CN106478837A - A kind of can be used for green algae polysaccharide iron complexes of animals and plants iron supplement and preparation method thereof - Google Patents
A kind of can be used for green algae polysaccharide iron complexes of animals and plants iron supplement and preparation method thereof Download PDFInfo
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- CN106478837A CN106478837A CN201611056612.8A CN201611056612A CN106478837A CN 106478837 A CN106478837 A CN 106478837A CN 201611056612 A CN201611056612 A CN 201611056612A CN 106478837 A CN106478837 A CN 106478837A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention provides a kind of prepare the preparation method of green algae polysaccharide ferrum with chlorella and high price ferrum for raw material, belong to metal complex technical field.It is combined offer new raw material for producing polyferose.The green algae polysaccharide that the present invention provides, it is that sea grass polysaccharide is extracted from chlorella with the method for thermokalite water extraction, in pH 6.5 9.0 aqueous solution, Deca iron sulfate or ferric chloride solution preparation green algae polysaccharide iron complexes in green algae polysaccharide extracting solution, and purification is carried out using nanofiltration technique, this complex can be applicable to feed additive, bio-feritlizer etc..
Description
Technical field
The present invention relates to a kind of can be used for green algae polysaccharide iron complexes of animals and plants iron supplement and preparation method thereof and in particular to
A kind of with green algae polysaccharide and the Nu-Iron preparation method as raw material for the high price ferrum, belong to metal complex field.
Background technology
Ferrum is plant, animal and trace element needed by human.Ferrum to the synthesis of plant chlorophyll and promotes many enzymes
Activity be requisite, thus ferrum plays for the metabolism promoting the photosynthesis of plant, Repiration, matter and energy
Important effect.In animal and human body, ferrum is the constituent of hemoglobin, and hemoglobin participates in transport and the storage of oxygen.
Because the storage of internal ferrum can not meet the needs of normocyte generation, the anemia that occurs is referred to as iron deficiency anemia.Ferrum simultaneously
Necessary for enzymes various in body, it is also the necessary material of purine biosynthesis, directly participates in human energy metabolism, impact body is exempted from
Epidemic disease.
Inorganic iron preparation is easily oxidized or forms precipitation, especially in oxidation state and neutrality in basic soil, ion
The concentration of iron of state is very low, and the inorganic iron of supply is far below the needs of plant.Inorganic iron can produce thorn to body gastrointestinal tract simultaneously
Swash, easily cause the side effect such as constipation, nausea, diarrhoea, be also unfavorable for the absorption of animal and human body.On the other hand, inhale with regard to plant
Although the mechanism receiving ferrum has multiple sayings, its absorption process is as a whole, iron ion first some complexations with plant secretion
Agent（As citric acid）Form complex, root table is reached with complex pattern, is absorbed into intracellular, therefore chelated iron specific ionization ferrum is more
Be conducive to the absorption of plant.And absorption in animal body for the ferrum is also the form by complex, ferrous ion first have to
Vitamin C in intestinal contentses, some sugar and aminoacid form complex, then absorb in duodenum and jejunum.Therefore, iron supplement
Preparation also progressively develops into Organic Iron, Complexing Iron by original inorganic iron, and wherein polyferose is a kind of preferable iron supplementary, property
Stable, it is not easily decomposed at physiological ph, also will not be polymerized, and existed with soluble form, absorbance is high.
In Nu-Iron, the polysaccharide as part can be oligomeric glucose, Radix Angelicae Sinensis polysaccharide, red jujube polysaccharide etc.,
And the chlorella that polyoses content enriches is also a kind of preferable, cheap polysaccharide ligand origins.In algae, Chlorophyta is species
Most one, widely distributed Yu Haiyang, in lake and rivers, rich in polysaccharide, protein, fat, vitamin and mineral etc.,
Wherein water solublity sulfated polysaccharide content highest, common have two classes：Xylose-arabinose-galactan and glucuronic acid-wood
Sugar-rhamnosan.These polysaccharide generally have the biological activity of blood fat reducing, antioxidation, antiviral and enhancing immunity.Chlorella
Polysaccharide can also be absorbed by plants, and can strengthen the drought resisting of plant, cold-resistant, saline-alkaline tolerance.Chlorella is widely distributed, cheap, its
In contain a large amount of water soluble polysaccharide, have the biological activity of uniqueness, avirulence, it is former to be that part prepared by a kind of polyferose of rationality
Material.
Content of the invention
In order to overcome the technical deficiency of inorganic iron preparation in prior art, the present invention provides one kind to can be used for animals and plants iron supplement
Green algae polysaccharide iron complexes and preparation method thereof,.
For achieving the above object, technical solution of the present invention is as follows：Main process include extraction and the polysaccharide of green algae polysaccharide
With the complex reaction of high price ferrum, wherein
1）Green algae polysaccharide is extracted：After chlorella drying and crushing, add the water of 10-20 times of volume, tune pH is 9.0-12.0,60-80 DEG C
2-4 hour is extracted in lower stirring, and continuous 2-3 rear extracting solution of extraction merges, and obtains green algae polysaccharide using 750 mesh filter-cloth filterings and extracts
Liquid；
2）The preparation of green algae polysaccharide iron complexes：The pH being added thereto to citric acid regulation green algae polysaccharide extracting solution is 6.5-
9.0, solution is warming up to 70-90 DEG C, is slowly added dropwise iron chloride or the ferrum sulfuricum oxydatum solutum of 2mol/L thereto, until producing reddish brown
Color precipitates, and continues stirring reaction 2-6 hour at 70-90 DEG C, and reactant liquor is centrifuged 10-20 minute, supernatant under 3500rpm rotating speed
Liquid is crossed NF membrane and is obtained nanofiltration concentrate, and nanofiltration concentrate is spray-dried, and obtains final product green algae polysaccharide iron complexes.
The preparation method of green algae polysaccharide iron complexes as above, the molecular cut off of described NF membrane is 300-
500Da.Wherein said supernatant multiple can cross NF membrane, until concentrated solution electrical conductivity ＜ 30us/cm.
Green algae polysaccharide iron complexes described above can be used for preparing feed additive or bio-feritlizer.It can be used for
Animals and plants iron supplement.The impact experiment to spinach growth for the embodiment of the present invention 3 green algae polysaccharide shows, foliar spray green algae polysaccharide ferrum, EDTA
Ferrum sodium all can increase Spinach Leaf number, leaf color value, vane thickness and Herba Spinaciae dry weight in various degree, and wherein foliar spray iron fertilizer is increasing
Also there is preferable performance, the advantage of green algae polysaccharide ferrum becomes apparent from vane thickness and increase Herba Spinaciae dry weight.
The present invention compared with prior art, it is a technical advantage that：
1）Preparation-obtained green algae polysaccharide iron complexes overcome inorganic iron preparation technology that is easily oxidized or forming precipitation
Deficiency, and stimulation is produced to body gastrointestinal tract, easily cause the side effect such as constipation, nausea, diarrhoea, animals and plants iron supplement preferably makes
With.
2）Described green algae polysaccharide iron complexes preparation process is simple, raw material is easy to get, and is suitable for industrially applying on a large scale.
3）Described green algae polysaccharide iron complexes can strengthen the drought resisting of plant, cold-resistant, saline-alkaline tolerance, and have blood fat reducing, resist
The biological activity of oxidation, antiviral and enhancing immunity.
Brief description
Fig. 1 sea grass polysaccharide ferrum is applied to the impact to content of hemoglobin in the experiment of iron deficiency mice
Specific embodiment：
Embodiment 1：The preparation method of sea grass polysaccharide iron complexes
Took the Entermorpha dry powder 1kg of 60 mesh sieves, added 10L water, and adjusted pH 9.5, be warming up to 80 DEG C, stirring extraction 3 hours, repeatedly
Extract 2 times, united extraction liquid, be neutralized to neutrality using after 750 mesh filter cloth plate-and-frame filtration.Citric acid, stirring is added in filtrate
Dissolving, is warming up to 70 DEG C, keeps pH between 6.5-8.0, and Deca 2 mol/L liquor ferri trichloridi, sinks to producing brownish red
Till shallow lake, continue stirring reaction 2 hours at 70 DEG C.Reactant liquor is centrifuged 10 minutes under 3500rpm rotating speed, supernatant nanofiltration
Equipment desalination, the molecular cut off of described NF membrane is 300 Da, spray dried when nanofiltration concentrate electrical conductivity is 18us/cm
Dry, obtain sea grass polysaccharide iron complexes.
Embodiment 2：The preparation method of Codiumfragile(sur.) Hariot. Nu-Iron
Fresh Codiumfragile(sur.) Hariot., dry in the sun 2 days after cleaning, 60 DEG C of drying, take 500g to pulverize and cross 40 mesh sieves, add 10L water, adjusting pH is
12.0, it is warming up to 70 DEG C, stirring is extracted 2 hours, is repeated 3 times, obtains Codiumfragile(sur.) Hariot. Polyose extraction using 750 mesh filter cloth plate-and-frame filtration
Liquid.Add citric acid in filtrate, be warming up to 70 DEG C, adjust and keep pH 7.5-8.5, while stirring Deca 2mol/L trichlorine
Change ferrous solution, to producing reddish brown precipitation, maintain reaction temperature to be 70 DEG C and continue stirring reaction 3 hours.Reactant liquor is in 3500rpm
It is centrifuged 10 minutes under rotating speed, supernatant, by the NF membrane of molecular cut off 500Da, repeatedly filters and to concentrated solution electrical conductivity is
12us/cm, is spray-dried, obtains Codiumfragile(sur.) Hariot. Nu-Iron.
Embodiment 3：The impact to spinach growth for the green algae polysaccharide ferrum
It is green algae polysaccharide ferrum, NaFeEDTA sodium for examination fertilizer.3 process of test point（Polyferose group（By embodiment 1 preparation technology system
?）, NaFeEDTA sodium group, clear water matched group）, each processes 2 repetitions, and each repeats kind of 10 plants of Herba Spinaciaes, applying fertilizer dilute with water
After 1000 times, carry out foliar spray process every 7 days, after spraying 1 week, carry out each index determining, 1 week after spraying for the last time, will all
Herba Spinaciae harvests, and measures dry weight, and result is as shown in the table.
The impact to spinach growth for table 1 green algae polysaccharide
Under this experiment condition, foliar spray green algae polysaccharide ferrum, NaFeEDTA sodium all can increase in various degree Spinach Leaf number, leaf color value,
Vane thickness and Herba Spinaciae dry weight, wherein foliar spray iron fertilizer also have preferable performance on increasing vane thickness and increase Herba Spinaciae dry weight,
The advantage of green algae polysaccharide ferrum becomes apparent from.
Embodiment 4：Sea grass polysaccharide ferrum is applied to the experimentation of iron deficiency mice
Experimental subject：Iron deficiency mice
Using medicine：Sea grass polysaccharide ferrum（Prepared by embodiment 1）, red source reaches（Positive control drug）, ferrous sulfate
Experiment mice divides 6 groups, normal mouse matched group, iron deficiency control mice group, positive controls（Red source reaches）, ferrous sulfate
Group, sea grass polysaccharide ferrum group, carry out corresponding medicine gavage daily to mice（Normal mouse combination iron deficiency mice group is filled with equivalent clear water
Stomach）, gavage is after 2 weeks, hemoglobin situation of change in detection mouse blood.
Fig. 1 is applied to the impact to content of hemoglobin in the experiment of iron deficiency mice for sea grass polysaccharide ferrum.By experimental result
As can be seen that after sea grass polysaccharide ferrum was to iron deficiency mouse stomach two weeks, in mouse blood, content of hemoglobin is already close to normal water
Flat, and effect is suitable with positive controls.Inorganic molysite（Ferrous sulfate）The mice of gavage, content of hemoglobin is not significantly
Change, most of ferrous ion is oxidized, directly discharged by feces after precipitation（Detected by stool in mice）, and with bright
Aobvious constipation phenomenon.As can be seen here, inorganic molysite utilization rate in animal body is extremely low, and the side effect such as constipation occurs, green
Polysaccharides ferrum, as a kind of organic complex ferrum, can effectively improve the content of hemoglobin of iron deficiency mice, with treatment iron-deficient
The medicine of anemia（Red source reaches）Action effect is suitable.
Embodiment 5：Green algae polysaccharide ferrum is applied to sow and its piglet
Experimental subject：Term close in-pig 24, is randomly divided into 3 groups
Using medicine：Codiumfragile(sur.) Hariot. polyferose（Prepared by embodiment 2）, domestic animal sanguinin（Positive control drug）
3 groups of experiment point, control group fed basal diet, positive controls feed basal diet+injection domestic animal sanguinin, green algae polysaccharide ferrum
Group feeding basal diet+Codiumfragile(sur.) Hariot. polyferose 1kg/t.
The impact to number born of sow and piglet survival ratio, body weight for the table 2 green algae polysaccharide ferrum
| Group | Nest litter size | Survival rate | Nest produces son's counterpoise of living |
| Matched group | 10±1.22 | 85.92 | 1.21±0.05 |
| Positive controls | 11±0.78 | 95.75 | 1.27±0.07 |
| Green algae polysaccharide ferrum group | 11±0.41 | 95.43 | 1.39±0.06 |
Table 2 is the impact to number born of sow and piglet survival ratio, body weight for the green algae polysaccharide ferrum.As can be seen that green algae polysaccharide ferrum
Absorbance is high, after feeding in-pig, makes more Organic Irons pass to fetus by placental barrier, thus body during piglet birth
Interior iron promoter is more, so that immunoglobulin concentrations in blood is improved, thus improving the connatae vitality of piglet and health status.
The nest litter size of green algae polysaccharide ferrum group increased, and survival rate is substantially improved, and effect is suitable with injection domestic animal sanguinin；Chlorella simultaneously
Polysaccharide iron prop lives son's counterpoise lifting substantially, higher by 14.9% than matched group, higher by 9.45% than the positive controls of injection domestic animal sanguinin.
Claims (5)
1. a kind of green algae polysaccharide iron complexes are it is characterised in that it is to be prepared into green algae polysaccharide and high price ferrum for synthesis material
Arrive.
2. a kind of method preparing green algae polysaccharide iron complexes described in claim 1 is it is characterised in that it comprises the following steps：
1）Green algae polysaccharide is extracted：After chlorella drying and crushing, add the water of 10-20 times of volume, tune pH is 9.0-12.0,60-80 DEG C
2-4 hour is extracted in lower stirring, and continuous 2-3 rear extracting solution of extraction merges, and obtains green algae polysaccharide using 750 mesh filter-cloth filterings and extracts
Liquid；
2）The preparation of green algae polysaccharide iron complexes：The pH being added thereto to citric acid regulation green algae polysaccharide extracting solution is 6.5-
9.0, solution is warming up to 70-90 DEG C, is slowly added dropwise iron chloride or the ferrum sulfuricum oxydatum solutum of 2mol/L thereto, until producing reddish brown
Color precipitates, and continues stirring reaction 2-6 hour at 70-90 DEG C, and reactant liquor is centrifuged 10-20 minute, supernatant under 3500rpm rotating speed
Liquid is crossed NF membrane and is obtained nanofiltration concentrate, and nanofiltration concentrate is spray-dried, and obtains final product green algae polysaccharide iron complexes.
3. the preparation method of green algae polysaccharide iron complexes as claimed in claim 2 is it is characterised in that the retention of described NF membrane
Molecular weight is 300-500Da.
4. the preparation method of green algae polysaccharide iron complexes as claimed in claim 2 is it is characterised in that described supernatant can be many
Secondary NF membrane excessively, until concentrated solution electrical conductivity ＜ 30us/cm.
5. application in preparing feed additive or bio-feritlizer for the green algae polysaccharide iron complexes described in claim 1.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110194809A (en) * | 2019-06-18 | 2019-09-03 | 武汉轻工大学 | The preparation method and application of Codonopsis pilosula polysaccharide iron complexes |
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| CN1148600A (en) * | 1996-03-05 | 1997-04-30 | 中国科学院南海海洋研究所 | Seaweed polysaccharide trace-element complex and its preparation method and usage |
| CN102351958A (en) * | 2011-08-22 | 2012-02-15 | 河南中医学院 | Preparation method and application of astragalus polysaccharide-calcium complex |
| CN103864950A (en) * | 2014-03-21 | 2014-06-18 | 张忠山 | Preparation method and application of low-molecular porphyra haitanensis polyferose compound |
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2016
- 2016-11-26 CN CN201611056612.8A patent/CN106478837B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1148600A (en) * | 1996-03-05 | 1997-04-30 | 中国科学院南海海洋研究所 | Seaweed polysaccharide trace-element complex and its preparation method and usage |
| CN102351958A (en) * | 2011-08-22 | 2012-02-15 | 河南中医学院 | Preparation method and application of astragalus polysaccharide-calcium complex |
| CN103864950A (en) * | 2014-03-21 | 2014-06-18 | 张忠山 | Preparation method and application of low-molecular porphyra haitanensis polyferose compound |
Non-Patent Citations (1)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110194809A (en) * | 2019-06-18 | 2019-09-03 | 武汉轻工大学 | The preparation method and application of Codonopsis pilosula polysaccharide iron complexes |
| CN110194809B (en) * | 2019-06-18 | 2021-10-26 | 武汉轻工大学 | Preparation method and application of codonopsis pilosula polysaccharide-iron compound |
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