CN106420596B - A kind of composition of the extract containing corn kernel and its application - Google Patents
A kind of composition of the extract containing corn kernel and its application Download PDFInfo
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- CN106420596B CN106420596B CN201611081729.1A CN201611081729A CN106420596B CN 106420596 B CN106420596 B CN 106420596B CN 201611081729 A CN201611081729 A CN 201611081729A CN 106420596 B CN106420596 B CN 106420596B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/27—Zinc; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Abstract
The invention discloses a kind of composition of extract containing corn kernel and its applications, it is intended to provide a kind of attachment that can effectively reduce pollutant, reinforce the natural barrier function and anti-irritant ability of skin, remove free radical, strengthen Antioxidative Defense System, the composition for the problems such as keeping the skin wet simultaneously to skin and energy, effectively resisting skin allergy caused by environmental contaminants, aging；Technical points mainly include following components in parts by weight: 8-30 parts of corn kernel extract, 5-25 parts of Moringa seed extract of wing seed, 5-15 parts of smithsonite extract, 5-10 parts of Chondrus crispus extract, 5-15 parts of boldo tree leaf extract；The invention further relates to using the composition as the application of antipollution skin-protection product additive；Belong to cosmetic technical field.
Description
Technical field
The present invention relates to a kind of compositions, are a kind of compositions of extract containing corn kernel specifically, the present invention also relates to
And using the composition as the application of antipollution skin-protection product additive, belong to cosmetic technical field.
Background technique
As production and economic express delivery increase, the continuous deterioration of environment, air pollution is constantly aggravated, the pollution of the mankind
Source is constantly increasing, such as the pollution sources that dust, haze, sandstorm, radiation, PM2.5 are physical；As secondhand smoke, vehicle exhaust,
The pollution sources chemically such as industrial pollution, exhaust gas.It is easy to cause following skin problem: 1. leading to skin allergy: is a large amount of in air
The floating material allergy of disperse causes skin pore blocking and allergic symptom, makes one to feel itch, plays erythema wheal, or even triggering
Nettle rash and eczema.In addition, physical pollution sources cause the particle in air excessive, degree of light transmission is reduced, and makes to reach ground
Neighbouring ultraviolet light is weakened.Various viruses in near-earth air are swum in this way, and bacterium and fungi become to be not easy by ultraviolet light
It kills, it is poor for constitution, there is a skin allergy, for the people of skin injury, this quasi-microorganism is reached with the dust particles of air
To skin, and it is adhered to skin surface, easily causes the infection of skin, such as epifolliculitis, sweat stain, herpe simplex etc. has fash
People can aggravate.2. leading to the aging of skin: pollutants in air, such as haze, vehicle exhaust can make the pole of skin generation excess
Unstable free radical, these free radicals are by damaging the DNA of cell membrane and human body own health, thus accelerate organism aging process,
Aging including skin, if skin is obscure, pigmentation, skin is easy relaxation, microgroove or wrinkle occurs, has dry skin, even
Lead to skin canceration.
Cosmetics on the market are by film forming component or to add ultraviolet light attractant, physical interleaving agent mostly at present,
The pollution of air-isolation and support anti-ultraviolet injury, effect is relatively simple, cannot comprehensively resist various pollution sources, antipollution
The effect of it is not significant.
Summary of the invention
In view of the above-mentioned problems, the first object of the present invention is to provide a kind of adhesion amount that can effectively reduce environmental contaminants,
The natural barrier function and anti-irritant ability of skin are reinforced in murder by poisoning to preventing from heavy metal, remove free radical, strengthen antioxygen chemoprevention
Imperial system, while the problems such as keep the skin wet to skin and energy, effectively resist skin allergy caused by environmental contaminants, aging
Composition.
The second object of the present invention is to provide application of this composition as antipollution skin-protection product additive.
To solve first technical problem, first technical solution provided by the invention is such that
A kind of composition of the extract containing corn kernel, is mainly grouped as by the group of following parts by weight: corn kernel extract 8-
30 parts, 5-25 parts of Moringa seed extract of wing seed, 5-15 parts of smithsonite extract, 5-10 parts of Chondrus crispus extract, Bordeaux
5-15 parts of leaf extract.
Further, the composition of above-mentioned a kind of extract containing corn kernel further includes 2-10 parts of Withania kansuensis root extract.
Further, the composition of above-mentioned a kind of extract containing corn kernel further includes 5-20 parts of olea europaea fruit water.
Further, the composition of above-mentioned a kind of extract containing corn kernel further includes hydrolysis Candida extract 1-6
Part.
Further, the composition of above-mentioned a kind of extract containing corn kernel, is mainly grouped as by the group of following parts by weight:
10-25 parts of corn kernel extract, 10-20 parts of Moringa seed extract of wing seed, 8-12 parts of smithsonite extract, Chondrus crispus extracts
6-8 parts of object, 6-10 parts of boldo tree leaf extract, 4-8 parts of Withania kansuensis root extract, 10-16 parts of olea europaea fruit water, hydrolyze false silk ferment
Female extract 2-5 parts.
Further, in a kind of composition of extract containing corn kernel described above, the wherein system of corn kernel extract
It is standby that steps are as follows:
1) corn kernel is freeze-dried, crushes, obtains corn kernel coarse powder, in the ratio addition water of solid-liquid ratio 1:3-5, then plus
Enter cellulase, the additive amount of cellulase is 10U/g, digests 2-3h；
2) the corn kernel coarse powder handled by step 1) is taken, is added the 60-80%'s of corn kernel coarse powder 15-30 times of volume of weight
Ethanol water 70-80 DEG C refluxing extraction 1-3 times, extract 4-8h every time, combined extract is subsequently placed in centrifuge and carries out
Centrifugation, centrifuge speed 5000r/min, centrifugation time be 10-20min after obtain supernatant, by supernatant in 40-60 DEG C,
Revolving speed carries out rotary evaporation under the conditions of being 40-70r/min, is concentrated into the 1/3-1/4 of stoste volume, obtains concentrate；
3) after the concentrate for obtaining step 2) adds water dispersion, upper macroporous resin column, successively with the volume of 3-5 times of column volume
The ethanol water that score is 30-40% elutes, and the ethanol water that the volume fraction of 4-6 times of column volume is 50-60% elutes；
Merge eluent, is concentrated into the 1/4-1/6 of original weight amount, concentrate is placed in spray dryer and is spray-dried, obtain corn kernel and mention
Take object.
The last one technical solution provided by the invention is the composition of the extract containing corn kernel as antipollution skin care
The application of product additive.
Compared with prior art, combination provided by the invention has following technological merit:
1, the corn kernel extract in combination provided by the invention is by promoting the tired skin that synthesizes of ATP to inject new energy
Amount, while also having and resisting townie invasion to protect skin, have the function of antipollution, anti peroxidation of lipid, antibacterial；
Wing seed Moringa seed extract, cationic protein (6000-13000 dalton) and calcium, iron, potassium rich in vitamin, low molecular weight
Equal minerals, meanwhile, the active constituent of fabulous flocculating properties is contained in wing seed moringa seeds, seed water extract can be substantially reduced mixed
Bacterial content in the turbidity of water, hardness and mixed water has lotus-effect, reduces pollutant and adheres to skin surface, protects cell,
Murder by poisoning to preventing from heavy metal, plays a protective role to skin；Smithsonite extract is rich in zinc, is closed by inducing metal sulfoprotein
At playing the role of gene protection (protection DNA), improve the protective effect that cell is directed to UVB noci-influence, it is dirty to facilitate isolation
Heavy metal in dye has long-term protective to alleviation environmental pressure and to antipollution；Chondrus crispus extract, by thickening
Water adipose membrane reinforces the natural barrier of skin, also other active constituents can be helped preferably to enter skin, active function is allowed to obtain
More preferably, it equably plays；Further, it is also possible to supplement minerals, it contains all minerals and microelement in skin ingredient,
Such as calcium, zinc, phosphorus, magnesium, copper, iron, manganese can increase the rouge content in mature skin, and promote the metabolic and anti-ageing of cell
Always；Boldo tree leaf extract activates the synthesis of skin beta-alexin (a kind of Endogenous antimicrobial polypeptide), enhances the natural anti-of skin
Fungus strain system, reduces bacteria breed, purifies skin, inhibits inflammatory factor, erythema of dispelling, and careful pore improves oily skin.
Withania kansuensis root extract is rich in glucoside, can promote dermal cell growth under extreme condition and enhances the anti-of skin
Imperial function has the function of restoring skin function, promote cytothesis and rebuilding barrier function.
Olea europaea fruit water is rich in a variety of polyphenol, especially olive polyphenol, has free radical resisting, and anti-aging is anti-oxidant, increases
Add skin elasticity and other effects, can reduce because pollution, ultraviolet light etc. are injury caused by skin, have skin lightening effect, test proves it
There are also antibacterial, anti-inflammatory, anti-irritant effects.
Candida extract is hydrolyzed, autophagy system can be enhanced, stimulate the formation of autophagosome, autophagosome is responsible for dissociation
Cell component, and they are delivered in lysosome and is decomposed, the toxin expelling process of cell can be promoted, reduce oxidized protein and mistake
The content of lipid oxide；Cheek wrinkle is smoothed, the colour of skin of " toxic " skin is improved.
2, the present composition is collocated with each other by both wing seed Moringa seed extract and smithsonite extract, to reduce
Pollutant is attached to skin surface, and fights injury of the heavy metal to skin in pollutant；Chondrus crispus extract and Withania kansuensis
The collocation of both root extracts, reinforces the natural barrier function and defense function of skin, effectively prevent water loss, while being isolated outer
The pollutant on boundary；Corn kernel extract, boldo tree leaf extract, olea europaea fruit water can enhance the natural antibacterial system of skin
System reduces bacteria breed, purifies skin, inhibits inflammatory factor, anti-irritation of the enhancing skin to environmental contaminants.In addition, with
Upper plant extracts and hydrolysis Candida extract can all keep the skin wet and energy for skin, the group of Multiple components interworking composition
Closing object also has effects that efficient free radical resisting and oxidation resistant, further skin is helped to reduce because pollution, ultraviolet light etc. are to skin
It is injured caused by skin.
3, composition of the invention effectively reduces the adhesion amount of environmental contaminants, a confrontation huge sum of money by the above number of ways
The natural barrier function and anti-irritant ability of skin are reinforced in the murder by poisoning of category, remove free radical, strengthen Antioxidative Defense System,
The problems such as keeping the skin wet and energy to skin simultaneously, effectively resisting skin allergy caused by environmental contaminants, aging, plays anti-pollution
The effect of dye.
Specific embodiment
With reference to embodiment, claim of the invention is described in further detail, but do not constituted pair
Any restrictions of the invention, the modification of anyone limited times made in the claims in the present invention protection scope etc, still exist
Within claims of the invention.
Wing seed Moringa seed extract is purchased from BASF China, and smithsonite extract is biochemical purchased from Shanghai Pu grace
Science and Technology Ltd., Chondrus crispus extract are purchased from hundred Hao Bo Co., Ltd of Guangzhou, and Withania kansuensis root extract hydrolyzes false silk ferment
Female extract is purchased from Guangzhou Bi Sheng trade Co., Ltd, and olea europaea fruit water is purchased from Guangzhou Te Nusi Chemical Co., Ltd., bohr
More leaf extracts are purchased from Guangzhou Xian Ting trade Co., Ltd.
Embodiment 1
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract 8g,
The wing seed Moringa seed extract 25g, smithsonite extract 5g, Chondrus crispus extract 10g, boldo tree leaf extract 5g.
Embodiment 2
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
30g, the wing seed Moringa seed extract 5g, smithsonite extract 15g, Chondrus crispus extract 5g, boldo tree leaf extract
15g。
Embodiment 3
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
20g, the wing seed Moringa seed extract 15g, smithsonite extract 10g, Chondrus crispus extract 8g, boldo tree leaf extract
10g。
Embodiment 4
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
10g, the wing seed Moringa seed extract 20g, smithsonite extract 8g, Chondrus crispus extract 9g, boldo tree leaf extract 8g,
Withania kansuensis root extract 6g.
Embodiment 5
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
25g, the wing seed Moringa seed extract 10g, smithsonite extract 13g, Chondrus crispus extract 6g, boldo tree leaf extract
12g, olea europaea fruit water 12g.
Embodiment 6
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
12g, the wing seed Moringa seed extract 18g, smithsonite extract 6g, Chondrus crispus extract 8g, boldo tree leaf extract 6g,
Hydrolyze Candida extract 3g.
Embodiment 7
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
28g, the wing seed Moringa seed extract 7g, smithsonite extract 14g, Chondrus crispus extract 7g, boldo tree leaf extract
13g, Withania kansuensis root extract 10g, olea europaea fruit water 5g.
Embodiment 8
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
15g, the wing seed Moringa seed extract 22g, smithsonite extract 9g, Chondrus crispus extract 9g, boldo tree leaf extract 7g,
Withania kansuensis root extract 2g hydrolyzes Candida extract 6g.
Embodiment 9
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
20g, the wing seed Moringa seed extract 15g, smithsonite extract 10g, Chondrus crispus extract 8g, boldo tree leaf extract
10g, olea europaea fruit water 20g hydrolyze Candida extract 1g.
Embodiment 10
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
25g, the wing seed Moringa seed extract 10g, smithsonite extract 12g, Chondrus crispus extract 6g, boldo tree leaf extract
10g, Withania kansuensis root extract 4g, olea europaea fruit water 16g hydrolyze Candida extract 2g.
Embodiment 11
A kind of composition of extract containing corn kernel provided by the invention, is grouped as by following groups: corn kernel extract
10g, the wing seed Moringa seed extract 20g, smithsonite extract 8g, Chondrus crispus extract 8g, boldo tree leaf extract 6g,
Withania kansuensis root extract 8g, olea europaea fruit water 10g hydrolyze Candida extract 5g.
The composition of the extract containing corn kernel in embodiment 1 to 11, the corn kernel extract the preparation method is as follows:
1) corn kernel is freeze-dried, crushes, obtains corn kernel coarse powder, add in the ratio of solid-liquid ratio 1:3-5 (preferably 1:4)
Enter water, add cellulase, the additive amount of cellulase is 10U/g, is digested 2-3h (preferably 2.5h)；
2) the corn kernel coarse powder handled by step 1) is taken, corn kernel coarse powder 15-30 times of (preferably 20 times) volume of weight is added
60-80% (preferably 70%) ethanol water in 70-80 DEG C of (preferably 75 DEG C) refluxing extraction 1-3 times (preferably 2 times), every time
It extracts 4-8h (preferably 6h), combined extract is subsequently placed in centrifuge and is centrifuged, centrifuge speed 5000r/min, from
The heart time be 10-20min (preferably 15min) after obtain supernatant, by supernatant in 40-60 DEG C of (preferably 50 DEG C), revolving speed 40-
Rotary evaporation is carried out under the conditions of 70r/min (preferably 50r/min), the 1/3-1/4 (preferably 1/3) of stoste volume is concentrated into, obtains
Concentrate；
3) after the concentrate for obtaining step 2) adds water dispersion, upper macroporous resin column, successively with 3-5 times of (preferably 4 times) column
The volume fraction of volume is that the ethanol water of 30-40% (preferably 35%) elutes, the volume of 4-6 times of (preferably 5 times) column volume
Score is that the ethanol water of 50-60% (preferably 60%) elutes；Merge eluent, is concentrated into the 1/4-1/6 of original weight amount (preferably
1/5), concentrate is placed in spray dryer and is spray-dried, corn kernel extract is obtained.
The preparation method of above-mentioned composition is to weigh each component according to any one mass number in embodiment 1 to 11, first
After corn kernel extract is dissolved with appropriate amount of deionized water, then it is uniformly mixed with other ingredients.
When specifically used, a kind of composition of the extract containing corn kernel prepared by the present invention is dissolved with appropriate amount of deionized water
After mixing evenly, it is added directly in skin care item matrix, stirs evenly, additive amount is the 0.5-10wt% of skin care item gross mass.
A kind of shield of extract containing corn kernel provided by the invention is given below in order to better illustrate the present invention the advantages of
The effect of skin composition, tests:
One, about the comparative example of efficacy test
Comparative example 1
Wherein corn kernel extract is the product of purchase, remaining is same as in Example 10.
Comparative example 2
Composition only contains Borago officinalis Borage extract 6g, olea europaea fruit water 16g, hydrolyzes Candida extract 2g.
Comparative example 3
Only contain cream matrix.
Comparative example 4
The a certain commercially available antipollution frost of purchase.
Two, adsorbing powder dust is tested
1, laboratory sample: the composition of Example 3-10 preparation is added in cream matrix, additive amount is 5%；
2, test method: 45 roads of selection assist diligent personnel, are divided into 9 groups, and both hands are cleaned with hand cleanser and done by every group of 5 people
Only, it is dried with paper handkerchief.Every group of personnel smear the cream matrix of comparative example 3 in left forearm on about 2 square centimeters of area
0.2g is smeared respectively on about 2 square centimeters of area in right forearm and is added 5% embodiment 3-10, the commercially available of comparative example 4 resists
Pollute frost 0.2g.Allow they on road side 4 hours on duty with 3M adhesive tape by the dust on forearm it is viscous under, weighing is averaged
Value.Test result is shown in Table 1.
1 adsorbing powder dust test result of table
Above-mentioned test data show right forearm smear addition composition of the embodiment of the present invention cream after, right forearm powder
Dust quantity reduction reaches 55% or more, hence it is evident that higher than the cream matrix of comparative example 3 and the Dust Capacity slip of commercially available antipollution frost, says
Bright composition of the invention can significantly reduce the absorption of dust on the skin.
Three, barrier function is tested
Normal skin has very strong barrier function, and extraneous harmful substance can be prevented to enter skin.When barrier function damage,
Access is just formed between horn cell, moisture scatters and disappears from access.Therefore, when skin barrier less effective, moisture is percutaneously lost
Increase, otherwise reduces.Protective layer of skin is better, and water content is higher, and the numerical value that skin moisture is lost TEWL is lower.This survey
The power for trying out percutaneous moisture loss (TEWL) evaluation skin barrier function, to evaluate the antipollution function of the present composition
Effect.
Laboratory sample: the composition of Example 3-10 and comparative example 1-2 preparation is added in cream matrix, adds
Amount is 5%；And comparative example 3 cream matrix and comparative example 4 commercially available antipollution cream.
Test method: 96 female subjects of selection face skin more dry water shortage the age 20-45 years old, are divided into 11
Group, every group of 8 people detect first three days, and any cosmetics are not used in face, the facial TEWL value before measuring the 1st day unused product
(when detection, being popped one's head in the TEWL of CK MPA580, detection is tested 5 times by inspection skin, reads average value on computers).Every group by
Examination person will take equivalent cream to be applied on skin of face respectively, wherein left face smears the cream matrix of comparative example 3, right face smears remaining
Above-mentioned laboratory sample, it is sooner or later primary, it is used continuously 28 days, the 29th, 30 day deactivated product, the 31st day measurement TEWL.It the results are shown in Table
2。
2 barrier function test result of table
After above-mentioned test data shows the cream using addition composition of the embodiment of the present invention, skin moisture is lost
TEWL value illustrates that composition of the invention has enhancing lower than the cream matrix and the commercially available antipollution frost of comparative example 4 of comparative example 3
The effect of skin barrier function.
After cream using addition 10 composition of the embodiment of the present invention, TEWL value is lower than addition embodiment 3 and comparative example 2
The cream of composition illustrates to extract in corn kernel extract, wing seed Moringa seed extract, smithsonite extract, Chondrus crispus
On the basis of object, the collaboration of boldo tree leaf extract, with Withania kansuensis root extract, olea europaea fruit water and hydrolysis Candida extract
Collaboration, can effectively increase skin barrier function, reduce scattering and disappearing for moisture of skin.
After cream using comparative example 1, TEWL value is also relatively higher than the cream prepared using 10 composition of embodiment, explanation
The corn kernel extract prepared in this composition can effectively supplement skin moisture, reduce moisture loss, preferably increase skin
Barrier function.
Four, free radical effect assessment is removed
1, the preparation of sample
Composition prepared by the embodiment 3-10 composition prepared and comparative example 2 first after mixing, takes mixed in equal amounts
Object is made into the sample solution that mass fraction is 1% again, carries out experiment below.
2, ultra-oxygen anion free radical Scavenging activity is evaluated
The Tris-HCl buffer 4.5mL for taking 0.05mol/L pH=8.2, preheats 20min in 25 DEG C of water-baths.Again plus
Enter 1mL sample and the pyrogallol solution of 0.4mL 25mmol/L, after mixing, 5min is reacted in 25 DEG C of water-baths, 8mol/ is added
The HCl 1.0mL of L terminates reaction.Make reference with Tris-HCl buffer, absorbance value is surveyed at 299nm.Blank control 1mL
The solvent of sample substitutes sample.Clearance rate (D) is calculated according to formula (2).
Ultra-oxygen anion free radical clearance rate (%)=× 100% (2) [1- (A2/A1)]
A1 is the absorbance value of blank control in formula；A2 is the absorbance value of drug.
3, the Scavenging activity of hydroxy radical is evaluated
2mmol/L FeSO is sequentially added in 25mL colorimetric cylinder43mL, 1mmol/L H2O23mL shakes up, and is subsequently added into
6mmol/L salicylic acid 3mL, shakes up, and takes out after 37 DEG C of heating water bath 15min, surveys its absorbance；It is separately added into certain density
Prepare liquid shakes up, and continues heating water bath 15min, takes out and surveys its absorbance.Following formula is prepare liquid to the clear of hydroxy radical (OH)
Except rate:
Scavenging action to hydroxyl free radical (%)=[A1-A2- (A1-A3)]/A1 × 100% (3)
A1 is the absorbance value of reaction system before non-dosing product in formula；A2 is the absorbance that drug removes system after OH
Value；A3 is the absorbance value that system after OH is removed in blank control.
4, experimental result
Table 3
| Laboratory sample | Ultra-oxygen anion free radical clearance rate/% | Scavenging action to hydroxyl free radical/% |
| Embodiment 3 | 72.3 | 70.9 |
| Embodiment 4 | 76.2 | 74.8 |
| Embodiment 5 | 78.9 | 76.3 |
| Embodiment 6 | 80.2 | 78.2 |
| Embodiment 7 | 83.9 | 80.6 |
| Embodiment 8 | 84.8 | 81.6 |
| Embodiment 9 | 86.2 | 83.5 |
| Embodiment 10 | 90.6 | 88.9 |
| Comparative example 2 | 60.5 | 56.8 |
Above-mentioned test data shows the potent removing free radical of composition energy of the invention, and than 2 composition of comparative example
It is good to remove free radical effect, illustrates in corn kernel extract, wing seed Moringa seed extract, smithsonite extract, Chondrus crispus
Extract, boldo tree leaf extract collaboration on the basis of, then with Withania kansuensis root extract, olea europaea fruit water and hydrolysis Candida
After extract compounding, synergistic effect is played, improves the left and right of composition removing free radical, increases oxidation resistance.
Five, to propionibacterium acnes rejection ability
1, the preparation of sample
Composition prepared by embodiment 1-11 first after mixing, takes equal amount of mixture to be made into sample aqueous solution, carries out
It tests below.
2, experimental method
It respectively takes sample aqueous solution 1mL to be added separately in sterile glass test tube, topples over the previously prepared improvement GAM of 8mL respectively
Broth bouillon.Taking-up has divided pure and in 35 DEG C of constant temperature CO2The clinical propionibacterium acnes of incubator culture 48h, take 1mL respectively
Bacteria suspension injection has added in the human body culture medium test tube of solution of extract, does feminine gender with a teat glass without bacteria suspension
Control.Every kind of extract culture medium is successively inoculated with propionibacterium acnes since low concentration, is placed in 35 DEG C of perseverances after inoculation as early as possible
Warm CO2Incubator culture 48h.After culture, the liquid first extracted in human body culture medium carries out preliminary identification experiment, observes acne third
The Survival of acidfast bacilli.
3, experimental result:
The result shows that loseing has propionibacterium acnes raw in the culture medium of addition 1-11 of embodiment of the present invention composition
It is long, illustrate that the present composition has obvious inhibitory effect to propionibacterium acnes.
Six, stimulant tolerance is tested
Situations such as stimulation of the skin by xenobiotic pollutants can often cause red and swollen, itch, play erythema wheal, over time
Cutaneous autoimmune function can be reduced and form sensibility skin, seriously damage skin health, so repairing impaired and enhancing skin
Immunity is to resist external source to pollute the most fundamental mode, and antipollution effect of composition is tested by skin irritatin efficacy assessment.
Experimental method: using 3%SLS solution and 5% tartaric acid as stimulus, left and right forearm flexor skin is stimulated respectively
Until it is red and swollen, the cream of the composition containing 5% embodiment of the present invention 1 is then smeared in left front arm region, is applied in right forearm region
The cream for smearing the composition containing 5% embodiment of the present invention 10, test observation red swelling of the skin recession degree after 2 days.
Test result: 3%SLS stimulates left front arm region 1, and skin is obviously red and swollen, smears and contains 1 group of 5% embodiment of the present invention
Behind cream 2 days for closing object, the red and swollen of left front arm region 1 obviously subsides；After 5% tartaric acid stimulates right forearm region 2, skin is bright
Aobvious redness, after smearing cream 2 days of 10 composition Han 5% embodiment of the present invention, the swollen also obvious recession in right forearm region 2.
Illustrate that composition of the invention has the anti-red and swollen effect of apparent anti-irritant.
In conclusion the present invention passes through corn kernel extract, wing seed Moringa seed extract, smithsonite extract, ripple angle
Pitch dish extract, Withania kansuensis root extract, boldo tree leaf extract, hydrolysis Candida extract and a variety of days of olea europaea fruit water
Right active matter synergistic effect, effectively reduces the attachment of pollutant；Reinforce the natural barrier function of skin；Antibacterial anti-inflammatory increases flesh
Anti-irritation of the skin to environmental contaminants；Free radical is removed, strengthens Antioxidative Defense System, effectively resists environmental contaminants and cause
Skin allergy, aging the problems such as, play the role of resistant to pollution.
Claims (5)
1. a kind of composition of extract containing corn kernel, which is characterized in that the composition as antipollution skin-protection product additive,
It mainly includes following components in parts by weight: 8-30 parts of corn kernel extract, 5-25 parts of Moringa seed extract of wing seed, smithsonite mentions
Take 5-15 parts of object, 5-10 parts of Chondrus crispus extract, 5-15 parts of boldo tree leaf extract；
The corn kernel extract is made by following step:
1) corn kernel is freeze-dried, crushes, obtains corn kernel coarse powder, water is added in the ratio of solid-liquid ratio 1:3-5, adds fibre
Plain enzyme is tieed up, the additive amount of cellulase is 10U/g, digests 2-3h；
2) the corn kernel coarse powder handled by step 1) is taken, the ethyl alcohol of the 60-80% of corn kernel coarse powder 15-30 times of volume of weight is added
Aqueous solution, 70-80 DEG C refluxing extraction 1-3 times, extract 4-8h every time, combined extract, be subsequently placed in centrifuge carry out from
The heart, centrifuge speed 5000r/min, centrifugation time be 10-20min after obtain supernatant, by supernatant in 40-60 DEG C, turn
Speed carries out rotary evaporation under the conditions of being 40-70r/min, is concentrated into the 1/3-1/4 of stoste volume, obtains concentrate；
3) after the concentrate for obtaining step 2) adds water dispersion, upper macroporous resin column, successively with the volume fraction of 3-5 times of column volume
It is eluted for the ethanol water of 30-40%, the ethanol water that the volume fraction of 4-6 times of column volume is 50-60% elutes；Merge
Eluent is concentrated into the 1/4-1/6 of original weight amount, concentrate is placed in spray dryer and is spray-dried, and obtains corn kernel extract.
2. a kind of composition of extract containing corn kernel according to claim 1, which is characterized in that further include that Withania kansuensis root mentions
Take 2-10 parts of object.
3. a kind of composition of extract containing corn kernel according to claim 1, which is characterized in that further include olea europaea fruit
5-20 parts of water.
4. a kind of composition of extract containing corn kernel according to claim 1, which is characterized in that further include hydrolyzing false silk
1-6 parts of yeast extract.
5. a kind of composition of extract containing corn kernel according to claim 1, which is characterized in that the composition by
Following components in parts by weight is constituted: 10-25 parts of corn kernel extract, 10-20 parts of Moringa seed extract of wing seed, and smithsonite extract
8-12 parts, 6-8 parts of Chondrus crispus extract, 6-10 parts of boldo tree leaf extract, 4-8 parts of Withania kansuensis root extract, olive
10-16 parts of fruit water, hydrolyze 2-5 parts of Candida extract.
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| CN107550815A (en) * | 2017-09-18 | 2018-01-09 | 上海家化联合股份有限公司 | A kind of maize seed extract and its application in skin preparations for extenal use |
| CN108338965B (en) * | 2018-04-07 | 2020-09-11 | 广州市索柔生物科技有限公司 | Coarse grain composition and preparation method thereof |
| CN108888575A (en) * | 2018-08-09 | 2018-11-27 | 福建樱幼佳科技有限公司 | A kind of baby skin protection product and preparation method thereof |
| CN109157445B (en) * | 2018-09-07 | 2021-05-04 | 广东丸美生物技术股份有限公司 | Skin-care substrate with anti-pollution and anti-inflammatory effects and preparation method and application thereof |
| CN109276513A (en) * | 2018-10-12 | 2019-01-29 | 诺斯贝尔化妆品股份有限公司 | A kind of composite plant oil-control bilayer surfactant at Han You le bamboo powder end |
| EP4011361A4 (en) * | 2019-08-06 | 2023-08-16 | Natura Cosméticos S.A. | Topical cosmetic composition, use of the composition and tonic for facial application |
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| FR2949684B1 (en) * | 2009-09-04 | 2011-12-16 | Silab Sa | ACTIVE PRINCIPLE FROM CANDIDA SAITOANA AND COSMETIC USE FOR DETOXIFICATION OF SKIN CELLS |
| CN103976906B (en) * | 2014-05-29 | 2016-04-06 | 上海清轩生物科技有限公司 | A kind of plant extract liquid mixture with moisturizing antiinflammation |
| CN104490715A (en) * | 2015-01-08 | 2015-04-08 | 上海圣婕化妆品有限公司 | Anti-allergy stress relief face mask essence containing saccharide isomerate and preparation method thereof |
| CN105055261B (en) * | 2015-09-15 | 2018-09-11 | 广州智媛生物科技有限公司 | A kind of antipollution skin-protection product and preparation method thereof |
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