CN106520561A - Method for manufacturing culture medium of sparassis crispa - Google Patents
Method for manufacturing culture medium of sparassis crispa Download PDFInfo
- Publication number
- CN106520561A CN106520561A CN201610807503.9A CN201610807503A CN106520561A CN 106520561 A CN106520561 A CN 106520561A CN 201610807503 A CN201610807503 A CN 201610807503A CN 106520561 A CN106520561 A CN 106520561A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- mentioned
- culture
- aminoacid
- vitamin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention relates to a method for preparing a culture medium for Sparassis crispa. According to the present invention, provided is a method for preparing a culture medium for Sparassis crispa comprising the following steps: preparing fermented sawdust; making a medium for culturing spawn by mixing the fermented sawdust with an antioxidant and amino acid, separately; sterilizing the medium; inoculating Sparassis crispa spawn into the medium; and culturing the spawn ininoculate into the medium.
Description
Technical field
The compound method of the Sparassia crispa (Wulf.) Fr. culture culture medium of antioxidant, aminoacid and vitamin is the present invention relates to the use of,
Relate more specifically to following method, i.e. can pass through to remove the active oxygen that culture medium is accumulated in incubation, shorten culture
The compound method of the Sparassia crispa (Wulf.) Fr. culture culture medium of the utilization antioxidant, aminoacid and vitamin of time.
Background technology
Sparassia crispa (Wulf.) Fr. (Sparassis crispa Wulf.:Fr.) be basidiomycetes Aphyllophorales silk ball Cordycepps funguses.Embroider
The sporophore of coccus is white, the flower shape of undulate, in summer to consor between autumn in the vicinity of tree root living, withered
Dead branch, stub etc., and made a living with saprogenesis.
It is used to eat because the meat of Sparassia crispa (Wulf.) Fr. is hard and in good taste, and as various effects of Sparassia crispa (Wulf.) Fr. are recognized,
Therefore be also used for it is medicinal, at present, it is most of by indoor artificial culture being produced.
In the past, the main bottle using using wood flour plants mode, but exist incubation time length, be difficult to produce in batches, yield
Low shortcoming.Also, in incubation, nutrient is put into improve growth efficiency, but is shortening incubation time
There is limitation in aspect.
In No. 1385540 (day for announcing of Korean granted patent：On 04 15th, 2014) disclosed in have as the present invention
Background technology.
The content of the invention
It is an object of the present invention to provide suppress aging by providing the culture environment for removing active oxygen, promote growth,
So as to the preparation side of the Sparassia crispa (Wulf.) Fr. culture culture medium of utilization antioxidant, aminoacid and the vitamin of incubation time can be shortened
Method.
In the present invention by removing in the incubation in limited container issuable active oxygen shortening training
It is in the compound method of the Sparassia crispa (Wulf.) Fr. culture culture medium of foster time, there is provided the compound method of Sparassia crispa (Wulf.) Fr. culture culture medium, above-mentioned
The compound method of Sparassia crispa (Wulf.) Fr. culture culture medium includes：By mixing antioxidant, ammonia respectively in the fermentation wood flour being ready for
Base acid and vitamin, the step of prepare spawn culture culture medium；The step of sterilization processing is carried out to above-mentioned culture medium；Upper
The step of stating inoculation Sparassia crispa (Wulf.) Fr. strain in culture medium；And cultivate the step of being inoculated in the above-mentioned strain in above-mentioned culture medium.
Wherein, in the step of above-mentioned preparation culture medium, can be in above-mentioned fermentation wood flour respectively with 1:In 1 ratio mixing
State antioxidant and above-mentioned aminoacid.Also, in the step of above-mentioned preparation culture medium, can in the above-mentioned fermentation wood flour respectively with
1:1:1 ratio mixes above-mentioned antioxidant, above-mentioned aminoacid and said vitamin.
Also, in the step of above-mentioned preparation culture medium, can in the above-mentioned fermentation wood flour respectively melting concn be 10 to
The above-mentioned antioxidant of 25ppm, above-mentioned aminoacid and said vitamin, above-mentioned antioxidant can be glutathion
(glutathione), above-mentioned aminoacid can be histidine (histidine), and said vitamin can be ascorbic acid
(ascorbic acid) or vitamin C.
The preparation side of the Sparassia crispa (Wulf.) Fr. culture culture medium of utilization antioxidant of the invention, aminoacid and vitamin
Method, has the advantage that, i.e. cultivates strain by using the culture medium of antioxidant, aminoacid and vitamin is included, goes
Except the active oxygen that culture medium is accumulated in incubation, so as to promote the fruiting of funguses, shorten incubation time, therefore can be short
In time, the high high-quality of batch production commodity value is individual.
Description of the drawings
Streams of the Fig. 1 for the Sparassia crispa (Wulf.) Fr. cultural method of the utilization antioxidant, aminoacid and vitamin of embodiments of the invention
Cheng Tu；
Fig. 2 is the figure for illustrating the shape for generating former base in culture vessel by the cultural method of embodiments of the invention；
Fig. 3 is the figure of the Sparassia crispa (Wulf.) Fr. for illustrating that embodiments in accordance with the present invention finally grow.
Specific embodiment
Hereinafter, referring to the drawings embodiments of the invention are described in detail, so that the technical field of the invention
Those of ordinary skill easily implements.
The present invention relates to the use of the compound method of the Sparassia crispa (Wulf.) Fr. culture culture medium of antioxidant, aminoacid and vitamin.
In an embodiment of the present invention, by the cultivation strain in the environment of culture medium of active oxygen is removed, growth efficiency is improved, from
And incubation time can be shortened, the high individuality of commodity price can be produced at short notice in batches.
Hereinafter, the cultural method of the Sparassia crispa (Wulf.) Fr. of embodiments of the invention is described in detail.Fig. 1 is the reality of the present invention
The flow chart for applying the Sparassia crispa (Wulf.) Fr. cultural method of utilization antioxidant, aminoacid and the vitamin of example.
First, prepare for preparing the fermentation wood flour (step S110) of culture medium.
Wherein, the wood flour that ferments can use the larch sawdust of customary by fermentation.Specifically, from larch sawdust
After removing oxybenzene compound, for cellulosic softness, ferment in open country and use afterwards for 6 months.The fermentation being ready for
Wood flour is adjustable as the moisture of 60～70% (except 65%).
Afterwards, by fermentation wood flour in mix antioxidant, aminoacid and vitamin respectively prepare spawn culture use
Culture medium (step S120).Wherein, glutathion can be used as antioxidant composition, can be using group ammonia as aminoacid ingredient
Sour (except L-Histidine), can use ascorbic acid or vitamin C as vitamin ingredients.Hereinafter, for convenience of description, illustrate
Go out using ascorbic acid as vitamin ingredients come the culture medium for using.
In the step of preparing culture medium, can be with 1 in fermentation wood flour:1 ratio (weight ratio) puts into and mixes anti-
Oxidant (glutathion) and aminoacid (histidine).Also, in the step of preparing culture medium, can be with fermentation wood flour
1:1:1 ratio (weight ratio) puts into and mixes antioxidant (glutathion), aminoacid (histidine) and vitamin (Vitamin C
Acid).Because the input ratio between the material that added is identical, therefore culture medium is easily prepared.
It is, however, preferable that the concentration of antioxidant, aminoacid and vitamin is within the scope of 10ppm to 25ppm.
Here the concentration of input antioxidant, aminoacid and vitamin may imply that be diluted in the solvent of water etc. it is above-mentioned dense
Degree.In an embodiment of the present invention, when the input concentration of antioxidant, aminoacid and vitamin is less than 10ppm or is more than 25ppm
When, growth rate is slow and growth length is short on the contrary, so as to reduce production efficiency.By following experimental result to related to this
Content is described in detail.
Like this, using the culture for being added with antioxidant and aminoacid or antioxidant and aminoacid and vitamin
In the case of base, the active oxygen of the reason for becoming aging because accumulating on culture medium in incubation can be effectively removed, so that
The environment from fruiting to development can be improved, so as to the speed of growth of the Sparassia crispa (Wulf.) Fr. according to these culture medium can be improved.
Secondly, the culture medium to having prepared carries out sterilization processing (step S130).Sterilization processing is to be put into kill culture medium
After bacterium pot, thoroughly sterilized by the method for steamed.Thus harmful bacteria is removed, and can prepare aseptic condition
Culture medium.Wherein, in the state of culture medium is filled in culture vessel, sterilization processing can be carried out, in this case,
Having the advantages that can be while carries out sterilization treatment to container and culture medium.
Afterwards, Sparassia crispa (Wulf.) Fr. strain (step S140) is inoculated with culture medium.Specifically, hold in the culture filled with culture medium
Inoculation Sparassia crispa (Wulf.) Fr. strain in device.Wherein, the plastic translucent container of 1.1 liters of capacity can be used as culture vessel.
In the case of translucent container, be easy to observation in incubation in the wood flour in the shape of the former base of white states land
The advantage of state.
Like this, after being inoculated with strain, guiding fruiting (step S150) while cultivating strain in culture medium.Wherein,
The condition of culture of 25 DEG C of constant temperature and 70% air humidity can be used, but the present invention is not necessarily limited to this.
In the case of embodiments of the invention, after being inoculated with, through generating in the sawdust medium in container
White former base simultaneously stably carries out period of land.And the time point after inoculation through 1 month, observes on surface
Start to fruiting, sending out for Sparassia crispa (Wulf.) Fr. is realized by the sporophore in flower shape being grown from the upper face of sawdust medium
Educate, after observation fruiting, terminated development through 1 month.As a result, in an embodiment of the present invention, after strain inoculation, about through 2
Individual month, can list as commodity.
Fig. 2 is the figure for illustrating the shape for generating former base in culture vessel by the cultural method of embodiments of the invention.
Fig. 2 is to be inoculated with shape when 20 days after strain, confirms that bacterium is white and states of stably land.
Fig. 3 is the figure of the Sparassia crispa (Wulf.) Fr. for illustrating that embodiments in accordance with the present invention finally grow.As shown in figure 3, in culture
In container after Sparassia crispa (Wulf.) Fr. fruiting, through 1 month, so that it may confirm Sparassia crispa (Wulf.) Fr. and grown into and can list as commodity vigorously
Degree.
In the common Auricularia training method using existing sawdust medium, even if the extra nutritional labeling of injection
(nutrient), cannot also remove the active oxygen that culture medium is accumulated in incubation, therefore until the generation and development of fruiting
Need to consume 3 months degree, wherein including the appearance 2 months of fruiting respectively and growing 1 month.
On the other hand, if using such as the culture for being added with antioxidant, aminoacid and vitamin of embodiments of the invention
Base, then can provide and remove the culture environment of active oxygen to make the stable land of strain be natural, and by guiding fast fast-growing
Length can make the generation time point of fruiting shorten to 1 month.Therefore, in an embodiment of the present invention, as the development of Sparassia crispa (Wulf.) Fr. needs
Including from 1 month required for inoculation strain to fruiting and the time of 2 totally months from 1 month required for fruiting to development,
Therefore the product of high-quality can be produced at short notice in a large number.
In an embodiment of the present invention, antioxidant, aminoacid and vitamin are only used as removing or suppressing in culture
During accumulate on the active oxygen of culture medium, with different category compared with the nutrient for generally being added in existing wood flour
Property, and it is big in the aspect contribution that culture speed can be improved merely with the function that active oxygen is removed in culture medium.At these
In embodiments of the invention, if additionally mixing the battalion added in addition to antioxidant, aminoacid and vitamin in fermentation wood flour
Foster agent, then produce double effectses in the aspect of the supply of the removal and nutritional labeling of active oxygen, therefore can more improve silk ball
The growth efficiency and quality of bacterium.Certainly, in the case of vitamin or aminoacid, not only can remove or inhibitory activity oxygen, and
Nutrient can be played a part of.
Hereinafter, to for derive embodiments of the invention Sparassia crispa (Wulf.) Fr. cultural method and using experimental example illustrate.
In following table 1, the composition and concentration used in each experiment is schematically illustrated.
Table 1
It is independent mixed vitamin or situation during antioxidant in wood flour to illustrate 1,2.3,4 are illustrated in the same scale one
With situation when input antioxidant and vitamin, antioxidant and aminoacid.It is together to put into antioxygen with same ratio to illustrate 5
Situation when agent, aminoacid and vitamin.
Wherein, each illustration is carried out in the way of input concentration is changed to 100ppm, 50ppm, 25ppm and 10ppm
Experiment.Illustratively, illustrating in the case of 1, experiment is subdivided into following situation respectively, i.e. in wood flour, with 100ppm
Concentration input vitamin situation, with the situation of the concentration of 50ppm input vitamin, with the concentration input vitamin of 25ppm
Situation and the situation of vitamin is put into the concentration of 10ppm.Also, prepare 16 culture vessels by each concentration, and it is right
Each concentration has carried out 16 experiments.
Illustrate 4 and illustrate 5 in the case of, experiment be subdivided in wood flour respectively, respectively put into 100ppm, 50ppm,
The antioxidant and aminoacid of 25ppm and 10ppm, or the situation of antioxidant, aminoacid, vitamin.Also press in the case
Each concentration has prepared 16 culture vessels, and 16 experiments have been carried out to each concentration.
Also, in each illustration, 16 cultures for determining in the experiment of each concentration respectively in the same period are held
After the length of the Sparassia crispa (Wulf.) Fr. cultivated in device, the average length of 16 Sparassia crispa (Wulf.) Fr. for determining is calculated, and its result is with reference to following
Table 2.
Table 2
| 100ppm | 50ppm | 25ppm | 10ppm | |
| Illustrate 1 (vitamin) | 3cm | 4.1cm | 6.1cm | 5cm |
| Illustrate 2 (antioxidants) | 4.3cm | 5.7cm | 8.5cm | 7.2cm |
| Illustrate 3 (antioxidants+vitamin) | 5.5cm | 6.9cm | 9.3cm | 8cm |
| Illustrate 4 (antioxidants+aminoacid) | 6.9cm | 8cm | 10.5cm | 9.5cm |
| Illustrate 5 (antioxidants+aminoacid+vitamin) | 7.6cm | 8.7cm | 11.2cm | 10.2cm |
During 5 illustrations altogether can be confirmed in the result of table 2, illustrate 4 and very outstanding result is derived in illustrating 5.That is, may be used
Confirm illustration 5 and remaining example for attempting to be cultivated in the culture medium of antioxidant, aminoacid and vitamin is added with
Show and compare, the speed of growth is most fast.From from the perspective of input concentration, it is known that in each composition, it is 25ppm in input concentration
When it is fitst water, show Seedling height efficiency on the whole in the range of the 10ppm to 25ppm.
According to the Sparassia crispa (Wulf.) Fr. culture culture medium of the antioxidant, aminoacid and vitamin for utilizing the present invention as above
Compound method, has the advantage that, i.e. can utilize and include the culture medium of antioxidant, aminoacid and vitamin to cultivate
Strain, and then the active oxygen that culture medium is accumulated in incubation is removed, so as to the fruiting of funguses can be promoted, when shortening culture
Between, therefore it is individual to produce the high high-quality of commodity value at short notice in batches.
The present invention is illustrated using the embodiment being shown in the drawings as reference, but this is only illustratively, as long as
General technical staff of the technical field of the invention, with regard to it should be understood that various changes and other enforcements of equalization thus can be carried out
Example.Therefore, depending on real technical protection domain of the invention should be according to the technological thought of the claimed scope of invention.
Claims (2)
1. a kind of compound method of Sparassia crispa (Wulf.) Fr. culture culture medium, it is characterised in that
Including：
By in the fermentation wood flour being ready for respectively with 1:1 ratio mixing antioxidant and aminoacid, prepare strain training
Support the step of using culture medium；
The step of sterilization processing is carried out to above-mentioned culture medium；
The step of Sparassia crispa (Wulf.) Fr. strain is inoculated with above-mentioned culture medium；And
The step of culture is inoculated in the above-mentioned strain in above-mentioned culture medium,
In the step of preparing above-mentioned culture medium, melting concn is the above-mentioned anti-of 10 to 25ppm respectively in the above-mentioned fermentation wood flour
Oxidant and above-mentioned aminoacid,
Above-mentioned antioxidant is glutathion, and above-mentioned aminoacid is histidine.
2. a kind of compound method of Sparassia crispa (Wulf.) Fr. culture culture medium, it is characterised in that
Including：
By in the fermentation wood flour being ready for respectively with 1:1:1 ratio mixes antioxidant, aminoacid and vitamin, makes
The step of standby spawn culture culture medium；
The step of sterilization processing is carried out to above-mentioned culture medium；
The step of Sparassia crispa (Wulf.) Fr. strain is inoculated with above-mentioned culture medium；And
The step of culture is inoculated in the above-mentioned strain in above-mentioned culture medium,
In the step of preparing above-mentioned culture medium, melting concn is the above-mentioned anti-of 10 to 25ppm respectively in the above-mentioned fermentation wood flour
Oxidant, above-mentioned aminoacid and said vitamin,
Above-mentioned antioxidant is glutathion, and above-mentioned aminoacid is histidine, and said vitamin is ascorbic acid or vitamin C.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150130462A KR101599699B1 (en) | 2015-09-15 | 2015-09-15 | Method for manufacturing culture medium of sparassis crispa using antioxidantm, amino acid and vitamin |
| KR1020150130461A KR101599697B1 (en) | 2015-09-15 | 2015-09-15 | Method for manufacturing culture medium of sparassis crispa using antioxidant and amino acid |
| KR10-2015-0130461 | 2015-09-15 | ||
| KR10-2015-0130462 | 2015-09-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106520561A true CN106520561A (en) | 2017-03-22 |
Family
ID=58343515
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610807503.9A Pending CN106520561A (en) | 2015-09-15 | 2016-09-05 | Method for manufacturing culture medium of sparassis crispa |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106520561A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006063116A2 (en) * | 2004-12-09 | 2006-06-15 | Griffith Owen W | Bifunctional enzyme with ϝ-glutamyleysteine synthetase and glutathione synthetase activity and uses thereof |
| CN1823571A (en) * | 2006-03-24 | 2006-08-30 | 张晓明 | Artificial cultivation method of hydranginic bacteria |
| CN101955392A (en) * | 2010-09-19 | 2011-01-26 | 福建省农业科学院食用菌研究所 | Formula of culture medium for industrial production of sparasis crispa and production process |
| CN103733882A (en) * | 2013-12-28 | 2014-04-23 | 朱斗锡 | Novel method for producing sparassis crispa in factorization mode |
-
2016
- 2016-09-05 CN CN201610807503.9A patent/CN106520561A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006063116A2 (en) * | 2004-12-09 | 2006-06-15 | Griffith Owen W | Bifunctional enzyme with ϝ-glutamyleysteine synthetase and glutathione synthetase activity and uses thereof |
| CN1823571A (en) * | 2006-03-24 | 2006-08-30 | 张晓明 | Artificial cultivation method of hydranginic bacteria |
| CN101955392A (en) * | 2010-09-19 | 2011-01-26 | 福建省农业科学院食用菌研究所 | Formula of culture medium for industrial production of sparasis crispa and production process |
| CN103733882A (en) * | 2013-12-28 | 2014-04-23 | 朱斗锡 | Novel method for producing sparassis crispa in factorization mode |
Non-Patent Citations (3)
| Title |
|---|
| 周铭锋: "响应面法优化谷胱甘肽发酵培养基的研究", 《食品与发酵科技》 * |
| 张昌伟 等: "响应面法优化L-组氨酸发酵培养基", 《发酵科技通讯》 * |
| 陈士瑜: "《菌菇生产技术全书》", 31 December 1999 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103891524B (en) | The method of glossy ganoderma dish garden formula cultivation and the medium for cultivating ganoderma | |
| CN105284520A (en) | Method promoting fig cuttage rooting | |
| CN103120126B (en) | Method for carrying out tissue cultivation propagation of anoectochilus roxburghii by intermittent submerged bioreactor | |
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN101874507A (en) | Microbioecological formulation for blueberry cultivation and planting, preparation method and use method thereof | |
| CN104541977A (en) | Chinese fir board cultivating method of antrodia cinnamomea | |
| KR100483333B1 (en) | Production method of the cauliflower mushroom using fermented sawdust | |
| CN105884464A (en) | Edible fungus cultivation matrix and manufacturing method thereof | |
| CN108029458A (en) | A kind of preparation method of Termitomyces albuminosus with black skin liquid spawn | |
| CN107750814A (en) | A kind of breeding method of Wild ganoderma strain | |
| CN107629966A (en) | Fungal elicitor, its preparation method and the method that bletilla striata quick reproduction technique is carried out using the fungal elicitor | |
| CN107125017A (en) | A kind of breeding method of mushroom | |
| CN109089466B (en) | Method for promoting celery seed germination | |
| CN107278562A (en) | A kind of cuttage and seedling culture method of tea tree | |
| CN107125024A (en) | A kind of cultural method for improving black fungus quality | |
| KR101401791B1 (en) | Cultivation method of shiitake mushrooms | |
| US1832593A (en) | Process for the cultivation of edible fungi | |
| CN109182144A (en) | The fungal elicitor and the preparation method and application thereof for promoting purple dendrobium seed to sprout | |
| CN106520561A (en) | Method for manufacturing culture medium of sparassis crispa | |
| CN107996402A (en) | A kind of method for preventing tobacco Brown | |
| CN107950303A (en) | A kind of cultural method of Termitomyces albuminosus with black skin parent species | |
| KR101634857B1 (en) | Cultivation method of shiitake mushrooms | |
| KR101599699B1 (en) | Method for manufacturing culture medium of sparassis crispa using antioxidantm, amino acid and vitamin | |
| KR101373154B1 (en) | Disposable sawdust medium and its manufacturing method | |
| CN105255736A (en) | Preservation method of hypsizigus marmoreus strain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170322 |
|
| WD01 | Invention patent application deemed withdrawn after publication |