CN2682849Y - Small-sized low-temperature creature freezing instrument - Google Patents
Small-sized low-temperature creature freezing instrument Download PDFInfo
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- CN2682849Y CN2682849Y CN 200420017227 CN200420017227U CN2682849Y CN 2682849 Y CN2682849 Y CN 2682849Y CN 200420017227 CN200420017227 CN 200420017227 CN 200420017227 U CN200420017227 U CN 200420017227U CN 2682849 Y CN2682849 Y CN 2682849Y
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- small
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- sealing shroud
- frigorimeter
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Abstract
A small-sized low-temperature creature freezing instrument used for vitrifying fast cooling and preserving biological samples of the utility model comprises a mini vacuum pump, a sealing sleeve provided with an aspirating hole and a lid, a small-sized liquid nitrogen Dewar, a heat exchanger, a diffuser, a temperature sensor, a vacuum gauge, a number display, an automatic controller, etc. With the aspirating voltage-drop method, the liquid nitrogen can be boiled to transfer heat and subcooled and the air is repressed. The automatic controller controls the vacuum pump and a repressing switch and the overall process is automatically displayed; the operating temperature can be adjusted between 196 degrees below zero and 209 degrees below zero.
Description
Technical field the utility model relates to the cryogenic engineering technical field, is the small-sized low-temperature biological frigorimeter of the supper-fast cooling preservation biological sample of a kind of vitrifying.
Background technology biomaterial low temperature preserve the formation that one of the damage caused is an ice in the biological cell (Hua Zezhao, Ren Hesheng. the low-temperature biological medical technology.Beijing: Science Press, 1994), ice crystal can destroy the orderly arrangement of interior atom of cell or bion, the reason of freezing death that Here it is.Vitrifying is a kind of new method of biological cell preservation in recent years.So-called vitrifying is meant that liquid changes the solidification process of amorphous state (glassy state) into.It is different with the freezing process of solid that common liquid changes crystal or partially crystallizable into, relation between the glassy solids molecule and liquid no significant change, thereby cause that the structural change of biological cell is little.It is cell and protectant thereof to be crossed with very fast rate of temperature fall be as cold as glass transition temperature that the vitrifying low temperature of biomaterial is preserved, and with this glassy state long preservation at low temperatures, to avoid owing to freeze to the damage of biological cell.
Realize the vitrifying of the aqueous solution, require to have very high cooldown rate.For pure water, require 106K/s; For general solution, require 10
3To 10
4K/s; For concentrated solution, promptly so-called vitrification solution requires 10
2To 10
3K/s (Li Guangwu, Zheng Congyi, Tang Bing.Cryobiology.Hunan Science Press, 1998).Biological sample is directly immersed liquid nitrogen, even be about 0.2 small sample for diameter, cooldown rate also can only reach about 100K/s.This be because be in the sample of room temperature can the vapor film above liquid nitrogen in by " precooling ", and the gas phase heat-transfer effect is poor; And after sample enters liquid nitrogen, can make sample thin layer liquid nitrogen vaporization on every side, produce air film, also suppress the raising of cooldown rate.
By the vapour pressure of control reduction liquid nitrogen, obtain being lower than the liquid nitrogen (overfreezing liquid nitrogen) of 77K, recover normal pressure again.Make atom sample rapid movement in overfreezing liquid nitrogen, avoid producing boiling at contact-making surface when (77K) sample contacts with liquid nitrogen when boiling point and form air film, the sample heat is directly imported in the overfreezing liquid nitrogen, can improve the rate of temperature fall of sample.Adopt overfreezing liquid nitrogen to be applied to the freezing of biological sample such as sperm, ovum etc.; because rate of temperature fall increases, and helps the vitrifying of cryoprotector, can reduce the protectant concentration of frozen liq; alleviate refrigeration toxicity and damage, improve the quality of refrigeration biological sample.
In the low-temperature biological engineering, the normal at present program frigorimeter that adopts import is realized the low temperature preservation of lowering the temperature at a slow speed.The program frigorimeter costs an arm and a leg, and during operating cost, effect is not good enough, thereby the quick cooled glass low temperature of biomaterial is preserved the attention that technology and low temperature technique for fixing are subjected to low-temperature biological circle always.
Preserve technology about vitrifying low temperature, be in laboratory stage at present both at home and abroad basically, the device therefor volume is big, and complicated operation is difficult to commercialization.The utility model is a kind of small-sized cryogenic freezing device.Its volume is little, and is in light weight, and performance is good, and its minimum temperature can reach-210 ℃ of degree.Can be used widely in fields such as biology, medical science, livestock breeding, food industry and new function material manufacturings.
Summary of the invention the utility model is the midget plant of the supper-fast cooling preservation biological sample of a kind of vitrifying, mainly form, it is characterized in that above-mentioned device is combined in the small box according to the order of vavuum pump, heat exchanger, the sealing shroud that has aspirating hole, Dewar container for liquefied nitrogen, pressure and temperature sensor, various Displaying Meters by vavuum pump, the sealing shroud that has aspirating hole, Dewar container for liquefied nitrogen, heat exchanger, pressure and temperature sensor and various Displaying Meter.It is preserved for the vitrifying low temperature of biomaterial a kind of small-sized instrument easily is provided.The Dewar of splendid attire liquid nitrogen adopts stainless-steel vacuum thermos cup or glass evacuated cup, is positioned in the cylindrical enclosure sealing shroud by low temperature resistant polytetrafluoroethylene (PTFE), stainless steel or copper one-tenth.Sealing shroud is drilled with special aperture, is used to bleed and answers pressure; The top mouth of sealing shroud and lid are handled through the minute surface grinding machine, become plane contact, no packing ring, and no screw thread, air tight and easy to operate.The multiple pressure valve that is provided with between sealing shroud and the atmospheric communicating pipe is an automatic valve.Temperature sensor places in the Dewar container for liquefied nitrogen, and pressure sensor places in the sealing shroud, connects with display through automatic control system.
The utility model has the advantages that: (1) volume is little, in light weight, needs liquid nitrogen few, is easy to carry; (2) the reliability height is easy to operate; (3) operating temperature can be reached in the short period of time and operating temperature can be regulated automatically.
Appended drawings is the interior schematic diagram of this device.1 is display among the figure, and 2 is controller, the 3rd, and automatic valve, the 4th, minipump, the 5th, diffuser, the 6th, atmospheric communicating pipe, 7 is sealing shroud, the running-on mirror finish, and wherein one side is finished to minute surface, the 8th, small-sized Dewar bottle, the 9th, radiator, the 10th, vacuum meter, the 11st, aspirating hole, the 12nd, sealing upper shield, the 13rd, temperature sensor, the 14th, amplifier, the 15th, analog-digital converter, the 16th, power supply.
Embodiment the utility model is the midget plant of the supper-fast cooling preservation biological sample of a kind of vitrifying, mainly form, it is characterized in that above-mentioned device is combined in the small box according to the order of vavuum pump, heat exchanger, the sealing shroud that has aspirating hole, Dewar container for liquefied nitrogen, pressure and temperature sensor, various Displaying Meters by vavuum pump, the sealing shroud that has aspirating hole, Dewar container for liquefied nitrogen, heat exchanger, pressure and temperature sensor and various Displaying Meter.Normal temperature liquid nitrogen (subzero 196 ℃) is poured in the Dewar container for liquefied nitrogen, covered the sealing upper shield, open minipump, the sealing shroud internal pressure descends, and liquid-nitrogen boiling conducts heat and be cold excessively.The temperature of liquid nitrogen or degree of supercooling depend on the pressure in the sealing shroud, by regulating this pressure, can make overfreezing liquid nitrogen temperature (subzero 210 ℃ time, nitrogen has been solid-state) between subzero 196 ℃ to subzero 209 ℃ adjustable.When liquid nitrogen cooling during to default temperature, vavuum pump stops automatically, and automatic valve is opened, and air enters sealing shroud through diffuser and presses again.When the pressure of sealing shroud is depressed into atmospheric pressure again, open the sealing upper shield, biological sample is launched in the overfreezing liquid nitrogen.
Claims (6)
1. small-sized low-temperature biological frigorimeter, mainly form, it is characterized in that above-mentioned device is combined in the small box according to the order of vavuum pump, heat exchanger, the sealing shroud that has aspirating hole, Dewar container for liquefied nitrogen, pressure and temperature sensor, various Displaying Meters by vavuum pump, the sealing shroud that has aspirating hole, Dewar container for liquefied nitrogen, heat exchanger, pressure and temperature sensor and various Displaying Meter.
2. a kind of small-sized low-temperature biological frigorimeter according to claim 1 is characterized in that adopting minipump (4).
3. a kind of small-sized low-temperature biological frigorimeter according to claim 1 is characterized in that the interface of sealing shroud (7) and seal cover (12) is a plane contact.
4. according to claim 1 or 3 described a kind of small-sized low-temperature biological frigorimeters, it is characterized in that sealing shroud (7) is become by low temperature resistant polytetrafluoroethylene (PTFE), stainless steel or copper with seal cover (12).
5. according to claim 1 or 3 described a kind of small-sized low-temperature biological frigorimeters, it is characterized in that Dewar container for liquefied nitrogen adopts stainless-steel vacuum thermos cup or glass evacuated cup.
6. a kind of small-sized low-temperature biological frigorimeter according to claim 1 is characterized in that the multiple pressure valve that is provided with is automatic valve (3) between sealing shroud and atmospheric communicating pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420017227 CN2682849Y (en) | 2004-02-24 | 2004-02-24 | Small-sized low-temperature creature freezing instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420017227 CN2682849Y (en) | 2004-02-24 | 2004-02-24 | Small-sized low-temperature creature freezing instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2682849Y true CN2682849Y (en) | 2005-03-09 |
Family
ID=34606506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200420017227 Expired - Fee Related CN2682849Y (en) | 2004-02-24 | 2004-02-24 | Small-sized low-temperature creature freezing instrument |
Country Status (1)
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| CN (1) | CN2682849Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100462004C (en) * | 2007-09-28 | 2009-02-18 | 浙江大学 | Low temperature protectant solution program controlled refrigerating mechanism for vitrified preservation |
| CN102125026A (en) * | 2011-01-26 | 2011-07-20 | 沈阳航天新光低温容器制造有限责任公司 | Program-controlled freezing instrument |
| CN104106564A (en) * | 2014-04-17 | 2014-10-22 | 衢州兰玲机电科技有限公司 | Sperm refrigeration box |
| CN106483275A (en) * | 2016-11-28 | 2017-03-08 | 榆林学院 | A kind of low-temperature biological experimental provision |
| CN109997841A (en) * | 2019-04-09 | 2019-07-12 | 上海交通大学 | Maintain mankind's gamete fast freeze-thaw visible environment cabin of atmospheric air atmosphere |
| WO2023143115A1 (en) * | 2022-01-28 | 2023-08-03 | 上海明悦医疗科技有限公司 | Freezing system |
-
2004
- 2004-02-24 CN CN 200420017227 patent/CN2682849Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100462004C (en) * | 2007-09-28 | 2009-02-18 | 浙江大学 | Low temperature protectant solution program controlled refrigerating mechanism for vitrified preservation |
| CN102125026A (en) * | 2011-01-26 | 2011-07-20 | 沈阳航天新光低温容器制造有限责任公司 | Program-controlled freezing instrument |
| CN104106564A (en) * | 2014-04-17 | 2014-10-22 | 衢州兰玲机电科技有限公司 | Sperm refrigeration box |
| CN106483275A (en) * | 2016-11-28 | 2017-03-08 | 榆林学院 | A kind of low-temperature biological experimental provision |
| CN109997841A (en) * | 2019-04-09 | 2019-07-12 | 上海交通大学 | Maintain mankind's gamete fast freeze-thaw visible environment cabin of atmospheric air atmosphere |
| CN109997841B (en) * | 2019-04-09 | 2021-02-02 | 上海交通大学 | Human gamete rapid freezing and thawing visualization environment cabin maintaining atmospheric air atmosphere |
| WO2023143115A1 (en) * | 2022-01-28 | 2023-08-03 | 上海明悦医疗科技有限公司 | Freezing system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20050309 Termination date: 20100224 |