CN106459874A - 采用动态阈值的流式细胞仪信号峰的鉴定 - Google Patents
采用动态阈值的流式细胞仪信号峰的鉴定 Download PDFInfo
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- CN106459874A CN106459874A CN201580033797.8A CN201580033797A CN106459874A CN 106459874 A CN106459874 A CN 106459874A CN 201580033797 A CN201580033797 A CN 201580033797A CN 106459874 A CN106459874 A CN 106459874A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N2015/1461—Coincidence detecting; Circuits therefor
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Application Number | Priority Date | Filing Date | Title |
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US201462008345P | 2014-06-05 | 2014-06-05 | |
US62/008,345 | 2014-06-05 | ||
PCT/US2015/033907 WO2015187783A1 (en) | 2014-06-05 | 2015-06-03 | Flow cytometer signal peak identification employing dynamic thresholding |
Publications (2)
Publication Number | Publication Date |
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CN106459874A true CN106459874A (zh) | 2017-02-22 |
CN106459874B CN106459874B (zh) | 2020-06-30 |
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CN201580033797.8A Active CN106459874B (zh) | 2014-06-05 | 2015-06-03 | 采用动态阈值的流式细胞仪信号峰的鉴定 |
Country Status (4)
Country | Link |
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US (3) | US9903803B2 (zh) |
EP (1) | EP3152294B1 (zh) |
CN (1) | CN106459874B (zh) |
WO (1) | WO2015187783A1 (zh) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109540806A (zh) * | 2018-10-16 | 2019-03-29 | 华中科技大学 | 一种利用动态阈值重心法提取共焦显微峰值的方法 |
CN109946218A (zh) * | 2019-04-01 | 2019-06-28 | 北京工业大学 | 一种利用sybr green i单染色技术检测污水处理活性污泥中聚糖菌的方法 |
CN111239010A (zh) * | 2018-11-29 | 2020-06-05 | 罗伯特·博世有限公司 | 用于检测颗粒的方法和装置 |
CN112683873A (zh) * | 2021-03-22 | 2021-04-20 | 泛肽生物科技(浙江)有限公司 | 液态芯片的检测模型构建方法及装置、分析方法及装置 |
CN113167716A (zh) * | 2019-01-21 | 2021-07-23 | 以埃森生物科学股份有限公司的名义经营的埃森仪器股份有限公司 | 具有用于流式细胞术研究的流体样本的优化稀释的数据分析的流式细胞术 |
Families Citing this family (8)
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EP3152545A2 (en) * | 2014-06-05 | 2017-04-12 | IntelliCyt Corporation | Flow cytometer with optical system assembly |
CN107615065B (zh) | 2015-03-23 | 2020-07-24 | 以埃森生物科学股份有限公司的名义经营的埃森仪器股份有限公司 | 对生物材料评估腺病毒或腺相关病毒的病毒类型的非缔合的病毒尺寸颗粒 |
JP6683362B2 (ja) * | 2016-04-19 | 2020-04-22 | メタウォーター株式会社 | 試料中の微粒子の数を推定する方法 |
US11520775B2 (en) * | 2020-01-24 | 2022-12-06 | Sartorius Bioanalytical Instruments, Inc. | Adaptive data sub-sampling and computation |
US11709116B2 (en) | 2020-02-04 | 2023-07-25 | Sartorius Bioanalytical Instruments, Inc. | Liquid flourescent dye concentrate for flow cytometry evaluation of virus-size particles and related products and methods |
CN115176142A (zh) * | 2020-02-27 | 2022-10-11 | 贝克顿·迪金森公司 | 用于在细胞分选中标识饱和数据信号的方法及用于其的系统 |
WO2023163813A1 (en) * | 2022-02-24 | 2023-08-31 | Becton, Dickinson And Company | Methods and systems for evaluating flow cytometer data for the presence of a coincident event |
KR20230138571A (ko) | 2022-03-23 | 2023-10-05 | 삼성전자주식회사 | 바이오 입자 검출 장치 및 방법 |
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US20060259253A1 (en) * | 1999-10-21 | 2006-11-16 | Dako Colorado, Inc. | Systems for transiently dynamic flow cytometer analysis |
US20100284016A1 (en) * | 2009-05-06 | 2010-11-11 | The Regents Of The University Of California | Optical cytometry |
US20110089328A1 (en) * | 2009-10-20 | 2011-04-21 | Diagnostic Chips, LLC | Electrokinetic microfluidic flow cytometer apparatuses with differential resistive particle counting and optical sorting |
CN102830053A (zh) * | 2003-03-28 | 2012-12-19 | 英格朗公司 | 用于分拣颗粒和提供性别分拣的动物精子的设备、方法和程序 |
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US3758058A (en) | 1971-09-27 | 1973-09-11 | Amana Refrigeration Inc | Shipping mount for room air conditioners |
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EP0822404B1 (en) | 1996-07-30 | 2009-06-17 | Siemens Healthcare Diagnostics Inc. | Optical system for a hematology analytical instrument |
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JP5904996B2 (ja) * | 2011-03-29 | 2016-04-20 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析装置、光分析方法並びに光分析用コンピュータプログラム |
JP6396911B2 (ja) * | 2012-10-15 | 2018-09-26 | ナノセレクト バイオメディカル, インコーポレイテッド | 粒子を選別するためのシステム、装置、および、方法 |
CA2796489C (en) | 2012-11-22 | 2019-08-27 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence | Man-portable device for detecting hazardous material |
EP3013947B1 (en) | 2013-06-28 | 2018-10-24 | IntelliCyt Corporation | Processing biological material for flow cytometry evaluation for virus particles |
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- 2015-06-03 EP EP15803911.5A patent/EP3152294B1/en active Active
- 2015-06-03 US US15/316,427 patent/US9903803B2/en active Active
- 2015-06-03 CN CN201580033797.8A patent/CN106459874B/zh active Active
-
2018
- 2018-01-15 US US15/871,731 patent/US10184878B2/en active Active
- 2018-11-30 US US16/206,350 patent/US10520420B2/en active Active
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US20060259253A1 (en) * | 1999-10-21 | 2006-11-16 | Dako Colorado, Inc. | Systems for transiently dynamic flow cytometer analysis |
CN102830053A (zh) * | 2003-03-28 | 2012-12-19 | 英格朗公司 | 用于分拣颗粒和提供性别分拣的动物精子的设备、方法和程序 |
US20100284016A1 (en) * | 2009-05-06 | 2010-11-11 | The Regents Of The University Of California | Optical cytometry |
US20110089328A1 (en) * | 2009-10-20 | 2011-04-21 | Diagnostic Chips, LLC | Electrokinetic microfluidic flow cytometer apparatuses with differential resistive particle counting and optical sorting |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109540806A (zh) * | 2018-10-16 | 2019-03-29 | 华中科技大学 | 一种利用动态阈值重心法提取共焦显微峰值的方法 |
CN109540806B (zh) * | 2018-10-16 | 2019-11-05 | 华中科技大学 | 一种利用动态阈值重心法提取共焦显微峰值的方法 |
CN111239010A (zh) * | 2018-11-29 | 2020-06-05 | 罗伯特·博世有限公司 | 用于检测颗粒的方法和装置 |
CN113167716A (zh) * | 2019-01-21 | 2021-07-23 | 以埃森生物科学股份有限公司的名义经营的埃森仪器股份有限公司 | 具有用于流式细胞术研究的流体样本的优化稀释的数据分析的流式细胞术 |
CN109946218A (zh) * | 2019-04-01 | 2019-06-28 | 北京工业大学 | 一种利用sybr green i单染色技术检测污水处理活性污泥中聚糖菌的方法 |
CN109946218B (zh) * | 2019-04-01 | 2021-10-15 | 北京工业大学 | 一种利用sybr green i单染色技术检测污水处理活性污泥中聚糖菌的方法 |
CN112683873A (zh) * | 2021-03-22 | 2021-04-20 | 泛肽生物科技(浙江)有限公司 | 液态芯片的检测模型构建方法及装置、分析方法及装置 |
CN112683873B (zh) * | 2021-03-22 | 2021-06-01 | 泛肽生物科技(浙江)有限公司 | 液态芯片的检测模型构建方法及装置、分析方法及装置 |
Also Published As
Publication number | Publication date |
---|---|
US10520420B2 (en) | 2019-12-31 |
EP3152294A1 (en) | 2017-04-12 |
US20170205331A1 (en) | 2017-07-20 |
US10184878B2 (en) | 2019-01-22 |
US20180136108A1 (en) | 2018-05-17 |
US9903803B2 (en) | 2018-02-27 |
CN106459874B (zh) | 2020-06-30 |
EP3152294B1 (en) | 2021-03-10 |
US20190094122A1 (en) | 2019-03-28 |
WO2015187783A1 (en) | 2015-12-10 |
EP3152294A4 (en) | 2017-06-28 |
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