic acid
(stearic acid) 2.2
1.9
1.9
1.6
1.8
2.01
1.57
0.9
1.63
C18: 1 oleic acid
oleic acid, 58.8
63.4
61.3
59.1
63.1
55.2
55.3
8.0
11.1
C18: 2 linoleic acid
linoleic acid, 17.4
19.1
23.0
23.2
20.2
20.6
19.2
15.8
4.4
C18: 3 linolenic acid
(linolenic acid) 8.1
7.3
-
-
6.7
-
1.5
9.4
5.4
C20: 0 arachidic acid
arachidic acid 0.8
0.7
5.3
7.7
0.6
8.36
11.9
0.8
1.53
C20: 1 Cis-11-Eicosenoic 3.8
1.3
-
-
1.1
-
-
5.2
1.06
C22: 0 behenic acid
(behenic acid) 0.4
-
-
-
0.3
-
-
1.2
1.47
C22: 1 Erusan
(eruic acid) 3.0
0.6
-
-
-
-
-
49.2
63.6
C24: 0 Lignoceric acid
(lignoceric acid) -
-
-
-
-
-
-
0.5
-
C24: 1 Nerubic acid
(nervonic acid) -
-
-
-
-
-
-
2.2
-

Example 5: Chromosome analysis

The order of chromosome analysis to confirm the multiplication of the number of chromosomes was as follows. The root tip was sampled at 5 mm and placed in a sample bottle filled with 3 ml of cold distilled water. The sample was then placed in an icebox filled with crushed ice and transported indoors. The apex was cut about 2 mm to obtain an Eppendorf tube (eppendorf tube). The prepared sample was placed in 0.8% bromonaphthalene and allowed to react at room temperature for 40 minutes. About 0.8% of bromonaphthalene was used in 1 ml of the solution and about 20-30 of the near 2 mm cuts were used. When the reaction was completed, the 0.8% bromonaphthalene solution was removed and immersed in sterilized water three times. Sterile water was removed and fixed with ethanol-glacial acetic acid at room temperature for one day. The next day, the cells were washed three times with sterilized water, and then enzyme solution (2% cellulase / 1% pectinase / 0.5% pectolyase) was added and dissociated at 37 ° C for 50 minutes. Suck the apical tip using a Pasteur pipette and drop only two on a clean slide. The filter paper was used to remove water to the extent that there was only one layer of film at the near end, and then the acetocarmine dyeing reagent was dropped. The muscles were stretched evenly with tweezers so that they spread evenly with the liquid. The prepared slides were photographed by counting chromosomes at a magnification of 400 using a phase difference microscope (Carl Zeiss), and injecting the milky lotion at a magnification of 1,000.

As a result, the number of chromosomes of interspecific hybrids were 2n = 38 (2n = 18) and 2n = 38 (2n = 20).

Example 6: SNP and RAPD analysis

6-1: SNP analysis

The High Resolution Melting (HRM) method was used to develop molecular markers associated with breedable loci and to analyze the genotypes of individual plants using LC480 (Roche). PCR was carried out with 5 쨉 l of template DNA, 10 pmoles of each primer (Table 4), 10 쨉 l of SYBR mixture, and 2 쨉 l of MgCl2 in a total volume. The PCR consisted of 3 minutes of reaction at 94 ° C, 45 cycles of 15 seconds at 94 ° C, 25 seconds at 53-58 ° C and 45 seconds at 72 ° C, and a reaction at 72 ° C for 5 minutes. The fluorescence signal was measured at 95 ° C for 1 minute and at 40 ° C for 1 minute, then increased from 65 ° C to 95 ° C by 0.02 ° C per second, and 25 ° C for every 1 ° C rise. Melting curve analysis was performed using the manufacturer's operating software .

Primers used for the analysis Name of the primer
SEQ ID NO:
Nucleic acid sequence
The amplified product (bp)
FPBPN0011-F
One
5'-GCATGGTGTGGATGAAGTTG-3 '
99bp
FPBPN0011-R
2
5'-TTTACCTAAGCATCAGAGGATGG-3 '

Primer for amplification of a site containing 99bp of base of FPBPN0011 which is a molecular marker capable of discriminating red cabbage, Chinese cabbage and rapeseed, and interspersed between double strands of the gene for amplification of the molecular marker FPBPN0011 to visualize gene amplification amount The labeling material (SYBR) was used. First, the FPBPN0011 molecular marker was PCR amplified, and then the temperature of the PCR amplification product was applied to release the double strand of FPBPN0011 into a single strand, thereby reducing the amount of labeling substance present between the double strands of FPBPN0011. And the amount of labeling substance present between the double strands of FPBPN0011 of the PCR amplification product as the temperature rises is used to calculate the 'amount of the labeled substance against FPBPN0011' of red cabbage, Chinese cabbage, and rapeseed The melting curve was determined. As a result, as shown in Fig. 8 (A), analysis of the sample cabbage, the cabbage, and the cabbage was found to be heterozygosity of cabbage and cabbage (Fig. 8 (a)).

6-2: RAPD analysis

RAPD was performed on the genomic DNA of red cabbage, Chinese cabbage and rape rape for the molecular genetic analysis of the new rapeseed varieties of the present invention. In this case, a kit sold by Operon was used as a primer, and the primer information used was as shown in Table 5.

Molecular biology analysis using RAPD revealed that it was a hybrid that amplified the specific band of cabbage and cabbage (Fig. 8 (b)). Using the B01 primer, we could identify a specific band of 980bp in the amplified bands. Using the B01 primer, it was confirmed that Daea japae only amplified the lecithin specific bands (Fig. 9). The results of the comparative analysis of Naehan, Youngsan, Ammi, Halla, Kangyu, Enamel, Risandra and Jeju Halla varieties. The B01 primer means OPB01 primer shown in Table 1 below, and A03 to B12 shown in FIG. 8 and FIG. 9 means OPA03 to OPB-12 primer.

Primers used for the analysis Name of the primer SEQ ID NO: Nucleic acid sequence Name of the primer SEQ ID NO: Nucleic acid sequence OPA-01 3 5'-CAGGCCCTTC-3 ' OPB-01 23 5'-GTTTCGCTCC-3 ' OPA-02 4 5'-TGCCGAGCTG-3 ' OPB-02 24 5'-TGATCCCTGG-3 ' OPA-03 5 5'-AGTCAGCCAC-3 ' OPB-03 25 5'-CATCCCCCTG-3 ' OPA-04 6 5'-AATCGGGCTG-3 ' OPB-04 26 5'-GGACTGGAGT-3 ' OPA-05 7 5'-AGGGGTCTTG-3 ' OPB-05 27 5'-TGCGCCCTTC-3 ' OPA-06 8 5'-GGTCCCTGAC-3 ' OPB-06 28 5'-TGCTCTGCCC-3 ' OPA-07 9 5'-GAAACGGGTG-3 ' OPB-07 29 5'-GGTGACGCAG-3 ' OPA-08 10 5'-GTGACGTAGG-3 ' OPB-08 30 5'-GTCCACACGG-3 ' OPA-09 11 5'-GGGTAACGCC-3 ' OPB-09 31 5'-TGGGGGACTC-3 ' OPA-10 12 5'-GTGATCGCAG-3 ' OPB-10 32 5'-CTGCTGGGAC-3 ' OPA-11 13 5'-CAATCGCCGT-3 ' OPB-11 33 5'-GTAGACCCGT-3 ' OPA-12 14 5'-TCGGCGATAG-3 ' OPB-12 34 5'-CCTTGACGCA-3 ' OPA-13 15 5'-CAGCACCCAC-3 ' OPB-13 35 5'-TTCCCCCGCT-3 ' OPA-14 16 5'-TCTGTGCTGG-3 ' OPB-14 36 5'-TCCGCTCTGG-3 ' OPA-15 17 5'-TTCCGAACCC-3 ' OPB-15 37 5'-GGAGGGTGTT-3 ' OPA-16 18 5'-AGCCAGCGAA-3 ' OPB-16 38 5'-TTTGCCCGGA-3 ' OPA-17 19 5'-GACCGCTTGT-3 ' OPB-17 39 5'-AGGGAACGAG-3 ' OPA-18 20 5'-AGGTGACCGT-3 ' OPB-18 40 5'-CCACAGCAGT-3 ' OPA-19 21 5'-CAAACGTCGG-3 ' OPB-19 41 5'-ACCCCCGAAG-3 ' OPA-20 22 5'-GTTGCGATCC-3 ' OPB-20 42 5'-GGACCCTTAC-3 '

The preparation examples for the composition of the present invention are illustrated below.

≪ Preparation Example 1 > Preparation of cosmetics

Skin whitening cosmetics containing the new variety rape seed extract according to one embodiment of the present invention as an active ingredient were prepared.

<1-1> Flexible lotion

The flexible lotion containing the new varieties rapeseed extract was prepared according to the composition shown in Table 6 below.

Raw material Content (% by weight) A variety of new varieties Rapeseed extract 10.00 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<2-2> Nourishing cream

The nutritional cream containing the new varieties rapeseed extract was prepared according to the composition shown in Table 7 below.

Raw material Content (% by weight) A variety of new varieties Rapeseed extract 10.0 1,3-butylene glycol 7. glycerin 1.0 D-Panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlyric / capric triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Preservative Suitable amount Flavoring agent Suitable amount Purified water Balance

While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the invention. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

<110> FNP. Inc <120> New plant variety of rape <130> PD12-0419 <160> 42 <170> Kopatentin 2.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> FPBPN0011-F <400> 1 gcatggtgtg gatgaagttg 20 <210> 2 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> FPBPN0011-R <400> 2 tttacctaag catcagagga tgg 23 <210> 3 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-01 <400> 3 caggcccttc 10 <210> 4 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-02 <400> 4 tgccgagctg 10 <210> 5 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-03 <400> 5 agtcagccac 10 <210> 6 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-04 <400> 6 aatcgggctg 10 <210> 7 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-05 <400> 7 aggggtcttg 10 <210> 8 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-06 <400> 8 ggtccctgac 10 <210> 9 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-07 <400> 9 gaaacgggtg 10 <210> 10 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-08 <400> 10 gtgacgtagg 10 <210> 11 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-09 <400> 11 gggtaacgcc 10 <210> 12 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-10 <400> 12 gtgatcgcag 10 <210> 13 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-11 <400> 13 caatcgccgt 10 <210> 14 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-12 <400> 14 tcggcgatag 10 <210> 15 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-13 <400> 15 cagcacccac 10 <210> 16 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-14 <400> 16 tctgtgctgg 10 <210> 17 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-15 <400> 17 ttccgaaccc 10 <210> 18 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-16 <400> 18 agccagcgaa 10 <210> 19 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-17 <400> 19 gaccgcttgt 10 <210> 20 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-18 <400> 20 aggtgaccgt 10 <210> 21 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-19 <400> 21 caaacgtcgg 10 <210> 22 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPA-20 <400> 22 gttgcgatcc 10 <210> 23 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-01 <400> 23 gtttcgctcc 10 <210> 24 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-02 <400> 24 tgatccctgg 10 <210> 25 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-03 <400> 25 catccccctg 10 <210> 26 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-04 <400> 26 ggactggagt 10 <210> 27 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-05 <400> 27 tgcgcccttc 10 <210> 28 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-06 <400> 28 tgctctgccc 10 <210> 29 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-07 <400> 29 ggtgacgcag 10 <210> 30 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-08 <400> 30 gtccacacgg 10 <210> 31 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-09 <400> 31 tgggggactc 10 <210> 32 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-10 <400> 32 ctgctgggac 10 <210> 33 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-11 <400> 33 gtagacccgt 10 <210> 34 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-12 <400> 34 ccttgacgca 10 <210> 35 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-13 <400> 35 ttcccccgct 10 <210> 36 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-14 <400> 36 tccgctctgg 10 <210> 37 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-15 <400> 37 ggagggtgtt 10 <210> 38 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-16 <400> 38 tttgcccgga 10 <210> 39 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-17 <400> 39 agggaacgag 10 <210> 40 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-18 <400> 40 ccacagcagt 10 <210> 41 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-19 <400> 41 acccccgaag 10 <210> 42 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> OPB-20 <400> 42 ggacccttac 10

Claims (4)

A new variety of rapeseed obtained by interspecific crossing between red cabbage ( Brassica oleracea ) and Chinese cabbage ( Brassica rapa ) and having the following botanical characteristics:
(a) the veins and petiole are pink;
(b) 5.0 g or more of ginseng;
(c) an erucic acid content of 60% or more; And
(d) palmitoleic acid content of 0.1 to 1%. A cosmetic composition comprising the novel oilseed rape extract of claim 1 as an active ingredient. delete A rapeseed plant which hybridized with a new varieties of rapeseed, 'Daea', as a pollen, a sample or a duplicate.

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