CN101869049A - Preparation method of cabbage type rape high erucic acid material - Google Patents
Preparation method of cabbage type rape high erucic acid material Download PDFInfo
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- CN101869049A CN101869049A CN 201010167890 CN201010167890A CN101869049A CN 101869049 A CN101869049 A CN 101869049A CN 201010167890 CN201010167890 CN 201010167890 CN 201010167890 A CN201010167890 A CN 201010167890A CN 101869049 A CN101869049 A CN 101869049A
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Abstract
The invention relates to a preparation method of a cabbage type rape high erucic acid material. FAD2 (delta 12 fatty acid desaturase) and FAD3 mutant genes are imported into a common high erucic acid material (the erucic acid content is about 50 percent) to make the synthesis of linoleic acid and linolenic acid in rapeseeds basically hindered to make the high erucic acid material synthetized into more erucic acid; and on the other hand, since the proportion of the linoleic acid to the linolenic acid in free fatty acid is reduced and the proportion of the erucic acid rises, the site 1 and the site 3 of a glycerol skeleton can be combined with sufficient erucic acid, thereby, the erucic acid content in the rapeseeds exceeds 60 percent. The invention can provide quality raw materials for industrial erucic acid manufacturing enterprises, reduces production cost and improves enterprise economic benefits and market competitiveness.
Description
Technical field
The present invention relates to Crop Genetic Breeding and agriculture Applied Biotechnology field, particularly relate to a kind of method for creating of cabbage type rape high erucic acid material.
Background technology
High erucic acid rapeseed oil can be used as the raw material of industrial lubricants, cosmetics, surfactant, plastics etc., has the wide industrial purposes.The manufacturer of domestic existing some special processing high erucic acid rapeseed oils, wherein, the high erucic acid oil that Sichuan Sipo Chemical Industry Co., Ltd. produces accounts for 1/8 of international market, accounts for 60% of market, Asia.According to the requirement of processing enterprise, content of erucic acid is high more good more in the rapeseed oil, and high erucic acid rapeseed oil can significantly cut down finished cost, and improves the quality of products, and then improves the performance of enterprises.
Seed selection high erucic acid rape kind is one of main target of domestic and international rapeseed breeding man always.The fatty acid building-up process of rape is as follows:
(FAE)
16: 1 → → 18: 0 → → 18: 1 (oleic acid) → → 20: 1 → → 22: 1 (erucic acid)
↓(FAD2)
18: 2 (linoleic acid)
↓(FAD3)
18: 3 (linolenic acid)
In the common rapeseed oil, content of erucic acid about 50%, oleic acid about 16%, linoleic acid about 13%, linolenic acid about 9%.
In recent years, for rapeseed oil alimentary health-care function and the requirement of prolongation shelf time, extensively carried out high oleic acid breeding both at home and abroad, by FAE, FAD2, FAD3 mutator gene (afunction) are aggregated to together, can create that oleic acid content reaches more than 85%, linoleic acid about 9%, linolenic acid are less than 3%, erucic acid is less than 1% new material.
By traditional breeding way, content of erucic acid can reach more than 55%, but will stablize very difficult above 60%.
Summary of the invention
The object of the present invention is to provide a kind of method for creating of cabbage type rape high erucic acid material, this method will have high-oleic acid material now and combine with the high erucic acid material, create stable 60% non-transgenic high erucic acid material and the kind of being higher than of content of erucic acid.
Core of the present invention and technical thought be, imports FAD2, FAD3 mutator gene in common high erucic acid material (about content of erucic acid 50%), makes in the rape seed linoleic acid and linolenic syntheticly be obstructed substantially, makes its synthetic more erucic acid; On the other hand, owing to linoleic acid plus linolenic acid ratio in the free fatty acid descends, the erucic acid ratio raises, and makes 1,3 potential energies of glycerol backbone enough in sufficient erucic acid, and content of erucic acid surpasses 60% in the rapeseed oil thereby make.
The method for creating of a kind of cabbage type rape high erucic acid of the present invention material is realized by following steps:
(1) it is maternal selecting the existing rape material of content of erucic acid more than 50%, is male parent with the rape mutant material of oleic acid content more than 80%, and hybridization obtains F1 for seed;
(2) plantation F1 is for plant, and F2 is for seed for results, and F2 is carried out half method gas chromatograph for determination for seed, selects the monoseeding of high erucic acid, low linoleic acid and low linolenic;
(3) florescence gets 3mm left and right sides flower pesticide and makes microspores culture, with microspore put into contain the 50mg/L colchicin the embryoid induction medium, in 32 ℃ incubator, handle 48h, finish chromosome doubling, adopt conventional method to cultivate and obtain double haploid DH plant;
(4) selfed seed on the results DH plant adopts gas chromatograph for determination fatty acid composition, selects the individual plant of high erucic acid, low linoleic acid and low linolenic to reserve seed for planting;
(5) in 2～3 generations of individual plant directive breeding, obtain at last that the FAE gene is normal, the homozygous lines of FAD2, FAD3 gene mutation, realizes the target of content of erucic acid 60%.
Advantage of the present invention is, this method will have high-oleic acid material now and combine with the high erucic acid material, create stable 60% non-transgenic high erucic acid material and the kind of being higher than of content of erucic acid, can be industrial erucic acid manufacturing enterprise quality raw materials are provided, reduce production costs, improve the business economic benefit and the market competitiveness.
Embodiment
The high rape material of common double of selecting content of erucic acid about 51 be maternal, is paternal hybrid with the high-oleic acid material of oleic acid content about 82, and acquisition F2 is for seed after the selfing generation; Get F2 and carry out half method gas chromatograph for determination, select 34 sowings of simple grain of high erucic acid, low linoleic acid and low linolenic for 200 in seed; Florescence gets flower pesticide and makes microspores culture, makes chromosome doubling with colchicin, cultivates to obtain 106 strains of double haploid (DH) plant; Selfed seed on the results DH plant adopts gas chromatograph for determination fatty acid composition, selects 22 individual plant sowings of high erucic acid, low linoleic acid and low linolenic, forms 22 strain systems; Each strain system selects 5 strain selfings, and selfed seed adopts the gas chromatograph analysis.Select three strain systems at last, fatty acid composition content shows unanimity between their individual plant, and the content of fatty acid of 5 single-strain seeds is content of erucic acid about 60%, oleic acid about 18%, linoleic acid about 9%, linolenic acid about 3%.Breed the high erucic acid new lines thus.
Claims (1)
1. the method for creating of a cabbage type rape high erucic acid material is characterized in that, realizes by following steps:
(1) it is maternal selecting the existing rape material of content of erucic acid more than 50%, is male parent with the rape mutant material of oleic acid content more than 80%, and hybridization obtains F1 for seed;
(2) plantation F1 is for plant, and F2 is for seed for results, and F2 is carried out half method gas chromatograph for determination for seed, selects the monoseeding of high erucic acid, low linoleic acid and low linolenic;
(3) florescence gets 3mm left and right sides flower pesticide and makes microspores culture, with microspore put into contain the 50mg/L colchicin the embryoid induction medium, in 32 ℃ incubator, handle 48h, finish chromosome doubling, adopt conventional method to cultivate and obtain double haploid DH plant;
(4) selfed seed on the results DH plant adopts gas chromatograph for determination fatty acid composition, selects the individual plant of high erucic acid, low linoleic acid and low linolenic to reserve seed for planting;
(5) in 2～3 generations of individual plant directive breeding, obtain at last that the FAE gene is normal, the homozygous lines of FAD2, FAD3 gene mutation, realizes the target of content of erucic acid 60%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101678907A CN101869049B (en) | 2010-05-11 | 2010-05-11 | Preparation method of cabbage type rape high erucic acid material |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101678907A CN101869049B (en) | 2010-05-11 | 2010-05-11 | Preparation method of cabbage type rape high erucic acid material |
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| Publication Number | Publication Date |
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| CN101869049A true CN101869049A (en) | 2010-10-27 |
| CN101869049B CN101869049B (en) | 2012-06-20 |
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| CN2010101678907A Expired - Fee Related CN101869049B (en) | 2010-05-11 | 2010-05-11 | Preparation method of cabbage type rape high erucic acid material |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875526A (en) * | 2012-12-20 | 2014-06-25 | 江苏省农业科学院 | Early stage breeding method of rape containing high content of oleic acid |
| CN104186307A (en) * | 2014-08-18 | 2014-12-10 | 安徽农科爱是福粮油有限公司 | Breeding method for cabbage type rape to be taken as vegetable and for pressing oil |
| CN106577258A (en) * | 2016-12-12 | 2017-04-26 | 福建农林大学 | Method for breeding special industrial high-resistance rape variety |
| CN106718823A (en) * | 2016-11-16 | 2017-05-31 | 西南大学 | A kind of method that utilization artificial synthesis Brassica napus prepare high oleic acid rape material |
| CN106718824A (en) * | 2016-11-16 | 2017-05-31 | 西南大学 | The method that high erucic acid cabbage type rape is prepared using artificial synthesis Brassica napus |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1436849A (en) * | 2002-02-09 | 2003-08-20 | 浙江绿洲农业股份有限公司 | Production method of transgenic rape used as erucic acid bioreactor |
| CN1888064A (en) * | 2006-07-25 | 2007-01-03 | 卢长明 | Gene engineering method of obtaining low erucic acid rape variety and its application |
| US20090036695A1 (en) * | 2001-10-12 | 2009-02-05 | Jeroen Alexander Wilmer | Oil Biosynthesis |
-
2010
- 2010-05-11 CN CN2010101678907A patent/CN101869049B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090036695A1 (en) * | 2001-10-12 | 2009-02-05 | Jeroen Alexander Wilmer | Oil Biosynthesis |
| CN1436849A (en) * | 2002-02-09 | 2003-08-20 | 浙江绿洲农业股份有限公司 | Production method of transgenic rape used as erucic acid bioreactor |
| CN1888064A (en) * | 2006-07-25 | 2007-01-03 | 卢长明 | Gene engineering method of obtaining low erucic acid rape variety and its application |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875526A (en) * | 2012-12-20 | 2014-06-25 | 江苏省农业科学院 | Early stage breeding method of rape containing high content of oleic acid |
| CN104186307A (en) * | 2014-08-18 | 2014-12-10 | 安徽农科爱是福粮油有限公司 | Breeding method for cabbage type rape to be taken as vegetable and for pressing oil |
| CN106718823A (en) * | 2016-11-16 | 2017-05-31 | 西南大学 | A kind of method that utilization artificial synthesis Brassica napus prepare high oleic acid rape material |
| CN106718824A (en) * | 2016-11-16 | 2017-05-31 | 西南大学 | The method that high erucic acid cabbage type rape is prepared using artificial synthesis Brassica napus |
| CN106718823B (en) * | 2016-11-16 | 2019-04-23 | 西南大学 | A method of high oleic acid rape material is prepared using artificial synthesis Brassica napus |
| CN106577258A (en) * | 2016-12-12 | 2017-04-26 | 福建农林大学 | Method for breeding special industrial high-resistance rape variety |
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| Publication number | Publication date |
|---|---|
| CN101869049B (en) | 2012-06-20 |
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