CN106560052A - Feed additive capable of improving disease resistance of livestock and poultry - Google Patents
Feed additive capable of improving disease resistance of livestock and poultry Download PDFInfo
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- CN106560052A CN106560052A CN201610981465.9A CN201610981465A CN106560052A CN 106560052 A CN106560052 A CN 106560052A CN 201610981465 A CN201610981465 A CN 201610981465A CN 106560052 A CN106560052 A CN 106560052A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Abstract
The invention discloses a feed additive capable of improving disease resistance of livestock and poultry. The feed additive is prepared from the following components by weight: 10 to 20 parts of freeze-dried powder of ginkgo leaf, 15 to 25 parts of olive leaf extract, 13 to 17 parts of wall-broken spirulina powder, 40 to 60 parts of honeysuckle flower extract chlorogenic acid, 15 to 25 parts of dipotassium glycyrrhizinate, 6 to 18 parts of Radix Astragali polysaccharide, 6 to 18 parts of Fructus Forsythiae extract, 6 to 18 parts of isatis root extract and 5 to 15 parts of a composite microbial agent. According to the invention, the olive leaf extract, 13 to 17 parts of wall-broken spirulina powder, the honeysuckle flower extract chlorogenic acid, the Fructus Forsythiae extract, the isatis root extract and the like are obtained through special process and combined with dipotassium glycyrrhizinate, Radix Astragali polysaccharide, the composite microbial agent and the like according to an optimal ratio, so the prepared feed additive has the medical health-care effects like bacteriostatic effect, antiviral effect and growth-promoting effect and does not incur generation of drug resistance.
Description
Technical field
The present invention relates to a kind of feed addictive, and in particular to a kind of feed addictive of raising livestock and poultry resistance against diseases.
Background technology
At present, increasing insider recognize antibiotic used in poultry and livestock feed, somatropin, preservative,
The conventional medicine such as antioxidant, disinfectant feed addictive is to the potential threat of human health.These traditional feed addictives
With various harm：One is to add antibiotic in feed for a long time, can induce the generation of resistance micropopulation in poultry body, by
In these microorganisms have antagonism antibiotic ability, once thus infection human body, patient will be made to past medical help, so as to people
Class causes greatly threat；Two is to add artificial synthesized somatropin in feed, loses poultry growth failure, product
Nutritive value originally and local flavor, quality decline, commodity value is reduced；Three be antibiotic, somatotropin, preservative, antioxidant,
The artificial synthesized chemicals such as disinfectant often has toxic and side effect, can cause the distortion of normal tissue cell, mutation, even
Cause canceration, be detrimental to health, the enrichment that these chemical substances pass through poultry is a large amount of in the liver and muscle of poultry
Ground is accumulated, and more exacerbates harmfulness；Additionally, these chemicalses there is likely to be, the not clear poison of some other mechanism is secondary to be made
With causing potential threat.Therefore, some developed countries propose one after another the proposal for prohibitting the use of antibiotic and Somatotropin, and
Relevant law bill is successively formulated, this proposal legalization is made.China's Chinese herbal medicine resource is developed, is replaced with Chinese herbal medicine often
The antibiotic of rule, somatotropin and chemicals additive are most promising developing direction.Chinese herb feed additive utilizes pure medicine
Be raw material with plant roots, stem, leaf and fruit, have no toxic side effect, in poultry body without hazard residue, meet ecological food and green food
The developing direction of product, caters to the needs of domestic and international market.
With Chinese herbal medicine, as feed addictive, to improve, the immunity of body is existing at home and abroad much to be reported.But due to mesh
Front research mostly lacks systematicness, often with reference to the formula for human body therapy, not with Chinese pharmacology, pharmacy, medicine
Principle of science also seldom considers the collaboration between each composition or antagonism targetedly studying livestock and poultry animal.2006 3
The moon 29, Chinese invention patent publication number CNI751587A discloses a kind of increase livestock and poultry resistance against diseases, reduces cholesterol blood fat
Feed addictive, be by 20 grams of the tuber of multiflower knotweed, 30 grams of the red sage root, 30 grams of cassia seed, 28 grams of giant knotweed, 50 grams of oriental wormwood, 40 grams of hawthorn, the root of kudzu vine
20 grams, 20 grams of dried orange peel, 25 grams of folium isatidis, 40 grams of ginkgo leaf, 30 grams of root tuber of aromatic turmeric, 50 grams of parasitic loranthus, 27 grams of perilla leaf, bluish dogbane 60
Gram, 40 grams of pseudo-ginseng, 40 grams of Asian puccoon, 45 grams of the Radix Astragali, 50 grams of Processing methods of honeysuckle crush, add in feed by 1.5%～2% and feed
Livestock and poultry., using Chinese medicine up to 18 kinds, the wherein valuable medicinal consumption such as the tuber of multiflower knotweed, the red sage root, pseudo-ginseng, parasitic loranthus is too high, compatibility for the formula
Science, action effect be not unstable, so both increased cost while again limiting feed formula space, sometimes can also be to feed
Palatability produce suppression.
The content of the invention
To solve the above problems, the invention provides a kind of feed addictive for improving livestock and poultry resistance against diseases, with suppression
The medical care effect such as bacterium, antiviral, growth promotion, does not develop immunity to drugs.
For achieving the above object, the technical scheme taken of the present invention is：
A kind of feed addictive for improving livestock and poultry resistance against diseases, is prepared from by the component of following weight portion：
10～20 parts of ginkgo leaf freeze-drying, 15～25 parts of Olive leaf P.E, 13～17 parts of broken wall spirulina powder, honeysuckle
40～60 parts of extract chlorogenic acid, 15～25 parts of dipotassium glycyrrhizinate, 6～18 parts of astragalus polyose, 6～18 parts of forsythia suspense extraction, plate
6～18 parts of blue root extract, 5～15 parts of composite bacteria agent.
Preferably, the Honegsukle flower P.E chlorogenic acid is as obtained by prepared by following steps：
S11, the aqueous solution of Honegsukle flower P.E is first passed through composite polrvinyl chloride milipore filter carry out UF membrane, collect and pass through
Liquid, then extract is concentrated into the 11% of original volume with the NF membrane of molecular cut off 250, collect concentrate；
S12, by the concentrate for obtaining with the flow velocity of 2～6BV/h by macroporous resin column, after Dynamic Adsorption saturation, spend
Ionized water is colourless to efflux with the above-mentioned macroporous resin column of flow velocity drip washing of 3～11BV/h, then with volume fraction be 30%～
110% organic solvent is eluted with the flow velocity of 7～13BV/h, collects organic solvent eluent until flowing out without chlorogenic acid,
Gained eluent reduced pressure concentration will be finally collected, medicinal extract will be obtained；
S13, will after medicinal extract water dissolves with the flow velocity of 1～5BV/h by polyamide column, after Dynamic Adsorption saturation, with 3～
8BV deionized waters with the above-mentioned polyamide column of flow velocity drip washing of 1～10BV/h, then with volume fraction be 20%～100% it is organic
Solvent aqueous solution is eluted with the flow velocity of 5～10BV/h, collects organic solvent eluent until flowing out without chlorogenic acid, and spraying is dry
It is dry, obtain chlorogenic acid.
Preferably, the forsythia suspense extraction is as obtained by prepared by following steps：
Dry Folium Forsythia is placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.7～1.3MPa, explosion
Process 8～25min；Then be passed through rapidly steam to steam explosion pressure inside the tank be 1.3～1.7MPa, Steam explosion treatment 0.5～
2.5min, filters, and the dregs of a decoction add water refluxing extraction 2 times in mass ratio for 1: 10, and each refluxing extraction 1 hour merges 3 times and extracts
Liquid, is concentrated into relative density 1.10～1.20 (room temperature), is spray-dried concentrate, obtains Folium Forsythiae extract；
Preferably, the isatis root extract is as obtained by prepared by following steps：
The flash extracter process of dry Radix Isatidis, gained extract are first heated with high heat, added with slow fire after boiling
1～1.5h of heat, hollow fiber ultrafiltration membrane is filtered；The dregs of a decoction add 15～and 25 times of decoctings boil twice, and hollow fiber ultrafiltration membrane is filtered, and is merged
Three filtrates, are concentrated into 1.5g/ml；90～100% organic solvents are slowly added to, until organic solvent concentration is 55～65%,
Refrigeration 8～9 hours, 2000～4000r/min is centrifuged after 15～25min and goes supernatant, precipitate with deionized water to be dissolved to original
Volume, with organic solvent deposit 2 times, organic solvent concentration is respectively 65～75%, 75～85%；Precipitate with deionized water dissolves
To original volume, the solution of trichloroacetic acid of 1/6～1/5 volume 10～12% is added, 4～6h of standing after fully mixing, 2000～
4000r/min is centrifuged after 15～25min and goes to precipitate to obtain supernatant, by gained supernatant suction filtration, drying, obtains isatis root extract.
Preferably, the ginkgo leaf freeze-drying is as obtained by prepared by following steps：
Take clean drain after ginkgo leaf be placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.7～
1.5MPa, 7～23min of explosion treatment；Then be passed through rapidly steam to steam explosion pressure inside the tank be 1.5～2.1MPa, steam blasting
After processing 0.8～2.8min, according to the ratio microbe inoculation leavening of inoculum concentration 1.5～1.7%, keeping temperature 25～35
DEG C, after fermenting 48～54 hours, high-temperature sterilization, freeze-drying obtains ginkgo leaf freeze-drying.
Preferably, the Olive leaf P.E is as obtained by prepared by following steps：
S1, the olive leaf that drains will be cleaned be placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.6～
1.4MPa, 8～25min of explosion treatment；Then be passed through rapidly steam to steam explosion pressure inside the tank be 1.4～1.8MPa, steam blasting
After processing 0.5～2.5min, room temperature is concentrated into relative density 1.10～1.20, is spray-dried concentrate, obtains powder；
S2, in powder add 3～8 times of water, by mass percentage for 2～3% ratio add neutral proteinase, by matter
Amount percentage is 1～2% addition flavor protease, and 1～2h is digested at 30～50 DEG C, is filtered, and obtains enzymolysis liquid；
S3, by naturally to thaw again after the zymotic fluid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant
Liquid, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3～7BV/h by macroporous resin column, after Dynamic Adsorption saturation, deionized water with
The above-mentioned macroporous resin column of flow velocity drip washing of 5～8BV/h is colourless to efflux, then with volume fraction be 50%～70% it is organic molten
Agent is eluted with the flow velocity of 8～12BV/h, collects eluent, and reduced pressure concentration obtains Olive leaf P.E.
Preferably, the broken wall spirulina powder is as obtained by prepared by following steps：
Spirulina after cleaning is placed in after soaking 10 days in distilled water, pulls out and be placed in low salt solutions immersion 3～4 days, so
After pull out drain after be placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.8～1.4MPa, explosion treatment 8～
25min；Then it is 1.4～1.8MPa to be passed through steam rapidly to steam explosion pressure inside the tank, after 0.5～2.5min of Steam explosion treatment,
It is spray-dried to obtain broken wall spirulina powder.
Preferably, the composite polrvinyl chloride milipore filter is prepared from by the raw material of following masses number：
40～65 parts of polyvinyl chloride, 20～40 parts of polysulfones, 45～65 parts of dimethyl sulfoxide (DMSO), 15～25 parts of acetate fiber, nothing
3～15 parts of water lithium chloride.
Preferably, the molecular cut off of the hollow fiber ultrafiltration membrane is 1000.
Preferably, the composite bacteria agent is by Candida zymotic fluid dried object and Kluyveromyces fragilis zymotic fluid dried object
By the mixing gained of quality 2: 5.
The invention has the advantages that：
By special technique, Olive leaf P.E, Honegsukle flower P.E chlorogenic acid, Folium Forsythiae extract and plate are obtained
Blue root extract etc., and they are combined together with dipotassium glycyrrhizinate, astragalus polyose, composite bacteria agent etc. by optimum proportioning,
The medical care effect such as antibacterial, antiviral, growth promotion, and do not develop immunity to drugs.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, the present invention is carried out further with reference to embodiments
Describe in detail.It should be appreciated that specific embodiment described herein is not used to limit this only to explain the present invention
It is bright.
In following examples, the organic solvent for being used is the turpentine derivatives of natural origin, and the turpentine oil derives
Thing is isomery, the disproportionation products of firpene.
The Honegsukle flower P.E chlorogenic acid for being used is as obtained by prepared by following steps：
S11, the aqueous solution of Honegsukle flower P.E is first passed through composite polrvinyl chloride milipore filter carry out UF membrane, collect and pass through
Liquid, then extract is concentrated into the 11% of original volume with the NF membrane of molecular cut off 250, collect concentrate；
S12, by the concentrate for obtaining with the flow velocity of 2～6BV/h by macroporous resin column, after Dynamic Adsorption saturation, spend
Ionized water is colourless to efflux with the above-mentioned macroporous resin column of flow velocity drip washing of 3～11BV/h, then with volume fraction be 30%～
110% organic solvent is eluted with the flow velocity of 7～13BV/h, collects organic solvent eluent until flowing out without chlorogenic acid,
Gained eluent reduced pressure concentration will be finally collected, medicinal extract will be obtained；
S13, will after medicinal extract water dissolves with the flow velocity of 1～5BV/h by polyamide column, after Dynamic Adsorption saturation, with 3～
8BV deionized waters with the above-mentioned polyamide column of flow velocity drip washing of 1～10BV/h, then with volume fraction be 20%～100% it is organic
Solvent aqueous solution is eluted with the flow velocity of 5～10BV/h, collects organic solvent eluent until flowing out without chlorogenic acid, and spraying is dry
It is dry, obtain chlorogenic acid.
The forsythia suspense extraction for being used is as obtained by prepared by following steps：
Dry Folium Forsythia is placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.7～1.3MPa, explosion
Process 8～25min；Then be passed through rapidly steam to steam explosion pressure inside the tank be 1.3～1.7MPa, Steam explosion treatment 0.5～
2.5min, filters, and the dregs of a decoction add water refluxing extraction 2 times in mass ratio for 1: 10, and each refluxing extraction 1 hour merges 3 times and extracts
Liquid, is concentrated into relative density 1.10～1.20 (room temperature), is spray-dried concentrate, obtains Folium Forsythiae extract；
The isatis root extract for being used is as obtained by prepared by following steps：
The flash extracter process of dry Radix Isatidis, gained extract are first heated with high heat, added with slow fire after boiling
1～1.5h of heat, hollow fiber ultrafiltration membrane is filtered；The dregs of a decoction add 15～and 25 times of decoctings boil twice, and hollow fiber ultrafiltration membrane is filtered, and is merged
Three filtrates, are concentrated into 1.5g/ml；90～100% organic solvents are slowly added to, until organic solvent concentration is 55～65%,
Refrigeration 8～9 hours, 2000～4000r/min is centrifuged after 15～25min and goes supernatant, precipitate with deionized water to be dissolved to original
Volume, with organic solvent deposit 2 times, organic solvent concentration is respectively 65～75%, 75～85%；Precipitate with deionized water dissolves
To original volume, the solution of trichloroacetic acid of 1/6～1/5 volume 10～12% is added, 4～6h of standing after fully mixing, 2000～
4000r/min is centrifuged after 15～25min and goes to precipitate to obtain supernatant, by gained supernatant suction filtration, drying, obtains isatis root extract.
The ginkgo leaf freeze-drying for being used is as obtained by prepared by following steps：
Take clean drain after ginkgo leaf be placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.7～
1.5MPa, 7～23min of explosion treatment；Then be passed through rapidly steam to steam explosion pressure inside the tank be 1.5～2.1MPa, steam blasting
After processing 0.8～2.8min, according to the ratio microbe inoculation leavening of inoculum concentration 1.5～1.7%, keeping temperature 25～35
DEG C, after fermenting 48～54 hours, high-temperature sterilization, freeze-drying obtains ginkgo leaf freeze-drying.
The Olive leaf P.E for being used is as obtained by prepared by following steps：
S1, the olive leaf that drains will be cleaned be placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.6～
1.4MPa, 8～25min of explosion treatment；Then be passed through rapidly steam to steam explosion pressure inside the tank be 1.4～1.8MPa, steam blasting
After processing 0.5～2.5min, room temperature is concentrated into relative density 1.10～1.20, is spray-dried concentrate, obtains powder；
S2, in powder add 3～8 times of water, by mass percentage for 2～3% ratio add neutral proteinase, by matter
Amount percentage is 1～2% addition flavor protease, and 1～2h is digested at 30～50 DEG C, is filtered, and obtains enzymolysis liquid；
S3, by naturally to thaw again after the zymotic fluid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant
Liquid, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3～7BV/h by macroporous resin column, after Dynamic Adsorption saturation, deionized water with
The above-mentioned macroporous resin column of flow velocity drip washing of 5～8BV/h is colourless to efflux, then with volume fraction be 50%～70% it is organic molten
Agent is eluted with the flow velocity of 8～12BV/h, collects eluent, and reduced pressure concentration obtains Olive leaf P.E.
The broken wall spirulina powder for being used is as obtained by prepared by following steps：
Spirulina after cleaning is placed in after soaking 10 days in distilled water, pulls out and be placed in low salt solutions immersion 3～4 days, so
After pull out drain after be placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.8～1.4MPa, explosion treatment 8～
25min；Then it is 1.4～1.8MPa to be passed through steam rapidly to steam explosion pressure inside the tank, after 0.5～2.5min of Steam explosion treatment,
It is spray-dried to obtain broken wall spirulina powder.
The composite polrvinyl chloride milipore filter for being used is prepared from by the raw material of following masses number：
40～65 parts of polyvinyl chloride, 20～40 parts of polysulfones, 45～65 parts of dimethyl sulfoxide (DMSO), 15～25 parts of acetate fiber, nothing
3～15 parts of water lithium chloride.
The molecular cut off of the hollow fiber ultrafiltration membrane for being used is 1000.
The composite bacteria agent for being used is pressed by Candida zymotic fluid dried object and Kluyveromyces fragilis zymotic fluid dried object
The mixing gained of quality 2: 5
Embodiment 1
A kind of feed addictive for improving livestock and poultry resistance against diseases, is prepared from by the component of following weight portion：
10 parts of ginkgo leaf freeze-drying, 15 parts of Olive leaf P.E, 13 parts of broken wall spirulina powder, Honegsukle flower P.E chlorogenic acid
40 parts, 15 parts of dipotassium glycyrrhizinate, 6 parts of astragalus polyose, 6 parts of forsythia suspense extraction, 6 parts of isatis root extract, 5 parts of composite bacteria agent.
Embodiment 2
A kind of feed addictive for improving livestock and poultry resistance against diseases, is prepared from by the component of following weight portion：
20 parts of ginkgo leaf freeze-drying, 25 parts of Olive leaf P.E, 17 parts of broken wall spirulina powder, Honegsukle flower P.E chlorogenic acid
60 parts, 25 parts of dipotassium glycyrrhizinate, 18 parts of astragalus polyose, 18 parts of forsythia suspense extraction, 18 parts of isatis root extract, composite bacteria agent 15
Part.
Embodiment 3
A kind of feed addictive for improving livestock and poultry resistance against diseases, is prepared from by the component of following weight portion：
15 parts of ginkgo leaf freeze-drying, 20 parts of Olive leaf P.E, 15 parts of broken wall spirulina powder, Honegsukle flower P.E chlorogenic acid
50 parts, 20 parts of dipotassium glycyrrhizinate, 12 parts of astragalus polyose, 12 parts of forsythia suspense extraction, 12 parts of isatis root extract, composite bacteria agent 10
Part.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. it is a kind of improve livestock and poultry resistance against diseases feed addictive, it is characterised in that be prepared from by the component of following weight portion：
10～20 parts of ginkgo leaf freeze-drying, 15～25 parts of Olive leaf P.E, 13～17 parts of broken wall spirulina powder, honeysuckle are extracted
40～60 parts of thing chlorogenic acid, 15～25 parts of dipotassium glycyrrhizinate, 6～18 parts of astragalus polyose, 6～18 parts of forsythia suspense extraction, Radix Isatidis
6～18 parts of extract, 5～15 parts of composite bacteria agent.
2. a kind of feed addictive for improving livestock and poultry resistance against diseases as claimed in claim 1, it is characterised in that the honeysuckle
Extract chlorogenic acid is as obtained by prepared by following steps：
S11, the aqueous solution of Honegsukle flower P.E first passed through composite polrvinyl chloride milipore filter carry out UF membrane, collect permeate,
Again extract is concentrated into the 11% of original volume with the NF membrane of molecular cut off 250, collects concentrate；
S12, by the concentrate for obtaining with the flow velocity of 2～6BV/h by macroporous resin column, after Dynamic Adsorption saturation, use deionization
Water is colourless to efflux with the above-mentioned macroporous resin column of flow velocity drip washing of 3～11BV/h, then is 30%～110% with volume fraction
Organic solvent is eluted with the flow velocity of 7～13BV/h, collects organic solvent eluent until flowing out without chlorogenic acid, finally will be received
Collection gained eluent reduced pressure concentration, obtains medicinal extract；
S13, will after medicinal extract water dissolves with the flow velocity of 1～5BV/h by polyamide column, after Dynamic Adsorption saturation, with 3～8BV
Deionized water with the above-mentioned polyamide column of flow velocity drip washing of 1～10BV/h, then with the organic solvent that volume fraction is 20%～100%
The aqueous solution is eluted with the flow velocity of 5～10BV/h, collects organic solvent eluent until flowing out without chlorogenic acid, is spray-dried,
Obtain chlorogenic acid.
3. a kind of feed addictive for improving livestock and poultry resistance against diseases as claimed in claim 1, it is characterised in that the capsule of weeping forsythia is carried
Take thing as following steps prepare obtained by：
Dry Folium Forsythia is placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.7～1.3MPa, explosion treatment
8～25min；Then be passed through rapidly steam to steam explosion pressure inside the tank be 1.3～1.7MPa, 0.5～2.5min of Steam explosion treatment,
Filter, the dregs of a decoction add water refluxing extraction 2 times in mass ratio for 1: 10, and each refluxing extraction 1 hour, No. 3 extracts of merging are concentrated into
Relative density 1.10～1.20 (room temperature), is spray-dried concentrate, obtains Folium Forsythiae extract.
4. a kind of feed addictive for improving livestock and poultry resistance against diseases as claimed in claim 1, it is characterised in that the Radix Isatidis
Extract is as obtained by prepared by following steps：
The flash extracter process of dry Radix Isatidis, gained extract are first heated with high heat, after boiling with slow fire heating 1～
1.5h, hollow fiber ultrafiltration membrane is filtered；The dregs of a decoction add 15～and 25 times of decoctings boil twice, and hollow fiber ultrafiltration membrane is filtered, and is merged three times
Filtrate, is concentrated into 1.5g/ml；90～100% organic solvents are slowly added to, until organic solvent concentration is 55～65%, refrigeration 8
～9 hours, 2000～4000r/min was centrifuged after 15～25min and goes supernatant, precipitate with deionized water to be dissolved to original volume,
With organic solvent deposit 2 times, organic solvent concentration is respectively 65～75%, 75～85%；Precipitate with deionized water is dissolved to original
Volume, adds the solution of trichloroacetic acid of 1/6～1/5 volume 10～12%, and 4～6h, 2000～4000r/ are stood after fully mixing
Min is centrifuged after 15～25min and goes to precipitate to obtain supernatant, by gained supernatant suction filtration, drying, obtains isatis root extract.
5. a kind of feed addictive for improving livestock and poultry resistance against diseases as claimed in claim 1, it is characterised in that the ginkgo leaf
Freeze-dried powder is as obtained by prepared by following steps：
Take the ginkgo leaf cleaned after draining to be placed in steam-explosion jar, it is 0.7～1.5MPa to be first passed through nitrogen to steam explosion pressure inside the tank, quick-fried
Tear manages 7～23min；Then be passed through rapidly steam to steam explosion pressure inside the tank be 1.5～2.1MPa, Steam explosion treatment 0.8～
After 2.8min, according to the ratio microbe inoculation leavening of inoculum concentration 1.5～1.7%, 25～35 DEG C of keeping temperature, fermentation 48～
After 54 hours, high-temperature sterilization, freeze-drying obtains ginkgo leaf freeze-drying.
6. a kind of feed addictive for improving livestock and poultry resistance against diseases as claimed in claim 1, it is characterised in that the olive leaf
Extract is as obtained by prepared by following steps：
S1, the olive leaf that drains will be cleaned it is placed in steam-explosion jar, it is 0.6～1.4MPa to be first passed through nitrogen to steam explosion pressure inside the tank,
8～25min of explosion treatment；Then be passed through rapidly steam to steam explosion pressure inside the tank be 1.4～1.8MPa, Steam explosion treatment 0.5
After～2.5min, room temperature is concentrated into relative density 1.10～1.20, is spray-dried concentrate, obtains powder；
S2, in powder add 3～8 times of water, by mass percentage for 2～3% ratio add neutral proteinase, by quality hundred
Divide than adding flavor protease for 1～2%, 1～2h is digested at 30～50 DEG C, filtration obtains enzymolysis liquid；
S3, by naturally to thaw again after the zymotic fluid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant,
Remainder is centrifuged, and collects centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3～7BV/h by macroporous resin column, after Dynamic Adsorption saturation, deionized water with 5～
The above-mentioned macroporous resin column of flow velocity drip washing of 8BV/h is colourless to efflux, then with the organic solvent that volume fraction is 50%～70%
Eluted with the flow velocity of 8～12BV/h, collected eluent, reduced pressure concentration is obtained Olive leaf P.E.
7. a kind of feed addictive for improving livestock and poultry resistance against diseases as claimed in claim 1, it is characterised in that the broken wall spiral shell
Spirulina powder is as obtained by prepared by following steps：
Spirulina after cleaning is placed in after soaking 10 days in distilled water, pulls out and be placed in low salt solutions immersion 3～4 days, then dragged for
Go out after draining and be placed in steam-explosion jar, it is 0.8～1.4MPa, 8～25min of explosion treatment to be first passed through nitrogen to steam explosion pressure inside the tank；
Then it is 1.4～1.8MPa to be passed through steam rapidly to steam explosion pressure inside the tank, and after 0.5～2.5min of Steam explosion treatment, spraying is dry
It is dry to obtain broken wall spirulina powder.
8. a kind of feed addictive for improving livestock and poultry resistance against diseases as claimed in claim 1, it is characterised in that described compound poly-
Vinyl chloride milipore filter is prepared from by the raw material of following masses number：
40～65 parts of polyvinyl chloride, 20～40 parts of polysulfones, 45～65 parts of dimethyl sulfoxide (DMSO), 15～25 parts of acetate fiber, anhydrous chlorine
Change 3～15 parts of lithium.
9. a kind of feed addictive for improving livestock and poultry resistance against diseases as claimed in claim 1, it is characterised in that the hollow fibre
The molecular cut off of dimension milipore filter is 1000.
10. a kind of feed addictive for improving livestock and poultry resistance against diseases as claimed in claim 1, it is characterised in that described compound
Microbial inoculum is by Candida zymotic fluid dried object and Kluyveromyces fragilis zymotic fluid dried object by the mixing gained of quality 2: 5.
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| CN107308268A (en) * | 2017-07-04 | 2017-11-03 | 信阳农林学院 | A kind of composition for treating sheep infective warts |
| CN108056065A (en) * | 2017-12-19 | 2018-05-22 | 陈永魁 | A kind of field cultural method of Gallus domesticlus brisson |
| CN109043143A (en) * | 2018-11-07 | 2018-12-21 | 辽宁职业学院 | A kind of selenium-rich edible fungi residue feed and its application |
| CN111494453A (en) * | 2020-05-18 | 2020-08-07 | 南京大方生物工程有限公司 | Preparation method and application of compound natural plant extract with heat-clearing and detoxifying effects |
| CN114984042A (en) * | 2022-07-14 | 2022-09-02 | 江西高胜动物保健品有限公司 | Compound traditional Chinese medicine extract and iodine compounded solution preparation and preparation method thereof |
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Application publication date: 20170412 |