CN2208205Y - Anti-ice-crystal deep-freezing device for liquid sample - Google Patents
Anti-ice-crystal deep-freezing device for liquid sample Download PDFInfo
- Publication number
- CN2208205Y CN2208205Y CN 94248681 CN94248681U CN2208205Y CN 2208205 Y CN2208205 Y CN 2208205Y CN 94248681 CN94248681 CN 94248681 CN 94248681 U CN94248681 U CN 94248681U CN 2208205 Y CN2208205 Y CN 2208205Y
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- freezing
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Abstract
The utility model discloses an anti-ice-crystal deep-freezing device for liquid samples, which is used for preparation of liquid samples of an electronic microscope. The utility model can control the injection amount of the samples through a fixed lifting frame. The utility model adopts the freezing block of a conical groove made of copper or silver materials of high conducting as a freezing carrier to treat samples. Then the purpose of deep freezing can be achieved and the imagination of preventing ice crystal is realized. The samples which are obtained by the utility model can make the viewing visual field of an electronic microscope be enlarged and the microviewing state of the samples is clear.
Description
The utility model relates to electron microscope liquid sample preparation field.Especially be used for preparing the anti-ice crystal cryogenic quick-freezing device of liquid sample.
Look into according to document, have the multiple report that electron microscope prepares sampling device that is used for both at home and abroad.For example: the external mechanical insertion device that uses, complex structure is done the indirect refrigeration agent with liquid nitrogen, and propane is done direct refrigerant, and propane is inflammable dangerous, and the operating procedure complexity.The domestic bronze mirror cryofixation device of also having made, immersion snap frozen device, and freezing pincers snap frozen device etc., suchlike device is used for the preparation of moisture abundant solid samples such as biological sample more.
Task of the present utility model is existing " the freezing method for making sample that is used for the electronic microscope observation of liquid state sample " (patent publication No. CN1061923A) used sample preparation apparatus is improved, to obtain the sample preparation apparatus of better realization the method.Liquid sample more is rich in water than biological sample, and is bad because of freezing rate control in refrigerating process, produces ice crystal.Because ice crystal disturbs, and has destroyed sample microstructure on the replica film, the electron microscopic observation visual field is reduced, also may influence film forming when serious, the realization of the utility model device is sent in this point just.
Fig. 1 is the utility model front view
Fig. 2 is the utility model block diagram
The utility model Fig. 1,1 base shown in 2,2 supports, 3,4 knobs, 5,7 cross bars, 6 syringes, 8 haemostatic clamp, 9 frozen block, 10 freezing stages, 11 liquid nitrogen bottles, 12 cone tanks (hole), the liquid sample of 13 locating holes is prevented ice crystal cryogenic quick-freezing device, it is characterized in that being fixed on the stand type support (2) on the base (1) liftable knob (3) is housed, (4) and respectively be fixed with syringe (6), the cross bar (5) of freezing stage (10), (7) link to each other, syringe (6), freezing stage (10) is vertical with liquid nitrogen bottle (11) on the base (1), haemostatic clamp (8) inserts the frozen block (9) that is carved with cone tank (12) in locating hole (13) operation and places freezing stage (10) face, freezing stage (10), the copper that is made as high heat conduction or the ag material of frozen block (9).
The optimum implementation of device described in the utility model is: be fixed in the syringe regulation and control sample size that the base support top is communicated with cross bar, frozen block bellmouth (groove) on the freezing table top of alignment bracket below connection cross bar, before sample injects, frozen block is closed by the haemostatic clamp operation, together with freezing stage one landing end give in the liquid nitrogen bottle cold, when freezing stage and frozen block reach liquid nitrogen temperature, can rise liquid sample is splashed into the liquid sample cone tank, sample quenching system liquid nitrogen temperature, again frozen block is opened, the good ice hockey of cryogenic quick-freezing is collected in the groove of freezing stage, then ice hockey is bonded in the sample holder and cuts off, corrosion, electron microscopic observation is treated in vacuum evaporating etc.Knob control cross bar on the support slides up and down with handled easily.Frozen block and freezing stage all use highly heat-conductive material copper or silver to make, and enough big thermal capacity is arranged.When reaching liquid nitrogen temperature, can keep the long period, be beneficial to the post processing of sample, reduce the chance that produces ice crystal, tapered groove is set in the frozen block, up big and down small, be easy to drop and fall into the bottom, can reach the effect that moment freezes anti-ice crystal suddenly, the good ice hockey of quick-frozen also just and the aperture of sample holder be complementary, made things convenient for the sample ice hockey to be stained with the operation of sample holder.
In sum, be used for the anti-ice crystal cryogenic quick-freezing device of Electronic Speculum liquid sample, go up on probation in corrosion method (obtaining silver medal on the international show of inventions in Beijing), effect is excellent, now no longer perplexed by liquid sample produces ice crystal, the electron microscopic observation visual field enlarges, and microscopic pattern is high-visible.
Claims (1)
1, the anti-ice crystal cryogenic quick-freezing device of liquid sample, it is characterized in that being fixed on liftable knob (3), (4) being housed on the stand type support (2) on the base (1) and being fixed with syringe (6), the cross bar (5) of freezing stage (10), (7) respectively and link to each other, syringe (6), freezing stage (10) are vertical with liquefied ammonia bottle (11) on the base (1), haemostatic clamp inserts the frozen block (9) that is carved with cone tank (12) in locating hole (8) operation and places freezing stage (10) face, freezing stage (10), frozen block (9) be made as copper or the ag material that height is led.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94248681 CN2208205Y (en) | 1994-12-21 | 1994-12-21 | Anti-ice-crystal deep-freezing device for liquid sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94248681 CN2208205Y (en) | 1994-12-21 | 1994-12-21 | Anti-ice-crystal deep-freezing device for liquid sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2208205Y true CN2208205Y (en) | 1995-09-20 |
Family
ID=33853535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94248681 Expired - Fee Related CN2208205Y (en) | 1994-12-21 | 1994-12-21 | Anti-ice-crystal deep-freezing device for liquid sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2208205Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103335478A (en) * | 2013-07-25 | 2013-10-02 | 钦龙金属工业（昆山）有限公司 | Lifting frame for lifting type liquid refrigerating equipment |
| CN103340467A (en) * | 2013-07-25 | 2013-10-09 | 钦龙金属工业（昆山）有限公司 | Quick-freezing equipment for freezing lively fresh products |
| CN110542259A (en) * | 2019-08-09 | 2019-12-06 | 河南中镜科仪科技有限公司 | Quick freezing device |
| CN110736568A (en) * | 2019-11-05 | 2020-01-31 | 重庆市计量质量检测研究院 | Device and method for freezing large-scale water three-phase point bottle ice sleeve |
-
1994
- 1994-12-21 CN CN 94248681 patent/CN2208205Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103335478A (en) * | 2013-07-25 | 2013-10-02 | 钦龙金属工业（昆山）有限公司 | Lifting frame for lifting type liquid refrigerating equipment |
| CN103340467A (en) * | 2013-07-25 | 2013-10-09 | 钦龙金属工业（昆山）有限公司 | Quick-freezing equipment for freezing lively fresh products |
| CN103340467B (en) * | 2013-07-25 | 2014-09-24 | 钦龙金属工业（昆山）有限公司 | Quick-freezing equipment for freezing lively fresh products |
| CN110542259A (en) * | 2019-08-09 | 2019-12-06 | 河南中镜科仪科技有限公司 | Quick freezing device |
| CN110736568A (en) * | 2019-11-05 | 2020-01-31 | 重庆市计量质量检测研究院 | Device and method for freezing large-scale water three-phase point bottle ice sleeve |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |