CN106565359A - Biofertilizer for inhibiting root-knot nematodes, and preparation method thereof - Google Patents
Biofertilizer for inhibiting root-knot nematodes, and preparation method thereof Download PDFInfo
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- CN106565359A CN106565359A CN201610813259.7A CN201610813259A CN106565359A CN 106565359 A CN106565359 A CN 106565359A CN 201610813259 A CN201610813259 A CN 201610813259A CN 106565359 A CN106565359 A CN 106565359A
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- bio
- feritlizer
- bacillus
- water
- root
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention provides a biofertilizer for inhibiting root-knot nematodes. The biofertilizer comprises the following ingredients: 15-20wt% of bacillus subtilis, 15-20% of bacillus latersprorus, 15-20% of bacillus mucilaginosus, 0.5-3% of stabilizer, 30-40% of starfish extracting solution and the balance of water. The biofertilizer has the efficiencies on inhibiting the root-knot nematodes and protecting the growth environment of crop roots and has a good effect on reducing rotted roots and dead seedlings and preventing soil-borne diseases. The preparation method for the biofertilizer is simple, green and environmentally friendly, auxiliary raw materials are added according to practical crop requirements, and a fertilizer with composite functions is prepared.
Description
Technical field
The present invention relates to a kind of bio-feritlizer, more particularly to a kind of bio-feritlizer for suppressing root-knot nematode and its preparation side
Method.
Background technology
Root-knot nematode is a kind of omnivorousness plant pathogeny line insect of height specialized form, mainly there is high bow root-knot nematode, Semen arachidis hypogaeae
Root-knot nematode, M hapla, Meloidogyne incognita, javanese root knot nematode and Radix Betae root-knot nematode etc., host range is wide
It is general, enter 30 various vegetables such as melon, solanaceous vegetables, beans and Radix Raphani, calabash Radix Raphani, Caulis et Folium Lactucae sativae, Chinese cabbage, moreover it is possible to propagate some funguses and antibacterial
Sexually transmitted disease (STD) does harm to.The root of the various vegetables of root-knot nematode main harm, shows as lateral root and fibrous root more normally increases, and in the palpus of young root
The white root nodule that spherical or cone differs in size is formed on root, have in beads shape, killed strain overground part growth is short and small, slow
Slowly, leaf color exception, it is as a result few, yield poorly, or even cause plant to do sth. in advance death.
The method for suppressing root-knot nematode is usually agricultural measures, Agro-chemicals control, microbial control and electric anti-method for the treatment of etc..
Agricultural measures predominantly from taeniasis Seedling without worm seedlings and soil, being rejected during transplanting or removing in " root nodule ", remove taeniasis residuum, force down
Insect density, taeniasis root should burn after drying, and plough deeply soil, and table soil is turned to into less than 25 centimetres, can mitigate insect pest generation, crop rotation
Insect prevention.There are many fields in nematicide, replant anti-（It is resistance to）Worm crop such as grass tree section, Herba Alii fistulosi, Bulbus Allii, Folium Allii tuberosi, Fructus Capsici, Caulis et Folium Brassicae capitatae, cauliflower etc. or
Plantation aquatic vegetable, can mitigate the generation of nematicide, high（It is low）Temperature suppression worm, using summer high temperature leisure season, ridging is poured water and covers ground
Film, airtight awning room two weeks, freezing upturned soil etc. using winter low temperature can suppress nematicide to occur, and labour force is larger, and has the presence of recurrence, effect
Fruit is not good；Agro-chemicals control can select chemical particle agent, ploughs after spreading fertilizer over the fields and buries, and injure larger to crops, and not environmentally；
There is larger danger in the anti-method for the treatment of of electricity；Microbial control is that comparison is environmentally friendly, safely and effectively method, such as bio-feritlizer.
In view of above-mentioned defect, the design people, positive in addition research and innovation, to founding a kind of suppression root-knot nematode
Bio-feritlizer and preparation method thereof so as to more the value in industry.
The content of the invention
To solve above-mentioned technical problem, it is an object of the invention to provide the bio-feritlizer and its system of a kind of suppression root-knot nematode
Preparation Method.
The bio-feritlizer of the suppression root-knot nematode of the present invention, including mass percent is respectively the bacillus subtilis of 15-20%
The Asterias amurensis Lutken of bacterium, the side bacillus cereuss of 15-20%, the colloid bacillus cereus of 15-20%, the stabilizer of 0.5-3% and 30-40% is extracted
Liquid, remaining is water, wherein, bacillus subtilises, side bacillus cereuss, colloid bacillus cereus are 2 × 1010Individual viable bacteria/gram, hay
Bacillus cereuss deposit number is CGMCC1.3358, and bacillus cereuss deposit number in side is CGMCC No. 8482, colloid bacillus cereus
Deposit number is CGMCC 1.153.
Further, the bio-feritlizer is respectively the bacillus subtilises of 15-20%, 15%-20% by mass percent
Side bacillus cereuss, the colloid bacillus cereus of 15%-20%, the stabilizer of 0.5-3%, the Asterias amurensis Lutken extracting solution of 30-40% and 5-20%PH
6.5-7.5 sterilized water composition.
Further, according to the needs of targeted efficacy, product forms and using method in the bio-feritlizer, also including auxiliary
Help raw material.
Further, the auxiliary material is seaweed extracted liquor or hoof tips protein liquid.
Further, the water is sterilized water.
Further, the pH value of the sterilized water is 6.5-7.5.
Further, the stabilizer is the one kind in EDTA, flavonoid and coumarin.
The preparation method of the bio-feritlizer of the present invention, comprises the following steps：
（1）Mass percent is added in agitator tank for the Asterias amurensis Lutken extracting solution of 30-40% and is stirred, add acid solution（Hydrochloric acid/
Sulphuric acid/citric acid）Or aqueous slkali（NaOH/KOH/）PH value is adjusted to 7-8；
（2）By 2 × 1010Individual viable bacteria/gram bacillus subtilises, 2 × 1010Individual viable bacteria/gram side bacillus cereuss, 2 × 1010It is individual
Viable bacteria/gram colloid bacillus cereus and stabilizer mixture and stir；Wherein, the mass percent of each component is respectively 15-
20% bacillus subtilises, the stabilizer of the side bacillus cereuss, the colloid bacillus cereus of 15-20% and 0.5-3% of 15-20%；Its
Middle stabilizer is the one kind in EDTA, flavonoid and coumarin.
（3）By step（1）With（2）Liquid blending, and 25-30 DEG C, aerobic, condition of normal pressure bottom fermentation 48-72 hours,
It is the quantity of metabolite with increase viable count and mycobacterial derivatives, then adds the sterilized water adjustment that pH value is 6.5-7.5 total
Weight stirs to 100%, obtains the bio-feritlizer, final products be living bacteria count >=1,000,000,000/gram, pH value 6.5-
7.5 brown color water preparations.
Further, step（3）In, before adding water, according to the needs of targeted efficacy, product forms and using method, also
One or more auxiliary material is added, the auxiliary material is seaweed extracted liquor or hoof tips protein liquid.
A kind of using method of bio-feritlizer of the present invention, the using method is to spray, rush and apply or drip irrigation.
By such scheme, the present invention at least has advantages below：
The bio-feritlizer of the present invention can suppress root-knot nematode, cover crop root growth environment, to reducing by rotten dead seedling and preventing
Soil-borne disease has good effect；And the bio-feritlizer preparation method of the present invention is simple, environmental protection can also be according to actual crop
Need to add auxiliary material, prepare a kind of complex function fertilizer.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of description, described in detail as after with presently preferred embodiments of the present invention below.
Specific embodiment
With reference to embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for
The present invention is illustrated, but is not limited to the scope of the present invention.
Embodiment 1
Suppress the bio-feritlizer preparation method of root-knot nematode, comprise the following steps：
（1）The Asterias amurensis Lutken extracting solution of mass percent 30% is added in agitator tank and is stirred, add hydrochloric acid to adjust pH value to 7；
（2）By 2 × 1010Individual viable bacteria/gram bacillus subtilises, 2 × 1010Individual viable bacteria/gram side bacillus cereuss, 2 × 1010It is individual
Viable bacteria/gram colloid bacillus cereus and stabilizer mixture and stir；Wherein, the mass percent of each component is respectively 20%
Bacillus subtilises, 20% side bacillus cereuss, 20% colloid bacillus cereus and 0.5% stabilizer EDTA；
（3）By step（1）With（2）Liquid blending, and 30 DEG C, aerobic, condition of normal pressure bottom fermentation 48 hours, to increase viable bacteria
Number and mycobacterial derivatives are the quantity of metabolite, then add the sterilized water that pH value is 6.5 to adjust gross weight to 100%, and are stirred
Mix uniform, obtain bio-feritlizer.
Final products be living bacteria count >=2,000,000,000/gram, pH value 6.5-7.5 brown color water preparations.The fertilizer is when in use
Every mu of 5-10 kilogram of pouring root or punching are applied.Bio-feritlizer manufactured in the present embodiment is applied in Radix Dauci Sativae with 8 kgs/acre of usage amount
In seedling stage, compare with blank control group, rotten death rate for applying Radix Dauci Sativae after this product declines 82%, and yield increases by 28%.
Embodiment 2
Suppress the bio-feritlizer preparation method of root-knot nematode, comprise the following steps：
（1）The Asterias amurensis Lutken extracting solution of mass percent 40% is added in agitator tank and is stirred, add citric acid to adjust pH value to 8；
（2）By 2 × 1010Individual viable bacteria/gram bacillus subtilises, 2 × 1010Individual viable bacteria/gram side bacillus cereuss, 2 × 1010It is individual
Viable bacteria/gram colloid bacillus cereus and stabilizer mixture and stir；Wherein, the mass percent of each component is respectively 15%
Bacillus subtilises, 15% side bacillus cereuss, 15% colloid bacillus cereus and 3% stabilizer coumarin；
（3）By step（1）With（2）Liquid blending, and 25 DEG C, aerobic, condition of normal pressure bottom fermentation 72 hours, to increase viable bacteria
Number and mycobacterial derivatives be metabolite quantity, add auxiliary material seaweed extracted liquor, then plus pH value be 7.5 it is aseptic
Water adjusts gross weight to 100%, and stirs, and obtains bio-feritlizer.
Final products be living bacteria count >=1,000,000,000/gram, pH value 6.5-7.5 brown color water preparations.The fertilizer is when in use
Every mu of 50-100 milliliter foliage-spray, or every mu of 1-2 kilogram of punching applies or drip irrigation.Bio-feritlizer manufactured in the present embodiment is with 60 millis
The usage amount that liter/mu is sprayed is applied to Fructus Solani melongenae seedling stage, and relative to blank control group, the Fructus Solani melongenae upgrowth situation of this product administration group is more
Good, leaf is loose, and harvest time experimental group yield increases by 26%.
The above is only the preferred embodiment of the present invention, is not limited to the present invention, it is noted that for this skill
For the those of ordinary skill in art field, on the premise of without departing from the technology of the present invention principle, can also make it is some improvement and
Modification, these are improved and modification also should be regarded as protection scope of the present invention.
Claims (10)
1. it is a kind of suppress root-knot nematode bio-feritlizer, it is characterised in that：The bio-feritlizer is respectively including mass percent
The bacillus subtilises of 15-20%, the side bacillus cereuss of 15-20%, the colloid bacillus cereus of 15-20%, the stabilizer of 0.5-3% and
The Asterias amurensis Lutken extracting solution of 30-40%, remaining is water；Wherein, bacillus subtilises deposit number be CGMCC1.3358, side bacillus cereuss
Deposit number is CGMCC No. 8482, and colloid bacillus cereus deposit number is CGMCC 1.153.
2. bio-feritlizer according to claim 1, it is characterised in that：The bio-feritlizer is respectively by mass percent
The bacillus subtilises of 15-20%, the side bacillus cereuss of 15%-20%, the colloid bacillus cereus of 15%-20%, 0.5-3% stablize
Agent, the Asterias amurensis Lutken extracting solution of 30-40% and 5-20% water composition.
3. bio-feritlizer according to claim 1, it is characterised in that：Also include auxiliary material.
4. bio-feritlizer according to claim 3, it is characterised in that：The auxiliary material is seaweed extracted liquor or hoof tips egg
White liquor.
5. bio-feritlizer according to claim 1, it is characterised in that：The water is sterilized water.
6. bio-feritlizer according to claim 5, it is characterised in that：The pH value of the sterilized water is 6.5-7.5.
7. bio-feritlizer according to claim 1, it is characterised in that：The stabilizer is EDTA, flavonoid and coumarin
In one kind.
8. the preparation method of the arbitrary described bio-feritlizer of a kind of claim 1-7, it is characterised in that comprise the following steps：
（1）Asterias amurensis Lutken extracting solution is stirred and pH value is adjusted to 7-8；
（2）Bacillus subtilises, side bacillus cereuss, colloid bacillus cereus and stabilizer are mixed；
（3）By step（1）With（2）Liquid blending, fermentation, the adjustment gross weight that adds water and mixes to 100%, obtains the biology
Fertilizer.
9. preparation method according to claim 8, it is characterised in that：Step（3）In, before adding water, it is additionally added auxiliary former
Material.
10. a kind of using method of bio-feritlizer, it is characterised in that：The bio-feritlizer is that claim 1-9 is arbitrary described
Bio-feritlizer, the using method is to spray, rush and apply or drip irrigation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610813259.7A CN106565359A (en) | 2016-09-10 | 2016-09-10 | Biofertilizer for inhibiting root-knot nematodes, and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610813259.7A CN106565359A (en) | 2016-09-10 | 2016-09-10 | Biofertilizer for inhibiting root-knot nematodes, and preparation method thereof |
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| CN106565359A true CN106565359A (en) | 2017-04-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610813259.7A Pending CN106565359A (en) | 2016-09-10 | 2016-09-10 | Biofertilizer for inhibiting root-knot nematodes, and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108130304A (en) * | 2018-02-10 | 2018-06-08 | 厦门昶青生物科技有限公司 | Bacillus composite bacterial liquid and preparation method thereof |
| CN110200027A (en) * | 2019-05-18 | 2019-09-06 | 山东省农业科学院植物保护研究所 | A kind of biological control method of groundnut root knot nematode disease |
| CN110278969A (en) * | 2019-05-18 | 2019-09-27 | 山东省农业科学院植物保护研究所 | A kind of complex micro organism fungicide and preparation method thereof for preventing and treating peanut root-knot nematode |
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|---|---|---|---|---|
| CN103420719A (en) * | 2013-07-22 | 2013-12-04 | 张文举 | Novel continuous-cropping resistant and dead seedling preventing bio-fertilizer |
| CN103420692A (en) * | 2013-07-29 | 2013-12-04 | 张文举 | Novel special bio-fertilizer applied in crop transplanting and fix planting period |
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2016
- 2016-09-10 CN CN201610813259.7A patent/CN106565359A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103420719A (en) * | 2013-07-22 | 2013-12-04 | 张文举 | Novel continuous-cropping resistant and dead seedling preventing bio-fertilizer |
| CN103420692A (en) * | 2013-07-29 | 2013-12-04 | 张文举 | Novel special bio-fertilizer applied in crop transplanting and fix planting period |
Non-Patent Citations (2)
| Title |
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| 廖金铃，彭德良，段玉玺主编: "《中国线虫学研究 第2卷》", 31 July 2008, 中国农业科学技术出版社 * |
| 李俊良，梁斌主编: "《设施蔬菜微灌施肥工程与技术》", 30 April 2016, 中国农业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108130304A (en) * | 2018-02-10 | 2018-06-08 | 厦门昶青生物科技有限公司 | Bacillus composite bacterial liquid and preparation method thereof |
| CN110200027A (en) * | 2019-05-18 | 2019-09-06 | 山东省农业科学院植物保护研究所 | A kind of biological control method of groundnut root knot nematode disease |
| CN110278969A (en) * | 2019-05-18 | 2019-09-27 | 山东省农业科学院植物保护研究所 | A kind of complex micro organism fungicide and preparation method thereof for preventing and treating peanut root-knot nematode |
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Application publication date: 20170419 |