CN106386595B - A kind of micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis - Google Patents
A kind of micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis Download PDFInfo
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- CN106386595B CN106386595B CN201610798468.9A CN201610798468A CN106386595B CN 106386595 B CN106386595 B CN 106386595B CN 201610798468 A CN201610798468 A CN 201610798468A CN 106386595 B CN106386595 B CN 106386595B
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- artemia
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- 238000000034 method Methods 0.000 title claims abstract description 40
- 241000058338 Macrobrachium nipponense Species 0.000 title claims abstract description 38
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 23
- 230000035764 nutrition Effects 0.000 title claims abstract description 23
- 230000001737 promoting effect Effects 0.000 title claims abstract description 12
- 241001247197 Cephalocarida Species 0.000 claims abstract description 53
- 239000011669 selenium Substances 0.000 claims abstract description 28
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 27
- 235000021323 fish oil Nutrition 0.000 claims abstract description 27
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 27
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 25
- 230000001804 emulsifying effect Effects 0.000 claims abstract description 25
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 23
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052802 copper Inorganic materials 0.000 claims abstract description 18
- 239000010949 copper Substances 0.000 claims abstract description 18
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 230000003014 reinforcing effect Effects 0.000 claims abstract description 17
- 239000011701 zinc Substances 0.000 claims abstract description 17
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 17
- 239000011573 trace mineral Substances 0.000 claims abstract description 16
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 16
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 10
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 10
- 238000005728 strengthening Methods 0.000 claims abstract description 9
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 238000012423 maintenance Methods 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 8
- 241000238017 Astacoidea Species 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 description 6
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 239000011785 micronutrient Substances 0.000 description 4
- 235000013369 micronutrients Nutrition 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- 241000700141 Rotifera Species 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000002787 reinforcement Effects 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 235000003715 nutritional status Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of micro-element nutrition intensifying methods for promoting Macrobrachium nipponensis paedomorphosis, use highly concentrated chlorella for Rich in Trace Element carrier, then prepare certain density copper-bath and solution of zinc sulfate；The enrichment for carrying out trace copper and zinc to chlorella using the above solution, chlorella, which is put into cultivation, after enrichment has in the sink of artemia, while emulsifying fish oil is added and yeast selenium strengthens artemia；Feeding finally is carried out to the Macrobrachium nipponensis young using the artemia after strengthening.The micro-element nutrition intensifying method provided by the invention for promoting Macrobrachium nipponensis paedomorphosis can be good at carrying out microelement reinforcing to the feeding artemia of the pond crayfish young, while the artemia feeding Macrobrachium nipponensis young after being strengthened using microelement can be obviously improved young survival rate.
Description
Technical field
The present invention relates to a kind of fortification methods, more particularly to a kind of micro member for promoting Macrobrachium nipponensis paedomorphosis
The method that element strengthens artemia.
Background technique
The artificial breeding Macrobrachium nipponensis young needs a large amount of living body bait, and the artemia of artificial culture is generally used as Shrimp waste young
The living body bait of body.During artificial breeding, the nutrient balance of the open-mouthed bait of stage of development plays the seed yield in later period
To key effect.Prothetic group and metal-binding sites of the microelement as some enzymes are able to maintain that skeleton development, and body is promoted to exempt from
Epidemic disease function is normally carried out with holding metabolism.This laboratory research shows copper in the artemia of artificial culture, zinc and Se content
Lower than the requirement for not being able to satisfy Macrobrachium nipponensis paedomorphosis.Compared to natural water area, the Macrobrachium nipponensis young is in artificial breeding ring
The living body bait type ingested under border is limited, and practice production is often neglected usually to feeding after living body bait wheel animalcule fortification
The fortification of microelement is omited.
At present there are many ways to fortification artemia, cost and effect are also had nothing in common with each other, to artemia feed Micro Algae,
Yeast and fish oil are relatively conventional schedule of reinforcements, and existing intensifying method is limited only to supplement rouge in living body bait wheel animalcule
Fat acid and vitamin, often have ignored the supplement of external source microelement.Copper, zinc and selenium are all water-soluble substances, and artemia can not
Absorb microelement in water body.So far, the method for fatty acid and vitamin enrichment artemia can be not applied directly to trace quantity mineral member
Element, and the reinforcing artemia method of water soluble trace elements is not yet established.So artemia microelement reinforcement technique is found, with full
Sufficient Macrobrachium nipponensis paedomorphosis demand, the problem of becoming urgent need to resolve.
Summary of the invention
It is an object of the invention to provide a kind of promotion Macrobrachium nipponensis young to overcome the above the deficiencies in the prior art
The micro-element nutrition intensifying method of development, by, come Rich in Trace Element, being enriched with by utilization micro- using highly enriched chlorella
Chlorella after secondary element further strengthens artemia to artemia feeding, while using emulsifying fish oil and yeast selenium directly to artemia
Strengthened, so that artemia has reached full nutritional status required for Macrobrachium nipponensis paedomorphosis, it being capable of high degree promotion day
The growth and development of this pond crayfish young.
Technical scheme is as follows:
It is a kind of promote Macrobrachium nipponensis paedomorphosis micro-element nutrition intensifying method, specific method the following steps are included:
Step 1 uses highly concentrated chlorella for Rich in Trace Element carrier；
Step 2, compound concentration are 500-1000 mg L−1Copper-bath and concentration be 200-500 mg L-1Sulphur
Sour zinc solution；
Step 3 takes the prepared copper-bath of highly concentrated chlorella addition step 2 of step 1 and zinc sulfate molten
Rich in Trace Element copper and zinc are carried out in the mixed solution of liquid；
Step 4 takes out the chlorella after step 2 Rich in Trace Element copper and zinc；It is put into the sink that cultivation has artemia
In, while emulsifying fish oil is added and yeast selenium strengthens artemia；
Step 5 carries out feeding to the Macrobrachium nipponensis young using the artemia after reinforcing.
Further, the micro-element nutrition intensifying method of the described promotion Macrobrachium nipponensis paedomorphosis is matched in step 1
The weight in wet base for setting chlorella is 7 × 1010 cells g−1。
Further, the micro-element nutrition intensifying method of the described promotion Macrobrachium nipponensis paedomorphosis, will in step 3
10 g chlorellas are added in the mixed liquor of 3-5ml copper-bath and 3-5ml solution of zinc sulfate.
Further, the micro-element nutrition intensifying method of the described promotion Macrobrachium nipponensis paedomorphosis, it is rich in step 3
Integrate the enrichment time of trace copper and zinc as 1-3h.
Further, the micro-element nutrition intensifying method of the described promotion Macrobrachium nipponensis paedomorphosis is strong in step 4
Change temperature and is greater than 6 mg/L, salinity 20 ‰ for 28 ± 0.5 DEG C, pH 7.8-8.2, dissolved oxygen.
Further, the micro-element nutrition intensifying method of the described promotion Macrobrachium nipponensis paedomorphosis, cream in step 4
The additional amount of change type fish oil is 2mg/L.
Further, the micro-element nutrition intensifying method of the described promotion Macrobrachium nipponensis paedomorphosis, ferment in step 4
The additional amount of female selenium is 2mg/L.
Further, the micro-element nutrition intensifying method of the described promotion Macrobrachium nipponensis paedomorphosis is strong in step 4
The change time is 8-12h.
Further, the micro-element nutrition intensifying method of the described promotion Macrobrachium nipponensis paedomorphosis, in step 4 plus
Enter the process that emulsifying fish oil and yeast selenium strengthen artemia to carry out in two stages, the first stage is that will be passed through in sink
System temperature is simultaneously warming up to 30-32 DEG C by oxygen, keeps 1-2h, emulsifying fish oil is then added, emulsifying fish oil and water is added
The mass volume ratio of slot culturing liquid is 1mg/L, keeps 1-2h, then adds yeast selenium, keeps 1-2h, the amount of yeast selenium is added
It is identical as the amount of emulsifying fish oil；Then system temperature is reduced to 15-18 DEG C, 8-10h is kept to keep into second stage
Emulsifying fish oil and yeast selenium, the emulsifying fish oil of addition and the amount of yeast selenium is added under conditions of being 15-18 DEG C in system temperature
Mass volume ratio with sink cultivation liquid system is 1mg/L, and maintenance system dissolved oxygen amount is greater than 6mg/L, pH7.8- after addition
8.2, sink culturing liquid system temperature is risen to 30 DEG C in 3-4h by salinity 20 ‰, keeps 1-2h, is strengthened and is completed.
Enrichment of the present invention by using chlorella to microelements such as copper and zinc, using the chlorella after enrichment to artemia
Carry out feeding reinforcing, greatly improve the content of microelement in artemia body, at the same using by the way of reinforcing artemia stage by stage into
One step improves the strengthening effect of artemia nutrient, carries out feeding to the Macrobrachium nipponensis young using the artemia after reinforcing, can
It is obviously improved the survival rate of the pond crayfish young.
Detailed description of the invention
Fig. 1 is control group and the artemia micronutrient levels comparison diagram after reinforcing in the embodiment of the present invention 1；
Fig. 2 is control group and the artemia micronutrient levels comparison diagram after reinforcing in the embodiment of the present invention 2；
It is the Macrobrachium nipponensis young control group strengthened and the young survival rate pair after reinforcing that Fig. 3, which is in the embodiment of the present invention 3,
Than figure.
Specific embodiment:
Embodiment 1
It is a kind of promote Macrobrachium nipponensis paedomorphosis micro-element nutrition intensifying method, specific method the following steps are included:
Step 1 uses highly concentrated chlorella for Rich in Trace Element carrier, and wherein the weight in wet base of chlorella is 7 × 1010
cells g−1；
Step 2, compound concentration are 1000 mg L−1Copper-bath and concentration be 500 mg L-1Zinc sulfate it is molten
Liquid；
Step 3 takes 10 g of highly concentrated chlorella of step 1 that the prepared 3 ml copper-bath of step 2 is added
With progress Rich in Trace Element copper and zinc in the mixed solution of 3 ml solution of zinc sulfate, it is enriched with the seawater of 1-3h, survival or death
Chlorella can be enriched with appropriate concentration trace copper and zinc；
Step 4 takes out the chlorella after step 2 Rich in Trace Element copper and zinc；It is put into the sink that cultivation has artemia
In, while emulsifying fish oil being added and yeast selenium strengthens artemia, strengthening process temperature is 28 ± 0.5 DEG C, pH 7.8-
8.2, dissolved oxygen is greater than 6 mg/L, salinity 20 ‰, and the amount of emulsifying fish oil and yeast selenium and the mass body of sink culturing liquid is added
Product ratio is 2mg/L, enhanced time 8-12h.
Nutrient measurement is carried out to the artemia after above strengthen, while being compared with the control group that do not strengthened,
The result is shown in Figure 1, it can be seen that the artemia copper coin cellulose content after reinforcing has reached 35(μ g/g dry matter) left and right, do not strengthen
3 times or more of control group content, the content of Zn-ef ficiency nearly 60(μ g/g dry matter), it is 2 times of the control group content that do not strengthen
More than, and the content of selenium element is not detected in the control group that do not strengthen, it can obviously be detected in the artemia after reinforcing
To selenium element content.
Embodiment 2
It is a kind of promote Macrobrachium nipponensis paedomorphosis micro-element nutrition intensifying method, specific method the following steps are included:
Step 1 uses highly concentrated chlorella for Rich in Trace Element carrier, and wherein the weight in wet base of chlorella is 7 × 1010
cells g−1；
Step 2, compound concentration are 500 mg L−1Copper-bath and concentration be 200 mg L-1Solution of zinc sulfate；
Step 3, take step 1 10 g of highly concentrated chlorella be added the prepared 5 ml copper-bath of step 2 and
Rich in Trace Element copper and zinc are carried out in the mixed solution of 5 ml solution of zinc sulfate, is enriched with 1-3h, and survival or dead seawater are small
Ball algae can be enriched with appropriate concentration trace copper and zinc；
Step 4 takes out the chlorella after step 2 Rich in Trace Element copper and zinc；It is put into the sink that cultivation has artemia
In, while emulsifying fish oil being added and yeast selenium strengthens artemia, the process of reinforcing carries out in two stages, the first stage
Will to be passed through oxygen in sink and system temperature is warming up to 30-32 DEG C, 1-2h is kept, emulsifying fish oil is then added, is added
The mass volume ratio of emulsifying fish oil and sink culturing liquid is 1mg/L, keeps 1-2h, then adds yeast selenium, keeps 1-2h,
The amount that yeast selenium is added is identical as the amount of emulsifying fish oil；Then system temperature is reduced to 15-18 DEG C, keeps 8-10h, into the
Emulsifying fish oil and yeast selenium, the emulsifying fish oil of addition is added under conditions of keeping system temperature to be 15-18 DEG C in two-stage
Mass volume ratio with amount and sink the cultivation liquid system of yeast selenium is 1mg/L, and maintenance system dissolved oxygen amount is greater than after addition
Sink culturing liquid system temperature is risen to 30 DEG C in 3-4h by 6mg/L, pH7.8-8.2, salinity 20 ‰, is kept 1-2h, has been strengthened
At.
Nutrient detection is carried out for the artemia after strengthening above, and is compared with the control group strengthened is not done, is tied
Fruit sees Fig. 2, it can be seen that the artemia copper coin cellulose content after reinforcing has been more than 40(μ g/g dry matter), be 4 times of control group with
On, artemia Zn-ef ficiency content has reached 70(μ g/g dry matter after reinforcing) more than, it is 3 times of the control group that do not strengthen or more, and
The content of selenium is also more obvious.
The present embodiment compared with Example 1, is changed, wherein this reality on intensifying method of the microelement to artemia
It applies example to strengthen artemia using two stages, by final result as can be seen that after the present embodiment reinforcing in artemia body
Micronutrient levels is apparently higher than the micronutrient levels after strengthening in the way of embodiment 1.Pass through two in the present embodiment
Stage strengthens artemia, improves absorption of the artemia to nutrient, increases artemia endotrophic element after strengthening
Content improves quality, can preferably strengthen as nutrient enrichment fodder to the Macrobrachium nipponensis young.
Embodiment 3
It is daily to the Macrobrachium nipponensis young after artemia is strengthened by method described in above embodiments in industrial seedling rearing
20-30/L of artemia is fed, is fed altogether 22 days.The development condition of the young shows to seek compared with control group is to feed reinforcing artemia
Supporting the artemia after strengthening has significant facilitation to the survival rate of the Macrobrachium nipponensis young, can promote the molt metamorphosis of the young,
And then reduce the generation of freshwater shrimp young autotomy phenomenon.As shown in figure 3, the young survival rate after strengthening is relative to the control to strengthen
Group survival rate improves 15% or more.
Claims (7)
1. a kind of micro-element nutrition intensifying method for promoting Macrobrachium nipponensis paedomorphosis, which is characterized in that specific method includes
Following steps:
Step 1 uses highly concentrated chlorella for Rich in Trace Element carrier；
Step 2, compound concentration are 500-1000 mg L−1Copper-bath and concentration be 200-500 mg L-1Zinc sulfate
Solution；
Step 3 takes the highly concentrated chlorella of step 1 that the prepared copper-bath of step 2 and solution of zinc sulfate is added
Rich in Trace Element copper and zinc are carried out in mixed solution；
Step 4 takes out the chlorella after step 3 Rich in Trace Element copper and zinc；Being put into cultivation has in the sink of artemia, together
When emulsifying fish oil and yeast selenium is added artemia is strengthened；
Step 5 carries out feeding to the Macrobrachium nipponensis young using the artemia after reinforcing；
The weight in wet base that chlorella is configured in step 1 is 7 × 1010 cells g−1；Emulsifying fish oil and yeast selenium are added in step 4
The process strengthened to artemia carries out in two stages, and the first stage is that will be passed through oxygen in sink and system temperature heats up
To 30-32 DEG C, 1-2h is kept, emulsifying fish oil is then added, the mass volume ratio of emulsifying fish oil and sink culturing liquid is added
For 1mg/L, 1-2h is kept, then adds yeast selenium, 1-2h is kept, the amount of yeast selenium and the amount phase of emulsifying fish oil is added
Together；Then system temperature is reduced to 15-18 DEG C, keeps 8-10h, into second stage, system temperature is being kept to be 15-18 DEG C
Under the conditions of emulsifying fish oil and yeast selenium, the matter of the emulsifying fish oil of addition and the amount of yeast selenium and sink cultivation liquid system is added
Measuring volume ratio is 1mg/L, and maintenance system dissolved oxygen amount is greater than 6mg/L after addition, and pH7.8-8.2, salinity 20 ‰ will in 3-4h
Sink culturing liquid system temperature rises to 30 DEG C, keeps 1-2h, strengthens and completes.
2. the micro-element nutrition intensifying method according to claim 1 for promoting Macrobrachium nipponensis paedomorphosis, feature exist
In 10 g chlorellas are added in the mixed liquor of 3-5ml copper-bath and 3-5ml solution of zinc sulfate in step 3.
3. the micro-element nutrition intensifying method according to claim 1 for promoting Macrobrachium nipponensis paedomorphosis, feature exist
In the enrichment time of Rich in Trace Element copper and zinc is 1-3h in step 3.
4. the micro-element nutrition intensifying method according to claim 1 for promoting Macrobrachium nipponensis paedomorphosis, feature exist
In strengthening temperature in step 4 is 28 ± 0.5 DEG C, pH 7.8-8.2, dissolved oxygen greater than 6 mg/L, salinity 20 ‰.
5. the micro-element nutrition intensifying method according to claim 1 for promoting Macrobrachium nipponensis paedomorphosis, feature exist
In the additional amount of emulsifying fish oil is 2mg/L in step 4.
6. the micro-element nutrition intensifying method according to claim 1 for promoting Macrobrachium nipponensis paedomorphosis, feature exist
In the additional amount of yeast selenium is 2mg/L in step 4.
7. the micro-element nutrition intensifying method according to claim 5 or 6 for promoting Macrobrachium nipponensis paedomorphosis, feature
It is, enhanced time is 8-12h in step 4.
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| EP1049385B1 (en) * | 1998-01-21 | 2003-06-18 | University of Maryland Biotechnology Institute | Methods for the enrichment of live feed for fish larvae with docosahexaenoic acid |
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