CN106554428A

CN106554428A - A kind of method that chondroitin sulfate is extracted in fish processing fent

Info

Publication number: CN106554428A
Application number: CN201510634545.2A
Authority: CN
Inventors: 霍波
Original assignee: GUANGXI ZHENGWU MARINE INDUSTRY Co Ltd
Current assignee: GUANGXI ZHENGWU MARINE INDUSTRY Co Ltd
Priority date: 2015-09-30
Filing date: 2015-09-30
Publication date: 2017-04-05

Abstract

The invention discloses a kind of method that chondroitin sulfate is extracted in fish processing fent, mainly comprises the steps：Raw material is cleaned, is boiled in being put into 90 DEG C of water 1～2 hour, filtration crush net fishbone powder is standby；Net fishbone powder presses 1 with 1mol/L sodium hydroxide solutions:The weight ratio of 1-1.5 adds sodium hydroxide solution, and 4h is extracted in stirring at 45 DEG C, adjusts extracting solution ph for 8-10, and double-layer filter cloth filters to get filtrate, and repeats to extract once by above-mentioned condition, and filtrate merges standby twice；According to filtrate and compound protease 1000:Compound protease of 1 weight than addition activity ten thousand U/g of 100-200, temperature 40-50 DEG C hydrolysis 4h obtain hydrolyzed solution, 80 DEG C of heating 30min, ph to 2-3 is adjusted, solution layering, upper solution Pumex soil sucking filtration is stood, filtrate adjusts ph7, control filtrate temperature is concentrated in vacuo to original volume 1/4 at 50-60 DEG C, adds 2-3 times of volume dehydrated alcohol precipitation, stand 6h, filter, filtrate repeats to precipitate with 75% ethanol, collection precipitate under temperature 60 C is dried 4h and obtains final product.

Description

A kind of method that chondroitin sulfate is extracted in fish processing fent

Technical field

The present invention relates to a kind of method that chondroitin sulfate is extracted in fish processing fent, belongs to marine fishery processing technique field.

Background technology

Chondroitin sulfate is to be covalently attached the class glycosaminoglycans that Dan Baiduotang proteoglycan PG is formed on protein, the polysaccharide chain that its molecular structure is consisted of the repetition disaccharide unit of 1 → 3 glucosides bond formeds of β β-D- glucuronic acids and N- acetylamino galactosamines.Chondroitin sulfate is generally combined into protein in the form of glycoprotein, and elimination reaction occurs when which hydrolyzes, and is connected the glycosidic bonds of chondroitin sulfate and serine hydroxyl in protein, can be obtained the chondroitin sulfate of free state.During chondroitin sulfate is primarily present in animal connective tissue and in fishbone, the higher chondroitin sulfate of content be there is in the leftover bits and pieces after fish processing, fish processing fent includes fish head, fishbone and fish scale, therefore, it is the beneficial pathways for improving the side-product added value after fish is processed to extract chondroitin sulfate in processing fent.The at present extraction of chondroitin sulfate adopts the methods such as alkaline extraction, ultrasonic disruption, membrance separation mostly, but all there are such as complex steps, and long the production cycle, high cost, yield are low, the problems such as quality is unstable.

The content of the invention

A kind of the technical problem to be solved extracts chondroitin sulfate method in being to provide fish processing fent, solves traditional extraction process complex steps, and long the production cycle, high cost, yield are low, the unstable problem of quality.

The solution of above-mentioned technical problem is to employ following technical measures, a kind of method that chondroitin sulfate is extracted in fish processing fent, is mainly comprised the steps：

First, fishbone, fish head and fish scale are cleaned, is boiled in being put into 90 DEG C of water 1～2 hour, filtered, taken net fishbone, fish head and fish scale and crush, obtain net fishbone powder standby；

2nd, net fishbone powder and 1mol/L sodium hydroxide solutions are according to 1:The weight ratio of 1-1.5 adds sodium hydroxide solution, and 4h is extracted in stirring at 45 DEG C, then adjusts extracting solution ph for 8-10, and double-layer filter cloth is filtered, and obtains filtrate, repeats to extract once by above-mentioned condition, and filtrate merges twice, standby；

3rd, according to filtrate and compound protease 1000:1 weight obtains hydrolyzed solution, hydrolyzed solution is heated to 80 DEG C of 30min than the compound protease that addition activity is ten thousand U/g of 100-200, keeping temperature 40-50 DEG C hydrolysis 4h, ph to 2-3 is adjusted, solution layering, upper solution Pumex soil sucking filtration is stood, filtrate adjusts ph7, by filtrate temperature control at 50-60 DEG C, is concentrated in vacuo to original volume 1/4, add 2-3 times of volume dehydrated alcohol precipitation, 6 little, filtrations are stood, filtrate is precipitated with the repetition of 75% ethanol, collect precipitate and 4h is dried under temperature 60 C, obtain final product.

The present invention for taking above-mentioned measure, is prepared using enzymatic isolation method, and is concentrated through multiple times of filtration, and obtained chondroitin sulfate compares traditional method step mild condition, and energy consumption is low, and yield is higher, and product purity is high, steady quality reliability.

Specific embodiment

Embodiment 1

First, take shark bone 10kg to clean, boil 1 hour as 90 hot water, take out net shark bone and be ground into the bone flour that particle diameter is 0.1-0.3 millimeters；

2nd, in bone flour the sodium hydroxide solution 15kg that concentration is 1mol/L, stirring at 45 DEG C is added to extract 4h, it is 8 then to adjust extracting solution ph with acid, and double-layer filter cloth is filtered, and filtrate is stand-by；

Three, the compound protease that 0.015kg activity is 1,000,000 U/gt is added in filtrate, 50 DEG C of hydrolysis 4h of keeping temperature obtain hydrolyzed solution, hydrolyzed solution is heated to into 80 DEG C of 30min, hydrolyzed solution ph to 3 is adjusted with acid, stand solution layering, upper solution Pumex soil sucking filtration, filtrate adjusts ph7, by filtrate temperature control at 60 DEG C, it is concentrated in vacuo to original volume 1/4, add the dehydrated alcohol of 3 times of amount volumes, stand 6 hours, obtain crystalline deposit, filter, filtrate adds 75% ethanol of 2 times of amount volumes, stand 6 hours, obtain crystalline deposit, collect crystalline deposit thing twice, 4h is dried under temperature 60 C, obtain final product.

Embodiment 2

First, take trash fish bone and fish scale 100kg is cleaned, boil 2 hours as 90 hot water, take out net trash fish bone meal and be broken into the bone flour that particle diameter is 0.1-0.3 millimeters；

2nd, in bone flour the sodium hydroxide solution that 100kg concentration is 1mol/L, stirring at 45 DEG C is added to extract 4h, it is 10 then to adjust ph, and double-layer filter cloth is filtered, and filtrate is stand-by；

3rd, in filtrate, addition 0.1kg activity is the compound protease of 2,000,000 U/g, 40 DEG C of hydrolysis 4h of keeping temperature, hydrolyzed solution is heated to into 80 DEG C of 30min, ph to 2 is adjusted with acid, stand solution layering, upper solution Pumex soil sucking filtration, filtrate adjusts ph7, by filtrate temperature control at 50 DEG C, is concentrated in vacuo to original volume 1/4, add 2 times of amount volume dehydrated alcohol, 6 hours are stood, is filtered, is obtained crystalline deposit, filtrate adds 75% ethanol, 6 hours are stood, crystalline deposit is obtained, precipitate is collected, 4h is dried under temperature 60 C, is obtained final product.

Claims

1. a kind of method that chondroitin sulfate is extracted in fish processing fent, it is characterised in that methods described mainly comprises the steps：

（1）, fishbone, fish head and fish scale cleaned, boil in being put into 90 DEG C of water 1～2 hour, filter, take net fishbone, fish head and fish scale and crush, obtain net fishbone powder standby；

（2）, net fishbone powder and 1mol/L sodium hydroxide solutions be according to 1:The weight ratio of 1-1.5 adds sodium hydroxide solution, and 4h is extracted in stirring at 45 DEG C, then adjusts extracting solution ph for 8-10, and double-layer filter cloth is filtered, and obtains filtrate, repeats to extract once by above-mentioned condition, and filtrate merges twice, standby；

（3）, according to filtrate and compound protease 1000:1 weight obtains hydrolyzed solution, hydrolyzed solution is heated to 80 DEG C of 30min than the compound protease that addition activity is ten thousand U/g of 100-200, keeping temperature 40-50 DEG C hydrolysis 4h, ph to 2-3 is adjusted, solution layering, upper solution Pumex soil sucking filtration is stood, filtrate adjusts ph7, by filtrate temperature control at 50-60 DEG C, is concentrated in vacuo to original volume 1/4, add 2-3 times of volume dehydrated alcohol precipitation, 6 little, filtrations are stood, filtrate is precipitated with the repetition of 75% ethanol, collect precipitate and 4h is dried under temperature 60 C, obtain final product.