CN105331643B - A method of Pfansteihl is prepared using sophora bud dregs of a decoction fermentation - Google Patents
A method of Pfansteihl is prepared using sophora bud dregs of a decoction fermentation Download PDFInfo
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Abstract
A method of Pfansteihl is prepared using sophora bud dregs of a decoction fermentation, comprising the following steps: (1) is 2-5wt% by dregs of a decoction drying to water content；(2) it by the dregs of a decoction and 50 parts of wheat bran, 0.4-0.6 parts of corn flour, 5-7 parts of ammonium chloride, 3-5 parts of potassium dihydrogen phosphate, is uniformly mixed in 25-40 parts of sterile water, natural pH, high pressure sterilization obtains dregs of a decoction culture medium；(3) first order seed of Rhizopus oryzae strain, 25-30 DEG C of fermentation 24-96h are accessed in dregs of a decoction culture medium, culture medium is shaken to mixed uniformly by average every 12h that crosses；(4) with the dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate is obtained；Add milk of lime to adjust pH9.5-10, clarifies to obtain supernatant；(5) supernatant is concentrated under reduced pressure, the crystallized precipitation calcium lactate of concentrated liquid；(6) mother liquor is filtered, is placed in 100 DEG C of baking ovens and dries 2-2.5h, at powdered；(7) heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification is added, and filters to obtain crude lactic acid.This method simple process, it is time-consuming short, material consumption is reduced, is conducive to industry test and amplifies, and yield is higher.
Description
Technical field
The present invention relates to a kind of methods for preparing Pfansteihl using sophora bud dregs of a decoction fermentation, belong to technical field of biochemical industry.
Background technique
With the development of science and technology and progressive, Chinese herbal medicine becomes current deep processing from past simple processing, several by the past
Plant simple dosage form such as: pulvis, decoction, paste development are a variety of dosage forms such as oral agents, injection, capsule.China's Chinese medicine
Slag year discharge enormous amount not only pollutes environment, Er Qiezao at present to Chinese medicine slag processing mode based on accumulating, burn, fill
At the waste of resource.Advocating energy-saving and emission-reduction, the modern society of low-carbon environment-friendly is comprehensive exploitation natural resources of Chinese medicinal materials, is made full use of
The medicine dregs of a decoction provide thinking, further investigate Chinese medicine dreg purposes, the dregs of a decoction can be made to obtain deeper utilization.
Lactic acid (Lactic Acid), scientific name are α-hydracrylate (α-Hydroxy-Propanoic-Acid), and molecular formula is
C3H6O3, structural formula CH3CHOHCOOH, relative molecular mass 90.08 are a kind of common natural organic acids.In lactic acid molecules
Containing an asymmetric carbon atom, there is enantiomerism, there are two kinds of configurations of D type and L-type, wherein Pfansteihl is dextrorotation, D-
Lactic acid is left-handed.
In China, with the continuous increase expanded with consumption figure of lactic acid application field, the demand of lactic acid
Amount is also continuously increased, and only beer industry needs 3000 tons of lactic acid or more every year, and food, medical industry are also more next to the demand of lactic acid
It is bigger.The most important purposes of Pfansteihl is that polymerization generates polylactic acid, it has good compatibility and biodegradable, extensively
It is general to be applied to packing business, pharmaceuticals industry and textile industry.Production by Microorganism Fermentation lactic acid can pass through the choosing of strain and condition of culture
Select the Pfansteihl for obtaining that there is specificity.Fermentation method produce lactic acid except can using the monosaccharide such as glucose, lactose as raw material in addition to, moreover it is possible to
Lactic acid is produced using starch, cellulose as fermenting raw materials, therefore, Production by Microorganism Fermentation lactic acid is produced because its raw material sources is extensive
The advantages that product optical purity is high, highly-safe.Bacterial fermentation and root arrhizus fermentation can be divided into according to the type of fermentative microorganism.Seek
More cheap raw material is an important channel for reducing cost, while saving grain, alleviates people and animals and strives grain problem.Lactic acid conduct
Greening platform compound, raw materials for production should be green non-poisonous.Reproducible biomass resource is ideal production of lactic acid raw material,
Therefore discarded raw material of the Chinese medicine slag as lactic acid is studied, not only opens up the frontier of lactic biological matter raw material, but also look forward to for Chinese medicine
Industry industrial upgrading and structural adjustment provide a new thinking.
The sophora bud is conventional Chinese medicine, is the dry flower of leguminous plant Chinese scholartree, and Shennong's Herbal is classified as top grade, bitter,
It is slightly cold, there is cooling blood and hemostasis, clearing liver-fire and other effects.Recent domestic medical personal, which acts on it, to be studied, discovery
Its effective component has anti-cancer and cancer-preventing, platelet aggregation-against, analgesia, antibacterial, antiviral, anti-aging, anti-inflammatory, antiallergy, anti-freedom
Base and the various actives such as anti-oxidant, to improve myocardial circulation, clearing heat and detoxicating, reducing blood lipid, softening blood vessel, anti-inflammatory kidney tonifying etc. have it is good
Preventive and therapeutic effect, it is nontoxic to the human body, harmless, without lethal, carcinogenic, teratogenesis, mutagenesis.The wherein highest effective component of content
It is rutin (also known as rutin), is the main indicator for measuring sophora bud quality.Rutin Bei Shui Xie Cheng Quercetin skin under the action of enzyme or acid
Element.
It is the side that raw material low-cost high-efficiency produces lactic acid that patent document CN102586348A, which discloses a kind of lignocellulosic,
Method.The Pediococcus acidilactici for specifically separating and saving from lignocellulosic, Liquid Culture Pediococcus acidilactici obtain as kind
The cell thallus of son, is inoculated in the reactor of high wood fibre cellulose content, and cellulase is added, synchronizes saccharification and high temperature
Fermentation generates lactic acid；Or hydrolyzate of the cellulase saccharification acquisition containing sugar of lignocellulosic is carried out first, then use hydrolyzate
It carries out high temperature lactic fermentation and obtains lactic acid；Or Pediococcus acidilactici and lignocellulosic material mix, and carry out solid state rheology first,
Then fermentor is added in the lignocellulosic material for including Pediococcus acidilactici again, cellulase is added, realize synchronous saccharification
With hot fermentation lactic acid producing.The present invention uses unique zymotechnique and microorganism, and product design is high, process is simple, at low cost
It is honest and clean, especially using lignocellulosic as raw material, there is wide production space and development prospect.
Patent document CN1843152 discloses the method for the open type fermented production lactic acid and feed of kitchen garbage, feature
Through the following steps that carrying out the open type fermented production lactic acid of kitchen garbage: kitchen garbage is filtered dry, crushes by (one)；(2) to quilt
The water of 1~3 times of solid kitchen garbage quality is added in the solid kitchen garbage of crushing；(3) add into solid-liquid mixing kitchen garbage
Enter cellulase and amylase, by be added in every 100g solid-liquid mixing kitchen garbage 10~20kU of cellulase and amylase 5~
The ratio of 10kU is added；(4) between the solid-liquid mixing kitchen garbage of addition cellulase and amylase is under conditions of 35~50 DEG C
Disconnected property stirs open type fermented 20~30h, and it is 5~6 that calcium carbonate, which is added, to keep solid-liquid mixing kitchen garbage pH value；(5) solid-liquid
Separation, then the lactic acid in filtering fermentating liquid is purified to get lactic acid is arrived.
Patent document CN103892045A, which is disclosed, a kind of utilizes sophora bud solid dregs of a decoction production animal and fowl fodder and its preparation side
Method；Technical scheme applied to solve the technical problem are as follows: glucose, (NH is added4)2SO4、KH2PO4And MgSO4·7H2O, will be upper
It states after ingredient mixes and be dissolved in water according to a certain percentage, admixes in the sophora bud dregs of a decoction, add water and mix well, be put into culture plate
In, aspergillus niger is accessed, is compacted and shading, thermal-insulating sealing culture 3-5 days is dried in the environment of 40-50 DEG C to get leaven
Kind, it collects and stores；By the sophora bud dregs of a decoction by the method for biofermentation so that it becomes livestock feed solves dregs of a decoction discharge dirt
The problem of environment is contaminated, sophora bud dregs of a decoction livestock feed has broad application prospects.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for preparing Pfansteihl using sophora bud dregs of a decoction fermentation, using aerobic solid-state
Fermentation technique has opened up new raw material source for production of lactic acid.
To achieve the above object, the invention adopts the following technical scheme:
A method of Pfansteihl being prepared using sophora bud dregs of a decoction fermentation, method includes the following steps:
(1) pretreatment of the sophora bud dregs of a decoction: being 2-5wt% by dregs of a decoction drying to water content；
(2) preparation of dregs of a decoction culture medium: by the dregs of a decoction and 50 parts of wheat bran (parts by weight), Chinese medicine slag: wheat bran=1: 6-7: 1；
It 0.4-0.6 parts of corn flour, 5-7 parts of ammonium chloride, 3-5 parts of potassium dihydrogen phosphate, is uniformly mixed in 25-40 parts of sterile water, it is natural
PH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min；
(3) dregs of a decoction ferment: the first order seed of the Rhizopus oryzae strain of 5-30mL, 25- are accessed in every 100g dregs of a decoction culture medium
30 DEG C of fermentation 24-96h, average every 12h that crosses rock culture medium uniformly；
(4) product treatment: with the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate is obtained；Add into filtrate
Enter milk of lime and adjust pH9.5-10, clarifies to obtain supernatant；
(5) supernatant being concentrated under reduced pressure, temperature is 45-50 DEG C, it obtains concentrated liquid and is put into 3-5 DEG C of refrigerator, crystallization analysis
Calcium lactate out；
(6) mother liquor is filtered, is placed in 100 DEG C of baking ovens and dries 2-2.5h, at powdered；
(7) heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification is added, contained in the additional amount and crystal of sulfuric acid
Calcium molar fraction is identical, filters to obtain crude lactic acid.
Wherein, during clarification obtains supernatant in the step (4), the temperature of system is controlled at 52-57 DEG C.
The first order seed strain concentration (spore concentration) of the Rhizopus oryzae strain is (0.10-1.00) × 107A/mL.
The cultural method of the first order seed of the Rhizopus oryzae strain are as follows: by Rhizopus oryzae strain in PDA solid medium, 28
DEG C, it cultivates 5-7 days, obtains the first order seed of Rhizopus oryzae strain.
The ingredient of the PDA solid medium is potato 200g, glucose 20g, agar 15-20g, tap water
1000mL, natural pH.
The present invention has the advantages that
The present invention carries out aerobic fermentation to the sophora bud dregs of a decoction using Rhizopus oryzae strain, has opened up new raw material for production of lactic acid
The consumption of the traditional grains raw materials such as sweet potato, corn is reduced in source；And zymotechnique of the invention is simple, at low cost, process stabilizing.
The present invention not only can bring considerable economic benefit for the pharmaceutical factory of traditional Chinese medicine, but also reduce garbage loading embeading intensity, be conducive to improve air,
It is an important channel for reducing cost that water quality and soil living environment, which are cheap raw material with the discarded dregs of a decoction, while saving grain
Food alleviates people and animals and strives grain problem.
Specific embodiment
Below by way of specific embodiment, the present invention will be further described.
Reagent used in embodiment is SILVER REAGENT；The sophora bud dregs of a decoction are the resultant products of industrial abstract rutin.In work
Industry is extracted in the technique of rutin, and the sophora bud passes through boiling, and filtered solid is the sophora bud dregs of a decoction, and liquid crystallisation by cooling obtains reed
Fourth.Rhizopus oryzae strain derives from China General Microbiological culture presevation administrative center (CGMCC), and the deposit number of Rhizopus oryzae is
3.819。
The preparation process of PDA solid medium employed in embodiment are as follows: potato is first cleaned into peeling, weighs 300g
Potato is cut into small pieces, and adds boiling rotten, with four layers of filtered through gauze, adds glucose 30g and agar 30g, continues heating stirring and mixes,
Moisture is supplied after slightly cooling again to 1000mL, dispenses, jump a queue, wrap up, taken out after 115 DEG C of sterilizing 30min test tube put inclined-plane or
It shakes up, is stored after cooling spare.
Embodiment 1
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh.Weigh sophora bud dregs of a decoction 10g, wheat bran
10g, corn flour 0.18g, ammonium chloride 2.0g, potassium dihydrogen phosphate 2.0g, are dissolved in the sterile water of 16g, and mixed liquor obtained is fallen
Enter in conical flask and stir and evenly mix, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.It is trained in every 100g dregs of a decoction
The first order seed that the Rhizopus oryzae strain of 25mL is accessed in base is supported, dilution spore concentration is 9.3 × 106A/mL, 28 DEG C of fermentation 48h,
Average every 12h that crosses rocks culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate lactic acid is obtained
Content is 1.450mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.55 DEG C of heat preservation is needed in clarifying process；It will
Supernatant is concentrated under reduced pressure, and temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.Mother liquor is filtered,
It is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification, sulfuric acid is added
Additional amount it is identical as institute's calcic molar fraction in crystal, filter to obtain crude lactic acid 5.85mg.
Embodiment 2
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 14g；Wheat bran
7g, corn flour 0.18g, ammonium chloride 2.1g, potassium dihydrogen phosphate 2.1g are dissolved in the sterile water of 15.8g, and mixed liquor obtained is fallen
Enter in conical flask and stir and evenly mix, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.It is trained in every 100g dregs of a decoction
The first order seed that the Rhizopus oryzae strain of 20mL is accessed in base is supported, dilution spore concentration is 9.3 × 106A/mL, 26 DEG C of fermentation 96h,
Average every 12h that crosses rocks culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate lactic acid is obtained
Content is 1.144mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.55 DEG C of heat preservation is needed in clarifying process；It will
Supernatant is concentrated under reduced pressure, and temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.Mother liquor is filtered,
It is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification, sulfuric acid is added
Additional amount it is identical as institute's calcic molar fraction in crystal, filter to obtain crude lactic acid 5.49mg.
Embodiment 3
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 15g；Wheat bran
5g, corn flour 0.18g, ammonium chloride 2.0g, potassium dihydrogen phosphate 2.0g are dissolved in the sterile water of 16.0g, and mixed liquor obtained is fallen
Enter in conical flask and stir and evenly mix, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.It is trained in every 100g dregs of a decoction
The first order seed that the Rhizopus oryzae strain of 10mL is accessed in base is supported, dilution spore concentration is 9.3 × 106A/mL, 30 DEG C of fermentation 48h,
Average every 12h that crosses rocks culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate lactic acid is obtained
Content is 2.066mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.55 DEG C of heat preservation is needed in clarifying process；It will
Supernatant is concentrated under reduced pressure, and temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.Mother liquor is filtered,
It is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification, sulfuric acid is added
Additional amount it is identical as institute's calcic molar fraction in crystal, filter to obtain crude lactic acid 8.68mg.
Embodiment 4
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 16g；Wheat bran
4g, corn flour 0.18g, ammonium chloride 2.4g, potassium dihydrogen phosphate 2.4g are dissolved in the sterile water of 15.2g, and mixed liquor obtained is fallen
Enter in conical flask and stir and evenly mix, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.It is trained in every 100g dregs of a decoction
The first order seed that the Rhizopus oryzae strain of 5mL is accessed in base is supported, dilution spore concentration is 9.3 × 106A/mL, 27 DEG C of fermentation 96h,
Average every 12h that crosses rocks culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate lactic acid is obtained
Content is 1.320mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.55 DEG C of heat preservation is needed in clarifying process；It will
Supernatant is concentrated under reduced pressure, and temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.Mother liquor is filtered,
It is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification, sulfuric acid is added
Additional amount it is identical as institute's calcic molar fraction in crystal, filter to obtain crude lactic acid 6.07mg.
Embodiment 5
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 20g；Wheat bran
4g, corn flour 0.18g, ammonium chloride 2.4g, potassium dihydrogen phosphate 2.4g are dissolved in the sterile water of 15.2g, and mixed liquor obtained is fallen
Enter in conical flask and stir and evenly mix, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.It is trained in every 100g dregs of a decoction
The first order seed that the Rhizopus oryzae strain of 30mL is accessed in base is supported, dilution spore concentration is 9.3 × 106A/mL, 25 DEG C of fermentation 30h,
Average every 12h that crosses rocks culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate lactic acid is obtained
Content is 1.630mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.55 DEG C of heat preservation is needed in clarifying process；It will
Supernatant is concentrated under reduced pressure, and temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.Mother liquor is filtered,
It is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification, sulfuric acid is added
Additional amount it is identical as institute's calcic molar fraction in crystal, filter to obtain crude lactic acid 7.83mg.
Embodiment 6
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 18g；Wheat bran
3g, corn flour 0.18g, ammonium chloride 2.1g, potassium dihydrogen phosphate 2.1g are dissolved in the sterile water of 15.8g, and mixed liquor obtained is fallen
Enter in conical flask and stir and evenly mix, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.It is trained in every 100g dregs of a decoction
The first order seed that the Rhizopus oryzae strain of 20mL is accessed in base is supported, dilution spore concentration is 9.3 × 106A/mL, 29 DEG C of fermentation 72h,
Average every 12h that crosses rocks culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate lactic acid is obtained
Content is 1.168mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.55 DEG C of heat preservation is needed in clarifying process；It will
Supernatant is concentrated under reduced pressure, and temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.Mother liquor is filtered,
It is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification, sulfuric acid is added
Additional amount it is identical as institute's calcic molar fraction in crystal, filter to obtain crude lactic acid 5.61mg.
Embodiment 7
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 21g；Wheat bran
3g, corn flour 0.18g, ammonium chloride 2.4g, potassium dihydrogen phosphate 2.4g are dissolved in the sterile water of 15.2g, and mixed liquor obtained is fallen
Enter in conical flask and stir and evenly mix, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.It is trained in every 100g dregs of a decoction
The first order seed that the Rhizopus oryzae strain of 15mL is accessed in base is supported, dilution spore concentration is 9.3 × 106A/mL, 26 DEG C of fermentation 30h,
Average every 12h that crosses rocks culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate lactic acid is obtained
Content is 1.624mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.55 DEG C of heat preservation is needed in clarifying process；It will
Supernatant is concentrated under reduced pressure, and temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.Mother liquor is filtered,
It is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification, sulfuric acid is added
Additional amount it is identical as institute's calcic molar fraction in crystal, filter to obtain crude lactic acid 7.80mg.
Embodiment 8
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 7g；Wheat bran
14g, corn flour 0.18g, ammonium chloride 2.1g, potassium dihydrogen phosphate 2.1g are dissolved in the sterile water of 15.8g, by mixed liquor obtained
It pours into conical flask and stirs and evenly mixs, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.In every 100g dregs of a decoction
The first order seed of the Rhizopus oryzae strain of 5mL is accessed in culture medium, dilution spore concentration is 9.3 × 106A/mL, 30 DEG C of fermentations
72h, average every 12h that crosses rock culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate is obtained
Lactic acid content is 1.545mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.Heat preservation 55 is needed in clarifying process
℃；Supernatant is concentrated under reduced pressure, temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.It filters
Mother liquor is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acid is added
Change, the additional amount of sulfuric acid is identical as institute's calcic molar fraction in crystal, filters to obtain crude lactic acid 7.41mg.
Embodiment 9
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 5g；Wheat bran
15g, corn flour 0.18g, ammonium chloride 2.0g, potassium dihydrogen phosphate 2.0g are dissolved in the sterile water of 16.0g, by mixed liquor obtained
It pours into conical flask and stirs and evenly mixs, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.In every 100g dregs of a decoction
The first order seed of the Rhizopus oryzae strain of 30mL is accessed in culture medium, dilution spore concentration is 9.3 × 106A/mL, 28 DEG C of fermentations
For 24 hours, average every 12h that crosses rocks culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate is obtained
Lactic acid content is 2.504mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.Heat preservation 55 is needed in clarifying process
℃；Supernatant is concentrated under reduced pressure, temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.It filters
Mother liquor is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acid is added
Change, the additional amount of sulfuric acid is identical as institute's calcic molar fraction in crystal, filters to obtain crude lactic acid 12.02mg.
Embodiment 10
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 4g；Wheat bran
16g, corn flour 0.18g, ammonium chloride 2.1g, potassium dihydrogen phosphate 2.1g are dissolved in the sterile water of 15.8g, by mixed liquor obtained
It pours into conical flask and stirs and evenly mixs, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.In every 100g dregs of a decoction
The first order seed of the Rhizopus oryzae strain of 25mL is accessed in culture medium, dilution spore concentration is 9.3 × 106A/mL, 25 DEG C of fermentations
54h, average every 12h that crosses rock culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate is obtained
Lactic acid content is 1.606mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.Heat preservation 55 is needed in clarifying process
℃；Supernatant is concentrated under reduced pressure, temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.It filters
Mother liquor is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acid is added
Change, the additional amount of sulfuric acid is identical as institute's calcic molar fraction in crystal, filters to obtain crude lactic acid 7.71mg.
Embodiment 11
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 4g；Wheat bran
20g, corn flour 0.18g, ammonium chloride 2.1g, potassium dihydrogen phosphate 2.1g are dissolved in the sterile water of 15.8g, by mixed liquor obtained
It pours into conical flask and stirs and evenly mixs, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.In every 100g dregs of a decoction
The first order seed of the Rhizopus oryzae strain of 15mL is accessed in culture medium, dilution spore concentration is 9.3 × 106A/mL, 29 DEG C of fermentations
For 24 hours, average every 12h that crosses rocks culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate is obtained
Lactic acid content is 1.391mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.Heat preservation 55 is needed in clarifying process
℃；Supernatant is concentrated under reduced pressure, temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.It filters
Mother liquor is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acid is added
Change, the additional amount of sulfuric acid is identical as institute's calcic molar fraction in crystal, filters to obtain crude lactic acid 6.68mg.
Embodiment 12
It is 3% by sophora bud dregs of a decoction drying to water content, crushes, powder size is 10 mesh, weighs sophora bud dregs of a decoction 3g；Wheat bran
18g, corn flour 0.18g, ammonium chloride 2.1g, potassium dihydrogen phosphate 2.1g are dissolved in the sterile water of 15.8g, by mixed liquor obtained
It pours into conical flask and stirs and evenly mixs, natural pH obtains dregs of a decoction culture medium in 121 DEG C of high pressure sterilization 20min.In every 100g dregs of a decoction
The first order seed of the Rhizopus oryzae strain of 10mL is accessed in culture medium, dilution spore concentration is 9.3 × 106A/mL, 27 DEG C of fermentations
54h, average every 12h that crosses rock culture medium uniformly.With the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate is obtained
Lactic acid content is 2.002mg/g；Add milk of lime (adjusting pH9.5-10), centrifugation obtains supernatant.Heat preservation 55 is needed in clarifying process
℃；Supernatant is concentrated under reduced pressure, temperature is 50 DEG C, obtains concentrated liquid and is put into 4 DEG C of refrigerators, and calcium lactate is precipitated in crystallization.It filters
Mother liquor is placed in 100 DEG C of baking ovens and dries 2h, at powdered.Heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acid is added
Change, the additional amount of sulfuric acid is identical as institute's calcic molar fraction in crystal, filters to obtain crude lactic acid 9.61mg.
Claims (4)
1. a kind of method for preparing Pfansteihl using sophora bud dregs of a decoction fermentation, which is characterized in that method includes the following steps:
(1) pretreatment of the sophora bud dregs of a decoction: being 2-5wt% by dregs of a decoction drying to water content；
(2) preparation of dregs of a decoction culture medium: by the dregs of a decoction and 50 parts of wheat bran, Chinese medicine slag: wheat bran=1:6-7:1；Corn flour 0.4-0.6
Part, it 5-7 parts of ammonium chloride, 3-5 parts of potassium dihydrogen phosphate, is uniformly mixed in 25-40 parts of sterile water, natural pH, in 121 DEG C of high pressures
Sterilize 20min, obtains sophora bud dregs of a decoction culture medium；
(3) dregs of a decoction ferment: the first order seed of the Rhizopus oryzae strain of 5-30mL, the rice root are accessed in every 100g dregs of a decoction culture medium
The first order seed strain concentration of mould species is (0.10-1.00) × 107A/mL；25-30 DEG C of fermentation 24-96h, it is averagely every to cross 12h
Culture medium is rocked uniformly；
(4) product treatment: with the solid dregs of a decoction culture medium obtained after distilled water elution fermentation, filtrate is obtained；Stone is added into filtrate
Grey cream adjusts pH 9.5-10, clarifies to obtain supernatant；
(5) supernatant is concentrated under reduced pressure, temperature is 45-50 DEG C, obtains concentrated liquid and is put into 3-5 DEG C of refrigerator, and cream is precipitated in crystallization
Sour calcium；
(6) mother liquor is filtered, is placed in 100 DEG C of baking ovens and dries 2-2.5h, at powdered；
(7) heating stirring makes calcium lactate dissolution of crystals, and concentrated sulfuric acid acidification is added, and institute's calcic rubs in the additional amount and crystal of sulfuric acid
Your score is identical, filters to obtain crude lactic acid.
2. the method according to claim 1 for preparing Pfansteihl using sophora bud dregs of a decoction fermentation, which is characterized in that the step
(4) during clarification obtains supernatant in, the temperature of system is controlled at 52-57 DEG C.
3. the method according to claim 1 for preparing Pfansteihl using sophora bud dregs of a decoction fermentation, which is characterized in that the rice root
The cultural method of the first order seed of mould species are as follows: by Rhizopus oryzae strain in PDA solid medium, 28 DEG C, cultivate 5-7 days, obtain
The first order seed of Rhizopus oryzae strain.
4. the method according to claim 3 for preparing Pfansteihl using sophora bud dregs of a decoction fermentation, which is characterized in that the PDA
The ingredient of solid medium is potato 200g, glucose 20g, agar 15-20g, water 1000mL, natural pH.
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