CN112011638A - Yellow-brown cotton fiber fineness related QTL and application thereof - Google Patents
Yellow-brown cotton fiber fineness related QTL and application thereof Download PDFInfo
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Abstract
The invention relates to the field of plant inheritance, in particular to a QTL (quantitative trait locus) related to the fineness of a yellow-brown cotton fiber and application thereof, and relates to the field of plant inheritance, in particular to the QTL related to the fineness of the yellow-brown cotton fiber and application thereof. Based on the QTL, the cotton material with prominent fiber fineness expression can be selected by molecular marker assistance. The QTL related to the fineness of the yellow-brown cotton fiber is detected in groups of three different generations, and has the stability independent of the environment.
Description
Technical Field
The invention relates to the field of plant inheritance, in particular to a QTL (quantitative trait locus) related to the fineness of a yellow-brown cotton fiber and application thereof.
Background
Cotton (Gossypium L.) comprises four cultivars, namely grass cotton (g. herbarum), asian cotton (g. arboreum), upland cotton (g. hirsutum) and sea island cotton (g. barbadense), which are the most important sources of textile fibers in the world, and the vast majority of cotton production comes from upland cotton cultivars. Through long-term human breeding improvement, the modern upland cotton cultivated species has a plurality of excellent characteristics suitable for human needs, such as high yield, high quality and the like; however, the selection of these superior traits in humans also results in a loss of cotton genetic diversity. Research shows that the modern upland cotton gene bank is very limited, and the development of upland cotton varieties faces serious bottleneck of genetic diversity. In the alloplastic cotton tetraploid, the rest are wild cotton except upland cotton and sea island cotton which are cultivars. The xanthobrown cotton as wild alloploid cotton is far away from upland cotton in genetic distance, so that some new gene loci are likely to exist, and the xanthobrown cotton can be used for genetic improvement of upland cotton. Research shows that the upland cotton strain with the introgression of the seed quality of the yellow-brown cotton has abundant genetic variation in the fiber quality character. Therefore, if the high-quality gene of the yellow-brown cotton can be introduced into the upland cotton, the improvement of the fiber quality of the upland cotton can be facilitated.
The genetic evaluation is planned to be carried out on the backcross high-generation population of the yellow-brown cotton and the upland cotton and the subsequent introduction line selected and constructed, and the excellent fiber quality gene of the yellow-brown cotton is explored.
Disclosure of Invention
Aiming at the problems, the invention provides the QTL related to the fineness of the fibers of the yellow-brown cotton and the application thereof, which are detected in groups of three different generations and have the stability independent of the environment.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the invention aims to provide a QTL qMIC-15-1 related to the fineness of the fibers of the fulvic cotton, wherein the QTL qMIC-15-1 related to the fineness of the fibers of the fulvic cotton is positioned on a No. 15 chromosome, and a molecular marker adjacent to the chromosome is BNL 1350.
The invention also aims to provide a specific SSR primer of the related QTL qMIC-15-1 of the fiber fineness of the cotton with the brown cotton, wherein the sequence of the primer is as follows:
| primer name | Primer sequence ((5'-3')) |
| BNL1350F | TAGGAGGAGAAGTTGGCGAA |
| BNL1350R | CAAGATGTGACCTTACCGCC |
The invention also aims to provide application of the QTL qMIC-15-1 related to the fineness of the fibers of the cotton with the fulvic acid.
The invention also provides a method for obtaining the QTL related to the fineness of the fibers of the yellow-brown cotton, which comprises the following steps:
(1) hybridizing with upland cotton PD94042 as acceptor parent and brown cotton (G.mustelinum) as donor parent, backcrossing for three generations with upland cotton PD94042 as recurrent parent, selfing to construct backcross high-generation population, and culturing at BC3F2:3And BC3F2:4Generation, backcross high generation QTL analysis is carried out; the backcross high-generation population data analysis comprises the following steps:
(1-1) identification of BC Using SSR molecular markers3F2The genotype of the population;
(1-2) pairs of BC3F2:3And BC3F2:4Carrying out phenotypic identification of fiber quality in generation;
(1-3) constructing a genetic map and positioning QTL;
synthesizing backcross high-generation population genotype data, comparing a tetraploid upland cotton genome database by using sequences corresponding to molecular markers to obtain the genome positions of the molecular markers, analyzing the linkage relationship among the markers by using the genome positions as reference information to construct a genetic linkage map of the molecular markers, and then combining the genotype data with phenotype data to carry out Quantitative Trait Locus (QTL) positioning on the fineness of the yellow-brown cotton fibers;
(2) selecting an introgression line covering the whole genome of cotton, and analyzing the data of the introgression line population by using the method comprising the following steps:
(2-1) identifying the genotype of the introgression line population by using SSR molecular markers;
(2-2) performing fiber quality phenotype identification on the introgression line population;
(2-3) constructing a genetic map and positioning QTL;
and (2) integrating the genotype data of the introgression line population, comparing a tetraploid upland cotton genome database by using the sequence corresponding to each molecular marker to obtain the genome position of each molecular marker, analyzing the linkage relation among the markers by using the genome position as reference information to construct a genetic linkage map of the molecular marker, and combining the genotype data with the phenotype data to develop the yellow-brown cotton fiber fineness QTL.
The invention has the beneficial effects that: the invention provides a yellow-brown cotton fiber fineness related QTL located on a cotton No. 15 chromosome, which is detected in three different generations and has environment-independent stability.
Drawings
FIG. 1 high-generation BC based on yellow-brown cotton backcross of the invention3F2Population-localized fiber fineness QTL (qMIC-15-1);
FIG. 2 shows a fiber fineness QTL (qMIC-15-1) based on the population localization of a brown and yellow cotton introgression line;
FIG. 3 shows that the near isogenic line is selected based on the fiber fineness QTL qMIC-15-1. (A represents homozygous upland cotton genotype, B represents yellow-brown cotton gene introgression)
Detailed Description
The technical solution of the present invention is explained below with reference to the drawings and examples.
Referring to fig. 1-3, the related QTL qMIC-15-1 of the fineness of the fulvic cotton fiber is located on chromosome 15, and the adjacent molecular marker is BNL 1350.
The invention also provides a specific SSR primer of the yellow-brown cotton fiber fineness related QTL qMIC-15-1, wherein the sequence of the primer is as follows:
| primer name | Primer sequence ((5'-3')) |
| BNL1350F | TAGGAGGAGAAGTTGGCGAA |
| BNL1350R | CAAGATGTGACCTTACCGCC |
The invention also provides application of the QTL qMIC-15-1 related to the fineness of the fibers of the fulvous cotton.
According to the specific embodiment of the invention, the cotton is upland PD94042 (P)1) Is the receptor parent and is made of brown cotton (G. mustelinum) (P)2) Crossing with upland cotton PD94042 (P)1) Backcross the third generation of parent recurrent, then construct backcross high generation group, breed a set of import line covering cotton whole genome from it, total 65 materials, carry on backcross high generation QTL analysis (AB-QTL). The analysis of the import group data comprises the following steps:
(1) genotype data statistics
Recording the types of parent and progeny SSR markers: with the parent (P)1) The same homozygous band pattern is designated "1"With the parent (P)2) The same homozygous band pattern was designated "2", the heterozygous band pattern of both parents was designated "3", the fuzzy or missing data was designated "0"; when the parent is (P)1) Being recessive, the parent (P)2) When dominant, P1The tape type is marked as "1", P2、F1The band pattern is marked as "4", when the parent is (P)1) Being dominant, the parent (P)2) When recessive, P2The tape type is denoted as "2", P1、F1The tape pattern is denoted as "5".
(2) Statistics of phenotypic data
BC3F2:3And BC3F2:4Statistical analysis of the fiber quality phenotype data of the generation and introduction population, such as mean, range, coefficient of variation, skewness, etc.
(3) Construction of genetic map and QTL location
And (3) synthesizing the genotype data of the population, comparing the sequence corresponding to each molecular marker with a tetraploid upland cotton genome database to obtain the genome position of each molecular marker, and analyzing the linkage relation among the markers by taking the genome position as reference information to construct the genetic linkage map of the molecular markers. And combining the genotype data and the phenotype data of the fiber quality, carrying out fiber quality QTL positioning and analyzing the result, detecting that the lowest LOD value of the QTL is 3.0, and finally, determining the position of the target QTL on a linkage map or a chromosome.
The QTL qMIC-15-1 related to the fineness of the fibers of the xanthochrous cotton is positioned on the No. 15 chromosome, the contribution rate is high, and the favorable genes (the fineness is reduced) of the QTL qMIC-15-1 related to the fineness of the fibers of the xanthochrous cotton are all from the xanthochrous cotton.
TABLE 1A QTL qMIC-15-1 for the fiber fineness of chromosome 15 from Phaseolus angularis
| QTL | Generation of generation | Nearest neighbor molecular marker | Contribution ratio (%) | Additive effect |
| qMIC-15-1 | BC3F2:3 | BNL1350 | 7.76 | 0.39 |
| BC3F2:4 | BNL1350 | 5.32 | 0.24 | |
| Population of introgression lines | BNL1350 | 32.68 | 0.53 |
Referring to fig. 3: based on the QTL, a set of near isogenic lines is selected in a fulvic cotton introduction line population in a molecular-assisted breeding mode, the excellent introduction lines including IL49 and IL53 are included, fulvic cotton introgression genes are arranged at the close-linked molecular marker BNL1350 and the vicinity thereof, and the rest parts are upland cotton genes. In conclusion, the 2 introduction lines all have the genes of the fulvic cotton around the major QTL of the fiber fineness, which shows that the genes are possibly greatly related to the beneficial genes of the fiber fineness. By integrating the introgression condition of the fulvic acid cotton gene on the Chr.15 chromosome, the introduction lines IL49 and IL53 have fulvic acid cotton genes related to fiber fineness on the Chr.15 chromosome and can be used as a preferential selection material for subsequent research.
Compared with the conventional QTL for stable fiber fineness, the QTL related to the fiber fineness of the xanthobrown cotton located on the chromosome 15 of the cotton is detected in three different generations and has the stability independent of the environment.
While one embodiment of the present invention has been described in detail, the description is only a preferred embodiment of the present invention and should not be taken as limiting the scope of the invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.
Claims (4)
1. The QTL qMIC-15-1 related to the fiber fineness of the fulvic-brown cotton is characterized in that the QTL qMIC-15-1 related to the fiber fineness of the fulvic-brown cotton is positioned on a No. 15 chromosome, and the adjacent molecular marker is BNL 1350.
2. The SSR primer for the related QTL qMIC-15-1 of the fiber fineness of the cotton with the brown and yellow color is characterized in that: the sequence of the primer is as follows:
BNL1350F：5'TAGGAGGAGAAGTTGGCGAA 3'；
BNL1350R：5'CAAGATGTGACCTTACCGCC 3'。
3. the application of the fulvous cotton fiber fineness related QTL qMIC-15-1 of claim 1.
4. A method for obtaining a QTL related to the fineness of a yellow-brown cotton fiber is characterized by comprising the following steps: the method comprises the following steps:
(1) hybridizing with upland cotton PD94042 as acceptor parent and brown cotton (G.mustelinum) as donor parent, backcrossing for three generations with upland cotton PD94042 as recurrent parent, selfing to construct backcross high-generation population, and culturing at BC3F2:3And BC3F2:4Generation, backcross high generation QTL analysis is carried out; the backcross high-generation population data analysis comprises the following steps:
(1-1) Using SSR molecular markersIdentification of BC3F2The genotype of the population;
(1-2) pairs of BC3F2:3And BC3F2:4Carrying out phenotypic identification of fiber quality in generation;
(1-3) constructing a genetic map and positioning QTL;
synthesizing backcross high-generation population genotype data, comparing a tetraploid upland cotton genome database by using sequences corresponding to molecular markers to obtain the genome positions of the molecular markers, analyzing the linkage relationship among the markers by using the genome positions as reference information to construct a genetic linkage map of the molecular markers, and then combining the genotype data with phenotype data to carry out Quantitative Trait Locus (QTL) positioning on the fineness of the yellow-brown cotton fibers;
(2) selecting an introgression line covering the whole genome of cotton, and analyzing the data of the introgression line population by using the method comprising the following steps:
(2-1) identifying the genotype of the introgression line population by using SSR molecular markers;
(2-2) performing fiber quality phenotype identification on the introgression line population;
(2-3) constructing a genetic map and positioning QTL;
and (2) integrating the genotype data of the introgression line population, comparing a tetraploid upland cotton genome database by using the sequence corresponding to each molecular marker to obtain the genome position of each molecular marker, analyzing the linkage relation among the markers by using the genome position as reference information to construct a genetic linkage map of the molecular marker, and combining the genotype data with the phenotype data to develop the yellow-brown cotton fiber fineness QTL.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107201403A (en) * | 2017-06-10 | 2017-09-26 | 南通大学 | Cotton fiber length correlation QTL and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107201403A (en) * | 2017-06-10 | 2017-09-26 | 南通大学 | Cotton fiber length correlation QTL and its application |
Non-Patent Citations (2)
| Title |
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| LIU，S等: ""Simple sequence repeat–based assessment of genetic diversity in cotton race stock accessions"" * |
| QI CHEN等: ""Validation of QTLs for Fiber Quality Introgressed from Gossypium mustelinum by Selective Genotyping"" * |
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