CN106538183A - A kind of method of Desert Control - Google Patents
A kind of method of Desert Control Download PDFInfo
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- CN106538183A CN106538183A CN201610912746.9A CN201610912746A CN106538183A CN 106538183 A CN106538183 A CN 106538183A CN 201610912746 A CN201610912746 A CN 201610912746A CN 106538183 A CN106538183 A CN 106538183A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0293—Seed or shoot receptacles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/021—Pots formed in one piece; Materials used therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to a kind of method of Desert Control, comprises the steps:Load non-woven fabrics after biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation You machine Fei Liao ﹑ plant seeds are proportionally mixed, the flat band that fixes the sand is seamed into sewing machine, the band that fixes the sand surrounds maximal side and is arranged on pending desert section on three sides of 0.5 2m or quadrilateral mesh continuous arrangement, arranges fixing device according to desert area wind-force and fixes the sand band;The suitable plant seed germination of band humidity that fixes the sand, holding is made to fix the sand band humidity in suitable seed germination and growth scope by means of natural or artificial rainwater;The band composition grid cell that fixes the sand becomes the ultimate unit that fixes the sand.Non-woven fabrics are designed to preferably Xu Shui ﹑ moisturizings;And biological compound fertilizer ﹑ composite fermentations have machine fertilizer material ﹑ intensive treatment mushroom bacteria rod that the nutritional labeling of abundance can be provided for plant seed;Control of desert efficiency can be effectively improved using the inventive method, afforestation effect, later stage desert afforestation good stability is improved.
Description
Technical field
The present invention relates to a kind of method of Desert Control.
Background technology
Generally investigated according to national desert, Gobi desert and sandy land and desertification finding shows, DESERTIFICATION IN CHINA soil face
Product is 262.2 ten thousand square kilometres, accounts for the 27.4% of area, and nearly 400,000,000 population is affected by desertification.According to China, U.S.
State, Canadian international cooperative project research show that China is about 54,100,000,000 RMB because of the direct economic loss that desertification is caused.
It is in DESERTIFICATION IN CHINA soil, maximum with the blow desertification area that strong wind is caused, account for 160.7 ten thousand square kilometres.According to statistics, 70
Since age, only desertification of land area expands speed, just has 2460 square kilometres every year.
A kind of lengths are disclosed in patent 200720084211.3, it is permeable mesh flake that what which was done is a kind of one side,
It is simultaneously the sack of waterproof diaphragm.Primarily to solving the problems, such as water-retention moisturizing, it is ensured that the abundance water of seedling.But
It is that water is all existed this technology the bottom of film, causes moisture distribution uneven, be also unfavorable for that seedling takes root, and does not reach what is fixed the sand
Effect, therefore effect is unsatisfactory.
A kind of sand-fixing greening net is disclosed in patent 02287068.7, is made up of numb straw rope, buckle and grass seed fixation group.Fiber crops
After straw rope is drawn as network structure, intersection is fastened with buckle, and grass seed fixed column is bonded on buckle.This sand-fixing greening net is just
In mechanized operation, and the careless lattice being paved into serve the effect for preventing sand dune movement, and grass seed used is fixed post design and is also beneficial to
Germination grows.But, the sand-fixing greening net usable area involved by the utility model patent is less, i.e. the long Seedling of institute's energy, rises
The grass seed fixed column area of Greening functions is less, and during laying large area, utilization rate is low, is unfavorable for that large area greening is adopted.
A kind of compound microbial culture starter, application number:201310744013.5, the present invention discloses aspergillus niger
(Aspergillus niger) Li-2013-03 preserving numbers are CGMCCNO.7927.
How effectively to realize that control of desert and immobilization are the new problems that a need are constantly explored.
The content of the invention:
The present invention is in order to overcome the defect of above-mentioned prior art, there is provided a kind of green coverage great ﹑ emergence rate Gao ﹑ can have
The method of the Desert Control of effect ground Gu Sha ﹑ Xu Shui ﹑ moisturizings, comprises the steps:
By the biological compound fertilizer for preparing, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed according to than
Load the band that fixes the sand of non-woven fabrics sack after example mixing, the band that fixes the sand surrounds maximal side in three sides of 0.5-2m or network of quadrilaterals
Lattice continuous arrangement is arranged on pending desert section, arranges fixing device according to desert area wind-force and fixes the sand band.By means of
Natural or artificial rainwater makes the suitable plant seed germination of band humidity that fixes the sand, holding fix the sand band humidity in suitable seed germination and growth
Scope.The band composition grid cell that fixes the sand becomes the ultimate unit that fixes the sand.
The parts by weight of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
Composition is as follows:Biological compound fertilizer 2-5 parts, intensive treatment mushroom bacteria rod 5-10 parts, composite fermentation organic fertilizer 15-30 parts, plant
Seed 0.2-1 parts.
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:Yeast bacteria agent
20-35 parts, maize cob meal 3-6 parts, aspergillus niger culture 8-15 part, N-hydroxy-succinamide 1-2 parts.
The yeast bacteria agent is commercially available active dry yeast or other yeast agents.
The preparation method of the intensive treatment mushroom bacteria rod is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition egg
Shell powder, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid, maintain the temperature at 20-30 DEG C after turning uniformly, cultivate
Add after 8-15 hours after aspergillus niger culture mixes at 25-30 DEG C, after culture 18-40 hours, it is equal that keeping temperature turns mixing
Even, 10 DEG C of placements are stand-by.
The weight portion of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Array is into as follows:Crab flavour mushroom bacterium rod 8-15 parts, egg-shell meal 0.5-1 parts, powder of straw 3-8 part, crushing maize core powder 5-10 parts, fermentation
Vinegar 2-5 parts, yeast liquid 1-3 part.
2-5% of the addition of the aspergillus niger culture for crab flavour mushroom bacterium rod quality.
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation
Proportionally mix with cattle manure, addition compound bacteria adjusts moisture and builds heap fermentation, temperature is turned when reaching 65 DEG C once, continue to send out
Ferment, does not turn in sweat, and 4-10 hours, addition cyclodextrin, shitosan, γ-poly- paddy are kept after temperature reaches 70-80 DEG C
Propylhomoserin continues fermentation, and holding is turned at intervals of every 10-20 hours once, treats that temperature reaches 25-40 DEG C of end fermentation process.
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Corn cob
Powder 4-8 parts, crushing straw 2-4 parts, Gypsum Fibrosum powder 0.5-2 parts, the useless vinegar 2-5 parts of fermentation, cattle manure 10-20 parts.
The parts by weight composition of the compound bacteria:Yeast liquid 1-2 part, aspergillus niger culture 4-8 part.
1-2% of the addition of the compound bacteria for maize cob meal quality.
1-3% of the cyclodextrin addition for maize cob meal quality.
1-2% of the shitosan addition for maize cob meal quality.
0.1-0.5% of the gamma-polyglutamic acid-addition for maize cob meal quality.
The cultural method of the yeast liquid comprises the steps:Using common saccharomycete seed liquor body cultural method:Will
It is stored in the yeast seeds in sand pipe, lyophilization pipe in a dormant state and accesses test tube slant activation, inoculation liquid training
Foster base culture 15-20 hours or continuation switching culture all may be used.
The yeast fermentation liquor adopts technical finesse generally in the art.
The preparation method of the aspergillus niger culture comprises the steps:Spawn culture, using conventional solid fermentation culture
Method:Spore liquid is inoculated in solid state fermentation culture material, is cultivated to mycelia for 26-33 DEG C and is covered with compost, and low temperature fluidized bed is dried,
Crush dried object.
Beneficial effect:
The biological compound fertilizer ﹑ composite fermentations of one kind that the present invention is provided have machine fertilizer material ﹑ mushroom bacteria rod ﹑ plant seed collaborations to control
The method in reason desert, loads non-woven bag, is prepared as lengths after above-mentioned substance is mixed in proportion;Non-woven fabrics are designed to
Preferably Xu Shui ﹑ moisturizings;And biological compound fertilizer ﹑ composite fermentations have machine fertilizer to expect that ﹑ intensive treatment mushroom bacteria rod can be plant speciess
Son provides sufficient nutritional labeling.Then the lengths are surrounded maximal side to set on three sides of 0.5-2m or quadrangular array
Put in pending desert section, fixing device fixed mesh band is arranged according to desert area wind-force, by means of nature or Artificial Rain
Water makes lengths humidity be adapted to plant seed germination, keeps lengths humidity in suitable seed germination scope.Plant is caused finally
The germination rate of seed is 97%, and survival rate 95% can effectively improve control of desert efficiency using the inventive method, improve green
Change effect, improve germination rate and survival rate 13-26%, later stage desert afforestation good stability.
In maize cob meal in biological compound fertilizer, crude fiber content is high, and lignocellulose degradation can be converted by microorganism aspergillus niger
Into sugared ﹑ fat and the nutritional labeling such as protein, while N-hydroxy-succinamide 1 is a kind of synthesizing amino acid protective agent, can be with
The nutrition of protected protein matter is not lost, and provides more nutrition for saccharomycetic fermentation.Biological compound fertilizer, intensive treatment mushroom bacteria
In rod, composite fermentation organic fertilizer, bacterium adds aspergillus niger culture, Aspergillus niger strain Aspergillus niger Li-
2013-03 is wheel more than the aspergillus niger Aspergllus niger Li-2010 Jing produced by one plant of cellulase-producing of Laboratories Accession
Nitrosoguanidine mutagenesis, then eliminate to mutant step-sizing, and finally the fermented performance test screening of strain excellent is produced
The Aspergillus niger strain of high activity cellulase, the cellulose that it can be efficiently in degrading maize core powder, crushing straw give birth to which
Into glucose, the fermentation for microorganism provides more nutrition.
In the yeast body that intensive treatment mushroom bacteria rod is added during preparation containing rich in protein, fat,
Sugar and vitamin B group etc., and enzyme, coenzyme, ribonucleic acid and some metabolic intermediate products, can improve fermented cow dung
Nutritive value;Fermentation of vinegar residue and aspergillus niger culture, fermented vinegar are also added in the sweat of composite fermentation fertilizer
In grain, crude fiber content is high, and lignocellulose degradation can be converted the nutritional labelings such as saccharogenesis ﹑ fat and protein by microorganism aspergillus niger,
Fermentation for microorganism provides more nutrition.
Contain crude protein 10%--20%, crude fat 1%--3%, nitrogen-free in the raw material cattle manure of composite fermentation organic fertilizer
Extractum 20%--30%, crude fibre 15%--30%, therefore with very high nutrient, can be fluffy with composite fermentation organic fertilizer
Soil, improves hardening soil situation, chelates the trace element in soil, lifts soil fertility etc.;Containing abundant in yeast body
Protein, fat, sugar and vitamin B group etc., and enzyme, coenzyme, ribonucleic acid and some metabolic intermediate products,
The nutritive value of fermented cow dung can be improved;Composite fermentation organic fertilizer adjust during preparation moisture build heap fermentation can protect
There are the moisture and anaerobic environment of abundance during card cattle manure so that cattle manure is more abundant;Composite fermentation fertilizer
Material is also added into Gypsum Fibrosum powder during preparation, its unique microcellular structure and thermal dehydration, is allowed to have excellent heat-insulated
And fire protecting performance, it is to avoid fermentation temperature is too high to cause burn to plant seed;In the sweat of composite fermentation fertilizer also
In the useless vinegar of the fermentation of addition, crude fiber content is high, and microorganism aspergillus niger can be by lignocellulose degradation conversion saccharogenesis ﹑ fat and egg
The nutritional labelings such as white matter, the fermentation for cattle manure provide more nutrition;The cyclodextrin energy being additionally added in the sweat of cattle manure
Increase the compatibility of various microorganisms in cattle manure, also act the effect of slow release;The shitosan added in fertilizer can be effective
Ground is coupled various nutrient substance becomes compact texture.The gamma-polyglutamic acid-added in the fermentation later stage of fertilizer is easily cross-linked to form
Later stage possesses the hydrogel of excellent performance, and gamma-polyglutamic acid-also has ultrahydrophilic and water holding capacity, unrestrained to flood in soil
When, can plant root hair top layer formed thin film, not only with protection root hair function, even more in soil nutrient, moisture content with
The optimal transport platform of root hair intimate contact, can the very efficient dissolving for improving fertilizer, storage, conveying and absorption;Prevent sulfur
Acid group, phosphate radical, oxalate and metallic element produce precipitation, enable crop more effectively absorb Soil Phosphorus, calcium, magnesium and
Trace element.Promote the development of crop root, strengthen disease resistance;Balanced soil pH-value, has excellent buffer capacity to acid, alkali
Power, can active balance soil PH, it is to avoid the acid soil property caused by life-time service chemical fertilizer;Can be poisonous heavy with reference to precipitating
Metal has splendid chelate effect to toxic heavy metals such as Pb2+, Cu2+, Cd2+, Cr3+, Al3+, As4+;Genes For Plant Tolerance can be strengthened
Sick and degeneration-resistant border ability.The water work composition in plant nutrient, soil is integrated, opposing can be strengthened by soilborne pathogenic institute
The symptom for causing, finally promotes emergence rate.
Description of the drawings
The present invention is further detailed explanation with reference to the accompanying drawings and detailed description.
Fig. 1 is that the triangle of the present invention fixes the sand the schematic diagram of nursery net.
Fig. 2 is that the tetragon of the present invention fixes the sand the schematic diagram of nursery net.
Reference name in figure corresponding to each sequence number is as follows:1 ﹑ fixes the sand band;2 ﹑ chocks;3 ﹑ grid cells.
Specific embodiment
By specifically describing, the method and device used of sand control in illustrating the present invention, are realized.To have by embodiment
Help understand the present invention, but do not limit present disclosure.One of ordinary skill in the art can be from present disclosure
The deformation used directly derived or associate, is considered as protection scope of the present invention.
It is prepared by aspergillus niger culture:Spawn culture, solid fermentation culture:Spore liquid is inoculated in solid state fermentation culture material,
Cultivate to mycelia for 26-33 DEG C and cover with compost, low temperature fluidized bed dry, pulverize dried object.
The Aspergillus niger strain Aspergillus niger Li-2013-03 that the present invention is provided are by the one of Laboratories Accession
Nitrosoguanidine mutagenesis being taken turns the aspergillus niger Aspergillus niger Li-2010 Jing that strain cellulase-producing is produced, then to mutation more
Strain step-sizing is eliminated, and finally the fermented performance test screening of strain excellent is obtained producing the Aspergillus niger of high activity cellulase
Strain Aspergillus niger Li-2013-03.
The bacterial strain of the high activity cellulase of product that the present invention is provided is specially aspergillus niger Aspergillus niger Li-
2013-03.The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organismss on July 15th, 2013
(abbreviation CGMCC, address is the heart:City of BeiJing, China Chaoyang District North Star West Road 1 institute 3,100101), preserving number is postcode
CGMCC NO.7927.The circumscribed 1,4 beta-glucanase of strains A spergillus niger Li-2013-03, inscribe beta glucan
Enzyme, beta-glucosidase and filter paper enzyme activity respectively reach 620U/mL, 1289U/mL, 456U/mL and 732U/mL, respectively than going out
Send out strains A spergillus niger Li-2010 and improve 9.21 times, 7.43 times, 8.15 times and 10.31 times.
Embodiment 1
A kind of method of Desert Control, comprises the steps:
By the biological compound fertilizer for preparing, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed according to than
Load non-woven fabrics to fix the sand band 1 after example mixing, it is described to fix the sand surround maximal side with 1 and be arranged in the quadrilateral mesh of 1m treat
Desert section is processed, fixing device is arranged according to desert area wind-force and is fixed the sand with 1.Made by means of natural or artificial rainwater solid
1 humidity of sand ribbon is adapted to plant seed germination, and holding fixes the sand 1 humidity of band in suitable seed germination and growth scope.The band 1 that fixes the sand is constituted
Grid cell 3 becomes the ultimate unit that fixes the sand.
The parts by weight of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
Composition is as follows:3 parts of biological compound fertilizer, 6 parts of intensive treatment mushroom bacteria rod, 20 parts of composite fermentation organic fertilizer, plant seed 0.5
Part, the plant is prairie milk vetch.
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:Yeast bacteria agent 25
Part, 5 parts of maize cob meal, 10 parts of aspergillus niger culture, 1 part of N-hydroxy-succinamide.
The preparation method of the intensive treatment mushroom bacteria rod is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition egg
Shell powder, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid, maintain the temperature at 25 DEG C after turning uniformly, and culture 10 is little
When after add aspergillus niger culture at 28 DEG C, cultivate 30 hours, keeping temperature turns mix homogeneously, and 10 DEG C of placements are stand-by.
The weight portion of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Array is into as follows:12 parts of crab flavour mushroom bacterium rod, 0.6 part of egg-shell meal, 4 parts of powder of straw, 6 parts of crushing maize core powder, 3 parts of fermentation of vinegar residue,
2 parts of yeast liquid.
The addition of the aspergillus niger culture for crab flavour mushroom bacterium rod quality 4%.Aspergillus niger is CGMCC NO.7927.
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation
Proportionally mix with cattle manure, addition compound bacteria adjusts moisture and builds heap fermentation, temperature is turned when reaching 65 DEG C once, continue to send out
Ferment, does not turn in sweat, after temperature reaches 72 DEG C keep 6 hours, addition cyclodextrin, shitosan, gamma-polyglutamic acid-after
Supervention ferment, holding turn at intervals of once, treated per 12 hours temperature reach 30 DEG C end fermentation process.
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Corn cob
6 parts of powder, 3 parts of crushing straw, 1 part of Gypsum Fibrosum powder, useless 3 parts of the vinegar of fermentation, 15 parts of cattle manure.
The compound bacteria component and parts by weight composition:1.5 parts of yeast liquid, 5 parts of aspergillus niger culture.
The addition of the compound bacteria for maize cob meal quality 1.5%.
The cyclodextrin addition for maize cob meal quality 2%.
The shitosan addition for maize cob meal quality 1%.
The gamma-polyglutamic acid-addition for maize cob meal quality 0.3%.
The cultural method of the yeast liquid comprises the steps:Using common saccharomycete seed liquor body cultural method:Will
It is stored in the yeast seeds in sand pipe, lyophilization pipe in a dormant state and accesses test tube slant activation, inoculation liquid training
Foster base culture 15 hours.
The yeast is CGMCC NO.12789.
The yeast fermentation liquor adopts technical finesse generally in the art.The preparation method of the aspergillus niger culture includes
Following steps:Spawn culture, using conventional solid fermentation culture method:Spore liquid is inoculated in solid state fermentation culture material, 26 trainings
Support to mycelia and cover with compost, low temperature fluidized bed dry, pulverize dried object.
The described nursery net that fixes the sand is constituted by the grid of 1*1M and for fixed chock 2.Grid is surrounded by the band 1 that fixes the sand,
Each fixes the sand and is fixed with 1 intersection timber wedge or ferrum wedge;
Experimental result:Germination rate is 92%, waters the cycle for 20 days, and plant survival rate reaches more than 91%.Water the cycle
The usage amount of interior water is per square metre of 2700 grams of water, and grid core is emptied as 8cm by the vortex that air-flow is produced;Plant after half a year
Thing realizes stably growth fixed mesh desert, and husky in grid to realize stablizing, plant growing is vigorous, it is no longer necessary to place of manually watering
Reason.2nd year grid realizes that green plantss cover.
Embodiment 2
A kind of method of Desert Control, comprises the steps:
By the biological compound fertilizer for preparing, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed according to than
Example mixing after load non-woven fabrics, with sewing machine be seamed into it is flat fix the sand band 1, it is described fix the sand with 1 surround maximal side 1.2m etc.
Side triangular mesh is arranged in pending desert section, arranges fixing device according to desert area wind-force and fixes the sand with 1.
The suitable plant seed germination of 1 humidity of band that fixes the sand, holding is made to fix the sand and sprout in suitable seed with 1 humidity by means of nature or artificial rainwater
Bud and growth scope.The band 1 that fixes the sand constitutes grid cell 3 becomes the ultimate unit that fixes the sand.
The parts by weight of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
Composition is as follows:2 parts of biological compound fertilizer, 10 parts of intensive treatment mushroom bacteria rod, 30 parts of composite fermentation organic fertilizer, plant seed 0.2
Part, the plant is Alfalfa.
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:Yeast bacteria agent 30
Part, 5 parts of maize cob meal, 12 parts of aspergillus niger culture, 1 part of N-hydroxy-succinamide.
The preparation method of the intensive treatment mushroom bacteria rod is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition egg
Shell powder, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid, maintain the temperature at 20 DEG C after turning uniformly, and culture 10 is little
When after add aspergillus niger culture at 30 DEG C, cultivate 28 hours, keeping temperature turns mix homogeneously, and 10 DEG C of placements are stand-by.
The weight portion of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Array is into as follows:15 parts of crab flavour mushroom bacterium rod, 0.5 part of egg-shell meal, 3 parts of powder of straw, 5 parts of crushing maize core powder, 5 parts of fermentation of vinegar residue,
1 part of yeast liquid.
The addition of the aspergillus niger culture for crab flavour mushroom bacterium rod quality 2%.
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation
Proportionally mix with cattle manure, addition compound bacteria adjusts moisture and builds heap fermentation, temperature is turned when reaching 65 DEG C once, continue to send out
Ferment, does not turn in sweat, after temperature reaches 70 DEG C keep 4 hours, addition cyclodextrin, shitosan, gamma-polyglutamic acid-after
Supervention ferment, holding turn at intervals of once, treated per 10 hours temperature reach 30 DEG C end fermentation process.
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Corn cob
8 parts of powder, 2 parts of crushing straw, 0.5 part of Gypsum Fibrosum powder, useless 5 parts of the vinegar of fermentation, 20 parts of cattle manure.
The compound bacteria component and parts by weight composition:2 parts of yeast liquid, 4 parts of aspergillus niger culture.
The addition of the compound bacteria for maize cob meal quality 1%.
The cyclodextrin addition for maize cob meal quality 1%.
The shitosan addition for maize cob meal quality 1%.
The gamma-polyglutamic acid-addition for maize cob meal quality 0.1%.
The cultural method of the yeast liquid comprises the steps:Using common saccharomycete seed liquor body cultural method:Will
It is stored in the yeast seeds in sand pipe, lyophilization pipe in a dormant state and accesses test tube slant activation, inoculation liquid training
Foster base culture 12 hours.
The yeast is saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and the bacterial strain is in guarantor
The China Committee for Culture Collection of Microorganisms's common micro-organisms center of on July 15th, 2016 is hidden in, deposit number is CGMCC
No.12789, preservation address:Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode
100101。
The most suitable growth pH of the saccharomyces cerevisiae tlj2016 is 6.0-6.5, and optimum growth temperature is 28-35 DEG C;
The saccharomyces cerevisiae tlj2016 is isolated below saccharomyces cerevisiae starting strain Jing from the orchard of one plant of Ningxia
Step is obtained:
The original strain that sets out → test tube activation → dithyl sulfate (DES) mutation → hypertonic plate screening → nitrosoguanidine
(NTG) secondary screening (producing GSH abilities) → mitotic stability test is screened → fermented to mutagenesis screening → hypertonic flat board primary dcreening operation → shaking flask.
Its glucose tolerance of bacterial strain tlj2016 obtained after mutation and L-Cysteine tolerance are obtained
To raising, cell density, another aspect L-Cysteine tolerance on the one hand can be improved under high concentration glucose culture
Raising be beneficial to GSH and synthesize in intracellular in a large number, so as to improve the ability of bacterial strain large-scale production GSH.
1st, saccharomyces cerevisiae provided by the present invention reaches 300g/L to the tolerance of glucose, beneficial to which in high concentration Portugal
GSH is produced under the conditions of grape sugar;
2nd, saccharomyces cerevisiae provided by the present invention reaches 3308mg/L in 5L fermentation cylinder for fermentation production GSH final concentrations;
3rd, the ability of saccharomyces cerevisiae tolerance L-Cysteine provided by the present invention is far above starting strain, in 5mmol/L
Slow growth is remained under L-Cysteine effect, remains to keep GSH to synthesize in a large number under the effect of 40mmol/L L-Cysteine;
4th, saccharomyces cerevisiae salt resistance ability provided by the present invention reaches 18%, is conducive to extending its application.
GSH capacity experimentals are produced in tlj2016 fermentations under the conditions of high sugar
(1) shake-flask culture
One ring of tlj2016 slant strains is taken, is accessed and 150rpm in the 250mL shaking flasks of 30mL Shake flask mediums is housed, 30 DEG C
Culture 30h obtains seed liquor;
Shake flask medium (g/L):(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, yeast powder 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0;
(2) 5L fermentor cultivation
Seed liquor is pressed into 10% inoculum concentration, is accessed in the fermentation tank equipped with 3L fermentation medium, 30 DEG C, ventilation 6L/
Min, tank pressure 0.03MPa, 500rpm carry out fermentation culture under the conditions of permanent pH6.0, when fermenting to 30h, disposably add final concentration
For the L-Cysteine of 25mmol/L, total fermentation time is 50h;
Fermentation medium (g/L):(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, yeast powder
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0;
After fermentation ends, the content for determining GSH in fermentation liquid is 3308mg/L.
L-Cysteine tolerance is tested
By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to
150rpm in shaking flask, 30 DEG C are cultivated, and the half Guang ammonia of L- of different final concentrations when culture is to 12h, is added in shaking flask
Acid, is further cultured for 10h, determines dry cell weight, as a result table 2,3;
Shake flask medium (g/L):(NH4)2SO46th, glucose 20, K2HPO4·3H2O 3、KH2PO40.5th, yeast powder 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0;
Table 2:Starting strain L-Cysteine tolerance
L-Cysteine concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
Starting strain dry weight g/L | 22.6 | 15.7 | 10.2 | 4.3 | 2.2 | 0.8 |
GSH concentration (mg/L) | 35.6 | 46.7 | 43.2 | 40.7 | 37.9 | 25.3 |
3 tlj2016L- cysteine tolerances of table
L-Cysteine concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
Tlj2016 dry weights g/L | 25.7 | 28.5 | 23.6 | 21.2 | 20.6 | 18.7 |
GSH concentration (mg/L) | 73.2 | 98.3 | 113.5 | 121.7 | 127.5 | 135.8 |
From the results shown in Table 2, for starting strain, in culture medium, add L-Cysteine, cell stops growing,
And start self-dissolving, cause GSH rate of increase to reduce with the rising of L-Cysteine concentration;And low concentration L-Cysteine
Under, tlj2016 still is able to slow growth, with the raising of L-Cysteine concentration, under the dry cell weight of tlj2016 bacterial strains is slow
Drop, and GSH concentration sustainable growths, this result pass through to add half Guang ammonia of precusor amino acids-L- in being beneficial to GSH production processes
Acid proposes the production for promoting GSH.
Salt resistance ability is tested
Take tlj2016 bacterium solutions 1mL inoculation strain in containing different NaCl concentrations (concentration gradients are 0%, 2%, 5%,
10%th, 15%, 10mL YPD fluid mediums (pH=6.5) 18%), is placed at 30 DEG C and cultivates 24h respectively, and each processes 3
Individual repetition.Respectively take 1ml samples bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml diluents solid in YPD
It is coated with body flat board, culture 36 hours (each dilution factor do 3 parallel) record is inverted in 30 DEG C of biochemical cultivation cases and calculates flat
Bacterium number number on plate.The results are shown in Table 4, it is known that the resistance to salinity of the bacterium is 18%, illustrates that tlj2016 not only can be in conventional ring
Survive in border, still there is under high salt conditions vigor, can be applicable to consumption sugar in the high salt food processing process such as soy sauce, curing food
Produce glutathion.
4 salt resistance ability of table detection (× 107cfu/ml)
The preparation method of the aspergillus niger culture comprises the steps:Spawn culture, using conventional solid fermentation culture
Method:Spore liquid is inoculated in solid state fermentation culture material, is cultivated to mycelia for 33 DEG C and is covered with compost, and low temperature fluidized bed is dried, powder
Broken dried object.
Experimental result:The cycle watered for 25 days, germination rate is 97%, and plant survival rate reaches more than 97%.Water the cycle
The usage amount of interior water is per square metre of 1600 grams of water, and grid core is emptied as 7cm by the vortex that air-flow is produced.Plant after half a year
Thing realizes stably growth fixed mesh desert, and husky in grid to realize stablizing, plant growing highly reaches more than 40cm, no longer needs
Manually to water process.Realize in 2nd year grid that green plantss cover and stable growth, it is no longer necessary to manual watering.
Embodiment 3 is basic with example 1,2
A kind of method of Desert Control, comprises the steps:
By the biological compound fertilizer for preparing, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed according to than
Load the flat band 1 that fixes the sand of non-woven fabrics after example mixing, described fixing the sand quadrilateral mesh spread configuration of the maximal side in 1m is surrounded with 1
In pending desert section, fixing device is arranged according to desert area wind-force and is fixed the sand with 1.By means of natural or artificial rainwater
The suitable plant seed germination of 1 humidity of band that fixes the sand, holding is made to fix the sand 1 humidity of band in suitable seed germination and growth scope.Fix the sand band 1
Composition grid cell 3 becomes the ultimate unit that fixes the sand.
The parts by weight of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
Composition is as follows:3 parts of biological compound fertilizer, 6 parts of intensive treatment mushroom bacteria rod, 20 parts of composite fermentation organic fertilizer, plant seed 0.5
Part, the plant is prairie milk vetch.
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:Yeast bacteria agent 25
Part, 5 parts of maize cob meal, 10 parts of aspergillus niger culture, 1 part of N-hydroxy-succinamide.
The preparation method of the intensive treatment mushroom bacteria rod is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition egg
Shell powder, powder of straw, crushing maize core powder, fermentation of vinegar residue and, turn it is uniform after maintain the temperature at 25 DEG C, culture was added after 10 hours
Aspergillus niger culture is cultivated 30 hours at 28 DEG C, and keeping temperature turns mix homogeneously, and 10 DEG C of placements are stand-by.
The weight portion of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Array is into as follows:12 parts of crab flavour mushroom bacterium rod, 0.6 part of egg-shell meal, 4 parts of powder of straw, 6 parts of crushing maize core powder, 3 parts of fermentation of vinegar residue.
The addition of the aspergillus niger culture for crab flavour mushroom bacterium rod quality 4%.
The preparation method of the aspergillus niger culture comprises the steps:Spawn culture, using conventional solid fermentation culture
Method:Spore liquid is inoculated in solid state fermentation culture material, is cultivated to mycelia for 26 DEG C and is covered with compost, and low temperature fluidized bed is dried, powder
Broken dried object.
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation
Proportionally mix with cattle manure, addition yeast liquid adjusts moisture and builds heap fermentation, temperature is turned when reaching 65 DEG C once, continue to send out
Ferment, does not turn in sweat, is kept for 6 hours after temperature reaches 72 DEG C, continues fermentation, and holding is turned at intervals of per 12 hours
Once, treat that temperature reaches 30 DEG C of end fermentation process.
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Corn cob
6 parts of powder, 3 parts of crushing straw, 1 part of Gypsum Fibrosum powder, useless 3 parts of the vinegar of fermentation, 15 parts of cattle manure.
The saccharomycetic addition for maize cob meal quality 1.5%.
The cultural method of the yeast liquid comprises the steps:Using common saccharomycete seed liquor body cultural method:Will
It is stored in the yeast seeds in sand pipe, lyophilization pipe in a dormant state and accesses test tube slant activation, inoculation liquid training
Foster base culture 15 hours.
The yeast is CICC1048.,
The described nursery net that fixes the sand is constituted by the grid of 1*1M and for fixed chock 2.Grid is surrounded by the band 1 that fixes the sand,
Each fixes the sand and is fixed with 1 intersection timber wedge or ferrum wedge.
Experimental result:Germination rate is 72%, waters the cycle for 20 days, and plant survival rate reaches more than 61%.Water the cycle
The usage amount of interior water is per square metre of 6700 grams of water, and grid core is emptied as 27cm by the vortex that air-flow is produced;After half a year
Plant can not realize stable growth fixed mesh desert, and grid is not carried out green plantss covering within the 2nd year.
Embodiment 4
A kind of method of Desert Control, comprises the steps:
By the biological compound fertilizer for preparing, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed according to than
Example mixing after load non-woven fabrics, with sewing machine be seamed into it is flat fix the sand band 1, it is described fix the sand with 1 surround maximal side 1.2m etc.
Side triangular mesh is arranged in pending desert section, arranges fixing device according to desert area wind-force and fixes the sand with 1.
The suitable plant seed germination of 1 humidity of band that fixes the sand, holding is made to fix the sand and sprout in suitable seed with 1 humidity by means of nature or artificial rainwater
Bud and growth scope.The band 1 that fixes the sand constitutes grid cell 3 becomes the ultimate unit that fixes the sand.
The parts by weight of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
Composition is as follows:2 parts of biological compound fertilizer, 10 parts of intensive treatment mushroom bacteria rod, 30 parts of composite fermentation organic fertilizer, plant seed 0.2
Part, the plant is Alfalfa.
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:Yeast bacteria agent 30
Part, maize cob meal 5,12 parts of aspergillus niger culture.
The preparation method of the reinforcing mushroom bacteria rod:Crab flavour mushroom bacterium rod after culture sporophore crushes addition egg-shell meal, straw
Stalk powder, crushing maize core powder, fermentation of vinegar residue and yeast liquid, maintain the temperature at 25 DEG C after turning uniformly, cultivate 38 hours, 10 DEG C
Place stand-by.
The weight portion of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Array is into as follows:15 parts of crab flavour mushroom bacterium rod, 0.5 part of egg-shell meal, 3 parts of powder of straw, 5 parts of crushing maize core powder, 5 parts of fermentation of vinegar residue,
1 part of yeast liquid.
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation
Proportionally mix with cattle manure, addition compound bacteria adjusts moisture and builds heap fermentation, temperature is turned when reaching 65 DEG C once, continue to send out
Ferment, does not turn in sweat, after temperature reaches 70 DEG C keep 4 hours, addition cyclodextrin, shitosan, gamma-polyglutamic acid-after
Supervention ferment, holding turn at intervals of once, treated per 10 hours temperature reach 30 DEG C end fermentation process.
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Corn cob
8 parts of powder, 2 parts of crushing straw, 0.5 part of Gypsum Fibrosum powder, useless 5 parts of the vinegar of fermentation, 20 parts of cattle manure.
The compound bacteria component and parts by weight composition:2 parts of yeast liquid, 4 parts of aspergillus niger culture.
The addition of the compound bacteria for maize cob meal quality 1%.
The cyclodextrin addition for maize cob meal quality 1%.
The shitosan addition for maize cob meal quality 1%.
The gamma-polyglutamic acid-addition for maize cob meal quality 0.1%.
The cultural method of the yeast liquid comprises the steps:Using common saccharomycete seed liquor body cultural method:Will
It is stored in the yeast seeds in sand pipe, lyophilization pipe in a dormant state and accesses test tube slant activation, inoculation liquid training
Foster base culture 20 hours.
The yeast is yeast CGMCC NO.12789.
The yeast fermentation liquor adopts technical finesse generally in the art.
The preparation method of the aspergillus niger culture comprises the steps:Spawn culture, using conventional solid fermentation culture
Method:Spore liquid is inoculated in solid state fermentation culture material, is cultivated to mycelia for 33 DEG C and is covered with compost, and low temperature fluidized bed is dried, powder
Broken dried object.
Experimental result:Germination rate is 86%, waters the cycle for 25 days, and plant survival rate reaches 89%.Water water in the cycle
Usage amount be per square metre of 2600 grams of water, grid core is emptied as 9cm by the vortex that air-flow is produced.Plant reality after half a year
Now stable to grow and fixed mesh desert, in grid, husky realization is stable, and plant growing highly reaches 30cm, it is no longer necessary to manually pour
Water process.Realize in 2nd year grid that green plantss cover and stable growth.
Embodiment 5
A kind of method of Desert Control, comprises the steps:
By the biological compound fertilizer for preparing, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed according to than
Example mixing after load non-woven fabrics it is flat fix the sand band 1, it is described fix the sand band 1 surround maximal side 0.5m quadrilateral mesh arrange set
Put in pending desert section, fixing device is arranged according to desert area wind-force and is fixed the sand with 1.By means of nature or Artificial Rain
Water makes the suitable plant seed germination of 1 humidity of band that fixes the sand, holding fix the sand 1 humidity of band in suitable seed germination and growth scope.Fix the sand
Band 1 constitutes grid cell 3 becomes the ultimate unit that fixes the sand.
The parts by weight of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
Composition is as follows:5 parts of biological compound fertilizer, 5 parts of intensive treatment mushroom bacteria rod, 15 parts of composite fermentation organic fertilizer, 1 part of plant seed,
The plant is Alfalfa.
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:Yeast bacteria agent 20
Part, 3 parts of maize cob meal, 8 parts of aspergillus niger culture, 1 part of N-hydroxy-succinamide.
The yeast bacteria agent is commercially available active dry yeast.
The preparation method of the intensive treatment mushroom bacteria rod is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition egg
Shell powder, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid, maintain the temperature at 30 DEG C after turning uniformly, and culture 8 is little
When after add aspergillus niger culture at 25 DEG C, cultivate 18 hours, keeping temperature turns mix homogeneously, and 10 DEG C of placements are stand-by.
The weight portion of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Array is into as follows:8 parts of crab flavour mushroom bacterium rod, 1 part of egg-shell meal, 8 parts of powder of straw, 10 parts of crushing maize core powder, 2 parts of fermentation of vinegar residue, ferment
3 parts of mother bacterium fluid.
The addition of the aspergillus niger culture for crab flavour mushroom bacterium rod quality 5%.
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation
Proportionally mix with cattle manure, addition compound bacteria adjusts moisture and builds heap fermentation, temperature is turned when reaching 65 DEG C once, continue to send out
Ferment, does not turn in sweat, is kept for 10 hours after temperature reaches 80 DEG C, addition cyclodextrin, shitosan, gamma-polyglutamic acid-
Continue fermentation, holding turn at intervals of once, treated per 20 hours temperature reach 25 DEG C end fermentation process.
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Corn cob
4 parts of powder, 4 parts of crushing straw, 2 parts of Gypsum Fibrosum powder, useless 2 parts of the vinegar of fermentation, 10 parts of cattle manure.
The compound bacteria component and parts by weight composition:1 part of yeast liquid, 8 parts of aspergillus niger culture.
The addition of the compound bacteria for maize cob meal quality 2%.
The cyclodextrin addition for maize cob meal quality 3%.
The shitosan addition for maize cob meal quality 2%.
The gamma-polyglutamic acid-addition for maize cob meal quality 0.5%.
The cultural method of the yeast liquid comprises the steps:Using common saccharomycete seed liquor body cultural method:Will
It is stored in the yeast seeds in sand pipe, lyophilization pipe in a dormant state and accesses test tube slant activation, inoculation liquid training
Foster base culture 15 hours.
The yeast is yeast CGMCC NO.12789.
The yeast fermentation liquor adopts technical finesse generally in the art.
The preparation method of the aspergillus niger culture comprises the steps:Spawn culture, using conventional solid fermentation culture
Method:Spore liquid is inoculated in solid state fermentation culture material, is cultivated to mycelia for 26 DEG C and is covered with compost, and low temperature fluidized bed is dried, powder
Broken dried object.
The described nursery net that fixes the sand is constituted by the grid of 0.5*0.5M and for fixed chock 2.Grid is enclosed by the band 1 that fixes the sand
Into each fixes the sand and is fixed with 1 intersection timber wedge or ferrum wedge;
Experimental result:Germination rate is 93%, waters the cycle for 20 days, and plant survival rate reaches more than 93%.Water the cycle
The usage amount of interior water is per square metre of 2300 grams of water, and grid core is emptied as 7cm by the vortex that air-flow is produced;Plant after half a year
Thing realizes stably growth fixed mesh desert, and husky in grid to realize stablizing, plant growing is vigorous, it is no longer necessary to place of manually watering
Reason.2nd year grid realizes that green plantss cover.
Embodiment 6
A kind of method of Desert Control, comprises the steps:
By the biological compound fertilizer for preparing, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed according to than
Load the flat band 1 that fixes the sand of non-woven fabrics after example mixing, described fixing the sand quadrilateral mesh spread configuration of the maximal side in 2m is surrounded with 1
In pending desert section, fixing device is arranged according to desert area wind-force and is fixed the sand with 1.By means of natural or artificial rainwater
The suitable plant seed germination of 1 humidity of band that fixes the sand, holding is made to fix the sand 1 humidity of band in suitable seed germination and growth scope.Fix the sand band 1
Composition grid cell 3 becomes the ultimate unit that fixes the sand.
The parts by weight of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
Composition is as follows:5 parts of biological compound fertilizer, 5 parts of intensive treatment mushroom bacteria rod, 15 parts of composite fermentation organic fertilizer, 1 part of plant seed,
The plant is Alfalfa.
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:Yeast bacteria agent 35
Part, 6 parts of maize cob meal, 15 parts of aspergillus niger culture, 1 part of N-hydroxy-succinamide.
The preparation method of the intensive treatment mushroom bacteria rod is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition egg
Shell powder, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid, maintain the temperature at 30 DEG C after turning uniformly, and culture 15 is little
When after add aspergillus niger culture at 25 DEG C, cultivate 40 hours, keeping temperature turns mix homogeneously, and 10 DEG C of placements are stand-by.
The weight portion of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Array is into as follows:8 parts of crab flavour mushroom bacterium rod, 1 part of egg-shell meal, 8 parts of powder of straw, 10 parts of crushing maize core powder, 2 parts of fermentation of vinegar residue, ferment
3 parts of mother bacterium fluid.
The addition of the aspergillus niger culture for crab flavour mushroom bacterium rod quality 5%.
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation
Proportionally mix with cattle manure, addition compound bacteria adjusts moisture and builds heap fermentation, temperature is turned when reaching 65 DEG C once, continue to send out
Ferment, does not turn in sweat, is kept for 10 hours after temperature reaches 80 DEG C, addition cyclodextrin, shitosan, gamma-polyglutamic acid-
Continue fermentation, holding turn at intervals of once, treated per 20 hours temperature reach 40 DEG C end fermentation process.
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Corn cob
4 parts of powder, 4 parts of crushing straw, 2 parts of Gypsum Fibrosum powder, useless 2 parts of the vinegar of fermentation, 10 parts of cattle manure.
The compound bacteria component and parts by weight composition:1 part of yeast liquid, 8 parts of aspergillus niger culture.
The addition of the compound bacteria for maize cob meal quality 2%.
The cyclodextrin addition for maize cob meal quality 3%.
The shitosan addition for maize cob meal quality 2%.
The gamma-polyglutamic acid-addition for maize cob meal quality 0.5%.
The cultural method of the yeast liquid comprises the steps:Using common saccharomycete seed liquor body cultural method:Will
It is stored in the yeast seeds in sand pipe, lyophilization pipe in a dormant state and accesses test tube slant activation, inoculation liquid training
Foster base culture 15 hours.
The yeast is yeast CGMCC NO.12789.
The yeast fermentation liquor adopts technical finesse generally in the art.
The preparation method of the aspergillus niger culture comprises the steps:Spawn culture, using conventional solid fermentation culture
Method:Spore liquid is inoculated in solid state fermentation culture material, is cultivated to mycelia for 26 DEG C and is covered with compost, and low temperature fluidized bed is dried, powder
Broken dried object.
The described nursery net that fixes the sand is constituted by the grid of 2*2M and for fixed chock 2.Grid is surrounded by the band 1 that fixes the sand,
Each fixes the sand and is fixed with 1 intersection timber wedge or ferrum wedge;
Experimental result:Germination rate is 88%, waters the cycle for 20 days, and plant survival rate reaches more than 90%.Water the cycle
The usage amount of interior water is per square metre of 4000 grams of water, and grid core is emptied as 12cm by the vortex that air-flow is produced;After half a year
Plant realizes stably growth fixed mesh desert, and husky in grid to realize stablizing, plant growing is vigorous, it is no longer necessary to manually water
Process.2nd year grid realizes that green plantss cover.
Claims (10)
1. a kind of method of Desert Control, comprises the steps:
The biological compound fertilizer of preparation, intensive treatment mushroom bacteria rod, composite fermentation You machine Fei Liao ﹑ plant seeds are proportionally mixed
After load non-woven fabrics, be seamed into the flat band (1) that fixes the sand with sewing machine, the band (1) that fixes the sand surrounds three sides or quadrilateral mesh is continuous
Pending desert section is arranged in, fixing device is arranged according to desert area wind-force and is fixed the sand with (1);By means of nature
Or artificial rainwater makes the suitable plant seed germination of band (2) humidity that fixes the sand, holding fix the sand band (2) humidity in suitable seed germination and life
Long scope, the band (1) composition grid cell (3) that fixes the sand become the ultimate unit that fixes the sand.
2. the method for a kind of Desert Control according to claim 1, it is characterised in that at the biological compound fertilizer, reinforcing
Reason mushroom bacteria rod, composite fermentation constitute as follows by the parts by weight of machine Fei Liao ﹑ plant seeds:At biological compound fertilizer 2-5 parts, reinforcing
Reason mushroom bacteria rod 5-10 parts, composite fermentation organic fertilizer 15-30 parts, plant seed 0.2-1 parts.
3. a kind of method of Desert Control according to claim 1 and 2, it is characterised in that the biological compound fertilizer weight
Number composition is as follows:Yeast bacteria agent 20-35 parts, maize cob meal 3-6 parts, aspergillus niger culture 8-15 part, N- hydroxysuccinimidyl acyls
Imines 1-2 parts.
4. the method for a kind of Desert Control according to claim 1 and 2, it is characterised in that the reinforcing mushroom bacteria rod
Preparation method is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition egg-shell meal, powder of straw, crushing maize core powder, fermentation
Vinegar and yeast liquid, maintain the temperature at 20-30 DEG C after turning uniformly, add aspergillus niger culture mixing after culture 8-15 hours
Afterwards at 25-30 DEG C, after culture 18-40 hours, keeping temperature turns mix homogeneously, and 10 DEG C of placements are stand-by.
5. the method for a kind of Desert Control according to claim 4, it is characterised in that the crab flavour mushroom bacterium rod, egg-shell meal,
The parts by weight composition of powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid is as follows:Crab flavour mushroom bacterium rod 8-15 parts, egg
Shell powder 0.5-1 parts, powder of straw 3-8 part, crushing maize core powder 5-10 parts, fermentation of vinegar residue 2-5 parts, yeast liquid 1-3 part;It is described black
2-5% of the addition of aspergillosis culture for crab flavour mushroom bacterium rod quality.
6. a kind of method of Desert Control according to claim 1 and 2, it is characterised in that the composite fermentation fertilizer
Preparation method for material is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure proportionally mix, and addition is compound
Bacterium adjusts moisture and builds heap fermentation, and temperature is turned when reaching 65 DEG C once, is continued fermentation, is not turned, treat that temperature reaches in sweat
To after 70-80 DEG C keep 4-10 hours, addition cyclodextrin, shitosan, gamma-polyglutamic acid-continue fermentation, holding turn at intervals of
Per 10-20 hours once, treat that temperature reaches 25-40 DEG C of end fermentation process.
7. the method for a kind of Desert Control according to claim 6, it is characterised in that the maize cob meal, crushing straw,
The parts by weight of Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Maize cob meal 4-8 parts, crushing straw 2-4 parts, Gypsum Fibrosum powder
0.5-2 parts, the useless vinegar 2-5 parts of fermentation, cattle manure 10-20 parts;
The compound bacteria component and parts by weight composition:Yeast liquid 1-2 part, aspergillus niger culture 4-8 part;
1-2% of the addition of the compound bacteria for maize cob meal quality;
1-3% of the cyclodextrin addition for maize cob meal quality;
1-2% of the shitosan addition for maize cob meal quality;
0.1-0.5% of the gamma-polyglutamic acid-addition for maize cob meal quality.
8. a kind of method of the Desert Control according to claim 5 or 7, it is characterised in that the ferment in the yeast liquid
Female bacterium is CGMCC NO.12789;Aspergillus niger (Aspergillus niger) Li-2013-03 in the aspergillus niger culture,
Preserving number is CGMCC NO.7927.
9. the method for a kind of Desert Control according to claim 1 and 2, it is characterised in that comprise the steps:
The biological compound fertilizer of preparation, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed are proportionally mixed
Load non-woven fabrics after conjunction, with sewing machine be seamed into it is flat fix the sand band (1), it is described fix the sand band (1) surround maximal side 1.2m etc.
Side triangular mesh is arranged in pending desert section, arranges fixing device according to desert area wind-force and fixes the sand band
(1);The suitable plant seed germination of band (1) humidity that fixes the sand, holding is made to fix the sand band (1) humidity suitable by means of natural or artificial rainwater
Close seed germination and growth scope;The band (1) composition grid cell (3) that fixes the sand becomes the ultimate unit that fixes the sand;
The parts by weight composition of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
It is as follows:2 parts of biological compound fertilizer, 10 parts of intensive treatment mushroom bacteria rod, 30 parts of composite fermentation organic fertilizer, 0.2 part of plant seed, institute
Plant is stated for Alfalfa;
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:30 parts of yeast bacteria agent,
5 parts of maize cob meal, 12 parts of aspergillus niger culture, 1 part of N-hydroxy-succinamide;
The preparation method of the intensive treatment mushroom bacteria rod is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition eggshell
Powder, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid, maintain the temperature at 20 DEG C after turning uniformly, cultivate 10 hours
Add aspergillus niger culture afterwards at 30 DEG C, cultivate 28 hours, keeping temperature turns mix homogeneously, and 10 DEG C of placements are stand-by;
The weight portion array of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Into as follows:15 parts of crab flavour mushroom bacterium rod, 0.5 part of egg-shell meal, 3 parts of powder of straw, 5 parts of crushing maize core powder, 5 parts of fermentation of vinegar residue, yeast
1 part of bacterium solution;The addition of the aspergillus niger culture for crab flavour mushroom bacterium rod quality 2%;
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle
Excrement proportionally mixes, and addition compound bacteria adjusts moisture and builds heap fermentation, and temperature is turned when reaching 65 DEG C once, continues fermentation, is sent out
Do not turn during ferment, kept for 4 hours after temperature reaches 70 DEG C, addition cyclodextrin, shitosan, gamma-polyglutamic acid-continue to send out
Ferment, holding turn at intervals of once, treated per 10 hours temperature reach 30 DEG C end fermentation process;
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Maize cob meal 8
Part, 2 parts of crushing straw, 0.5 part of Gypsum Fibrosum powder, useless 5 parts of the vinegar of fermentation, 20 parts of cattle manure;
The compound bacteria component and parts by weight composition:2 parts of yeast liquid, 4 parts of aspergillus niger culture;
The addition of the compound bacteria for maize cob meal quality 1%;
The cyclodextrin addition for maize cob meal quality 1%;
The shitosan addition for maize cob meal quality 1%;
The gamma-polyglutamic acid-addition for maize cob meal quality 0.1%;
The yeast is saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and deposit number is CGMCC
No.12789。
10. the method for a kind of Desert Control according to claim 1 and 2, it is characterised in that comprise the steps:
The biological compound fertilizer of preparation, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed are proportionally mixed
After conjunction load non-woven fabrics fix the sand band 1, it is described fix the sand with 1 surround maximal side 1m quadrilateral mesh be arranged in it is pending
Desert section, arranges fixing device according to desert area wind-force and fixes the sand with 1.The band that fixes the sand is made by means of natural or artificial rainwater
1 humidity is adapted to plant seed germination, and holding fixes the sand 1 humidity of band in suitable seed germination and growth scope.The band 1 that fixes the sand constitutes grid
Unit 3 becomes the ultimate unit that fixes the sand;
The parts by weight composition of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
It is as follows:3 parts of biological compound fertilizer, 6 parts of intensive treatment mushroom bacteria rod, 20 parts of composite fermentation organic fertilizer, 0.5 part of plant seed, institute
Plant is stated for prairie milk vetch;
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:25 parts of yeast bacteria agent,
5 parts of maize cob meal, 10 parts of aspergillus niger culture, 1 part of N-hydroxy-succinamide;
The preparation method of the intensive treatment mushroom bacteria rod is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition eggshell
Powder, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid, maintain the temperature at 25 DEG C after turning uniformly, cultivate 10 hours
Add aspergillus niger culture afterwards at 28 DEG C, cultivate 30 hours, keeping temperature turns mix homogeneously, and 10 DEG C of placements are stand-by;
The weight portion array of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Into as follows:12 parts of crab flavour mushroom bacterium rod, 0.6 part of egg-shell meal, 4 parts of powder of straw, 6 parts of crushing maize core powder, 3 parts of fermentation of vinegar residue, yeast
2 parts of bacterium solution;
The addition of the aspergillus niger culture for crab flavour mushroom bacterium rod quality 4%.Aspergillus niger is CGMCC NO.7927;
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle
Excrement proportionally mixes, and addition compound bacteria adjusts moisture and builds heap fermentation, and temperature is turned when reaching 65 DEG C once, continues fermentation, is sent out
Do not turn during ferment, kept for 6 hours after temperature reaches 72 DEG C, addition cyclodextrin, shitosan, gamma-polyglutamic acid-continue to send out
Ferment, holding turn at intervals of once, treated per 12 hours temperature reach 30 DEG C end fermentation process;
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Maize cob meal 6
Part, 3 parts of crushing straw, 1 part of Gypsum Fibrosum powder, useless 3 parts of the vinegar of fermentation, 15 parts of cattle manure;
The compound bacteria component and parts by weight composition:1.5 parts of yeast liquid, 5 parts of aspergillus niger culture;
The addition of the compound bacteria for maize cob meal quality 1.5%;
The cyclodextrin addition for maize cob meal quality 2%;
The shitosan addition for maize cob meal quality 1%;
The gamma-polyglutamic acid-addition for maize cob meal quality 0.3%;
The cultural method of the yeast liquid comprises the steps:Using common saccharomycete seed liquor body cultural method:To preserve
Yeast seeds in sand pipe, lyophilization pipe in a dormant state access test tube slant activation, are inoculated with fluid medium
Culture 15 hours;
The yeast is CGMCC NO.12789;
A kind of method of Desert Control, comprises the steps:
The biological compound fertilizer of preparation, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed are proportionally mixed
Loading non-woven fabrics after conjunction, the flat band 1 that fixes the sand being seamed into sewing machine, described fixing the sand surround maximal side equilateral the three of 1.2m with 1
Hexagonal lattice is arranged in pending desert section, arranges fixing device according to desert area wind-force and fixes the sand with 1;By
Be adapted to plant seed germination in natural or artificial rainwater 1 humidity of band that makes to fix the sand, holding fix the sand 1 humidity of band suitable seed germination with
Growth scope.The band 1 that fixes the sand constitutes grid cell 3 becomes the ultimate unit that fixes the sand;
The parts by weight composition of the biological compound fertilizer, intensive treatment mushroom bacteria rod, composite fermentation organic fertilizer and plant seed
It is as follows:2 parts of biological compound fertilizer, 10 parts of intensive treatment mushroom bacteria rod, 30 parts of composite fermentation organic fertilizer, 0.2 part of plant seed, institute
Plant is stated for Alfalfa;
The biological compound fertilizer preparation method is as follows:A kind of biological compound fertilizer, parts by weight are consisted of:30 parts of yeast bacteria agent,
5 parts of maize cob meal, 12 parts of aspergillus niger culture, 1 part of N-hydroxy-succinamide;
The preparation method of the intensive treatment mushroom bacteria rod is as follows:Crab flavour mushroom bacterium rod after culture sporophore crushes addition eggshell
Powder, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid, maintain the temperature at 20 DEG C after turning uniformly, cultivate 10 hours
Add aspergillus niger culture afterwards at 30 DEG C, cultivate 28 hours, keeping temperature turns mix homogeneously, and 10 DEG C of placements are stand-by;
The weight portion array of the crab flavour mushroom bacterium rod, egg-shell meal, powder of straw, crushing maize core powder, fermentation of vinegar residue and yeast liquid
Into as follows:15 parts of crab flavour mushroom bacterium rod, 0.5 part of egg-shell meal, 3 parts of powder of straw, 5 parts of crushing maize core powder, 5 parts of fermentation of vinegar residue, yeast
1 part of bacterium solution;
The addition of the aspergillus niger culture for crab flavour mushroom bacterium rod quality 2%;
The composite fermentation organic fertilizer preparation method is as follows:Maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle
Excrement proportionally mixes, and addition compound bacteria adjusts moisture and builds heap fermentation, and temperature is turned when reaching 65 DEG C once, continues fermentation, is sent out
Do not turn during ferment, kept for 4 hours after temperature reaches 70 DEG C, addition cyclodextrin, shitosan, gamma-polyglutamic acid-continue to send out
Ferment, holding turn at intervals of once, treated per 10 hours temperature reach 30 DEG C end fermentation process;
The parts by weight of the maize cob meal, crushing straw, Gypsum Fibrosum powder, the useless vinegar of fermentation and cattle manure are consisted of:Maize cob meal 8
Part, 2 parts of crushing straw, 0.5 part of Gypsum Fibrosum powder, useless 5 parts of the vinegar of fermentation, 20 parts of cattle manure;
The compound bacteria component and parts by weight composition:2 parts of yeast liquid, 4 parts of aspergillus niger culture;
The addition of the compound bacteria for maize cob meal quality 1%;The cyclodextrin addition is maize cob meal quality
1%;
The shitosan addition for maize cob meal quality 1%;The gamma-polyglutamic acid-addition is maize cob meal quality
0.1%;
The cultural method of the yeast liquid comprises the steps:Using common saccharomycete seed liquor body cultural method:To preserve
Yeast seeds in sand pipe, lyophilization pipe in a dormant state access test tube slant activation, are inoculated with fluid medium
Culture 12 hours;
The yeast is CGMCC No.12789.
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CN109220076A (en) * | 2018-08-14 | 2019-01-18 | 江苏沿海地区农业科学研究所 | A kind of Sesbania seed processing method |
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