CN103891602A - Cross pollination method for dendrobium officinale - Google Patents
Cross pollination method for dendrobium officinale Download PDFInfo
- Publication number
- CN103891602A CN103891602A CN201410126179.5A CN201410126179A CN103891602A CN 103891602 A CN103891602 A CN 103891602A CN 201410126179 A CN201410126179 A CN 201410126179A CN 103891602 A CN103891602 A CN 103891602A
- Authority
- CN
- China
- Prior art keywords
- dendrobium
- produce
- pollen
- dendrobium officinale
- flowers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a simple and efficient cross pollination method for dendrobium officinale. The method comprises the following steps: (1) collecting pollen; (2) preparing dendrobium officinale flower juice; (3) preparing a pollen suspension; (4) pollinating; and (5) performing management at the later stage of fruit setting. A dendrobium officinale pollen load suspension is obtained by performing ultrasonic homogenization on pollen grains and the dendrobium officinale flower juice, and dendrobium officinale flowers are pollinated by the dendrobium officinale pollen load suspension by using a rubber-head burette, so that damages to stigmas of female parents are avoided, the fruit setting rate is high (over 98.2 percent), inane fruits are prevented, mature dendrobium officinale capsules can be obtained by sitting for 120 days, and fruits are uniform and plump. The method has the characteristics of easiness, convenience, high efficiency and high operability, the pollinating efficiency is 5-10 times that of a conventional pollinating method, the pollinating efficiency of dendrobium officinale is increased greatly, the application value is extremely high, and a novel high-speed and effective way is provided for hand pollination of dendrobium officinale.
Description
Technical field
The present invention relates to a kind of hybridizing propagation process of rare medicinal plant, specifically a kind of hybridization pollination method of dendrobium candidum.
Background technology
Dendrobium candidum (Demdrobium of ficinale) is the orchid family Dendrobium herbaceos perennial, is the famous and precious rare traditional Chinese medicine of China tradition, have nourishing the stomach to improve the production of body fluid, nourishing Yin and clearing heat, improve the effects such as body immunity.At present, dendrobium candidum has been listed in special-protection-by-the-State medicinal plant, the world's two class protective plants.
Produce dendrobium flowers makes the fruiting rate of dendrobium candidum under natural conditions extremely low because of its special physiological structure, dendrobium candidum general May is full-bloom stage, florescence 10 ~ 15d, its fruit is capsule, when ripe, easily split, seed ballooning is scattered, and the kind intracutaneous of these tiny seeds is only containing immature embryo,, containing endosperm, natural propagation is very not difficult.Along with the development of modern plants tissue culture technology, become quite ripe bred in the sprouting that people carry out stem of noble dendrobium embryo by tissue culture technology.The acquisition of stem of noble dendrobium embryo has become one of factor of dendrobium candidum industrialized development, the at present acquisition of stem of noble dendrobium embryo is mainly pollinated and is obtained by Traditional Man, Traditional Man pollinating method have operating technology require high, place property require harsh, labour intensity is large, production efficiency is low, fruit-setting rate is low and the shortcoming such as empty really rate height.As the Chinese invention patent application " hybridization pollination method of dendrobium officinale " that on September 23rd, 2009, disclosed application number was 200910010107.3, a kind of stem of noble dendrobium hybridization technique is provided, but it needs manually with tweezers, the pollen grain of male parent to be delivered in maternal column cap nest in pollinating process, when operation, easily column cap is caused to physical damnification, and adopt this method fruit-setting rate is the highest and be no more than 80.6%.Therefore simple hybridization pollination method of dendrobium officinale efficiently need further research and development.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of hybridization pollination method of simple and direct efficient, workable dendrobium candidum, can greatly improve the efficiency of dendrobium candidum pollination.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of hybridization pollination method of dendrobium candidum, is characterized in that comprising the following steps:
1) collection of pollen: the clip florescence, at the produce dendrobium flowers of 4 ~ 7d, is collected respectively pollen grain and produce dendrobium flowers;
2) preparation of produce dendrobium flowers juice: produce produce dendrobium flowers juice after the produce dendrobium flowers cleaning of above-mentioned collection is dried;
3) preparation of pollen suspension: the pollen grain that step 1) is collected joins step 2) in the produce dendrobium flowers juice produced after, utilize ultrasonic homogeneous to make dendrobium candidum pollen grain group suspension;
4) pollination: use glue head dropper that dendrobium candidum pollen grain group suspension is pollinated to the produce dendrobium flowers of 4 ~ 7d in full bloom, every dendrobium candidum chapiter guarantees to splash into 2 ~ 4 dendrobium candidum pollen grain groups;
5) final-period management of bearing fruit.
As improvement, the detailed process of the collection of the pollen of described step 1) is: the clip florescence is at the produce dendrobium flowers of 4 ~ 7d, dispersed to clean ceramic whiteware dish, in shaking table, process after 30 ~ 60min in 25 ~ 28 ℃, 180 ~ 230r/min, collect respectively pollen grain and produce dendrobium flowers in ceramic whiteware dish.
As improvement, described step 2) the detailed process of preparation of produce dendrobium flowers juice be: the produce dendrobium flowers after cleaning is dried is put into Ice-making machine and is stirred 2 ~ 3min, then obtain after filtrate by 120 ~ 160 object screen clothes, in filtrate, add the deionized water of 0.5 ~ 1.5 times of filtrate volume to obtain produce dendrobium flowers juice.
Improve, the final-period management of bearing fruit of described step 5) is again: after the 10 ~ 15d that bears fruit, add up fruit-setting rate, the 15 ~ 20d that bears fruit carries out fruit thinning to guarantee the capsule required normal nutrient of growing, and final-period management manages by normal mode.120d can obtain ripe stem of noble dendrobium capsule.
Preferably, in described step 3), in ultrasonic homogeneous, ultrasonic frequency is 10 ~ 40KHz, and ultrasonic power is 100 ~ 400W, and the ultrasonic processing time is 30 ~ 45 seconds, take 1L material as benchmark.
Finally, in described step 3), ultrasonic homogeneous adopts ultrasonic homogeneous instrument, supersonic oscillations instrument or ultrasonic washing instrument.
Compared with prior art, the invention has the advantages that: the present invention will obtain dendrobium candidum pollen grain group suspension after pollen grain and the ultrasonic homogeneous of produce dendrobium flowers juice, use glue head dropper that dendrobium candidum pollen grain group suspension is pollinated to produce dendrobium flowers, avoid the damage to maternal column cap, and fruit-setting rate high (more than 98.2%), the empty fruit of nothing exist, the 120d that bears fruit can obtain ripe stem of noble dendrobium capsule, and fruit is evenly full.The present invention has simple and direct efficient, workable feature, pollination efficiency is traditional pollinating method 5 ~ 10 times, greatly improved the efficiency of dendrobium candidum pollination, using value is high, for an effective new way of high speed has been opened up in the artificial pollination of dendrobium candidum.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment mono-: the clip florescence, at the produce dendrobium flowers of 4d, in dispersed extremely clean ceramic whiteware dish, is placed in 25 ℃, processes after 30min in the shaking table of 180r/min, collects pollen grain and produce dendrobium flowers in ceramic whiteware dish; After the produce dendrobium flowers cleaning of collecting is dried, put into Ice-making machine and stir 3min, then obtain after filtrate by 160 object screen clothes, in filtrate, add the deionized water of 1.5 times of filtrate volumes to make produce dendrobium flowers juice; Then the pollen grain of acquisition is joined in produce dendrobium flowers juice, be placed in the ultrasonic processing of ultrasonic washing instrument 30s, ultrasonic frequency 20KHz, ultrasonic power 200W, take 1L material as benchmark, utilizes ultrasonic homogeneous to make dendrobium candidum pollen grain group suspension; Use afterwards glue head dropper to utilize dendrobium candidum pollen grain group suspension to pollinate to the produce dendrobium flowers of 7d in full bloom, every dendrobium candidum chapiter guarantees to splash into 4 dendrobium candidum pollen grain groups, on average can pollinate for each person every day about 2000 flowers, be 5 ~ 7 times of traditional pollination efficiency; Bear fruit after 10d and add up fruit-setting rate, fruit-setting rate is to carry out fruit thinning after 98.2%, 15d, and the later stage carries out managing according to normal maintenance method.After 120d, obtain ripe fruit pod, its empty fruit rate is 0%.
Embodiment bis-: the clip florescence, at the produce dendrobium flowers of 7d, in dispersed extremely clean ceramic whiteware dish, is placed in 27 ℃, processes after 60min in the shaking table of 230r/min, collects pollen grain and produce dendrobium flowers in ceramic whiteware dish; After the produce dendrobium flowers cleaning of collecting is dried, put into Ice-making machine and stir 3min, then obtain after filtrate by 120 object screen clothes, in filtrate, add the deionized water of 0.5 times of filtrate volume to make produce dendrobium flowers juice; Then the pollen grain of acquisition is joined in produce dendrobium flowers juice, be placed in the ultrasonic processing of ultrasonic homogeneous instrument 45s, ultrasonic frequency 30KHz, ultrasonic power 300W, take 1L material as benchmark, makes dendrobium candidum pollen grain group suspension; Use afterwards glue head dropper to utilize dendrobium candidum pollen grain group suspension to pollinate to the produce dendrobium flowers of 5d in full bloom, every dendrobium candidum chapiter guarantees to splash into 3 dendrobium candidum pollen grain groups, on average can pollinate for each person every day about 2000 flowers, be 5 ~ 7 times of traditional pollination efficiency; Bear fruit after 15d and add up fruit-setting rate, fruit-setting rate is to carry out fruit thinning after 99.7%, 20d, and the later stage carries out managing according to normal maintenance method.After 120d, obtain ripe fruit pod, its empty fruit rate is 0%.
Embodiment tri-: get the produce dendrobium flowers of florescence at 5d, in dispersed extremely clean ceramic whiteware dish, be placed in 26 ℃, process after 45min in the shaking table of 200r/min, collect pollen grain and produce dendrobium flowers in ceramic whiteware dish; After the produce dendrobium flowers cleaning of collecting is dried, put into Ice-making machine and stir 2.5min, then obtain after filtrate by 140 object screen clothes, in filtrate, add the deionized water of 1 times of filtrate volume to make produce dendrobium flowers juice; Then the pollen grain of acquisition is joined in produce dendrobium flowers juice, be placed in the ultrasonic processing of ultrasonic washing instrument 40s, ultrasonic frequency 40KHz, ultrasonic power 400W, take 1L material as benchmark, makes dendrobium candidum pollen grain group suspension; Use afterwards glue head dropper to utilize dendrobium candidum pollen grain group suspension to pollinate, every dendrobium candidum chapiter guarantees to splash into 2 dendrobium candidum pollen grain groups, on average can pollinate for each person every day about 3000 flowers, is 7 ~ 10 times of traditional pollination efficiency; Bear fruit after 12d and add up fruit-setting rate, fruit-setting rate is to carry out fruit thinning after 99.5%, 18d, and the later stage carries out managing according to normal maintenance method.After 120d, obtain ripe fruit pod, its empty fruit rate is 0%.
Claims (6)
1. a hybridization pollination method for dendrobium candidum, is characterized in that comprising the following steps:
1) collection of pollen: the clip florescence, at the produce dendrobium flowers of 4 ~ 7d, is collected respectively pollen grain and produce dendrobium flowers;
2) preparation of produce dendrobium flowers juice: produce produce dendrobium flowers juice after the produce dendrobium flowers cleaning of above-mentioned collection is dried;
3) preparation of pollen suspension: the pollen grain that step 1) is collected joins step 2) in the produce dendrobium flowers juice produced after, utilize ultrasonic homogeneous to make dendrobium candidum pollen grain group suspension;
4) pollination: use glue head dropper that dendrobium candidum pollen grain group suspension is pollinated to the produce dendrobium flowers of 4 ~ 7d in full bloom, every dendrobium candidum chapiter guarantees to splash into 2 ~ 4 dendrobium candidum pollen grain groups;
5) final-period management of bearing fruit.
2. hybridization pollination method according to claim 1, it is characterized in that: the detailed process of the collection of the pollen of described step 1) is: the clip florescence is at the produce dendrobium flowers of 4 ~ 7d, dispersed to clean ceramic whiteware dish, in shaking table, process after 30 ~ 60min in 25 ~ 28 ℃, 180 ~ 230r/min, collect respectively pollen grain and produce dendrobium flowers in ceramic whiteware dish.
3. hybridization pollination method according to claim 1 and 2, it is characterized in that: described step 2) the detailed process of preparation of produce dendrobium flowers juice be: the produce dendrobium flowers after cleaning is dried is put into Ice-making machine and is stirred 2 ~ 3min, then obtain after filtrate by 120 ~ 160 object screen clothes, in filtrate, add the deionized water of 0.5 ~ 1.5 times of filtrate volume to obtain produce dendrobium flowers juice.
4. hybridization pollination method according to claim 3, it is characterized in that: the final-period management of bearing fruit of described step 5) is: after the 10 ~ 15d that bears fruit, add up fruit-setting rate, 15 ~ the 20d that bears fruit carries out fruit thinning to guarantee the capsule required normal nutrient of growing, and final-period management manages by normal mode.
5. hybridization pollination method according to claim 3, is characterized in that: in described step 3), in ultrasonic homogeneous, ultrasonic frequency is 10 ~ 40KHz, and ultrasonic power is 100 ~ 400W, and the ultrasonic processing time is 30 ~ 45 seconds, take 1L material as benchmark.
6. hybridization pollination method according to claim 1, is characterized in that: in described step 3), ultrasonic homogeneous adopts ultrasonic homogeneous instrument, supersonic oscillations instrument or ultrasonic washing instrument.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410126179.5A CN103891602B (en) | 2014-04-01 | 2014-04-01 | Cross pollination method for dendrobium officinale |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410126179.5A CN103891602B (en) | 2014-04-01 | 2014-04-01 | Cross pollination method for dendrobium officinale |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103891602A true CN103891602A (en) | 2014-07-02 |
| CN103891602B CN103891602B (en) | 2015-06-24 |
Family
ID=50983441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410126179.5A Active CN103891602B (en) | 2014-04-01 | 2014-04-01 | Cross pollination method for dendrobium officinale |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103891602B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577252A (en) * | 2016-11-10 | 2017-04-26 | 潘显船 | Dendrobium officinale pollination method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008072902A (en) * | 2006-09-19 | 2008-04-03 | Akita Prefecture | Liquid pollination method and preparation using the same |
| CN102318549A (en) * | 2011-08-16 | 2012-01-18 | 南京农业大学 | Method with effects of saving cost and improving efficiency for liquid pollination of pear tree |
| CN102657078A (en) * | 2012-05-25 | 2012-09-12 | 苏州神元生物科技有限公司 | Method of artificial pollination for Dendrobium candidum |
| CN103109737A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Pollen liquid for high yield of hazelnut fruit and cultivation and pollination of hybrid hazel variety, and preparation method thereof |
| CN103408355A (en) * | 2013-07-16 | 2013-11-27 | 南京师范大学 | Method and special treatment agent for increasing pollination seed setting rate of dendrobium officinale and dendrobium moniliforme |
-
2014
- 2014-04-01 CN CN201410126179.5A patent/CN103891602B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008072902A (en) * | 2006-09-19 | 2008-04-03 | Akita Prefecture | Liquid pollination method and preparation using the same |
| CN102318549A (en) * | 2011-08-16 | 2012-01-18 | 南京农业大学 | Method with effects of saving cost and improving efficiency for liquid pollination of pear tree |
| CN102657078A (en) * | 2012-05-25 | 2012-09-12 | 苏州神元生物科技有限公司 | Method of artificial pollination for Dendrobium candidum |
| CN103109737A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Pollen liquid for high yield of hazelnut fruit and cultivation and pollination of hybrid hazel variety, and preparation method thereof |
| CN103408355A (en) * | 2013-07-16 | 2013-11-27 | 南京师范大学 | Method and special treatment agent for increasing pollination seed setting rate of dendrobium officinale and dendrobium moniliforme |
Non-Patent Citations (2)
| Title |
|---|
| SHARMISTHA NAIK, ET AL.: "spray pollination : an efficient and labour saving method for kiwifruit(Actinidia deliciosa A. Chev.) production", 《JOURNAL OF APPLIED HORTICULTURE》, vol. 15, no. 3, 31 December 2013 (2013-12-31), pages 202 - 206 * |
| 金银兵: "铁皮石斛的生物学特性与开花授粉技术研究", 《安徽农业科学》, vol. 37, no. 11, 31 December 2009 (2009-12-31), pages 5280 - 5282 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577252A (en) * | 2016-11-10 | 2017-04-26 | 潘显船 | Dendrobium officinale pollination method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103891602B (en) | 2015-06-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102100176B (en) | Method for efficiently inducing jujube tree 2n pollen | |
| CN101213940A (en) | Fast replication method for dendrobium | |
| CN103190343B (en) | Key technology of organic additive for roxburgh anoectochilus terminal bud industrialization intermediate propagation | |
| CN105706874A (en) | Method for raising moringa seedlings through cutting | |
| CN102960239A (en) | Cultivating method of red-flesh apples | |
| CN108739383A (en) | A kind of tuber of pinellia quick breeding method for tissue culture | |
| CN107810855A (en) | The plant modification method of salvia miltiorrhiza detoxification seedling | |
| CN102228005A (en) | Pinellia ternate tissue culture one-step speciation method | |
| CN101874472B (en) | Tissue culture method of Pitaya | |
| CN103270948B (en) | Two polyploid centella asiatica varieties and cultivation method thereof | |
| CN117730779B (en) | Tissue culture method for promoting rapid rooting of Rosa roxburghii | |
| CN102124942A (en) | Tobacco pollen medium as well as preparation method and application thereof | |
| CN103891602B (en) | Cross pollination method for dendrobium officinale | |
| CN105557514A (en) | Low temperature preservation method for roxburgh anoectochilus terminal bud germplasm resources | |
| CN103651146B (en) | The method of yam test tube microtubers is begged in a kind of production | |
| CN104663430A (en) | Cultivation method for purple hot peppers capable of being eaten raw and being high in anthocyanin content | |
| CN117178885A (en) | Dian white bead tissue culture method | |
| CN110291989A (en) | A kind of method for building up in the sterile leaf source for gingkgo callus induction | |
| CN105613278A (en) | Artificial hybridization and pollination method for Chinese soapberry fruits | |
| CN104255497A (en) | Method for immediately propagating pholidota cantonensis by using pholidota cantonensis rhizomes as explants | |
| CN208940433U (en) | Banana inhales bud and roots out device | |
| CN107996387A (en) | A kind of hybridization pollination method of dendrobium candidum | |
| CN109122326A (en) | A kind of aseptic seeding and method for tissue culture of roxburgh anoectochilus terminal bud | |
| CN104969852A (en) | Method of pollinating strawberries in greenhouse with bumblebees | |
| CN104472346A (en) | Effective sexual hybridization technology of abelmoschus esculentus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |