CN103120270A - Rotifer strengthening method for stimulating eriocheir sinensis larva growth - Google Patents
Rotifer strengthening method for stimulating eriocheir sinensis larva growth Download PDFInfo
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- CN103120270A CN103120270A CN2013100825048A CN201310082504A CN103120270A CN 103120270 A CN103120270 A CN 103120270A CN 2013100825048 A CN2013100825048 A CN 2013100825048A CN 201310082504 A CN201310082504 A CN 201310082504A CN 103120270 A CN103120270 A CN 103120270A
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- chlorella
- manganese
- eriocheir sinensis
- wheel animalcule
- copper
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Abstract
The invention discloses a practical rotifer strengthening method for medium trace elements-copper and manganese in eriocheir sinensis larva. The method specifically comprises the steps of preparing highly concentrated chlorella (the wet weight is (2-8)*1010 cells/g), adding a copper sulphate solution with the concentration of 1000-2000mg/L and a manganese sulfate solution with the concentration of 500-1000mg/L, enriching a marine chlorella solution with the total Cu amount of 400-800ug/g and a marine chlorella solution with the total Mg amount of 150-300ug/g for 3-6 hours, and using the enriched marine chlorella for strengthening the rotifers for 10-12h, wherein the quantity relative ratio of the chlorella to the rotifers is 5*103 cells/ind. The rotifers strengthened by the method are used for feeding the eriocheir sinensis larva, so that the trace elements, copper and manganese, are effectively supplemented, metamorphosis development can be stimulated, the eriocheir sinensis larva are developed into megalopa in a metamorphosis manner before a natural state, and the artificial breeding yield and quality of eriocheir sinensis megalopa are increased.
Description
Technical field
The present invention relates to a kind of wheel animalcule fortification method, particularly relate to a kind of trace copper of Eriocheir Sinensis growth and method of manganese strengthened wheel animalcule of promoting.
Background technology
The artificial breeding Eriocheir Sinensis needs a large amount of living body baits, and the wheel animalcule of artificial culture is generally as the living body bait of shrimp Crab larvae.In propagating process artificially, the nutrient balance of the open-mouthed bait of stage of development plays a key effect to the seed output in later stage.Trace element can be kept skeleton development as prothetic group and the metal binding site of some enzymes, promotes human body immune function and keeps normally carrying out of metabolism.The content requirement that Eriocheir Sinensis is grown copper in bait and manganese is respectively 40 mg/kg, 17 mg/kg, and the wheel animalcule of artificial culture is not having under the replenishing of extra nutrient, the content of copper and manganese is only 6 mg/kg, 9 mg/kg, far below the demand of growing of Eriocheir Sinensis.Eriocheir Sinensis is to propagate the living body bait kind of ingesting under environment artificially limited, and practice is produced usually throwing something and feeding after the fortification of living body bait wheel animalcule, but after strengthening, marked change does not occur the content of trace element.
The method of fortification wheel animalcule has multiple at present, its cost and effect are also had nothing in common with each other, be comparatively common schedule of reinforcement to the wheel animalcule Micro Algae of throwing something and feeding, existing intensifying method only is confined to replenish aliphatic acid and vitamin in the living body bait wheel animalcule, has often ignored replenishing of external source trace element.Copper and manganese are all water-soluble substanceses, and wheel animalcule can not absorb trace copper and manganese in water body.So far, the method for aliphatic acid and vitamin enrichment wheel animalcule can not be applied directly to trace quantity mineral substance element, and the intensifying method of water soluble trace elements is not yet set up.So, seek wheel animalcule trace element reinforcement technique, to satisfy the Eriocheir Sinensis growth requirement, to become the problem of needing solution badly.
Summary of the invention
The purpose of this invention is to provide a kind of practical approach for Eriocheir Sinensis trace copper and manganese strengthened wheel animalcule, make the ratio of the living body bait wheel animalcule nutrient of Eriocheir Sinensis reach balance, to satisfy the demand of growing of Eriocheir Sinensis.
It is the Rich in Trace Element carrier that method of the present invention adopts marine chlorella, first uses the marine chlorella Rich in Trace Element, then strengthens wheel animalcule with it.
Be specially: configuration height concentrates chlorella (weight in wet base, 2-8 * 10
10Cells g
1), adding concentration is that 1000-2000 mg/L copper sulphate and concentration are 500-1000 mg/L manganese sulfate solution, the total amount that makes Cu is that the total amount of 400-800 ug/g marine chlorella solution, Mn is 150-300ug/g marine chlorella solution, enrichment 3-6 h, to be used for strengthen wheel animalcule 10-12 h through the marine chlorella after enrichment, the amount of chlorella and wheel animalcule is than 5 * 10
3Cells/ ind.
In said method, copper used can be the cupric sulfate pentahydrate AR, and manganese can be analytical pure sulfuric acid manganese reagent.In wheel animalcule after strengthening through said method, the content of Cu and Mn can reach respectively 40-60 mg/kg, 15-25 mg/kg, can satisfy the demand of growing of Eriocheir Sinensis, and can not produce toxic reaction.
In said method, the process of chlorella enriching Cu, manganese can be carried out simultaneously, also can carry out successively respectively, and as first enriching Cu enrichment manganese again, or first enrichment manganese enriching Cu again.
Further, available through the said method enrichment chlorella, aliphatic acid and the vitamin of copper and manganese strengthen simultaneously wheel animalcule, make wheel animalcule endotrophic material proportion balance.
Feed Eriocheir Sinensis with the wheel animalcule that method of the present invention is strengthened, trace copper and manganese have effectively been replenished, can promote its development by metamorphosis, make it than abnormal to megalops larva in advance under nature, improve output and the quality of artificial breeding Eriocheir sinensis megalopa.
Description of drawings
Fig. 1 takes turns the interior copper manganese content of polypide with the change curve of enhanced time in embodiment 3;
Fig. 2 takes turns the interior n-3 HUPA content of polypide with the change curve of enhanced time in embodiment 3;
Fig. 3 is paedomorphosis index column comparison diagram.
The specific embodiment
The present invention is further described below in conjunction with embodiment, but be not construed as limiting the invention.
It is the tank of 30L that volume is adopted in test, and the 25 L salinity of packing into are 20 ‰ seawater, and it is 50 μ g L that tank arranges interpolation copper concentration
1, the concentration of manganese is 50 ug L
-1, the control group that does not add copper, manganese is set, with 150 mesh sieve thin,tough silk, the wheel animalcule of cultivating is condensed into high density wheel animalcule liquid, then get 1mL dilution counting, being adjusted to density is 800 every milliliter, take concentration as every milliliter 4 * 10
6The chlorella cells wheel animalcule of throwing something and feeding is kept its normal physiological state.The concentration of measuring the interior copper of wheel polypide and manganese after 12 hours is respectively 6.48 ± 0.2 and 9.50 ± 0.2 μ g g
1(dry), comparing with control group does not have significant change.
Marine chlorella all in results exponential phase of growth, through LXJ-H type centrifuge centrifugal concentrating, is stored in refrigerator and (is no more than 24 hours).Add appropriate algae mud with the wheel animalcule nutrient solution, configuration height concentrates chlorella (weight in wet base, 7 * 10
10Cells/g); Be configured to concentration with deionized water and be respectively 1000mg/L, 1500mg, 2000 mg/L copper-baths and 500mg/L, 750mg/L, 1000 mg/L manganese sulfate solutions.Get 8 plastic test tubes and be numbered 1-8, respectively add 10 g chlorella solution, adding successively respectively 4mL concentration in the 1-4 test tube is 0mg/L, 1000mg/L, 1500mg, 2000 mg/L copper-baths, adding successively respectively 3mL concentration is 0mg/L, 500mg/L, 750mg/L, 1000 mg/L manganese sulfate solutions again, test tube is positioned under 23 ℃, made in the 1-4 test tube chlorella Rich in Trace Element copper and manganese 3 hours.Chlorella solution 720 after enrichment is finished
gCentrifugal 20min removes solution, collects chlorella and detects the wherein content of trace copper and manganese, and result is as follows:
In the embodiment 2 enrichment cross No. 3 chlorella test tubes of trace copper and manganese and 2 mg/L oil-in-water type fish oil strengthen body length as the brachionus plicatilis of 200-300 um (
Bracgionus plicatilis), the amount of 25L chlorella and wheel animalcule is than 5 * 10
3Cells/ ind(adds 50 mg oil-in-water type fish oil), make its body contain suitable trace element and unrighted acid.Respectively at sampling in 1,3,6,12,24 hour, according to wheel animalcule body fat acid content and micronutrient levels after GB/T5009. 168-2003 and GB/T5009.17-2003 detection fortifications, result as shown in Figure 1, 2.By in figure as can be known, main n-3 higher unsaturated fatty acid (high unsaturated fatty acid in the wheel polypide, HUPA) and the content of trace copper and manganese obviously rise, when enhanced time is 12h, substantially reach Mitten Crab Larvae to the nutritional need of trace copper and manganese and aliphatic acid in the wheel polypide, the long meeting of enhanced time causes the cost of industrial seedling rearing to increase.
The impact on the paedomorphosis of Sinensis huge legendary turtle crab of embodiment 4 trace coppers and manganese strengthened wheel animalcule
In industrial seedling rearing, after wheel animalcule was strengthened by method as described in Example 3, to 1 phase zoea, the 15/L of wheel animalcule that throws something and feeds every day, 2 phase zoeas, the 20/L of wheel animalcule that throws something and feeds every day threw something and fed 8 days, altogether to throw something and feed without the wheel animalcule of strengthening as contrast.As shown in Figure 3, the development condition of the young shows, compares with control group, and copper and manganese strengthened wheel animalcule have significant facilitation to the developmental index of the young, can promote the molt metamorphosis of megalops larva, and then reduces the generation of Mitten Crab Larvae autotomy phenomenon.
Claims (3)
1. wheel animalcule intensifying method that promotes that Eriocheir Sinensis is grown, it is characterized in that: described method is: configuration concentration is 2-8 * 10
10Cells g
1(weight in wet base) chlorella, adding concentration is that 1000-2000 mg/L copper sulphate and concentration are 500-1000 mg/L manganese sulfate solution, the total amount that makes Cu is that the total amount of 400-800 ug/g marine chlorella solution, Mn is 150-300ug/g marine chlorella solution, enrichment 3-6 h, to be used for strengthen wheel animalcule 10-12 h through the marine chlorella after enrichment, the amount of chlorella and wheel animalcule is than 5 * 10
3Cells/ ind.
2. the method for claim 1, it is characterized in that: the process of described chlorella enriching Cu, manganese can be carried out simultaneously, also can carry out successively respectively, as first enriching Cu enrichment manganese again, or first enrichment manganese enriching Cu again.
3. the method for claim 1, is characterized in that: also comprise with aliphatic acid and vitamin enrichment wheel animalcule.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103284010A (en) * | 2013-06-24 | 2013-09-11 | 宿迁洪祥饲料科技有限公司 | Formula and preparation method of eriocheir sinensis ecology starter feed |
| CN106386595A (en) * | 2016-08-31 | 2017-02-15 | 中国水产科学研究院淡水渔业研究中心 | Microelement nutrition reinforcing method capable of promoting development of larval of Macrobrachium nipponense |
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| CN102106278A (en) * | 2010-12-30 | 2011-06-29 | 广东海洋大学 | Method for breeding marine crabs artificially |
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2013
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106278A (en) * | 2010-12-30 | 2011-06-29 | 广东海洋大学 | Method for breeding marine crabs artificially |
Non-Patent Citations (4)
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| 卢彩霞: "铜、铁、锰、锌四种微量元素对花（鱼骨）生长和主要生理机能的影响", 《中国优秀硕士学位论文全文数据库》, 24 August 2009 (2009-08-24), pages 2 - 4 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103284010A (en) * | 2013-06-24 | 2013-09-11 | 宿迁洪祥饲料科技有限公司 | Formula and preparation method of eriocheir sinensis ecology starter feed |
| CN106386595A (en) * | 2016-08-31 | 2017-02-15 | 中国水产科学研究院淡水渔业研究中心 | Microelement nutrition reinforcing method capable of promoting development of larval of Macrobrachium nipponense |
| CN106386595B (en) * | 2016-08-31 | 2019-05-10 | 中国水产科学研究院淡水渔业研究中心 | A kind of micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis |
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