CN106399054A - Reaction chamber used for in-vitro diagnosis device and with cucurbit-shaped opening - Google Patents
Reaction chamber used for in-vitro diagnosis device and with cucurbit-shaped opening Download PDFInfo
- Publication number
- CN106399054A CN106399054A CN201611030774.4A CN201611030774A CN106399054A CN 106399054 A CN106399054 A CN 106399054A CN 201611030774 A CN201611030774 A CN 201611030774A CN 106399054 A CN106399054 A CN 106399054A
- Authority
- CN
- China
- Prior art keywords
- wall
- enrichment
- bin
- chamfered
- chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
The invention discloses a reaction chamber used for an in-vitro diagnosis device and with a cucurbit-shaped opening. The reaction chamber comprises a chamber body, the chamber body comprises a chamber room and a chamber bottom, the inner contour of the cross section of the upper end portion of the chamber room is a cucurbit-shaped contour formed by connecting a major-diameter arc segment and a minor-diameter arc segment, the upper sidewall of the chamber room has a cylindrical structure, the inner contour of the lower sidewall of the chamber room is an inverted truncated cone structure or bowl-shaped structure with large top and small bottom, and the chamber bottom is an arc bottom; and an enrichment chamfer wall extending from the upper sidewall to the lower end of the lower sidewall is chamfered on the sidewall of the chamber room, the lower end of the enrichment chamfer wall is arranged oblique to the middle axis of the chamber bottom, and an enrichment device is arranged on the lower sidewall of the chamber room. The reaction chamber has the following advantages: the reaction chamber has a simple structure and is convenient to use; the chamber bottom is the arc bottom, so vortex is convenient; the lower sidewall is the inverted truncated cone structure or the bowl-shaped structure, so buffer effect and convenient vortex are realized; and the wall thickness is consistent, so reaction reagents are uniformly heated, the enrichment distance is short, and the enrichment time is shortened, thereby the detection efficiency is improved.
Description
Technical field
The present invention relates to in-vitro diagnosis equipment detection reaction warehouse, especially relate to one kind and for in-vitro diagnosis equipment and carry
There is the reaction warehouse of shaped open.
Background technology
The target DNA of denier, also known as cell-free assay cloning system, can be expanded up to a million times by gene amplification, thus
Greatly improve the analysis to target DNA molecule and power of test, unique DNA can be detected or only contain one in every 90,000 cells
The sample of target DNA molecule, thus diagnostic field is rapidly developed in vitro, for detecting the diseases such as heredopathia, infectious disease.Often
In-vitro diagnosis the equipment such as instrument such as automatic chemiluminescence immunoassay system, biochemistry analyzer and gene amplification are combined into
Integrally carry out the diagnosis of multiple diseases or virus.According to statistics, now result in gene amplification and detect that the key factor of failure is to carry
Pure quality out of control and lead to the loss of target DNA or be contaminated, thus the purification after target DNA replicates is to directly affect gene to expand
Increase the key operation of reaction, directly affect the accuracy of disease detection result.The reaction warehouse used in purification step is used to obtain
Obtain the medical disposable material of purification or repeatable consumptive material, it is desirable to have receive the reaction compartment of mixture, in detection process, instead
Answer storehouse should meet the action docking of same self-reacting device equipment, meet types of functionality demand according to its operation program again and prevent
Pollution demand, and success rate and the accuracy high-quality purified being obtained with this and then guaranteeing amplified reaction.Existing reaction warehouse
Mixing is uneven, and wall cling phenomenon easily in reactant mixture, and the enrichment with magnetic bead time with target DNA is long, and then affects sample
The accuracy of detection.
Content of the invention
Present invention aim at providing a kind of reaction warehouse for in-vitro diagnosis equipment and with shaped open.
For achieving the above object, the present invention takes following technical proposals：
Reaction warehouse for in-vitro diagnosis equipment and with shaped open of the present invention, is provided with ring for fixing including top
Warehouse, described warehouse is made up of with the orlop being arranged on described bin lower end bin, the lubrication groove of described bin upper end cross section
Exterior feature is the Pear-Shaped profile being made up of large-diameter circular segmental arc and the linking of small-diameter circular segmental arc, and the upper side wall of bin is in cylindrical knot
Structure, the lower wall Internal periphery of bin is up big and down small inverted round stage structure or bowl structure, and described orlop is circular arc bottom；With institute
The enrichment extending from the downward side wall lower ends of upper side wall of cutting sth. askew is stated on the bin side wall of small-diameter circular segmental arc corresponding position cut sth. askew
Wall, the lower end of described enrichment chamfered wall is obliquely installed to orlop axis, and the enrichment inner surface of chamfered wall and outer surface are flat
Face structure, the angle α between enrichment chamfered wall and described orlop axis is 150～175 °, and the lower wall of bin is provided with
Enriching apparatus.
The bin lower wall longitudinal section outer contour relative with described enrichment chamfered wall is straight line or camber line.
On the bin side wall of described enrichment chamfered wall both sides, homocline cuts with from the extension of upper side wall downward side wall lower ends
Heating chamfered wall, each described heating inner surface of chamfered wall and outer surface are planar structure, heating chamfered wall outer surface with
Between enrichment chamfered wall, the angle β between outer surface is 80～90 °.
The wall thickness of described warehouse is 0.4～1.0mm.
The width of described enrichment chamfered wall is 4～8mm.
The invention has the advantages that structure is simple, easy to use；Orlop is circular arc bottom, is easy to be vortexed；Lower wall is inverted round stage
Structure or bowl structure, have cushioning effect, are easy to be vortexed, and according to liquid vortex principle, the reaction reagent flowing into orlop is vortexed
Fall again after rising, realizing being vortexed mixes；Enrichment chamfered wall and orlop axis angle α are definite value, the height of enrichment chamfered wall
The centre-to-centre spacing and large diameter circular arc of shaped open, small diameter arc between is proportionate；After fluid injection, can be directly to downside
Wall is heated or to heating chamfered wall heating, and wall thickness is consistent, and reaction reagent is heated evenly, and enrichment chamfered wall is obliquely installed,
Enrichment, apart from short, shortens enrichment time, and then improves detection efficiency.
Brief description
Fig. 1 is the structural representation that lower wall of the present invention is during inverted round stage structure.
Fig. 2 is the perspective view that lower wall of the present invention is during inverted round stage structure（Conceal enriching apparatus）.
Fig. 3 is the overlooking the structure diagram of Fig. 2.
Fig. 4 is the structural representation that lower wall of the present invention is during bowl structure.
Fig. 5 is the perspective view that lower wall of the present invention is during bowl structure（Conceal enriching apparatus）.
Fig. 6 is the structural representation that the present invention carries during heating chamfered wall.
Fig. 7 is the perspective view that the present invention carries during heating chamfered wall（Conceal enriching apparatus）.
Fig. 8 is the overlooking the structure diagram of Fig. 7.
Specific embodiment
As shown in Figure 1 and Figure 2, the reaction warehouse for in-vitro diagnosis equipment and with shaped open of the present invention, including
Top is provided with the warehouse of ring for fixing 1, and described warehouse includes the bin of open top setting and is arranged on described bin lower end
Orlop 2, warehouse 2 is circular arc bottom, is easy to be vortexed, and so that the useless capacity of bin is minimized, plays vortex in reaction reagent adding procedure
Effect；As shown in figure 3, the Internal periphery of described bin upper end cross section is to be made up of large diameter circular arc and small diameter arc linking
Pear-Shaped profile 4, that is, the upper shed of bin be shaped open；As shown in Figure 1 and Figure 2, bin is included in cylindrical upside
Wall 4 and the lower wall 5 being arranged on described upper side wall 4 lower end, described orlop 2 is arranged on the bottom of lower wall 5, with described minor diameter
Cutting sth. askew on the bin of circular arc opening corresponding position has the enrichment chamfered wall 6 extending from upper side wall 4 to lower wall 5 lower end, described
The lower end of enrichment chamfered wall 6 tilts to orlop 2 axis, and the inner surface of enrichment chamfered wall 6 and outer surface are planar structure；Institute
Stating lower wall 5 Internal periphery is up big and down small reverse frustoconic structure, the lower wall 5 with described enrichment chamfered wall 6 corresponding position
Longitudinal section outer contour is straight line；During actual manufacture, the Internal periphery of the lower wall 5 of bin can also be as shown in Figure 4, Figure 5
Up big and down small bowl structure, the lower wall 5 longitudinal section outer contour with described enrichment chamfered wall 6 corresponding position is camber line；Storehouse
The lower wall 5 of room has cushioning effect, is easy to be vortexed；Enriching apparatus 7 are provided with the lower wall 5 of bin, enriching apparatus 7 are can
The magnet piece of movement, enriching apparatus 7 can be arranged in enrichment chamfered wall 6, can also be arranged on position relative with being enriched with chamfered wall 6
Put in the bin lower wall 5 at place, can also be simultaneously in enrichment chamfered wall 6 and bin lower wall 5.
During actual manufacture, in order to heat conveniently, in homocline on the bin at enrichment chamfered wall 6 front side and rearward position
Cut with the heating chamfered wall 8 extending from upper side wall 4 to lower wall 5 lower end, each described heating inner surface of chamfered wall 8 and appearance
Face is planar structure, and between heating chamfered wall 8 outer surface and enrichment chamfered wall 6, the angle β between outer surface is 90 °.
During actual manufacture, the angle α between enrichment chamfered wall 6 and described orlop 2 axis is fixed value, is enriched with chamfered wall
Centre-to-centre spacing between 6 height and the large diameter circular arc of shaped open, small diameter arc is proportionate, and centre-to-centre spacing is bigger, rich
, closer to ring for fixing 1, that is, the height of enrichment chamfered wall 6 is bigger for the highest point of collection chamfered wall 6.As illustrated in figures 4-5, it is enriched with chamfered wall
Angle between 6 and orlop 2 axis is 168 °, between the large-diameter circular segmental arc of shaped open and small-diameter circular segmental arc in
The heart is 6mm away from the width for 6.3mm, being enriched with chamfered wall 6, and the distance between enrichment chamfered wall 6 top and ring for fixing 1 bottom are
7.6mm；As shown in figs 6-8, enrichment chamfered wall 6 and orlop 2 axis between angle α be 168 °, shaped open straight greatly
Centre-to-centre spacing between footpath arc section and small-diameter circular segmental arc is 7.3mm, and the width of enrichment chamfered wall 6 is 6mm, is enriched with chamfered wall 6
The distance between top and ring for fixing 1 bottom are 2.0mm.
When actually used, enriching apparatus 7 may move, and in fluid injection and blending process removes enriching apparatus 7, and magnetic bead reagent is rich
Again enriching apparatus 7 are moved to the outer surface of enrichment chamfered wall 6, during fluid injection, as shown in Fig. 1, Fig. 4, Fig. 6, liquid injection pipe 9 during collection
Fluid injection position is that on the axis that enrichment chamfered wall 6 middle and upper part is located, the fluid injection depth of liquid injection pipe 9 is true by the addition of reaction reagent
Fixed, the enriched chamfered wall of reaction reagent 6 flows into orlop 2, it is to avoid leads to wall built-up and mixing non-uniform phenomenon because of fluid injection position, carries
High accuracy；The wall thickness of bin is 0.7mm, and wall thickness is consistent, is heated evenly, and is easy to conduct heat；It is located at the storehouse of enrichment chamfered wall 6 both sides
Room upper side wall 4 has keeps away position effect, prevents reaction reagent from going out bin during being vortexed；Bin lower wall outer surface is provided with
Enriching apparatus 7, be easy to magnetic bead reagent the enrichment of enrichment magnetic bead reagent when directly along enrichment chamfered wall 6 longitudinally absorption table in it
Face, that is, magnetic bead reagent is in the linear structure of one fixed width.
The operation principle of the present invention is summarized as follows：
When actually used, add magnetic bead reagent first into bin, into bin, then inject reaction reagent, the note of reaction reagent
Liquid level flows downwardly into orlop 2 setting in the top of enrichment chamfered wall 6 inner surface, reaction reagent along enrichment chamfered wall 6 inner surface,
Orlop 2 is arc point bottom, and according to vortex principle, the reaction reagent flowing into orlop 2 is vortexed upwards to lower wall 5 inner surface, then
Rise to upper side wall 4, then reaction reagent falls into orlop 2 in the presence of self gravitation again, realize reaction reagent and magnetic bead
The mixing of reagent, can be repeated imbibition, fluid injection action, make reaction mix homogeneously, fluid injection post-heating lower wall 5 or heating tiltedly
Cut wall 8 reactant mixture is heated, provide reaction required heat.
After reaction terminates, with the enriching apparatus 7 being arranged in enrichment chamfered wall 6, magnetic bead is enriched with, with enrichment 30
After～70 seconds, magnetic bead reagent can adsorb completely in the enrichment chamfered wall 6 of enriching apparatus 7 corresponding position, with pipette 10
Carry out slow imbibition until suctioning out the solution at orlop 2 position completely, you can realize the experimental implementation of magnetic bead reagent enrichment.
Claims (6)
1. a kind of reaction warehouse for in-vitro diagnosis equipment and with shaped open, is provided with ring for fixing including top（1）'s
Warehouse, described warehouse is made up of with the orlop being arranged on described bin lower end bin（2）It is characterised in that：Described bin upper end
The Internal periphery of cross section is the Pear-Shaped profile being made up of large-diameter circular segmental arc and the linking of small-diameter circular segmental arc（3）, bin
Upper side wall（4）In cylindrical structural, the lower wall of bin（5）Internal periphery is up big and down small inverted round stage structure or bowl structure,
Described orlop（2）For circular arc bottom；Have from upper side wall with cutting sth. askew on the bin side wall of described small-diameter circular segmental arc corresponding position（4）
To lower wall（5）The enrichment chamfered wall that lower end extends（6）, described enrichment chamfered wall（6）Lower end to orlop（2）Axis tilts
Setting, is enriched with chamfered wall（6）Inner surface and outer surface be planar structure, be enriched with chamfered wall（6）With described orlop（2）Axis
Angle α between line is 150～175 °, and the lower wall of bin is provided with enriching apparatus（7）.
2. according to claim 1 be used for in-vitro diagnosis equipment and carry shaped open reaction warehouse it is characterised in that：With
Described enrichment chamfered wall（6）Relative bin lower wall（5）Longitudinal section outer contour is straight line or camber line.
3. the reaction warehouse for in-vitro diagnosis equipment and with shaped open according to claim 1 or claim 2, its feature exists
In：Positioned at described enrichment chamfered wall（6）On the bin side wall of both sides, homocline cuts with from upper side wall（4）To lower wall（5）Lower end is prolonged
The heating chamfered wall stretched（8）, each described heating chamfered wall（8）Inner surface and outer surface be planar structure, heating is cut sth. askew
Wall（8）Outer surface and enrichment chamfered wall（6）Between angle β between outer surface be 80～90 °.
4. the reaction warehouse for in-vitro diagnosis equipment and with shaped open according to claim 1 or claim 2, its feature exists
In：The wall thickness of described warehouse is 0.4～1.0mm.
5. the reaction warehouse for in-vitro diagnosis equipment and with shaped open according to claim 1 or claim 2, described enrichment is oblique
Cut wall（6）Width be 4～8mm.
6. according to claim 3 be used for in-vitro diagnosis equipment and carry shaped open reaction warehouse it is characterised in that：Institute
State enrichment chamfered wall（6）Width be 4～8mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611030774.4A CN106399054B (en) | 2016-11-22 | 2016-11-22 | For in-vitro diagnosis equipment and with the reaction warehouse of shaped open |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611030774.4A CN106399054B (en) | 2016-11-22 | 2016-11-22 | For in-vitro diagnosis equipment and with the reaction warehouse of shaped open |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106399054A true CN106399054A (en) | 2017-02-15 |
| CN106399054B CN106399054B (en) | 2019-01-04 |
Family
ID=58081630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611030774.4A Active CN106399054B (en) | 2016-11-22 | 2016-11-22 | For in-vitro diagnosis equipment and with the reaction warehouse of shaped open |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106399054B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998050158A1 (en) * | 1997-05-02 | 1998-11-12 | Gen-Probe Incorporated | Reaction receptacle apparatus |
| US20050271550A1 (en) * | 2004-06-08 | 2005-12-08 | Mark Talmer | Tapered cuvette and method of collecting magnetic particles |
| CN101869855A (en) * | 2010-05-31 | 2010-10-27 | 嘉兴凯实生物科技有限公司 | Nucleic acid separation and detection method and test tube suitable for method |
| JP5057228B2 (en) * | 2007-01-26 | 2012-10-24 | 栄研化学株式会社 | Reaction or detection tube container and reagent kit including the container |
| CN203379905U (en) * | 2013-06-21 | 2014-01-08 | 台湾圆点奈米技术股份有限公司 | Reagent tank and kit thereof |
| CN103966076A (en) * | 2014-05-16 | 2014-08-06 | 杭州奥盛仪器有限公司 | Experimental box for purifying biological extracts |
| CN206266575U (en) * | 2016-11-22 | 2017-06-20 | 安图实验仪器(郑州)有限公司 | For in-vitro diagnosis equipment and with shaped open reaction warehouse |
-
2016
- 2016-11-22 CN CN201611030774.4A patent/CN106399054B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998050158A1 (en) * | 1997-05-02 | 1998-11-12 | Gen-Probe Incorporated | Reaction receptacle apparatus |
| US20050271550A1 (en) * | 2004-06-08 | 2005-12-08 | Mark Talmer | Tapered cuvette and method of collecting magnetic particles |
| JP5057228B2 (en) * | 2007-01-26 | 2012-10-24 | 栄研化学株式会社 | Reaction or detection tube container and reagent kit including the container |
| CN101869855A (en) * | 2010-05-31 | 2010-10-27 | 嘉兴凯实生物科技有限公司 | Nucleic acid separation and detection method and test tube suitable for method |
| CN203379905U (en) * | 2013-06-21 | 2014-01-08 | 台湾圆点奈米技术股份有限公司 | Reagent tank and kit thereof |
| CN103966076A (en) * | 2014-05-16 | 2014-08-06 | 杭州奥盛仪器有限公司 | Experimental box for purifying biological extracts |
| CN206266575U (en) * | 2016-11-22 | 2017-06-20 | 安图实验仪器(郑州)有限公司 | For in-vitro diagnosis equipment and with shaped open reaction warehouse |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106399054B (en) | 2019-01-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102072949B (en) | Integrated quantitative sampling and reagent adding device | |
| ES2617258T3 (en) | Preparation of generic samples | |
| CN203639460U (en) | Fully enclosed integrated nucleic acid amplification and detection tube | |
| CN112852626A (en) | Integrated sample detection device and detection method thereof | |
| CN106399054A (en) | Reaction chamber used for in-vitro diagnosis device and with cucurbit-shaped opening | |
| CN206266575U (en) | For in-vitro diagnosis equipment and with shaped open reaction warehouse | |
| CN206204280U (en) | It is suitable to the single nucleic acid extraction box of diagnostic nucleic acid system | |
| ES2933132T3 (en) | Procedure to detect a cellular immune response | |
| CN206204281U (en) | For in-vitro diagnosis equipment and with square aperture reaction warehouse | |
| CN206204282U (en) | It is suitable to the double nucleic acid extraction box of diagnostic nucleic acid system | |
| BR112020001252A2 (en) | modules for transferring magnetic blood cells, automated system comprising them and method for extracting nucleic acids using the same | |
| CN106281977B (en) | Double nucleic acid extraction box suitable for diagnostic nucleic acid system | |
| CN106367310B (en) | For in-vitro diagnosis equipment and with square aperture reaction warehouse | |
| CN206204279U (en) | For in-vitro diagnosis equipment and with water-drop-shaped opening reaction warehouse | |
| US10590378B2 (en) | Cell separation chip and method for separating cells using same | |
| CN206266619U (en) | For in-vitro diagnosis equipment and with class triangle open mouth reaction warehouse | |
| CN206204278U (en) | For the reaction warehouse of in-vitro diagnosis equipment | |
| CN111167528B (en) | Reaction chamber of micro-fluidic chip | |
| CN206266574U (en) | It is suitable to the reaction warehouse of in-vitro diagnosis equipment | |
| CN107983424B (en) | Liquid drop biological analysis chip and application and use method thereof | |
| CN216513849U (en) | Closed nucleic acid detection device | |
| CN216427262U (en) | Nucleic acid extraction box | |
| CN211987210U (en) | High-flux cross-contamination-prevention solid phase extraction device | |
| EP3533521A1 (en) | Pipette tip and pipette system | |
| CN209715942U (en) | Sleeve needle suitable for magnetic bead cleaning system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |