CN103875526A - Early stage breeding method of rape containing high content of oleic acid - Google Patents
Early stage breeding method of rape containing high content of oleic acid Download PDFInfo
- Publication number
- CN103875526A CN103875526A CN201210558588.3A CN201210558588A CN103875526A CN 103875526 A CN103875526 A CN 103875526A CN 201210558588 A CN201210558588 A CN 201210558588A CN 103875526 A CN103875526 A CN 103875526A
- Authority
- CN
- China
- Prior art keywords
- oleic acid
- rape
- sample
- days
- acid content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to an early stage breeding method of rape containing a high content of oleic acid. The method comprises the following steps: material plantation, sample preparation, sample pretreatment, sample analysis and material screening. Planting materials are screened according to the content of oleic acid in blades of rape before blooming, and a material with the blades containing a high content of oleic content undergoes bagged selfing during the florescence, so seeds containing a content of oleic content can be selected in the early stage, thereby the workload and the blindness of florescence bagging are reduced, and the seed selection efficiency is improved. The method has the advantages of reasonable technology, strong practicality, production cost reduction, and suitableness for application and popularization.
Description
Technical field
The present invention relates to a kind of early stage breeding method with high oleic acid proterties rape.
Background technology
Rape is important oil plant and nectar source crop, and Chinese vegetable seed oil yield accounts for the more than 60% of national edible vegetable oil gross yield, is the first source of domestic edible vegetable oil.Rapeseed oil is rich in oleic acid, its oleic acid content is greater than 80% rapeseed oil nutritional quality and can matches in excellence or beauty with olive oil, rapeseed oil is also the important source material of biodiesel, is the reborn type energy of environment-friendly type, has become one of important goal of rapeseed quality improvement so improve oleic acid content.When current Rapeseed quality breeding, conventionally detect the content of oleic acid with the mature seed of results, after being rapeseed cultivation, in the time that next year, rape was bloomed, carry out a large amount of bagging selfings, during Deng seed maturity, cut the seed of bagging selfing, use gas Chromatographic Determination oleic acid content, thereby eliminate the low material of oleic acid content, the material that preferred oil acid content is high.Rape is cross pollinated plant, for guaranteeing that test material is not polluted by foreign pollen, need to carry out bagging selfing at the florescence, uncertain due to material oleic acid content, the blindness that has caused casing material to select, a large amount of baggings have been wasted huge manpower and materials, and choose seeds by the height of mature seed oleic acid content, delay breeding process, reduced the efficiency of breeding.
Summary of the invention
The object of the invention is to overcome the defect that prior art exists, a kind of early stage breeding method with high oleic acid proterties rape is provided.
The technical scheme that realizes the object of the invention is: a kind of early stage breeding method with high oleic acid proterties rape, comprises the following steps:
(1) material plantation;
(2) sample preparation;
(3) sample pre-treatments;
(4) sample analysis;
(5) material screening.
As optimization, described each step is as follows respectively:
(1) material plantation: rape is normally sowed;
(2) sample preparation: any one growthdevelopmental stage before rape is bloomed, get same position functional leaf, clean and dry, put into drying box and dry to constant weight, with the pulverizing of high speed Universalpulverizer;
(3) sample pre-treatments: take leaf powder in centrifuge tube, add benzinum, lixiviate under Ultrasonic Conditions, then add active carbon, vortex mixes rear high speed centrifugation, draw whole supernatants in centrifuge tube, add the methanol solution of potassium hydroxide, vortex mixes rear leaving standstill, add again distilled water to suspend along tube wall, after leaving standstill, draw suspension supernatant in trace bottle, trace bottle is positioned in sample bottle, prepare to carry out gas chromatographic analysis;
(4) sample analysis: carry out chromatography with gas chromatograph, analysis condition is that injection port adopts constant voltage mode, fid detector, temperature is 300 ℃, the purity nitrogen that carrier gas is 99.999%, area normalization is processed gas-chromatography spectrogram, obtains oleic acid content;
(5) material screening: according to the number of blade oleic acid content, the material that blade oleic acid content is low is eliminated to the material medium to blade oleic acid content, in a small amount of bagging selfing of florescence, the material high to blade oleic acid content, in whole bagging selfings of florescence, thereby obtains high-oleic acid material.
As optimization, described each step is as follows respectively:
(1) material plantation: rape is normally sowed, every kind of material 20 row, after neat seedling, every row stays seedling 20 strains.
(2) sample preparation: any one growthdevelopmental stage before rape is bloomed, all choose 2 of same position functional leafs, clean and dry, put into electric heating constant-temperature blowing drying box, first complete, 105 ℃ of temperature, times 30 min, cool to again 80 ℃ and dry to constant weight, pulverize with high speed Universalpulverizer, cross 80 mesh sieves;
(3) sample pre-treatments: take leaf powder 0.2g in 10ml centrifuge tube, add boiling range and be the benzinum 2ml of 60-90 ℃, at 45 ℃, lixiviate 30 min under the Ultrasonic Conditions of 50KHz, add 20mg active carbon, vortex mixes rear high speed centrifugation 5min, centrifugal force is 8000 × g, draw whole supernatants in 5ml centrifuge tube, add the methanol solution of the 0.4mol/L potassium hydroxide of 0.5ml, vortex mixes rear standing 1h, add again 2ml distilled water to suspend along tube wall, leave standstill 0.5h, draw suspension supernatant in 250 μ l trace bottles, trace bottle is placed in the sample bottle of 2.5ml, gas chromatographic analysis is carried out in preparation,
(4) sample analysis: carry out chromatography with Agilent 7890 gas chromatographs, chromatographic column is μ m × 0.25,15.0m × 250 μ m, auto injection, injector temperature is 250 ℃, sample size is 2 μ l, split ratio is 5:1, injection port adopts constant voltage mode, pressure is 70.74Kpa, flow velocity 30ml/min, dottle pin purge flow rate is 3.00ml/min, fid detector, temperature is 300 ℃, carrier gas is 99.999% High Purity Nitrogen, make-up gas flow is 30.00ml/min, when chromatography, chromatographic column flow is 1.0 ml/min, temperature programming, 180 ℃ retain 1.0min, 5 ℃/min is warming up to 215 ℃, 215 ℃ retain 1.0min, 3 ℃/min is warming up to 230 ℃, 230 ℃ retain 1min, area normalization is processed gas-chromatography spectrogram, obtain the relative amount of oleic acid,
(5) material screening: according to the number of blade oleic acid content, blade oleic acid content is eliminated lower than 2.00% material, the material that is 2.00% ~ 4.00% to blade oleic acid content, in a small amount of bagging selfing of florescence, to blade oleic acid content higher than 4.00% material, in whole bagging selfings of florescence, thereby obtain high-oleic acid material.
As optimization, the plantation of described step (1) material can adopt the mode of transplanting to cultivate material.
As optimization, seedling stage, during Wintering Period, period of seedling establishment, flower bud a kind of sedge phase and the florescence of the period of rape sampling after being divided into rape and emerging in described step (2) sample preparation.
As optimization, the preferably seedling stage of 70 days ~ 77 days, the 104 days ~ during Wintering Period of 114 days, the period of seedling establishment of 134 days ~ 141 days, florescence of flower bud a kind of sedge phase and 180 days ~ 187 days of 163 days ~ 170 days after rape is emerged rape sample time in described step (2) sample preparation.
The present invention has positive effect: according to the bloom proterties of oleic acid content in front vane of rape, planting material is screened, the material that blade oleic acid content is higher, in a large amount of bagging selfings of florescence, thereby just can select in early days the seed that oleic acid content is higher, not only reduce the blindness of workload and florescence bagging but also improved the efficiency of seed selection, the method technique is reasonable, practical, further reduce production cost, be applicable to application.
Embodiment
Below by embodiment, and in conjunction with subordinate list, technical scheme of the present invention is further described in detail.
Embodiment 1:
(1) material plantation: the material number of different oleic acid contents is C153, C154, C157, C158, C159, C161, C162, C163, C164, C165, C167, C168, C170, C171, C172, C173, C174, C175, C176 and C177, in normal sowing on September 30th, 2008, each material kind 20 row, after neat seedling, every row stays seedling 20 strains.
(2) sample preparation: the during Wintering Period in the seedling stage of 70 days, 104 days, the period of seedling establishment of 134 days, flower bud a kind of sedge phase of 163 days and the florescence of 180 days after rape is emerged respectively, choose 2 leaves of outermost layer in every kind of numbering, each period, clean and dry, put into electric heating constant-temperature blowing drying box, first complete, 105 ℃ of temperature, times 30 min, cool to again 80 ℃ and dry to constant weight, pulverize with high speed Universalpulverizer, cross 80 mesh sieves;
(3) sample pre-treatments: take leaf powder 0.2g in 10ml centrifuge tube, add boiling range and be the benzinum 2ml of 60-90 ℃, at 45 ℃, lixiviate 30 min under the Ultrasonic Conditions of 50KHz, add 20mg active carbon, vortex mixes rear high speed centrifugation 5min, centrifugal force is 8000 × g, draw whole supernatants in 5ml centrifuge tube, add the methanol solution of the 0.4mol/L potassium hydroxide of 0.5ml, vortex mixes rear standing 1h, add again 2ml distilled water to suspend along tube wall, leave standstill 0.5h, draw suspension supernatant in 250 μ l trace bottles, trace bottle is placed in the sample bottle of 2.5ml, gas chromatographic analysis is carried out in preparation,
(4) sample analysis: carry out chromatography with Agilent 7890 gas chromatographs, chromatographic column is μ m × 0.25,15.0m × 250 μ m, auto injection, injector temperature is 250 ℃, sample size is 2 μ l, split ratio is 5:1, injection port adopts constant voltage mode, pressure is 70.74Kpa, flow velocity 30ml/min, dottle pin purge flow rate is 3.00ml/min, fid detector, temperature is 300 ℃, carrier gas is 99.999% High Purity Nitrogen, make-up gas flow is 30.00ml/min, when chromatography, chromatographic column flow is 1.0 ml/min, temperature programming, 180 ℃ retain 1.0min, 5 ℃/min is warming up to 215 ℃, 215 ℃ retain 1.0min, 3 ℃/min is warming up to 230 ℃, 230 ℃ retain 1min, area normalization is processed gas-chromatography spectrogram, obtain the relative amount of oleic acid,
(5) material screening: according to the number of blade oleic acid relative amount, blade oleic acid content is eliminated lower than 2.00% material, the material florescence that is 2.00 ~ 4.00% to blade oleic acid content is carried out random a small amount of bagging selfing, blade oleic acid content is carried out to a large amount of bagging selfings higher than 4.00% material florescence, thereby obtain high-oleic acid material.2009 annual repeated tests once.
The rape leaf, seed oleic acid relative amount (%) and the correlation analysis that obtain different oleic acid contents of 2008-2010 year are as shown in the table, and all results are got two annual means:
Embodiment 2:
(1) material plantation: the material number of different oleic acid contents is ys1, ys2, ys3, ys4, ys5, ys6, ys7, ys8, ys9, ys10, ys11, ys12, ys13, ys14, ys15, ys16, ys17, ys18, ys19, ys20, ys21, ys22, ys23, ys24, ys25 and ys26, in normal sowing on September 23rd, 2010, transplanted to October 30, each material is planted 20 row, every row 20 strains.
(2) sample preparation: the during Wintering Period in the seedling stage of 77 days, 114 days, the period of seedling establishment of 141 days, flower bud a kind of sedge phase of 170 days and the florescence of 187 days after rape is emerged respectively, choose 3 leaves of outermost layer in every kind of numbering, each period, clean and dry, put into electric heating constant-temperature blowing drying box, first complete, 105 ℃ of temperature, times 30 min, cool to again 80 ℃ and dry to constant weight, pulverize with high speed Universalpulverizer, cross 80 mesh sieves;
(3) sample pre-treatments: take leaf powder 0.2g in 10ml centrifuge tube, add boiling range and be the benzinum 2ml of 60-90 ℃, at 45 ℃, lixiviate 30 min under the Ultrasonic Conditions of 50KHz, add 20mg active carbon, vortex mixes rear high speed centrifugation 5min, centrifugal force is 8000 × g, draw whole supernatants in 5ml centrifuge tube, add the methanol solution of the 0.4mol/L potassium hydroxide of 0.5ml, vortex mixes rear standing 1h, add again 2ml distilled water to suspend along tube wall, leave standstill 0.5h, draw suspension supernatant in 250 μ l trace bottles, trace bottle is placed in the sample bottle of 2.5ml, gas chromatographic analysis is carried out in preparation,
(4) sample analysis: carry out chromatography with Agilent 7890 gas chromatographs, chromatographic column is μ m × 0.25,15.0m × 250 μ m, auto injection, injector temperature is 250 ℃, sample size is 2 μ l, split ratio is 5:1, injection port adopts constant voltage mode, pressure is 70.74Kpa, flow velocity 30ml/min, dottle pin purge flow rate is 3.00ml/min, fid detector, temperature is 300 ℃, carrier gas is 99.999% High Purity Nitrogen, make-up gas flow is 30.00ml/min, when chromatography, chromatographic column flow is 1.0 ml/min, temperature programming, 180 ℃ retain 1.0min, 5 ℃/min is warming up to 215 ℃, 215 ℃ retain 1.0min, 3 ℃/min is warming up to 230 ℃, 230 ℃ retain 1min, area normalization is processed gas-chromatography spectrogram, obtain the relative amount of oleic acid,
(5) material screening: according to the number of blade oleic acid content, blade oleic acid content is eliminated lower than 2.00% material, the material florescence that is 2.00 ~ 4.00% to blade oleic acid content is carried out random a small amount of bagging selfing, blade oleic acid content is carried out to a large amount of bagging selfings higher than 4.00% material florescence, thereby obtain high-oleic acid material.
The rape leaf, seed oleic acid relative amount (%) and the correlation analysis that obtain different oleic acid contents of 2010-2011 year are as shown in the table:
According to interpretation of result, find that in growth of rape process Leaf, oleic acid relative amount reaches utmost point significance level with results seed oleic acid relative amount correlation all the time, be that in plant leaf blade, oleic acid relative amount is high, its results seed oleic acid relative amount is also high, in plant leaf, oleic acid content is low, and its results seed oil acid content is also low.Test Leaf oleic acid content is lower than 2.00% material, mature seed oleic acid content is between 62.63% ~ 67.82%, blade oleic acid content is 2.00% ~ 4.00% material, mature seed oleic acid content is between 70.11% ~ 79.05%, the material that blade oleic acid content is greater than 4.00%, mature seed oleic acid content is between 80.64% ~ 86.11%.
According to the bloom proterties of oleic acid content in front vane of rape, planting material is screened, the material that blade oleic acid content is higher, in a large amount of bagging selfings of florescence, thereby just can select in early days the seed that oleic acid content is higher, not only reduce the blindness of workload and florescence bagging but also improved the efficiency of seed selection, the method technique is reasonable, practical, further reduce production cost.
Above-described specific embodiment; object of the present invention, technical scheme and beneficial effect are further described; institute is understood that; the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any modification of making, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (6)
1. an early stage breeding method with high oleic acid proterties rape, is characterized in that: comprise the following steps:
(1) material plantation;
(2) sample preparation;
(3) sample pre-treatments;
(4) sample analysis;
(5) material screening.
2. a kind of early stage breeding method with high oleic acid proterties rape according to claim 1, is characterized in that: described each step is as follows respectively:
(1) material plantation: rape is normally sowed;
(2) sample preparation: any one growthdevelopmental stage before rape is bloomed, get same position functional leaf, clean and dry, put into drying box and dry to constant weight, with the pulverizing of high speed Universalpulverizer;
(3) sample pre-treatments: take leaf powder in centrifuge tube, add benzinum, lixiviate under Ultrasonic Conditions, then add active carbon, vortex mixes rear high speed centrifugation, draw whole supernatants in centrifuge tube, add the methanol solution of potassium hydroxide, vortex mixes rear leaving standstill, add again distilled water to suspend along tube wall, after leaving standstill, draw suspension supernatant in trace bottle, trace bottle is positioned in sample bottle, prepare to carry out gas chromatographic analysis;
(4) sample analysis: carry out chromatography with gas chromatograph, analysis condition is that injection port adopts constant voltage mode, fid detector, temperature is 300 ℃, the purity nitrogen that carrier gas is 99.999%, area normalization is processed gas-chromatography spectrogram, obtains oleic acid content;
(5) material screening: according to the number of blade oleic acid content, the material that blade oleic acid content is low is eliminated to the material medium to blade oleic acid content, in a small amount of bagging selfing of florescence, the material high to blade oleic acid content, in whole bagging selfings of florescence, thereby obtains high-oleic acid material.
3. a kind of early stage breeding method with high oleic acid proterties rape according to claim 2, is characterized in that: described each step is as follows respectively:
(1) material plantation: rape is normally sowed, every kind of material 20 row, after neat seedling, every row stays seedling 20 strains.
(2) sample preparation: any one growthdevelopmental stage before rape is bloomed, all choose 2 of same position functional leafs, clean and dry, put into electric heating constant-temperature blowing drying box, first complete, 105 ℃ of temperature, times 30 min, cool to again 80 ℃ and dry to constant weight, pulverize with high speed Universalpulverizer, cross 80 mesh sieves;
(3) sample pre-treatments: take leaf powder 0.2g in 10ml centrifuge tube, add boiling range and be the benzinum 2ml of 60-90 ℃, at 45 ℃, lixiviate 30 min under the Ultrasonic Conditions of 50KHz, add 20mg active carbon, vortex mixes rear high speed centrifugation 5min, centrifugal force is 8000 × g, draw whole supernatants in 5ml centrifuge tube, add the methanol solution of the 0.4mol/L potassium hydroxide of 0.5ml, vortex mixes rear standing 1h, add again 2ml distilled water to suspend along tube wall, leave standstill 0.5h, draw suspension supernatant in 250 μ l trace bottles, trace bottle is placed in the sample bottle of 2.5ml, gas chromatographic analysis is carried out in preparation,
(4) sample analysis: carry out chromatography with Agilent 7890 gas chromatographs, chromatographic column is μ m × 0.25,15.0m × 250 μ m, auto injection, injector temperature is 250 ℃, sample size is 2 μ l, split ratio is 5:1, injection port adopts constant voltage mode, pressure is 70.74Kpa, flow velocity 30ml/min, dottle pin purge flow rate is 3.00ml/min, fid detector, temperature is 300 ℃, carrier gas is 99.999% High Purity Nitrogen, make-up gas flow is 30.00ml/min, when chromatography, chromatographic column flow is 1.0 ml/min, temperature programming, 180 ℃ retain 1.0min, 5 ℃/min is warming up to 215 ℃, 215 ℃ retain 1.0min, 3 ℃/min is warming up to 230 ℃, 230 ℃ retain 1min, area normalization is processed gas-chromatography spectrogram, obtain the relative amount of oleic acid,
(5) material screening: according to the number of blade oleic acid content, blade oleic acid content is eliminated lower than 2.00% material, the material that is 2.00% ~ 4.00% to blade oleic acid content, in a small amount of bagging selfing of florescence, to blade oleic acid content higher than 4.00% material, in whole bagging selfings of florescence, thereby obtain high-oleic acid material.
4. according to a kind of early stage breeding method with high oleic acid proterties rape described in any one in claims 1 to 3, it is characterized in that: the plantation of described step (1) material can adopt the mode of transplanting to cultivate material.
5. according to a kind of early stage breeding method with high oleic acid proterties rape described in any one in claims 1 to 3, it is characterized in that: seedling stage, during Wintering Period, period of seedling establishment, flower bud a kind of sedge phase and the florescence of the period of rape sampling after being divided into rape and emerging in described step (2) sample preparation.
6. a kind of early stage breeding method with high oleic acid proterties rape according to claim 5, is characterized in that: the preferably seedling stage of 70 days ~ 77 days, the 104 days ~ during Wintering Period of 114 days, the period of seedling establishment of 134 days ~ 141 days, florescence of flower bud a kind of sedge phase and 180 days ~ 187 days of 163 days ~ 170 days after rape is emerged rape sample time in described step (2) sample preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210558588.3A CN103875526A (en) | 2012-12-20 | 2012-12-20 | Early stage breeding method of rape containing high content of oleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210558588.3A CN103875526A (en) | 2012-12-20 | 2012-12-20 | Early stage breeding method of rape containing high content of oleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103875526A true CN103875526A (en) | 2014-06-25 |
Family
ID=50944919
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210558588.3A Pending CN103875526A (en) | 2012-12-20 | 2012-12-20 | Early stage breeding method of rape containing high content of oleic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103875526A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050039233A1 (en) * | 2002-05-15 | 2005-02-17 | Saskatchewan Wheat Pool | High oleic acid Brassica juncea |
| CN101356278A (en) * | 2005-11-07 | 2009-01-28 | 马克思-普朗克科学促进协会公司 | Method for increasing the total oil content in oil plants |
| CN101869049A (en) * | 2010-05-11 | 2010-10-27 | 西南大学 | Preparation method of cabbage type rape high erucic acid material |
| CN102057865A (en) * | 2010-10-20 | 2011-05-18 | 江苏省农业科学院 | Method for creating new high oleic acid germplasm by treating rape germinating seeds with low-dose <60>Co-gamma rays |
-
2012
- 2012-12-20 CN CN201210558588.3A patent/CN103875526A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050039233A1 (en) * | 2002-05-15 | 2005-02-17 | Saskatchewan Wheat Pool | High oleic acid Brassica juncea |
| CN101356278A (en) * | 2005-11-07 | 2009-01-28 | 马克思-普朗克科学促进协会公司 | Method for increasing the total oil content in oil plants |
| CN101869049A (en) * | 2010-05-11 | 2010-10-27 | 西南大学 | Preparation method of cabbage type rape high erucic acid material |
| CN102057865A (en) * | 2010-10-20 | 2011-05-18 | 江苏省农业科学院 | Method for creating new high oleic acid germplasm by treating rape germinating seeds with low-dose <60>Co-gamma rays |
Non-Patent Citations (3)
| Title |
|---|
| GANG XIAO,ET AL.: "Identification of differentially expressed genes in seeds of two Brassica napus mutant lines with different oleic acid content", 《AFRICAN JOURNAL OF BIOTECHNOLOGY》 * |
| 官梅等: "高油酸油菜品系农艺性状研究", 《中国油料作物学报》 * |
| 高建芹等: "应用气相色谱仪分析油菜脂肪酸含量", 《江苏农业学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102613086A (en) | Hormone-free tissue culture method for dendrobium candidum | |
| CN103087975A (en) | Apocarya pollen in-vitro germination liquid culture medium and application thereof in measuring pollen activity | |
| Du et al. | Enhanced growth and lipid production of Chlorella pyrenoidosa by plant growth regulator GA3 | |
| CN103109740A (en) | New strain breeding method of high-quality purple perilla | |
| CN110250211B (en) | Biophysical method for inducing aquilaria sinensis to knot fragrance and application thereof | |
| CN107333651A (en) | A kind of method for improving Dendrobidium huoshanness protocorm alkaloid | |
| Mu et al. | Alkaloid accumulation in different parts and ages of Lycoris chinensis | |
| CN106290637B (en) | A kind of polycyclic aromatic hydrocarbon enters the method for inspection of cabbage heart main path | |
| CN103875526A (en) | Early stage breeding method of rape containing high content of oleic acid | |
| Węglarz et al. | Intraspecific variability of wild marjoram (Origanum vulgare L.) naturally occurring in Poland | |
| Yang et al. | Analysis of floral scent emitted from Syringa plants | |
| Afsharypuor et al. | Glucosinolate degradation products, alkanes and fatty acids from plants and cell cultures of Descurainia sophia | |
| Wang et al. | Seed oil content and fatty acid composition of annual halophyte Suaeda acuminata: A comparative study on dimorphic seeds | |
| US20200022325A1 (en) | Phytohormone enriched microalgae methods and compositions | |
| Rahangdale et al. | Provenance variation for oil content and fatty acid composition in seed of Pongamia pinnata (L.) Pierre | |
| CN101392234B (en) | Method for producing valerian iridoids | |
| CN109906941B (en) | Psammosilene tunicoides adventitious root suspension culture method | |
| Qi et al. | Effects of Plant Density on the Economic Yield and Agronomic Characters of Perilla frutescens L. | |
| Calica | Nodulation and nitrogen fixation of Pongamia pinnata | |
| CN107200684A (en) | A kind of method that myristic acid is extracted in the ballstone algae from ocean | |
| CN106831696A (en) | Derivative of macrolides and its preparation method and application | |
| Karaman et al. | High yielding indigo sources in native Isatis (Brassicaceae) taxa from Turkey | |
| CN109468359A (en) | A kind of ginsenoside Rk6Preparation method | |
| Zhang et al. | Effect of cultivation methods on seed yield traits, oil yield, and fatty acid compositions of cultivated Paeonia ostii | |
| CN104450528A (en) | Method for isolating and screening endophytic fungi from gardenia jasminoides |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140625 |
|
| RJ01 | Rejection of invention patent application after publication |