CN107201403A - Cotton fiber length correlation QTL and its application - Google Patents
Cotton fiber length correlation QTL and its application Download PDFInfo
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- CN107201403A CN107201403A CN201710434758.XA CN201710434758A CN107201403A CN 107201403 A CN107201403 A CN 107201403A CN 201710434758 A CN201710434758 A CN 201710434758A CN 107201403 A CN107201403 A CN 107201403A
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Abstract
The present invention relates to plant genetic field, and in particular to cotton fiber length correlation QTL and its application.The cotton fiber length correlation QTL qUHM 11 are located at No. 1 chromosome, and its side neighbour's mark is BNL1667, and cotton fiber length correlation QTL qUHM 22 1 are located at No. 22 chromosomes, and side neighbour's mark is CICR345.The fibre length correlation QTL of the present invention, is detected in two environment, with the stability independent of environment.
Description
Technical field
The present invention relates to plant genetic field, and in particular to cotton fiber length correlation QTL and its application.
Background technology
Quantitative trait locus (Quantitative trait locus, QTL) is to be located on chromosome to influence quantitative
Some regions of shape, a quantitative character often by multiple diverse locations for being distributed in genome quantitative trait locus shadow
Ring.Up to the present, QTL positioning is carried out by building segregating population and correlation fractal dimension based on biparent cross.But
Current low-density genetic map and interim separation group cause the QTL detected accuracy and stability than in ideal
It is low, and few cotton fiber quality correlation QTL that can be applied to Molecular breeding in upland cotton
The content of the invention
It is an object of the invention to provide cotton fiber length correlation QTL.
Another object of the present invention is above-mentioned cotton fiber length correlation QTL specific SSR primers.
According to the cotton fiber length correlation QTL qUHM-1-1 of the present invention, it is located at No. 1 chromosome, its side neighbour marks
BNL3580-BNL1667。
According to the cotton fiber length correlation QTL qUHM-22-1 of the present invention, it is located at No. 22 chromosomes, side neighbour marks
NAU3093-CICR345。
It is according to the sequence of the cotton fiber length correlation QTL qUHM-1-1 of the present invention specific SSR primers：
BNL3580F：5'CTTGTTTACATTCCCTTCTTTATACC 3'；
BNL3580R:5'CAAAGGCGAACTCTTCCAAA 3'；
BNL1667F:5'AGGTGCTTCAGGCATGATTC 3'；
BNL1667R:5'CCCTCACACCTAAACCCAAA 3'。
It is according to the sequence of the cotton fiber length correlation QTL qUHM-22-1 of the present invention specific SSR primers：
NAU3093F：5'GATGGGCAGAGGCTACTTTG 3'
NAU3093R：5'GATGGGCAGAGGCTACTTTG 3'
CICR0345-F：5'GATGGGCAGAGGCTACTTTG 3'
CICR0345-R：5'GATGGGCAGAGGCTACTTTG 3'.
According to the embodiment of the present invention, with upland cotton PD94042 (P1) for receptor parent, with yellowish-brown cotton
(G.mustelinum)(P2) hybridize for donor parents, and with upland cotton PD94042 (P1) it is recurrent parent third backcross generation, then from
Hand over three generations to build backcrossing advanced lines colony, therefrom select the introgressive line of a set of covering cotton full-length genome, altogether 65 parts of materials,
Carry out backcrossing advanced lines qtl analysis (AB-QTL).The analysis of introgressive line population data includes：
(1) genotype data is counted
Record the type of parent and filial generation SSR marker：With parent (P1) identical homozygosis banding pattern is designated as " 1 ", with parent (P2)
Identical homozygosis banding pattern is designated as " 2 ", and the heterozygosis banding pattern of two parents is designated as " 3 ", and fuzzy or missing data is designated as " 0 "；Work as parent
(P1) it is recessiveness, parent (P2) for it is dominant when, P1Banding pattern is designated as " 1 ", P2、F1Banding pattern is designated as " 4 ", as parent (P1) it is dominant, parent
This (P2) for it is recessive when, P2Banding pattern is designated as " 2 ", P1、F1Banding pattern is designated as " 5 ".
(2) phenotypic data is counted
3 years fiber quality phenotypic datas are subjected to the statistical analyses such as average value, scope, the coefficient of variation, the degree of bias, and will
The phenotypic data of introgressive line makes column distribution map.
(3) genetic map is built to position with QTL
The genotype data of comprehensive introgressive line colony, with the corresponding sequence of each molecular labeling, compares Tetraploid G. hirsutum base
Because of a group database, the genomic locations of each molecular labeling are obtained, as reference information, the linkage relationship mark is divided
Analysis, builds the genetic linkage mapses of molecular labeling.The genotype data of fiber quality is combined with phenotypic data again, carried out fine
Dimension quality QTL finely positionings are simultaneously analyzed result, and the minimum LOD values of detection QTL are 2.0, finally to target QTL in linkage map
Position in spectrum or chromosome.
It is located at No. 1 and No. 22 dyeing respectively according to fibre length correlation the QTL qUHM-1-1 and qUHM-22-1 of the present invention
On body (such as Fig. 2), and contribution rate is larger, and its beneficial gene is all from yellowish-brown cotton.
Compared with the stable QTL of existing fibre length, the invention provides be located at No. 1 and No. 22 chromosome of cotton respectively
Fibre length correlation QTL, is detected in two environment, with the stability independent of environment.
Brief description of the drawings
Fig. 1 shows yellowish-brown introgressive line fibre strength；
Fig. 2 shows the genetic map of No. 1 and No. 22 chromosome built based on yellowish-brown cotton introgressive line；
Fig. 3 shows two stable fibre length main effect QTLs, wherein, A groups are that fibre length QTL qUHM-1-1, B groups are
Fibre length QTL qUHM-22-1.
Fig. 4 shows the NIL selected based on fibre length QTL qUHM-1-1 and qUHM-22-1, and light gray is represented
Pure and mild upland cotton genotype, black represents yellowish-brown cotton gene introgression.
Embodiment
Embodiment 1
With upland cotton PD94042 (P1) for receptor parent, with yellowish-brown cotton (G.mustelinum) (P2) it is that donor parents are miscellaneous
Hand over, and with upland cotton PD94042 (P1) backcrossing advanced lines colony is built for recurrent parent third backcross generation, then selfing three generations, therefrom
The introgressive line of a set of covering cotton full-length genome is selected, altogether 65 parts of materials, carry out backcrossing advanced lines qtl analysis.Extract cotton
Flower leaf DNA, carries out SSR molecular marker.The primer between PD94042 and yellowish-brown cotton with polymorphism is filtered out, to yellowish-brown cotton
Introgressive line colony is scanned and qtl analysis.
1. introgressive line population data is analyzed
(1) genotype data is counted
Record the type of parent and filial generation SSR marker：With parent (P1) identical homozygosis banding pattern is designated as " 1 ", with parent (P2)
Identical homozygosis banding pattern is designated as " 2 ", and the heterozygosis banding pattern of two parents is designated as " 3 ", and fuzzy or missing data is designated as " 0 "；Work as parent
(P1) it is recessiveness, parent (P2) for it is dominant when, P1Banding pattern is designated as " 1 ", P2、F1Banding pattern is designated as " 4 ", as parent (P1) it is dominant, parent
This (P2) for it is recessive when, P2Banding pattern is designated as " 2 ", P1、F1Banding pattern is designated as " 5 ".
(2) phenotypic data is counted
3 years fiber quality phenotypic datas are subjected to the statistical analyses such as average value, scope, the coefficient of variation, the degree of bias, and will
The phenotypic data of introgressive line makes column distribution map.
(3) genetic map is built to position with QTL
The genotype data of comprehensive introgressive line colony, with the corresponding sequence of each molecular labeling, compares Tetraploid G. hirsutum base
Because of a group database, the genomic locations of each molecular labeling are obtained, as reference information, the linkage relationship mark is divided
Analysis, builds the genetic linkage mapses of molecular labeling.The genotype data of fiber quality is combined with phenotypic data again, carried out fine
Dimension quality QTL finely positionings are simultaneously analyzed result, and the minimum LOD values of detection QTL are 2.0, finally to target QTL in linkage map
Position in spectrum or chromosome.
28 fibre length QTL are detected altogether, and 18 chromosomes are covered altogether.It can be seen from additive effect, 6 QTL's
Favorable allels come from upland cotton PD94042, and remaining 22 QTL favorable allels are all from yellowish-brown cotton.Big portion
The fibre length QTL divided is only detected in an environment, but 3 QTL are detected for continuous 2 years, qUHM-1-1, qUHM-
2 years marker intervals of 22-1 are all same (Fig. 3), illustrate that they have the stability independent of environment.
It is located at No. 1 and No. 22 dyeing respectively according to fibre length correlation the QTL qUHM-1-1 and qUHM-22-1 of the present invention
On body (such as Fig. 2), and contribution rate is larger, and its beneficial gene is all from yellowish-brown cotton.
The fibre length QTL of table 2.6
Note：Y1 represents First Year；Y2 represents Second Year, and both represent different environment (similarly hereinafter)
Based on the fibre length main effect QTL on No. 1 chromosome：QUHM-1-1, establishes a set of NIL (Fig. 4),
IL6, IL9, IL15, IL17 this 4 excellent introgressive lines are contained, between the adjacent marker site BNL3580-BNL1667 in side all
Or most of is yellowish-brown cotton introgressive gene, remaining position is almost upland cotton gene.
And based on fibre length main effect QTL on No. 22 chromosomes：A set of NIL (Fig. 4) that qUHM-22-1 is set up,
Equally contain 4 excellent introgressive lines, respectively IL6, IL9, IL11, IL17.As seen from the figure, in qUHM-22-1 side neighbour's mark
Remember NAU3093-CICR345 between, half is yellowish-brown cotton introgressive gene, moreover, mark NAU5046 around, IL9, IL11,
IL17 gradually oozes fragment in the presence of one section of yellowish-brown cotton.
In summary, this 5 introgressive lines have yellowish-brown cotton gene around fibre length main effect QTL, show long with fiber
The beneficial gene of degree is related.
The present invention has obtained two fibre length main effect QTLs：QUHM-1-1, qUHM-22-1, and integrated fiber length
Phenotypic data, obtains 5 introgressive line kinds of the fibre length between 32-34mm：IL6、IL9、IL11、IL15、IL17.It is based on
Fibre length main effect QTL on No. 1 chromosome：QUHM-1-1, establishes a set of NIL (Fig. 4), contain IL6,
This 4 excellent introgressive lines of IL9, IL15, IL17, be wholly or largely between the adjacent marker site BNL3580-BNL1667 in side
Yellowish-brown cotton introgressive gene, remaining position is almost upland cotton gene.And based on fibre length main effect QTL on No. 22 chromosomes：
A set of NIL (Fig. 4) that qUHM-22-1 is set up, equally contains 4 excellent introgressive lines, respectively IL6, IL9,
IL11、IL17.As seen from the figure, between qUHM-22-1 side neighbour's mark NAU3093-CICR345, half is that yellowish-brown cotton gradually oozes base
Cause, moreover, around mark NAU5046, IL9, IL11, IL17 gradually ooze fragment in the presence of one section of yellowish-brown cotton.In summary,
This 5 introgressive lines have yellowish-brown cotton gene around fibre length main effect QTL, show may have with the beneficial gene of fibre length
Very big association.Yellowish-brown cotton gene is gradually oozed knowable to situation on comprehensive No. 1 and No. 22 chromosomes, introgressive line IL6, and IL9, IL17 exists
All there is the related yellowish-brown cotton gene of fibre length on two chromosomes, can as follow-up study prioritizing selection material.
Embodiment 2
With upland cotton PD94042 (P1) for receptor parent, with yellowish-brown cotton (G.mustelinum) (P2) it is that donor parents are miscellaneous
Hand over, and with upland cotton PD94042 (P1) backcrossing advanced lines colony is built for recurrent parent third backcross generation, then selfing three generations, therefrom
The introgressive line of a set of covering cotton full-length genome is selected, backcrossing advanced lines qtl analysis is carried out.Cotton leaf DNA is extracted, is utilized
Primer shown in following table carries out SSR molecular marker, and SSR Molecular Detections result unanimously, is said with the genotype data statistics in embodiment 1
Primer shown in bright table 1 is the cotton fiber length correlation QTL of the present invention specific SSR primers.
The specificity SSR primers of table 1
| Primer | Primer sequence ((5'-3')) |
| BNL3580F | CTTGTTTACATTCCCTTCTTTATACC |
| BNL3580R | CAAAGGCGAACTCTTCCAAA |
| BNL1667F | AGGTGCTTCAGGCATGATTC |
| BNL1667R | CCCTCACACCTAAACCCAAA |
| NAU3093F | GATGGGCAGAGGCTACTTTG |
| NAU3093R | AGCACAGGAGCAAAGGAAAA |
| CICR0345-F | CTAAGACCGAGTGGCTTGAT |
| CICR0345-R | AGCCAAATCCAGTAAACAGC |
<110>Nantong University
<120>Cotton fiber length correlation QTL and its application
<160>8
<210> 1
<211> 26
<212> DNA
<213>Artificial sequence
<400> 1
cttgtttaca ttcccttctt tatacc 26
<210> 2
<211> 20
<212> DNA
<213>Artificial sequence
<400> 2
caaaggcgaa ctcttccaaa 20
<210> 3
<211> 20
<212> DNA
<213>Artificial sequence
<400> 3
aggtgcttca ggcatgattc 20
<210> 4
<211> 20
<212> DNA
<213>Artificial sequence
<400> 4
ccctcacacc taaacccaaa 20
<210> 5
<211> 20
<212> DNA
<213>Artificial sequence
<400> 5
gatgggcaga ggctactttg 20
<210> 6
<211> 20
<212> DNA
<213>Artificial sequence
<400> 6
gatgggcaga ggctactttg 20
<210> 7
<211> 20
<212> DNA
<213>Artificial sequence
<400> 7
gatgggcaga ggctactttg 20
<210> 8
<211> 20
<212> DNA
<213>Artificial sequence
<400> 8
gatgggcaga ggctactttg 20
Claims (7)
1. cotton fiber length correlation QTL qUHM-1-1, it is characterised in that the cotton fiber length correlation QTL qUHM-1-
1 is located at No. 1 chromosome, and its side neighbour's mark is.
2. cotton fiber length correlation QTL qUHM-22-1, it is characterised in that cotton fiber length correlation QTL qUHM-22-1
Positioned at No. 22 chromosomes, side neighbour's mark is.
3. cotton fiber length correlation QTL qUHM-1-1 specific SSR primers, it is characterised in that
The sequence of the primer is：
BNL3580F：5'CTTGTTTACATTCCCTTCTTTATACC 3'；
BNL3580R:5'CAAAGGCGAACTCTTCCAAA 3'；
BNL1667F:5'AGGTGCTTCAGGCATGATTC 3'；
BNL1667R:5'CCCTCACACCTAAACCCAAA 3'。
4. cotton fiber length correlation QTL qUHM-22-1 specific SSR primers, it is characterised in that
The sequence of the primer is：
NAU3093F：5'GATGGGCAGAGGCTACTTTG 3'
NAU3093R：5'GATGGGCAGAGGCTACTTTG 3'
CICR0345-F：5'GATGGGCAGAGGCTACTTTG 3'
CICR0345-R：5'GATGGGCAGAGGCTACTTTG 3'.
5. cotton fiber length correlation QTL qUHM-1-1 application described in claim 1.
6. cotton fiber length correlation QTL qUHM-22-1 application described in claim 2.
7. a kind of method for obtaining cotton fiber length correlation QTL, it is characterised in that the described method comprises the following steps：
(1) it is donor parents hybridization using upland cotton PD94042 as receptor parent, with yellowish-brown cotton (G.mustelinum), and with land
Ground cotton PD94042 is recurrent parent third backcross generation, then selfing three generations builds backcrossing advanced lines colony, selects covering cotton full genome
The introgressive line of group, carries out backcrossing advanced lines qtl analysis；
(2) introgressive line population data analysis includes：
(2-1) genotype data is counted
Record the type of parent and filial generation SSR marker；
(2) phenotypic data is counted
3 years fiber quality phenotypic datas are subjected to average value, scope, the coefficient of variation, degree of bias statistical analysis；
(3) genetic map is built to position with QTL
The genotype data of comprehensive introgressive line colony, with the corresponding sequence of each molecular labeling, compares Tetraploid G. hirsutum genome
Database, obtains the genomic locations of each molecular labeling, as reference information, the linkage relationship mark is analyzed,
The genetic linkage mapses of molecular labeling are built, then genotype data is combined with phenotypic data, carry out cotton fiber length QTL
Finely positioning.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111793714A (en) * | 2020-08-06 | 2020-10-20 | 南通大学 | Yellow-brown cotton fiber length related QTL and application thereof |
| CN112011638A (en) * | 2020-09-02 | 2020-12-01 | 南通大学 | Yellow-brown cotton fiber fineness related QTL and application thereof |
| CN114634993A (en) * | 2022-04-27 | 2022-06-17 | 南通大学 | Transcriptome and proteome combined analysis-based cotton salt-tolerant gene discovery method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613761A (en) * | 2009-08-12 | 2009-12-30 | 中国农业科学院棉花研究所 | The SSR mark chain with the cotton fiber strength major gene |
-
2017
- 2017-06-10 CN CN201710434758.XA patent/CN107201403B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613761A (en) * | 2009-08-12 | 2009-12-30 | 中国农业科学院棉花研究所 | The SSR mark chain with the cotton fiber strength major gene |
Non-Patent Citations (3)
| Title |
|---|
| HANTAO WANG等: "Identification of QTL for Fiber Quality and Yield Traits Using Two Immortalized Backcross Populations in Upland Cotton", 《PLOS ONE》 * |
| XINLIAN SHEN等: "Molecular mapping of QTLs for fiber qualities in three diverse lines in Upland cotton using SSR markers", 《MOLECULAR BREEDING》 * |
| 张科: "陆海杂交高代回交重组近交系纤维品质和产量分子标记研究", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111793714A (en) * | 2020-08-06 | 2020-10-20 | 南通大学 | Yellow-brown cotton fiber length related QTL and application thereof |
| CN112011638A (en) * | 2020-09-02 | 2020-12-01 | 南通大学 | Yellow-brown cotton fiber fineness related QTL and application thereof |
| CN114634993A (en) * | 2022-04-27 | 2022-06-17 | 南通大学 | Transcriptome and proteome combined analysis-based cotton salt-tolerant gene discovery method and application thereof |
| CN114634993B (en) * | 2022-04-27 | 2023-03-14 | 南通大学 | Transcriptome and proteome combined analysis-based cotton salt-tolerant gene discovery method and application thereof |
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