CN106405068A - Method for automatically adjusting displayed color during immunoassay - Google Patents
Method for automatically adjusting displayed color during immunoassay Download PDFInfo
- Publication number
- CN106405068A CN106405068A CN201610241016.0A CN201610241016A CN106405068A CN 106405068 A CN106405068 A CN 106405068A CN 201610241016 A CN201610241016 A CN 201610241016A CN 106405068 A CN106405068 A CN 106405068A
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- analysis instrument
- sample
- spectrophotometric analysis
- absorbance
- show color
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000003018 immunoassay Methods 0.000 title abstract 2
- 238000002835 absorbance Methods 0.000 claims abstract description 31
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 238000004364 calculation method Methods 0.000 claims abstract description 9
- 238000002798 spectrophotometry method Methods 0.000 claims description 33
- 238000005259 measurement Methods 0.000 claims description 17
- 230000003053 immunization Effects 0.000 claims description 14
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000013529 alpha-Fetoproteins Human genes 0.000 description 2
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000012113 quantitative test Methods 0.000 description 2
- 208000030507 AIDS Diseases 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 208000006379 syphilis Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a method for automatically adjusting a displayed color during immunoassay. The method is applied to immunodetection and employs a hardware unit used for sample detection, a host computer connected with the hardware unit and used for receiving determination data from the hardware unit and an output terminal used for displaying the calculation results of the host computer, wherein the hardware unit comprises an absorbance analyzer. The method comprises a step of detecting the absorbance of each sample by using the absorbance analyzer and a step of displaying the detected absorbance on the output terminal. The method can improve resolution and immunodetection efficiency.
Description
Technical field
The present invention relates to the result in a kind of immune detection shows, the method for adjust automatically Show Color more particularly, in immunizing dose.
Background technology
Present relatively advanced and universal immune detection is that hardware device measures data and passes on host computer, is then calculated and shows in outlet terminal.
But in existing immune detection, the color that outlet terminal result shows is fixing（Single）'s；Negative or positive findings are seemed to be not easy to differentiate.I.e.:The interface color of interface display result is just as, because there are 96 samples at an interface, each looks over, and takes energy very much, detects a lot of plates within one day, and each plate has 96 samples, and workload is very big.Just as detection AIDS, syphilis, doctor is concerned about positive sample very much, and 96 samples may only have 1 positive sample, if distinguished it is easy to see with color.If distinguished it is easy to miss without color.Because positive sample will go up report Disease Control and Prevention Center, do not allow to fail to report.
Content of the invention
The method that the main technical problem to be solved in the present invention is to provide adjust automatically Show Color in a kind of immunizing dose, it can improve distinguishing, improves immune detection efficiency.
For solving above-mentioned technical problem, the method that the present invention provides adjust automatically Show Color in a kind of immunizing dose, including：Carry out the hardware device of pattern detection, connect the host computer receiving hardware device measurement data, the outlet terminal of display host computer result of calculation, described hardware device includes spectrophotometric analysis instrument, and its method is：Detect the absorbance of each sample of sample by spectrophotometric analysis instrument, and correspondence shows in outlet terminal.（It is according to absorbance power adjust automatically that color shows.）
The host computer result of calculation that described outlet terminal shows is to carry out each sample reduction of sample and corresponding parameter shows.
The sample absorbance that described spectrophotometric analysis instrument records is bigger, and the sample Show Color of corresponding reduction is deeper, shows white when absorbance angle value is 0.
The structure of described spectrophotometric analysis instrument includes the light source of the standard light for producing irradiation sample, carries out the detection microscope group of leaded light towards sample and convert optical signals to electric signal and analyze the signal detection module of absorbance.
Described spectrophotometric analysis instrument circuit is coupled with described host computer.
Described spectrophotometric analysis instrument is the spectrophotometric analysis instrument measuring luminous intensity based on chemiluminescence.
Described spectrophotometric analysis instrument is the spectrophotometric analysis instrument surveying shade based on Gold standard.
Described spectrophotometric analysis instrument is the spectrophotometric analysis instrument based on fluorescence measurement fluorescence intensity.
The height of the result of the attainable immune response of the present invention can react and be shown as different colours on software.The color of display can be automatically adjusted by software according to measurement result.
The invention has the beneficial effects as follows：A kind of method of adjust automatically Show Color in immunizing dose, including the hardware device carrying out pattern detection, connect the host computer receiving hardware device measurement data, the outlet terminal of display host computer result of calculation, described hardware device includes spectrophotometric analysis instrument, and its method is：Detect the absorbance of each sample of sample by spectrophotometric analysis instrument, and correspondence shows in outlet terminal；Distinguishing can be improved, improve immune detection efficiency.
Brief description
Fig. 1 is an embodiment of the present invention qualitative detection sample schematic diagram；
Fig. 2 is an embodiment of the present invention quantitative determination sample schematic diagram；
Fig. 3 is an embodiment of the present invention.
Specific embodiment
Combine accompanying drawing below by specific embodiment the present invention is described in further detail.
The present invention is the result Display Technique applying in immune detection software.The a lot of result of immune detection is chromogenic reaction.Measurement result is related with color.Multisample is detected, for example：Enzyme mark detects, same plate can be placed 96 samples, it is low that each end value has height to have, and has feminine gender to have the positive it is meant that the color of each result is different from.If software shows 96 sample results of same color on same interface, people is not easily found yin and yang attribute result.
The present invention shows the color of result according to result adjust automatically.Color, with microwell plate solid colour, facilitates judged result.Negative to qualitative detection, positive, weak feminine gender, weakly positive can be easily discriminated.For quantitative determination, color and end value assume proportionate relationship, are also easy to distinguish Different Results on the software interface of outlet terminal.
In a kind of immunizing dose, the method for adjust automatically Show Color, includes as existing：Carry out the hardware device of pattern detection, connect the host computer receiving hardware device measurement data, the outlet terminal of display host computer result of calculation.Its distinctive points is, described hardware device includes spectrophotometric analysis instrument 5（As shown in Figure 3）, concrete grammar is：Detect the absorbance of each sample of sample by spectrophotometric analysis instrument 5 while other Data Detection, and correspondence shows in outlet terminal.
During enforcement, host computer result of calculation that described outlet terminal shows is to carry out each sample reduction of sample and corresponding parameter shows.
During enforcement, the sample absorbance that described spectrophotometric analysis instrument 5 records is bigger, and the sample Show Color of corresponding reduction is deeper, shows white when absorbance angle value is 0.
As shown in figure 3, the structure of spectrophotometric analysis instrument 5 includes the light source 1 of the standard light for producing irradiation sample, carries out the detection microscope group 3 of leaded light towards sample and convert optical signals to electric signal and analyze the signal detection module 4 of absorbance.Immediately below light source 1 side-lower, detection microscope group 3 be sample or sample 2 put position.The circuit of spectrophotometric analysis instrument 5 is coupled with described host computer.
During enforcement, described spectrophotometric analysis instrument 5 is to measure the spectrophotometric analysis instrument of luminous intensity based on chemiluminescence, based on the Gold standard survey spectrophotometric analysis instrument of shade or the spectrophotometric analysis instrument based on fluorescence measurement fluorescence intensity.
The present invention can adopt software algorithm technology, to result automatic colouring, the color with actual immune detection result close it is easy to find the positive, feminine gender, strong positive, weakly positive, by force negative, weak ' negative ' specimens.Assist operators efficiently watch result.
Refer to Fig. 1, qualitative detection example, corresponding sample color is deeper, and absorbance is bigger, and the positive of sample is also more obvious.
Fig. 1 is Display Realization interface（Can be to be shown on display terminal by software）.2 projects are wherein done：HbsAb（Hepatitis B surface antigen）, HbeAg（Hepatitis B virus e antigen）.Wherein PC is negative control, and PC is positive control, and QC is Quality Control, and OD value is absorbance.The absorbance of the corresponding sample of system detectio, enter row operation with absorbance thus draw the result of sample Lai.The higher color of absorbance is deeper, and absorbance angle value is to be white when 0.When not having ELIASA, it is the yin and yang attribute seeing shade and judged result by people's naked eyes.ELIASA is to enter, by absorbance, the yin and yang attribute that row operation is obtained a result.
A kind of linear relationship set up in terms of absorbance and color value by the software of the present invention.By the absorbance obtaining, calculate the value of color.The less color of absorbance is more shallow, and the bigger color of absorbance is deeper.This and the actual enzyme mark result color measuring are close.
Other immune detection also have such feature.Such as chemiluminescence measurement luminous intensity, Gold standard surveys shade, fluorescence measurement fluorescence intensity.This scheme can be adopted.
One key point of the present invention is that the height of the result of immune response can react and is shown as different colours on software.Software automatically adjusts the color of display according to measurement result, as long as measured value is higher, color is just deeper.
The all softwares doing immune detection at present do not have this to automatically adjust color function according to measured value.Best situation, the software of outlet terminal becomes color according to result of calculation if positive display is red.
The present invention is according to raw measurement data auto-changing color, makes Show Color close with actual color.Allow operator compare actual result and software results in measurement, easily pinpoint the problems.As in quantitative test, if standard items are put out of order, the color of standard items is not just arrangement from shallow to deep, and quantitative matched curve cannot matching.By color it is easy to see that problem.During observational measurement, distinguished by color and be also easily found the positive, feminine gender, strong positive, weakly positive, by force negative, weak ' negative ' specimens.It is an advantage of the invention that distinguish the sample readily identifying different measuring result by color, the probability reducing error is distinguished by color simultaneously.
With reference to shown in Fig. 2, quantitative determination example, absorbance and color are directly proportional.Absorbance is bigger, and color is deeper.
As Fig. 2, AFP (alpha-fetoprotein) measurement, this is a quantitative test, and SD1, SD2, SD3, SD4, SD5, SD6, SD7 are concentration standard items from low to high.Standard items color gradually deepens, description standard product place be to.The measurement of sample is also believable below.
Above content be with reference to specific embodiment made for the present invention further describe it is impossible to assert the present invention be embodied as be confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (8)
1. in a kind of immunizing dose adjust automatically Show Color method, including：Carry out the hardware device of pattern detection, connect the host computer receiving hardware device measurement data, the outlet terminal of display host computer result of calculation is it is characterised in that described hardware device includes spectrophotometric analysis instrument（5）, its method is：By spectrophotometric analysis instrument（5）Detect the absorbance of each sample of sample, and correspondence shows in outlet terminal.
2. in immunizing dose as claimed in claim 1 the method for adjust automatically Show Color it is characterised in that host computer result of calculation that described outlet terminal shows is to carry out each sample reduction of sample and corresponding parameter shows.
3. in immunizing dose as claimed in claim 2 the method for adjust automatically Show Color it is characterised in that described spectrophotometric analysis instrument（5）The sample absorbance recording is bigger, and the sample Show Color of corresponding reduction is deeper, shows white when absorbance angle value is 0.
4. in the immunizing dose as described in claim 1,2 or 3 method of adjust automatically Show Color it is characterised in that described spectrophotometric analysis instrument（5）Structure include for produce irradiate sample standard light light source（1）, towards sample carry out leaded light detection microscope group（3）And convert optical signals to electric signal and analyze the signal detection module of absorbance（4）.
5. in immunizing dose as claimed in claim 4 the method for adjust automatically Show Color it is characterised in that described spectrophotometric analysis instrument（5）Circuit is coupled with described host computer.
6. in immunizing dose as claimed in claim 4 the method for adjust automatically Show Color it is characterised in that described spectrophotometric analysis instrument（5）It is the spectrophotometric analysis instrument measuring luminous intensity based on chemiluminescence.
7. in immunizing dose as claimed in claim 4 the method for adjust automatically Show Color it is characterised in that described spectrophotometric analysis instrument（5）It is the spectrophotometric analysis instrument surveying shade based on Gold standard.
8. in immunizing dose as claimed in claim 4 the method for adjust automatically Show Color it is characterised in that described spectrophotometric analysis instrument（5）It is the spectrophotometric analysis instrument based on fluorescence measurement fluorescence intensity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241016.0A CN106405068A (en) | 2016-04-18 | 2016-04-18 | Method for automatically adjusting displayed color during immunoassay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241016.0A CN106405068A (en) | 2016-04-18 | 2016-04-18 | Method for automatically adjusting displayed color during immunoassay |
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| CN106405068A true CN106405068A (en) | 2017-02-15 |
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| CN201610241016.0A Pending CN106405068A (en) | 2016-04-18 | 2016-04-18 | Method for automatically adjusting displayed color during immunoassay |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60207040A (en) * | 1984-03-30 | 1985-10-18 | Shimadzu Corp | Analyzed result displaying device |
| JPH08117200A (en) * | 1994-10-24 | 1996-05-14 | Kitsusei Comtec Kk | Measuring instrument and its display |
| JP2006090734A (en) * | 2004-09-21 | 2006-04-06 | Fuji Photo Film Co Ltd | Measurement result display system, and program |
| JP3930450B2 (en) * | 2003-03-26 | 2007-06-13 | 富士通株式会社 | Analysis result visualization processing program |
| JP2008093147A (en) * | 2006-10-11 | 2008-04-24 | Matsushita Electric Ind Co Ltd | Blood testing apparatus |
| CN103491873A (en) * | 2011-04-21 | 2014-01-01 | 学校法人圣玛丽安娜医科大学 | Concentration-measurement device and concentration-measurement method |
| JP2014038055A (en) * | 2012-08-20 | 2014-02-27 | Hitachi High-Technologies Corp | Spectrophotometer |
| JP2014145594A (en) * | 2013-01-25 | 2014-08-14 | Terumo Corp | Blood glucose meter |
| CN105069321A (en) * | 2015-09-25 | 2015-11-18 | 成都金盘电子科大多媒体技术有限公司 | Use method of medical treatment and health information platform for doctor |
| CN204855517U (en) * | 2015-07-30 | 2015-12-09 | 聿新生物科技股份有限公司 | Biochemical detector with striking suggestion |
-
2016
- 2016-04-18 CN CN201610241016.0A patent/CN106405068A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60207040A (en) * | 1984-03-30 | 1985-10-18 | Shimadzu Corp | Analyzed result displaying device |
| JPH08117200A (en) * | 1994-10-24 | 1996-05-14 | Kitsusei Comtec Kk | Measuring instrument and its display |
| JP3930450B2 (en) * | 2003-03-26 | 2007-06-13 | 富士通株式会社 | Analysis result visualization processing program |
| JP2006090734A (en) * | 2004-09-21 | 2006-04-06 | Fuji Photo Film Co Ltd | Measurement result display system, and program |
| JP2008093147A (en) * | 2006-10-11 | 2008-04-24 | Matsushita Electric Ind Co Ltd | Blood testing apparatus |
| CN103491873A (en) * | 2011-04-21 | 2014-01-01 | 学校法人圣玛丽安娜医科大学 | Concentration-measurement device and concentration-measurement method |
| JP2014038055A (en) * | 2012-08-20 | 2014-02-27 | Hitachi High-Technologies Corp | Spectrophotometer |
| JP2014145594A (en) * | 2013-01-25 | 2014-08-14 | Terumo Corp | Blood glucose meter |
| CN204855517U (en) * | 2015-07-30 | 2015-12-09 | 聿新生物科技股份有限公司 | Biochemical detector with striking suggestion |
| CN105069321A (en) * | 2015-09-25 | 2015-11-18 | 成都金盘电子科大多媒体技术有限公司 | Use method of medical treatment and health information platform for doctor |
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Application publication date: 20170215 |