CN106483275A - A kind of low-temperature biological experimental provision - Google Patents
A kind of low-temperature biological experimental provision Download PDFInfo
- Publication number
- CN106483275A CN106483275A CN201611066265.7A CN201611066265A CN106483275A CN 106483275 A CN106483275 A CN 106483275A CN 201611066265 A CN201611066265 A CN 201611066265A CN 106483275 A CN106483275 A CN 106483275A
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- Prior art keywords
- dewar bottle
- experimental provision
- temperature
- low
- liquid nitrogen
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 26
- 238000005259 measurement Methods 0.000 claims abstract description 7
- 238000000151 deposition Methods 0.000 claims abstract description 4
- 239000012530 fluid Substances 0.000 claims description 7
- 238000001802 infusion Methods 0.000 claims description 7
- 239000002131 composite material Substances 0.000 claims description 4
- 229910001006 Constantan Inorganic materials 0.000 claims description 3
- 210000005239 tubule Anatomy 0.000 claims description 3
- 229910000831 Steel Inorganic materials 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 239000010959 steel Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 10
- 238000012360 testing method Methods 0.000 abstract description 4
- 239000011152 fibreglass Substances 0.000 description 6
- 238000001816 cooling Methods 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000012620 biological material Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000002826 coolant Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
Abstract
The invention discloses a kind of low-temperature biological experimental provision, it is characterised in that：Including the Dewar bottle for depositing heat-conduction medium and cooled sample, temperature measuring and recording module is provided with the Dewar bottle, liquid nitrogen liquid level automatic adjuster is provided with outside the Dewar bottle, liquid level for keeping the outer liquid nitrogen of Dewar bottle is constant, the Dewar bottle is connected with the mechanical pump for vacuumizing, and the mechanical pump is connected to vacuum measurement module and vacuum governor.This apparatus structure is simple, and control method is novel, convenient, and stability preferably, can meet general biologic test and require.This apparatus structure is simple, is vacuumized using Dewar bottle cooperative mechanical pump using hot method temperature control is leaked, and control method is novel, convenient, and stability is preferable, and low cost can meet general biologic test and require.
Description
【Technical field】
The invention belongs to Bioexperiment equipment technical field, and in particular to a kind of low-temperature biological experimental provision.
【Background technology】
Recently, the application of cryobiology and cryogenic medicine is more and more extensive, biological tissue and cell for example blood, marrow,
The Cord blood of embryo, skin etc. has highly important practical significance.However, after having many factors impact chilled to thaw
The survival rate of biomaterial, one of most important of which factor are exactly cooldown rate.Different cells require different cooldown rates,
A series of test according to different cooling rate, it may be determined that the Investigation of Optimum Cooling Condition of certain cell.
【Content of the invention】
The technical problem to be solved is, for above-mentioned deficiency of the prior art, to provide a kind of low-temperature biological
Experimental provision, can determine control parameter previously according to the requirement of rate of temperature fall, and good stability is simple, effective and easy to use
Temperature regulating device.
The present invention is employed the following technical solutions：
A kind of low-temperature biological experimental provision, including the Dewar bottle for depositing heat-conduction medium and cooled sample, described
Temperature measuring and recording module is provided with Dewar bottle, is provided with liquid nitrogen liquid level automatic adjuster, for protecting outside the Dewar bottle
The liquid level for holding the outer liquid nitrogen of Dewar bottle is constant, and the Dewar bottle is connected with the mechanical pump for vacuumizing, and the mechanical pump divides
Vacuum measurement module and vacuum governor are not connected with.
Further, multilayer insulant is provided between the inside and outside wall of the Dewar bottle, connected by the tubule of upper end
The mechanical pump is used for vacuumizing.
Further, the inner and outer wall of the Dewar bottle is made up of fiberglass.
Further, the liquid nitrogen liquid level automatic adjuster includes controller and the reservoir being separated into by cut-off and placement
Groove, is provided with liquid nitrogen in the standing groove, and the Dewar bottle is arranged in the standing groove, between the reservoir and standing groove
Connected by woven hose, the controller is connected with the reservoir and standing groove respectively, for liquid nitrogen in the standing groove
Supplemented by the reservoir when lacking.
Further, adding thermal resistance is provided with the reservoir, the adding thermal resistance is connected with the controller, is used for
The pressure improved in the reservoir carries out fluid infusion.
Further, pressure sensor is provided with the standing groove, the pressure sensor is connected with the controller,
For detecting the height of the liquid nitrogen in the standing groove.
Further, the vacuum governor is fine setting needle valve, for controlling discharge quantity.
Further, the vacuum measurement module includes thermocouple rule and the composite evacuated instrument of digital display.
Further, the temperature measuring and recording module includes copper-constantan thermocouple, and precision is ± 0.5K.
Compared with prior art, the present invention at least has the advantages that：
This apparatus structure is simple, is vacuumized using Dewar bottle cooperative mechanical pump using hot method temperature control is leaked, control method
Novel, convenient, stability is preferable, and low cost can meet general biologic test and require.
Further, as experimental provision, simple structure, heat-insulating property are good, and transparent, tightness is good, adopt for Dewar bottle
Mechanical pump carries out vacuumizing process, and with low cost, economy and durability carries out vacuum conditions, precise control vacuum by fine setting needle valve
Degree.
Further, thermocouple advises the heat transfer phenomenon using gas molecule, and the pressure limit that may be measured is in 1～300Pa
Between, circuit is simple, and cheap, the composite evacuated instrument of digital display can show vacuum in real time.
Below by drawings and Examples, technical scheme is described in further detail.
【Description of the drawings】
Fig. 1 is present system structure chart；
Fig. 2 is Dewar bottle structure chart of the present invention；
Fig. 3 is liquid nitrogen liquid level automatic adjuster schematic diagram of the present invention.
Wherein, 1. controller；2. reservoir；3. woven hose；4. pressure sensor；5. adding thermal resistance；6. standing groove.
【Specific embodiment】
Refer to shown in Fig. 1, the invention provides a kind of low-temperature biological experimental provision, it is characterised in that：Including for depositing
Heat release transmitting medium and the Dewar bottle of cooled sample, are provided with temperature measuring and recording module, the Dewar in the Dewar bottle
Bottle is outside to be provided with liquid nitrogen liquid level automatic adjuster, and the liquid level for keeping the outer liquid nitrogen of Dewar bottle is constant, the Dewar bottle
The mechanical pump being connected with for vacuumizing, the mechanical pump are connected to vacuum measurement module and vacuum governor.
Liquid nitrogen liquid level automatic adjuster can keep the liquid level of the outer liquid nitrogen of fiberglass Dewar bottle constant, make operating process steady
Fixed.
The liquid nitrogen liquid level automatic adjuster includes controller 1 and the reservoir 2 being separated into by cut-off and standing groove 6, institute
State in standing groove 6 and liquid nitrogen is provided with, the Dewar bottle is arranged in the standing groove 6, between the reservoir 2 and standing groove 6
Connected by woven hose 3, the controller 1 is connected with the reservoir 2 and standing groove 6 respectively, in the standing groove 6
Supplemented by the reservoir 2 when liquid nitrogen lacks.Adding thermal resistance 5, the adding thermal resistance 5 are provided with the reservoir 2
It is connected with the controller 1, the pressure for improving in the reservoir 2 carries out fluid infusion.Pressure is provided with the standing groove 6
Sensor 4, the pressure sensor 4 are connected with the controller 1, for detecting the height of the liquid nitrogen in the standing groove 6.
Whole fluid infusion process is specially：When liquid level is less than 10cm, controller starts fluid infusion process, closes solenoid, closes
Upper adding thermal resistance, heats the liquid nitrogen in reservoir so that pressure rise in reservoir, starts fluid infusion, when supplementary liquid is higher than
During 35cm, stop fluid infusion, adding thermal resistance is turned off, open magnetic valve deflation.
Vacuum governor using fine setting needle valve, for controlling discharge quantity, the pumping amount phase equilibrium that is allowed to mechanical pump, make
Multilayer heat insulation space keeps the vacuum of a certain requirement.
Vacuum measurement module is opened as needle valve is adjusted using thermocouple rule and the monitoring system of the composite evacuated instrument composition of digital display
The foundation of degree.
Temperature survey adopts copper-constantan thermocouple, is ± 0.5K through calibrated precision, and sensitivity is 40 × 10-6V/K.
Refer to shown in Fig. 2, Dewar bottle inner bag deposits heat-conduction medium and cooled sample, and Dewar bottle outer wall is by liquid nitrogen
Cooling, and multilayer insulant is housed between inside and outside wall, evacuated by the tubule of upper end, adjusting different vacuums is
Different leaking heats are obtained, to control different cooldown rates.
Assume that fiberglass inwall, outer wall and multilayer material do not have vertical temperature difference, i.e., only consider the one dimensional heat transfer of radial direction.
While assuming the temperature of cooling medium and sample uniformity all the time, thus can process by lump Heat-Capacity System.Cooling is situated between
The heat that matter and sample are scattered to liquid nitrogen by the inside and outside wall of fiberglass and the leakage of vacuum multi-layer insulation material isWherein,
R=ROutward+RInterior+Rk, ROutwardFor fiberglass outer wall thermal resistance, RInteriorFor fiberglass inwall thermal resistance, RkThermal resistance for vacuum multi-layer insulation layer.
Above content technological thought only to illustrate the invention, it is impossible to limit protection scope of the present invention with this, every presses
According to technological thought proposed by the present invention, any change that is done on the basis of technical scheme, claims of the present invention is each fallen within
Protection domain within.
Claims (9)
1. a kind of low-temperature biological experimental provision, it is characterised in that：Including Du for depositing heat-conduction medium and cooled sample
Watt bottle, is provided with temperature measuring and recording module, is provided with liquid nitrogen liquid level and automatically adjusts outside the Dewar bottle in the Dewar bottle
Device, the liquid level for keeping the outer liquid nitrogen of Dewar bottle are constant, and the Dewar bottle is connected with the mechanical pump for vacuumizing, described
Mechanical pump is connected to vacuum measurement module and vacuum governor.
2. a kind of low-temperature biological experimental provision according to claim 1, it is characterised in that：The inside and outside wall of the Dewar bottle
Between be provided with multilayer insulant, connecting the mechanical pump by the tubule of upper end is used for vacuumizing.
3. a kind of low-temperature biological experimental provision according to claim 2, it is characterised in that：The inwall of the Dewar bottle is with outward
Wall is glass steel.
4. a kind of controlled low-temperature biological experimental provision of rate of temperature fall according to claim 1, it is characterised in that the liquid
Nitrogen liquid level automatic adjuster includes controller (1) and the reservoir (2) being separated into by cut-off and standing groove (6), the standing groove
(6) liquid nitrogen is provided with, the Dewar bottle is arranged in the standing groove (6), between reservoir (2) and standing groove (6)
Connected by woven hose (3), controller (1) is connected with the reservoir (2) and standing groove (6) respectively, for described
Supplemented by reservoir (2) when the interior liquid nitrogen of standing groove (6) lacks.
5. a kind of controlled low-temperature biological experimental provision of rate of temperature fall according to claim 4, it is characterised in that the storage
Adding thermal resistance (5) is provided with liquid bath (2), adding thermal resistance (5) are connected with the controller (1), for improving the storage
Pressure in liquid bath (2) carries out fluid infusion.
6. the controlled low-temperature biological experimental provision of a kind of rate of temperature fall according to claim 4, it is characterised in that described put
Put in groove (6) and pressure sensor (4) is provided with, pressure sensor (4) are connected with the controller (1), for detecting
State the liquid nitrogen height in standing groove (6).
7. a kind of low-temperature biological experimental provision according to claim 1, it is characterised in that the vacuum governor is fine setting
Needle valve, for controlling discharge quantity.
8. a kind of low-temperature biological experimental provision according to claim 1, it is characterised in that：The vacuum measurement module includes
Thermocouple rule and the composite evacuated instrument of digital display.
9. a kind of low-temperature biological experimental provision according to claim 1, it is characterised in that：The temperature measuring and recording module
Including copper-constantan thermocouple, precision is ± 0.5K.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611066265.7A CN106483275A (en) | 2016-11-28 | 2016-11-28 | A kind of low-temperature biological experimental provision |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611066265.7A CN106483275A (en) | 2016-11-28 | 2016-11-28 | A kind of low-temperature biological experimental provision |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106483275A true CN106483275A (en) | 2017-03-08 |
Family
ID=58275582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611066265.7A Pending CN106483275A (en) | 2016-11-28 | 2016-11-28 | A kind of low-temperature biological experimental provision |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106483275A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109520908A (en) * | 2018-12-29 | 2019-03-26 | 中国科学技术大学 | A kind of low temperature test device based on physical adsorption appearance and automatic liquid feeding nitrogen device |
| CN114383926A (en) * | 2021-12-23 | 2022-04-22 | 苏州圣苏新药开发有限公司 | Biological analysis low temperature experimental apparatus |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2129863A7 (en) * | 1971-03-17 | 1972-11-03 | Comp Generale Electricite | Cryogenic vessel - for experimental physics |
| CN2682849Y (en) * | 2004-02-24 | 2005-03-09 | 武汉化工学院 | Small-sized low-temperature creature freezing instrument |
| CN1916795A (en) * | 2006-09-11 | 2007-02-21 | 中国科学院上海技术物理研究所 | Automatic control system and method for cyclic test in multiple service positions and at high and low temperatures |
| JP2008048800A (en) * | 2006-08-23 | 2008-03-06 | Hitachi High-Technologies Corp | Dewar and biomagnetism measuring device with the same |
| KR101110798B1 (en) * | 2010-10-27 | 2012-02-24 | 한국표준과학연구원 | Dewar for biological magnetism measurement apparatus, biological magnetism measurement apparatus including the same and manufacturing method thereof |
-
2016
- 2016-11-28 CN CN201611066265.7A patent/CN106483275A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2129863A7 (en) * | 1971-03-17 | 1972-11-03 | Comp Generale Electricite | Cryogenic vessel - for experimental physics |
| CN2682849Y (en) * | 2004-02-24 | 2005-03-09 | 武汉化工学院 | Small-sized low-temperature creature freezing instrument |
| JP2008048800A (en) * | 2006-08-23 | 2008-03-06 | Hitachi High-Technologies Corp | Dewar and biomagnetism measuring device with the same |
| CN1916795A (en) * | 2006-09-11 | 2007-02-21 | 中国科学院上海技术物理研究所 | Automatic control system and method for cyclic test in multiple service positions and at high and low temperatures |
| KR101110798B1 (en) * | 2010-10-27 | 2012-02-24 | 한국표준과학연구원 | Dewar for biological magnetism measurement apparatus, biological magnetism measurement apparatus including the same and manufacturing method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 黄志秀等: "降温速率可控的低温生物实验装置", 《低温与超导》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109520908A (en) * | 2018-12-29 | 2019-03-26 | 中国科学技术大学 | A kind of low temperature test device based on physical adsorption appearance and automatic liquid feeding nitrogen device |
| CN114383926A (en) * | 2021-12-23 | 2022-04-22 | 苏州圣苏新药开发有限公司 | Biological analysis low temperature experimental apparatus |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170308 |
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| RJ01 | Rejection of invention patent application after publication |