CN102511385A - Selection method of polymeric rice sheath blight resistance breeding material - Google Patents
Selection method of polymeric rice sheath blight resistance breeding material Download PDFInfo
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Abstract
The invention discloses a selection method of an anti-rice sheath blight breeding material. Varieties applied in production are taken as recurrent parents, local varieties or other cultivated varieties are taken as donor parents, and one time of hybridization, two times of backcross and two times of selfing are carried out to obtain a mixed group. Rice husk field dissemination method combined with toothpick embedding method is adopted and artificial inoculation is carried out for three consecutive generation to select a plant with an obviously lower degree of rice sheath blight disease than that of recurrent parents from the mixed group. Rice sheath blight site is detected by chi-square test, BC2F6 good disease resistant plant is selected to carry out hybridization, and a disease resistant polymeric line is screened by molecular maker selection and artificial inoculation. According to the invention, QTL location of target traits and variety improvement can be effectively integrated, genetic background effects of anti-sheath blight QTL can be overcome by polymerizing a disease resistant selected introgression line with a similar genetic background and disease resistant level of the rice line is improved.
Description
Technical field
The invention belongs to new crop varieties new material breeding technique field, be specifically related to a kind of selection of anti-rice sheath blight disease breeding material.
Background technology
The banded sclerotial blight that is caused by Rhizoctonia solani Kuhn (Rhizoctonia solani kuhn) is one of important fungal disease of paddy rice, and production loss is 10%-30%, can reach more than 50% when serious.A large amount of use chemical agents are prevented and treated disease and are not only caused environmental pollution easily, and cause expenses for prevention and control to rise year by year because the pesticide resistance of pathogen strengthens.Utilize antagonist to prevent and treat the deficiency that rice sheath blight disease can remedy chemical agent, therefore the further research but problems such as control period await cultivate and the plantation disease-resistant variety is prevented and treated one of most economical effective method of rice sheath blight disease.
Since the seventies in 20th century; The researcher carries out the sharp eyespot resistance evaluation to thousands of parts of rice germplasm resources both at home and abroad, finds that there is notable difference in rice varieties to the resistance of banded sclerotial blight, but does not find immunological species (Chen Zongxiang etc.; 2000; To the Primary Study in the anti-source of rice sheath blight disease, rice in China science, 14 (1): 15～18).The anti-source of the paddy rice that has identified is partial resistance mostly to sheath blight fungus, shows as the genetics of quantitative characters mode, receives a plurality of quantitative trait locus.Select to be in the traditional breeding method that phenotypic character according to plant carries out, very effective for the selection of monogenic inheritance, but this selection often produces little effect during in the face of the complex quantitative proterties.Therefore, compare with the serious rice disease of harm that pathogen such as bacterial blight of rice and host's disease-resistant gene exist typical specialization to do mutually, so far, paddy rice is not obtained effective progress to the resistance improvement of banded sclerotial blight always.
Development along with Protocols in Molecular Biology; Chinese scholars has been carried out the anti-banded sclerotial blight quantity of paddy rice site (Quantitative trait loci in succession; QTL) Position Research at present, all navigates to QTL (the Li ZK etc. of control banded sclerotial blight on 12 chromosomes of paddy rice; 1995; Characterization of quantitative trait loci (QTLs) in cultivated rice contributing to field resistance to sheath blight (Rhizoctonia solani), Theor Appl Genet, 91:374～381; Han Yuepeng etc., 2202, the QTL location of the bright extensive 63 pairs of banded sclerotial blight horizontal resistances of hybrid rice parent, Acta Genetica Sinica, 29 (7): 565～570).But, show that further the effect of the single site raising of the anti-banded sclerotial blight of paddy rice resistance is less, and have significant background dependence; Even under identical environmental condition; The most anti-banded sclerotial blight QTL that in a rice genetic background, expresses does not often express (Xie Xuewen etc., 2008, genetic background and environmental effect that the anti-banded sclerotial blight QTL of paddy rice expresses in another genetic background; Acta Agronomica Sinica; 34 (11): 1885～1893), therefore, though the researcher has identified some QTL from different disease-resistant varieties; But because most of genes/QTL location practice common and genetic breeding in the past disconnects fully, the molecular marker assisted selection technology is difficult to improve effectively the resistance of rice varieties to banded sclerotial blight at present; In addition, the conventional strategy that the resistant strain system through different genetic backgrounds hybridizes also is difficult to obtain disease resistance further improves anti-banded sclerotial blight polymerization system.
In recent years; Find through backcross population being carried out the objective trait screening; The entrained various beneficial genes of donor parents are to exist with hidden form mostly; The back cross breeding technology is to discover and use effective ways (Lafitte H R etc., 2006, the Improvement of rice drought tolerance through backcross breeding:evaluation of donors and results from drought nurseries that crytogene in the paddy rice original gene storehouse is improved complex characters such as drought resistance, cold resistance; Field Crop Res, 97 (1): 77-86).Screening to backcross progeny colony objective trait needn't stick to this performance on proterties of donor parents, and backcross population is through the strain system that all can obtain the objective trait improvement with Phenotypic Selection that coerces of strictness.
Summary of the invention
The purpose of this invention is to provide a kind of through making up the method that the disease-resistant selection introgressive line close with polymerization genetic background improves resisting rice sheath blight.
The object of the invention is realized through following technical scheme:
(1) BC
2F
2The structure of introgressive line colony and the screening of anti-banded sclerotial blight introgressive line.
To produce rice varieties or the hybrid rice parents used is recurrent parent, is donor parents with landrace or other rice varieties, hybridizes 1 time, backcrosses 2 times, in backcross process, selects 20～25 individual plants and recurrent parent to backcross at random, up to obtaining BC
2, all F that backcross then
1A family selfing generation will be from the BC of each combination
2F
2The selfed seed of individual plant mixes to be received, and forms the mixing group of each hybrid combination height for backcross population, so that there is abundant backcross progeny colony seed to carry out anti-banded sclerotial blight character test and screening.BC from each combination
2F
2Mix in the group and get 300～500 seeds, adopt the field to shed the rice husk inoculation method in the Sheng phase of tillering and carry out the banded sclerotial blight inoculation.After the inoculation, wait to ear and carry out the state of an illness investigation when left and right sides scab was stablized in back 30 days, select 20-40 occurring degree significantly to be lower than the strain system of recurrent parent.
It is paddy and rice husk to be packed into after by 3: 1 mixed sterilize in the triangular flask that rice husk inoculation method concrete grammar is shed in the field; Inoculating pathogen then cultivated 15 to 20 days; Treat mycelium to be covered with bottle base portion and bottle top when having sclerotium to grow; Carry out the secondary enlarged culture again, after 3 days mixture is spread fertilizer over the fields between paddy base portion or paddy rice row system.
(2) tracking of introgressive line offspring sharp eyespot resistance is identified.
With 20～40 BC that obtain
2F
3Resistant strain is each strain system kind of two row, every row 10 strains, and distance between rows and hills is 20cm * 15cm, 2 repetitions are adopted every interline 6 strains of toothpick embedding inlay technique artificial infection in the Sheng phase of tillering, and carry out sharp eyespot resistance and identify, results BC
2F
4Disease-resistant strain is.
Toothpick embedding inlay technique concrete grammar is that the wooden stub of 10 * 1.5 * 1mm is tiled in the culture dish bottom; Pour potato dextrose agar then into; Sterilized 15 minutes for 121 ℃; The mycelium inoculation that will be grown on the solid culture medium with card punch is then cultivated in the culture dish of sterilizing, treat mycelia intensive cover with medium the time, just can carry out field inoculation.With tweezers stub is embedded three leaf sheaths inboard, and guarantee that leaf sheath phimosis state is unaffected, make plant be the natural occurrence state.
After the inoculation, wait to ear and carry out the state of an illness investigation when left and right sides scab was stablized in back 30 days.Calculate relative scab height tolerance, the ratio of promptly absolute scab height and sword-like leave pulvinus height.
(3) genotyping and resistance QTL location.
Select polymorphic SSR mark is arranged to BC between evenly distribution and parent from 12 chromosomes of paddy rice
2F
4Backcross population carries out genotyping.Collection of illustrative plates with reference to Cornell university confirms respectively to be marked at position and the genetic distance on the chromosome.Carry out genotyping with SSR mark antagonism banded sclerotial blight introgressive line; According to the heredity theory of getting a lift; To the genotyping result side's of card detection; The donor gene frequency will be counted as the site relevant with sharp eyespot resistance with the expected frequency site that there were significant differences, with the threshold value of P＜0.005 as the anti-banded sclerotial blight QTL of discovery.
(4) disease-resistant introgressive line agronomic shape investigation and disease-resistant polymerization are that colony makes up.
With the BC that is obtained
2F
4In generation, select introgressive line to plant in the area that is suitable for the recurrent parent growth, adopts the completely random block design, and single this is inserted plantation; Planting density is 20cm * 15cm; 10 strains, 1 row, each strain system plantation repeats for 4 times, and wherein banded sclerotial blight inoculation and non-inoculation test are provided with 2 repetitions respectively.Shallow irrigation, the fertilising and the extermination of disease and insect pest are as general land for growing field crops.
Contain the phase at plant tillering, adopt the toothpick embedding inlay technique every interline 6 strains inoculation sheath blight fungus.Investigate each 6 middle strain of strain system, put down in writing heading stage.Carry out the measurement of scab height, sword-like leave pillow height and plant height after the banded sclerotial blight stable disease in the field.Three strains of from middle 6 strains, selecting the growing way uniformity when ripe and not lacking on every side are used to investigate the output correlated traits; Comprise the effective fringe of individual plant, spike length, the real grain of every fringe number, every total grain panicle number, ripening rate and thousand kernel weight, These parameters is measured single plant yield with three strain mixing threshings after investigating and finishing.
Choosing good disease-resistant strain respectively from each combination is, the polymerization of disease-resistant QTL is carried out in configuration hybridization in twos in the close genetic background.F
1Through the true and false hybrid of molecular markers for identification, adopt toothpick embedding inlay technique inoculation F
2Polymerization colony.
Figure of description
Fig. 1 F
2The frequency distribution of the relative scab height of the banded sclerotial blight of polymerization colony
The distribution of Qsb6b (RM469) and Qsb1a (RM86) in Fig. 2 BSB1/BSB2 colony
RP, P1 and P2 represent bright extensive 86, polymerization parent BSB1 of recurrent parent and BSB2 respectively, and wherein the recurrent parent banding pattern is A, and different donor parents banding patterns is B, and the mixing banding pattern is H.
*Be expressed as the homozygous lines that contains Qsb6b and two sites of Qsb1a.
Embodiment
Below in conjunction with the practical implementation instance, further set forth the present invention.Should be appreciated that following instance only to be used to the present invention is described and be not used in and limit requirement protection domain of the present invention.Be recurrent parent with bright extensive 63 in the application example, closing hemp paddy the Meng and Jiangxi silk seedling is a donor parents, but the parent that the actual the present invention of being applicable to of the present invention adopts and other rice varieties are the colony that the parent constitutes.
(1) screening of the good selection introgressive line of anti-banded sclerotial blight
With the hybrid rice restoring line of producing extensive use bright extensive 86 is recurrent parent, and closing hemp paddy the Meng with rice varieties Jiangxi silk seedling and landrace is donor parents, prepares BC respectively
2F
2Population mixture.Spring in 2005 is each 400 BC of two colonies of plantation in breeding base, Chinese Academy of Agricultural Sciences Changping
2F
2Individual plant in rice tillering latter stage, adopts and sheds the rice husk inoculation method and carry out resistance and identify that the sheath blight fungus bacterial strain is the strong pathogenic strain RH-9 of Jiangsu Dao Qu.From two colonies, respectively screen the individual plant that 24 occurring degrees are lower than recurrent parent.Winter in 2005 was come self-evident extensive 86/ Jiangxi silk seedling and 24 bright extensive BC that close hemp paddy colony 86/ Meng with 24
2F
3Strain system is planted in the field piece of Hainan Island isolation, and 2 repetitions are set respectively, and every row 10 strains are adopted the inoculation of toothpick embedding inlay technique in late tillering state, select disease-resistant strain to be, obtain BC
2F
4Seed; Spring in 2006 in breeding base, Chinese Academy of Agricultural Sciences Changping to BC
2F
4Strain system adopts the inoculation of toothpick embedding inlay technique to identify that observing strain is the stability of disease resistance once more.
Spring in 2007 is with the BC that screens
2F
5Strain system is planted in Agricultural University Of Anhui experiment base, adopts single this of completely random block design to insert plantation, and each strain system plantation repeats for 4 times, and wherein banded sclerotial blight inoculation and non-inoculation test are provided with 2 repetitions respectively.Adopt the toothpick embedding inlay technique in plant tillering Sheng phase inoculation sheath blight fungus, investigate each 6 middle strain of strain system, put down in writing heading stage.Carry out the measurement of scab height, sword-like leave pillow height and plant height after the banded sclerotial blight stable disease in the field.From middle 6 strains, select three strains of growing way uniformity to be used to investigate the output correlated traits when ripe; Comprise the effective fringe of individual plant, spike length, the real grain of every fringe number, every total grain panicle number, ripening rate and thousand kernel weight, These parameters is measured single plant yield with three strain mixing threshings after investigating and finishing.
Bright extensive 86 average scab height relatively are 0.63, and Jiangxi silk seedling is respectively 0.46 and 0.47 with the average relative scab height that closes hemp paddy the Meng.For the ease of to selecting validity to describe, in inoculation and following sharp eyespot resistance of comparing of normal growth condition and fractional yield performance, introgressive line is divided into 4 types with recurrent parent according to introgressive line.
On behalf of sharp eyespot resistance, the T1 type be significantly increased than recurrent parent, under two kinds of wherein a kind of conditions of conditioned disjunction of artificial infection sheath blight fungus and normal plantation, is significantly increased with recurrent parent comparison output.Have 2 strain systems to belong to this type in 2 colonies, average scab aspect ratio relatively bright extensive 86 reduces by 0.20 under the artificial infection sheath blight fungus condition, and output increases 4.3t/ha, and average yield increases 2.7t/ha than bright extensive 86 under the normal planting conditions.
Table 1 is that the Other Main Agronomic Characters of 2 introgressive line colonies of recurrent parent is with respect to bright extensive 86 performances under two kinds of conditions of artificial infection sheath blight fungus and normal plantation with bright extensive 86
S represents the artificial infection sheath blight fungus, and N represents the normal growth condition, and JXSM represents Jiangxi silk seedling, and MGDMG represents and closes hemp paddy the Meng, and Minghui86 represents bright extensive 86.
*,
*With
* *Represent P＜0.05 in the t test respectively, the significance level of P＜0.01 and P＜0.001.
On behalf of sharp eyespot resistance, the T2 type be significantly increased than recurrent parent, but under two kinds of conditions of artificial infection sheath blight fungus and normal plantation, does not have marked difference with recurrent parent comparison output.Have 9 strain systems to belong to this type in 2 colonies, average scab aspect ratio relatively bright extensive 86 reduces by 0.10 under the artificial infection sheath blight fungus condition.
On behalf of sharp eyespot resistance and recurrent parent, the T3 type do not have marked difference, but output is significantly higher than recurrent parent under two kinds of wherein a kind of conditions of conditioned disjunction of artificial infection sheath blight fungus and normal plantation.Have 7 strain systems to belong to this type in 2 colonies, average yield increases 2.5t/ha and 1.5t/ha than bright extensive 86 respectively under two kinds of conditions of artificial infection sheath blight fungus and normal plantation.
On behalf of sharp eyespot resistance and recurrent parent, the T4 type do not have marked difference, and output is compared with recurrent parent under artificial infection sheath blight fungus and two kinds of conditions of normal plantation and all do not had marked difference.There are 29 strain systems to belong to this type in 2 colonies.
Or output significantly is lower than recurrent parent under wherein a kind of condition, and such strain is that proportion is very little.There is 1 strain system to belong to this type in 2 colonies.
Select polymorphic SSR mark is arranged to BC between evenly distribution and parent from 12 chromosomes of paddy rice
2F
4Backcross population carries out genotyping.Collection of illustrative plates with reference to Cornell university confirms respectively to be marked at position and the genetic distance on the chromosome.Carry out genotyping with SSR mark antagonism banded sclerotial blight introgressive line, based on the heredity theory of getting a lift, to the genotyping result side's of card detection, with the threshold value of P＜0.005 as the anti-banded sclerotial blight QTL of discovery.In 533 SSR marks, bright extensive 86 with Jiangxi silk seedling, close the Meng in the combination of hemp paddy and have 60 and 52 marks to present polymorphism preferably respectively, adopt these marks that introgressive line colony is carried out genotyping.
BC
2F
2Theory expectation for random population heterozygous genes type and donor parents homozygous genotype frequency is respectively 0.125 and 0.0625, and the theory expectation that donor gene imports frequency is 0.125.Select introgressive line colony donor parents homozygous genotype frequency averaging value to be respectively 0.13 and 0.17 for 6 in this research, the mean value of heterozygous genes type frequency is respectively 0.05 and 0.04, has obviously departed from theory expectation.The mean value of donor gene importing frequency is respectively 0.15 and 0.19 and has departed from theory expectation equally, shows that the selection effect is obvious, meets the characteristic of selecting colony.
It is 0.005 that detection threshold is set, and detects selecting colony's marker gene type frequency to carry out card side.2 colonies that select for use detect altogether selects the back to import 15 in the site of frequency marked change, and according to the heredity theory of getting a lift, these sites possibly be exactly the QTL site relevant with resisting rice sheath blight.Minghui86/JXSM colony detects 8, is respectively Qsb1a, Qsb1b, Qsb2a, Qsb2b, Qsb3a, Qsb6a, Qsb7a and Qsb8; Close hemp paddy colony and detect 7 bright extensive 86/ Meng, is respectively Qsb1c, Qsb2c, Qsb6c, Qsb6a, Qsb7c, Qsb7b and Qsb10.
5 QTLs in the bright extensive 86/ Jiangxi silk seedling colony, i.e. Qsb1a, Qsb1b, Qsb2a, Qsb3a and Qsb7a have showed the negative sense additive effect to relative scab altimeter, and all the other QTLs all show the forward additive effect to relative scab height; Close 3 QTLs in the hemp colony bright extensive 86/ Meng, promptly Qsb2c, Qsb7c and Qsb10 have showed the negative sense additive effect to relative scab altimeter, and all the other QTLs all show the forward additive effect (table 2) to relative scab height.
Table 2 closes detected anti-banded sclerotial blight QTL in the hemp paddy introgressive line Meng at paddy rice Minghui86/TQ and Minghui86/
(2) anti-banded sclerotial blight is selected the polymerization of introgressive line
Spring in 2009,, select self-evident extensive 86/ Jiangxi silk seedling and the bright extensive BC that closes hemp paddy colony 86/ Meng according to the result of (1)
2F
6Strain is that BSB1 and BSB2 are hybridized as male parent and female parent respectively, and BSB1 then carries Qsb1a, Qsb3a and three resistance sites of Qsb8c; BSB2 carries three resistance sites, is respectively Qsb2c, Qsb6b and Qsb7b, analyzes disease-resistant introgressive line QTL assembly effect.
Table 3 polymerization is parent's a economical character
With the linked marker in 4 sites to F
1Hybrid is identified, selects real crossbreed results.Passed through the toothpick embedding inlay technique in winter in 2009 to F in Hainan
2Polymerization colony carries out disease-resistant evaluation, and susceptible contrast Lemont is relative, and the scab height reaches 0.81, and the relative scab height of recurrent parent bright extensive 86 is 0.67, and the relative scab height of BSB1 and BSB2 is 0.47 and 0.49.
The F of polymerization colony
2Distribution such as Fig. 1 of relative scab height frequency, wherein will to surpass the resistance strain similar with recurrent parent significantly be to have shown after the polymerization to have tangible assembly effect to the strain coefficient that significantly increases of resistance.In the colony relatively the scab height be respectively 16 strains less than 0.3 strain system, account for F
27.5% of colony's sum.
To carry out combinatory analysis from the resistance site of different donors and find, Qsb6b, Qsb7b and have evident difference from the assembly effect between 3 resistance sites of parent BSB1.The assembly effect of itself and Qsb1a is best, with the assembly effect of Qsb3a secondly, the poorest with the assembly effect of Qsb8c.Qsb3a+Qsb6b, sick level is remarkable with the sick level difference in single site after the Qsb3a+Qsb7b polymerization, but and Qsb8c+Qsb6b, sick level difference is not remarkable during the Qsb8c+Qsb7b polymerization.Qsb1a+Qsb6b, the sick level of Qsb1a+Qsb7b polymerization significantly is lower than the sick level of other four polymerizations, explains that the assembly effect that Qsb1a and Qsb6b or Qsb7b condense together is the most obvious.Fig. 2 be the chain mark of employing and Qsb1a and Qsb6b to the partial results that colony analyzes, detecting the strain of all isozygotying in 2 sites is 13.
Table 4 has been listed two Qsb1a+Qsb6b polymerization systems and parent's thereof relative scab height and Other Main Agronomic Characters, and the result shows and adopts the close sharp eyespot resistance of genetic background to select introgressive line can obtain the rice strain that resistance significantly improves, single plant yield is superior to recurrent parent.Antagonism characteristic of disease and economical character all significantly are superior to the polymerization system of recurrent parent, further carry out disease resistance and economical character evaluation and Analysis of Combining Ability in the strain system, can obtain the stronger hybrid rice restoring line of disease resistance.
The economical character of table 4 polymerization system
The present invention compared with prior art has the following advantages and effect:
(1) existing most QTL target groups and breeding population are independent fully; Because the expression in resisting rice sheath blight site is influenced by genetic background significantly; The result of QTL location is difficult to directly be used for molecular mark, and the present invention can carry out organic combination with the QTL location of objective trait with breed improvement effectively.
(2) paddy rice is a quantitative character to the resistance of banded sclerotial blight, and the single site of transformation is difficult to improve effectively the sharp eyespot resistance of paddy rice, and different Q TL polymerization is owing to receive the influence of genetic background often not reach the effect that improves resistance.The present invention can overcome the influence that genetic background is expressed QTL through the close disease-resistant selection introgressive line of polymerization genetic background, realizes effective improvement of objective trait through adding up of a plurality of beneficial genes.
(3) the present invention adopts the cultivar of using in the production to make recurrent parent, from BC
2F
2The disease-resistant strain that population mixture screening acquisition has excellent agronomic characters is to hybridize, and is beneficial to and cultivates the rice varieties that using value is arranged.
Claims (2)
1. the selection of an anti-banded sclerotial blight rice breed.It is characterized in that to produce the kind recurrent parent of application; With landrace or other cultivar is donor parents; Hybridize 2 acquisitions of the selfing population mixture again of backcrossing for 1 time 2 times; Adopt the field to shed the individual plant that rice husk method screening banded sclerotial blight occurring degree is lower than recurrent parent, adopt two generations of toothpick embedding inlay technique continuous inoculation again.Utilize the anti-banded sclerotial blight of card side's detection and location site, select the BC that resistance is stable, genetic background is close
2F
6Elite plant strain is hybridized, through molecular labeling select and artificial infection from F
2The disease-resistant polymerization of screening system in the colony.
2. the selection of anti-banded sclerotial blight rice breed according to claim 1 is characterized in that the method for utilizing the close anti-banded sclerotial blight of genetic background to select introgressive line to carry out the resistance polymerization related in the said method.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102754591A (en) * | 2012-07-03 | 2012-10-31 | 福建农林大学 | Breeding method of multi-resistance rice germplasm |
| CN105475120A (en) * | 2015-12-11 | 2016-04-13 | 广东省农业科学院水稻研究所 | Molecular breeding method for improving cold resistance of rice |
| CN106577256A (en) * | 2016-12-03 | 2017-04-26 | 安徽省农业科学院水稻研究所 | Breeding method for rapidly introducing rice target characters |
-
2011
- 2011-12-21 CN CN2011104318471A patent/CN102511385A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 高晓清等: "水稻抗纹枯病导入系的构建及抗病位点的初步定位", 《作物学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102754591A (en) * | 2012-07-03 | 2012-10-31 | 福建农林大学 | Breeding method of multi-resistance rice germplasm |
| CN102754591B (en) * | 2012-07-03 | 2013-10-23 | 福建农林大学 | Breeding method of multi-resistance rice germplasm |
| CN105475120A (en) * | 2015-12-11 | 2016-04-13 | 广东省农业科学院水稻研究所 | Molecular breeding method for improving cold resistance of rice |
| CN106577256A (en) * | 2016-12-03 | 2017-04-26 | 安徽省农业科学院水稻研究所 | Breeding method for rapidly introducing rice target characters |
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Application publication date: 20120627 |