CN106561684A - Bactericidal composition containing podophyllotoxin - Google Patents
Bactericidal composition containing podophyllotoxin Download PDFInfo
- Publication number
- CN106561684A CN106561684A CN201610969235.0A CN201610969235A CN106561684A CN 106561684 A CN106561684 A CN 106561684A CN 201610969235 A CN201610969235 A CN 201610969235A CN 106561684 A CN106561684 A CN 106561684A
- Authority
- CN
- China
- Prior art keywords
- podophyllotoxin
- avicularin
- bactericidal composition
- weight
- composition containing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a bactericidal composition containing podophyllotoxin. The bactericidal composition contains the podophyllotoxin and avicularin, and the ratio of parts by weight of the podophyllotoxin and the avicularin is between 1:1 and 20:1. The bactericidal composition is discovered to have a very good effect of synergistically preventing and treating verticillium wilt by virtue of compatibility of the podophyllotoxin and the avicularin in a specific proportion, and the synergistic effect of the bactericidal composition is quite obvious particularly when the ratio of the parts by weight of the podophyllotoxin and the avicularin is between 15:1 and 17:1.
Description
Technical field
The present invention relates to a kind of medicament, specifically a kind of bactericidal composition containing podophyllotoxin, belong to agricultural technology field.
Background technology
Podophyllotoxin (CAS 518-28-5) is from XIAONIE section may apple --- the root of Chinese common sinopodophyllum rhi-zome (also known as chicken tongue element)
With the wooden lipid antitumor composition extracted in stem.It is for white crystalline powder, odorless, there is hygroscopicity.Chinese common sinopodophyllum rhi-zome is mainly distributed
The ground such as the Gansu Province in China.The podophyllotoxin extracted from Chinese common sinopodophyllum rhi-zome (also known as chicken tongue element) can make unguentum and tincture.
Podophyllotoxin can effectively suppress herpesviruss, suppress the mitosiss of cell mid-term.
Avicularin (CAS 572-30-5) source is the complete of polygonaceae plant Herba Polygoni Avicularis (Polygonum aviculare L.)
Grass.Structural formula is as follows：
Actual pesticide experience have shown that, repeats and single-minded applies a kind of reactive compound to prevent and treat harmful fungoid very
The fast selective of fungal bacterial strain will be caused in the case of many, be that reduction refractory fungal bacterial strain is selectively dangerous, at present generally
Harmful fungoid is prevented and treated using the mixture of different activities compound.By the reactive compound with the different mechanisms of action is entered
Row combination, can delaying drug resistance produce, reduce amount of application, reduce cost accounting, it is found by the applicant that podophyllotoxin and avicularin are one
Coordinate under certainty ratio for the effect for preventing verticillium wilt that there is potentiation.
The content of the invention
For the problem that above-mentioned prior art is present, it is an object of the invention to provide the Bactericidal composite containing podophyllotoxin
Thing, it is to compound the sterilization component of two kinds of different structures, the different mechanisms of action, is expanding prevention spectrum and raising drug effect
Effective way, it is often more important that avoid or delay generation of the disease to new composition resistance.To realize the purpose of the present invention, inventor
Podophyllotoxin is compounded with avicularin, for preventing and treating the disease of crop.
To achieve these goals, the technical solution used in the present invention is：It is a kind of to include two kinds of principle active components：Rhizoma Dysosmae Versipellis
Toxin and avicularin.
Described podophyllotoxin with the ratio of the parts by weight of avicularin is, 1:1-20:1.
The weight sum of described podophyllotoxin and avicularin accounts for the 1-80% of medicament gross weight.
Described bactericidal composition, said composition can be made into suspending agent, wettable powder, seed coat agent, aqueous emulsion, microemulsion
Agent, cream, granule, water dispersible granules, and can be needed using corresponding solvent, carrier and auxiliary agent according to exploitation.
Suitable solvent, auxiliary agent include：Toluene, dimethylbenzene, dichloromethane, chloroform, paraffin, kerosene, diesel oil, first
Alcohol, ethanol, propanol, butanol, amylalcohol, Hexalin, benzyl alcohol, Ketohexamethylene, isophorone, ketopyrrolidine, ethyl acetate, acetic acid fourth
One or more combination of ester, ethylene acetate, dimethyl sulfoxide etc..
Carrier include clay, bentonite, attapulgite, calcium sulfate, precipitated calcium carbonate, sucrose, industrial chitin, silica gel,
One or more combination of Pulvis Talci, white carbon, sericite in powder etc.；
Available auxiliary agent includes phosphoric acid salt, hydrochloric acid salt, lignosulphonateses, naphthalenesulfonate, phenolsulfonate
Class, succinic acid Sulfonateses, polystyrene-based phenol polyethenoxy ether sulfate, Pluronic PE 6800, molecular weight are 20000-
30000 macromolecular grafted copolymer, triphenyl vinyl phenol polyoxyethylene poly-oxygen propylene aether, polystyrene-based phenol polyethenoxy
Ethers, NPE class, polystyrene-based polyethenoxy ether class, alkyl naphthalene sulfonic acid salt, aliphatic alcohol polyethenoxy
Ethers, dialkyl succinylsuccinate Sulfonateses, branched fatty alcohol polyethenoxy ether class, alkyl polyoxyethylene ether class, carboxyl alkyl cellulose
Class, hydroxy alkyl cellulose class, Magnesiumaluminumsilicate, xanthan gum, poly- alkyl silicon ethylene oxide, phosphoric acid, glacial acetic acid, citric acid, suitable fourth
One kind in enedioic acid, dibutyl hydroxy methane, Butylated hydroxyanisole, tert-butylhydroquinone, propylgallate etc. or
Multiple combination.
The present invention is by having found there is collaboration preventing and treating well by podophyllotoxin and avicularin compatibility under specific ratio
The effect of verticillium wilt, the particularly ratio in podophyllotoxin and the parts by weight of avicularin are 15:1-17:Between 1, its collaboration preventing and treating
Verticillium wilt effect is apparent, and toxicity is less.
Specific embodiment
Embodiment 1
Podophyllotoxin with the ratio of the parts by weight of avicularin is, 17:1,
Described podophyllotoxin accounts for the 17% of medicament gross weight with the weight sum of avicularin, N-Methyl pyrrolidone
11%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#8%, water complement to 100%.
Embodiment 2
Podophyllotoxin with the ratio of the parts by weight of avicularin is, 15:1,
Described podophyllotoxin accounts for the 17% of medicament gross weight with the weight sum of avicularin, N-Methyl pyrrolidone
11%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#8%, water complement to 100%.
Embodiment 3
Podophyllotoxin with the ratio of the parts by weight of avicularin is, 10:1,
Described podophyllotoxin accounts for the 17% of medicament gross weight with the weight sum of avicularin, N-Methyl pyrrolidone
11%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#8%, water complement to 100%.
Embodiment 4
Podophyllotoxin with the ratio of the parts by weight of avicularin is, 8:1,
Described podophyllotoxin accounts for the 17% of medicament gross weight with the weight sum of avicularin, N-Methyl pyrrolidone
11%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#8%, water complement to 100%.
Embodiment 5
Podophyllotoxin with the ratio of the parts by weight of avicularin is, 5:1,
Described podophyllotoxin accounts for the 17% of medicament gross weight with the weight sum of avicularin, N-Methyl pyrrolidone
11%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#8%, water complement to 100%.
Embodiment 6
Podophyllotoxin with the ratio of the parts by weight of avicularin is, 2:1,
Described podophyllotoxin accounts for the 17% of medicament gross weight with the weight sum of avicularin, N-Methyl pyrrolidone
11%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#8%, water complement to 100%.
Embodiment 7
Podophyllotoxin with the ratio of the parts by weight of avicularin is, 1:1,
Described podophyllotoxin accounts for the 17% of medicament gross weight with the weight sum of avicularin, N-Methyl pyrrolidone
11%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#8%, water complement to 100%.
Embodiment 8
Podophyllotoxin with the ratio of the parts by weight of avicularin is, 1:2,
Described podophyllotoxin accounts for the 17% of medicament gross weight with the weight sum of avicularin, N-Methyl pyrrolidone
11%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#8%, water complement to 100%.
Embodiment 9
Podophyllotoxin with the ratio of the parts by weight of avicularin is, 1:6,
Described podophyllotoxin accounts for the 17% of medicament gross weight with the weight sum of avicularin, N-Methyl pyrrolidone
11%, calcium dodecyl benzene sulfonate 5%, agriculture breast 600#8%, water complement to 100%.
Laboratory test：
Subjects：Verticillium wilt pathogenic bacteria
According to the incidence of the whole strain blade of test grade scale investigation Fructus Solani melongenae, disease index and prevention effect are calculated.Will
Prevention effect is converted into niqueMin (y), and medicinal liquid is done height (μ g/ml) and is converted into logarithm value (x), calculates virulence with method of least square
Concentration EC50 in equation and suppression, calculates toxicity index level co-toxicity coefficient (CTC) of medicament according to the abundant methods of Sun Yun.
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agents EC50) * 100
Percentage composition+B medicament toxicity index * the mixtures of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixtures
The percentage composition co-toxicity coefficient (CTC) of middle B=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] *
100CTC≤80, compositionss show as antagonism, 80 ＜ CTC ＜ 120, and compositionss show as summation action, CTC >=120, group
Compound shows as potentiation.
1 podophyllotoxin of table：Avicularin and its different ratio are compounded to verticillium wilt toxicity test interpretation of result.
A is podophyllotoxin, and B is avicularin
| Reagent treatment | ATI | TTI | Co-toxicity coefficient CTC |
| A：B(20:1) | 90.2 | 88.2 | 102.3 |
| A：B(17:1) | 179.6 | 83.5 | 215.1 |
| A：B(15:1) | 152.8 | 79.4 | 192.4 |
| A：B(10:1) | 79.1 | 74.1 | 106.7 |
| A：B(5:1) | 64.3 | 71.8 | 89.6 |
| A：B(3:1) | 66.1 | 70.1 | 94.3 |
| A：B(1:1) | 59.7 | 62.6 | 95.4 |
| A：B(1:6) | 55.9 | 55.2 | 101.2 |
From the measurement result of table 1, work as podophyllotoxin：Avicularin proportioning is 15:1-17:When between 1, co-toxicity coefficient is equal
It is much higher than 190, with obvious potentiation, and other ratios mix, and co-toxicity coefficient is not obvious.
Field efficacy checking test
Test method：At the initial stage of a disease, first time spraying being carried out immediately, second dispenser being carried out after 7 days, each processes 4
Individual cell, each 20 square meter of cell.10 days investigation statisticses incidences before medicine and after second medicine, 5 points of each cell are random
Sampling, 5 plants of crops of per investigation, investigates the lesion area in whole strain per blade and accounts for the percentage rate of blade area and be classified, calculate
Disease index and prevention effect.
Expected preventive effect (%)=X+Y-XY/100 (wherein, X, Y are single dose preventive effect)
Grade scale：
0 grade：Disease-free spot；
1 grade：Leaf spot lesion is less than 5, and length is less than 1cm；
3 grades：Leaf spot lesion 6-10, part scab length is more than 1cm；
5 grades：Leaf spot lesion 11-25, part scab is linked to be piece, and lesion area accounts for the 10-217% of leaf area；
7 grades：Leaf spot lesion more than 26, scab are linked to be piece, and lesion area accounts for the 26-50% of leaf area；
9 grades：Scab is linked to be piece, and lesion area accounts for more than the 50% of leaf area or full leaf is withered.
The field control effectiveness test of embodiment and matched group to verticillium wilt.
| Process group | Dosage (a.i.g/hm2) | Prevention effect (%) |
| A：B(20:1) | 60 | 46.5 |
| A：B(17:1) | 30 | 84.2 |
| A：B(17:1) | 60 | 91.3 |
| A：B(15:1) | 60 | 90.1 |
| A：B(10:1) | 60 | 42.4 |
| A：B(5:1) | 60 | 47.3 |
| A：B(1:1) | 60 | 43.7 |
| A：B(1:5) | 60 | 42.8 |
| A：B(1:10) | 60 | 41.5 |
| A | 60 | 49.1 |
| B | 60 | 29.6 |
It can be seen that the proportioning collaboration under certain proportion of podophyllotoxin and avicularin to prevent and treat verticillium wilt effect apparent, it is and single
One podophyllotoxin has certain prevention effect, but effect is not notable, single avicularin prevention and control capability be also effect not
It is good.Podophyllotoxin is 15 with the ratio of the parts by weight of avicularin:1-17:When 1, synergy is most notable, and during other proportions
Effect is not very notable.
Claims (4)
1. a kind of bactericidal composition containing podophyllotoxin, it is characterised in that comprising two kinds of principle active components：Podophyllotoxin with it is flat
Store glycosides.
2. a kind of bactericidal composition containing podophyllotoxin according to claim 1, described podophyllotoxin and avicularin
The ratio of parts by weight is 1:1-20:1.
3. a kind of bactericidal composition containing podophyllotoxin according to claim 1, it is characterised in that it is characterized in that effectively
Composition accounts for the 1%～50% of gross weight.
4. a kind of bactericidal composition containing podophyllotoxin according to claim 1, it is characterised in that said composition can be made into
Suspending agent, wettable powder, seed coat agent, aqueous emulsion, microemulsion, cream, granule, water dispersible granules.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610969235.0A CN106561684A (en) | 2016-11-06 | 2016-11-06 | Bactericidal composition containing podophyllotoxin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610969235.0A CN106561684A (en) | 2016-11-06 | 2016-11-06 | Bactericidal composition containing podophyllotoxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106561684A true CN106561684A (en) | 2017-04-19 |
Family
ID=58541513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610969235.0A Pending CN106561684A (en) | 2016-11-06 | 2016-11-06 | Bactericidal composition containing podophyllotoxin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106561684A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115500355A (en) * | 2022-09-09 | 2022-12-23 | 广东省农业科学院果树研究所 | Prevention and treatment of peronophythora litchi by utilizing Podophyllotoxin and Gentisic acid |
-
2016
- 2016-11-06 CN CN201610969235.0A patent/CN106561684A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115500355A (en) * | 2022-09-09 | 2022-12-23 | 广东省农业科学院果树研究所 | Prevention and treatment of peronophythora litchi by utilizing Podophyllotoxin and Gentisic acid |
| CN115500355B (en) * | 2022-09-09 | 2024-02-09 | 广东省农业科学院果树研究所 | Control of downy mildew of litchi by Podophyllotoxin and Gentisic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103704215A (en) | Sterilization combination containing chitosan oligosaccharide | |
| CN103444729A (en) | Bactericidal pharmaceutical composition and applications thereof | |
| CN101589719B (en) | Bactericidal composition containing bromothalonil and prochloraz | |
| CN103329921A (en) | Benziothiazolinone-containing sterilization composite | |
| CN104106575B (en) | A kind of bactericidal composition containing pungent bacterium amine and olefin conversion | |
| CN106561684A (en) | Bactericidal composition containing podophyllotoxin | |
| CN101194620B (en) | Bactericide agent composition containing tebuconazole | |
| CN103329905B (en) | Sterilization composition containing penflufen and validamycin | |
| CN101595876B (en) | Bromothalonil and azoxystrobin containing sterilizing composition | |
| CN104886122B (en) | Compound composition containing dufulin and pyrazole amide bactericide and bactericide | |
| CN106561671A (en) | Tulathromycin-containing sterilization composition | |
| CN104770380A (en) | Tetramycin and difenoconazole containing sterilizing aqueous agent and preparation method thereof | |
| CN106577696A (en) | Tetramycin-containing pesticidal composition | |
| CN106417313A (en) | Bactericidal composition containing amisulbrom and fluopicolide | |
| CN105831132A (en) | Sterilization composition containing benzothiostrobin and fluoride ether bacteria amide | |
| CN104798793A (en) | Germicidal suspending agent containing tetramycin and difenoconazole and preparation method of germicidal suspending agent | |
| CN102396483B (en) | Antibacterial composition | |
| CN108669079A (en) | It is a kind of to be used to prevent Boscalid and eugenol compound pesticide preparation of eliminating vegetable botrytis and preparation method thereof | |
| CN106550942A (en) | A kind of bactericidal composition containing 1,8 cineoles | |
| CN106561652A (en) | Isosakuranetin containing sterilizing combination | |
| CN104585180B (en) | A kind of Pesticidal combination of containing pyriminostrobin | |
| CN106561674A (en) | Bactericidal composition containing isothiazolinone | |
| CN107980777A (en) | A kind of bactericidal composition containing bromothalonil and eugenol | |
| CN101595879B (en) | Bromothalonil and myclobutanil containing sterilizing composition | |
| CN107751210A (en) | A kind of bactericidal composition containing isopyrazam |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170419 |
|
| WD01 | Invention patent application deemed withdrawn after publication |