KR101599697B1

KR101599697B1 - Method for manufacturing culture medium of sparassis crispa using antioxidant and amino acid

Info

Publication number: KR101599697B1
Application number: KR1020150130461A
Authority: KR
Inventors: 이성우; 조규원; 양경수
Original assignee: 주식회사 일심바이오
Priority date: 2015-09-15
Filing date: 2015-09-15
Publication date: 2016-03-04

Abstract

The present invention relates to a method for preparing a culture medium for cultivating a mushroom of Brassica juncea using an antioxidant and an amino acid. According to the present invention, there is provided a method for producing a fermented sausage, comprising the steps of: preparing a fermented sawdust; mixing an antioxidant and an amino acid in the fermented sawdust respectively to produce a medium for cultivation of the seed culture; sterilizing the medium; And a step of culturing the seeds inoculated in the culture medium.
According to the method for preparing the culture medium for the growth of the mushroom with the antioxidant and the amino acid, the microorganism is cultured using the medium containing the antioxidant and the amino acid, thereby removing the active oxygen accumulated in the medium during the cultivation, And shortening the incubation period, so that it is possible to mass-produce a high quality object having a high commercial value in a short period of time.

Description

TECHNICAL FIELD The present invention relates to a method for preparing a culture medium for cultivating a mushroom of Brassica juncea using an antioxidant and an amino acid,

More particularly, the present invention relates to an antioxidant and an amino acid-blended chrysanthemum which are capable of shortening a culture period by removing active oxygen accumulated in a medium during culturing, And a method for preparing a culture medium for mushroom culture.

Sparassis crispa Wulf.Fr. Is a mushroom of mushroom, bark, mushroom and mushroom. The fruiting bodies of mushrooms have a flower shape that waved white, and they bloom around the roots of living trees, dead stalks and stumps between summer and fall, and live infertility.

It is used for food because it is strong in flesh and texture, and it is widely used for medicinal purposes because various functionalities are recognized. Currently, most of them are produced through indoor artificial cultivation.

Conventionally, a bottle cultivation method using sawdust is mainly used, but it takes a long period of culture, it is difficult to mass-produce, and the yield is low. In addition, nutrients are added to increase the growth efficiency during the cultivation process, but there is a limit to shortening the cultivation period.

A technique which is a background of the present invention is disclosed in Korean Patent No. 1385540 (published on Apr. 14, 2014).

It is an object of the present invention to provide a method for preparing a culture medium for cultivation of Rosemary mushroom using an antioxidant and an amino acid, which is capable of inhibiting aging and promoting growth by shortening the incubation period by providing a culture environment free of active oxygen.

The present invention relates to a method of preparing a culture medium for cultivating a mushroom of Brassica juncea, which is capable of shortening a culture period by removing active oxygen which may occur during culture in a limited container, comprising the steps of mixing an antioxidant and an amino acid in the prepared fermented sawdust, A step of sterilizing the culture medium, a step of inoculating a bacterium of the genus Rosa mushroom in the culture medium, and a step of culturing the seed bacterium inoculated in the culture medium, do.

Here, in the step of preparing the medium, the antioxidant and the amino acid may be mixed at a ratio of 1: 1 in the fermented sawdust.

In addition, the step of producing the medium may comprise mixing the antioxidant and the amino acid in a concentration of 10 to 25 ppm in the fermented sawdust, respectively, wherein the antioxidant may be glutathione and the amino acid is histidine ).

According to the method for preparing a culture medium for cultivating the mushroom, the antioxidant and the amino acid according to the present invention are used for culturing a seed culture using a medium containing an antioxidant and an amino acid, thereby removing active oxygen accumulated in the medium during the cultivation, And the culture period is shortened, so that it is possible to mass-produce a high quality object having a high commercial value in a short period of time.

FIG. 1 is a flow chart of a method for cultivating a mushroom cultivar using an antioxidant and an amino acid according to an embodiment of the present invention.
FIG. 2 is a view showing a state in which a refreshing agent is generated in a culture container through a culture method according to an embodiment of the present invention.
FIG. 3 is a graph showing the final growth of the mushroom in accordance with the embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention.

The present invention relates to a method for preparing a culture medium for cultivating a mushroom of Brassica juncea using an antioxidant and an amino acid. The embodiment of the present invention can increase the growth efficiency and shorten the cultivation period by cultivating the seeds in the medium environment in which the active oxygen is removed, and can produce a large amount of objects with a high value for a short period of time.

Hereinafter, a method for cultivating the mushroom of the present invention will be described in detail. FIG. 1 is a flow chart of a method for cultivating a mushroom cultivar using an antioxidant and an amino acid according to an embodiment of the present invention.

First, fermented sawdust for preparing a medium is prepared (S110).

Here, the fermented sawdust may be a fermented larch sawdust of a conventional one. Specifically, phenol compounds are removed from larch sawdust and used for non-fermentation for 6 months for fiber softening. The prepared fermented sawdust can be adjusted to a water content of 60 to 70% (ex, 65%).

Next, the fermented sawdust is mixed with an antioxidant and an amino acid, respectively, to prepare a culture medium for seed culture (S120). Here, glutathione may be used as the antioxidant component, and histidine (ex, L type histidine) may be used as the amino acid component.

In addition, in the medium preparation step, antioxidant (glutathione) and amino acid (histidine) may be added and mixed at a ratio of 1: 1 (weight ratio) in the fermented sawdust. Since the feed ratio between the added substances is the same, the production of the medium is easy.

However, it is preferable to use all of the antioxidant and amino acid having a concentration in the range of 10 to 25 ppm. The antioxidant and amino acid injected here may mean that they are diluted in a solvent such as water to the corresponding concentration. In the examples of the present invention, when the concentration of the antioxidant and the amino acid is less than 10 ppm or more than 25 ppm, the growth rate is slow and the growth length is short and the production efficiency is low. This will be explained in detail through the experimental results.

Thus, when a medium containing an antioxidant and an amino acid is used, it is possible to effectively remove active oxygen which accumulates in the medium during the culturing process and acts as a cause of aging, thereby improving the foot or growth environment, It can speed up.

Next, the produced medium is sterilized (S130). In the sterilization treatment, the medium is sterilized by steam sterilization in a sterilization pot. This can remove harmful bacteria and produce sterile media. Here, the sterilization treatment can be performed in the state that the culture medium is filled in the culture medium. In this case, there is an advantage that the sterilization of the culture medium and the medium can be performed at the same time.

Thereafter, the mushroom seedlings are inoculated in the medium (S140). Specifically, the seedling of the mushroom is inoculated into a culture container filled with a medium. Here, as the culture container, a translucent container made of plastic having a capacity of 1.1 liters can be used. In the case of translucent containers, it is easy to observe the state of the flasks that are settled white in the sawdust during the culturing process.

After the inoculation of the seeds, the foot is induced while culturing the seeds in the medium (S150). Here, the culture conditions may be a constant temperature of 25 DEG C and an air humidity of 70%, but the present invention is not necessarily limited thereto.

In the case of the embodiment of the present invention, after the inoculation, white streaks are produced in the sawdust medium in the container, and the streaks are stably placed. After one month from the inoculation, the feet are observed on the surface, and the flower-shaped fruiting body is raised on the upper surface of the sawdust medium, and the growth of the mushroom is accomplished. When the feet are observed for about one month after the observation, the growth is completed . As a result, the embodiment of the present invention can be shipped as a product for about two months after seedling inoculation.

FIG. 2 is a view showing a state in which a refreshing agent is generated in a culture container through a culture method according to an embodiment of the present invention. Fig. 2 shows that the bacteria appeared stable for 20 days after inoculation.

FIG. 3 is a graph showing the final growth of the mushroom in accordance with the embodiment of the present invention. After about one month after the mushroom emerged in the culture vessel, it can be confirmed that the mushroom was grown vigorously to the extent that it can be dispensed as a product as shown in Fig.

Since the conventional mushroom culture method using the conventional sawdust medium can not remove the active oxygen accumulated in the culture medium during the culturing process even when the nutrient (nutrient) is injected, the development and growth of the foot including 2 months and 1 month It takes about three months.

On the other hand, the use of a medium supplemented with an antioxidant and an amino acid as in the example of the present invention provides a culture environment in which active oxygen is removed, thereby enabling stable deposition of seeds. Month. Therefore, the embodiment of the present invention requires a period of two months including one month from the inoculation of the seedling to one foot and one month until the growth, so that a high-quality product can be mass-produced in a short period of time.

In the examples of the present invention, the antioxidant and the amino acid are used to remove or inhibit the active oxygen accumulated in the medium only during the culturing process. The antioxidant and the amino acid have different properties from the nutrients generally added to the sawdust, But also contributes to the increase of the cultivation speed by the function of removing only. In this embodiment of the present invention, in addition to an antioxidant and an amino acid, if additional nutrients are separately mixed in the fermented sawdust, a double effect of removal of active oxygen and feeding of nutrients may occur, thereby further enhancing the growth efficiency and quality of mushroom. Amino acids, of course, can act as nutrients as well as inhibit or inhibit free radicals.

Hereinafter, experimental examples used to derive the method for cultivating the mushroom of the present invention will be described. Table 1 below summarizes the components and concentrations used in each experiment.

Ingredients added to sawdust Type of input concentration Example 1 Vitamin (ascorbic acid) 100, 50, 25, 10 ppm Example 2 Antioxidant (glutathione) 100, 50, 25, 10 ppm Example 3 Antioxidant (glutathione) + vitamin (ascorbic acid) 100, 50, 25, 10 ppm Example 4 Antioxidant (glutathione) + amino acid (histidine) 100, 50, 25, 10 ppm

Examples 1 and 2 are cases in which vitamins or antioxidants are mixed singly in sawdust. Examples 3 and 4 are cases where antioxidants, vitamins, antioxidants and amino acids are added together at the same ratio.

Here, experiments were carried out while changing the input concentrations to 100, 50, 25, and 10 ppm for each example. As an example, the case of Example 1 is subdivided into experiments in the case of feeding 100 ppm of vitamins into sawdust, 50 ppm of concentration, 25 ppm of concentration, and 10 ppm of concentration. In addition, 16 culture vessels were prepared for each concentration and 16 experiments were performed.

In the case of Example 4 as well, it is further divided into experiments in which 100 ppm of antioxidant and amino acid are added to sawdust, 50 ppm is added, 25 ppm is added, and 10 ppm is added. In this case, 16 culture vessels were prepared for each concentration and 16 experiments were performed.

In each example, the length of the mushroom cultivated in 16 culture vessels was measured for each concentration experiment, and the average of the 16 measured lengths was calculated. The results are shown in Table 2 below.

100ppm 50 ppm 25 ppm 10ppm Example 1 (Vitamin) 3cm 4.1cm 6.1cm 5cm Example 2 (Antioxidant) 4.3cm 5.7cm 8.5cm 7.2cm Example 3 (Antioxidant + Vitamin) 5.5cm 6.9 cm 9.3cm 8cm Example 4 (antioxidant + amino acid) 6.9 cm 8cm 10.5cm 9.5cm

From the results of Table 2, it can be seen that the best results of Example 4 are derived from the four examples. That is, in Example 4 in which the culture was carried out in a medium supplemented with both an antioxidant and an amino acid, the growth rate was the fastest in comparison with the other examples. From the viewpoint of the feed concentration, it was found that the feed concentration of each component was the best when the feed concentration was 25 ppm, and the growth efficiency was high in the range of 10 to 25 ppm as a whole.

According to the method for preparing a culture medium for cultivating a mushroom of Brassica juncea using the antioxidant and amino acid according to the present invention, by cultivating a seed bacterium using an antioxidant and an amino acid-containing medium, Thereby promoting the foot of the mushroom and shortening the culture period, thereby enabling the mass production of a high quality object having a high commercial value in a short period of time.

While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims

Mixing the antioxidant and the amino acid in a ratio of 1: 1 in the prepared fermentation sawdust to prepare a culture medium for seed culture;
Sterilizing the medium;
A step of inoculating a bryophyta mushroom strain in the medium; And
Culturing said seeds inoculated in said medium,
The step of preparing the medium comprises:
Mixing the fermented sawdust with the antioxidant and the amino acid at a concentration of 10 to 25 ppm,
Wherein the antioxidant is glutathione and the amino acid is histidine.

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