CN106577252A - Dendrobium officinale pollination method - Google Patents
Dendrobium officinale pollination method Download PDFInfo
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- CN106577252A CN106577252A CN201610989614.6A CN201610989614A CN106577252A CN 106577252 A CN106577252 A CN 106577252A CN 201610989614 A CN201610989614 A CN 201610989614A CN 106577252 A CN106577252 A CN 106577252A
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- pollen
- herba dendrobii
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- pollination
- pollinating
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention discloses a dendrobium officinale pollination method. The method comprises the steps of 1 pollen collection, 2 pollen carrier solution preparation, 3 pollen turbid liquid preparation, 4 pollination and 5 management performed after pollination. By preparing the pollen carrier solution, pollen grains and the pollen carrier solution are subjected to ultrasonic homogeneity to obtain the dendrobium officinale pollen granulosity turbid liquid, then, a dropper is used for performing pollination on dendrobium officinale through the dendrobium officinale pollen granulosity turbid liquid, the phenomenon that maternal stigmas are injured through a traditional artificial pollination method is avoided, the pollination success rate and the fruit setting rate are greatly increased, the pollination success rate reaches 99.8% or above, and the fruit setting rate reaches 99.5 or above.
Description
Technical field
The present invention relates to Herba Dendrobii pollination field, more particularly to a kind of Herba Dendrobii pollinating method.
Background technology
Herba Dendrobii is orchid family Dendrobium herbaceos perennial, is the famous and precious rare Chinese crude drug of China's tradition, with nourishing the stomach
Promote the production of body fluid, nourishing YIN and clearing away heat, improve body immunity the effects such as.At present, Herba Dendrobii be listed in special-protection-by-the-State medicinal plants,
The class protection plant of the world two.
Herba Dendrobii flower causes the fruiting rate of Herba Dendrobii under natural conditions extremely low because of its special physiological structure, Herba Dendrobii one
As May be full-bloom stage, florescence 10-15d, its fruit be capsule, easily split when ripe, seed ballooning is scattered, and these are thin
, containing only immature embryo, without endosperm, natural propagation is very difficult for the kind Intradermal of little seed.With modern plants tissue culture technology
Development, people are carried out the sprouting of Herba Dendrobii embryo by tissue culture technology and breed become quite ripe.Herba Dendrobii embryo is obtained
One of factor of Herba Dendrobii industrialized development must be had become, the acquisition of current Herba Dendrobii embryo is mainly pollinated by Traditional Man
And obtain, there is Traditional Man pollinating method operating technology to have high demands, place property requires harshness, high labor intensive, production efficiency
The shortcomings of low and empty fruit rate of low, fruit-setting rate is high.
The content of the invention
It is an object of the invention to provide a kind of Herba Dendrobii pollinating method, is achieved through the following technical solutions：
A kind of Herba Dendrobii pollinating method, comprises the following steps：
(1) collection of pollen：Collect pollen grain of the florescence in the Herba Dendrobii flower of 2-3 days；
(2) preparation of pollen carrier fluid：By weight 2 parts of TIEGUANYIN tea and boiling water are pressed into 1:The sealing immersion of 80 mass ratios
After 10min, filter, the TIEGUANYIN tea after obtaining filtrate A and soaking, by filtrate A, cold preservation is stand-by at 2-4 DEG C, after immersion
Water of the TIEGUANYIN tea with 80-85 DEG C presses 1:20 mass ratio sealing thermal insulations soak 2 days, then filter and remove TIEGUANYIN tea, obtain
Liquor B, the sodium carboxymethyl cellulose of TIEGUANYIN quality 15.5% is added in liquor B, is stirred, and TIEGUANYIN is then added again
The glycerol that 2 times of quality, after stirring, obtains final product required pollen carrier fluid；The pollen carrier fluid that the present invention is prepared can be more preferable
Pollen grain is carried, and pollination success rate can greatly be improved；
(3) preparation of pollen suspension：The pollen grain that step (1) is collected is added in the pollen carrier fluid that step (2) is produced
Afterwards, homogenize Herba Dendrobii pollen grain group suspension using ultrasound；
(4) pollinate：Herba Dendrobii pollen grain is rolled into a ball into suspension using glue head dropper to award the Herba Dendrobii flower bloomed 3 days
Powder, every Herba Dendrobii chapiter instills 2-3 Herba Dendrobii pollen grain group；
(5) manage after pollinating：After pollination 10 days, the sucrose of its quality 1.5% is added in filtrate A, stirring obtains solution after melting
C, solution C presses 1:50 mass ratios are watered, and spray the Herba Dendrobii after pollinating 10 days.
Further, preserve 2 hours at being placed on 6-8 DEG C after the collection of the pollen grain of the step (1).
Further, preserve 4 hours at the pollen carrier fluid of the step (2) is placed on 6-8 DEG C after preparing.
Further, ultrasonic frequency is 22KHz in ultrasonic homogenizing in the step (3), and ultrasonic power is 250W, is surpassed
The sonication time is 20 seconds, on the basis of 1.5L materials.
Further, after solution C is watered in the step (5), fountain height is 5kg/ mus.
Beneficial effect of the present invention：The present invention passes through to prepare pollen carrier fluid, by pollen grain and pollen carrier fluid ultrasound homogenizing
After obtain Herba Dendrobii pollen grain group suspension, then using glue head dropper by Herba Dendrobii pollen grain roll into a ball suspension to iron sheet stone
Dry measure used in former times flower is pollinated, and not only avoid phenomenon of the Traditional Man pollinating method to the damage of maternal stigma, is also greatly improved
Pollination success rate and fruit-setting rate are high, and pollination success rate reaches more than 99.8%, and fruit-setting rate reaches more than 99.5, and without empty fruit
Phenomenon occur, the Herba Dendrobii capsule of maturation is obtained bearing fruit 105 days or so by, fruit is uniformly full, greatly shortens results
Phase, the process of 10 days after pollination can not only be further ensured that the phenomenon without empty fruit occurs, moreover it is possible to greatly improve Herba Dendrobii
The yield and quality of capsule.
Specific embodiment
Embodiment 1
A kind of Herba Dendrobii pollinating method, comprises the following steps：
(1) collection of pollen：Collect pollen grain of the florescence in the Herba Dendrobii flower of 2 days；
(2) preparation of pollen carrier fluid：By weight 2 parts of TIEGUANYIN tea and boiling water are pressed into 1:The sealing immersion of 80 mass ratios
After 10min, filter, the TIEGUANYIN tea after obtaining filtrate A and soaking, by filtrate A, cold preservation is stand-by at 2 DEG C, by the ferrum after immersion
Kwan-yin tea and 80 DEG C of water press 1:20 mass ratio sealing thermal insulations soak 2 days, then filter and remove TIEGUANYIN tea, obtain liquor B,
Add the sodium carboxymethyl cellulose of TIEGUANYIN quality 15.5% in liquor B, stir, 2 times of TIEGUANYIN quality is then added again
Glycerol, after stirring, obtain final product required pollen carrier fluid；
(3) preparation of pollen suspension：The pollen grain that step (1) is collected is added in the pollen carrier fluid that step (2) is produced
Afterwards, homogenize Herba Dendrobii pollen grain group suspension using ultrasound；
(4) pollinate：Herba Dendrobii pollen grain is rolled into a ball into suspension using glue head dropper to award the Herba Dendrobii flower bloomed 3 days
Powder, every Herba Dendrobii chapiter instills 2- Herba Dendrobii pollen grain group；
(5) manage after pollinating：After pollination 10 days, the sucrose of its quality 1.5% is added in filtrate A, stirring obtains solution after melting
C, solution C presses 1:50 mass ratios are watered, and spray the Herba Dendrobii after pollinating 10 days.
Further, preserve 2 hours at being placed on 6 DEG C after the collection of the pollen grain of the step (1).
Further, preserve 4 hours at the pollen carrier fluid of the step (2) is placed on 6 DEG C after preparing.
Further, ultrasonic frequency is 22KHz in ultrasonic homogenizing in the step (3), and ultrasonic power is 250W, is surpassed
The sonication time is 20 seconds, on the basis of 1.5L materials.
Further, after solution C is watered in the step (5), fountain height is 5kg/ mus.
Embodiment 2
A kind of Herba Dendrobii pollinating method, comprises the following steps：
(1) collection of pollen：Collect pollen grain of the florescence in the Herba Dendrobii flower of 3 days；
(2) preparation of pollen carrier fluid：By weight 2 parts of TIEGUANYIN tea and boiling water are pressed into 1:The sealing immersion of 80 mass ratios
After 10min, filter, the TIEGUANYIN tea after obtaining filtrate A and soaking, by filtrate A, cold preservation is stand-by at 4 DEG C, by the ferrum after immersion
Kwan-yin tea and 85 DEG C of water press 1:20 mass ratio sealing thermal insulations soak 2 days, then filter and remove TIEGUANYIN tea, obtain liquor B,
Add the sodium carboxymethyl cellulose of TIEGUANYIN quality 15.5% in liquor B, stir, 2 times of TIEGUANYIN quality is then added again
Glycerol, after stirring, obtain final product required pollen carrier fluid；
(3) preparation of pollen suspension：The pollen grain that step (1) is collected is added in the pollen carrier fluid that step (2) is produced
Afterwards, homogenize Herba Dendrobii pollen grain group suspension using ultrasound；
(4) pollinate：Herba Dendrobii pollen grain is rolled into a ball into suspension using glue head dropper to award the Herba Dendrobii flower bloomed 3 days
Powder, every Herba Dendrobii chapiter instills 3 Herba Dendrobii pollen grain groups；
(5) manage after pollinating：After pollination 10 days, the sucrose of its quality 1.5% is added in filtrate A, stirring obtains solution after melting
C, solution C presses 1:50 mass ratios are watered, and spray the Herba Dendrobii after pollinating 10 days.
Further, preserve 2 hours at being placed on 8 DEG C after the collection of the pollen grain of the step (1).
Further, preserve 4 hours at the pollen carrier fluid of the step (2) is placed on 8 DEG C after preparing.
Further, ultrasonic frequency is 22KHz in ultrasonic homogenizing in the step (3), and ultrasonic power is 250W, is surpassed
The sonication time is 20 seconds, on the basis of 1.5L materials.
Further, after solution C is watered in the step (5), fountain height is 5kg/ mus.
Comparative example 1：It is only by the preparation of pollen carrier fluid with the difference of embodiment 1：By weight by 2 parts of TIEGUANYIN tea with
Boiling water presses 1:After 80 mass ratios sealing immersion 10min, filter, the TIEGUANYIN tea after obtaining filtrate A and soaking, by filtrate A mono-
Partly cold preservation is stand-by at 2 DEG C, and the sodium carboxymethyl cellulose of TIEGUANYIN quality 15.5% is added in second half filtrate A, and stirring is equal
It is even, the glycerol of 2 times of TIEGUANYIN quality is then added again, after stirring, obtain final product required pollen carrier fluid.
Comparative example 2：The clear water with the equivalent of embodiment 1 is sprayed after 10 days with the difference of embodiment 1 only pollination.
Pollination success rate compares with fruit-setting rate
Table 1
| Pollination success rate % | Fruit-setting rate % | |
| Embodiment 1 | 99.9 | 99.8 |
| Embodiment 2 | 99.8 | 99.7 |
| Comparative example 1 | 98.9 | 98.5 |
| Comparative example 2 | 99.4 | 99.1 |
As can be seen from Table 1, pollinating method pollination success rate of the present invention and fruit-setting rate are all very high.
Herba Dendrobii flower to blooming different number of days is pollinated, contrast pollination success rate（Except to Herba Dendrobii flower in full bloom
Natural law when being pollinated is different, and other are same as Example 1)：
Table 2
| 1 day | 2 days | 3 days | 4 days | 5 days | |
| Pollination success rate % | 96.4 | 97.9 | 99.9 | 98.2 | 96.1 |
As can be seen from Table 2, to being pollinated using the inventive method in Herba Dendrobii flower in full bloom 3 days, pollination success rate is most
It is high.
Claims (5)
1. a kind of Herba Dendrobii pollinating method, it is characterised in that comprise the following steps：
(1) collection of pollen：Collect pollen grain of the florescence in the Herba Dendrobii flower of 2-3 days；
(2) preparation of pollen carrier fluid：By weight 2 parts of TIEGUANYIN tea and boiling water are pressed into 1:The sealing immersion of 80 mass ratios
After 10min, filter, the TIEGUANYIN tea after obtaining filtrate A and soaking, by filtrate A, cold preservation is stand-by at 2-4 DEG C, after immersion
Water of the TIEGUANYIN tea with 80-85 DEG C presses 1:20 mass ratio sealing thermal insulations soak 2 days, then filter and remove TIEGUANYIN tea, obtain
Liquor B, the sodium carboxymethyl cellulose of TIEGUANYIN quality 15.5% is added in liquor B, is stirred, and TIEGUANYIN is then added again
The glycerol that 2 times of quality, after stirring, obtains final product required pollen carrier fluid；
(3) preparation of pollen suspension：The pollen grain that step (1) is collected is added in the pollen carrier fluid that step (2) is produced
Afterwards, homogenize Herba Dendrobii pollen grain group suspension using ultrasound；
(4) pollinate：Herba Dendrobii pollen grain is rolled into a ball into suspension using glue head dropper to award the Herba Dendrobii flower bloomed 3 days
Powder, every Herba Dendrobii chapiter instills 2-3 Herba Dendrobii pollen grain group；
(5) manage after pollinating：After pollination 10 days, the sucrose of its quality 1.5% is added in filtrate A, stirring obtains solution after melting
C, solution C presses 1:50 mass ratios are watered, and spray the Herba Dendrobii after pollinating 10 days.
2. a kind of Herba Dendrobii pollinating method according to claim 1, it is characterised in that the pollen grain of the step (1)
Collection after be placed on 6-8 DEG C at preserve 2 hours.
3. a kind of Herba Dendrobii pollinating method according to claim 1, it is characterised in that the pollen of the step (2) is carried
Body fluid is preserved 4 hours at 6-8 DEG C is placed on after preparing.
4. a kind of Herba Dendrobii pollinating method according to claim 1, it is characterised in that ultrasound is in the step (3)
Ultrasonic frequency is 22KHz in matter, and ultrasonic power is 250W, and sonication treatment time is 20 seconds, on the basis of 1.5L materials.
5. a kind of Herba Dendrobii pollinating method according to claim 1, it is characterised in that solution C is converted in the step (5)
After water, fountain height is 5kg/ mus.
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| CN201610989614.6A CN106577252A (en) | 2016-11-10 | 2016-11-10 | Dendrobium officinale pollination method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115462311A (en) * | 2022-10-21 | 2022-12-13 | 玉溪中烟种子有限责任公司 | Method for preparing composite pollen in liquid loading mode |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115462311A (en) * | 2022-10-21 | 2022-12-13 | 玉溪中烟种子有限责任公司 | Method for preparing composite pollen in liquid loading mode |
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Application publication date: 20170426 |