CN106511370A - Bacteriostatic liquid - Google Patents
Bacteriostatic liquid Download PDFInfo
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- CN106511370A CN106511370A CN201610946256.0A CN201610946256A CN106511370A CN 106511370 A CN106511370 A CN 106511370A CN 201610946256 A CN201610946256 A CN 201610946256A CN 106511370 A CN106511370 A CN 106511370A
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- carboxymethyl chitosan
- average molecular
- molecular weight
- antibacterial liquid
- carboxymethyl
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/716—Glucans
- A61K31/722—Chitin, chitosan
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
Abstract
The invention discloses bacteriostatic liquid. The bacteriostatic liquid is an aqueous solution containing carboxymethyl chitosan, and the number-average molecular weight of carboxymethyl chitosan is 1000-2000. A test shows that carboxymethyl chitosan in the bacteriostatic liquid has not only the known anti-bacterial function but also an antiallergic function so that carboxymethyl chitosan can be directly prepared to or be mixed with other annexing agents to be prepared to various kinds of bacteriostatic liquid and various kinds of dosage forms to be used for non-woven fabrics. The bacteriostatic liquid is not only convenient to use but also good in bacteriostatic and antiallergic effect, is colorless and tasteless, and can be effectively used in the personal hygiene nursing materials such as disposable diapers used by infants and old people, sanitary towels used by women and non-woven fabrics used in non-woven fabric wet paper towels. Red and swollen and pruritic skin problems appearing in the use process of a user are avoided.
Description
Technical field
The present invention relates to a kind of antibacterial liquid, and in particular to a kind of antibacterial liquid for non-woven fabrics.
Background technology
Diaper becomes one of baby and old man's necessary, if but existing diaper change not in time when in use
Or as the skin that heat is easily caused baby plays erythema or chafing, very big wound is brought to baby
Evil.In order to solve this problem, some technical schemes in prior art, are provided.
Domestic applications number disclose a kind of Chinese medicine composition for diaper for the patent of invention of 201510143244.X
And the preparation method of diaper, wherein the Chinese medicine composition, by Flos Lonicerae, the Rhizoma Atractylodis Macrocephalae, Poria, Rhizomadioscoreae, Semen Coicis, Fructus Atriplicis Sibiricae,
Herba Menthae, Cortex Dictamni, Cortex Phellodendri, Herba Taraxaci, Cortex Moutan, Radix Saposhnikoviae composition；Above-mentioned Chinese medicine composition is cleaned up and added and is soaked, and
Decoct the nonwoven cloth cover that diaper is infiltrated after obtaining medicinal liquid.During the traditional Chinese medical science is combined in this invention, various medical material effects use it for paper
The direct skin contact active ingredient of user buttocks can be caused in urine pants, the effect of prevention and treatment one is reached.It is domestic
The patent of invention of Application No. 2015107034304 disclose a kind of bacteriostatic solution and its made by diaper and diaper
Preparation method, this bacteriostatic solution, which is made up of components such as following mass parts, Hard Fat alcohol ether -21, hard alcohol ethers -2, will be this
Bacteriostatic solution is used for the surface layer of non-woven fabrics and plays pro-skin and antibacterial effect.
But it is complicated using preparation process during Chinese medicine ingredients, and Chinese medicine typically can be flavoursome, not by last point of market
Consumers welcomed；Using the antibacterial liquid in documents 2, its antibacterial effect has, but not antiallergic effect, and user exists
Remain on during use and skin problem easily occur.
The content of the invention
The shortcoming of prior art in view of the above, it is an object of the invention to provide a kind of for the antibacterial of non-woven fabrics
Liquid.
To achieve these goals or other purposes, the present invention is achieved by the following technical solutions.
A kind of antibacterial liquid for non-woven fabrics, described antibacterial liquid is the aqueous solution containing carboxymethyl chitosan, the carboxylic
The number-average molecular weight of methyl chitosan is 1000～20000.
Preferably, the concentration of the carboxymethyl chitosan sugar aqueous solution is not less than 0.1wt%.It is highly preferred that the carboxylic first
The concentration of base enclosure water solution is not less than 0.2wt%.It is highly preferred that the concentration of the carboxymethyl chitosan sugar aqueous solution is not for
Less than 1.0wt%.
Heretofore described number-average molecular weight be according to《Pharmacopoeia of People's Republic of China (four)》(version in 2015) general rule
0401 " ultraviolet visible spectrophotometry " method determines A525.Its principle is：Acetylacetone,2,4-pentanedione reagent and standard glucosamine, carboxylic
The reducing end group reaction of methyl chitosan or its hydrolyzed solution produces chromogen；Under certain condition, absorbance A525With it is corresponding
The molar concentration of sugar has linear relationship.The multiple of the reducing end group after hydrolysis than increasing before hydrolysis is exactly the de- second of the carboxymethyl
The average degree of polymerization n of acyl chitin, in binding molecule, sugar unit average molecular mass can calculate the equal molecule matter of number of the sugar
Amount.
It is highly preferred that the carboxymethyl chitosan is O- carboxymethylchitosans.The deacetylated shell of the O- carboxymethyls
Polysaccharide is that the 6- hydroxyl hydrogens of the deacetylated rear or shitosan of carboxymethyl chitin are carboxymethylated by the part after carboxymethyl replacement
Chitosan.
More specifically, the structural formula of the O- carboxymethylchitosans is as follows：
Wherein R1For CH2COONa；R2For CH2COONa or H；R3For CH2COONa or H or COCH3。
Preferably, the preparation method of the carboxymethyl chitosan is included number-average molecular weight higher carboxymethyl chitosan glycogen
Material is degraded by hydrogen peroxide.It is further preferable that carboxymethyl chitosan in the higher carboxymethyl chitosan raw material of the number-average molecular weight
The number-average molecular weight 30～700,000 of sugar.It is further preferable that carboxylic first in the higher carboxymethyl chitosan raw material of the number-average molecular weight
Base substitution value is 0.8～1.3.It is further preferable that carboxymethyl O positions in the higher carboxymethyl chitosan raw material of the number-average molecular weight
Substitution value is that 0.7～1.0, N positions substitution value is less than 0.3.
The higher carboxymethyl chitosan raw material of the number-average molecular weight be by domestic applications number for 2013105362023 send out
Method disclosed in bright patent is prepared, and it discloses a kind of method for preparing carboxymethyl chitosan, and described method is with first
Shell element is raw material, and first chitin alkalizes, and carries out deacetylation using the method for colding and heat succeed each other, and produces shitosan sodium salt, then
Carboxylation reaction is carried out under alkalescence condition, the higher carboxymethyl chitosan sugar product of number-average molecular weight is obtained.The letter of the inventive method technique
It is single and easily controllable, it is to avoid because long term high temperature reacts the chain cleavage for occurring during chitosan molecule chain deacetylation, from
And the more preferable carboxymethyl chitosan of a kind of high viscosity, high substituted degree and water solublity is obtained, it is adapted to large-scale industrial production.Institute
The pH scopes for stating the higher carboxymethyl chitosan dissolving of molecular weight are 4.5～14.0.The higher carboxylic first of described number-average molecular weight
Base enclosure polysaccharide is soluble in the acid solution that pH is 4.5～6.5.The carboxymethyl chitosan dissolving obtained with traditional handicraft
PH scopes are compared for 7.0～14.0, the pH of the higher carboxymethyl chitosan dissolving of the number-average molecular weight that above-mentioned patented method is obtained
Scope is wider, has widened its application.
Preferably, the technique of hydrogen peroxide degraded is：The carboxylic for adding number-average molecular weight higher under agitation hydrogen peroxide
Degradation reaction is carried out in methyl chitosan, it is 8.0～8.5 to add alkali to cause the pH of reaction system during degradation reaction, until anti-
The viscosity for answering system adds sodium sulfite stopped reaction when being 80～120cp.It is highly preferred that in degradation reaction, reaction temperature is
25～45 DEG C.Carboxymethyl chitosan after being degraded by above-mentioned degradation reaction.
It is highly preferred that the carboxymethyl chitosan after the preparation method of the carboxymethyl chitosan includes degrading hydrogen peroxide enters
Row acid out is washed.The acid out washing refers to that be 6～7 to pH with the carboxymethyl chitosan sugar material body after acid adjustment hydrogen peroxide degraded,
Add ethanol to separate out to light yellow crystal, then use ethanol cyclic washing, dry, be dried and obtain carboxymethyl chitosan.
The invention discloses a kind of purposes of antibacterial liquid as described above on sanitary care article is prepared.
Preferably, the Personal hygiene nursing materialss include nonwoven layer, and the nonwoven layer is using as described above
Antibacterial liquid forms antibacterial layer.
Preferably, the nonwoven layer on Personal hygiene nursing materialss includes diaper for baby and old man, women
The nonwoven layer used on sanitary towel, wet nonwoven napkin and facial film.
The invention also discloses a kind of Personal hygiene nursing materialss, the Personal hygiene nursing materialss include nonwoven layer,
The nonwoven layer forms antibacterial layer using antibacterial liquid described above.Test shows, carboxymethyl shell described above in the present invention
It with antianaphylactic effect, can be mixed system directly or with other additives in addition to known antibacterial functions by polysaccharide
Into various antibacterial liquids and various dosage forms, for non-woven fabrics.Its not only convenient use, and antibacterial antiallergic effect is good, and
Colorless and odorless, the skin problem that non-woven fabrics user effectively can be avoided to occur in use.
Specific embodiment
Embodiments of the present invention are illustrated below by way of specific instantiation, those skilled in the art can be by this specification
Disclosed content understands other advantages and effect of the present invention easily.The present invention can also pass through concrete realities different in addition
The mode of applying is carried out or applies, the every details in this specification can also based on different viewpoints with application, without departing from
Various modifications and changes are carried out under the spirit of the present invention.
Before the specific embodiment of the invention is further described, it should be appreciated that protection scope of the present invention is not limited to down
State specific specific embodiment；It is also understood that the term used in the embodiment of the present invention is specific concrete in order to describe
Embodiment, rather than in order to limit the scope of the invention.The test method of unreceipted actual conditions in the following example,
Generally according to normal condition, or according to the condition proposed by each manufacturer.
When embodiment provides numerical range, it should be appreciated that except non-invention is otherwise noted, two ends of each numerical range
Between point and two end points, any one numerical value can select.Unless otherwise defined, the present invention used in all technologies and
The same meaning that scientific terminology is generally understood that with those skilled in the art of the present technique.Except the concrete grammar used in embodiment, equipment,
Outside material, the record of grasp and the present invention according to those skilled in the art to prior art can also be used and this
Any method of the similar or equivalent prior art of method, equipment described in inventive embodiments, material, equipment and material come real
The existing present invention.
Embodiment 1
It is to prepare the carboxymethyl chitosan that number-average molecular weight is 2000 in the present embodiment.
Degraded with hydrogen peroxide using the higher carboxymethyl chitosan raw material of number-average molecular weight, the number-average molecular weight is higher
The number-average molecular weight of carboxymethyl chitosan raw material is 30W, degree of substitution by carboxymethyl is 0.8～1.3, wherein, O is degree of substitution by carboxymethyl
0.3 is less than for 0.7～1.0, N positions substitution value.
Hydrogen peroxide degraded technique be：The carboxymethyl chitosan for adding number-average molecular weight higher under agitation hydrogen peroxide
Degradation reaction is carried out in sugar, it is 8.0 to add alkali to cause the pH of reaction system during degradation reaction, until the viscosity of reaction system is
Sodium sulfite stopped reaction is added during 80cp.In degradation reaction, reaction temperature is room temperature.
The preparation method of the carboxymethyl chitosan includes that carrying out acid out to the carboxymethyl chitosan after hydrogen peroxide degraded washes
Wash.Acid out washing refers to that be 7 to pH with the carboxymethyl chitosan sugar material body after acid adjustment hydrogen peroxide degraded, adds ethanol to light
Yellow crystals are separated out, then use ethanol cyclic washing, are dried, and are dried and are obtained carboxymethyl chitosan.
Embodiment 2
Number-average molecular weight is adopted in the present embodiment for 8000 carboxymethyl chitosan.
Degraded with hydrogen peroxide using the higher carboxymethyl chitosan raw material of number-average molecular weight, the number-average molecular weight is higher
The number-average molecular weight of carboxymethyl chitosan raw material is 40W, degree of substitution by carboxymethyl is 0.8～1.3, wherein, O is degree of substitution by carboxymethyl
0.3 is less than for 0.7～1.0, N positions substitution value.
Hydrogen peroxide degraded technique be：The carboxymethyl chitosan for adding number-average molecular weight higher under agitation hydrogen peroxide
Degradation reaction is carried out in sugar, it is 8.2 to add alkali to cause the pH of reaction system during degradation reaction, until the viscosity of reaction system is
Sodium sulfite stopped reaction is added during 80cp.In degradation reaction, reaction temperature is room temperature.
The preparation method of the carboxymethyl chitosan includes that carrying out acid out to the carboxymethyl chitosan after hydrogen peroxide degraded washes
Wash.Acid out washing refers to that be 6 to pH with the carboxymethyl chitosan sugar material body after acid adjustment hydrogen peroxide degraded, adds ethanol to light
Yellow crystals are separated out, then use ethanol cyclic washing, are dried, and are dried and are obtained carboxymethyl chitosan.
Embodiment 3
Number-average molecular weight is adopted in the present embodiment for 15000 carboxymethyl chitosan.
Degraded with hydrogen peroxide using the higher carboxymethyl chitosan raw material of number-average molecular weight, the number-average molecular weight is higher
The number-average molecular weight of carboxymethyl chitosan raw material is 55W, degree of substitution by carboxymethyl is 0.8～1.3, wherein, O is degree of substitution by carboxymethyl
0.3 is less than for 0.7～1.0, N positions substitution value.
Hydrogen peroxide degraded technique be：The carboxymethyl chitosan for adding number-average molecular weight higher under agitation hydrogen peroxide
Degradation reaction is carried out in sugar, it is 8.2 to add alkali to cause the pH of reaction system during degradation reaction, until the viscosity of reaction system is
Sodium sulfite stopped reaction is added during 120cp.In degradation reaction, reaction temperature is room temperature.
The preparation method of the carboxymethyl chitosan includes that carrying out acid out to the carboxymethyl chitosan after hydrogen peroxide degraded washes
Wash.Acid out washing refers to that be 6 to pH with the carboxymethyl chitosan sugar material body after acid adjustment hydrogen peroxide degraded, adds ethanol to light
Yellow crystals are separated out, then use ethanol cyclic washing, are dried, and are dried and are obtained carboxymethyl chitosan.
Embodiment 4
Number-average molecular weight is adopted in the present embodiment for 20000 carboxymethyl chitosan.
Degraded with hydrogen peroxide using the higher carboxymethyl chitosan raw material of number-average molecular weight, the number-average molecular weight is higher
The number-average molecular weight of carboxymethyl chitosan raw material is 70W, degree of substitution by carboxymethyl is 0.8～1.3, wherein, O is degree of substitution by carboxymethyl
0.3 is less than for 0.7～1.0, N positions substitution value.
Hydrogen peroxide degraded technique be：The carboxymethyl chitosan for adding number-average molecular weight higher under agitation hydrogen peroxide
Degradation reaction is carried out in sugar, it is 8.4 to add alkali to cause the pH of reaction system during degradation reaction, until the viscosity of reaction system is
Sodium sulfite stopped reaction is added during 100cp.In degradation reaction, reaction temperature is room temperature.
The preparation method of the carboxymethyl chitosan includes that carrying out acid out to the carboxymethyl chitosan after hydrogen peroxide degraded washes
Wash.The acid out washing refers to that be 6.5 to pH with the carboxymethyl chitosan sugar material body after acid adjustment hydrogen peroxide degraded, adds ethanol extremely
Light yellow crystal is separated out, then uses ethanol cyclic washing, is dried, and is dried and is obtained carboxymethyl chitosan.
First, animal experiment
Using the carboxymethyl chitosan prepared in embodiment 1～4 as test specimen, skin resistance state animal experiment is carried out.
That is animal Jing 2, after 4 one dinitrochlorobenzene (DNCB) skin Induction exposure, viewing test sample is to the anti-metamorphosises of skin.Specifically
Ground is：By guinea pig skin Jing after DNCB skin Induction exposures, abnormal (allergy) state is produced, while test specimen is smeared, Jing
Excite contact, the anti-metamorphosises of viewing test low-molecular-weight -90 pairs of guinea pig skins of carboxymethyl chitosan.
1. test material：
Test specimen：1. low-molecular-weight carboxymethyl chitosan in embodiment 1～4
2. 2,4 one dinitrochlorobenzene (positive control)：2nd, 4 one dinitrochlorobenzene (DNCB) are made into 1% with medical ventolin
DNCB vaseline ointment is used for Induction exposure, is made into 0.5%DNCB vaseline ointment for exciting contact to use.
3. compound dexamethason acetate emulsifiable paste：(sample controls) China Resources three nine-day periods after the winter solstice medicine 1504042H
Experimental animal：Albino guinea-pig 30, half and half body weight of male and female：250g-300g
Animal uses credit number for the court：SYXK (Shanghai) 2014-0018
Shanghai Jia Gan bio tech ltd production licence number：SCXK (Shanghai) 2010-0028
2. experimental condition：
20 DEG C ± 2 DEG C of wet temp of test, relative humidity 60～70%
3. test method：
Healthy albino guinea-pig 30 is chosen, the test skin for shaving off two side 8cm of spinal column × 4cm scopes is standby by hair, will be dynamic
Thing is divided into test specimen group, positive controls and blank control group totally 3 groups, per group of 10 animals.
(1) Induction exposure：By 1%DNCB vaseline ointment 0.2g or so, it is coated in test specimen group and positive controls is dynamic
The left side of thing, then be in kind repeated once in 7d and 14d.
(2) smear trial target：Smear in Induction exposure while, with same method, test specimen is applied to into sample sets and is moved
The right side of thing, once a day, continuously smears 28 days.
(3) excite contact：14d after last induction, by the 0.5%DNCB vaseline ointment of about 0.2g, is applied to the dynamic of each group
The right side trial zone of thing, the reaction of trial zone skin after observation contact 24h.
4. judgment criteria：
When it is >=1 that skin erythema response value occurs in test group animal, it is judged to the animal and skin metamorphosis positive reaction occurs.
5. result of the test：
Carboxymethyl chitosan skin excites skin allergic reaction value after contact 24h
(0) it is positive reaction without severe erythema (1) in erythema (1) slight erythema (2) substantially erythema (3).
Experimental concentration：1.0%, refer to the carboxymethyl chitosan sugar aqueous solution using 1.0wt%.
6. result of the test is passed judgment on：
Carboxymethyl chitose in embodiment 1～4 has the anti-metamorphosises of alleviation to white guinea pig skin.
2nd, human experimentation
Using the carboxymethyl chitosan prepared in embodiment 1～4 as test specimen, human experimentation, specially skin are carried out
Acupuncture is tested, and scheme is as described below.
Location parameter：
1 red swelling of the skin degree：Visual score is (from 5 to 1；From being seriously changed into not serious)
2 gargalesthesias：Subjects subjective is felt (from 5 to 1；From being seriously changed into not serious)
The reduction of 3 vesicle areas：Planimetric method
With specific test blade at tuerculoderma position Deca histamine hydrochloride (10mg/ml)；
After 5 minutes, skin can produce serious vesicle and gargalesthesia；
Using the aqueous solution 2ml of the carboxymethyl chitosan of low-molecular-weight in the embodiment of the present invention 1～4, the low-molecular-weight
Carboxymethyl chitosan aqueous solution concentration be 0.2wt%.
Using the carboxymethyl chitosan of the carboxymethyl chitosan of low-molecular-weight in embodiment 1 formation 0.2wt% soluble in water
Aqueous solution, taking 2ml carries out human experimentation, and behind 0,3,30,60.90,120,150 minutes after application, determination test position itches
Sense, red and swollen degree and vesicle area are shown in Table 1：
Table 1
| Time (min) | 0 | 3 | 30 | 60 | 90 | 120 | 150 |
| Red and swollen (positive) | 3.5 | 3.5 | 3.4 | 2.8 | 2.2 | 0.9 | 0.6 |
| Red and swollen (sample) | 3.2 | 3.0 | 2.7 | 1.4 | 0.9 | 0.5 | 0.2 |
| Gargalesthesia (positive) | 3.8 | 3.8 | 3.6 | 2.7 | 1.3 | 1 | 0.4 |
| Gargalesthesia (sample) | 3.9 | 1.6 | 1.4 | 0.9 | 0.3 | 0 | 0 |
| Vesicle area (positive control) | 100 | 85 | 74 | 67 | 63 | 25 | 15 |
| Vesicle area (sample) | 100 | 60 | 55 | 42 | 31 | 21 | 5 |
Using the carboxymethyl chitosan of the carboxymethyl chitosan of low-molecular-weight in embodiment 2 formation 0.2wt% soluble in water
Aqueous solution, taking 2ml carries out human experimentation, and behind 0,3,30,60.90,120,150 minutes after application, determination test position itches
Sense, red and swollen degree and vesicle area are shown in Table 2：
Table 2
Using the carboxymethyl chitosan of the carboxymethyl chitosan of low-molecular-weight in embodiment 3 formation 0.2wt% soluble in water
Aqueous solution, taking 2ml carries out human experimentation, and behind 0,3,30,60.90,120,150 minutes after application, determination test position itches
Sense, red and swollen degree and vesicle area are shown in Table 3：
Table 3
| Time (min) | 0 | 3 | 30 | 60 | 90 | 120 | 150 |
| Red and swollen (positive) | 3.8 | 3.7 | 3.5 | 2.7 | 2.4 | 1.8 | 1.1 |
| Red and swollen (sample) | 3.5 | 2.9 | 2.5 | 1.5 | 0.7 | 0.4 | 0.2 |
| Gargalesthesia (positive) | 3.9 | 3.8 | 3.6 | 2.7 | 1.3 | 1 | 0.4 |
| Gargalesthesia (sample) | 3.8 | 2.2 | 1.3 | 0.8 | 0.2 | 0 | 0 |
| Vesicle area (positive control) | 100 | 89 | 70 | 61 | 46 | 28 | 17 |
| Vesicle area (sample) | 100 | 60 | 52 | 40 | 32 | 15 | 4 |
Using the carboxymethyl chitosan of the carboxymethyl chitosan of low-molecular-weight in embodiment 4 formation 0.2wt% soluble in water
Aqueous solution, taking 2ml carries out human experimentation, and behind 0,3,30,60.90,120,150 minutes after application, determination test position itches
Sense, red and swollen degree and vesicle area are shown in Table 4：
Table 4
, the low-molecular-weight carboxymethyl chitosan tool can be shown according to the result of above-mentioned zoopery and human experimentation
There is good antiallergic effect.
Will be the carboxymethyl chitosan prepared in embodiment 1～4 soluble in water, it is antibacterial to form carboxymethyl chitosan sugar aqueous solution
Liquid.The concentration of the carboxymethyl chitosan sugar aqueous solution is not less than 0.1wt%.
Above-mentioned animal experiment is that human trial data and result show, in the present invention, carboxymethyl chitosan not only has antibacterial
Function, but also with antiallergic and antiinflammatory and antipruritic effect；And due in the present invention by small molecule carboxymethyl chitosan
The antibacterial liquid that formed of aqueous solution there is natural component, which does not simultaneously contain hormoness or other stimulus generalizations are studied point, with good
Good skin-friendly and biocompatibility.
It can be mixed and made into various antibacterial liquids and various dosage forms directly or with other additives, be nursed for Personal hygiene
On articles for use non-woven fabrics, the Personal hygiene nursing materialss non-woven fabrics include diaper for baby and old man, women
The non-woven fabrics used in sanitary towel and wet nonwoven napkin, in the technique for preparing the Personal hygiene nursing materialss,
The surface layer of non-woven fabrics is infiltrated or the antibacterial liquid is sprayed.
A kind of non-woven fabrics are consequently formed, the Personal hygiene nursing materialss include nonwoven layer, and the nonwoven layer is adopted
Antibacterial liquid described above forms antibacterial layer.
Embodiment 5
Using the carboxymethyl chitosan sugar aqueous solution of the formation 1wt% soluble in water of the carboxymethyl chitosan in embodiment 1.
Above-mentioned carboxymethyl chitosan sugar aqueous solution 0.43g, left at room temperature over night are sprayed on the diaper pieces of cloth of 10cm × 10cm
Dry, add urine 10g, 36 DEG C of constant temperature culture.After 100 times of extension rate, it is 100CFU/g to test the clump count for obtaining.
In blank control test, 0.85g pure water on the diaper pieces of cloth of 10cm × 10cm, is sprayed, left at room temperature over night dries in the air
It is dry, add urine 10g, 36 DEG C of constant temperature culture.After 100 times of extension rate, clump count is 1700CFU/g.
Antibacterial difference multiple is 17 times.
Embodiment 6
Using the carboxymethyl chitosan sugar aqueous solution of the formation 1wt% soluble in water of the carboxymethyl chitosan in embodiment 2.
Above-mentioned carboxymethyl chitosan sugar aqueous solution 0.43g, left at room temperature over night are sprayed on the diaper pieces of cloth of 10cm × 10cm
Dry, add urine 10g, 36 DEG C of constant temperature culture.After 100 times of extension rate, it is 10200CFU/g to test the clump count for obtaining.
In blank control test, 0.85g pure water on the diaper pieces of cloth of 10cm × 10cm, is sprayed, left at room temperature over night dries in the air
It is dry, add urine 10g, 36 DEG C of constant temperature culture.After 100 times of extension rate, clump count is 640000CFU/g.
Antibacterial difference multiple is 62 times.
Embodiment 7
Using the carboxymethyl chitosan sugar aqueous solution of the formation 1wt% soluble in water of the carboxymethyl chitosan in embodiment 3.
Above-mentioned carboxymethyl chitosan sugar aqueous solution 0.43g, left at room temperature over night are sprayed on the diaper pieces of cloth of 10cm × 10cm
Dry, add urine 10g, 36 DEG C of constant temperature culture.After 100 times of extension rate, it is 3040000CFU/g to test the clump count for obtaining.
In blank control test, 0.85g pure water on the diaper pieces of cloth of 10cm × 10cm, is sprayed, left at room temperature over night dries in the air
It is dry, add urine 10g, 36 DEG C of constant temperature culture.After 100 times of extension rate, clump count is 14000000CFU/g.
Antibacterial difference multiple is 4 times.
Which is not only convenient when using and prepare for the aqueous solution of above-mentioned carboxymethyl chitosan, and have antibacterial antiallergic,
Antiinflammatory and antipruritic effect are good, colorless and odorless, and which is natural small molecule material, and biocompatibility and skin-friendly are good, for baby
Can be very effective when on the non-woven fabrics used on the diaper of youngster and old man, sanitary towel for women and wet nonwoven napkin
The user for avoiding above-mentioned articles for use occur in use redness, the skin problem of prurituss etc..
Antibacterial liquid described above is used in the technique for preparing non-woven fabrics, the surface layer of non-woven fabrics is infiltrated or the suppression is sprayed
Bacterium solution so as to reach good fungistatic effect, meets the use demand of user so that the surface layer of non-woven fabrics forms antibacterial layer.
The above, only presently preferred embodiments of the present invention, not any formal and substantial to present invention restriction,
It should be pointed out that for those skilled in the art, on the premise of without departing from the inventive method, can also make
Some improvement and supplement, these improve and supplement also should be regarded as protection scope of the present invention.All those skilled in the art,
Without departing from the spirit and scope of the present invention, when make using disclosed above technology contents it is a little more
Dynamic, modification and the equivalent variations for developing, are the Equivalent embodiments of the present invention；Meanwhile, all substantial technologicals pair according to the present invention
Change, modification and the differentiation of any equivalent variations that above-described embodiment is made, still falls within the scope of technical scheme
It is interior.
Claims (10)
1. a kind of antibacterial liquid, it is characterised in that：Described antibacterial liquid is the aqueous solution containing carboxymethyl chitosan, the carboxylic first
The number-average molecular weight of base enclosure polysaccharide is 1000～20000.
2. antibacterial liquid according to claim 1, it is characterised in that：The antibacterial liquid also include following characteristics in one kind or
It is various：
The concentration of the carboxymethyl chitosan sugar aqueous solution is not less than 0.1wt%；
The carboxymethyl chitosan is O- carboxymethylchitosans.
3. antibacterial liquid according to claim 1, it is characterised in that：The preparation method of the carboxymethyl chitosan is included number
The higher carboxymethyl chitosan raw material of average molecular weight is degraded by hydrogen peroxide, the higher carboxymethyl chitosan of the number-average molecular weight
The number-average molecular weight 30～700,000 of carboxymethyl chitosan in raw material.
4. antibacterial liquid according to claim 2, it is characterised in that：The higher carboxymethyl chitosan glycogen of the number-average molecular weight
In material, degree of substitution by carboxymethyl is 0.8～1.3.
5. antibacterial liquid according to claim 2, it is characterised in that：Hydrogen peroxide degraded technique be：By hydrogen peroxide in stirring
Under the conditions of add the higher carboxymethyl chitosan of number-average molecular weight in carry out degradation reaction, during degradation reaction plus alkali is caused instead
The pH for answering system is 8.0～8.5, until sodium sulfite stopped reaction is added when the viscosity of reaction system is 80～120cp.
6. antibacterial liquid according to claim 2, it is characterised in that：The preparation method of the carboxymethyl chitosan is included to double
Carboxymethyl chitosan after the degraded of oxygen water carries out acid out washing.
7. antibacterial liquid according to claim 5, it is characterised in that：The acid out washing is referred to acid adjustment hydrogen peroxide degraded
Carboxymethyl chitosan sugar material body afterwards is 6～7 to pH, adds ethanol to separate out to light yellow crystal, then uses ethanol cyclic washing, gets rid of
It is dry, it is dried and obtains carboxymethyl chitosan.
8. purposes of a kind of antibacterial liquid as described in any one of claim 1～7 on sanitary care article is prepared.
9. purposes as claimed in claim 8, it is characterised in that：The Personal hygiene nursing materialss include nonwoven layer, described
Nonwoven layer forms antibacterial layer using the antibacterial liquid described in claim 1～8.
10. purposes as claimed in claim 9, it is characterised in that：Nonwoven layer on Personal hygiene nursing materialss include for
The nonwoven layer used on the diaper of baby and old man, sanitary towel for women, wet nonwoven napkin and facial film.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610946256.0A CN106511370A (en) | 2016-11-02 | 2016-11-02 | Bacteriostatic liquid |
| PCT/CN2016/111195 WO2018082163A1 (en) | 2016-11-02 | 2016-12-21 | Bacteriostatic liquid |
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| CN201610946256.0A CN106511370A (en) | 2016-11-02 | 2016-11-02 | Bacteriostatic liquid |
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