CN106577256A - Breeding method for rapidly introducing rice target characters - Google Patents
Breeding method for rapidly introducing rice target characters Download PDFInfo
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- CN106577256A CN106577256A CN201611137394.0A CN201611137394A CN106577256A CN 106577256 A CN106577256 A CN 106577256A CN 201611137394 A CN201611137394 A CN 201611137394A CN 106577256 A CN106577256 A CN 106577256A
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- parent
- bc1f1
- recurrent parent
- target characters
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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Abstract
The invention discloses a breeding method for rapidly introducing rice target characters. The method includes the following steps that rice resources carrying target characters serve as a male parent or a female parent and are hybridized with a recurrent parent to obtain a hybrid F1; the recurrent parent serves as a female parent to be hybridized with the hybrid F1 to obtain a BC1F1 group; according to 48 molecular markers distributed evenly on 12 chromosomes of rice in the SSR labeling method of a rice variety identification technical regulation, genetic similarity analysis is conducted on a single plant of the BC1F1 group, and a BC1F1 single plant is screened, wherein the BC1F1 single plant carries the target characters and the genetic similarity is higher than 90% compared with the recurrent parent; the recurrent parent serves as a female parent to be hybridized with the BC1F1 single plant, and a plant carrying the target characters and similar to the recurrent parent is bred and selected from later generations. Through the comparison of SSR molecular markers with the recurrent parent, when the genetic similarity is high, the target characters are introduced rapidly and more-efficiently, and the genetic resource innovative breeding speed is greatly increased.
Description
Technical field
The present invention relates to rice breeding field, and in particular to a kind of selection for quickly introducing Oryza sativa L. objective trait.
Background technology
Introduced into Rice objective trait, typically adopts pedigree method at present, need to be by many for backcross breeding.Choose in F1 generation true
Hybrid is returned with recurrent parent, is obtained BC1F1, then through many generation (4 is more than generation) backcrossings, is obtained advanced clones；Again from BC5F2
Rise, selection-breeding carries objective trait the strain similar to parent；Selection-breeding cycle length, workload are big, become Rice Germplasm Resources wound
New bottleneck.
The content of the invention
To solve the above problems, the invention provides a kind of selection for quickly introducing Oryza sativa L. objective trait, by SSR
Molecular marker compares importing objective trait rapidly and efficiently in the case of genetic similarty height with recurrent parent, greatly accelerates kind
The selection-breeding speed of matter resource innovation.
For achieving the above object, the technical scheme taken of the present invention is：
A kind of selection for quickly introducing Oryza sativa L. objective trait, comprises the steps：
S1, to carry the rice pest insects of objective trait as male parent or female parent, hybridize with recurrent parent, obtain hybridizing F1；So
Afterwards using recurrent parent as female parent, hybridized with hybridization F1, obtained BC1F1 colonies；
S2,12 chromosomes of Oryza sativa L. are uniformly distributed in using 48 in rice varieties identification technology code SSR marker method
On molecular marker carry out genetic similarity analysis to the BC1F1 colonies individual plant obtained by step S1, screening carries objective trait base
Because and compare with recurrent parent genetic similarty higher than 90% BC1F1 individual plants；
S3, with recurrent parent as female parent, be returned with the BC1F1 individual plants obtained by step S2, select from offspring and take
With objective trait and the strain similar to recurrent parent.
The invention has the advantages that：
Importing targeted rapidly and efficiently in the case of genetic similarty height is compared by SSR molecular marker with recurrent parent
Shape, greatly accelerates the selection-breeding speed of Germplasm enhancement.
Description of the drawings
Fig. 1 is a kind of flow chart of the selection for quickly introducing Oryza sativa L. objective trait of the embodiment of the present invention.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, with reference to embodiments the present invention is carried out further
Describe in detail.It should be appreciated that specific embodiment described herein is not used to limit this only to explain the present invention
It is bright.
As shown in figure 1, a kind of selection for quickly introducing Oryza sativa L. objective trait is embodiments provided, including such as
Lower step：
S1, to carry the rice pest insects of objective trait as male parent or female parent, hybridize with recurrent parent, obtain hybridizing F1；So
Afterwards using recurrent parent as female parent, hybridized with hybridization F1, obtained BC1F1 colonies；
S2,12 chromosomes of Oryza sativa L. are uniformly distributed in using 48 in rice varieties identification technology code SSR marker method
On molecular marker carry out genetic similarity analysis to the BC1F1 colonies individual plant obtained by step S1, screening carries objective trait base
Because and compare with recurrent parent genetic similarty higher than 90% BC1F1 individual plants；
S3, with recurrent parent as female parent, be returned with the BC1F1 individual plants obtained by step S2, select from offspring and take
With objective trait and the strain similar to recurrent parent.
Embodiment
Breeding goal：Purple endosperm Black seed coat character is imported in 93-11 restorers.
Breeding Process：1) to carry the purple endosperm (this seminar resource) of Black seed coat character as female parent, with recurrent parent
93-11 hybridizes, and obtains hybridizing F1；Using recurrent parent 93-11 as female parent, hybridized with Hybrids F1, obtained BC1F1 colonies；
2) 48 molecule marks being uniformly distributed on 12 chromosomes of Oryza sativa L. in " rice varieties identification technology code SSR marker method " are utilized
Note carries out genetic similarity analysis to BC1F1 colonies individual plant, and screening carries objective trait gene and compares genetic resemblance with 93-11
6 plants of the BC1F1 individual plants higher than 90% are spent, strain is respectively：1st, 2,5,6,7,10, such as table 1；(3) with 93-11 as female parent, with choosing
The BC1F1 individual plants selected are returned, and strain with Black seed coat character and similar to 93-11 economic characters is selected from offspring
System.
1. genetic similarity analytical table of table
Table is noted：Strain 1-18 in table, primer 48 pairs, 1 representative are identical with 93-11 bands, and 0 representative is different from 93-11 bands,
Each strain score value is calculated, score value is more than 43 i.e. with 93-11 similarities higher than 90%.
Originally it is embodied as changing the patterns of many generation (more than the BC5F2) selection-breeding of Introduced into Rice objective trait needs backcrossing；Utilize
Target strain in SSR marker screening BC1F1 offsprings with recurrent parent similarity more than more than 90%, with recurrent parent as female parent
It is returned with the target strain of screening, carrying objective trait the strain similar to recurrent parent, contracting is selected from BC2F1
Short selection-breeding process, workload is alleviated, practicality is very strong.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (1)
1. a kind of selection for quickly introducing Oryza sativa L. objective trait, it is characterised in that comprise the steps：
S1, to carry the rice pest insects of objective trait as male parent or female parent, hybridize with recurrent parent, obtain hybridizing F1；Then with
Recurrent parent is hybridized with hybridization F1, is obtained BC1F1 colonies as female parent；
S2, it is uniformly distributed on 12 chromosomes of Oryza sativa L. using 48 in rice varieties identification technology code SSR marker method
Molecular marker carries out genetic similarity analysis to the BC1F1 colonies individual plant obtained by step S1, screening carry objective trait gene and
Compare BC1F1 individual plant of the genetic similarty higher than 90% with recurrent parent；
S3, with recurrent parent as female parent, be returned with the BC1F1 individual plants obtained by step S2, carrying mesh selected from offspring
Mark character the strain similar to recurrent parent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201611137394.0A CN106577256A (en) | 2016-12-03 | 2016-12-03 | Breeding method for rapidly introducing rice target characters |
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| CN201611137394.0A CN106577256A (en) | 2016-12-03 | 2016-12-03 | Breeding method for rapidly introducing rice target characters |
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| CN106577256A true CN106577256A (en) | 2017-04-26 |
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| CN201611137394.0A Pending CN106577256A (en) | 2016-12-03 | 2016-12-03 | Breeding method for rapidly introducing rice target characters |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115226625A (en) * | 2022-07-18 | 2022-10-25 | 商丘市农林科学院 | Breeding method of sorghum restorer line |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1124565A (en) * | 1995-06-21 | 1996-06-19 | 四川省农业科学院 | Dynamic multi-linear breeding process for resisting rice blast of hybridized rice |
| CN1429479A (en) * | 2001-12-30 | 2003-07-16 | 福建农林大学 | Breeding method of large embryo rice variety |
| CN102511385A (en) * | 2011-12-21 | 2012-06-27 | 中国农业科学院作物科学研究所 | Selection method of polymeric rice sheath blight resistance breeding material |
| CN105557502A (en) * | 2015-12-16 | 2016-05-11 | 中国农业科学院深圳生物育种创新研究院 | Method for rapidly stabilizing rice backcross introgression population trait |
| CN105684883A (en) * | 2016-01-13 | 2016-06-22 | 国家粳稻工程技术研究中心 | Method for rapidly and selectively culturing cold-resistant high-quality king rice variety in rice growing region of Heilongjiang |
-
2016
- 2016-12-03 CN CN201611137394.0A patent/CN106577256A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1124565A (en) * | 1995-06-21 | 1996-06-19 | 四川省农业科学院 | Dynamic multi-linear breeding process for resisting rice blast of hybridized rice |
| CN1429479A (en) * | 2001-12-30 | 2003-07-16 | 福建农林大学 | Breeding method of large embryo rice variety |
| CN102511385A (en) * | 2011-12-21 | 2012-06-27 | 中国农业科学院作物科学研究所 | Selection method of polymeric rice sheath blight resistance breeding material |
| CN105557502A (en) * | 2015-12-16 | 2016-05-11 | 中国农业科学院深圳生物育种创新研究院 | Method for rapidly stabilizing rice backcross introgression population trait |
| CN105684883A (en) * | 2016-01-13 | 2016-06-22 | 国家粳稻工程技术研究中心 | Method for rapidly and selectively culturing cold-resistant high-quality king rice variety in rice growing region of Heilongjiang |
Non-Patent Citations (2)
| Title |
|---|
| 徐福荣等: "基于表型性状和SSR 分子标记的云南省水稻主要育成品种(系) 的遗传相似性分析", 《植物遗传资源学报》 * |
| 陈氏秋江: "水稻籽粒性状的SSR关联分析", 《中国水稻科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115226625A (en) * | 2022-07-18 | 2022-10-25 | 商丘市农林科学院 | Breeding method of sorghum restorer line |
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Application publication date: 20170426 |