CN106801069A - Nano selenium and preparation method thereof - Google Patents
Nano selenium and preparation method thereof Download PDFInfo
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- CN106801069A CN106801069A CN201710046335.0A CN201710046335A CN106801069A CN 106801069 A CN106801069 A CN 106801069A CN 201710046335 A CN201710046335 A CN 201710046335A CN 106801069 A CN106801069 A CN 106801069A
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- 239000011669 selenium Substances 0.000 title claims abstract description 88
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 71
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims description 69
- 229940091258 selenium supplement Drugs 0.000 claims abstract description 70
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 38
- 235000013379 molasses Nutrition 0.000 claims abstract description 36
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 28
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 28
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 28
- 239000011781 sodium selenite Substances 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 19
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- 235000013405 beer Nutrition 0.000 claims abstract description 10
- 150000003839 salts Chemical class 0.000 claims abstract description 9
- 239000002921 fermentation waste Substances 0.000 claims abstract description 8
- 230000000050 nutritive effect Effects 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract 3
- 229940088594 vitamin Drugs 0.000 claims description 13
- 235000013343 vitamin Nutrition 0.000 claims description 13
- 229930003231 vitamin Natural products 0.000 claims description 13
- 239000011782 vitamin Substances 0.000 claims description 13
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 13
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 9
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 7
- 239000012467 final product Substances 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 2
- 239000002244 precipitate Substances 0.000 abstract description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 210000005253 yeast cell Anatomy 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 9
- 230000012010 growth Effects 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000002699 waste material Substances 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 229940044197 ammonium sulfate Drugs 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 150000003342 selenium Chemical class 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 230000007096 poisonous effect Effects 0.000 description 2
- 241001313855 Bletilla Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002389 environmental scanning electron microscopy Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B19/00—Selenium; Tellurium; Compounds thereof
- C01B19/02—Elemental selenium or tellurium
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/01—Particle morphology depicted by an image
- C01P2004/03—Particle morphology depicted by an image obtained by SEM
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/60—Particles characterised by their size
- C01P2004/64—Nanometer sized, i.e. from 1-100 nanometer
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Composite Materials (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biotechnology (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of nano-selenium, which comprises the following steps: (1) adding sodium selenite and nutritive salt into the molasses hydrolysate, and sterilizing to obtain a molasses culture medium, wherein the concentration of the sodium selenite in the molasses culture medium is 0.1-1.5 mg/mL; (2) centrifuging the beer fermentation waste liquid, and repeatedly cleaning the precipitate obtained by centrifuging to obtain yeast paste; (3) mixing the yeast paste obtained in the step (2) and the molasses culture medium obtained in the step (1) according to the ratio of 1: 3-1: mixing the materials according to a mass-volume ratio of 2g/mL, and fermenting for more than 2 hours at a temperature of 25-32 ℃ to obtain a fermentation liquid; (4) and (4) carrying out centrifugal separation on the fermentation liquor obtained in the step (3) to obtain yeast, and drying and crushing the yeast to obtain the nano-selenium. The preparation method has the advantages of low cost, short period and high preparation efficiency, and the prepared nano-selenium still can show active red nano-selenium at high temperature and has good stability.
Description
Technical field
The present invention relates to selenium preparing technical field, especially a kind of nanometer selenium and preparation method thereof.
Background technology
In brewing industry, 10000t beer is often produced, it will 100t fermented waste fluids are produced, wherein rich in a large amount of eggs
Bletilla vitamin, directly treatment will cause the substantial amounts of wasting of resources and environmental pollution, and developing one kind can reuse beer
The method of fermented waste fluid, makes it turn waste into wealth and is significant.
Selenium is a kind of important addition element in feed addictive, with the different physiological roles such as anti-oxidant, antitumor.Selenium exists
Often with+6 ,+4 in nature, based on 0, -2 four kinds of valence states, at present, and in China's feedstuff industry, because sodium selenite toxicity is high, peace
Complete smaller using scope, most area has been prohibited from using；And the yeast selenium based on the seleno-amino acids of-divalent, due to battalion
Value height, bioactivity is supported to be widely used well, but its cycle is long in process of production, and waste liquid is more, instrument and equipment high cost, greatly
Part small enterprise is difficult to maintain, and production is difficult.
The content of the invention
Based on this, a kind of system of nanometer selenium is provided it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part
Preparation Method, the preparation method has low cost, and cycle is short, unit Se content is high, and waste water is few.
To achieve the above object, the technical solution used in the present invention is：A kind of preparation method of nanometer selenium, including it is as follows
Step：
(1) sodium selenite, nutritive salt are added in molasses hydrolyzate, sterilizing obtains molasses culture medium, wherein, molasses training
The concentration for supporting sodium selenite in base is 0.1~1.5mg/mL；
(2) beer fermentation waste liquid is centrifuged, yeast paste is obtained；
(3) step (2) gained yeast paste and step (1) gained molasses culture medium are pressed 1：3~1：The quality volume of 2g/mL
Than being mixed, fermented more than 2 hours at being 25~32 DEG C in temperature, obtain zymotic fluid；
(4) step (3) gained zymotic fluid is centrifuged, obtains yeast thalline, yeast thalline is dried, powder
It is broken, obtain final product the nanometer selenium.
The preparation method of nanometer selenium of the present invention, is fermented using the concentration of beer fermentation waste liquid and prepares nanometer selenium, low cost,
Preparation efficiency is high, with larger economic benefit and environmental benefit.
Preferably, in step (1) the gained molasses culture medium, pol is 3~7 ° of Be '.
Sugar is to provide yeast growth metabolism one of main energy sources material, in the presence of enzyme, be oxidized discharge electronics and
Hydrogen ion, plays an important role in being nanometer selenium in reduction sodium selenite.Inventor attempts finding that sugared concentration is got over by a large amount of
Height, the Se content of conversion is higher, but unit yeast amount is gradually reduced, when sugar selects above-mentioned content, it is ensured that unit ferment
The unification of mother's amount and the Se content of conversion.
Preferably, in step (1) the gained molasses culture medium, pol is 5 ° of Be ', and the concentration of sodium selenite is 1mg/
mL。
When pol is above-mentioned content, the unification of the Se content of unit yeast amount and conversion is may further ensure that, make list
Position yeast amount and the Se content of conversion all reach highest.
The addition concentration of sodium selenite is the main factor for influenceing selenium conversion ratio, and addition concentration is higher, by sorption enhanced
Nanometer selenium it is more, but yeast cells adaptability and reducing substances therein are limited, and too high selenium concentration can cause yeast thin
Born of the same parents' self-dissolving is dead, and causes Se content to decline.By a large amount of trials, finding the sodium selenite of above-mentioned concentration can ensure selenium to inventor
Content is higher.When sodium selenite is above-mentioned concentration, the nanometer Se content highest of conversion can reach 11470ppm.
Preferably, in step (1) the gained molasses culture medium, nutritive salt includes ammonium sulfate, vitamin liquid, described
Mass percent of the ammonium sulfate in the molasses culture medium is 0.5~2.5%, and the vitamin liquid is trained in the molasses
It is 0.1~1% to support the mass percent in base.Nitrogen source during the nutritive salt afterfermentation required for Yeast Growth,
The nutriments such as vitamin, comprising the vitamin required for various Yeast Growths in the vitamin liquid.
Preferably, in the step (3), fermentation time is 3~12 hours.
Inventor has found in first 3 hours that unit Se content steeply rises, 3~12 in the research to fermentation time
Hour plateau is raised slowly to, this is probably there is substantial amounts of a certain reducing substances in itself in yeast cells, rapid
Poisonous sodium selenite is reduced to elemental selenium absorption in the cell, it is thin by yeast then due to the shortage of reducing substances
Slowly be enriched to sodium selenite in cell by the growth metabolism effect of born of the same parents.
Preferably, in the step (3), the mass volume ratio of yeast paste and molasses culture medium is 1：2.5g/mL.
Yeast paste addition is more, and the culture medium cost of unit consumption is lower, but yeast paste addition is too high, can make ferment
Female anoxic in the medium, causes anaerobic fermentation, and then produces alcohol, influences yield.
Preferably, in the step (3), fermented under conditions of being 4~8 in pH value.
Inventor is attempted by a large amount of, and after finding to be processed under different pH, not substantially, yeast cells is wrapped up for selenium concentration change
Effect is good, the stabilization in as feed addictive, however, it was found that declining afterwards as the increase Se content of pH first increases, pH is 4~8
When Se content entire change less, required during real attenuation low, be easy to production.
Preferably, it is to be fermented 3 hours under conditions of 7, temperature is 30 DEG C in pH value in the step (3).
Temperature is one of the key factor for influenceing all kinds of enzymatic activitys in yeast, enzyme serve in growth metabolism it is key,
The activities present of enzyme is different under different temperatures, and inventor is had found with the rising of temperature, the ferment after 30 DEG C by a large amount of trials
Female yield dramatic decrease, and Se content reaches peak at 30 DEG C, therefore, from 30 DEG C as fermentation temperature；Consider
Fermentation costs, therefore select 3 hours most preferably；Simultaneously when pH is 7, Se content highest, therefore selection pH is 7.
It is worth noting that, the supernatant that centrifugation goes out in the step (4), can reclaim, continue to be applied to molasses
In the preparation of culture medium, with further reduces cost, raw material availability is improved.
Meanwhile, the present invention also provides the nanometer selenium that a kind of above-mentioned preparation method is prepared.The nanometer selenium is by yeast
The active nano selenium of cell encapsulation, remains to the active red nano selenium of performance, good stability at high temperature.
Preferably, the nano granules of selenium size is 30~40nm.
Relative to prior art, beneficial effects of the present invention are：
Preparation method of the present invention prepares nanometer selenium using the concentration fermentation of beer fermentation waste liquid, and fermentation period is extremely short, and one
Determine that this link that sterilizes can be saved in degree, equipment requirement and cost input are considerably reduced, while resource reutilization, is
Environment alleviates pressure, turn waste into wealth, low cost, preparation efficiency it is high, more than sodium selenite Transformation efficiency is up to 90 %, highest unit
Se content 11470ppm, with larger economic benefit and environmental benefit.
The preparation-obtained nanometer selenium of the present invention remains to the active red nano selenium of performance, stability at high temperature
It is good, it is suitable to industrial production with application.
Brief description of the drawings
Fig. 1 is nanometer selenium real scene shooting figure of the present invention；
Fig. 2 is the broken nanometer selenium scanning electron microscope (SEM) photograph for extracting and obtaining；
Fig. 3 is yeast cells transmission electron microscope picture；
Fig. 4 is the influence figure that sugared concentration is converted to nanometer selenium；
Fig. 5 is the influence figure that sodium selenite addition is converted to nanometer selenium；
Fig. 6 is the influence figure that fermentation time is converted to nanometer selenium.
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with the drawings and specific embodiments pair
The present invention is described further.
Embodiment 1
A kind of embodiment of the preparation method of nanometer selenium of the present invention, the preparation method of nanometer selenium described in the present embodiment,
Comprise the following steps：
(1) sodium selenite, ammonium sulfate, vitamin are added in the molasses hydrolyzate that molasses mass percent is 10%
Liquid, 121 DEG C of high-temperature sterilizations, obtains molasses culture medium；In the culture medium, pol is 5 ° of Be ', and the concentration of sodium selenite is 1mg/
ML, the mass percent of ammonium sulfate is 2.5%, and the mass percent of vitamin liquid is 0.5%；
(2) beer fermentation waste liquid is separated under low speed centrifuge, is precipitated yeast paste；
(3) step (2) gained yeast paste and step (1) gained molasses culture medium are pressed (1：2.5) the quality volume of g/mL
Than being mixed, using 1mol/L salt acid for adjusting pH to 7,30 DEG C, continuing fermentation 3h are gradually heating to；Obtain zymotic fluid；
(4) thalline is obtained with 3000r/min centrifugations 5min, repeated washing washes away surface selenium salt for 3 times, is dried under high temperature, powder
It is broken, obtain final product the nanometer selenium.
The nanometer selenium that the preparation method as described in the present embodiment is prepared, is the active nano selenium wrapped up by yeast cells,
Granular size is 30nm.
Embodiment 2
A kind of embodiment of the preparation method of nanometer selenium of the present invention, the preparation method of nanometer selenium described in the present embodiment,
Comprise the following steps：
(1) sodium selenite, ammonium sulfate, vitamin are added in the molasses hydrolyzate that molasses mass percent is 10%
Liquid, 121 DEG C of high-temperature sterilizations, obtains molasses culture medium；In the culture medium, pol is 3 ° of Be ', and the concentration of sodium selenite is
0.1mg/mL, the mass percent of ammonium sulfate is 2%, and the mass percent of vitamin liquid is 0.1%；
(2) beer fermentation waste liquid is separated under low speed centrifuge, is precipitated yeast paste；
(3) step (2) gained yeast paste and step (1) gained molasses culture medium are pressed (1：2) mass volume ratio of g/mL
Mixed, using 1mol/L salt acid for adjusting pH to 4, be gradually heating to 25 DEG C, continuing fermentation 2h；Obtain zymotic fluid；
(4) thalline is obtained with 3000r/min centrifugations 5min, repeated washing washes away surface selenium salt for 3 times, is dried under high temperature, powder
It is broken, obtain final product the nanometer selenium.
The nanometer selenium that the preparation method as described in the present embodiment is prepared, is the active nano selenium wrapped up by yeast cells,
Granular size is 40nm.
Embodiment 3
A kind of embodiment of the preparation method of nanometer selenium of the present invention, the preparation method of nanometer selenium described in the present embodiment,
Comprise the following steps：
(1) sodium selenite, ammonium sulfate, vitamin are added in the molasses hydrolyzate that molasses mass percent is 10%
Liquid, 121 DEG C of high-temperature sterilizations, obtains molasses culture medium；In the culture medium, pol is 7 ° of Be ', and the concentration of sodium selenite is
1.5mg/mL, the mass percent of ammonium sulfate is 0.5%, and the mass percent of vitamin liquid is 1%；
(2) beer fermentation waste liquid is separated under low speed centrifuge, is precipitated yeast paste；
(3) step (2) gained yeast paste and step (1) gained molasses culture medium are pressed (1：0.5) the quality volume of g/mL
Than being mixed, using 1mol/L salt acid for adjusting pH to 8,32 DEG C, continuing fermentation 4h are gradually heating to；Obtain zymotic fluid；
(4) thalline is obtained with 3000r/min centrifugations 5min, repeated washing washes away surface selenium salt for 3 times, is dried under high temperature, powder
It is broken, obtain final product the nanometer selenium.
The nanometer selenium that the preparation method as described in the present embodiment is prepared, is the active nano selenium wrapped up by yeast cells,
Granular size is 35nm.
Embodiment 4
The present embodiment is using transmission electron microscope and ESEM to structure, the shape of the preparation-obtained nanometer selenium of embodiment 1~3
State and particle diameter are further observed, characterized, analyzed.
There are substantial amounts of compacted grains by around yeast cells in sign observation Fig. 3 and in kytoplasm, its yeast cells is big
Small about 3~4um, wherein granular size is about 30~40nm particles, and obtaining nanometer selenium by broken extraction precipitates by scanning
Electron microscopic observation, as shown in Fig. 2 its particle is about 40nm or so particles, it is consistent with the judgement in Fig. 3, dried by product
Crushing obtain product be placed in air place January after (design sketch such as Fig. 1) be cerise, be the feature face of red active nano selenium
Color, thus judges that the nanometer selenium prepared by this kind of method is active nano selenium.
Embodiment 5
Selection of the present embodiment to pol in nanometer selenium preparation method of the present invention, selenium addition, fermentation time is carried out
Research, method of the research using unitary variant is controlled, specific data is as shown in figures 4-6.
As seen from Figure 4, pol is higher, and the Se content of conversion is higher, but yeast amount is with the increase of pol, presents first
The trend subtracted after increasing, when pol is 5 ° of Be ', unit yeast amount is maximum.
As shown in Figure 5, with the increase of concentration of sodium selenite, the Se content of conversion is presented the trend for first increasing and subtracting afterwards, yeast
Amount slowly declines with the increase of concentration of sodium selenite, and when the addition of sodium selenite is 1mg/mL, the nanometer selenium of conversion contains
Amount highest, can reach 11470ppm, and it is unobvious that yeast amount declines again.
It will be appreciated from fig. 6 that inventor is in the research to fermentation time, find in first 3 hours, unit Se content is drastically gone up
Rise, plateau was raised slowly at 3~12 hours, this is probably there is substantial amounts of a certain reproducibility thing in itself in yeast cells
Matter, is rapidly being reduced to elemental selenium absorption in the cell by poisonous sodium selenite, then due to the shortage of reducing substances, leads to
Slowly be enriched to sodium selenite in cell by the growth metabolism effect for crossing yeast cells.Meanwhile, the amount of dry ferment is with fermentation
The increase of time has a slow downward trend, therefore fermentation time selects 3~12 hours, and now unit Se content is basically reached most
Big value, and amount of yeast decline is unobvious, while the fermentation time that Fig. 6 also further illustrates the method for the invention is short
Advantage.
It is last to should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent, without deviating from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of preparation method of nanometer selenium, it is characterised in that comprise the following steps：
(1) sodium selenite, nutritive salt are added in molasses hydrolyzate, sterilizing obtains molasses culture medium, wherein, molasses culture medium
The concentration of middle sodium selenite is 0.1~1.5mg/mL；
(2) beer fermentation waste liquid is centrifuged, yeast paste is obtained；
(3) step (2) gained yeast paste and step (1) gained molasses culture medium are pressed 1：3~1：The mass volume ratio of 2g/mL enters
Row mixing, ferments more than 2 hours at being 25~32 DEG C in temperature, obtains zymotic fluid；
(4) step (3) gained zymotic fluid is centrifuged, obtains yeast thalline, yeast thalline is dried, is crushed,
Obtain final product the nanometer selenium.
2. the preparation method of nanometer selenium as claimed in claim 1, it is characterised in that in step (1) the gained molasses culture medium,
Pol is 3~7 ° of Be '.
3. the preparation method of nanometer selenium as claimed in claim 2, it is characterised in that in step (1) the gained molasses culture medium,
Pol is 5 ° of Be ', and the concentration of sodium selenite is 1mg/mL.
4. as described in any one of claims 1 to 3 nanometer selenium preparation method, it is characterised in that the nutritive salt include sulfuric acid
Ammonium, vitamin liquid, mass percent of the ammonium sulfate in the molasses culture medium are 0.5~2.5%, the dimension life
Mass percent of the plain nutrient solution in the molasses culture medium is 0.1~1%.
5. the preparation method of nanometer selenium as claimed in claim 1, it is characterised in that in the step (3), fermentation time is 3~
12 hours.
6. the preparation method of nanometer selenium as claimed in claim 1, it is characterised in that in the step (3), yeast paste and molasses are trained
The mass volume ratio for supporting base is 1：2.5g/mL.
7. as described in claim 1 or 5 or 6 nanometer selenium preparation method, it is characterised in that in the step (3), be in pH value
Fermented under conditions of 4~8.
8. the preparation method of nanometer selenium as claimed in claim 7, it is characterised in that in the step (3), is 7, temperature in pH value
To be fermented 3 hours under conditions of 30 DEG C.
9. the nanometer selenium that a kind of preparation method of the nanometer selenium as described in any one of claim 1~8 is prepared.
10. nanometer selenium as claimed in claim 9, it is characterised in that the particle diameter of the nanometer selenium is 30~40nm.
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| CN201710046335.0A CN106801069A (en) | 2017-01-22 | 2017-01-22 | Nano selenium and preparation method thereof |
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| CN107758628A (en) * | 2017-08-31 | 2018-03-06 | 华南农业大学 | A kind of nanometer selenium for preparing the method for nanometer selenium using camellia plant water extract and being prepared |
| CN108522841A (en) * | 2018-03-19 | 2018-09-14 | 牡丹江市艾利维亚生物科技有限公司 | A kind of animals and plants nanometer selenium peptide nutrient solution and preparation method thereof |
| CN113396965A (en) * | 2021-06-17 | 2021-09-17 | 浙江工商大学 | Nutritional nano biological composite preservative, preparation method thereof and application of preservative to preservation of Taihu whitebait |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107758628A (en) * | 2017-08-31 | 2018-03-06 | 华南农业大学 | A kind of nanometer selenium for preparing the method for nanometer selenium using camellia plant water extract and being prepared |
| CN107758628B (en) * | 2017-08-31 | 2020-10-09 | 华南农业大学 | Method for preparing nano-selenium by using camellia plant water extract and nano-selenium prepared by method |
| CN108522841A (en) * | 2018-03-19 | 2018-09-14 | 牡丹江市艾利维亚生物科技有限公司 | A kind of animals and plants nanometer selenium peptide nutrient solution and preparation method thereof |
| CN113396965A (en) * | 2021-06-17 | 2021-09-17 | 浙江工商大学 | Nutritional nano biological composite preservative, preparation method thereof and application of preservative to preservation of Taihu whitebait |
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