CN106480139A - The method of comprehensive utilization of fish scale - Google Patents
The method of comprehensive utilization of fish scale Download PDFInfo
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- CN106480139A CN106480139A CN201510550749.8A CN201510550749A CN106480139A CN 106480139 A CN106480139 A CN 106480139A CN 201510550749 A CN201510550749 A CN 201510550749A CN 106480139 A CN106480139 A CN 106480139A
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Abstract
The invention discloses the method for comprehensive utilization of fish scale.Fish scale is the side-product after fish processing, abundant collagen protein and fish scale keratin is contained in fish scale, the present invention is by wearing into slurry fish scale, bacillus subtilises are prepared into fermentation medium, again fish scale slurry is fermented together with fermentation medium, after fermentation liquid centrifugation, lyophilization, extract collagen peptide, produce activated carbon using being extracted the residue after collagen peptide further, effectively the extracts active ingredients being present in fish scale out, turn waste into wealth, extract in separation process whole, no organic waste liquid is discharged, pollution-free, no slag and effluent is discharged, product property is stable, it is effectively utilized resource, reduce product cost, on the other hand decrease environmental pollution, increased the competitiveness of product.
Description
Technical field
The present invention relates to the method for comprehensive utilization of fish scale, belong to marine product technical field of comprehensive utilization.
Background technology
Substantial amounts of fish scale can be left, fish scale accounts for the 1-5% of fish body weight, containing rich in protein, lecithin and several mineral materials after fish processing；Wherein Organic substance accounts for 41%～55%, and protein accounts for the 70% of total organic matter, predominantly collagen protein and fish scale keratin,, in immunogenicity, biocompatibility, mechanical performance, biodegradability, the property digested and assimilated, moisture retention, antibiotic property, antioxidant, the character of the aspect such as safety is excellent for it；Secondly the inorganic elementss such as substantial amounts of calcium and phosphorus are also contained.However, the traditional processing method of fish scale be dried after as production feedstuff raw material, but in the dry run preparing feedstuff, the foul odour of effusion causes to environment greatly to pollute.These beneficial containing in fish scale are not used, and really greatly waste.Therefore, extract active ingredient from fish scale, become more meaningful.Extract active ingredient and be primarily directed to fish scale collagen and calcium, phosphorus composition in fish scale.At present, the extracting method of collagen peptide has acid extraction method, alkali extraction method, enzyme extraction method, combined extracting method etc..And the present invention proposes the new method being different from these traditional methods, meanwhile, additionally provide the Application way of the remnant after being extracted collagen peptide, further increasing the availability of resource.
Content of the invention
The purpose of the present invention, it is simply that providing a kind of method of comprehensive utilization of fish scale, is extracted collagen peptide from fish scale using fermentation method, and is further activated to being extracted the remnant after collagen peptide, prepare activated carbon.Overcome the shortcomings of, existing for traditional extracting method, to make full use of resource.
For achieving the above object, present invention employs such measure：The method of comprehensive utilization of fish scale, comprises the steps：
（1）Fish scale after fish is processed is cleaned, and is 1 with solid-liquid weight ratio：The ratio of 1-1.2 adds water, pulp of regrinding, and obtains fish scale slurry standby：
（2）By bacillus subtilises（Bacillus
subtilis subsp subtilis）It is inoculated in culture medium, every strain inoculates 5 culture medium, culture medium is nutrient agar culture medium, constant volume is 1000 milliliters, cultivate 50-65 hour under the conditions of 26-35 DEG C；
（3）Take above-mentioned cultured bacteria culture fluid 6-10 milliliter to add in 30-50 kilogram of fish scale slurry to stir, 12-18 hour of fermenting under conditions of 36-40 DEG C, period timing agitation starches uniform fermentation by fish scale；
（4）The fish scale fermenting is starched and is separated in rotating speed is for 2000-3000 rev/min of centrifugal separator, obtains fermentation liquid and fish scale residue is standby；
（5）Fermentation liquid is concentrated in vacuo to 50 DEG C of heat under the conditions of 40-50 DEG C and surveys relative density is 1.12-1.15, obtains concentrated solution, then concentrated solution lyophilization is obtained collagen peptide；
（6）Take above-mentioned fish scale residue to be dried under the conditions of 90-100 DEG C, compare 1 by solid-liquid weight：The ratio of 0.5-2.0, fish scale residue is added mix homogeneously in phosphoric acid, impregnates 6-12 hour, taking-up drains, and puts into and is warming up to 450-750 DEG C of activation in tube furnace, is incubated 30-60 minute, cooling, being rinsed to pH value with water is 6.5-7.0, is dried and obtains final product activated carbon to water content 3-8%.
Described bacillus subtilises Bacillus
subtilis subsp subtilisExisting merchandise sales, bacillus subtilises used by the present invention are bought from Institute of Microorganism, Academia Sinica.
Take the present invention of above-mentioned measure, take full advantage of remaining fish scale after fish processing, comprehensively utilized, extract collagen peptide especially with fermentation method, produce activated carbon using being extracted the residue after collagen peptide further, effectively extract being present in fish scale active ingredient, turn waste into wealth, extract in separation process whole, no organic waste liquid is discharged, pollution-free, no slag and effluent is discharged, and product property is stable, it is effectively utilized resource, reduce product cost, on the other hand decrease environmental pollution, increased the competitiveness of product.
For describing the present invention in detail, with reference to embodiment, the present invention is described in further detail：
Embodiment 1
（1）Take 100 kilograms of fish scales to clean, add 100 kg of water, in fiberizer, wear into pulpous state, obtain fish scale slurry standby：
（2）Carry out spawn culture, take bacillus subtilises 1, be inoculated in nutrient agar culture medium, nutrient agar culture medium is mainly made up of following raw materials：10 grams of peptone, 3 grams of Carnis Bovis seu Bubali cream, 5 grams of agar, 5 grams of sodium chloride plus sterilized water are to 1000 milliliters, and adjust PH to 7, and the culture medium that above-mentioned raw materials are configured to is divided into 5, routinely Spawn incubation method is cultivated 50 hours under the conditions of 35 DEG C, is then combined with cultivated strain and obtains bacteria culture fluid；
（3）Take in above-mentioned 50 kilograms of fish scales slurries of 10 milliliters of additions of cultured bacteria culture fluid and stir, ferment 18 hours under conditions of 36 DEG C, stirring in every 2 hours once, allows fish scale starch uniform fermentation, the fish scale fermenting is starched under conditions of being placed in 5 DEG C and stablized after-ripening 120 hours；
（4）The fish scale fermenting starch in rotating speed be 3000 revs/min centrifugal separator in separate, obtain fermentation liquid and fish scale residue；
（5）Taking zymotic fluid be concentrated in vacuo to 50 DEG C of heat under the conditions of 40-50 DEG C to survey relative density is 1.15, obtains concentrated solution, then concentrated solution lyophilization is obtained collagen peptide；
（6）Take above-mentioned fish scale residue to be dried under the conditions of 90-100 DEG C, compare 1 by solid-liquid weight：2.0 ratio, fish scale residue is added mix homogeneously in phosphoric acid, impregnates 6 hours, taking-up drains, and puts into and is warming up to 750 DEG C of activation in tube furnace, is incubated 60 minutes, cooling, and being rinsed to pH value with water is 6.5-7.0, is dried and obtains activated carbon to water content 3-8%.
Embodiment 2
（1）Take 100 kilograms of fish scales to clean, add 120 kg water, in fiberizer, wear into pulpous state, obtain fish scale slurry standby：
（2）Carry out spawn culture, take bacillus subtilises 1, be inoculated in nutrient agar culture medium, nutrient agar culture medium is mainly made up of following raw materials：10 grams of peptone, 3 grams of Carnis Bovis seu Bubali cream, 5 grams of agar, 5 grams of sodium chloride plus sterilized water are to 1000 milliliters, and adjust PH to 7, and the culture medium that above-mentioned raw materials are configured to is divided into 5, routinely Spawn incubation method is cultivated 65 hours under the conditions of 26 DEG C, is then combined with cultivated strain and obtains bacteria culture fluid；
（3）Take in above-mentioned 30 kilograms of fish scales slurries of 6 milliliters of additions of cultured bacteria culture fluid and stir, ferment 12 hours under conditions of 40 DEG C, stirring in every 2 hours once, allows fish scale starch uniform fermentation, the fish scale fermenting is starched under conditions of being placed in 10 DEG C and stablized after-ripening 100 hours；
（4）The fish scale fermenting starch in rotating speed be 2000 revs/min centrifugal separator in separate, obtain fermentation liquid and fish scale residue；
（5）Taking zymotic fluid be concentrated in vacuo to 50 DEG C of heat under the conditions of 40-50 DEG C to survey relative density is 1.12, obtains concentrated solution, then concentrated solution lyophilization is obtained collagen peptide；
（6）Take above-mentioned fish scale residue to be dried under the conditions of 90-100 DEG C, compare 1 by solid-liquid weight：1.0 ratio, fish scale residue is added mix homogeneously in phosphoric acid, impregnates 12 hours, taking-up drains, and puts into and is warming up to 450 DEG C of activation in tube furnace, is incubated 30 minutes, cooling, and being rinsed to pH value with water is 6.5-7.0, is dried and obtains activated carbon to water content 3-8%.
Claims (1)
1. the method for comprehensive utilization of fish scale is it is characterised in that methods described comprises the steps：
（1）Fish scale after fish is processed is cleaned, and is 1 with solid-liquid weight ratio：The ratio of 1-1.2 adds water, pulp of regrinding, and obtains fish scale slurry standby：
（2）By bacillus subtilises（Bacillus subtilis subsp subtilis）It is inoculated in culture medium, every strain inoculates 5 culture medium, culture medium is nutrient agar culture medium, constant volume is 1000 milliliters, cultivate 50-65 hour under the conditions of 26-35 DEG C；
（3）Take above-mentioned cultured bacteria culture fluid 6-10 milliliter to add in 30-50 kilogram of fish scale slurry to stir, 12-18 hour of fermenting under conditions of 36-40 DEG C, period timing agitation starches uniform fermentation by fish scale；
（4）The fish scale fermenting is starched and is separated in rotating speed is for 2000-3000 rev/min of centrifugal separator, obtains fermentation liquid and fish scale residue is standby；
（5）Fermentation liquid is concentrated in vacuo to 50 DEG C of heat under the conditions of 40-50 DEG C and surveys relative density is 1.12-1.15, obtains concentrated solution, then concentrated solution lyophilization is obtained collagen peptide；
（6）Take above-mentioned fish scale residue to be dried under the conditions of 90-100 DEG C, compare 1 by solid-liquid weight：The ratio of 0.5-2.0, fish scale residue is added mix homogeneously in phosphoric acid, impregnates 6-12 hour, taking-up drains, and puts into and is warming up to 450-750 DEG C of activation in tube furnace, is incubated 30-60 minute, cooling, being rinsed to pH value with water is 6.5-7.0, is dried and obtains final product activated carbon to water content 3-8%.
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| CN201510550749.8A CN106480139A (en) | 2015-09-01 | 2015-09-01 | The method of comprehensive utilization of fish scale |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240313A (en) * | 2008-03-07 | 2008-08-13 | 陈成 | Method for preparing collagen peptide |
| CN101886106A (en) * | 2010-07-02 | 2010-11-17 | 湖北远成药业有限公司 | Method for extracting collagen peptide from fish scales |
| CN102199646A (en) * | 2011-03-15 | 2011-09-28 | 广东海洋大学 | Method for preparing collagen peptide with fish skin |
| CN103991870A (en) * | 2014-05-09 | 2014-08-20 | 山东大学 | Method for preparing active carbon from fish scale |
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2015
- 2015-09-01 CN CN201510550749.8A patent/CN106480139A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240313A (en) * | 2008-03-07 | 2008-08-13 | 陈成 | Method for preparing collagen peptide |
| CN101886106A (en) * | 2010-07-02 | 2010-11-17 | 湖北远成药业有限公司 | Method for extracting collagen peptide from fish scales |
| CN102199646A (en) * | 2011-03-15 | 2011-09-28 | 广东海洋大学 | Method for preparing collagen peptide with fish skin |
| CN103991870A (en) * | 2014-05-09 | 2014-08-20 | 山东大学 | Method for preparing active carbon from fish scale |
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